Moc08g34640 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g34640
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	chr8: 25303571 .. 25305245 (+)
RNA-Seq Expression	Moc08g34640
Synteny	Moc08g34640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTGGAAGGGGCGAAATCGTCGAAGGCGACGGGGCAGAGGATCGTGGTGGCGGTGGACGAGAGCGAGGAGAGTATGCTCGCCTTGCAATGGTGTCTCTCAAATCTCACTTCTCACGACACCAAAAACACTCTCGTTCTCCTCTATGTCAAGCCTTCTCCCGCCATCTCCATCTCCTCCTTCGATGCGCCTGGTCAGATTCAGATTCCGATTCCGATCACTAATTTTCCTCGCATCGGAGATATATTTGTGGTGATTGATTCTGATTGATTTTTTTTTTTTTTTGGCGATTTTTATGTATTGAAATGAAGGTTATGTGTTCTCGAACGATGTGATTTCGGCCATGGAAAAGCAGAGCAAGGATTTGGTTAGGTCGGTGATGAAGAGAGCTGAAGCGGTTTTTGTGAATTTCAACACTAACGTAATTAATCGAATCAACAATCTCTCATCTCGCTCTCTGTGATCTTCTGGCTTGTTTGTCTGTGATCTGTGATCTGTGATCTGTGTTTGTTATTTTATGAGCGGCATTTTTTCGAACACCTGATGCAGGTAAATTTAGAGAGAGTAGTGGGGACGGGAGATGCCAAGAACGTGATATGCCGCACAGTGGAGAAGATTGGAGCCGATACCTTGGTAATGGGATGCCATGGCTATGGCTTCTTCAAGAGGTGCGTATCTCCAAGTTTTGATTTATATACATTTTTTCTTGGGTTAGACTAGAAATTTAGAAGTTTTCAGTCTACTGATTTTTAAGTTTGTGTTAAATAGTATCTAATAGACAGACGGGCTTTAAATAGTTACCTAAACTTAAAAAATGTTTAATAAATTTCGTGCCCAATCTTCGCTCCAATCTGGATCTACTAATTCCCTAAACTTAAAAATGTTAATAGGCTCTAATCTTTCAATTTTGTGTAACCATTGTTCTTCAATATTAAAGATAAATGACAAAATAAAGTAGCCATAAAATGAAGTAAAACATGTAAACCTCCAGCCATCTTTTTTGTCATAAATTGAATGGTTTGCCATATTAGTAGAATAATGTGGGGCCCTTGAAATAAAAATAAAATATTTATAAATTGAATGAATAAATACTACTTTCTTAAAAGTCATTTAGCCTACTTTAAAAAAGTGACAATTTTGATCTACTCATTTTCATTTTTATTTCATTTCGATCAAAATTTAACATGAAACTTCTAAATTTTTTGAATGTAAATTTGTATAATAAATAAGTTGTAAGTATAAGGATTGAAATAAATAAAATCAAATTTACAATGACCAAAATAATAAAAATGGTAAGTACAGGAACTAGGATAAATAAAAGTTAGAATTACCATGGATAAATAATATTTTACCAATAATATCAATGATGTGTCAAATCGTGAATGGAATAGATAGATATTATTTTGCTTTACATTGTCTCATATGAATCAATATTTATACTATCAGCTTTTACATGGAACTATGATTGGAAATTTAATTCAGATTCTAGGGGTGGAATTGAAGCTAAAGTAATGTCTAGTTCAAAATTAAACCTTAAATACAAGGGTCTTAAATTATGTTGTGTAACACATTTTGTTGGTTTATTGGGGGTTGCAGGGCGTTGCTTGGAAGTGTGAGCGATCACTGCGCCAAATATGCCAAGTGTCCTGTGGTTATTGTGAAGCATCCTTAA

mRNA sequence

ATGGAACTGGAAGGGGCGAAATCGTCGAAGGCGACGGGGCAGAGGATCGTGGTGGCGGTGGACGAGAGCGAGGAGAGTATGCTCGCCTTGCAATGGTGTCTCTCAAATCTCACTTCTCACGACACCAAAAACACTCTCGTTCTCCTCTATGTCAAGCCTTCTCCCGCCATCTCCATCTCCTCCTTCGATGCGCCTGGTTATGTGTTCTCGAACGATGTGATTTCGGCCATGGAAAAGCAGAGCAAGGATTTGGTTAGGTCGGTGATGAAGAGAGCTGAAGCGGTTTTTGTGAATTTCAACACTAACGTAAATTTAGAGAGAGTAGTGGGGACGGGAGATGCCAAGAACGTGATATGCCGCACAGTGGAGAAGATTGGAGCCGATACCTTGGTAATGGGATGCCATGGCTATGGCTTCTTCAAGAGGGCGTTGCTTGGAAGTGTGAGCGATCACTGCGCCAAATATGCCAAGTGTCCTGTGGTTATTGTGAAGCATCCTTAA

Coding sequence (CDS)

ATGGAACTGGAAGGGGCGAAATCGTCGAAGGCGACGGGGCAGAGGATCGTGGTGGCGGTGGACGAGAGCGAGGAGAGTATGCTCGCCTTGCAATGGTGTCTCTCAAATCTCACTTCTCACGACACCAAAAACACTCTCGTTCTCCTCTATGTCAAGCCTTCTCCCGCCATCTCCATCTCCTCCTTCGATGCGCCTGGTTATGTGTTCTCGAACGATGTGATTTCGGCCATGGAAAAGCAGAGCAAGGATTTGGTTAGGTCGGTGATGAAGAGAGCTGAAGCGGTTTTTGTGAATTTCAACACTAACGTAAATTTAGAGAGAGTAGTGGGGACGGGAGATGCCAAGAACGTGATATGCCGCACAGTGGAGAAGATTGGAGCCGATACCTTGGTAATGGGATGCCATGGCTATGGCTTCTTCAAGAGGGCGTTGCTTGGAAGTGTGAGCGATCACTGCGCCAAATATGCCAAGTGTCCTGTGGTTATTGTGAAGCATCCTTAA

Protein sequence

MELEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSFDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP

Homology

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BLAST of Moc08g34640 vs. NCBI nr
Match: XP_022155260.1 (universal stress protein A-like protein [Momordica charantia])

HSP 1 Score: 326.6 bits (836), Expect = 1.2e-85
Identity = 165/166 (99.40%), Postives = 165/166 (99.40%), Query Frame = 0


Query: 1   MELEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISIS 60
           MELEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISIS
Sbjct: 1   MELEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISIS 60

Query: 61  SFDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICR 120
            FDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICR
Sbjct: 61  XFDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICR 120

Query: 121 TVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           TVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP
Sbjct: 121 TVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 166

[Close]

BLAST of Moc08g34640 vs. NCBI nr
Match: KAG7018016.1 (Universal stress protein A-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287.7 bits (735), Expect = 6.3e-74
Identity = 144/164 (87.80%), Postives = 152/164 (92.68%), Query Frame = 0


Query: 3   LEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSF 62
           +E AKS KA GQRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKPSPAISISSF
Sbjct: 1   MEAAKSLKANGQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPSPAISISSF 60

Query: 63  DAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTV 122
           DAPGYVFS+DVISAMEK  KDLV +VMKRAEAV+  FNTNVNLERVVG GDAKNVICRTV
Sbjct: 61  DAPGYVFSSDVISAMEKHGKDLVSAVMKRAEAVYAKFNTNVNLERVVGRGDAKNVICRTV 120

Query: 123 EKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           EK+GADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVK P
Sbjct: 121 EKLGADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVKQP 164

[Close]

BLAST of Moc08g34640 vs. NCBI nr
Match: XP_022935044.1 (universal stress protein PHOS32 [Cucurbita moschata])

HSP 1 Score: 286.2 bits (731), Expect = 1.8e-73
Identity = 143/164 (87.20%), Postives = 151/164 (92.07%), Query Frame = 0


Query: 3   LEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSF 62
           +E  KS KA GQRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKPSPAISISSF
Sbjct: 1   MEAVKSLKANGQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPSPAISISSF 60

Query: 63  DAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTV 122
           DAPGYVFS+DVISAMEK  KDLV +VMKRAEAV+  FNTNVNLERVVG GDAKNVICRTV
Sbjct: 61  DAPGYVFSSDVISAMEKHGKDLVSAVMKRAEAVYAKFNTNVNLERVVGRGDAKNVICRTV 120

Query: 123 EKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           EK+GADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVK P
Sbjct: 121 EKLGADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVKQP 164

[Close]

BLAST of Moc08g34640 vs. NCBI nr
Match: XP_022984075.1 (universal stress protein A-like protein [Cucurbita maxima])

HSP 1 Score: 285.0 bits (728), Expect = 4.1e-73
Identity = 143/162 (88.27%), Postives = 152/162 (93.83%), Query Frame = 0


Query: 3   LEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSF 62
           +E AKS KA GQRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKPSPAISISSF
Sbjct: 1   MEAAKSLKANGQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPSPAISISSF 60

Query: 63  DAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTV 122
           DAPGYVFS+DVISAMEKQ KDLV +VMKRAEAV+  FNTNV+LERVVG GDAKNVICRTV
Sbjct: 61  DAPGYVFSSDVISAMEKQGKDLVSAVMKRAEAVYAKFNTNVDLERVVGRGDAKNVICRTV 120

Query: 123 EKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVK 165
           EK+GADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVK
Sbjct: 121 EKLGADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVK 162

[Close]

BLAST of Moc08g34640 vs. NCBI nr
Match: XP_023529125.1 (universal stress protein A-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 142/164 (86.59%), Postives = 150/164 (91.46%), Query Frame = 0


Query: 3   LEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSF 62
           +E AKS K  GQRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKPSPAISISSF
Sbjct: 1   MEAAKSLKVNGQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPSPAISISSF 60

Query: 63  DAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTV 122
           DAPGYVFS+DVISAMEK  KDLV +VMKRAEAV+  FNTNVNLERVVG GDAKNVICRTV
Sbjct: 61  DAPGYVFSSDVISAMEKHGKDLVSAVMKRAEAVYAKFNTNVNLERVVGRGDAKNVICRTV 120

Query: 123 EKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           EK+ ADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVK P
Sbjct: 121 EKLEADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVKQP 164

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BLAST of Moc08g34640 vs. ExPASy Swiss-Prot
Match: Q8LGG8 (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 8.6e-10
Identity = 43/141 (30.50%), Postives = 72/141 (51.06%), Query Frame = 0


Query: 29  ALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSFDAPGYVFSNDVISAMEKQSKDLVRSV 88
           A +W L  +   +T +  +LL       + +   D  G+    D I A  +  +D+ +S 
Sbjct: 30  AFEWTLEKIVRSNTSDFKILL-------LHVQVVDEDGF-DDVDSIYASPEDFRDMRQSN 89

Query: 89  MKRAEAV---FVN--FNTNVNLERVVGTGDAKNVICRTVEKIGADTLVMGCHGYGFFKRA 148
             +   +   FVN      V  E  + TGD K+VIC+ V+++  D LV+G  G G F++ 
Sbjct: 90  KAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEVKRVRPDFLVVGSRGLGRFQKV 149

Query: 149 LLGSVSDHCAKYAKCPVVIVK 165
            +G+VS  C K+A+CPV+ +K
Sbjct: 150 FVGTVSAFCVKHAECPVMTIK 162

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BLAST of Moc08g34640 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.8e-08
Identity = 45/169 (26.63%), Postives = 80/169 (47.34%), Query Frame = 0


Query: 14  QRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAI------------SISS 73
           ++I VAVD SEES  A++W + +       + +VLL+V P+  +             I  
Sbjct: 48  RKIGVAVDLSEESSFAVRWAVDHYIR--PGDAVVLLHVSPTSVLFGADWGPLPLKTQIED 107

Query: 74  FDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRT 133
            +A       D  +    +  DL + + +      + F   ++   +V   D +  +C  
Sbjct: 108 PNAQPQPSQEDFDAFTSTKVADLAKPLKE------LGFPYKIH---IVKDHDMRERLCLE 167

Query: 134 VEKIGADTLVMGCHGYGFFKR----ALLGSVSDHCAKYAKCPVVIVKHP 167
           +E++G   ++MG  G+G  K+      LGSVSD+C  +  CPVV+V++P
Sbjct: 168 IERLGLSAVIMGSRGFGAEKKRGSDGKLGSVSDYCVHHCVCPVVVVRYP 205

[Close]

BLAST of Moc08g34640 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-06
Identity = 44/173 (25.43%), Postives = 77/173 (44.51%), Query Frame = 0


Query: 14  QRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPS-----------------PA 73
           ++I VAVD SEES  A++W + +       + +V+L+V P+                 P 
Sbjct: 45  RKIGVAVDLSEESAFAVRWAVDHYIR--PGDAVVILHVSPTSVLFGADWGPLPLQTPPPP 104

Query: 74  ISISSFDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKN 133
            + +   A       D  +    +  DL + + +        F   ++   +V   D + 
Sbjct: 105 SAATDPGAQPKPSQEDFDAFTSSKVADLAKPLKE------AGFPHKIH---IVKDHDMRE 164

Query: 134 VICRTVEKIGADTLVMGCHGYGFFKR---ALLGSVSDHCAKYAKCPVVIVKHP 167
            +C   E++    ++MG  G+G  KR     LGSVSD+C  +  CPVV+V++P
Sbjct: 165 RLCLETERLNLSAVIMGSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVRYP 206

[Close]

BLAST of Moc08g34640 vs. ExPASy Swiss-Prot
Match: Q57997 (Universal stress protein MJ0577 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0577 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-04
Identity = 26/96 (27.08%), Postives = 50/96 (52.08%), Query Frame = 0


Query: 69  FSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTVEKIGAD 128
           F N++ + + +++K+ + ++ K  E V         ++ ++  G     I +  E  G D
Sbjct: 69  FENELKNKLTEEAKNKMENIKKELEDV------GFKVKDIIVVGIPHEEIVKIAEDEGVD 128

Query: 129 TLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVK 165
            ++MG HG    K  LLGSV+++  K +  PV++VK
Sbjct: 129 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158

[Close]

BLAST of Moc08g34640 vs. ExPASy Swiss-Prot
Match: Q5HNJ5 (Putative universal stress protein SERP1273 OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=SERP1273 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.4e-04
Identity = 36/152 (23.68%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 14  QRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSFDAPGYVFSNDV 73
           + I++AVD S E+    +W  +       +N   L  V    + + SS++     F+   
Sbjct: 5   KNILIAVDGSHEA----EWAFNKAVGVAKRNDAQLTIVNVIDSRTYSSYEVYDAQFT--- 64

Query: 74  ISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTVEK-IGADTLVM 133
                ++SK     ++K  + V  N     N++  +  G  K +I + + + +GAD ++ 
Sbjct: 65  -----EKSKHFSEELLKGYKEVATNAGVK-NVDTRLEFGSPKAIIPKKLARDVGADLIMS 124

Query: 134 GCHGYGFFKRALLGSVSDHCAKYAKCPVVIVK 165
           G  G    +R ++GSVS+   ++A C V++V+
Sbjct: 125 GTSGLNAVERFIVGSVSEAIVRHAPCDVLVVR 143

[Close]

BLAST of Moc08g34640 vs. ExPASy TrEMBL
Match: A0A6J1DLY2 (universal stress protein A-like protein OS=Momordica charantia OX=3673 GN=LOC111022401 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 5.9e-86
Identity = 165/166 (99.40%), Postives = 165/166 (99.40%), Query Frame = 0


Query: 1   MELEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISIS 60
           MELEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISIS
Sbjct: 1   MELEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISIS 60

Query: 61  SFDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICR 120
            FDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICR
Sbjct: 61  XFDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICR 120

Query: 121 TVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           TVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP
Sbjct: 121 TVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 166

[Close]

BLAST of Moc08g34640 vs. ExPASy TrEMBL
Match: A0A6J1F4A3 (universal stress protein PHOS32 OS=Cucurbita moschata OX=3662 GN=LOC111442029 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 8.8e-74
Identity = 143/164 (87.20%), Postives = 151/164 (92.07%), Query Frame = 0


Query: 3   LEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSF 62
           +E  KS KA GQRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKPSPAISISSF
Sbjct: 1   MEAVKSLKANGQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPSPAISISSF 60

Query: 63  DAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTV 122
           DAPGYVFS+DVISAMEK  KDLV +VMKRAEAV+  FNTNVNLERVVG GDAKNVICRTV
Sbjct: 61  DAPGYVFSSDVISAMEKHGKDLVSAVMKRAEAVYAKFNTNVNLERVVGRGDAKNVICRTV 120

Query: 123 EKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           EK+GADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVK P
Sbjct: 121 EKLGADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVKQP 164

[Close]

BLAST of Moc08g34640 vs. ExPASy TrEMBL
Match: A0A6J1J7P2 (universal stress protein A-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482495 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.0e-73
Identity = 143/162 (88.27%), Postives = 152/162 (93.83%), Query Frame = 0


Query: 3   LEGAKSSKATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSF 62
           +E AKS KA GQRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKPSPAISISSF
Sbjct: 1   MEAAKSLKANGQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPSPAISISSF 60

Query: 63  DAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTV 122
           DAPGYVFS+DVISAMEKQ KDLV +VMKRAEAV+  FNTNV+LERVVG GDAKNVICRTV
Sbjct: 61  DAPGYVFSSDVISAMEKQGKDLVSAVMKRAEAVYAKFNTNVDLERVVGRGDAKNVICRTV 120

Query: 123 EKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVK 165
           EK+GADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVK
Sbjct: 121 EKLGADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVK 162

[Close]

BLAST of Moc08g34640 vs. ExPASy TrEMBL
Match: A0A1S3BY98 (universal stress protein A-like protein OS=Cucumis melo OX=3656 GN=LOC103494736 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.4e-71
Identity = 141/167 (84.43%), Postives = 153/167 (91.62%), Query Frame = 0


Query: 3   LEGAKSS---KATGQRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISI 62
           +E AK+S   K   QRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKP PAISI
Sbjct: 1   MEAAKNSLKAKGESQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPPPAISI 60

Query: 63  SSFDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVIC 122
           SSFDAPGYVFS++VISAMEKQSKDLV +VMKRAEAV+  F++NVNLERVVG GDAKNVIC
Sbjct: 61  SSFDAPGYVFSSEVISAMEKQSKDLVSAVMKRAEAVYAKFSSNVNLERVVGKGDAKNVIC 120

Query: 123 RTVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           RTVEK+GADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVKHP
Sbjct: 121 RTVEKLGADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVKHP 167

[Close]

BLAST of Moc08g34640 vs. ExPASy TrEMBL
Match: A0A0A0KTF3 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003620 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.1e-71
Identity = 141/165 (85.45%), Postives = 151/165 (91.52%), Query Frame = 0


Query: 4   EGAKSSKATG--QRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISS 63
           E  KS KA G  QRIVVAVDESEESM ALQWCLSNLTS DTKNTL+LLYVKP PAISISS
Sbjct: 3   EAKKSLKAKGESQRIVVAVDESEESMFALQWCLSNLTSPDTKNTLILLYVKPPPAISISS 62

Query: 64  FDAPGYVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRT 123
           FDAPGYVFS++VISAMEKQSKDLV +VMKRAEAV+  F++NVNLERVVG GDAKNVICR 
Sbjct: 63  FDAPGYVFSSEVISAMEKQSKDLVNAVMKRAEAVYAKFSSNVNLERVVGKGDAKNVICRI 122

Query: 124 VEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           VEK+GADTLVMGCHGYGFF+RALLGSVSD+CAKYAKCPVVIVKHP
Sbjct: 123 VEKLGADTLVMGCHGYGFFQRALLGSVSDYCAKYAKCPVVIVKHP 167

[Close]

BLAST of Moc08g34640 vs. TAIR 10
Match: AT3G62550.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 2.7e-43
Identity = 87/151 (57.62%), Postives = 112/151 (74.17%), Query Frame = 0


Query: 14  QRIVVAVDESEESMLALQWCLSNLTSHDTKNTLVLLYVKPSPAISISSFDAPGYVFSNDV 73
           ++IVVAVDESEESM AL W L NL  + + NTL+LLYVKP P    SS DA G++ + D 
Sbjct: 7   RKIVVAVDESEESMEALSWSLDNLFPYGSNNTLILLYVKP-PLPVYSSLDAAGFIVTGDP 66

Query: 74  ISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTVEKIGADTLVMG 133
           ++A++K   +LV SVM R+  V+ ++ +++N+ER VG GDAK VIC  V+K+  D LVMG
Sbjct: 67  VAALKKYEYELVESVMARSRTVYQDYESDINIERRVGRGDAKEVICNAVQKLRVDMLVMG 126

Query: 134 CHGYGFFKRALLGSVSDHCAKYAKCPVVIVK 165
            H YGFFKRALLGSVS++CAK  KCPVVIVK
Sbjct: 127 THDYGFFKRALLGSVSEYCAKRVKCPVVIVK 156

[Close]

BLAST of Moc08g34640 vs. TAIR 10
Match: AT1G09740.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 9.1e-23
Identity = 61/157 (38.85%), Postives = 91/157 (57.96%), Query Frame = 0


Query: 16  IVVAVDESEESMLALQWCLSN--LTSHDTKNTLVLLYVKPSPAISIS------SFDAPGY 75
           +VVAVD SE SM AL+W L N  L+S  + ++ V+L+V+PSP+++         F  P  
Sbjct: 10  VVVAVDGSEVSMEALRWALDNLKLSSSSSDSSFVVLHVQPSPSVAAGVSPGTIPFGGPSG 69

Query: 76  VFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTVEKIGA 135
           +      +A+E+  K +  ++++ A  +    + NV  + V+  GD K  IC  VE + A
Sbjct: 70  LEVPAFTAAIEQHQKRITDTILEHASQICAEKSVNVKTQVVI--GDPKYKICEAVENLHA 129

Query: 136 DTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVK 165
           D LVMG   YG  KR  LGSVS++C  +A CPVVI+K
Sbjct: 130 DLLVMGSRAYGRIKRMFLGSVSNYCTNHAHCPVVIIK 164

[Close]

BLAST of Moc08g34640 vs. TAIR 10
Match: AT2G47710.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 7.2e-20
Identity = 57/154 (37.01%), Postives = 90/154 (58.44%), Query Frame = 0


Query: 16  IVVAVDESEESMLALQWCLSNLTSHDTKN---TLVLLYVKPSPAISISSFDAPGYVFSND 75
           +VV VD+SE+S  AL+W L    +    N    L +++ KP+ A+S      PG   + +
Sbjct: 10  MVVGVDDSEQSTYALEWTLDRFFAPYAPNYPFKLFIVHAKPN-AVSAVGLAGPG---TAE 69

Query: 76  VISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGDAKNVICRTVEKIGADTLVM 135
           V+  ++   K     V+++A+A+  + + +  +  V   GDA+N++C  V+K  A  LV+
Sbjct: 70  VVPYVDADLKHTAAKVVEKAKAICQSRSVHGAVIEVF-EGDARNILCEVVDKHHASILVV 129

Query: 136 GCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           G HGYG  KRA+LGS SD+CA +A C V+IVK P
Sbjct: 130 GSHGYGAIKRAVLGSTSDYCAHHAHCSVMIVKKP 158

[Close]

BLAST of Moc08g34640 vs. TAIR 10
Match: AT3G11930.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 9.5e-20
Identity = 64/173 (36.99%), Postives = 100/173 (57.80%), Query Frame = 0


Query: 6   AKSSKATGQRIVVAVDESEESMLALQWCL---SNL-----TSHDTKNTLVLLYVKPSPAI 65
           A++   T +R+VVA+DES+ S  ALQW +   SNL      +      L +++V+ SP  
Sbjct: 25  AEAPSLTTKRMVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQ-SPFN 84

Query: 66  SISSFDA-PG---YVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTGD 125
             ++F A PG      S+ +I +++K  ++   +++ R  A+ +     +  E +V  G+
Sbjct: 85  HFAAFPAGPGGATVYASSSMIESVKKAQQETSAALLSR--ALQMCRAKQIRTETLVLEGE 144

Query: 126 AKNVICRTVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           AK +IC  VEK+  D LV+G  G G  KRA LGSVSD+CA +A CP++IVK P
Sbjct: 145 AKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPP 194

[Close]

BLAST of Moc08g34640 vs. TAIR 10
Match: AT3G11930.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 64/174 (36.78%), Postives = 100/174 (57.47%), Query Frame = 0


Query: 6   AKSSKATGQRIVVAVDESEESMLALQWCL---SNL-----TSHDTKNTLVLLYVKPSPAI 65
           A++   T +R+VVA+DES+ S  ALQW +   SNL      +      L +++V+ SP  
Sbjct: 25  AEAPSLTTKRMVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQ-SPFN 84

Query: 66  SISSFDA-PG----YVFSNDVISAMEKQSKDLVRSVMKRAEAVFVNFNTNVNLERVVGTG 125
             ++F A PG       S+ +I +++K  ++   +++ R  A+ +     +  E +V  G
Sbjct: 85  HFAAFPAGPGGATAVYASSSMIESVKKAQQETSAALLSR--ALQMCRAKQIRTETLVLEG 144

Query: 126 DAKNVICRTVEKIGADTLVMGCHGYGFFKRALLGSVSDHCAKYAKCPVVIVKHP 167
           +AK +IC  VEK+  D LV+G  G G  KRA LGSVSD+CA +A CP++IVK P
Sbjct: 145 EAKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPP 195

The following BLAST results are available for this feature:

BLAST of Moc08g34640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155260.1	1.2e-85	99.40	universal stress protein A-like protein [Momordica charantia]	[more]
KAG7018016.1	6.3e-74	87.80	Universal stress protein A-like protein [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_022935044.1	1.8e-73	87.20	universal stress protein PHOS32 [Cucurbita moschata]	[more]
XP_022984075.1	4.1e-73	88.27	universal stress protein A-like protein [Cucurbita maxima]	[more]
XP_023529125.1	1.5e-72	86.59	universal stress protein A-like protein [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc08g34640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LGG8	8.6e-10	30.50	Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g0...	[more]
Q8VYN9	2.8e-08	26.63	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]
Q8L4N1	1.5e-06	25.43	Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S...	[more]
Q57997	1.9e-04	27.08	Universal stress protein MJ0577 OS=Methanocaldococcus jannaschii (strain ATCC 43...	[more]
Q5HNJ5	5.4e-04	23.68	Putative universal stress protein SERP1273 OS=Staphylococcus epidermidis (strain...	[more]

BLAST of Moc08g34640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLY2	5.9e-86	99.40	universal stress protein A-like protein OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1F4A3	8.8e-74	87.20	universal stress protein PHOS32 OS=Cucurbita moschata OX=3662 GN=LOC111442029 PE...	[more]
A0A6J1J7P2	2.0e-73	88.27	universal stress protein A-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482...	[more]
A0A1S3BY98	1.4e-71	84.43	universal stress protein A-like protein OS=Cucumis melo OX=3656 GN=LOC103494736 ...	[more]
A0A0A0KTF3	4.1e-71	85.45	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003620 PE=4 SV...	[more]

BLAST of Moc08g34640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62550.1	2.7e-43	57.62	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT1G09740.1	9.1e-23	38.85	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT2G47710.1	7.2e-20	37.01	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G11930.1	9.5e-20	36.99	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G11930.2	1.2e-19	36.78	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006015	Universal stress protein A family	PRINTS	PR01438	UNVRSLSTRESS	coord: 142..164 score: 40.52 coord: 124..136 score: 39.69 coord: 13..31 score: 31.79
IPR006016	UspA	PFAM	PF00582	Usp	coord: 14..164 e-value: 4.3E-28 score: 98.6
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 8..166 e-value: 9.0E-38 score: 132.2
None	No IPR available	PANTHER	PTHR31964	ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN	coord: 5..165
None	No IPR available	PANTHER	PTHR31964:SF126	ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN	coord: 5..165
None	No IPR available	CDD	cd00293	USP_Like	coord: 15..163 e-value: 7.05445E-30 score: 103.989
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 14..165

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g34640.1	Moc08g34640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005794	Golgi apparatus
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding