Moc08g34140 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g34140
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr8: 24907160 .. 24907897 (-)
RNA-Seq ExpressionMoc08g34140
SyntenyMoc08g34140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCAGCAGAGAAGTCAGGTTTTGAAGGAATCATGAAGTTCGATGGGGCCAATTTTGGATATTGGAAGATGCAGATTAAGGATTACTTAACCTGCAAGAAGTTGAATAAAGATATAAAAGAACGACCAGCAGGGATGAAAGATGAAGATTGGGTAGAGACGAATGAATAGGTAGTTGCCTTTATCAGATTGTGTTTGTCGATGAATGTGGCAAGTCTCGTATCAAACGAAACGACAACAATAAATCTGATAAAATCACTGGAGAATAGGTACGAGAAACCCTTAGCTAATAATAAGGTATATCTCTTAAGAATATTCTTTAACATTCAAATGGAAGAGAATACTTCTGTCAATTCCCACATAAATGAGGTCACAAAATTATTCAATTAATTGGCATCAGTTAAGATTATTTTTAGTGACGAAGTGAATGCTATTCAGTTGCTAACATCTTTACCTGAAAGTTGGGAAACGATGAAGACAGTAGTGTCCAATTCGTTAGGAGACAAATCTTTGAAATTTTTAGAAATTTGTGATGCAGCAATTGCTGAAGAAATTCGCAGAAAGGGGAATGGAAAAGAATCTACTTCCAAACAAGACTCAGCATTGACTGTCGAAAAAGGTAAAGAGAAGGTTGCGTCTGATGACAAGCAGAAGCATAGTAGGAGAGATTGGAAGAGAGATGTGGAATGTTTTCACTGCCACAAAAAAGACCACATCAAGACTGTAGAATATTAA

mRNA sequence

ATGAGATCAGCAGAGAAGTCAGGTTTTGAAGGAATCATGAAGTTCGATGGGGCCAATTTTGGATATTGGAAGATGCAGATTAAGGATTACTTAACCTGCAAGAAGTTGAATAAAGATATAAAAGAACGACCAGCAGGGATGAAAGATGAAGATTGGTTGCTAACATCTTTACCTGAAAGTTGGGAAACGATGAAGACAGTAGTGTCCAATTCGTTAGGAGACAAATCTTTGAAATTTTTAGAAATTTGTGATGCAGCAATTGCTGAAGAAATTCGCAGAAAGGGGAATGGAAAAGAATCTACTTCCAAACAAGACTCAGCATTGACTGTCGAAAAAGGTAAAGAGAAGGTTGCGTCTGATGACAAGCAGAAGCATAGTAGGAGAGATTGGAAGAGAGATGTGGAATGTTTTCACTGCCACAAAAAAGACCACATCAAGACTGTAGAATATTAA

Coding sequence (CDS)

ATGAGATCAGCAGAGAAGTCAGGTTTTGAAGGAATCATGAAGTTCGATGGGGCCAATTTTGGATATTGGAAGATGCAGATTAAGGATTACTTAACCTGCAAGAAGTTGAATAAAGATATAAAAGAACGACCAGCAGGGATGAAAGATGAAGATTGGTTGCTAACATCTTTACCTGAAAGTTGGGAAACGATGAAGACAGTAGTGTCCAATTCGTTAGGAGACAAATCTTTGAAATTTTTAGAAATTTGTGATGCAGCAATTGCTGAAGAAATTCGCAGAAAGGGGAATGGAAAAGAATCTACTTCCAAACAAGACTCAGCATTGACTGTCGAAAAAGGTAAAGAGAAGGTTGCGTCTGATGACAAGCAGAAGCATAGTAGGAGAGATTGGAAGAGAGATGTGGAATGTTTTCACTGCCACAAAAAAGACCACATCAAGACTGTAGAATATTAA

