Moc08g35550 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g35550
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionReverse transcriptase domain-containing protein
Locationchr8: 26118750 .. 26119724 (-)
RNA-Seq ExpressionMoc08g35550
SyntenyMoc08g35550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGCATCTTACCTTGGCCTACCTCTACATGGCAAGCCTCAATCAGCATCTTTTTGGACCCGGTCATGGAGAAAGTGAGGAAAAGACTTAGTTCATGGGATCACTCCATCATCTCGAAAGGAGGTCAGCTCACCCTCATCCAAGCCACTTTATCCAATCTACCCACATATTACTTATCCATTTTCTCTATCCCAACTAAAGTGGCAAAGGATTTGGAAAAACTCATCCGAAATTTTTTGTGGAAGGGTTCAAACAACAACGGGGCACAAAATTTGGTTAGATGGGATCAAGTACAGAAACCAATTGAAGAAGGTGGGCTGGGTATATGCTGTATCAAGGACAAAAATCGGGCTCTTTTAGCCAAGTGGAGTTGGAGATTTCTTTTGGAAGAAACTGCCCTTTGGAGGAGAGTGATTTTGGCAAAATATGCCTTCTCCGACCACCGAAGTTCTCACCCAAACCGGATTTTTGCATCAACATAAGGTTTATGGAGAGACATTGACAAGCAGAAATCCTTGATCTTTGACAGAATCACTTATAAAGTGGGTACAGGCTTCCTTCCAAGGTTCTGGATTGATGTTTGGCTCACCAGAGAACCCCTCTGCCAAGCCTACCCCGTTCTATTTTTCTTACACTCCAGAAAGTTGGGCTCTGTAAAAGATTTTTGGTCCTCGTCGGATAAGGTTTGGGACATCCAATTTCGAAGGCACCTAAAGGATGCTGAGATTGATGAGCTAGCATCTCTCCTCCACAATCTTTCCTCCATCAGCCCTTCTACCAATCGGGATACTATTATTTGGAGTTTGGAATCACATGGACAATTCGCCACCTCCTCCCTCATTAAAGACATCCACCAAACAACAACATCACAGACAACTTCCTCTCTGTACAGTGCTATCTGGAAAGATCACTACCCAAGGAAGATAAAGATCTTCCTATGGGAAACTAGTCTCAATGCTATCAATACATAG

mRNA sequence

ATGGCCGGCATCTTACCTTGGCCTACCTCTACATGGCAAGCCTCAATCAGCATCTTTTTGGACCCGGTCATGGAGAAAGTGAGGAAAAGACTTAGTTCATGGGATCACTCCATCATCTCGAAAGGAGGTCAGCTCACCCTCATCCAAGCCACTTTATCCAATCTACCCACATATTACTTATCCATTTTCTCTATCCCAACTAAAGTGGCAAAGGATTTGGAAAAACTCATCCGAAATTTTTTGTGGAAGGGTTCAAACAACAACGGGGCACAAAATTTGGTTAGATGGGATCAAGTACAGAAACCAATTGAAGAAGGTGGGCTGGGTATATGCTGTATCAAGGACAAAAATCGGGCTCTTTTAGCCAAGTGGAGTTGGAGATTTCTTTTGGAAGAAACTGCCCTTTGGAGGAGAGTGATTTTGGCAAAATATGCCTTCTCCGACCACCGAAGCTTCCTTCCAAGGTTCTGGATTGATGTTTGGCTCACCAGAGAACCCCTCTGCCAAGCCTACCCCGTTCTATTTTTCTTACACTCCAGAAAGTTGGGCTCTGTAAAAGATTTTTGGTCCTCGTCGGATAAGGTTTGGGACATCCAATTTCGAAGGCACCTAAAGGATGCTGAGATTGATGAGCTAGCATCTCTCCTCCACAATCTTTCCTCCATCAGCCCTTCTACCAATCGGGATACTATTATTTGGAGTTTGGAATCACATGGACAATTCGCCACCTCCTCCCTCATTAAAGACATCCACCAAACAACAACATCACAGACAACTTCCTCTCTGTACAGTGCTATCTGGAAAGATCACTACCCAAGGAAGATAAAGATCTTCCTATGGGAAACTAGTCTCAATGCTATCAATACATAG

