Moc08g33510 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g33510
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionnitrate regulatory gene2 protein-like
Locationchr8: 24338457 .. 24340368 (+)
RNA-Seq ExpressionMoc08g33510
SyntenyMoc08g33510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTGCGCCACCTCCAAGCTCCACAATCTCCCGGCGGTGGCTCTGTGCCGCGACCGCTGCAAATTTCTTGACCAAGCTCTGGTTTTCAGCCATGCCCTCGCCGATTCCCACTCCGCCTACGCAGAATCCCTCACTATTATGGCCGCAGCTCTCCACCGTCTGTTTGACCAGGACGCCGCCGGCGATCCGAAGGATTCTCCGCCGGCTGTCGCCCCTTCGCCTGACAGTTCTCCCTCTGATTCCGATTCCGATGAAGAAGAAGAAGATGGGTCTTTTACTCCTGAGAAAACACGGTCTCCCCCTTCCCCAGTTGGAAATTTCATGTTCTCGAATTACGACTCGGCCAGGGGACAGCAGCCGCCGCCGCCGCCGCCGACCGGCTCGTCTTGGGATTTCTTGAATTTCTTCGAAACCTACGAAAGATACGAGCAGCCCGTTTTCAATTGGGACCGGGAAGGAGGAGCAGCAGATCAAAAACCCAGAACAATCGCGAAGAAGAAGAAGAAGAAGAAAGTAGCCGCGGTTGTGGATTCTAAGAACACGAAGGAATCAAATCAACTGGAAAAGAAGAGCGATTCGAAAATGGGTGTCTCCGATGCGATGAGAGAACTCAAGGGCTCCTTCGAAAAGGCGTCTGAATCGGGAAATTCCATTTCCAAGTTGATTTCTTATGGGCAAAGACCAGATTTCACTAAAGGTATCATTTTTCTTCTTCTTCTTCTTCTTCTTCTACTCTGTAAATTGCTCTGTTTTCATCCTCCGATTGATTTCTTGTTTTCCTTACAGATACAAGGATTGGCTCGCGGGGTTTATCTTCTACTCTGAAGAAGCTATGCGTGTGGGAGCAGAAACTGTACCTTGAAGTGAAGGTTATTCATATATACATCTTTTTCAATTTAAAAAACTGGGTTTTTCTGATTCTGGGGTTTGGGATTGGTTTAGGCGGAGGAGAAATTGCGTATGATTTTGGAGAAGAAGTGGCGGCAGGTGAAGCATTTGGTGGAGAAGAAGGCCGATGCTCGGAAAGTTGAATCTGTTCGAACATTGATCAGAAATTTATCCATCAAATTGAAAGTTTCGATTCAGGTTGTGGATAGGATTTCGATTTCCATAAGCAAGTTGAGAGATGAAGAGTTTTTGGCAGAGATTACCCAATTGATTCATGGGTATGAATCAATTCCTTTGACCCAATTTCAAACTTACATTTTTCTTTTTGTTGGATTAATCAAACATTTTCTTGCACAGATTTCTCAGCATGTGGAAATCCATGCTAGAATCCCACAAGCAACAAACCCAAGTGCTAACAGAATCCAGAACTTGGGAATTAGCCCTGCAGAACGGGAAACTCGACGACATTCATTTGGAAGCCGCCATGGATTTGAAGCTCCACCTCGAGAATTGGCGAACGGCTTTCTCCACATTAATCACAACCCAAAACGACTGCATCAAATCCCTCAACGGCTGGCTCCTCCGCTGCCTTCTGCACGAACCCGAGCCCGAAGAGACCCCCAACGGCGCCGGCCCGCTGCCGTTCTCCCCGGCCAGAATCGGCGCCCCGCCGGCGTTCGTAATCTGCCACCTCTGGTCGGAAACCGTCGACAAATTCTCCGGCGAGGAAGTCTCGGAGGCGATTTGTGGGGTTGTTGCCGTCTTAGACGAGGCGCTGGCGGAGCAGAGTGAGGATTTGCAGAGATTGGGGCTGGCGAGTAAGATGAAGATGAAGAAGACAGCGGCGGCGGTGACGGCGGAGGCTGTGCATAAGAGGGGTGAGTTTAGTGGATGTGAGGTTCAATTGGGGATGAAGCGGATTTTTGCGGCGTTGGCGAGGTTTTGTTGGGACTCCATTGCAGCTTATGAGGAGCTCTGTTCGGCCATGGAGAAGCAGGAACAGAGCCAGGTCCATTGCTAG

mRNA sequence

ATGGGCTGCGCCACCTCCAAGCTCCACAATCTCCCGGCGGTGGCTCTGTGCCGCGACCGCTGCAAATTTCTTGACCAAGCTCTGGTTTTCAGCCATGCCCTCGCCGATTCCCACTCCGCCTACGCAGAATCCCTCACTATTATGGCCGCAGCTCTCCACCGTCTGTTTGACCAGGACGCCGCCGGCGATCCGAAGGATTCTCCGCCGGCTGTCGCCCCTTCGCCTGACAGTTCTCCCTCTGATTCCGATTCCGATGAAGAAGAAGAAGATGGGTCTTTTACTCCTGAGAAAACACGGTCTCCCCCTTCCCCAGTTGGAAATTTCATGTTCTCGAATTACGACTCGGCCAGGGGACAGCAGCCGCCGCCGCCGCCGCCGACCGGCTCGTCTTGGGATTTCTTGAATTTCTTCGAAACCTACGAAAGATACGAGCAGCCCGTTTTCAATTGGGACCGGGAAGGAGGAGCAGCAGATCAAAAACCCAGAACAATCGCGAAGAAGAAGAAGAAGAAGAAAGTAGCCGCGGTTGTGGATTCTAAGAACACGAAGGAATCAAATCAACTGGAAAAGAAGAGCGATTCGAAAATGGGTGTCTCCGATGCGATGAGAGAACTCAAGGGCTCCTTCGAAAAGGCGTCTGAATCGGGAAATTCCATTTCCAAGTTGATTTCTTATGGGCAAAGACCAGATTTCACTAAAGATACAAGGATTGGCTCGCGGGGTTTATCTTCTACTCTGAAGAAGCTATGCGTGTGGGAGCAGAAACTGTACCTTGAAGTGAAGGCGGAGGAGAAATTGCGTATGATTTTGGAGAAGAAGTGGCGGCAGGTGAAGCATTTGGTGGAGAAGAAGGCCGATGCTCGGAAAGTTGAATCTGTTCGAACATTGATCAGAAATTTATCCATCAAATTGAAAGTTTCGATTCAGGTTGTGGATAGGATTTCGATTTCCATAAGCAAGTTGAGAGATGAAGAGTTTTTGGCAGAGATTACCCAATTGATTCATGGATTTCTCAGCATGTGGAAATCCATGCTAGAATCCCACAAGCAACAAACCCAAGTGCTAACAGAATCCAGAACTTGGGAATTAGCCCTGCAGAACGGGAAACTCGACGACATTCATTTGGAAGCCGCCATGGATTTGAAGCTCCACCTCGAGAATTGGCGAACGGCTTTCTCCACATTAATCACAACCCAAAACGACTGCATCAAATCCCTCAACGGCTGGCTCCTCCGCTGCCTTCTGCACGAACCCGAGCCCGAAGAGACCCCCAACGGCGCCGGCCCGCTGCCGTTCTCCCCGGCCAGAATCGGCGCCCCGCCGGCGTTCGTAATCTGCCACCTCTGGTCGGAAACCGTCGACAAATTCTCCGGCGAGGAAGTCTCGGAGGCGATTTGTGGGGTTGTTGCCGTCTTAGACGAGGCGCTGGCGGAGCAGAGTGAGGATTTGCAGAGATTGGGGCTGGCGAGTAAGATGAAGATGAAGAAGACAGCGGCGGCGGTGACGGCGGAGGCTGTGCATAAGAGGGGTGAGTTTAGTGGATGTGAGGTTCAATTGGGGATGAAGCGGATTTTTGCGGCGTTGGCGAGGTTTTGTTGGGACTCCATTGCAGCTTATGAGGAGCTCTGTTCGGCCATGGAGAAGCAGGAACAGAGCCAGGTCCATTGCTAG

Coding sequence (CDS)

ATGGGCTGCGCCACCTCCAAGCTCCACAATCTCCCGGCGGTGGCTCTGTGCCGCGACCGCTGCAAATTTCTTGACCAAGCTCTGGTTTTCAGCCATGCCCTCGCCGATTCCCACTCCGCCTACGCAGAATCCCTCACTATTATGGCCGCAGCTCTCCACCGTCTGTTTGACCAGGACGCCGCCGGCGATCCGAAGGATTCTCCGCCGGCTGTCGCCCCTTCGCCTGACAGTTCTCCCTCTGATTCCGATTCCGATGAAGAAGAAGAAGATGGGTCTTTTACTCCTGAGAAAACACGGTCTCCCCCTTCCCCAGTTGGAAATTTCATGTTCTCGAATTACGACTCGGCCAGGGGACAGCAGCCGCCGCCGCCGCCGCCGACCGGCTCGTCTTGGGATTTCTTGAATTTCTTCGAAACCTACGAAAGATACGAGCAGCCCGTTTTCAATTGGGACCGGGAAGGAGGAGCAGCAGATCAAAAACCCAGAACAATCGCGAAGAAGAAGAAGAAGAAGAAAGTAGCCGCGGTTGTGGATTCTAAGAACACGAAGGAATCAAATCAACTGGAAAAGAAGAGCGATTCGAAAATGGGTGTCTCCGATGCGATGAGAGAACTCAAGGGCTCCTTCGAAAAGGCGTCTGAATCGGGAAATTCCATTTCCAAGTTGATTTCTTATGGGCAAAGACCAGATTTCACTAAAGATACAAGGATTGGCTCGCGGGGTTTATCTTCTACTCTGAAGAAGCTATGCGTGTGGGAGCAGAAACTGTACCTTGAAGTGAAGGCGGAGGAGAAATTGCGTATGATTTTGGAGAAGAAGTGGCGGCAGGTGAAGCATTTGGTGGAGAAGAAGGCCGATGCTCGGAAAGTTGAATCTGTTCGAACATTGATCAGAAATTTATCCATCAAATTGAAAGTTTCGATTCAGGTTGTGGATAGGATTTCGATTTCCATAAGCAAGTTGAGAGATGAAGAGTTTTTGGCAGAGATTACCCAATTGATTCATGGATTTCTCAGCATGTGGAAATCCATGCTAGAATCCCACAAGCAACAAACCCAAGTGCTAACAGAATCCAGAACTTGGGAATTAGCCCTGCAGAACGGGAAACTCGACGACATTCATTTGGAAGCCGCCATGGATTTGAAGCTCCACCTCGAGAATTGGCGAACGGCTTTCTCCACATTAATCACAACCCAAAACGACTGCATCAAATCCCTCAACGGCTGGCTCCTCCGCTGCCTTCTGCACGAACCCGAGCCCGAAGAGACCCCCAACGGCGCCGGCCCGCTGCCGTTCTCCCCGGCCAGAATCGGCGCCCCGCCGGCGTTCGTAATCTGCCACCTCTGGTCGGAAACCGTCGACAAATTCTCCGGCGAGGAAGTCTCGGAGGCGATTTGTGGGGTTGTTGCCGTCTTAGACGAGGCGCTGGCGGAGCAGAGTGAGGATTTGCAGAGATTGGGGCTGGCGAGTAAGATGAAGATGAAGAAGACAGCGGCGGCGGTGACGGCGGAGGCTGTGCATAAGAGGGGTGAGTTTAGTGGATGTGAGGTTCAATTGGGGATGAAGCGGATTTTTGCGGCGTTGGCGAGGTTTTGTTGGGACTCCATTGCAGCTTATGAGGAGCTCTGTTCGGCCATGGAGAAGCAGGAACAGAGCCAGGTCCATTGCTAG

Protein sequence

MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDAAGDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSARGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSKNTKESNQLEKKSDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAICGVVAVLDEALAEQSEDLQRLGLASKMKMKKTAAAVTAEAVHKRGEFSGCEVQLGMKRIFAALARFCWDSIAAYEELCSAMEKQEQSQVHC
Homology
BLAST of Moc08g33510 vs. NCBI nr
Match: XP_022153114.1 (uncharacterized protein LOC111020693, partial [Momordica charantia])

HSP 1 Score: 822.4 bits (2123), Expect = 2.4e-234
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60
           MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA
Sbjct: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60

Query: 61  AGDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSARGQQ 120
           AGDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSARGQQ
Sbjct: 61  AGDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSARGQQ 120

Query: 121 PPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSK 180
           PPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSK
Sbjct: 121 PPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSK 180

Query: 181 NTKESNQLEKKSDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDFTKDTRIGSR 240
           NTKESNQLEKKSDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDFTKDTRIGSR
Sbjct: 181 NTKESNQLEKKSDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDFTKDTRIGSR 240

Query: 241 GLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNL 300
           GLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNL
Sbjct: 241 GLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNL 300

Query: 301 SIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRT 360
           SIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRT
Sbjct: 301 SIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRT 360

Query: 361 WELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEP 420
           WELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEP
Sbjct: 361 WELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEP 420

Query: 421 EETPN 426
           EETPN
Sbjct: 421 EETPN 425

BLAST of Moc08g33510 vs. NCBI nr
Match: XP_008464870.1 (PREDICTED: uncharacterized protein LOC103502634 [Cucumis melo])

HSP 1 Score: 709.9 bits (1831), Expect = 1.7e-200
Identity = 402/578 (69.55%), Postives = 454/578 (78.55%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQALV +H+L DSHSAYA+SL   A+AL RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALVSTHSLIDSHSAYADSLDKTASALRRLFDQDG 60

Query: 61  --AAGDPKDS-PPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSA 120
               GD K + PP  AP  D S SDSDS+EEEEDG F  EK  SP  PVG+FMFSNYDSA
Sbjct: 61  ETGGGDLKCAPPPPAAPKKDRSDSDSDSEEEEEDGCFDQEKPLSP--PVGSFMFSNYDSA 120

Query: 121 RGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQK-PRTIAKKKKKKKVAA 180
           RG QPPPPPPTGSSWDF NFF++YERYEQP+FNWDREG  AD+K P T    KKKKK A 
Sbjct: 121 RG-QPPPPPPTGSSWDFFNFFDSYERYEQPIFNWDREG--ADRKLPTTKVVAKKKKKPAL 180

Query: 181 VVDSKN---TKESNQLEKK-SDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDF 240
           VVDSK    T++SNQ EKK  D KM V D MRE+KGSFEKASES NSISKL+SYGQR  F
Sbjct: 181 VVDSKKNEVTEKSNQPEKKIDDPKMSVLDLMREIKGSFEKASESSNSISKLLSYGQRMSF 240

Query: 241 TKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVE 300
            KDTR GSRGL STLKKL VWEQKLYLEVKAEE+LRM+LEKK RQ+K+L+EKKADARK++
Sbjct: 241 CKDTRTGSRGLLSTLKKLSVWEQKLYLEVKAEERLRMLLEKKCRQLKNLMEKKADARKID 300

Query: 301 SVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQ 360
            +R  IRNLSIKLK+SIQVVDRISI+ISKLRDEEF AE+ +LI G  SMWKSMLE+HKQQ
Sbjct: 301 PLRNSIRNLSIKLKISIQVVDRISITISKLRDEEFFAEMNELIRGLQSMWKSMLETHKQQ 360

Query: 361 TQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLL 420
           TQ LT+ + +E ++ NG LDD HLEAAMDLKL LENWRT F  LI TQ DCIK+LNGWLL
Sbjct: 361 TQALTDPKPFE-SILNGALDDTHLEAAMDLKLELENWRTNFIELIATQKDCIKALNGWLL 420

Query: 421 RCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAICGVVAV 480
           RCLL+EP+PEETPNG  P PFSP RIGAPP FVI HLWS+T D FS ++VSEAI G+V  
Sbjct: 421 RCLLYEPDPEETPNGGCPPPFSPERIGAPPVFVIGHLWSDTADTFSDKDVSEAIQGLVLK 480

Query: 481 LDEALAEQSEDLQRLGLAS-----KMKMKKT--------AAAVTAEAVHKRGEFSGCEVQ 540
           LD+AL +QS DLQRL LA+     K+K+KKT          AV A  VHK G+FSGCE+Q
Sbjct: 481 LDQALEQQSLDLQRLALANKDLEKKIKVKKTTTMNHEEKTVAVAAGTVHKVGKFSGCEIQ 540

Query: 541 LGMKRIFAALARFCWDSIAAYEELCSAM---EKQEQSQ 554
           LG+++IF  L RFC DSI AYEELC +    EKQEQSQ
Sbjct: 541 LGLRQIFVGLTRFCGDSIKAYEELCCSAITEEKQEQSQ 572

BLAST of Moc08g33510 vs. NCBI nr
Match: KAA0038463.1 (uncharacterized protein E6C27_scaffold119G00300 [Cucumis melo var. makuwa])

HSP 1 Score: 706.4 bits (1822), Expect = 1.9e-199
Identity = 404/586 (68.94%), Postives = 455/586 (77.65%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQALV +H+L DSHSAYA+SL   A+AL RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALVSTHSLIDSHSAYADSLDKTASALRRLFDQDG 60

Query: 61  --AAGDPKDS-PPAVAPSPDSSPSDSDSD--------EEEEDGSFTPEKTRSPPSPVGNF 120
               GD K + PP  AP  D S SDSDSD        EEEEDG F  EK  SP  PVG+F
Sbjct: 61  ETGGGDLKCAPPPPAAPKKDRSDSDSDSDSDSDSDSEEEEEDGCFDQEKPLSP--PVGSF 120

Query: 121 MFSNYDSARGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQK-PRTIAKK 180
           MFSNYDSARG QPPPPPPTGSSWDF NFF++YERYEQP+FNWDREG  AD+K P T    
Sbjct: 121 MFSNYDSARG-QPPPPPPTGSSWDFFNFFDSYERYEQPIFNWDREG--ADRKLPTTKVVA 180

Query: 181 KKKKKVAAVVDSKN---TKESNQLEKK-SDSKMGVSDAMRELKGSFEKASESGNSISKLI 240
           KKKKK A VVDSK    T++SNQ EKK  D KM V D MRE+KGSFEKASES NSISKL+
Sbjct: 181 KKKKKPALVVDSKKNEVTEKSNQPEKKIDDPKMSVLDLMREIKGSFEKASESSNSISKLL 240

Query: 241 SYGQRPDFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEK 300
           SYGQR  F KDTR GSRGL STLKKL VWEQKLYLEVKAEE+LRM+LEKK RQ+K+L+EK
Sbjct: 241 SYGQRMSFCKDTRTGSRGLLSTLKKLSVWEQKLYLEVKAEERLRMLLEKKCRQLKNLMEK 300

Query: 301 KADARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKS 360
           KADARK++ +R  IRNLSIKLK+SIQVVDRISI+ISKLRDEEF AE+ +LI G  SMWKS
Sbjct: 301 KADARKIDPLRNSIRNLSIKLKISIQVVDRISITISKLRDEEFFAEMNELIRGLQSMWKS 360

Query: 361 MLESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCI 420
           MLE+HKQQTQ LT+ + +E ++ NG LDD HLEAAMDLKL LENWRT F  LI TQ DCI
Sbjct: 361 MLETHKQQTQALTDPKPFE-SILNGALDDTHLEAAMDLKLELENWRTNFIELIATQKDCI 420

Query: 421 KSLNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSE 480
           K+LNGWLLRCLL+EP+PEETPNG  P PFSP RIGAPP FVI HLWS+T DKFS ++VSE
Sbjct: 421 KALNGWLLRCLLYEPDPEETPNGGCPPPFSPERIGAPPVFVIGHLWSDTADKFSDKDVSE 480

Query: 481 AICGVVAVLDEALAEQSEDLQRLGLAS-----KMKMKKT--------AAAVTAEAVHKRG 540
           AI G+V  LD+AL +QS DLQRL LA+     K+K+KKT          AV A  VHK G
Sbjct: 481 AIQGLVLKLDQALEQQSLDLQRLALANKDLEKKIKVKKTTTMNHEEKTVAVAAGTVHKVG 540

Query: 541 EFSGCEVQLGMKRIFAALARFCWDSIAAYEELCSAM---EKQEQSQ 554
           +FSGCE+QLG+++IF  L RFC DSI AYEELC +    EKQEQSQ
Sbjct: 541 KFSGCEIQLGLRQIFVGLTRFCGDSIKAYEELCCSAITEEKQEQSQ 580

BLAST of Moc08g33510 vs. NCBI nr
Match: XP_023545702.1 (nitrate regulatory gene2 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.7 bits (1794), Expect = 3.3e-196
Identity = 396/578 (68.51%), Postives = 446/578 (77.16%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQALVF+H+L DSHSAYA+SL  +A++L RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALVFTHSLIDSHSAYADSLNKVASSLRRLFDQDG 60

Query: 61  -----AAGDPK-DSPPAVAPSPDSSPSDSDSDEE---EEDGSFTPEKTRSPPSPVGNFMF 120
                  GD K   PP+ APS +   SDSDSD E   EEDG FT EK RSP   +GN MF
Sbjct: 61  ESCGGGGGDLKCPPPPSAAPSSERFDSDSDSDSESDDEEDGCFTQEKPRSP--QIGNLMF 120

Query: 121 SNYDSARGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKP---RTIAKK 180
           SNYDSARG QPPPPPPTGSSWDF NFFETYERYEQP FNWDREG   DQKP   R +  K
Sbjct: 121 SNYDSARG-QPPPPPPTGSSWDFFNFFETYERYEQPNFNWDREG--QDQKPGTTRVVTNK 180

Query: 181 KKKKKVAAVVDSKNTKESNQLEKKSD-SKMGVSDAMRELKGSFEKASESGNSISKLISYG 240
           KKK+K   VV     ++ NQ EKK D SKMGV D MRE+K SFEKASES NSISKL+SYG
Sbjct: 181 KKKQKKPPVV--APPEKLNQPEKKIDESKMGVVDLMREIKCSFEKASESSNSISKLLSYG 240

Query: 241 QRPDFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKAD 300
           QR  F KD+R  SRGL STLKKLCVWEQKLYLEVKA E+LRMILEKK RQ+K+LVEK AD
Sbjct: 241 QRMSFCKDSRTSSRGLLSTLKKLCVWEQKLYLEVKALERLRMILEKKCRQLKNLVEKNAD 300

Query: 301 ARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLE 360
            RK++SVR+ IRNLSIKL VSIQVVDRISI+ISKLRDEEFLAE+ +LIHG  SMWK+M E
Sbjct: 301 DRKIDSVRSSIRNLSIKLNVSIQVVDRISITISKLRDEEFLAEMNELIHGLQSMWKAMSE 360

Query: 361 SHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSL 420
           +HKQQTQ LT+++ +E  L NG LDD HLEAAMDLKL LENWR+ F  LITTQ DCIK+L
Sbjct: 361 AHKQQTQALTDAKPFESVL-NGALDDAHLEAAMDLKLELENWRSNFVNLITTQKDCIKAL 420

Query: 421 NGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAIC 480
           NGWLLRCLLHEPEPEETPNG  P PFSPARIGAPP FVI +LWS+T DKFS ++VSEAI 
Sbjct: 421 NGWLLRCLLHEPEPEETPNGGCPPPFSPARIGAPPVFVIAYLWSDTADKFSDKDVSEAIQ 480

Query: 481 GVVAVLDEALAEQSEDLQRLGLAS-----KMKMK--------KTAAAVTAEAVHKRGEFS 540
           GVV+ LDEAL +QS DLQRL L +     K+K K        KT AAV A  VHK G+F+
Sbjct: 481 GVVSKLDEALEQQSWDLQRLSLGNRDLEKKIKAKKMNQEIEEKTTAAVAAGTVHKVGKFN 540

Query: 541 GCEVQLGMKRIFAALARFCWDSIAAYEELCSAMEKQEQ 552
           GCE+Q G++++F  L  FC DSI AYEELCS+   Q Q
Sbjct: 541 GCEIQQGVRQVFVGLTMFCGDSITAYEELCSSAIAQSQ 570

BLAST of Moc08g33510 vs. NCBI nr
Match: XP_004144227.2 (nitrate regulatory gene2 protein isoform X1 [Cucumis sativus])

HSP 1 Score: 694.1 bits (1790), Expect = 9.7e-196
Identity = 395/590 (66.95%), Postives = 452/590 (76.61%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQAL+F+H+L DSHSAYA+SL   A+AL RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALLFTHSLIDSHSAYADSLNKTASALRRLFDQDG 60

Query: 61  --AAGDPKD-SPPAVAPSPDSSPSDSDS---------DEEEEDGSFTPEKTRSPPSPVGN 120
               GD K   PP  AP  + S SDSDS         DEEEEDG F  EK  S  SPVG+
Sbjct: 61  ETGGGDLKSPPPPPAAPKTERSDSDSDSDSDSDSDSEDEEEEDGCFGQEKPLS--SPVGS 120

Query: 121 FMFSNYDSARGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQK-PRTIAK 180
           FMFS+YD ARG QPPPPPP GSSWDF NFF++YERYEQP+FNWDREG  AD+K P T   
Sbjct: 121 FMFSSYDLARG-QPPPPPPPGSSWDFFNFFDSYERYEQPIFNWDREG--ADRKLPTTKVV 180

Query: 181 KKKKKKVAAVVDSKN---TKESNQLEKK-SDSKMGVSDAMRELKGSFEKASESGNSISKL 240
            KKKKK A VVDSK    T++SNQ EKK  D KM V D MRE+KGSFEKASES NSISKL
Sbjct: 181 AKKKKKPALVVDSKKNEVTEKSNQPEKKIDDPKMSVLDLMREIKGSFEKASESSNSISKL 240

Query: 241 ISYGQRPDFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVE 300
           +SYGQR    KDT+ GSRGL STLKKLCVWEQKLYLEVKAEE++RM+LEKK RQ+K+L+E
Sbjct: 241 LSYGQRMSICKDTKTGSRGLLSTLKKLCVWEQKLYLEVKAEERMRMVLEKKCRQLKNLME 300

Query: 301 KKADARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWK 360
           KKADARK++ +R  IRNLSIKLK+SIQVVDRISI+ISKLRDEEF AE+ +LI G  SMWK
Sbjct: 301 KKADARKIDPLRNSIRNLSIKLKISIQVVDRISITISKLRDEEFFAEMNELIRGLQSMWK 360

Query: 361 SMLESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDC 420
           SMLE+HKQQTQ LT+ + +E ++ NG LDD HLEAAMDLKL LENWRT F  LI TQ DC
Sbjct: 361 SMLETHKQQTQALTDPKPFE-SILNGALDDTHLEAAMDLKLELENWRTNFIELIATQKDC 420

Query: 421 IKSLNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVS 480
           IK+LNGWLLRCLL+EP+PEETPNG  P PFSP RIGAPP FVI HLWS+T DKFS ++VS
Sbjct: 421 IKALNGWLLRCLLYEPDPEETPNGGCPPPFSPERIGAPPVFVIGHLWSDTADKFSEKDVS 480

Query: 481 EAICGVVAVLDEALAEQSEDLQRLGLAS-----KMKMKKTAA----------AVTAEAVH 540
           EA+ G+V  LD+AL +QS DLQRL L +     K+K+KK             AV A  VH
Sbjct: 481 EAMQGLVLKLDQALEQQSLDLQRLALTNKDLEKKIKVKKKTTMNQGFEEKTMAVAAGTVH 540

Query: 541 KRGEFSGCEVQLGMKRIFAALARFCWDSIAAYEELCSAM---EKQEQSQV 555
           K G+FSGCE+QLGM++IF  L RFC DSI AYEELC +    EK+EQSQ+
Sbjct: 541 KVGKFSGCEIQLGMRQIFVGLTRFCGDSIKAYEELCCSAITEEKEEQSQI 584

BLAST of Moc08g33510 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.5e-29
Identity = 135/544 (24.82%), Postives = 223/544 (40.99%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60
           MGC  S++ +   V+ C+ R ++L   +     L+ SH+ Y  SL  + ++L     ++ 
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  A-----GDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDS 120
                   P  SPP   P     P  S   E     + T      PP P           
Sbjct: 61  PLHLHHNPPSPSPPPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPPPPP----------- 120

Query: 121 ARGQQPPPPPPTGSSWDFLNFF------ETYERYEQPVFNWDREG-GAADQKPRTIAKKK 180
                PPPPPP  S+WDF + F       + E +E+      R   G       T A   
Sbjct: 121 ----PPPPPPPPSSTWDFWDPFIPPPPSSSEEEWEEETTTATRTATGTGSDAAVTTAPTT 180

Query: 181 KKKKVAAVVD--SKNTKESNQLEKK-----SDSKMGVSDAMRELKGSFEKASESGNSISK 240
              + ++VV   SK+T  +     +     S +   + + ++E+   F KA++SG  +S 
Sbjct: 181 ATPQASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSS 240

Query: 241 LISYGQR-PDFTKDTRIG------------------SRGL-------------------- 300
           L+       DF+  ++ G                  +RG                     
Sbjct: 241 LLEISTSITDFSGHSKSGKMYSSSNYECNLNPTSFWTRGFAPSKLSEYRNAGGVIGGNCI 300

Query: 301 ----SSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIR 360
               SST+ +L  WE+KLY EVK  E ++M  EKK  QV+ L  K+A+  K E  +  + 
Sbjct: 301 VGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVE 360

Query: 361 NLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTES 420
            L  +L VS Q +   S  I KLR+ E   ++ +L+ G + MW+SM ESH+ QT ++ + 
Sbjct: 361 KLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQL 420

Query: 421 RTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEP 480
           +            ++H ++ + L+L ++ W  +F  L+  Q D I+SL GWL   L    
Sbjct: 421 KYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLRLSLFQFS 480

Query: 481 EPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAICGVVAVLDEALAE 483
           +         PL  S         +  C  W   +D+   +  SE I   +  +   +A+
Sbjct: 481 K--------NPLVRSSYE---SKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQ 518

BLAST of Moc08g33510 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.3e-22
Identity = 118/447 (26.40%), Postives = 201/447 (44.97%), Query Frame = 0

Query: 77  SSPSDSDSDEEEEDG--SFTPEKTRSPPSPVGNFMFSNYDSARGQQPPPPPPTGSSWDFL 136
           +S SD+  +EEE+D   S +   TR   S  G+ + SN      QQP P P      +  
Sbjct: 268 TSSSDAAEEEEEDDDRESISEVGTR---SEFGSTVRSNSMRRHHQQPSPMPQVYGGAEQS 327

Query: 137 NFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSKNTKESNQLEKKSDS 196
            + +     +  + +    GG      + + + +  K++   +     K +   E+ S  
Sbjct: 328 KYDKA---DDATISSGSYRGGGDIADMKMVVRHRDLKEIIDAIKENFDKAAASGEQVSQM 387

Query: 197 -KMGVSDAMRELKGSFEKASESGNSISKLIS-YGQRPDFTKDTRI---------GSRGLS 256
            ++G ++  R      +    S + +S L S +  +P      RI          S+ L 
Sbjct: 388 LELGRAELDRSFSQLKKTVIHSSSLLSNLSSTWTSKPPLAVKYRIDTTALDQPNSSKSLC 447

Query: 257 STLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNLSIK 316
           STL +L  WE+KLY E+KA E  ++  EKK  Q++    K  D  K++  +  I  L   
Sbjct: 448 STLDRLLAWEKKLYEEIKAREGFKIEHEKKLSQLQSQEYKGEDEAKLDKTKASITRLQSL 507

Query: 317 LKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRTWEL 376
           + V+ Q V   S +I +LRD + + ++ +L HGF+ MWKSM + H+ Q  ++ + R   L
Sbjct: 508 IIVTSQAVTTTSTAIIRLRDTDLVPQLVELCHGFMYMWKSMHQYHETQNSIVEQVR--GL 567

Query: 377 ALQNGK---LDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEP 436
             ++GK     ++H +A  DL+  + +W ++FS+LI  Q D I S++ W    LL   + 
Sbjct: 568 INRSGKGESTSELHRQATRDLESAVSSWHSSFSSLIKFQRDFIHSVHAWFKLTLLPVCQE 627

Query: 437 EETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAI---CGVVAVLDEALA 496
           +   +   PL           A+  C  W   +D+      SEAI     VV V+    A
Sbjct: 628 DAANHHKEPL----------DAYAFCDEWKLALDRIPDTVASEAIKSFINVVHVISAKQA 687

Query: 497 EQSEDLQRLGLASKMKMKKTAAAVTAE 505
           ++ +  +R   ASK   KK ++    E
Sbjct: 688 DEHKIKKRTESASKELEKKASSVRNLE 696


HSP 2 Score: 49.7 bits (117), Expect = 1.3e-04
Identity = 44/136 (32.35%), Postives = 60/136 (44.12%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60
           MGCA SKL N  AV  C+DR + + +A+   H LA +H+ Y  SL I  +AL       A
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSF----A 60

Query: 61  AGDP---KDSPPAV---APSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYD 120
           +G+P    D  PAV    P P  S        E+    F P +    P+P   +  S   
Sbjct: 61  SGEPLSVSDQTPAVFLHTPPPPLS--------EQSPAKFVPPRFSPSPAPSSVYPPSTSP 120

Query: 121 SARGQQPPPPPPTGSS 131
           S    + P    T S+
Sbjct: 121 SVASSKQPSVMSTSSN 124

BLAST of Moc08g33510 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.5e-15
Identity = 148/705 (20.99%), Postives = 254/705 (36.03%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60
           MGC  SK+     V  C++R + + +A+     LA +H+ Y  SL + AAAL R     A
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRF----A 60

Query: 61  AGDPK-------------DSPPAVAPSP-DSSPSDSDSDEEEEDGSFTPEKTRSPPSPVG 120
            G P               + PA+AP+P    PS + S          P+  ++PP P  
Sbjct: 61  QGHPSLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPP-- 120

Query: 121 NFMFSNYDSARGQQPPPP---------------------------------------PPT 180
                     +  QPPPP                                       P +
Sbjct: 121 -------PPTQSHQPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARSSFRKPVVGTPSS 180

Query: 181 GSSWDFLNFF-------ETYERYEQPVFNWDR-------EGGAADQKPRTIAKKKK---- 240
            S+WD+ NF+       E ++R +  +   +R       E       P  + ++ +    
Sbjct: 181 SSAWDWENFYPPSPPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDD 240

Query: 241 ----------------KKKVAAVVDSKNTKESNQLEKKSD-------------------- 300
                               A+   S+   E  ++  +S+                    
Sbjct: 241 DDEREEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAA 300

Query: 301 ------------SKMG--------------------VSDAMRELKGSFEKASESGNSISK 360
                       S+ G                    +++ +  ++  F KA+E+GN +S+
Sbjct: 301 PLPLPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEYFVKAAEAGNGVSE 360

Query: 361 LI------------------------------SYGQRPDFTKDTRIGSRGL--------- 420
           L+                              ++  +P      ++ +  L         
Sbjct: 361 LLEASRAQLDRNFRQLKKTVYHSNSLLSSLSSTWTSKPPLAVRYKLDTNALEMESMEGKS 420

Query: 421 -SSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNLS 480
             STL++L  WE+KLY EVKA E +++  EKK   ++ L  +  D+ K++  +  I  L 
Sbjct: 421 HGSTLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKASINKLQ 480

Query: 481 IKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRTW 522
             + V+ Q     S +I ++RD E   ++ +L    LSMW+SM   H+ Q +++ + R  
Sbjct: 481 SLIIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEIVQQVRGL 540

BLAST of Moc08g33510 vs. ExPASy TrEMBL
Match: A0A6J1DI33 (uncharacterized protein LOC111020693 OS=Momordica charantia OX=3673 GN=LOC111020693 PE=4 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 1.1e-234
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60
           MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA
Sbjct: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60

Query: 61  AGDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSARGQQ 120
           AGDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSARGQQ
Sbjct: 61  AGDPKDSPPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSARGQQ 120

Query: 121 PPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSK 180
           PPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSK
Sbjct: 121 PPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKPRTIAKKKKKKKVAAVVDSK 180

Query: 181 NTKESNQLEKKSDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDFTKDTRIGSR 240
           NTKESNQLEKKSDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDFTKDTRIGSR
Sbjct: 181 NTKESNQLEKKSDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDFTKDTRIGSR 240

Query: 241 GLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNL 300
           GLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNL
Sbjct: 241 GLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNL 300

Query: 301 SIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRT 360
           SIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRT
Sbjct: 301 SIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRT 360

Query: 361 WELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEP 420
           WELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEP
Sbjct: 361 WELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEP 420

Query: 421 EETPN 426
           EETPN
Sbjct: 421 EETPN 425

BLAST of Moc08g33510 vs. ExPASy TrEMBL
Match: A0A1S3CP29 (uncharacterized protein LOC103502634 OS=Cucumis melo OX=3656 GN=LOC103502634 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 8.3e-201
Identity = 402/578 (69.55%), Postives = 454/578 (78.55%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQALV +H+L DSHSAYA+SL   A+AL RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALVSTHSLIDSHSAYADSLDKTASALRRLFDQDG 60

Query: 61  --AAGDPKDS-PPAVAPSPDSSPSDSDSDEEEEDGSFTPEKTRSPPSPVGNFMFSNYDSA 120
               GD K + PP  AP  D S SDSDS+EEEEDG F  EK  SP  PVG+FMFSNYDSA
Sbjct: 61  ETGGGDLKCAPPPPAAPKKDRSDSDSDSEEEEEDGCFDQEKPLSP--PVGSFMFSNYDSA 120

Query: 121 RGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQK-PRTIAKKKKKKKVAA 180
           RG QPPPPPPTGSSWDF NFF++YERYEQP+FNWDREG  AD+K P T    KKKKK A 
Sbjct: 121 RG-QPPPPPPTGSSWDFFNFFDSYERYEQPIFNWDREG--ADRKLPTTKVVAKKKKKPAL 180

Query: 181 VVDSKN---TKESNQLEKK-SDSKMGVSDAMRELKGSFEKASESGNSISKLISYGQRPDF 240
           VVDSK    T++SNQ EKK  D KM V D MRE+KGSFEKASES NSISKL+SYGQR  F
Sbjct: 181 VVDSKKNEVTEKSNQPEKKIDDPKMSVLDLMREIKGSFEKASESSNSISKLLSYGQRMSF 240

Query: 241 TKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVE 300
            KDTR GSRGL STLKKL VWEQKLYLEVKAEE+LRM+LEKK RQ+K+L+EKKADARK++
Sbjct: 241 CKDTRTGSRGLLSTLKKLSVWEQKLYLEVKAEERLRMLLEKKCRQLKNLMEKKADARKID 300

Query: 301 SVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQ 360
            +R  IRNLSIKLK+SIQVVDRISI+ISKLRDEEF AE+ +LI G  SMWKSMLE+HKQQ
Sbjct: 301 PLRNSIRNLSIKLKISIQVVDRISITISKLRDEEFFAEMNELIRGLQSMWKSMLETHKQQ 360

Query: 361 TQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLL 420
           TQ LT+ + +E ++ NG LDD HLEAAMDLKL LENWRT F  LI TQ DCIK+LNGWLL
Sbjct: 361 TQALTDPKPFE-SILNGALDDTHLEAAMDLKLELENWRTNFIELIATQKDCIKALNGWLL 420

Query: 421 RCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAICGVVAV 480
           RCLL+EP+PEETPNG  P PFSP RIGAPP FVI HLWS+T D FS ++VSEAI G+V  
Sbjct: 421 RCLLYEPDPEETPNGGCPPPFSPERIGAPPVFVIGHLWSDTADTFSDKDVSEAIQGLVLK 480

Query: 481 LDEALAEQSEDLQRLGLAS-----KMKMKKT--------AAAVTAEAVHKRGEFSGCEVQ 540
           LD+AL +QS DLQRL LA+     K+K+KKT          AV A  VHK G+FSGCE+Q
Sbjct: 481 LDQALEQQSLDLQRLALANKDLEKKIKVKKTTTMNHEEKTVAVAAGTVHKVGKFSGCEIQ 540

Query: 541 LGMKRIFAALARFCWDSIAAYEELCSAM---EKQEQSQ 554
           LG+++IF  L RFC DSI AYEELC +    EKQEQSQ
Sbjct: 541 LGLRQIFVGLTRFCGDSIKAYEELCCSAITEEKQEQSQ 572

BLAST of Moc08g33510 vs. ExPASy TrEMBL
Match: A0A5A7T6A4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00300 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 9.2e-200
Identity = 404/586 (68.94%), Postives = 455/586 (77.65%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQALV +H+L DSHSAYA+SL   A+AL RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALVSTHSLIDSHSAYADSLDKTASALRRLFDQDG 60

Query: 61  --AAGDPKDS-PPAVAPSPDSSPSDSDSD--------EEEEDGSFTPEKTRSPPSPVGNF 120
               GD K + PP  AP  D S SDSDSD        EEEEDG F  EK  SP  PVG+F
Sbjct: 61  ETGGGDLKCAPPPPAAPKKDRSDSDSDSDSDSDSDSEEEEEDGCFDQEKPLSP--PVGSF 120

Query: 121 MFSNYDSARGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQK-PRTIAKK 180
           MFSNYDSARG QPPPPPPTGSSWDF NFF++YERYEQP+FNWDREG  AD+K P T    
Sbjct: 121 MFSNYDSARG-QPPPPPPTGSSWDFFNFFDSYERYEQPIFNWDREG--ADRKLPTTKVVA 180

Query: 181 KKKKKVAAVVDSKN---TKESNQLEKK-SDSKMGVSDAMRELKGSFEKASESGNSISKLI 240
           KKKKK A VVDSK    T++SNQ EKK  D KM V D MRE+KGSFEKASES NSISKL+
Sbjct: 181 KKKKKPALVVDSKKNEVTEKSNQPEKKIDDPKMSVLDLMREIKGSFEKASESSNSISKLL 240

Query: 241 SYGQRPDFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEK 300
           SYGQR  F KDTR GSRGL STLKKL VWEQKLYLEVKAEE+LRM+LEKK RQ+K+L+EK
Sbjct: 241 SYGQRMSFCKDTRTGSRGLLSTLKKLSVWEQKLYLEVKAEERLRMLLEKKCRQLKNLMEK 300

Query: 301 KADARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKS 360
           KADARK++ +R  IRNLSIKLK+SIQVVDRISI+ISKLRDEEF AE+ +LI G  SMWKS
Sbjct: 301 KADARKIDPLRNSIRNLSIKLKISIQVVDRISITISKLRDEEFFAEMNELIRGLQSMWKS 360

Query: 361 MLESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCI 420
           MLE+HKQQTQ LT+ + +E ++ NG LDD HLEAAMDLKL LENWRT F  LI TQ DCI
Sbjct: 361 MLETHKQQTQALTDPKPFE-SILNGALDDTHLEAAMDLKLELENWRTNFIELIATQKDCI 420

Query: 421 KSLNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSE 480
           K+LNGWLLRCLL+EP+PEETPNG  P PFSP RIGAPP FVI HLWS+T DKFS ++VSE
Sbjct: 421 KALNGWLLRCLLYEPDPEETPNGGCPPPFSPERIGAPPVFVIGHLWSDTADKFSDKDVSE 480

Query: 481 AICGVVAVLDEALAEQSEDLQRLGLAS-----KMKMKKT--------AAAVTAEAVHKRG 540
           AI G+V  LD+AL +QS DLQRL LA+     K+K+KKT          AV A  VHK G
Sbjct: 481 AIQGLVLKLDQALEQQSLDLQRLALANKDLEKKIKVKKTTTMNHEEKTVAVAAGTVHKVG 540

Query: 541 EFSGCEVQLGMKRIFAALARFCWDSIAAYEELCSAM---EKQEQSQ 554
           +FSGCE+QLG+++IF  L RFC DSI AYEELC +    EKQEQSQ
Sbjct: 541 KFSGCEIQLGLRQIFVGLTRFCGDSIKAYEELCCSAITEEKQEQSQ 580

BLAST of Moc08g33510 vs. ExPASy TrEMBL
Match: A0A0A0KGR7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G358160 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 6.4e-193
Identity = 393/590 (66.61%), Postives = 449/590 (76.10%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQAL+F+H+L DSHSAYA+SL   A+AL RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALLFTHSLIDSHSAYADSLNKTASALRRLFDQDG 60

Query: 61  --AAGDPKD-SPPAVAPSPDSSPSDSDS---------DEEEEDGSFTPEKTRSPPSPVGN 120
               GD K   PP  AP  + S SDSDS         DEEEEDG F  EK  S  SPVG+
Sbjct: 61  ETGGGDLKSPPPPPAAPKTERSDSDSDSDSDSDSDSEDEEEEDGCFGQEKPLS--SPVGS 120

Query: 121 FMFSNYDSARGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQK-PRTIAK 180
           FMFS+YD ARG QPPPPPP GSSWDF NFF++YERYEQP+FNWDREG  AD+K P T   
Sbjct: 121 FMFSSYDLARG-QPPPPPPPGSSWDFFNFFDSYERYEQPIFNWDREG--ADRKLPTTKVV 180

Query: 181 KKKKKKVAAVVDSKN---TKESNQLEKK-SDSKMGVSDAMRELKGSFEKASESGNSISKL 240
            KKKKK A VVDSK    T++SNQ EKK  D KM V D MRE+KGSFEKASES NSISKL
Sbjct: 181 AKKKKKPALVVDSKKNEVTEKSNQPEKKIDDPKMSVLDLMREIKGSFEKASESSNSISKL 240

Query: 241 ISYGQRPDFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVE 300
           +SYGQR    K    GSRGL STLKKLCVWEQKLYLEVKAEE++RM+LEKK RQ+K+L+E
Sbjct: 241 LSYGQRMSICK----GSRGLLSTLKKLCVWEQKLYLEVKAEERMRMVLEKKCRQLKNLME 300

Query: 301 KKADARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWK 360
           KKADARK++ +R  IRNLSIKLK+SIQVVDRISI+ISKLRDEEF AE+ +LI G  SMWK
Sbjct: 301 KKADARKIDPLRNSIRNLSIKLKISIQVVDRISITISKLRDEEFFAEMNELIRGLQSMWK 360

Query: 361 SMLESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDC 420
           SMLE+HKQQTQ LT+ + +E ++ NG LDD HLEAAMDLKL LENWRT F  LI TQ DC
Sbjct: 361 SMLETHKQQTQALTDPKPFE-SILNGALDDTHLEAAMDLKLELENWRTNFIELIATQKDC 420

Query: 421 IKSLNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVS 480
           IK+LNGWLLRCLL+EP+PEETPNG  P PFSP RIGAPP FVI HLWS+T DKFS ++VS
Sbjct: 421 IKALNGWLLRCLLYEPDPEETPNGGCPPPFSPERIGAPPVFVIGHLWSDTADKFSEKDVS 480

Query: 481 EAICGVVAVLDEALAEQSEDLQRLGLAS-----KMKMKKTAA----------AVTAEAVH 540
           EA+ G+V  LD+AL +QS DLQRL L +     K+K+KK             AV A  VH
Sbjct: 481 EAMQGLVLKLDQALEQQSLDLQRLALTNKDLEKKIKVKKKTTMNQGFEEKTMAVAAGTVH 540

Query: 541 KRGEFSGCEVQLGMKRIFAALARFCWDSIAAYEELCSAM---EKQEQSQV 555
           K G+FSGCE+QLGM++IF  L RFC DSI AYEELC +    EK+EQSQ+
Sbjct: 541 KVGKFSGCEIQLGMRQIFVGLTRFCGDSIKAYEELCCSAITEEKEEQSQI 580

BLAST of Moc08g33510 vs. ExPASy TrEMBL
Match: A0A6J1G2P6 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111450252 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 6.4e-193
Identity = 390/580 (67.24%), Postives = 442/580 (76.21%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQD- 60
           MGCA+SKL NLPAVALCRDRCKFLDQALVF+H+L DSHSAYA+SL  +A+AL RLFDQD 
Sbjct: 1   MGCASSKLDNLPAVALCRDRCKFLDQALVFTHSLIDSHSAYADSLNKVASALRRLFDQDG 60

Query: 61  -----AAGDPK-DSPPAVAPSPDSSPSDSDSD-----EEEEDGSFTPEKTRSPPSPVGNF 120
                  GD K   PP+ A S + S SDSDSD     ++EEDG FT EK RSP   +GN 
Sbjct: 61  ESCGGGGGDLKCPPPPSAATSSERSDSDSDSDSDSESDDEEDGCFTQEKPRSP--QIGNL 120

Query: 121 MFSNYDSARGQQPPPPPPTGSSWDFLNFFETYERYEQPVFNWDREGGAADQKP---RTIA 180
           MFSNYDSARG QPPPPPPTGSSWDF NFFETYERYEQP FNWD      DQKP   R + 
Sbjct: 121 MFSNYDSARG-QPPPPPPTGSSWDFFNFFETYERYEQPNFNWD-----PDQKPGTTRVVT 180

Query: 181 KKKKKKKVAAVVDSKNTKESNQLEKKSD-SKMGVSDAMRELKGSFEKASESGNSISKLIS 240
            KKKK+K   VV     ++ NQ EKK D SKMGV D M E+KGSFEKASES NSISKL+S
Sbjct: 181 NKKKKQKKPPVV--APPEKLNQPEKKIDESKMGVVDLMTEIKGSFEKASESSNSISKLLS 240

Query: 241 YGQRPDFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKK 300
           YGQR  F KD+R  SRGL STLKKLCVWEQKLYLEVK  E+LRMILEKK RQ+K+LVEK 
Sbjct: 241 YGQRMSFCKDSRTSSRGLLSTLKKLCVWEQKLYLEVKGLERLRMILEKKCRQLKNLVEKN 300

Query: 301 ADARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSM 360
           AD RK++SVR+ IRNLSIKL VSIQVVDRISI+ISKLRDEEFLAE+ +LIHG  SMWK+M
Sbjct: 301 ADDRKIDSVRSSIRNLSIKLNVSIQVVDRISITISKLRDEEFLAEMNELIHGLQSMWKAM 360

Query: 361 LESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIK 420
            E+HKQQTQ LT+++ +E  L NG LDD HLEAAMDLKL LENWR+ F  LI TQ DCIK
Sbjct: 361 SEAHKQQTQALTDAKPFESVL-NGALDDAHLEAAMDLKLELENWRSNFVNLIATQKDCIK 420

Query: 421 SLNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEA 480
           +LNGWLLRCLLHEPEPEETPNG  P PFSPARIGAPP FVI +LWS+T +KFS ++VSEA
Sbjct: 421 ALNGWLLRCLLHEPEPEETPNGGCPPPFSPARIGAPPVFVIAYLWSDTAEKFSDKDVSEA 480

Query: 481 ICGVVAVLDEALAEQSEDLQRLGLAS-----KMKMK--------KTAAAVTAEAVHKRGE 540
           I GVV+ LDEAL +QS DLQRL L +     K+K K        KT AAV A  VHK G+
Sbjct: 481 IQGVVSKLDEALEQQSWDLQRLALGNRDLEKKIKAKKMNQEIEEKTTAAVAAGTVHKVGK 540

Query: 541 FSGCEVQLGMKRIFAALARFCWDSIAAYEELCSAMEKQEQ 552
           F+GCE+Q G++++   L RFC DSI AYEELCS+   Q Q
Sbjct: 541 FNGCEIQQGVRQVLVGLTRFCGDSITAYEELCSSAIAQSQ 569

BLAST of Moc08g33510 vs. TAIR 10
Match: AT2G17110.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 265.8 bits (678), Expect = 7.9e-71
Identity = 219/728 (30.08%), Postives = 337/728 (46.29%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQDA 60
           MGC+TSKL +LPAVALCRDRC FL+ A+   +AL+++H +Y +SL  ++ +LH+  +   
Sbjct: 1   MGCSTSKLDDLPAVALCRDRCSFLEAAIHQRYALSEAHVSYTQSLKAISHSLHQFINHHH 60

Query: 61  AGDPKDSPPAVAPSPDSSPS----DSDSDEEEED-------------------------- 120
             +  DSP    P  DS       DSDSD +++D                          
Sbjct: 61  RYNDSDSPKKAKPKMDSGSGHLDFDSDSDSDDDDDIDSLHSSPLHHHLEDDDSNPKSYLH 120

Query: 121 -----GSFTP------------------EKTRSPPSPVGNFMFSNYDSARGQQPPPPPPT 180
                 S+ P                  E + S  S    ++ SNY S +   PPP PP 
Sbjct: 121 MNYMKNSYMPPSLVYEQRPSSPQRVHFGESSSSSTSEYNPYLNSNYGS-KLPPPPPSPPR 180

Query: 181 GSSWDFLNFFETY-----------------------------------------ERYEQP 240
              WDFL+ F+TY                                            E+P
Sbjct: 181 EKVWDFLDPFDTYYTPYTPSRDTRELRDELGVPDLEEDDVVVKEVHGKQKFVAAVSVEEP 240

Query: 241 VFNWDRE-------GGAA---DQKPRTIAKKKKKKKVAAVVDSKNTKESNQLE-KKSDS- 300
           + N           GG A     +P    +K++ +    +V+ K  ++S   E +KS + 
Sbjct: 241 LGNSGASTSGGGGGGGKASLYQTRPSVSVEKEEMEHEVHIVEKKIVEDSGGDEVRKSKAA 300

Query: 301 --------KMGVSDAMRELKGSFEKASESGNSISKLISYGQRP----------------- 360
                   + GV +  +E++  F +A+ESGN I+ ++  G+ P                 
Sbjct: 301 VARGGGGVRRGVPEVAKEIEAQFLRAAESGNEIAVMLEVGKHPYGRKNVSSKKLYEGTPS 360

Query: 361 -----------------------------DFTKDTRIGSRGLSSTLKKLCVWEQKLYLEV 420
                                        D   +  + SR LSSTL KL +WE+KLY EV
Sbjct: 361 PSVVSSAQSSTSKKAKAEASSSVTAPTYADIEAELALKSRNLSSTLHKLHLWEKKLYDEV 420

Query: 421 KAEEKLRMILEKKWRQVKHLVEKKADARKVESVRTLIRNLSIKLKVSIQVVDRISISISK 480
           KAEEK+R+  EKK R++K + E+ A+ +KV+S R L+R+LS K++++IQVVD+IS++I+K
Sbjct: 421 KAEEKMRVNHEKKLRKLKRMDERGAENQKVDSTRKLVRSLSTKIRIAIQVVDKISVTINK 480

Query: 481 LRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMD 540
           +RDEE   ++ +LI G   MWKSMLE HK Q + + E+R       +      HLE    
Sbjct: 481 IRDEELWLQLNELIQGLSKMWKSMLECHKSQCEAIKEARGLGPIRASKNFGGEHLEVTRT 540

Query: 541 LKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAP 551
           L   L NW   FS+ ++ Q   ++ LN WL++CL +  EPEETP+G   +PFSP RIGAP
Sbjct: 541 LGYELINWIVGFSSWVSAQKGFVRELNSWLMKCLFY--EPEETPDGI--VPFSPGRIGAP 600

BLAST of Moc08g33510 vs. TAIR 10
Match: AT1G20530.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 257.3 bits (656), Expect = 2.8e-68
Identity = 206/623 (33.07%), Postives = 312/623 (50.08%), Query Frame = 0

Query: 1   MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALHRLFDQ-- 60
           MGC+ SKL  LPAV+LCRDRC  L+  L  S+ALAD+HSAY  SL  +  ALHR F    
Sbjct: 1   MGCSPSKLDGLPAVSLCRDRCSSLEDTLRRSYALADAHSAYLLSLNTVGPALHRFFQHAV 60

Query: 61  ----DAAGDPKDSPPAVAPSPDSS-----------PSDSDSDEEEEDGSFTPEKTRSPPS 120
               D   +  +SP   +P   SS           P   DSD EE +G+     + S P 
Sbjct: 61  ESPPDVESEADESPETYSPESCSSAHSVTSSDSDLPPKFDSDCEEGEGTNCDLLSGSKPE 120

Query: 121 PVG----NFMFSNYDSARGQQPPPPP-PTGSSWDFLNFFETYE-RYEQPVFNW-DREGGA 180
            +     +F    Y+S     PPPPP P+  +WDF+NFFE+YE  Y   V +  DRE   
Sbjct: 121 SLNLNHDSFYSRRYESGTITPPPPPPAPSNYAWDFINFFESYELPYTTDVNDLKDRETTP 180

Query: 181 ADQKPR------TIAKKKKKKKV-----AAVVDSKNTKESNQLEKKSDSKMGVSDAMREL 240
           + ++ +      T+++  +K KV        +  KN K + +  K       +S+  ++L
Sbjct: 181 SHEEDKLKKKKITVSQNDEKIKVEEEKKTLRISEKNRKRAPKESKDQKVSSDLSEVTKQL 240

Query: 241 KGSFEKASESGNSISKLIS------YGQRPDFTKDTRI---------------------- 300
           +  F+KASESGN +SK+        Y +   +  + RI                      
Sbjct: 241 QEMFKKASESGNDVSKMFDTSRFRYYQKSSVYQCNVRILLPSSNILYTKKVMTPFDPKPV 300

Query: 301 ---GSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVESVR 360
                  LSSTLKKL +WE+KLY EVKAEEKLR    K ++ ++ L  K AD  K+E++R
Sbjct: 301 EESNFNNLSSTLKKLFMWEKKLYQEVKAEEKLRTSHMKNYKLLRRLEAKSADLSKIEAIR 360

Query: 361 TLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQTQV 420
           + I+ LS +++VSI  ++ I ++I+KLRDEE   ++ +LIH    MW SMLE H +Q++V
Sbjct: 361 SSIQCLSTRMRVSIHKINNICLTINKLRDEELWFQMKELIHRLSEMWNSMLECHSRQSKV 420

Query: 421 LTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLLRCL 480
           + E++  +       LD   LE AM+LKL L NW  + S  I  Q   +K+LN WL+RCL
Sbjct: 421 IAEAKKLDKMTIKENLDLSQLELAMELKLELRNWSLSMSNWIDAQAQYVKALNNWLMRCL 480

Query: 481 LHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGE-EVSEAICGVVAVLD 540
             EP+ E TP+ +            PP F   + WS+ ++   GE E +EA+  ++  ++
Sbjct: 481 KQEPQ-EPTPDLS----------EEPPLFGAINTWSQNLEISHGEKEFTEAVYTILMHVN 540

Query: 541 EALAEQSEDLQ--------------RLGLASKMKMKKTAAAVTAEAVHKRGEFSGCEVQL 543
             + +Q  +L+              +L +  K + K      T  +V   G  +   ++ 
Sbjct: 541 HQVEKQRMELEEQRNVNGSVKDIERKLMMLEKEEQKMQRKMKTVPSVALMGSLN---LKS 600

BLAST of Moc08g33510 vs. TAIR 10
Match: AT4G35240.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 219.9 bits (559), Expect = 5.0e-57
Identity = 157/465 (33.76%), Postives = 244/465 (52.47%), Query Frame = 0

Query: 153 EGGAADQKPRTIAKKKKKKKVAAVVDSKNTKESNQLEKKSDSKMG--------VSDAMRE 212
           +  A   +P    +K+  +    VV+ K  ++  +    + ++ G        V +  +E
Sbjct: 368 DASAYQSRPSVSVEKEGMEYEVHVVEKKVVEDEERRSNATATRGGGGGGGPRAVPEVAKE 427

Query: 213 LKGSFEKASESGNSISKLISYGQRP----------------------------------- 272
           ++  F KA+ESG+ I+KL+  G+ P                                   
Sbjct: 428 IENQFVKAAESGSEIAKLLEVGKHPYGRKHAASKMLHGVTPSLPSTSGGTSSSAAAAVVP 487

Query: 273 ----DFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKA 332
               D  ++    SR LSSTL KL +WE+KLY EVKAEEKLR+  EKK R++K L ++ A
Sbjct: 488 PTYADIEEELASRSRNLSSTLHKLHLWEKKLYHEVKAEEKLRLAHEKKLRKLKRLDQRGA 547

Query: 333 DARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSML 392
           +A KV+  R L+R++S K++++IQVVD+IS++I+K+RDE+   ++  LI G   MWK+ML
Sbjct: 548 EAIKVDKTRKLVRDMSTKIRIAIQVVDKISVTINKIRDEDLWPQLNALIQGLTRMWKTML 607

Query: 393 ESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKS 452
           E H+ Q Q + E++       + KL D HLEA   L   L NW   FS+ ++ Q   +K 
Sbjct: 608 ECHQSQCQAIREAQGLGPIRASKKLGDEHLEATSLLGHELINWILGFSSWVSAQKGYVKE 667

Query: 453 LNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAI 512
           LN WL++CLL+  EPEETP+G   +PFSP RIGAPP FVIC+ WS+ +D+ S +EV EA+
Sbjct: 668 LNKWLMKCLLY--EPEETPDGI--VPFSPGRIGAPPIFVICNQWSQALDRISEKEVIEAM 727

Query: 513 CG-VVAVLD---------------------EALAEQSEDLQRLGLASKMKMKKTA----- 543
                +VL                        +  + + +QR   A + KM   A     
Sbjct: 728 RSFTTSVLQLWEQDRLDTMMTGHGDSEKKVRNMDREEQRIQREIQALEKKMILVAPGDGN 787


HSP 2 Score: 62.8 bits (151), Expect = 1.0e-09
Identity = 30/53 (56.60%), Postives = 39/53 (73.58%), Query Frame = 0

Query: 1  MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALH 54
          MGC +SKL +LPAVALCR+RC FL+ A+   +ALA+SH AY  SL  +  +LH
Sbjct: 1  MGCTSSKLDDLPAVALCRERCAFLEAAIHQRYALAESHVAYTHSLREIGHSLH 53

BLAST of Moc08g33510 vs. TAIR 10
Match: AT4G35240.2 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 219.9 bits (559), Expect = 5.0e-57
Identity = 157/465 (33.76%), Postives = 244/465 (52.47%), Query Frame = 0

Query: 153 EGGAADQKPRTIAKKKKKKKVAAVVDSKNTKESNQLEKKSDSKMG--------VSDAMRE 212
           +  A   +P    +K+  +    VV+ K  ++  +    + ++ G        V +  +E
Sbjct: 368 DASAYQSRPSVSVEKEGMEYEVHVVEKKVVEDEERRSNATATRGGGGGGGPRAVPEVAKE 427

Query: 213 LKGSFEKASESGNSISKLISYGQRP----------------------------------- 272
           ++  F KA+ESG+ I+KL+  G+ P                                   
Sbjct: 428 IENQFVKAAESGSEIAKLLEVGKHPYGRKHAASKMLHGVTPSLPSTSGGTSSSAAAAVVP 487

Query: 273 ----DFTKDTRIGSRGLSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKA 332
               D  ++    SR LSSTL KL +WE+KLY EVKAEEKLR+  EKK R++K L ++ A
Sbjct: 488 PTYADIEEELASRSRNLSSTLHKLHLWEKKLYHEVKAEEKLRLAHEKKLRKLKRLDQRGA 547

Query: 333 DARKVESVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSML 392
           +A KV+  R L+R++S K++++IQVVD+IS++I+K+RDE+   ++  LI G   MWK+ML
Sbjct: 548 EAIKVDKTRKLVRDMSTKIRIAIQVVDKISVTINKIRDEDLWPQLNALIQGLTRMWKTML 607

Query: 393 ESHKQQTQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKS 452
           E H+ Q Q + E++       + KL D HLEA   L   L NW   FS+ ++ Q   +K 
Sbjct: 608 ECHQSQCQAIREAQGLGPIRASKKLGDEHLEATSLLGHELINWILGFSSWVSAQKGYVKE 667

Query: 453 LNGWLLRCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAI 512
           LN WL++CLL+  EPEETP+G   +PFSP RIGAPP FVIC+ WS+ +D+ S +EV EA+
Sbjct: 668 LNKWLMKCLLY--EPEETPDGI--VPFSPGRIGAPPIFVICNQWSQALDRISEKEVIEAM 727

Query: 513 CG-VVAVLD---------------------EALAEQSEDLQRLGLASKMKMKKTA----- 543
                +VL                        +  + + +QR   A + KM   A     
Sbjct: 728 RSFTTSVLQLWEQDRLDTMMTGHGDSEKKVRNMDREEQRIQREIQALEKKMILVAPGDGN 787


HSP 2 Score: 62.8 bits (151), Expect = 1.0e-09
Identity = 30/53 (56.60%), Postives = 39/53 (73.58%), Query Frame = 0

Query: 1  MGCATSKLHNLPAVALCRDRCKFLDQALVFSHALADSHSAYAESLTIMAAALH 54
          MGC +SKL +LPAVALCR+RC FL+ A+   +ALA+SH AY  SL  +  +LH
Sbjct: 1  MGCTSSKLDDLPAVALCRERCAFLEAAIHQRYALAESHVAYTHSLREIGHSLH 53

BLAST of Moc08g33510 vs. TAIR 10
Match: AT1G21740.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 203.8 bits (517), Expect = 3.7e-52
Identity = 115/251 (45.82%), Postives = 156/251 (62.15%), Query Frame = 0

Query: 233 KDTRIGSRG-LSSTLKKLCVWEQKLYLEVKAEEKLRMILEKKWRQVKHLVEKKADARKVE 292
           +D R G  G LS+TL++L  WE+KLY EVK EEKLR++ E+K R +K L    A++ K++
Sbjct: 580 QDVREGLTGNLSATLEQLYAWEKKLYKEVKDEEKLRVVYEEKCRTLKKLDSLGAESSKID 639

Query: 293 SVRTLIRNLSIKLKVSIQVVDRISISISKLRDEEFLAEITQLIHGFLSMWKSMLESHKQQ 352
           + R  IR L  KL V I+ VD IS  I KLRDEE   ++TQLIHG + MW+SML+ H++Q
Sbjct: 640 TTRAAIRKLLTKLDVCIRSVDSISSRIHKLRDEELQPQLTQLIHGLIRMWRSMLKCHQKQ 699

Query: 353 TQVLTESRTWELALQNGKLDDIHLEAAMDLKLHLENWRTAFSTLITTQNDCIKSLNGWLL 412
            Q + ES+   L    G   D  L+A +DL++ L  W  +F+  + TQ   ++SLNGWL 
Sbjct: 700 FQAIMESKVRSLRANTGLQRDSGLKAILDLEMELREWCISFNDWVNTQKSYVESLNGWLS 759

Query: 413 RCLLHEPEPEETPNGAGPLPFSPARIGAPPAFVICHLWSETVDKFSGEEVSEAICGVVAV 472
           RCL +EPE  E     G  PFSP+R+GAP  FVIC  W E + + SGE VS A+ G  + 
Sbjct: 760 RCLHYEPESTED----GIAPFSPSRVGAPQVFVICKDWQEAMARISGENVSNAMQGFASS 819

Query: 473 LDEALAEQSED 483
           L E    Q E+
Sbjct: 820 LHELWERQDEE 826

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153114.12.4e-234100.00uncharacterized protein LOC111020693, partial [Momordica charantia][more]
XP_008464870.11.7e-20069.55PREDICTED: uncharacterized protein LOC103502634 [Cucumis melo][more]
KAA0038463.11.9e-19968.94uncharacterized protein E6C27_scaffold119G00300 [Cucumis melo var. makuwa][more]
XP_023545702.13.3e-19668.51nitrate regulatory gene2 protein-like [Cucurbita pepo subsp. pepo][more]
XP_004144227.29.7e-19666.95nitrate regulatory gene2 protein isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A178VBJ02.5e-2924.82Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q93YU82.3e-2226.40Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Q9AQW13.5e-1520.99Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
Match NameE-valueIdentityDescription
A0A6J1DI331.1e-234100.00uncharacterized protein LOC111020693 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A1S3CP298.3e-20169.55uncharacterized protein LOC103502634 OS=Cucumis melo OX=3656 GN=LOC103502634 PE=... [more]
A0A5A7T6A49.2e-20068.94Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A0A0KGR76.4e-19366.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G358160 PE=4 SV=1[more]
A0A6J1G2P66.4e-19367.24nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111450... [more]
Match NameE-valueIdentityDescription
AT2G17110.17.9e-7130.08Protein of unknown function (DUF630 and DUF632) [more]
AT1G20530.12.8e-6833.07Protein of unknown function (DUF630 and DUF632) [more]
AT4G35240.15.0e-5733.76Protein of unknown function (DUF630 and DUF632) [more]
AT4G35240.25.0e-5733.76Protein of unknown function (DUF630 and DUF632) [more]
AT1G21740.13.7e-5245.82Protein of unknown function (DUF630 and DUF632) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 233..472
e-value: 5.9E-75
score: 252.6
IPR006868Domain of unknown function DUF630PFAMPF04783DUF630coord: 1..58
e-value: 3.5E-19
score: 68.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..200
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..200
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..117
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 491..553
coord: 230..491
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..228
NoneNo IPR availablePANTHERPTHR21450:SF19F5M15.15coord: 1..228
coord: 491..553
coord: 230..491

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g33510.1Moc08g33510.1mRNA