Protein sequence

MRSAEKSGFEGIMKFDGANFGYWKMQIKDYLTCKKLNKDIKERPAGMKDEDWLLTSLPESWETMKTVVSNSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVEKGKEKVASDDKQKHSRRDWKRDVECFHCHKKDHIKTVEY
Homology
BLAST of Moc08g34140 vs. NCBI nr
Match: XP_022138046.1 (kirola-like [Momordica charantia])

HSP 1 Score: 92.0 bits (227), Expect = 4.6e-15
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 0

Query: 64  MKTVVSNSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVEKGKEKVASDDKQ 123
           MKT VSNSLGDKSLKF EICDAAIAEEIRRK +GKE TS Q SAL VEKGKEKVASDD++
Sbjct: 1   MKTAVSNSLGDKSLKFSEICDAAIAEEIRRKRSGKEYTSLQGSALAVEKGKEKVASDDRR 60

BLAST of Moc08g34140 vs. NCBI nr
Match: RVW93053.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 87.8 bits (216), Expect = 8.6e-14
Identity = 54/129 (41.86%), Postives = 75/129 (58.14%), Query Frame = 0

Query: 11  GIMKFDGANFGYWKMQIKDYLTCKKLNKD-IKERPAGMKDEDWLLTSLPESWETMKTVVS 70
           GI KFDG +F YW+MQI+DYL  +KL+   +  +P  MK E+W    L +SWE M+  VS
Sbjct: 10  GIEKFDGTHFAYWRMQIEDYLYGRKLHLPLLGPKPENMKAEEWRF--LTDSWEAMRMAVS 69

Query: 71  NSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVEKGKEKVASDDKQKHSRRD 130
           NS G + LK+ +I D  +AEEIRR+  G+  TS   SAL +E G   + S  +   +  D
Sbjct: 70  NSTGKEKLKYNDIRDLILAEEIRRRDAGE--TSIYGSALNLETGTLYMTSSPRDTIAVAD 129

Query: 131 WKRDVECFH 139
              D   +H
Sbjct: 130 ASTDTSLWH 134

BLAST of Moc08g34140 vs. NCBI nr
Match: KAB5573948.1 (hypothetical protein DKX38_001142 [Salix brachista])

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-12
Identity = 51/121 (42.15%), Postives = 68/121 (56.20%), Query Frame = 0

Query: 11  GIMKFDGANFGYWKMQIKDYLTCKKLNKD-IKERPAGMKDEDWL-------------LTS 70
           GI KFDG +F YWKMQI+DYL  KKL+   + ++P  MKDE+W+             LT 
Sbjct: 10  GIEKFDGRDFSYWKMQIEDYLYGKKLHLPLLGQKPESMKDEEWISLDKQVLGLIRLTLTR 69

Query: 71  ------LPESWETMKTVVSNSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTV 112
                 + ESWE M+T +SNS     L + +I D  + EE+ R+  G+ STS   SAL V
Sbjct: 70  KVAHNVIKESWEAMRTTISNSARKSKLNYDDIRDLILGEEVCRRDAGESSTS--GSALNV 128

BLAST of Moc08g34140 vs. NCBI nr
Match: XP_023894133.1 (receptor-like protein EIX2 [Quercus suber])

HSP 1 Score: 82.4 bits (202), Expect = 3.6e-12
Identity = 49/113 (43.36%), Postives = 69/113 (61.06%), Query Frame = 0

Query: 11  GIMKFDGANFGYWKMQIKDYLTCKKLNKDI-KERPAGMKDEDWLLTSLPESWETMKTVVS 70
           GI KFDG NFGYWKMQIKDYL  KKL+  + +++P  +K E+W L  L  SW+ M+  V+
Sbjct: 10  GIEKFDGTNFGYWKMQIKDYLYGKKLHLPLFRKKPKTIKHEEWNL--LINSWKAMRMAVN 69

Query: 71  NSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVE---KGKEKVAS 120
           NS G   LK+ +I D  ++EEI +K    +  + QD A   E   +G+ +  S
Sbjct: 70  NSAGKSKLKYDDIRDLILSEEICKKDVNID--NPQDQAFVTENKSRGRSRAGS 118

