Moc08g34910 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g34910
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	pectinesterase inhibitor-like
Location	chr8: 25528376 .. 25528924 (-)
RNA-Seq Expression	Moc08g34910
Synteny	Moc08g34910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGCGAATATTTCAGGTGCGATGTTGGTGGTGGTTTGGCTGAGCGTGGCGGCGGCGGCGGAGGCGATCAGGCCGGGGAAGCCGGACATGGGCCTAATCGAACAAACGTGCAGCCAAACGCAGCACCCGGGGCTGTGCCTGAGCTCGCTGAAGTCGGACCCGAACAGCTTCCCGGCGGACAAGACGGGGCTGGCGCTGGTGGCGCTGCGGCTGGTGTCGTCCAACGCGTCCGACATATCGGAAGGGATCAAGGTGATGCTGAACGACACTCGGCTGGCGGACCAGCCCGGGGTCCAGCAGGGCCTCTACGATTGCCTCGACGAGTACCTGGACGCGTCACAGCAGCTCGACGACTCCATCGCCGCCCTCCTCGTCAAGGCCTACGGTGACGTGGACAAGTGGGTCAACGCCGCCGTCGCCGCCGTCCGCACGTGCGAGGCCTCCTTCAGCGTCAAGCCCTCCGTCCTCACCCCGAGGAACGACAACTTCATTAAGCTCTGTCAGAACGCCTTGACCATCGCCCACATTGCTGAAACTGAAAATTAA

mRNA sequence

ATGAAGGCGAATATTTCAGGTGCGATGTTGGTGGTGGTTTGGCTGAGCGTGGCGGCGGCGGCGGAGGCGATCAGGCCGGGGAAGCCGGACATGGGCCTAATCGAACAAACGTGCAGCCAAACGCAGCACCCGGGGCTGTGCCTGAGCTCGCTGAAGTCGGACCCGAACAGCTTCCCGGCGGACAAGACGGGGCTGGCGCTGGTGGCGCTGCGGCTGGTGTCGTCCAACGCGTCCGACATATCGGAAGGGATCAAGGTGATGCTGAACGACACTCGGCTGGCGGACCAGCCCGGGGTCCAGCAGGGCCTCTACGATTGCCTCGACGAGTACCTGGACGCGTCACAGCAGCTCGACGACTCCATCGCCGCCCTCCTCGTCAAGGCCTACGGTGACGTGGACAAGTGGGTCAACGCCGCCGTCGCCGCCGTCCGCACGTGCGAGGCCTCCTTCAGCGTCAAGCCCTCCGTCCTCACCCCGAGGAACGACAACTTCATTAAGCTCTGTCAGAACGCCTTGACCATCGCCCACATTGCTGAAACTGAAAATTAA

Coding sequence (CDS)

ATGAAGGCGAATATTTCAGGTGCGATGTTGGTGGTGGTTTGGCTGAGCGTGGCGGCGGCGGCGGAGGCGATCAGGCCGGGGAAGCCGGACATGGGCCTAATCGAACAAACGTGCAGCCAAACGCAGCACCCGGGGCTGTGCCTGAGCTCGCTGAAGTCGGACCCGAACAGCTTCCCGGCGGACAAGACGGGGCTGGCGCTGGTGGCGCTGCGGCTGGTGTCGTCCAACGCGTCCGACATATCGGAAGGGATCAAGGTGATGCTGAACGACACTCGGCTGGCGGACCAGCCCGGGGTCCAGCAGGGCCTCTACGATTGCCTCGACGAGTACCTGGACGCGTCACAGCAGCTCGACGACTCCATCGCCGCCCTCCTCGTCAAGGCCTACGGTGACGTGGACAAGTGGGTCAACGCCGCCGTCGCCGCCGTCCGCACGTGCGAGGCCTCCTTCAGCGTCAAGCCCTCCGTCCTCACCCCGAGGAACGACAACTTCATTAAGCTCTGTCAGAACGCCTTGACCATCGCCCACATTGCTGAAACTGAAAATTAA

Protein sequence

MKANISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAETEN

Homology

[Close]

BLAST of Moc08g34910 vs. NCBI nr
Match: XP_022155314.1 (pectinesterase inhibitor-like [Momordica charantia])

HSP 1 Score: 355.9 bits (912), Expect = 2.1e-94
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MKANISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPA 60
           MKANISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPA
Sbjct: 1   MKANISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPA 60