Coding sequence (CDS)

ATGGCCGGCATCTTACCTTGGCCTACCTCTACATGGCAAGCCTCAATCAGCATCTTTTTGGACCCGGTCATGGAGAAAGTGAGGAAAAGACTTAGTTCATGGGATCACTCCATCATCTCGAAAGGAGGTCAGCTCACCCTCATCCAAGCCACTTTATCCAATCTACCCACATATTACTTATCCATTTTCTCTATCCCAACTAAAGTGGCAAAGGATTTGGAAAAACTCATCCGAAATTTTTTGTGGAAGGGTTCAAACAACAACGGGGCACAAAATTTGGTTAGATGGGATCAAGTACAGAAACCAATTGAAGAAGGTGGGCTGGGTATATGCTGTATCAAGGACAAAAATCGGGCTCTTTTAGCCAAGTGGAGTTGGAGATTTCTTTTGGAAGAAACTGCCCTTTGGAGGAGAGTGATTTTGGCAAAATATGCCTTCTCCGACCACCGAAGCTTCCTTCCAAGGTTCTGGATTGATGTTTGGCTCACCAGAGAACCCCTCTGCCAAGCCTACCCCGTTCTATTTTTCTTACACTCCAGAAAGTTGGGCTCTGTAAAAGATTTTTGGTCCTCGTCGGATAAGGTTTGGGACATCCAATTTCGAAGGCACCTAAAGGATGCTGAGATTGATGAGCTAGCATCTCTCCTCCACAATCTTTCCTCCATCAGCCCTTCTACCAATCGGGATACTATTATTTGGAGTTTGGAATCACATGGACAATTCGCCACCTCCTCCCTCATTAAAGACATCCACCAAACAACAACATCACAGACAACTTCCTCTCTGTACAGTGCTATCTGGAAAGATCACTACCCAAGGAAGATAAAGATCTTCCTATGGGAAACTAGTCTCAATGCTATCAATACATAG

Protein sequence

MAGILPWPTSTWQASISIFLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLEKLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETALWRRVILAKYAFSDHRSFLPRFWIDVWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNLSSISPSTNRDTIIWSLESHGQFATSSLIKDIHQTTTSQTTSSLYSAIWKDHYPRKIKIFLWETSLNAINT
Homology
BLAST of Moc08g35550 vs. NCBI nr
Match: KAA0044556.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-52
Identity = 117/312 (37.50%), Postives = 168/312 (53.85%), Query Frame = 0

Query: 15  SISIFLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLE 74
           S S F D +++K++K+LSSW +S +SKGG++TLI +TL +LP Y LS+F +P  +A+ +E
Sbjct: 489 SSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGIAQKIE 548

Query: 75  KLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETA 134
              RNFLW G++N    +L+RW+QV  P E+GGLGI  +   N ALL KW W+FL E+  
Sbjct: 549 AYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHFVHSTNFALLCKWLWKFLTEKEP 608

Query: 135 LWRRVILAKYAFSDHRSFLPR-----------------------------------FWID 194
           LW+R+I++KY       F  R                                   FW+D
Sbjct: 609 LWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGEDISFWLD 668

Query: 195 VWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNL 254
            W    PL  A P LF L + K GSVKD W+ S K W+I   R L+D E +   ++  +L
Sbjct: 669 NWNGNSPLSLAVPRLFALSTNKKGSVKDLWNPSLKDWNIHVNRPLRDHEKNLWHNIKASL 728

Query: 255 SSISPSTNRDTIIWSLESHGQFATSSLIKDIHQTTTSQTT--SSLYSAIWKDHYPRKIKI 290
            +  P       +W L S+  F T+S+ KD+ + + S T    SLY  +WK  +P+K K 
Sbjct: 729 PTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPTNFHPSLYKTLWKVDFPKKCKF 788

BLAST of Moc08g35550 vs. NCBI nr
Match: KAA0041397.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 216.9 bits (551), Expect = 2.4e-52
Identity = 116/312 (37.18%), Postives = 167/312 (53.53%), Query Frame = 0

Query: 15  SISIFLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLE 74
           S S F D +++K++K+LSSW +S +SKGG++TLI +TL +LP Y LS+F +P  +A+ +E
Sbjct: 583 SSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGIAQKIE 642

Query: 75  KLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETA 134
              RNFLW G++N    +L+RW+QV  P E+GGLGI  +   N ALL KW W+FL E+  
Sbjct: 643 AYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHSVHSTNFALLCKWLWKFLTEKEP 702

Query: 135 LWRRVILAKYAFSDHRSFLPR-----------------------------------FWID 194
           LW+R+I++KY       F  R                                   FW+D
Sbjct: 703 LWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGEDISFWLD 762

Query: 195 VWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNL 254
            W    PL    P LF L + K GSVKD W+ S K W+I   R L+D E +   ++  +L
Sbjct: 763 NWNGNSPLSLVVPRLFALSTNKKGSVKDLWNPSLKDWNIHVNRPLRDHEKNLWHNIKASL 822

Query: 255 SSISPSTNRDTIIWSLESHGQFATSSLIKDIHQTTTSQTT--SSLYSAIWKDHYPRKIKI 290
            +  P       +W L S+  F T+S+ KD+ + + S T    SLY  +WK  +P+K K 
Sbjct: 823 PTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPTNFHPSLYKTLWKVDFPKKCKF 882

BLAST of Moc08g35550 vs. NCBI nr
Match: KAA0039950.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24553.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 211.5 bits (537), Expect = 9.9e-51
Identity = 111/312 (35.58%), Postives = 168/312 (53.85%), Query Frame = 0

Query: 15   SISIFLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLE 74
            S S F D V++K++K+LS+W +S +SKGG++TLI +TL +LP Y +S+F +P  +A+ +E
Sbjct: 691  SSSNFWDNVLQKIQKKLSNWKYSQLSKGGRITLINSTLESLPIYQMSVFKVPKGIAQKIE 750

Query: 75   KLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETA 134
               RNFLW G++N    +L+RW+Q+  P E+GGLGI  +   N ALL KW W+FL E+  
Sbjct: 751  ASWRNFLWNGASNGHNISLIRWNQIVSPKEKGGLGIHSVNSTNFALLCKWLWKFLTEKDP 810

Query: 135  LWRRVILAKY------AFSDHRSFLPR-----------------------------FWID 194
            LW+R+I++KY      +F  H  F                                FW+D
Sbjct: 811  LWKRLIISKYDKEKMGSFPSHGKFSSNNSPWKAVTECISWFYKNISWKVNDGEDISFWLD 870

Query: 195  VWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNL 254
             W    PL  A P LF L + K GSVK+FW+ S   W +   R L+D E +   ++  +L
Sbjct: 871  NWNGNAPLSLAVPRLFALSTNKKGSVKEFWNPSSNDWHLHINRPLRDHEENLWHNIKASL 930

Query: 255  SSISPSTNRDTIIWSLESHGQFATSSLIKDIHQTTTSQTT--SSLYSAIWKDHYPRKIKI 290
             +  P+      +W+L S+  F T+S+ + I +   S      +LY  +WK  +P+K K 
Sbjct: 931  PTPLPNRGHPKPLWNLNSNNIFDTASVKRAIAEAPISPANFHPNLYKTLWKVEFPKKCKF 990

BLAST of Moc08g35550 vs. NCBI nr
Match: VVA30963.1 (Hypothetical predicted protein, partial [Prunus dulcis])

HSP 1 Score: 205.7 bits (522), Expect = 5.4e-49
Identity = 119/309 (38.51%), Postives = 166/309 (53.72%), Query Frame = 0

Query: 19  FLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLEKLIR 78
           F +PV +KV KRL  W  + +SKGG+LTLIQA LS++P+YY+S+F +PT VA  +E+L+R
Sbjct: 89  FWNPVKDKVEKRLQKWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPTGVAAKVEQLMR 148

Query: 79  NFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETALWRR 138
           NFLW+G       +LVRW++V K  EEGGLGI  ++++N AL AKW WRF LE  +LW R
Sbjct: 149 NFLWEGLEEGKKCHLVRWERVTKSKEEGGLGIGSLRERNEALRAKWLWRFPLETNSLWHR 208

Query: 139 VILAKYAFSD-----------------------HRSFLP------------RFWIDVWLT 198
           +I +KY                           + SFL             RFW D+WL 
Sbjct: 209 IIKSKYGIDSNGWDTKQIVKVSCRNPWREISKGYNSFLQCCRFSVGNGEKIRFWEDLWLK 268

Query: 199 REPLCQAYPVLFFLHSRKLGSVKDFWSSSDKV--WDIQFRRHLKDAEIDELASLLHNLSS 258
              L   +P L  L  RK  S+  F ++ +    WD  FRR+L +AEI E+  LL  L +
Sbjct: 269 EGILKDLFPRLSSLSRRKNQSIARFANNHEMPLNWDFDFRRNLSEAEIAEVVILLDILGN 328

Query: 259 ISPSTNR-DTIIWSLESHGQFATSSLIKDIHQTTTSQTTSSLYSAIWKDHYPRKIKIFLW 290
           +    +R D   W +E  G F+  S    +  TT  +     +S+IWK   P KI+ F+W
Sbjct: 329 VRLYGSRPDRRSWEIEEQGSFSCKSFRSFLLSTT--RDVFPPFSSIWKAKTPPKIQFFVW 388

BLAST of Moc08g35550 vs. NCBI nr
Match: TQD93576.1 (hypothetical protein C1H46_020784 [Malus baccata])

HSP 1 Score: 202.2 bits (513), Expect = 6.0e-48
Identity = 117/310 (37.74%), Postives = 164/310 (52.90%), Query Frame = 0

Query: 19  FLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLEKLIR 78
           F DPV+EK+ KRL SW  + +S+GG+LTLIQ+ L +LP YY+S+F IP  V   LEKL++
Sbjct: 59  FWDPVVEKMEKRLQSWKKAFLSRGGRLTLIQSVLGSLPIYYMSLFKIPCGVIGRLEKLMK 118

Query: 79  NFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETALWRR 138
            FLW+G       +LV+W+ V K  EEGGLG+  ++++N ALLAKW WRF  E  +LW +
Sbjct: 119 GFLWEGVEEGKKNHLVKWEIVIKSKEEGGLGVGNLRNRNEALLAKWLWRFPKEPHSLWHK 178

Query: 139 VILAKYAFSDH--RSFLP---------------------------------RFWIDVWLT 198
           VI +KY   ++   +F P                                 RFW D WL 
Sbjct: 179 VIRSKYGLQNNGWNAFPPMRGSSRSPWKDISSGSQLFLHCCKFEVGNGERVRFWEDGWLE 238

Query: 199 REPLCQAYPVLFFL---HSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNLS 258
             PL + +P LF L   H++ + S  DF S++   W+  FRR+L +AEI+E A LL  + 
Sbjct: 239 GGPLKEQFPRLFLLSRMHNQNISSFADF-STNSLSWNFDFRRNLNEAEIEEAARLLQKVE 298

Query: 259 SISPSTNR-DTIIWSLESHGQFATSSLIKDIHQTTTSQTTSSLYSAIWKDHYPRKIKIFL 290
            +  S +R D   W LE+   F   S    +      Q     YS IWK   P K+KI +
Sbjct: 299 EVRLSQSRTDNRRWKLEASRLFTCKSYRSFLSNNGIMQYFPP-YSQIWKSKVPPKVKILV 358

BLAST of Moc08g35550 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.4e-28
Identity = 84/311 (27.01%), Postives = 139/311 (44.69%), Query Frame = 0

Query: 23  VMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLEKLIRNFLW 82
           ++E+V  R+S W    +S  G+LTL +A LS++P + +S   +P  +   L++L R FLW
Sbjct: 16  ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 83  KGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETALWRRVILA 142
             +     Q+LV+W +V  P +EGGLG+   K  NRAL++K  WR L E+ +LW  V+  
Sbjct: 76  GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 143 KY---AFSDHRSFLP---------------------------------RFWIDVWLTREP 202
           KY      D R  +P                                 RFW D W++ +P
Sbjct: 136 KYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKP 195

Query: 203 LCQAYPVLFFLHSRKLGS------VKDFWSSSDKVWDI-QFRRHLKDAEIDELASLLHNL 262
           L +       L + +  +       KD W    + WD  +   +  +    EL +++ +L
Sbjct: 196 LLE-------LDNGERPTDCDTVVAKDLWIPG-RGWDFAKIDPYTTNNTRLELRAVVLDL 255

Query: 263 SSISPSTNRDTIIWSLESHGQFATSSLIKDIH-QTTTSQTTSSLYSAIWKDHYPRKIKIF 290
            + +    RD + W     GQF+  S  + +          +S ++ +WK   P ++K F
Sbjct: 256 VTGA----RDRLSWKFSQDGQFSVRSAYEMLTVDEVPRPNMASFFNCLWKVRVPERVKTF 314

BLAST of Moc08g35550 vs. ExPASy TrEMBL
Match: A0A5A7TR15 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003050 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 5.1e-53
Identity = 117/312 (37.50%), Postives = 168/312 (53.85%), Query Frame = 0

Query: 15  SISIFLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLE 74
           S S F D +++K++K+LSSW +S +SKGG++TLI +TL +LP Y LS+F +P  +A+ +E
Sbjct: 489 SSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGIAQKIE 548

Query: 75  KLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETA 134
              RNFLW G++N    +L+RW+QV  P E+GGLGI  +   N ALL KW W+FL E+  
Sbjct: 549 AYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHFVHSTNFALLCKWLWKFLTEKEP 608

Query: 135 LWRRVILAKYAFSDHRSFLPR-----------------------------------FWID 194
           LW+R+I++KY       F  R                                   FW+D
Sbjct: 609 LWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGEDISFWLD 668

Query: 195 VWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNL 254
            W    PL  A P LF L + K GSVKD W+ S K W+I   R L+D E +   ++  +L
Sbjct: 669 NWNGNSPLSLAVPRLFALSTNKKGSVKDLWNPSLKDWNIHVNRPLRDHEKNLWHNIKASL 728

Query: 255 SSISPSTNRDTIIWSLESHGQFATSSLIKDIHQTTTSQTT--SSLYSAIWKDHYPRKIKI 290
            +  P       +W L S+  F T+S+ KD+ + + S T    SLY  +WK  +P+K K 
Sbjct: 729 PTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPTNFHPSLYKTLWKVDFPKKCKF 788

BLAST of Moc08g35550 vs. ExPASy TrEMBL
Match: A0A5A7TIB8 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00430 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.1e-52
Identity = 116/312 (37.18%), Postives = 167/312 (53.53%), Query Frame = 0

Query: 15  SISIFLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLE 74
           S S F D +++K++K+LSSW +S +SKGG++TLI +TL +LP Y LS+F +P  +A+ +E
Sbjct: 583 SSSNFWDNILQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQLSVFKVPKGIAQKIE 642

Query: 75  KLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETA 134
              RNFLW G++N    +L+RW+QV  P E+GGLGI  +   N ALL KW W+FL E+  
Sbjct: 643 AYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHSVHSTNFALLCKWLWKFLTEKEP 702

Query: 135 LWRRVILAKYAFSDHRSFLPR-----------------------------------FWID 194
           LW+R+I++KY       F  R                                   FW+D
Sbjct: 703 LWKRLIISKYDQEKMGRFPSRGKYSSNNSPWKAVTNCISWFYKNIGWKVNDGEDISFWLD 762

Query: 195 VWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNL 254
            W    PL    P LF L + K GSVKD W+ S K W+I   R L+D E +   ++  +L
Sbjct: 763 NWNGNSPLSLVVPRLFALSTNKKGSVKDLWNPSLKDWNIHVNRPLRDHEKNLWHNIKASL 822

Query: 255 SSISPSTNRDTIIWSLESHGQFATSSLIKDIHQTTTSQTT--SSLYSAIWKDHYPRKIKI 290
            +  P       +W L S+  F T+S+ KD+ + + S T    SLY  +WK  +P+K K 
Sbjct: 823 PTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPTNFHPSLYKTLWKVDFPKKCKF 882

BLAST of Moc08g35550 vs. ExPASy TrEMBL
Match: A0A5A7T9I7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00980 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 4.8e-51
Identity = 111/312 (35.58%), Postives = 168/312 (53.85%), Query Frame = 0

Query: 15   SISIFLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLE 74
            S S F D V++K++K+LS+W +S +SKGG++TLI +TL +LP Y +S+F +P  +A+ +E
Sbjct: 691  SSSNFWDNVLQKIQKKLSNWKYSQLSKGGRITLINSTLESLPIYQMSVFKVPKGIAQKIE 750

Query: 75   KLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETA 134
               RNFLW G++N    +L+RW+Q+  P E+GGLGI  +   N ALL KW W+FL E+  
Sbjct: 751  ASWRNFLWNGASNGHNISLIRWNQIVSPKEKGGLGIHSVNSTNFALLCKWLWKFLTEKDP 810

Query: 135  LWRRVILAKY------AFSDHRSFLPR-----------------------------FWID 194
            LW+R+I++KY      +F  H  F                                FW+D
Sbjct: 811  LWKRLIISKYDKEKMGSFPSHGKFSSNNSPWKAVTECISWFYKNISWKVNDGEDISFWLD 870

Query: 195  VWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSSDKVWDIQFRRHLKDAEIDELASLLHNL 254
             W    PL  A P LF L + K GSVK+FW+ S   W +   R L+D E +   ++  +L
Sbjct: 871  NWNGNAPLSLAVPRLFALSTNKKGSVKEFWNPSSNDWHLHINRPLRDHEENLWHNIKASL 930

Query: 255  SSISPSTNRDTIIWSLESHGQFATSSLIKDIHQTTTSQTT--SSLYSAIWKDHYPRKIKI 290
             +  P+      +W+L S+  F T+S+ + I +   S      +LY  +WK  +P+K K 
Sbjct: 931  PTPLPNRGHPKPLWNLNSNNIFDTASVKRAIAEAPISPANFHPNLYKTLWKVEFPKKCKF 990

BLAST of Moc08g35550 vs. ExPASy TrEMBL
Match: A0A5E4FU33 (zf-RVT domain-containing protein (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B035216 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 2.6e-49
Identity = 119/309 (38.51%), Postives = 166/309 (53.72%), Query Frame = 0

Query: 19  FLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLEKLIR 78
           F +PV +KV KRL  W  + +SKGG+LTLIQA LS++P+YY+S+F +PT VA  +E+L+R
Sbjct: 89  FWNPVKDKVEKRLQKWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPTGVAAKVEQLMR 148

Query: 79  NFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETALWRR 138
           NFLW+G       +LVRW++V K  EEGGLGI  ++++N AL AKW WRF LE  +LW R
Sbjct: 149 NFLWEGLEEGKKCHLVRWERVTKSKEEGGLGIGSLRERNEALRAKWLWRFPLETNSLWHR 208

Query: 139 VILAKYAFSD-----------------------HRSFLP------------RFWIDVWLT 198
           +I +KY                           + SFL             RFW D+WL 
Sbjct: 209 IIKSKYGIDSNGWDTKQIVKVSCRNPWREISKGYNSFLQCCRFSVGNGEKIRFWEDLWLK 268

Query: 199 REPLCQAYPVLFFLHSRKLGSVKDFWSSSDKV--WDIQFRRHLKDAEIDELASLLHNLSS 258
              L   +P L  L  RK  S+  F ++ +    WD  FRR+L +AEI E+  LL  L +
Sbjct: 269 EGILKDLFPRLSSLSRRKNQSIARFANNHEMPLNWDFDFRRNLSEAEIAEVVILLDILGN 328

Query: 259 ISPSTNR-DTIIWSLESHGQFATSSLIKDIHQTTTSQTTSSLYSAIWKDHYPRKIKIFLW 290
           +    +R D   W +E  G F+  S    +  TT  +     +S+IWK   P KI+ F+W
Sbjct: 329 VRLYGSRPDRRSWEIEEQGSFSCKSFRSFLLSTT--RDVFPPFSSIWKAKTPPKIQFFVW 388

BLAST of Moc08g35550 vs. ExPASy TrEMBL
Match: M5WJ76 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa015871mg PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 2.6e-49
Identity = 119/297 (40.07%), Postives = 165/297 (55.56%), Query Frame = 0

Query: 19   FLDPVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLEKLIR 78
            F +PVMEKV KRL  W  + +SKGG+LTLIQA LS++P+YY+S+F +P  VA  +E+L+R
Sbjct: 1059 FWNPVMEKVEKRLQKWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPIGVAAKVEQLMR 1118

Query: 79   NFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNRALLAKWSWRFLLEETALWRR 138
            NFLW+G +     +LVRW++V K  EEGGLGI  ++++  AL AKW WRF LE  +LW R
Sbjct: 1119 NFLWEGLDEGKKCHLVRWERVTKSKEEGGLGIGSLRERIEALRAKWLWRFPLETNSLWHR 1178

Query: 139  VILAKYAFSD-----------HRSFLP------------RFWIDVWLTREPLCQAYPVLF 198
            +I +KY               + SFL             RFW D+WL    L   +P L 
Sbjct: 1179 IIKSKYGIDSNGNPWREISKGYNSFLQCCRFSVGNGEKIRFWEDLWLKEGILKDLFPRLS 1238

Query: 199  FLHSRKLGSVKDFWSSS--DKVWDIQFRRHLKDAEIDELASLLHNLSSISPSTNR-DTII 258
             L  RK  S+  F ++      WD  FRR+L +AEI E+  LL  L ++    +R D   
Sbjct: 1239 SLSRRKNQSIACFANNHVLPLNWDFDFRRNLSEAEIAEVVILLDILGNVRLYGSRPDRRS 1298

Query: 259  WSLESHGQFATSSLIKDIHQTTTSQTTSSLYSAIWKDHYPRKIKIFLWETSLNAINT 290
            W +E  G F+  S    +  TT  +     +S+IWK   P KI+ F+W  +   INT
Sbjct: 1299 WEVEEQGSFSCKSFRSFLLSTT--RDVFPPFSSIWKAKTPPKIQFFVWLAANGRINT 1353

BLAST of Moc08g35550 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 90.1 bits (222), Expect = 3.1e-18
Identity = 69/282 (24.47%), Postives = 119/282 (42.20%), Query Frame = 0

