Moc08g35530 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g35530
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionGFO_IDH_MocA domain-containing protein
Locationchr8: 26073046 .. 26074481 (-)
RNA-Seq ExpressionMoc08g35530
SyntenyMoc08g35530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGAGGCCCCGATACGTTTCGGCATCCTCGGCTGCGCCGAGATAGCCAGAAAAGTGGCCAGAGCGATAAACTCAGCACCCAACTCCGCCGTCTCCGCCGTCGCCAGCCGCTCGCTCGACAAGGCCTTGAACTTCGCCGCCGTCAACGGTTTGCCGGACTCTGTCAAGGTGTACGGCAGCTACGACCAGATTCTCGACGACCCGTGTGTGGACGCCGTCTACTTGCCTTTGCCGACCAGCCTTCACCGGCGGTGGGCGATCTTGGCGGCTCAGAAGAAGAAGCACATTCTCTTGGAGAAACCCACGGCGCTGAACGTTGGAGAGCTTGATCAGATTCTTGATGCGTGTGAATCGAACGGCGTGCAGTTCATGGATGGATCAATGTGGCTGCATCACCCTAGAACTGCCAAAATGAAAGAGATTATCTCTGATTCAAACCTTTTTGGCAATGTTAACTATGTAAGAGCTTTTCCCAACTTATAAAATATCAGCTTTTTTTCTAATAAGTACTTGTCATTAGAAGTTAGAAGTAGAACAATGAGTGATGATTTGGCACGACGGAGTAGACATAGATAAACTAAAAGCAAGTGAGCCAAAAAATTCAAATTAAATGTAGTTCACCTGCGCACTTGTCTGAATCTTTTGACCCATCTGCAGTTTTTACCCTGAATACATTCTTTGATTCTACTAGTTGTCGAGTCATGTCATATTAATATTTAAATAACGATATTATTAACGGTAGGAACTTATTAGAAACATAAAATGAAATACTAACCACTAAGTTATAAGTGATAAGTATGATTTCTCTGTGCAATTTCAAGATTCACAGCACATCAACAACATCAGGAACTAGAGAGTTTCTTGAGGAAAACATAAGAGTAAAGCCAGACCTGGATGCTTTGGGTGCACTGGGAGACTTGGCATGGTACTGCATTGGAGCCATCTTGTGGGCAAAAGACTATGAATTACCAACACATGTCTGTGCACTACCAGATGTTACAAAGAACTCGGCAGGCGTTATCTTGTCATGCAGTGCCTCCATTCATTGGGGCAGAACATCTGCAACAGTTCACTGCTCTTTCCTATCTCATACCTCCATGGACATAGCCATATCTGGTTCCCGTGGATCACTAAGTGTTTATGACTTCATCATCCCTTACCAAGAGACTTCAGCATTGTTTGATTTCATCTCGGGAGCGGCGTTTGCAGAACTCCATATTGGATGGAACAAACGACCAGAGGAAGTTGAGGTAGGTACTGAGCTACCACAAGAGGCTCTGATGGTGGCAGAGTTCGCCAGGCTTGTAGGGGCCATAAGAGCAACTGGGTCTCGTCCTGATAGCAAGTGGCCACAGATAAGTAGAAAGACACAGCTAGTGATGGATGCTGTAAAAAAATCCATTGATGGTGGTTGTAAAACAGTGTATTTGTAG

mRNA sequence

ATGGGCGAGGCCCCGATACGTTTCGGCATCCTCGGCTGCGCCGAGATAGCCAGAAAAGTGGCCAGAGCGATAAACTCAGCACCCAACTCCGCCGTCTCCGCCGTCGCCAGCCGCTCGCTCGACAAGGCCTTGAACTTCGCCGCCGTCAACGGTTTGCCGGACTCTGTCAAGGTGTACGGCAGCTACGACCAGATTCTCGACGACCCGTGTGTGGACGCCGTCTACTTGCCTTTGCCGACCAGCCTTCACCGGCGGTGGGCGATCTTGGCGGCTCAGAAGAAGAAGCACATTCTCTTGGAGAAACCCACGGCGCTGAACGTTGGAGAGCTTGATCAGATTCTTGATGCGTGTGAATCGAACGGCGTGCAGTTCATGGATGGATCAATGTGGCTGCATCACCCTAGAACTGCCAAAATGAAAGAGATTATCTCTGATTCAAACCTTTTTGGCAATGTTAACTATATTCACAGCACATCAACAACATCAGGAACTAGAGAGTTTCTTGAGGAAAACATAAGAGTAAAGCCAGACCTGGATGCTTTGGGTGCACTGGGAGACTTGGCATGGTACTGCATTGGAGCCATCTTGTGGGCAAAAGACTATGAATTACCAACACATGTCTGTGCACTACCAGATGTTACAAAGAACTCGGCAGGCGTTATCTTGTCATGCAGTGCCTCCATTCATTGGGGCAGAACATCTGCAACAGTTCACTGCTCTTTCCTATCTCATACCTCCATGGACATAGCCATATCTGGTTCCCGTGGATCACTAAGTGTTTATGACTTCATCATCCCTTACCAAGAGACTTCAGCATTGTTTGATTTCATCTCGGGAGCGGCGTTTGCAGAACTCCATATTGGATGGAACAAACGACCAGAGGAAGTTGAGGTAGGTACTGAGCTACCACAAGAGGCTCTGATGGTGGCAGAGTTCGCCAGGCTTGTAGGGGCCATAAGAGCAACTGGGTCTCGTCCTGATAGCAAGTGGCCACAGATAAGTAGAAAGACACAGCTAGTGATGGATGCTGTAAAAAAATCCATTGATGGTGGTTGTAAAACAGTGTATTTGTAG

Coding sequence (CDS)

ATGGGCGAGGCCCCGATACGTTTCGGCATCCTCGGCTGCGCCGAGATAGCCAGAAAAGTGGCCAGAGCGATAAACTCAGCACCCAACTCCGCCGTCTCCGCCGTCGCCAGCCGCTCGCTCGACAAGGCCTTGAACTTCGCCGCCGTCAACGGTTTGCCGGACTCTGTCAAGGTGTACGGCAGCTACGACCAGATTCTCGACGACCCGTGTGTGGACGCCGTCTACTTGCCTTTGCCGACCAGCCTTCACCGGCGGTGGGCGATCTTGGCGGCTCAGAAGAAGAAGCACATTCTCTTGGAGAAACCCACGGCGCTGAACGTTGGAGAGCTTGATCAGATTCTTGATGCGTGTGAATCGAACGGCGTGCAGTTCATGGATGGATCAATGTGGCTGCATCACCCTAGAACTGCCAAAATGAAAGAGATTATCTCTGATTCAAACCTTTTTGGCAATGTTAACTATATTCACAGCACATCAACAACATCAGGAACTAGAGAGTTTCTTGAGGAAAACATAAGAGTAAAGCCAGACCTGGATGCTTTGGGTGCACTGGGAGACTTGGCATGGTACTGCATTGGAGCCATCTTGTGGGCAAAAGACTATGAATTACCAACACATGTCTGTGCACTACCAGATGTTACAAAGAACTCGGCAGGCGTTATCTTGTCATGCAGTGCCTCCATTCATTGGGGCAGAACATCTGCAACAGTTCACTGCTCTTTCCTATCTCATACCTCCATGGACATAGCCATATCTGGTTCCCGTGGATCACTAAGTGTTTATGACTTCATCATCCCTTACCAAGAGACTTCAGCATTGTTTGATTTCATCTCGGGAGCGGCGTTTGCAGAACTCCATATTGGATGGAACAAACGACCAGAGGAAGTTGAGGTAGGTACTGAGCTACCACAAGAGGCTCTGATGGTGGCAGAGTTCGCCAGGCTTGTAGGGGCCATAAGAGCAACTGGGTCTCGTCCTGATAGCAAGTGGCCACAGATAAGTAGAAAGACACAGCTAGTGATGGATGCTGTAAAAAAATCCATTGATGGTGGTTGTAAAACAGTGTATTTGTAG

Protein sequence

MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYGSYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDALGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL
Homology
BLAST of Moc08g35530 vs. NCBI nr
Match: XP_022143296.1 (uncharacterized oxidoreductase At4g09670-like [Momordica charantia])

HSP 1 Score: 721.1 bits (1860), Expect = 4.8e-204
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG
Sbjct: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
           SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN
Sbjct: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCS 240
           LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCS
Sbjct: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCS 240

Query: 241 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT 300
           FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT
Sbjct: 241 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT 300

Query: 301 ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 358
           ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL
Sbjct: 301 ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 357

BLAST of Moc08g35530 vs. NCBI nr
Match: KAG7017970.1 (putative oxidoreductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 638.6 bits (1646), Expect = 3.1e-179
Identity = 315/361 (87.26%), Postives = 337/361 (93.35%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M E PIRFGI+GCAEIARKVARAINSAPNS VSAVASRSLDKALNFAAVNGLPD+VKVYG
Sbjct: 1   MAENPIRFGIVGCAEIARKVARAINSAPNSTVSAVASRSLDKALNFAAVNGLPDTVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
            YDQILDDPCVDAVY+PLPTSLH+RWAILAA+KKKHILLEKPTAL+V ELD+ILDACESN
Sbjct: 61  GYDQILDDPCVDAVYMPLPTSLHQRWAILAAEKKKHILLEKPTALDVEELDKILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKEIISD+ LFG+VNYIHSTSTTSGT++FLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEIISDTKLFGHVNYIHSTSTTSGTKQFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTS----AT 240
           LGALGDLAWYCIGA+LWAKDY+LPT V ALPDVTKNSAGVILSC+AS+ WG++S    AT
Sbjct: 181 LGALGDLAWYCIGALLWAKDYKLPTQVHALPDVTKNSAGVILSCTASLQWGKSSNETFAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDIAISG+ GSL+VYDFIIPYQETSA F+F SGA FAELHIGWN RPEEV
Sbjct: 241 IHCSFLSHTSMDIAISGACGSLNVYDFIIPYQETSASFEFTSGAKFAELHIGWNTRPEEV 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  ELPQEALMVAEFARLVGAI+ TGSRPDSKWPQISRKTQLVMDAVKKSID  CK VY
Sbjct: 301 EVVNELPQEALMVAEFARLVGAIKTTGSRPDSKWPQISRKTQLVMDAVKKSIDSDCKPVY 360

BLAST of Moc08g35530 vs. NCBI nr
Match: XP_022983648.1 (uncharacterized oxidoreductase At4g09670-like [Cucurbita maxima])

HSP 1 Score: 638.6 bits (1646), Expect = 3.1e-179
Identity = 315/361 (87.26%), Postives = 337/361 (93.35%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M E PIRFGI+GCAEIARKVARAINSAPNS VSAVASRSLDKALNFAAVNGLP++VKVYG
Sbjct: 1   MAENPIRFGIVGCAEIARKVARAINSAPNSTVSAVASRSLDKALNFAAVNGLPETVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
            YDQILDDPCVDAVY+PLPTSLH+RWAILAA+KKKHILLEKPTAL+V ELD+ILDACESN
Sbjct: 61  GYDQILDDPCVDAVYMPLPTSLHQRWAILAAEKKKHILLEKPTALDVEELDKILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKEIISD+ LFG+VNYIHSTSTTSGT++FLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEIISDTKLFGHVNYIHSTSTTSGTKQFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGR----TSAT 240
           LGALGDLAWYCIGA+LWAKDY+LPT V ALPDVTKNSAGVILSC+AS+ WG+    TSAT
Sbjct: 181 LGALGDLAWYCIGALLWAKDYKLPTQVHALPDVTKNSAGVILSCTASLQWGKSSNETSAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDIAISG+ GSL+VYDFIIPYQETSA F+F SGA FAELHIGWN RPEEV
Sbjct: 241 IHCSFLSHTSMDIAISGACGSLNVYDFIIPYQETSASFEFTSGAKFAELHIGWNTRPEEV 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  ELPQEALMVAEFARLVGAI+ TGSRPDSKWPQISRKTQLVMDAVKKSID  CK VY
Sbjct: 301 EVVNELPQEALMVAEFARLVGAIKTTGSRPDSKWPQISRKTQLVMDAVKKSIDSDCKPVY 360

BLAST of Moc08g35530 vs. NCBI nr
Match: XP_022935172.1 (uncharacterized oxidoreductase At4g09670-like [Cucurbita moschata])

HSP 1 Score: 636.0 bits (1639), Expect = 2.0e-178
Identity = 314/361 (86.98%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M   PIRFGI+GCAEIARKVARAINSAPNS VSAVASRSLDKALNFAAVNGLPD+VKVYG
Sbjct: 1   MAGNPIRFGIVGCAEIARKVARAINSAPNSTVSAVASRSLDKALNFAAVNGLPDTVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
            YDQILDDPCVDAVY+PLPTSLH+RWAILAA+KKKHILLEKPTAL+V ELD+ILDACESN
Sbjct: 61  GYDQILDDPCVDAVYMPLPTSLHQRWAILAAEKKKHILLEKPTALDVEELDKILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKEIISD+ LFG+VNYIHSTSTTSGT++FLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEIISDTKLFGHVNYIHSTSTTSGTKQFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTS----AT 240
           LGALGDLAWYCIGA+LWAKDY+LPT V ALPDVTKNSAGVILSC+AS+ WG++S    AT
Sbjct: 181 LGALGDLAWYCIGALLWAKDYKLPTQVHALPDVTKNSAGVILSCTASLQWGKSSNETFAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDIAISG+ GSL+VYDFIIPYQETSA F+F SGA FAELHIGWN RPEEV
Sbjct: 241 IHCSFLSHTSMDIAISGACGSLNVYDFIIPYQETSASFEFTSGAKFAELHIGWNTRPEEV 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  ELPQEALMVAEFARLVGAI+ TGSRPDSKWPQISRKTQLVMDAVKKSID  CK VY
Sbjct: 301 EVVNELPQEALMVAEFARLVGAIKTTGSRPDSKWPQISRKTQLVMDAVKKSIDSDCKPVY 360

BLAST of Moc08g35530 vs. NCBI nr
Match: XP_023528049.1 (uncharacterized oxidoreductase At4g09670-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633.6 bits (1633), Expect = 1.0e-177
Identity = 311/361 (86.15%), Postives = 335/361 (92.80%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M E PIRFGI+GCAEIARKVARAINSAPNS VSAVASRSLDKALNFAAVNGLP++VKVYG
Sbjct: 1   MAENPIRFGIVGCAEIARKVARAINSAPNSTVSAVASRSLDKALNFAAVNGLPENVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
            Y QILDDPCVDAVY+PLPTSLH+RWAILAA+KKKHILLEKPTAL+V ELD+ILDACE N
Sbjct: 61  GYGQILDDPCVDAVYMPLPTSLHQRWAILAAEKKKHILLEKPTALDVAELDKILDACELN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKE+ISD+ LFG+VNYIHSTSTTSGT++FLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEVISDTKLFGHVNYIHSTSTTSGTKQFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGR----TSAT 240
           LGALGDLAWYCIGA+LWAKDY+LPT V ALPDVTKNSAGVILSC+AS+ WG+    TSAT
Sbjct: 181 LGALGDLAWYCIGALLWAKDYKLPTQVHALPDVTKNSAGVILSCTASLQWGKTSNETSAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDIAISG+ GSL+VYDFIIPYQETSA F+F SGA FAELHIGWN RPEE+
Sbjct: 241 IHCSFLSHTSMDIAISGACGSLNVYDFIIPYQETSASFEFTSGAKFAELHIGWNTRPEEI 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  ELPQEALMVAEFARLVGAI+ TGSRPDSKWPQISRKTQLVMDAVKKSID  CK VY
Sbjct: 301 EVVNELPQEALMVAEFARLVGAIKTTGSRPDSKWPQISRKTQLVMDAVKKSIDNDCKPVY 360

BLAST of Moc08g35530 vs. ExPASy Swiss-Prot
Match: Q9SZ83 (Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g09670 PE=1 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.1e-113
Identity = 203/357 (56.86%), Postives = 261/357 (73.11%), Query Frame = 0

Query: 3   EAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYGSY 62
           E  IR G++GCA+IARKV+RAI+ APN+ +S VASRSL+KA  FA  N  P+S K++GSY
Sbjct: 4   ETQIRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSY 63

Query: 63  DQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGV 122
           + +L+DP +DA+Y+PLPTSLH  WAI AA+K KHILLEKP A+NV E D+I+DACE+NGV
Sbjct: 64  ESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGV 123

Query: 123 QFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDALG 182
           Q MDG+MW+H+PRTA +KE +SDS  FG +  + S  + +G  +FL+ +IRVKP LD LG
Sbjct: 124 QIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLG 183

Query: 183 ALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHW--GRTSATVHCS 242
           ALGD  WY I A L A ++ELP  V A P    N AGVILSC AS+ W  GRT AT++CS
Sbjct: 184 ALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRT-ATIYCS 243

Query: 243 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT 302
           FL++ +M+I   G++G+L V+DFIIPY+ET A F   + A F +L   W   P E  V T
Sbjct: 244 FLANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKT 303

Query: 303 ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 358
           ELPQEA MV EFARLVG I+  G++PD  WP ISRKTQLV+DAVK+S+D   + + L
Sbjct: 304 ELPQEACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of Moc08g35530 vs. ExPASy Swiss-Prot
Match: P49305 (Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 109/352 (30.97%), Postives = 166/352 (47.16%), Query Frame = 0

Query: 6   IRFGILGCAEIA-RKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYGSYDQ 65
           +RFGI+G A IA  KV  ++ SA    V A+ASR LD+A   A   G+  S   YGSYD+
Sbjct: 4   VRFGIIGTAAIAVEKVIPSMLSAEGLEVVAIASRDLDRARAAATRFGIGRS---YGSYDE 63

Query: 66  ILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGVQF 125
           IL DP ++AVY+PLP  LH  WAI AA+  KH+L EKP AL+V EL +++D  +  G + 
Sbjct: 64  ILADPEIEAVYIPLPNHLHVHWAIRAAEAGKHVLCEKPLALDVEELSRLIDCRDRTGRRI 123

Query: 126 MDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDAL--- 185
            +  M   HP+  ++ +I++   + G V  I           F E N+  K  ++     
Sbjct: 124 QEAVMIRAHPQWDEIFDIVASGEI-GEVRAIQGV--------FTEVNLDPKSIVNDASIG 183

Query: 186 -GALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCS 245
            GAL DL  Y I A       E P  V A+ D+        L+ +  +  G   AT+  S
Sbjct: 184 GGALYDLGVYPIAAARMVFAAE-PERVFAVSDLDPVFGIDRLTSAVLLFPGGRQATLVVS 243

Query: 246 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT 305
                  ++ I G+R S+S+ +   P  +        +G+  A             E   
Sbjct: 244 TQLALRHNVEIFGTRKSISLKNPFNPTPDDHCRIVLDNGSKLA---------AAAAETRR 303

Query: 306 ELPQEALMVAEFARLVGAIRATGSRP-DSKWPQISRKTQLVMDAVKKSIDGG 352
             P +   + +  R   AIR+    P + +W   S  T  V++A+++S + G
Sbjct: 304 VAPADQYRL-QAERFSAAIRSGSPLPIELEW---SLGTMKVLNAIQRSAERG 329

BLAST of Moc08g35530 vs. ExPASy Swiss-Prot
Match: D4GP30 (D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=HVO_B0029 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.4e-20
Identity = 67/187 (35.83%), Postives = 94/187 (50.27%), Query Frame = 0

Query: 8   FGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYGSYDQILD 67
           FGILG A I  K       A     +A+ASR   +A   A   G+P +   YGSY+ +L 
Sbjct: 3   FGILGTAGIGVKSVIPAVQASEHEAAAIASRDEARASAVADELGIPTA---YGSYEALLA 62

Query: 68  DPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGVQFMDG 127
           D  +DAVY+PLP  LH  W   AA +  H+L EKP   +  E   + D CE  GV  M+ 
Sbjct: 63  DDSLDAVYIPLPNGLHADWVRAAADRGLHVLCEKPLTASADETAAVFDYCEDAGVTLMEA 122

Query: 128 SMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDALGALGDL 187
            M+  HP T +  E+++     G V  +  TS  S       ++IR+ PDL A G++ D+
Sbjct: 123 FMYRFHPLTERAAELVASE--LGAV--VSVTSNFSFRLPDGADDIRIDPDL-AGGSVMDV 181

Query: 188 AWYCIGA 195
             Y + A
Sbjct: 183 GCYAVSA 181

BLAST of Moc08g35530 vs. ExPASy Swiss-Prot
Match: Q9ZA33 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces violaceoruber OX=1935 GN=gra-orf26 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.4e-19
Identity = 63/186 (33.87%), Postives = 100/186 (53.76%), Query Frame = 0

Query: 6   IRFGILGCAEIA-RKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYGSYDQ 65
           +R G+ GCA+IA R++  A  ++P++  +AVASRS +KA   A   G          YD 
Sbjct: 12  LRIGVAGCADIALRRMLPAFAASPHTEPTAVASRSSEKARAAAETFG----CAAVEGYDA 71

Query: 66  ILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGVQF 125
           +L+   VDAVY+PLP +LH  W   A +  KH+L EKP      +  ++LD     G+  
Sbjct: 72  LLERRDVDAVYIPLPVALHAPWTERALRAGKHVLAEKPLTARAADTARLLDLARERGLVL 131

Query: 126 MDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDALGAL 185
            +  +++HH     +++++ D+   G+V  + S S T   R    ++IR + DLD  GAL
Sbjct: 132 AENYLFVHHSAYTAVRDLV-DAGAIGDVRAL-SASFTIPPRS--ADDIRYRADLDG-GAL 188

Query: 186 GDLAWY 191
            D+  Y
Sbjct: 192 LDIGVY 188

BLAST of Moc08g35530 vs. ExPASy Swiss-Prot
Match: B3TMR8 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Actinomadura kijaniata OX=46161 GN=KijD10 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-18
Identity = 63/191 (32.98%), Postives = 101/191 (52.88%), Query Frame = 0

Query: 5   PIRFGILGCAEIA-RKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYGSYD 64
           PIR G++GCA+IA R+   A+ + P + V+A+ASR  D+A  F    G      V G Y 
Sbjct: 9   PIRVGVIGCADIAWRRALPALEAEPLTEVTAIASRRWDRAKRFTERFG---GEPVEG-YP 68

Query: 65  QILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGVQ 124
            +L+   VDAVY+PLP  LH  W   A +  KH+L EKP   +  + +++       G+ 
Sbjct: 69  ALLERDDVDAVYVPLPAVLHAEWIDRALRAGKHVLAEKPLTTDRPQAERLFAVARERGLL 128

Query: 125 FMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDALGA 184
            M+  M+LHHP+  ++ +++ +    G +  I S + +       + +IR + D+   GA
Sbjct: 129 LMENFMFLHHPQHRQVADMLDE----GVIGEIRSFAASFTIPPKPQGDIRYQADVGG-GA 188

Query: 185 LGDLAWYCIGA 195
           L D+  Y I A
Sbjct: 189 LLDIGVYPIRA 190

BLAST of Moc08g35530 vs. ExPASy TrEMBL
Match: A0A6J1CPV4 (uncharacterized oxidoreductase At4g09670-like OS=Momordica charantia OX=3673 GN=LOC111013201 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.3e-204
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG
Sbjct: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
           SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN
Sbjct: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCS 240
           LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCS
Sbjct: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTSATVHCS 240

Query: 241 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT 300
           FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT
Sbjct: 241 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT 300

Query: 301 ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 358
           ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL
Sbjct: 301 ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 357

BLAST of Moc08g35530 vs. ExPASy TrEMBL
Match: A0A6J1J8B2 (uncharacterized oxidoreductase At4g09670-like OS=Cucurbita maxima OX=3661 GN=LOC111482200 PE=4 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 1.5e-179
Identity = 315/361 (87.26%), Postives = 337/361 (93.35%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M E PIRFGI+GCAEIARKVARAINSAPNS VSAVASRSLDKALNFAAVNGLP++VKVYG
Sbjct: 1   MAENPIRFGIVGCAEIARKVARAINSAPNSTVSAVASRSLDKALNFAAVNGLPETVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
            YDQILDDPCVDAVY+PLPTSLH+RWAILAA+KKKHILLEKPTAL+V ELD+ILDACESN
Sbjct: 61  GYDQILDDPCVDAVYMPLPTSLHQRWAILAAEKKKHILLEKPTALDVEELDKILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKEIISD+ LFG+VNYIHSTSTTSGT++FLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEIISDTKLFGHVNYIHSTSTTSGTKQFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGR----TSAT 240
           LGALGDLAWYCIGA+LWAKDY+LPT V ALPDVTKNSAGVILSC+AS+ WG+    TSAT
Sbjct: 181 LGALGDLAWYCIGALLWAKDYKLPTQVHALPDVTKNSAGVILSCTASLQWGKSSNETSAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDIAISG+ GSL+VYDFIIPYQETSA F+F SGA FAELHIGWN RPEEV
Sbjct: 241 IHCSFLSHTSMDIAISGACGSLNVYDFIIPYQETSASFEFTSGAKFAELHIGWNTRPEEV 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  ELPQEALMVAEFARLVGAI+ TGSRPDSKWPQISRKTQLVMDAVKKSID  CK VY
Sbjct: 301 EVVNELPQEALMVAEFARLVGAIKTTGSRPDSKWPQISRKTQLVMDAVKKSIDSDCKPVY 360

BLAST of Moc08g35530 vs. ExPASy TrEMBL
Match: A0A6J1F4U1 (uncharacterized oxidoreductase At4g09670-like OS=Cucurbita moschata OX=3662 GN=LOC111442125 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 9.8e-179
Identity = 314/361 (86.98%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M   PIRFGI+GCAEIARKVARAINSAPNS VSAVASRSLDKALNFAAVNGLPD+VKVYG
Sbjct: 1   MAGNPIRFGIVGCAEIARKVARAINSAPNSTVSAVASRSLDKALNFAAVNGLPDTVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
            YDQILDDPCVDAVY+PLPTSLH+RWAILAA+KKKHILLEKPTAL+V ELD+ILDACESN
Sbjct: 61  GYDQILDDPCVDAVYMPLPTSLHQRWAILAAEKKKHILLEKPTALDVEELDKILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKMKEIISD+ LFG+VNYIHSTSTTSGT++FLEENIRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMKEIISDTKLFGHVNYIHSTSTTSGTKQFLEENIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGRTS----AT 240
           LGALGDLAWYCIGA+LWAKDY+LPT V ALPDVTKNSAGVILSC+AS+ WG++S    AT
Sbjct: 181 LGALGDLAWYCIGALLWAKDYKLPTQVHALPDVTKNSAGVILSCTASLQWGKSSNETFAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDIAISG+ GSL+VYDFIIPYQETSA F+F SGA FAELHIGWN RPEEV
Sbjct: 241 IHCSFLSHTSMDIAISGACGSLNVYDFIIPYQETSASFEFTSGAKFAELHIGWNTRPEEV 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  ELPQEALMVAEFARLVGAI+ TGSRPDSKWPQISRKTQLVMDAVKKSID  CK VY
Sbjct: 301 EVVNELPQEALMVAEFARLVGAIKTTGSRPDSKWPQISRKTQLVMDAVKKSIDSDCKPVY 360

BLAST of Moc08g35530 vs. ExPASy TrEMBL
Match: A0A5D3CUF0 (Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G00170 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.0e-171
Identity = 299/361 (82.83%), Postives = 328/361 (90.86%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M E PIRFGI+GCAEIARKVARAINSAPNSAV+AVASRSLDKALNFAAVNGLP++VKVYG
Sbjct: 1   MAENPIRFGIVGCAEIARKVARAINSAPNSAVTAVASRSLDKALNFAAVNGLPETVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
           SY+QILDDPCVDAVY+PLPTSLHRRWAILAA+KKKHILLEKPTAL+V ELDQILDACESN
Sbjct: 61  SYNQILDDPCVDAVYMPLPTSLHRRWAILAAEKKKHILLEKPTALDVEELDQILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKM+EIISD  LFG VNYIHS+STTS T++FLEE+IRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMEEIISDPKLFGYVNYIHSSSTTSATKQFLEEDIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGR----TSAT 240
           LGALGDLAWYCIGA+LWAK+YELP  V ALPDVTKNSAGVILSC+AS+HWG+    T+AT
Sbjct: 181 LGALGDLAWYCIGALLWAKNYELPIQVRALPDVTKNSAGVILSCTASLHWGKSSKETTAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDI I+G+ GS++VYDFIIPYQETSA FD   GA FAELHIGWN +PEEV
Sbjct: 241 IHCSFLSHTSMDITITGTHGSVNVYDFIIPYQETSASFDITLGAKFAELHIGWNTKPEEV 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  E+PQE LM+AEF+RLV  IR TG  PDSKWPQISRKTQLVMDAVKKSID  C+ VY
Sbjct: 301 EVANEIPQEVLMIAEFSRLVQVIRTTGCCPDSKWPQISRKTQLVMDAVKKSIDTDCRPVY 360

BLAST of Moc08g35530 vs. ExPASy TrEMBL
Match: A0A1S3C2A0 (uncharacterized oxidoreductase At4g09670-like OS=Cucumis melo OX=3656 GN=LOC103496207 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.0e-171
Identity = 299/361 (82.83%), Postives = 328/361 (90.86%), Query Frame = 0

Query: 1   MGEAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYG 60
           M E PIRFGI+GCAEIARKVARAINSAPNSAV+AVASRSLDKALNFAAVNGLP++VKVYG
Sbjct: 1   MAENPIRFGIVGCAEIARKVARAINSAPNSAVTAVASRSLDKALNFAAVNGLPETVKVYG 60

Query: 61  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 120
           SY+QILDDPCVDAVY+PLPTSLHRRWAILAA+KKKHILLEKPTAL+V ELDQILDACESN
Sbjct: 61  SYNQILDDPCVDAVYMPLPTSLHRRWAILAAEKKKHILLEKPTALDVEELDQILDACESN 120

Query: 121 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 180
           GVQFMDGSMWLHHPRTAKM+EIISD  LFG VNYIHS+STTS T++FLEE+IRVKPDLDA
Sbjct: 121 GVQFMDGSMWLHHPRTAKMEEIISDPKLFGYVNYIHSSSTTSATKQFLEEDIRVKPDLDA 180

Query: 181 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHWGR----TSAT 240
           LGALGDLAWYCIGA+LWAK+YELP  V ALPDVTKNSAGVILSC+AS+HWG+    T+AT
Sbjct: 181 LGALGDLAWYCIGALLWAKNYELPIQVRALPDVTKNSAGVILSCTASLHWGKSSKETTAT 240

Query: 241 VHCSFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEV 300
           +HCSFLSHTSMDI I+G+ GS++VYDFIIPYQETSA FD   GA FAELHIGWN +PEEV
Sbjct: 241 IHCSFLSHTSMDITITGTHGSVNVYDFIIPYQETSASFDITLGAKFAELHIGWNTKPEEV 300

Query: 301 EVGTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVY 358
           EV  E+PQE LM+AEF+RLV  IR TG  PDSKWPQISRKTQLVMDAVKKSID  C+ VY
Sbjct: 301 EVANEIPQEVLMIAEFSRLVQVIRTTGCCPDSKWPQISRKTQLVMDAVKKSIDTDCRPVY 360

BLAST of Moc08g35530 vs. TAIR 10
Match: AT4G09670.1 (Oxidoreductase family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.2e-114
Identity = 203/357 (56.86%), Postives = 261/357 (73.11%), Query Frame = 0

Query: 3   EAPIRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGLPDSVKVYGSY 62
           E  IR G++GCA+IARKV+RAI+ APN+ +S VASRSL+KA  FA  N  P+S K++GSY
Sbjct: 4   ETQIRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSY 63

Query: 63  DQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGV 122
           + +L+DP +DA+Y+PLPTSLH  WAI AA+K KHILLEKP A+NV E D+I+DACE+NGV
Sbjct: 64  ESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGV 123

Query: 123 QFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDALG 182
           Q MDG+MW+H+PRTA +KE +SDS  FG +  + S  + +G  +FL+ +IRVKP LD LG
Sbjct: 124 QIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLG 183

Query: 183 ALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHW--GRTSATVHCS 242
           ALGD  WY I A L A ++ELP  V A P    N AGVILSC AS+ W  GRT AT++CS
Sbjct: 184 ALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRT-ATIYCS 243

Query: 243 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVGT 302
           FL++ +M+I   G++G+L V+DFIIPY+ET A F   + A F +L   W   P E  V T
Sbjct: 244 FLANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKT 303

Query: 303 ELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 358
           ELPQEA MV EFARLVG I+  G++PD  WP ISRKTQLV+DAVK+S+D   + + L
Sbjct: 304 ELPQEACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of Moc08g35530 vs. TAIR 10
Match: AT1G66130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 4.0e-100
Identity = 179/360 (49.72%), Postives = 248/360 (68.89%), Query Frame = 0

Query: 6   IRFGILGCAEIARKVARAINSAPNSAVSAVASRSLDKALNFAAVNGL-PDSVKVYGSYDQ 65
           +RFG+LGC   A K  R +  + N+ + A++  SL+ A  FA  N L P++V +YGSY++
Sbjct: 7   VRFGLLGCIRFASKFVRTVTESSNAVIIAISDPSLETAKTFATGNNLSPETVTIYGSYEE 66

Query: 66  ILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESNGVQF 125
           +L+D  VDAVYL +P +   RWA+ AA+KKKH+L+EKP A +  EL++I++ACE NGVQF
Sbjct: 67  LLNDANVDAVYLTMPVTQRARWAVTAAEKKKHVLVEKPPAQDATELEKIVEACEYNGVQF 126

Query: 126 MDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDALGAL 185
           MDG++WLHH RT K+++ + DS L G+V +++ST TT    + LE     K  +   GA+
Sbjct: 127 MDGTIWLHHQRTVKIRDTMFDSGLLGDVRHMYSTMTTPVPEQVLER--LTKEAMGLAGAI 186

Query: 186 GDLAWYCIGAILWAKDYELPTHVCALP-DVTKNSAGVILSCSASIHWGRTSAT---VHCS 245
           G+L WY IGA LWA  Y++P  V ALP  V+ NS G ILSC+AS+ +G T+ T   VHCS
Sbjct: 187 GELGWYPIGAALWAMSYQMPISVRALPSSVSTNSVGTILSCTASLQFGSTATTTAIVHCS 246

Query: 246 FLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEV-- 305
           FLS  S D+AISGS+GS+ + D++IPY+E  A F++ SGA F ++HIGWN  PE V V  
Sbjct: 247 FLSQLSTDLAISGSKGSIQMNDYVIPYKEDKAWFEYTSGAKFVDMHIGWNVTPERVTVDC 306

Query: 306 -GTELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 358
            GT   QEA+M+ EF RLV  I+      D +WP IS+KTQL++DAVKKS+D GC+ V+L
Sbjct: 307 SGTPETQEAMMLREFTRLVEGIKRGDLEADRRWPDISKKTQLIVDAVKKSVDIGCEVVHL 364

BLAST of Moc08g35530 vs. TAIR 10
Match: AT1G34200.1 (Glyceraldehyde-3-phosphate dehydrogenase-like family protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.7e-96
Identity = 176/358 (49.16%), Postives = 241/358 (67.32%), Query Frame = 0

Query: 2   GEAPIRFGILGCAEIARKVARAINSAPNSAVSAVA-SRSLDKALNFAAVNGLPDSVKVYG 61
           G+  IR G+L C    RK++RAIN +PN+ +SA+A + S+++A +FA  N  P + K++ 
Sbjct: 3   GDNQIRIGLLSCTNKVRKLSRAINLSPNATISAIATTSSIEEAKSFAKSNNFPPNTKLHS 62

Query: 62  SYDQILDDPCVDAVYLPLPTSLHRRWAILAAQKKKHILLEKPTALNVGELDQILDACESN 121
           SY+ +L+DP VDAVY P+PT LH  WA LAA K KHILL+KP ALNV E DQI++ACE N
Sbjct: 63  SYESLLEDPDVDAVYFPIPTRLHVEWATLAAIKGKHILLDKPVALNVAEFDQIVEACEVN 122

Query: 122 GVQFMDGSMWLHHPRTAKMKEIISDSNLFGNVNYIHSTSTTSGTREFLEENIRVKPDLDA 181
           GVQFMDG+ W+H PRT K+KE ++D   FG +  ++S  + + + +FL+ +IRVKP LD 
Sbjct: 123 GVQFMDGTQWMHSPRTDKIKEFVNDLESFGQIKSVYSCFSFAASDDFLKHDIRVKPGLDG 182

Query: 182 LGALGDLAWYCIGAILWAKDYELPTHVCALPDVTKNSAGVILSCSASIHW-GRTSATVHC 241
           LGALGD  WY I AIL   +++LP  V AL     N AG++LSC A   W    +AT++C
Sbjct: 183 LGALGDAGWYTIQAILLVNNFKLPKTVTALSGSVLNDAGIVLSCGALFDWEDGVNATIYC 242

Query: 242 SFLSHTSMDIAISGSRGSLSVYDFIIPYQETSALFDFISGAAFAELHIGWNKRPEEVEVG 301
           SFL++ +M+I   G++GSL V+DF+IPY ET A+F    G   +           E +V 
Sbjct: 243 SFLANLTMEITAIGTKGSLRVHDFVIPYMETEAVFTTSGGGCVS-----------EHKVM 302

Query: 302 TELPQEALMVAEFARLVGAIRATGSRPDSKWPQISRKTQLVMDAVKKSIDGGCKTVYL 358
           TEL QEA MV EFARLVG I+  G++PD  W + SR TQL++DAVK+SID   + V+L
Sbjct: 303 TELSQEACMVMEFARLVGEIKNRGAKPDGFWMRFSRNTQLLVDAVKESIDNNLEPVFL 349

BLAST of Moc08g35530 vs. TAIR 10
Match: AT4G17370.1 (Oxidoreductase family protein )

HSP 1 Score: 45.8 bits (107), Expect = 8.3e-05
Identity = 26/103 (25.24%), Postives = 49/103 (47.57%), Query Frame = 0

Query: 56  VKVYGSYDQILDDPCVDAVYLPLPTSLHRR--WAILAAQKKKHILLEKPTALNVGELDQI 115
           +KV+  ++++L     D + +  P   H +    I+   K  H+L+EKP    V +  Q+
Sbjct: 63  LKVFSGHEELLKSELCDVIVVSSPNMTHHQILMDIINYSKPHHVLVEKPLCTTVADCKQV 122

Query: 116 LDACESNGVQFMD-GSMWLHHPRTAKMKEIISDSNLFGNVNYI 156
           L+A +      +  G  + + P  AK+ E +   + FGNV  +
Sbjct: 123 LEAAKKRSDMVVQVGLEYRYMPPVAKLIEQVKGRD-FGNVKMV 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143296.14.8e-204100.00uncharacterized oxidoreductase At4g09670-like [Momordica charantia][more]
KAG7017970.13.1e-17987.26putative oxidoreductase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022983648.13.1e-17987.26uncharacterized oxidoreductase At4g09670-like [Cucurbita maxima][more]
XP_022935172.12.0e-17886.98uncharacterized oxidoreductase At4g09670-like [Cucurbita moschata][more]
XP_023528049.11.0e-17786.15uncharacterized oxidoreductase At4g09670-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SZ833.1e-11356.86Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
P493051.1e-2530.97Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1[more]
D4GP301.4e-2035.83D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / ... [more]
Q9ZA334.4e-1933.87dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces viola... [more]
B3TMR81.7e-1832.98dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Actinomadura kijan... [more]
Match NameE-valueIdentityDescription
A0A6J1CPV42.3e-204100.00uncharacterized oxidoreductase At4g09670-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1J8B21.5e-17987.26uncharacterized oxidoreductase At4g09670-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1F4U19.8e-17986.98uncharacterized oxidoreductase At4g09670-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A5D3CUF02.0e-17182.83Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3C2A02.0e-17182.83uncharacterized oxidoreductase At4g09670-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT4G09670.12.2e-11456.86Oxidoreductase family protein [more]
AT1G66130.14.0e-10049.72NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G34200.12.7e-9649.16Glyceraldehyde-3-phosphate dehydrogenase-like family protein [more]
AT4G17370.18.3e-0525.24Oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.360.10Dihydrodipicolinate Reductase; domain 2coord: 133..357
e-value: 5.8E-62
score: 211.2
NoneNo IPR availableGENE3D3.40.50.720coord: 2..132
e-value: 1.9E-35
score: 124.1
NoneNo IPR availablePANTHERPTHR46368:SF5NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 1..357
NoneNo IPR availablePANTHERPTHR46368FAMILY NOT NAMEDcoord: 1..357
NoneNo IPR availableSUPERFAMILY55347Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domaincoord: 133..316
IPR000683Gfo/Idh/MocA-like oxidoreductase, N-terminalPFAMPF01408GFO_IDH_MocAcoord: 6..125
e-value: 9.2E-19
score: 68.4
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 5..157

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g35530.1Moc08g35530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000166 nucleotide binding