Query: 61  DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDS 120
           DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDS
Sbjct: 61  DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDS 120

Query: 121 IAALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 180
           IAALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET
Sbjct: 121 IAALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 180

Query: 181 EN 183
           EN
Sbjct: 181 EN 182

[Close]

BLAST of Moc08g34910 vs. NCBI nr
Match: XP_038906317.1 (cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida])

HSP 1 Score: 219.9 bits (559), Expect = 1.8e-53
Identity = 111/176 (63.07%), Postives = 139/176 (78.98%), Query Frame = 0


Query: 5   ISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTG 64
           +S  + V+    VA   +A  P +  + +I+  C+QT +  LC+ SL SDPNSFPADK G
Sbjct: 4   VSAILFVLCLSMVAQRLDAASPAEDGLSMIDHMCAQTNYKDLCVISLTSDPNSFPADKMG 63

Query: 65  LALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAAL 124
           LALVALR  SSNASDISE IKVMLNDT L ++P VQQGL+DCLDEYLDASQQLDD+IAA+
Sbjct: 64  LALVALRFASSNASDISESIKVMLNDTSL-NKPSVQQGLFDCLDEYLDASQQLDDAIAAI 123

Query: 125 LVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 181
           LVKAYGDV+KWV+AA+A+VRTCE  F+ KPS+L P+ND FIKLC+ AL+I+ +A+T
Sbjct: 124 LVKAYGDVEKWVDAAIASVRTCENEFATKPSLLAPKNDVFIKLCEIALSISKVADT 178

[Close]

BLAST of Moc08g34910 vs. NCBI nr
Match: KAA0044390.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK29518.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 215.7 bits (548), Expect = 3.3e-52
Identity = 111/157 (70.70%), Postives = 129/157 (82.17%), Query Frame = 0


Query: 23  AIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISE 82
           A +  K  +  IE+ C+QT +  LC++SL SDPNSFPADK GLALVALRL SSNASDISE
Sbjct: 9   ASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLASSNASDISE 68

Query: 83  GIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAA 142
            IKVMLN+T   ++P VQQGL+DCLDEYLDASQQLDDSIAA++ KAYGDV+KWV+AAVA 
Sbjct: 69  SIKVMLNETS-QNEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEKWVHAAVAD 128

Query: 143 VRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAE 180
           VRTCE SF  KPSVLTPRN+ FIKLC  AL+I+ IAE
Sbjct: 129 VRTCENSFPTKPSVLTPRNEEFIKLCDIALSISRIAE 164

[Close]

BLAST of Moc08g34910 vs. NCBI nr
Match: XP_023528768.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.9 bits (546), Expect = 5.7e-52
Identity = 119/183 (65.03%), Postives = 141/183 (77.05%), Query Frame = 0


Query: 1   MKANISGAMLVVVWLSVAAAA-EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFP 60
           M+ N SGA++VV+ L +AA   +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+P
Sbjct: 1   MRGNQSGAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYP 60

Query: 61  ADKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDD 120
           ADKTGLALVALR  SSNASDISE IKVM ND+ LA +P VQ+ L DCLDEYLDASQQLDD
Sbjct: 61  ADKTGLALVALRFASSNASDISESIKVMFNDSTLA-KPMVQEALLDCLDEYLDASQQLDD 120

Query: 121 SIAALLVKAYGDVDKWVNAAVAAVRTCEASF-SVKPSVLTPRNDNFIKLCQNALTIAHIA 180
           SIAAL VKAYGD++KWVN A+  VRTC     S +P +LT RND FIKLC+ ALTI+ IA
Sbjct: 121 SIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIA 180

Query: 181 ETE 182
           + E
Sbjct: 181 QGE 182

[Close]

BLAST of Moc08g34910 vs. NCBI nr
Match: XP_004152360.2 (cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus] >KGN52826.1 hypothetical protein Csa_015223 [Cucumis sativus])

HSP 1 Score: 213.0 bits (541), Expect = 2.1e-51
Identity = 114/178 (64.04%), Postives = 137/178 (76.97%), Query Frame = 0


Query: 8   AMLVVVWLSVA-----AAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADK 67
           A+L+V+ L +A     AA  A   G   +G+I++ C+QT +  LC++SL SDPNSFPADK
Sbjct: 6   AILLVLCLCMAPQRLDAAGAAQEEG---LGMIQKMCAQTNYKDLCITSLTSDPNSFPADK 65