Query: 22  PVMEKVRKRLSSWDHSIISKGGQLTLIQATLSNLPTYYLSIFSIPTKVAKDLEKLIRNFL 81
           P++EK+R R+  W    +S  G+L LI + + +L  +++S F +P+   K+++ +  +FL
Sbjct: 44  PLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFL 103

Query: 82  WKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIKDKNR---------ALLAKWSWRFLLEE 141
           W G   N  +  V W  V  P +EGGLGI  +K+ N+           L  W W+ +L+ 
Sbjct: 104 WSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEANKGSFWSISGNTTLGSWMWKKILKH 163

Query: 142 TALWRRVILAKYAFSDHRSFLPRFWIDVWLTREPLCQAYPVLFFLHSRKLGSVKDFWSSS 201
            AL    +        H      FW D W     + +   V        +G +    S +
Sbjct: 164 RALASGFV----KHDIHNGSNTSFWFDNW---SKIGRLIDVTGHRGCIDMG-ITLHASVA 223

Query: 202 DKVWDIQFRRHLKDAEI---DELASLLHNLSSISPSTNRDTIIWSLESHGQ-FATSSLIK 261
           + V + + RRH  D  +   D +A + H       ++  DT+ W  + +G  F      K
Sbjct: 224 EAVVNHRPRRHRHDTLLRIEDVIAEVRHQ----GLTSGEDTVRW--KGNGDIFKPCFNTK 283

Query: 262 DIHQTTTS-QTTSSLYSAIWKDHYPRKIKIFLWETSLNAINT 290
           +    T   +   + Y  +W  H   K  +  W    N + T
Sbjct: 284 ETWAATREPKLKVNWYKGVWFSHATPKYSVLAWIAIKNRLTT 311

BLAST of Moc08g35550 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 9.7e-12
Identity = 64/282 (22.70%), Postives = 104/282 (36.88%), Query Frame = 0

Query: 55  LPTYYLSIFSIPTKVAKDLEKLIRNFLWKGSNNNGAQNLVRWDQVQKPIEEGGLGICCIK 114
           LPTY ++ F +P  V K +  ++ +F W+        +   WD +     EGG+G   I+
Sbjct: 3   LPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIE 62

Query: 115 DKNRALLAKWSWRFLLEETALWRRVILAKY---------AFSDHRSFLPR---------- 174
             N ALL K  WR L    +L  +V  ++Y               SF+ +          
Sbjct: 63  AFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILR 122

Query: 175 --------------FWIDVWLTREPL-----CQAYPVLFFLHSRKLGSVKDFWSSSDKVW 234
                          W   WL  +P       Q  P   +     +  V D    S + W
Sbjct: 123 QGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGREW 182

Query: 235 DIQFRRHLKDAEIDELASLLHNLSSISPSTNR--DTIIWSLESHGQFATSS----LIKDI 288
               R+ + +    E+   L  +  + P   R  D+  W   S G +   S    L + I
Sbjct: 183 ----RKDVIEMLFPEVERKL--IGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQII 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0044556.11.1e-5237.50LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0041397.12.4e-5237.18LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0039950.19.9e-5135.58LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK245... [more]
VVA30963.15.4e-4938.51Hypothetical predicted protein, partial [Prunus dulcis][more]
TQD93576.16.0e-4837.74hypothetical protein C1H46_020784 [Malus baccata][more]
Match NameE-valueIdentityDescription
P0C2F69.4e-2827.01Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A5A7TR155.1e-5337.50LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7TIB81.1e-5237.18LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7T9I74.8e-5135.58LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5E4FU332.6e-4938.51zf-RVT domain-containing protein (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2... [more]
M5WJ762.6e-4940.07Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
Match NameE-valueIdentityDescription
AT3G24255.13.1e-1824.47RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G29090.19.7e-1222.70Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 19..256
NoneNo IPR availablePANTHERPTHR33116:SF33OS01G0885550 PROTEINcoord: 19..256

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g35550.1Moc08g35550.1mRNA