BLAST of Moc08g34140 vs. NCBI nr
Match: CAN65867.1 (hypothetical protein VITISV_034935 [Vitis vinifera])

HSP 1 Score: 80.5 bits (197), Expect = 1.4e-11
Identity = 64/204 (31.37%), Postives = 95/204 (46.57%), Query Frame = 0

Query: 4   AEKSG-FEGIMKFDGANFGYWKMQIKDYLTCKKLNKD-IKERPAGMKDEDW--------- 63
           AE++G   GI KFDG +F YW+MQI+DYL  +KL+   +  +P  MK E+W         
Sbjct: 2   AEEAGKASGIEKFDGTDFAYWRMQIEDYLYGRKLHLPLLGTKPESMKAEEWALLDRQVLG 61

Query: 64  ----------------------------------------LLTSLPESWETMKTVVSNSL 123
                                                   +L SLP SWE M+  VSNS 
Sbjct: 62  VIRLTLSRSVAHNVVKEKTTADLMKALSEIDFDDEIRALIVLASLPNSWEAMRMAVSNST 121

Query: 124 GDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVE-KGKEKVASDDKQKHSRRDWK 147
           G + LK+ +I D  +AEEIRR+  G+  TS   SAL +E +G+    + ++ + + R+  
Sbjct: 122 GKEKLKYNDIRDLILAEEIRRRDAGE--TSGSGSALNLETRGRGNNRNSNQGRSNSRNSN 181

BLAST of Moc08g34140 vs. ExPASy TrEMBL
Match: A0A6J1C8L4 (kirola-like OS=Momordica charantia OX=3673 GN=LOC111009302 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.2e-15
Identity = 50/60 (83.33%), Postives = 53/60 (88.33%), Query Frame = 0

Query: 64  MKTVVSNSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVEKGKEKVASDDKQ 123
           MKT VSNSLGDKSLKF EICDAAIAEEIRRK +GKE TS Q SAL VEKGKEKVASDD++
Sbjct: 1   MKTAVSNSLGDKSLKFSEICDAAIAEEIRRKRSGKEYTSLQGSALAVEKGKEKVASDDRR 60

BLAST of Moc08g34140 vs. ExPASy TrEMBL
Match: A0A438I8L6 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_2699 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 4.2e-14
Identity = 54/129 (41.86%), Postives = 75/129 (58.14%), Query Frame = 0

Query: 11  GIMKFDGANFGYWKMQIKDYLTCKKLNKD-IKERPAGMKDEDWLLTSLPESWETMKTVVS 70
           GI KFDG +F YW+MQI+DYL  +KL+   +  +P  MK E+W    L +SWE M+  VS
Sbjct: 10  GIEKFDGTHFAYWRMQIEDYLYGRKLHLPLLGPKPENMKAEEWRF--LTDSWEAMRMAVS 69

Query: 71  NSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVEKGKEKVASDDKQKHSRRD 130
           NS G + LK+ +I D  +AEEIRR+  G+  TS   SAL +E G   + S  +   +  D
Sbjct: 70  NSTGKEKLKYNDIRDLILAEEIRRRDAGE--TSIYGSALNLETGTLYMTSSPRDTIAVAD 129

Query: 131 WKRDVECFH 139
              D   +H
Sbjct: 130 ASTDTSLWH 134

BLAST of Moc08g34140 vs. ExPASy TrEMBL
Match: A0A803LFR5 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.6e-13
Identity = 49/114 (42.98%), Postives = 64/114 (56.14%), Query Frame = 0

Query: 14  KFDGANFGYWKMQIKDYLTCKKLNKDIKE-RPAGMKDEDWLL------------------ 73
           KFDGA+FG+WKMQIKDYL  KKL + + E +P GMKDEDW L                  
Sbjct: 318 KFDGADFGFWKMQIKDYLYQKKLYEPLLEKKPEGMKDEDWNLLDRQALGVIRLTFSRNIA 377

Query: 74  ------TSLPESWETMKTVVSNSLGDKSLKFLEICDAAIAEEIRRKGNGKESTS 103
                  +  +SW    T VS+S G   LKF ++ D  ++EEIRR+ +G+ STS
Sbjct: 378 FNIAKEKTTTDSWNATVTTVSSSSGSNKLKFNDVRDLVLSEEIRRRESGELSTS 431

BLAST of Moc08g34140 vs. ExPASy TrEMBL
Match: A0A0D2ZVN0 (Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.1e-13
Identity = 49/151 (32.45%), Postives = 79/151 (52.32%), Query Frame = 0

Query: 5   EKSGFEGIMKFDGANFGYWKMQIKDYLTCKKLNKDIKERPAGMKDEDWLLTSLPE----S 64
           E      I KFDG ++ +W MQI+DYL  KKL++ + ++P  M  ++W L         S
Sbjct: 4   EDGSAHSIRKFDGTDYAFWSMQIEDYLYEKKLHQPLSKKPEKMDQDEWELLDRQVMGVIS 63

Query: 65  WETMKTVVSNSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTVEKGKEKVASD 124
           WE M+  VSN +  + LKF ++ D  + EE+RR  +G+ STS   +     +  ++    
Sbjct: 64  WEPMRVAVSNYVSTQKLKFNDVRDRILGEEVRRIDSGEASTSSAFNVENRGRNPDRNNRS 123

Query: 125 DKQKHSRRDW-----KRDVECFHCHKKDHIK 147
           + +  SR  W     ++  EC++C K  HIK
Sbjct: 124 NGRSKSRNGWGQSKFRQPTECWNCGKTGHIK 154

BLAST of Moc08g34140 vs. ExPASy TrEMBL
Match: A0A5N5P413 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_001142 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.0e-12
Identity = 51/121 (42.15%), Postives = 68/121 (56.20%), Query Frame = 0

Query: 11  GIMKFDGANFGYWKMQIKDYLTCKKLNKD-IKERPAGMKDEDWL-------------LTS 70
           GI KFDG +F YWKMQI+DYL  KKL+   + ++P  MKDE+W+             LT 
Sbjct: 10  GIEKFDGRDFSYWKMQIEDYLYGKKLHLPLLGQKPESMKDEEWISLDKQVLGLIRLTLTR 69

Query: 71  ------LPESWETMKTVVSNSLGDKSLKFLEICDAAIAEEIRRKGNGKESTSKQDSALTV 112
                 + ESWE M+T +SNS     L + +I D  + EE+ R+  G+ STS   SAL V
Sbjct: 70  KVAHNVIKESWEAMRTTISNSARKSKLNYDDIRDLILGEEVCRRDAGESSTS--GSALNV 128

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138046.14.6e-1583.33kirola-like [Momordica charantia][more]
RVW93053.18.6e-1441.86Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
KAB5573948.12.1e-1242.15hypothetical protein DKX38_001142 [Salix brachista][more]
XP_023894133.13.6e-1243.36receptor-like protein EIX2 [Quercus suber][more]
CAN65867.11.4e-1131.37hypothetical protein VITISV_034935 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1C8L42.2e-1583.33kirola-like OS=Momordica charantia OX=3673 GN=LOC111009302 PE=3 SV=1[more]
A0A438I8L64.2e-1441.86Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A803LFR51.6e-1342.98Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
A0A0D2ZVN02.1e-1332.45Uncharacterized protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1[more]
A0A5N5P4131.0e-1242.15Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_001142 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..130
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..130
NoneNo IPR availablePANTHERPTHR34676:SF1ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATEDcoord: 52..147
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 11..52
NoneNo IPR availablePANTHERPTHR34676:SF1ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATEDcoord: 11..52
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 52..147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g34140.1Moc08g34140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0005488 binding