Query: 68  TGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIA 127
            GLALVALRL SSNASDISE IKVMLN+T   ++P VQQ L+DCLDEYL+ASQQLDDSIA
Sbjct: 66  MGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIA 125

Query: 128 ALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 181
           A++ KAYGDV +WV  AV  VRTCE+SF  KPSVLTPRN+ FIKLC  AL+I  IAET
Sbjct: 126 AIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180

[Close]

BLAST of Moc08g34910 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.8e-08
Identity = 44/152 (28.95%), Postives = 71/152 (46.71%), Query Frame = 0


Query: 33  LIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISEGIKVMLNDT- 92
           L+  TC+ T +  +C+S+L +DP+S  AD  GL+ +A+ + ++NAS  ++    + N T 
Sbjct: 61  LVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALANGTA 120

Query: 93  ----RLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRTCE 152
                 A    VQ  L  C  +Y DA   L  +  ++  + Y      V+A     + C+
Sbjct: 121 PLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYPQVCK 180

Query: 153 ASF-----SVKPSVLTPRNDNFIKLCQNALTI 175
           A F        P+ L  R +   KLC  AL I
Sbjct: 181 ALFRRQRPGAYPAELAAREEALNKLCSVALDI 212

[Close]

BLAST of Moc08g34910 vs. ExPASy TrEMBL
Match: A0A6J1DPW6 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 9.9e-95
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MKANISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPA 60
           MKANISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPA
Sbjct: 1   MKANISGAMLVVVWLSVAAAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPA 60

Query: 61  DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDS 120
           DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDS
Sbjct: 61  DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDS 120

Query: 121 IAALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 180
           IAALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET
Sbjct: 121 IAALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 180

Query: 181 EN 183
           EN
Sbjct: 181 EN 182

[Close]

BLAST of Moc08g34910 vs. ExPASy TrEMBL
Match: A0A5D3E196 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001210 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.6e-52
Identity = 111/157 (70.70%), Postives = 129/157 (82.17%), Query Frame = 0


Query: 23  AIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISE 82
           A +  K  +  IE+ C+QT +  LC++SL SDPNSFPADK GLALVALRL SSNASDISE
Sbjct: 9   ASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLASSNASDISE 68

Query: 83  GIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAA 142
            IKVMLN+T   ++P VQQGL+DCLDEYLDASQQLDDSIAA++ KAYGDV+KWV+AAVA 
Sbjct: 69  SIKVMLNETS-QNEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEKWVHAAVAD 128

Query: 143 VRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAE 180
           VRTCE SF  KPSVLTPRN+ FIKLC  AL+I+ IAE
Sbjct: 129 VRTCENSFPTKPSVLTPRNEEFIKLCDIALSISRIAE 164

[Close]

BLAST of Moc08g34910 vs. ExPASy TrEMBL
Match: A0A0A0KY96 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 1.0e-51
Identity = 114/178 (64.04%), Postives = 137/178 (76.97%), Query Frame = 0


Query: 8   AMLVVVWLSVA-----AAAEAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADK 67
           A+L+V+ L +A     AA  A   G   +G+I++ C+QT +  LC++SL SDPNSFPADK
Sbjct: 6   AILLVLCLCMAPQRLDAAGAAQEEG---LGMIQKMCAQTNYKDLCITSLTSDPNSFPADK 65

Query: 68  TGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIA 127
            GLALVALRL SSNASDISE IKVMLN+T   ++P VQQ L+DCLDEYL+ASQQLDDSIA
Sbjct: 66  MGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDSIA 125

Query: 128 ALLVKAYGDVDKWVNAAVAAVRTCEASFSVKPSVLTPRNDNFIKLCQNALTIAHIAET 181
           A++ KAYGDV +WV  AV  VRTCE+SF  KPSVLTPRN+ FIKLC  AL+I  IAET
Sbjct: 126 AIIAKAYGDVQEWVRVAVTNVRTCESSFPTKPSVLTPRNEEFIKLCDIALSITKIAET 180

[Close]

BLAST of Moc08g34910 vs. ExPASy TrEMBL
Match: A0A6J1F7F2 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.7e-49
Identity = 113/176 (64.20%), Postives = 136/176 (77.27%), Query Frame = 0


Query: 8   AMLVVVWLSVAAAA-EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLA 67
           A++VV+ L +AA   +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+PADKTGLA
Sbjct: 6   AIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 65

Query: 68  LVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLV 127
           LVALR  SSNASDISE IKVM ND+ L+ +P VQ+ L DCLDEYLDASQQLDDSIAAL V
Sbjct: 66  LVALRFASSNASDISESIKVMFNDSTLS-KPMVQEALLDCLDEYLDASQQLDDSIAALTV 125

Query: 128 KAYGDVDKWVNAAVAAVRTCEASF-SVKPSVLTPRNDNFIKLCQNALTIAHIAETE 182
           KAYGD++KWVN A+  VRTC     S +P +LT RN+ FIKLC+ ALTI+ IA+ E
Sbjct: 126 KAYGDLEKWVNIAITDVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQGE 180

[Close]

BLAST of Moc08g34910 vs. ExPASy TrEMBL
Match: A0A6J1J9E7 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 6.3e-49
Identity = 112/176 (63.64%), Postives = 135/176 (76.70%), Query Frame = 0


Query: 8   AMLVVVWLSVAAAA-EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLA 67
           A++V++ L +A    +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+PADKTGLA
Sbjct: 6   AIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 65

Query: 68  LVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLV 127
           LVALR  SSNASDISE IKVM ND+ L+ +P VQ+ L DCLDEYLDASQQLDDSIAAL V
Sbjct: 66  LVALRFASSNASDISESIKVMFNDSTLS-KPMVQEALLDCLDEYLDASQQLDDSIAALTV 125

Query: 128 KAYGDVDKWVNAAVAAVRTCEASF-SVKPSVLTPRNDNFIKLCQNALTIAHIAETE 182
           KAYGD++KWVN A+  VRTC     S +P +LT RND FIKLC+ ALTI+ IA+ E
Sbjct: 126 KAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQGE 180

[Close]

BLAST of Moc08g34910 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 6.9e-24
Identity = 59/153 (38.56%), Postives = 92/153 (60.13%), Query Frame = 0


Query: 27  GKPDMGLIEQTCSQTQHPGLCLSSLKSDPNS--FPADKTGLALVALRLVSSNASDISEGI 86
           G  + G+I   C  +  P LCLSS+ S P S  F      + ++A+   S+NAS  S  I
Sbjct: 20  GNANSGMISDLCKHSDDPNLCLSSITSRPESGEFAGTSNQIEIIAISAASANASATSSYI 79

Query: 87  KVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVR 146
           K  L++  L  +P ++  L DC  +Y DA +QLDDSI+A+L  A+ DVD W++AA++A+ 
Sbjct: 80  KQKLSNEDL--EPAIEDTLEDCQKDYQDAVEQLDDSISAMLADAHTDVDVWLSAAISAIE 139

Query: 147 TCEASFSVK---PSVLTPRNDNFIKLCQNALTI 175
           +C ++   +    + L+ RN+ F+KLC+NAL I
Sbjct: 140 SCGSALGSRAGNDAELSQRNEVFLKLCKNALMI 170

[Close]

BLAST of Moc08g34910 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 91.7 bits (226), Expect = 6.7e-19
Identity = 54/131 (41.22%), Postives = 77/131 (58.78%), Query Frame = 0


Query: 47  CLSSLKSDPNSFPADKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDC 106
           C++SL S P +  A    L ++AL + SSNASD S  IK  L    L  +P ++  L DC
Sbjct: 26  CVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNL--EPALEDTLDDC 85

Query: 107 LDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRTCEASFSVK---PSVLTPRNDN 166
              YLDA  QLDDS+AAL+  ++ DVD W+N A++    CE + + +    + L  RN N
Sbjct: 86  SKNYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTN 145

Query: 167 FIKLCQNALTI 175
            +KLC++AL I
Sbjct: 146 LLKLCKDALLI 154

[Close]

BLAST of Moc08g34910 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.9e-06
Identity = 33/125 (26.40%), Postives = 64/125 (51.20%), Query Frame = 0


Query: 46  LCLSSLKSDPNSFPA-DKTGLALVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLY 105
           LC++SL+++P +  A D  GL + + +   + A+ +   +  ++   ++     +   L 
Sbjct: 42  LCVTSLETNPQAKTAKDLAGLVMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMP--LR 101

Query: 106 DCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRTCEASFSVK--PSVLTPRND 165
           DCL  Y DA   L++++A +  + Y  V   ++AA+    TCE  F  +  PS +T  ND
Sbjct: 102 DCLQLYTDAIGSLNEALAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKAPSPVTKEND 161

Query: 166 NFIKL 168
           N  ++
Sbjct: 162 NLYQM 164

[Close]

BLAST of Moc08g34910 vs. TAIR 10
Match: AT3G12880.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 8.5e-06
Identity = 42/147 (28.57%), Postives = 65/147 (44.22%), Query Frame = 0


Query: 33  LIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLALVALRLVSSNASDISEGIKVMLNDTR 92
           L+ Q C  + +P LCL  L SDP S  ADK GLA + L+ ++S+   +++    +   ++
Sbjct: 31  LMMQQCHNSDNPTLCLRCLSSDPGSHEADKVGLARIILKCINSHLLVLTKNASTL--GSQ 90

Query: 93  LADQPGVQQGLYDCLDEYLDASQQLDDSIAALLV----KAYGDVD-KWVNAAVAAVRTCE 152
               P     L  C   +  A   + ++   L+     KA  DV  K VN  V+   + E
Sbjct: 91  HYQNPNTAAALKQCGLGFSTAKHGVGEADTQLITGDYDKAAYDVSVKVVNPPVSCRNSLE 150

Query: 153 ASFSVKPSVLTPRNDNFIKLCQNALTI 175
           A     PS      D ++ L Q  L I
Sbjct: 151 ALNIQVPSSFRYHMDVYLALTQALLRI 175

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BLAST of Moc08g34910 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.5e-05
Identity = 42/141 (29.79%), Postives = 69/141 (48.94%), Query Frame = 0


Query: 33  LIEQTCSQ--TQHPGL----CLSSLKSDPNSFPADKTGLALVALRLVSS-NASDISEGIK 92
           LI  +C +  T+ P L    C+ SL+ +P S    KT  +L  L  VS+ NA   +  +K
Sbjct: 23  LIRDSCKKATTKDPKLKYDFCVKSLEENPQS----KTAKSLEGLVFVSTKNAVSKTTSLK 82

Query: 93  VMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLVKAYGDVDKWVNAAVAAVRT 152
            M++     D+  V++ L DCL+ Y +A   L+ S+  +  + Y      ++AA+ A  +
Sbjct: 83  GMVDKILKEDKYEVERPLLDCLELYTEAIDSLNQSLDTVKSRDYKTATMLMSAAMDAPGS 142

Query: 153 CEASFSVK----PSVLTPRND 163
           CE  F+ +     S  T  ND
Sbjct: 143 CETKFTKRKKAVKSPFTKEND 159

The following BLAST results are available for this feature:

BLAST of Moc08g34910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155314.1	2.1e-94	100.00	pectinesterase inhibitor-like [Momordica charantia]	[more]
XP_038906317.1	1.8e-53	63.07	cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida]	[more]
KAA0044390.1	3.3e-52	70.70	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK29518.1 putative inv...	[more]
XP_023528768.1	5.7e-52	65.03	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_004152360.2	2.1e-51	64.04	cell wall / vacuolar inhibitor of fructosidase 2 [Cucumis sativus] >KGN52826.1 h...	[more]

BLAST of Moc08g34910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0J8J8	6.8e-08	28.95	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of Moc08g34910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPW6	9.9e-95	100.00	pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=...	[more]
A0A5D3E196	1.6e-52	70.70	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A0A0KY96	1.0e-51	64.04	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 S...	[more]
A0A6J1F7F2	1.7e-49	64.20	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4...	[more]
A0A6J1J9E7	6.3e-49	63.64	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 S...	[more]

BLAST of Moc08g34910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55770.1	6.9e-24	38.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	6.7e-19	41.22	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	2.9e-06	26.40	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G12880.1	8.5e-06	28.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	1.5e-05	29.79	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 29..174 e-value: 1.6E-30 score: 117.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..175 e-value: 2.8E-38 score: 129.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..174 e-value: 2.6E-30 score: 105.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..177 e-value: 2.3E-37 score: 130.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..176
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 15..177
None	No IPR available	PANTHER	PTHR31080:SF1	OS05G0360900 PROTEIN	coord: 15..177
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 33..174 e-value: 1.98436E-47 score: 149.789

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g34910.1	Moc08g34910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity