Moc08g33220 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g33220
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionTranscription factor MYB34
Locationchr8: 24116555 .. 24117460 (+)
RNA-Seq ExpressionMoc08g33220
SyntenyMoc08g33220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGATCTGCAAAGGCTAGAGTATCTACTTGACAATGAGATTGAATGTACATTTCCTAGGAGAATAAGGGAGCAACGTAGACAGCAAGTGGAAACTAACCCAATGGATCAATTACCAAGACTCCCTAATCCAATGGTTGAAGTAGTTGTACCAGCAGCTGCTCCCACTCTTAACACCATTCTATTGGCAGATGATGGAGAAAGGGCTATCAGAGCCTATGCTGCACTAGTACTTCATAGTTTTCATCCAGTTATAGCAGGTCCAGAGATAGAAGCTGAGAGGTTTGAATTAAAACCAGTTATGTTCCAGATGCTTCAAACAGTGGGGAAATTCTTTGGAAATCCATGTGAGGACTCCCATCTGCACTTGAGGTATTTTCTGGAAGTAAGTGACTCATTCAAGATGTAAGGAGTATCCAAGGAAGCATTGCATTTGAAATTGTTTCCATACTCGCTGAGAGACAAGGTAAGAGACTGGTTAAACTCTATGCCAGCTGAGTCCATTACATCTTGGAATGATCTTGCTGAGAAGTTTTTAATGAAATACTTTCCCCCAAGTAAGAATGTCCAAGTATCGAGGGGACATTAATAATTTCCAGCAGAGGCATGTGAATCTGTGAGCGAATCATGGGAAAGGTTCAAGGAATTGTTAAGGAGATGCCCATACCACGGTTTGCCAAGGTGTATCCAGATAAAGAACTTCTACAATGGGTTGAGTGAAGCAACACGCCTGGTAGTTGATGCATCTGCTAATAGAGCATTGTTGTCTAAGTCGTATGTAGAAGCAATTGACATATTGGAAAGGATTTCTGCTAATAACTACCACTGGTCAGATTCCAGAGCTGATAGGAGCAATCATGGAGTTAATGATAATGAGGCAATGGCTGCACTTAATTTTCTATAG

mRNA sequence

ATGCATGATCTGCAAAGGCTAGAGTATCTACTTGACAATGAGATTGAATGTACATTTCCTAGGAGAATAAGGGAGCAACGTAGACAGCAAGTGGAAACTAACCCAATGGATCAATTACCAAGACTCCCTAATCCAATGGTTGAAGTAGTTGTACCAGCAGCTGCTCCCACTCTTAACACCATTCTATTGGCAGATGATGGAGAAAGGGCTATCAGAGCCTATGCTGCACTAGTACTTCATAGTTTTCATCCAGTTATAGCAGGTCCAGAGATAGAAGCTGAGAGGTTTGAATTAAAACCAGTTATGTTCCAGATGCTTCAAACAGTGGGGAAATTCTTTGGAAATCCATGTGAGGACTCCCATCTGCACTTGAGGTATTTTCTGGAAATAAAGAACTTCTACAATGGGTTGAGTGAAGCAACACGCCTGGTAGTTGATGCATCTGCTAATAGAGCATTGTTGTCTAAGTCGTATGTAGAAGCAATTGACATATTGGAAAGGATTTCTGCTAATAACTACCACTGGTCAGATTCCAGAGCTGATAGGAGCAATCATGGAGTTAATGATAATGAGGCAATGGCTGCACTTAATTTTCTATAG

Coding sequence (CDS)

ATGCATGATCTGCAAAGGCTAGAGTATCTACTTGACAATGAGATTGAATGTACATTTCCTAGGAGAATAAGGGAGCAACGTAGACAGCAAGTGGAAACTAACCCAATGGATCAATTACCAAGACTCCCTAATCCAATGGTTGAAGTAGTTGTACCAGCAGCTGCTCCCACTCTTAACACCATTCTATTGGCAGATGATGGAGAAAGGGCTATCAGAGCCTATGCTGCACTAGTACTTCATAGTTTTCATCCAGTTATAGCAGGTCCAGAGATAGAAGCTGAGAGGTTTGAATTAAAACCAGTTATGTTCCAGATGCTTCAAACAGTGGGGAAATTCTTTGGAAATCCATGTGAGGACTCCCATCTGCACTTGAGGTATTTTCTGGAAATAAAGAACTTCTACAATGGGTTGAGTGAAGCAACACGCCTGGTAGTTGATGCATCTGCTAATAGAGCATTGTTGTCTAAGTCGTATGTAGAAGCAATTGACATATTGGAAAGGATTTCTGCTAATAACTACCACTGGTCAGATTCCAGAGCTGATAGGAGCAATCATGGAGTTAATGATAATGAGGCAATGGCTGCACTTAATTTTCTATAG

Protein sequence

MHDLQRLEYLLDNEIECTFPRRIREQRRQQVETNPMDQLPRLPNPMVEVVVPAAAPTLNTILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDSHLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRADRSNHGVNDNEAMAALNFL
Homology
BLAST of Moc08g33220 vs. NCBI nr
Match: XP_022153147.1 (probable serine/threonine-protein kinase PBL11 [Momordica charantia])

HSP 1 Score: 382.5 bits (981), Expect = 2.2e-102
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0

Query: 1   MHDLQRLEYLLDNEIECTFPRRIREQRRQQVETNPMDQLPRLPNPMVEVVVPAAAPTLNT 60
           MHDLQRLEYLLDNEIECTFPRRIREQRRQQVETNPMDQLPRLPNPMVEVVVPAAAPTLNT
Sbjct: 1   MHDLQRLEYLLDNEIECTFPRRIREQRRQQVETNPMDQLPRLPNPMVEVVVPAAAPTLNT 60

Query: 61  ILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDS 120
           ILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDS
Sbjct: 61  ILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDS 120

Query: 121 HLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA 180
           HLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA
Sbjct: 121 HLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA 180

Query: 181 DRSNHGVNDNE 192
           DRSNHGVNDNE
Sbjct: 181 DRSNHGVNDNE 191

BLAST of Moc08g33220 vs. NCBI nr
Match: XP_022158490.1 (uncharacterized protein LOC111024970 [Momordica charantia])

HSP 1 Score: 174.9 bits (442), Expect = 7.1e-40
Identity = 103/186 (55.38%), Postives = 121/186 (65.05%), Query Frame = 0

Query: 36  MDQLPRLPNPMV------EVVVPAAAPTLNTILLADDGERAIRAYAALVLHSFHPVIAGP 95
           MDQLPRLPNP+V      EVVV AAAP LN ILL DDGER IRAYAA  +H FHPVIAGP
Sbjct: 1   MDQLPRLPNPVVEGINEGEVVVLAAAPPLNVILLVDDGERVIRAYAAPAIHGFHPVIAGP 60

Query: 96  EIEAERFELKPVMFQMLQTVGKFFGNPCEDSHLHLRYFLEIKNFYNG------------- 155
            IEAERFELK +MFQMLQTVG+FFGNP ED HLHLRYFLE+ + +N              
Sbjct: 61  AIEAERFELKSIMFQMLQTVGQFFGNPSEDPHLHLRYFLEVSDSFNMQEVSKEALRLKLF 120

Query: 156 ---LSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA--DRSNHGVNDNE 198
              LS+ TR  +++    ++ S + +      E  S      SDS+A  +R+NH  NDNE
Sbjct: 121 PYLLSDKTRDWLNSMPAESITSWNDLAEKFFNEIFSPKQECQSDSKAVNERNNHEANDNE 180

BLAST of Moc08g33220 vs. NCBI nr
Match: XP_015389226.1 (uncharacterized protein LOC107178482 [Citrus sinensis])

HSP 1 Score: 151.4 bits (381), Expect = 8.4e-33
Identity = 74/117 (63.25%), Postives = 89/117 (76.07%), Query Frame = 0

Query: 63  LADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDSHL 122
           +ADD +RAIR YA L     HP I  PE+EA  FELKP+MFQMLQT+G+F G P ED HL
Sbjct: 1   MADDRDRAIRDYAVLTPQVVHPGIVRPEVEAANFELKPMMFQMLQTLGQFNGLPTEDPHL 60

Query: 123 HLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSR 180
           HL+ FLE++ FYN L+ +TRL+VDASAN ALLSKSY EA +ILERI+ NNY W  +R
Sbjct: 61  HLKLFLELETFYNELNPSTRLMVDASANGALLSKSYTEAYEILERIANNNYQWPSTR 117

BLAST of Moc08g33220 vs. NCBI nr
Match: XP_030505151.1 (uncharacterized protein LOC115720131 [Cannabis sativa])

HSP 1 Score: 131.3 bits (329), Expect = 9.0e-27
Identity = 75/170 (44.12%), Postives = 101/170 (59.41%), Query Frame = 0

Query: 58  LNTILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPC 117
           +N I+LADD  RAIR Y A + +  +  I  P+I+A +FELKPVMFQM+QTVG F G P 
Sbjct: 15  VNPIILADDRARAIREYTAPMFNELNSGIVRPKIQAPQFELKPVMFQMIQTVGLFSGMPS 74

Query: 118 EDSHLHLRYFLEI-----------------------------KNFYNGLSEATRLVVDAS 177
           ED HLHL  FLE+                             + FYNGL+ A+++V+DAS
Sbjct: 75  EDPHLHLHSFLEVSDSFKLQRERFKELLQKCPHHGISHCIHMETFYNGLNVASQMVLDAS 134

Query: 178 ANRALLSKSYVEAIDILERISANNYHWSDSRADRSNH--GVNDNEAMAAL 197
            N A+ SKSY +A +ILE I++NNY WS++RA  S    GV + +A+  L
Sbjct: 135 TNGAIFSKSYNKAFEILETIASNNYQWSNTRAPTSRKVAGVVEVDAITTL 184

BLAST of Moc08g33220 vs. NCBI nr
Match: XP_015380966.1 (uncharacterized protein LOC107174510 [Citrus sinensis])

HSP 1 Score: 131.0 bits (328), Expect = 1.2e-26
Identity = 75/159 (47.17%), Postives = 97/159 (61.01%), Query Frame = 0

Query: 63  LADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDSHL 122
           +ADD +RAIR YA L     HP I  PE++A  FELKP+MFQMLQTVG+F G P ED HL
Sbjct: 1   MADDRDRAIRDYAVLTPQVVHPGIIRPEVDAANFELKPMMFQMLQTVGQFNGLPNEDPHL 60

Query: 123 HLRYFLEIKN----------------------FYNGLSEATRLVVDASANRALLSKSYVE 182
           H++ FLE++N                          L+ +TRL+VDA AN ALL KSY E
Sbjct: 61  HIKLFLELRNEITSFHQLEDESLYEVWERFKELLRRLNPSTRLMVDALANGALLFKSYTE 120

Query: 183 AIDILERISANNYHWSDSR--ADRSNHGVNDNEAMAALN 198
           A +ILERI+ NNY W  +R    R + GV++ +A+ AL+
Sbjct: 121 AYEILERIANNNYQWPSARQPTARGSVGVHNIDAITALS 159

BLAST of Moc08g33220 vs. ExPASy TrEMBL
Match: A0A6J1DI54 (probable serine/threonine-protein kinase PBL11 OS=Momordica charantia OX=3673 GN=LOC111020716 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.1e-102
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0

Query: 1   MHDLQRLEYLLDNEIECTFPRRIREQRRQQVETNPMDQLPRLPNPMVEVVVPAAAPTLNT 60
           MHDLQRLEYLLDNEIECTFPRRIREQRRQQVETNPMDQLPRLPNPMVEVVVPAAAPTLNT
Sbjct: 1   MHDLQRLEYLLDNEIECTFPRRIREQRRQQVETNPMDQLPRLPNPMVEVVVPAAAPTLNT 60

Query: 61  ILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDS 120
           ILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDS
Sbjct: 61  ILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCEDS 120

Query: 121 HLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA 180
           HLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA
Sbjct: 121 HLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA 180

Query: 181 DRSNHGVNDNE 192
           DRSNHGVNDNE
Sbjct: 181 DRSNHGVNDNE 191

BLAST of Moc08g33220 vs. ExPASy TrEMBL
Match: A0A6J1DVZ9 (uncharacterized protein LOC111024970 OS=Momordica charantia OX=3673 GN=LOC111024970 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 3.4e-40
Identity = 103/186 (55.38%), Postives = 121/186 (65.05%), Query Frame = 0

Query: 36  MDQLPRLPNPMV------EVVVPAAAPTLNTILLADDGERAIRAYAALVLHSFHPVIAGP 95
           MDQLPRLPNP+V      EVVV AAAP LN ILL DDGER IRAYAA  +H FHPVIAGP
Sbjct: 1   MDQLPRLPNPVVEGINEGEVVVLAAAPPLNVILLVDDGERVIRAYAAPAIHGFHPVIAGP 60

Query: 96  EIEAERFELKPVMFQMLQTVGKFFGNPCEDSHLHLRYFLEIKNFYNG------------- 155
            IEAERFELK +MFQMLQTVG+FFGNP ED HLHLRYFLE+ + +N              
Sbjct: 61  AIEAERFELKSIMFQMLQTVGQFFGNPSEDPHLHLRYFLEVSDSFNMQEVSKEALRLKLF 120

Query: 156 ---LSEATRLVVDASANRALLSKSYVEAIDILERISANNYHWSDSRA--DRSNHGVNDNE 198
              LS+ TR  +++    ++ S + +      E  S      SDS+A  +R+NH  NDNE
Sbjct: 121 PYLLSDKTRDWLNSMPAESITSWNDLAEKFFNEIFSPKQECQSDSKAVNERNNHEANDNE 180

BLAST of Moc08g33220 vs. ExPASy TrEMBL
Match: A0A6J1DX14 (uncharacterized protein LOC111025234 OS=Momordica charantia OX=3673 GN=LOC111025234 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 4.1e-25
Identity = 73/147 (49.66%), Postives = 93/147 (63.27%), Query Frame = 0

Query: 53  AAAPTLNTILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKF 112
           A  P LN ILL DD E+ IRAYAA     F+PVI  P IEA+RFELKP MFQMLQ    F
Sbjct: 27  ALTPPLNAILLGDDIEQEIRAYAAPAFFDFNPVIVDPIIEADRFELKPAMFQMLQI---F 86

Query: 113 FGNPCEDSHLHLRYFLEIKNFYNGLSEATRLVVDASANRALLSKSYVEAIDILERISANN 172
            G   ED + HL+YF+++ N     S+    V+DAS+N+ALL K Y EA DILE IS N 
Sbjct: 87  HGLASEDPYRHLQYFMQVAN----SSKVEEFVIDASSNKALLLKHYAEAFDILEEISRNK 146

Query: 173 YHWSDSRA--DRSNHGVNDNEAMAALN 198
           +  S SRA    ++ G+ +++ +A LN
Sbjct: 147 HQRSKSRAVSSTTSKGLANDDVVANLN 166

BLAST of Moc08g33220 vs. ExPASy TrEMBL
Match: A0A6J1EQ90 (uncharacterized protein LOC111436411 OS=Cucurbita moschata OX=3662 GN=LOC111436411 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.6e-24
Identity = 101/309 (32.69%), Postives = 133/309 (43.04%), Query Frame = 0

Query: 7   LEYLLDNEIECTFPRRIR------EQRRQQVETNPMDQLPR-LPNPMVEVVVPAAAPTLN 66
           LE++LD EIE TF RR++      EQ  QQ+E     QL R   NP   ++      T N
Sbjct: 7   LEFILDPEIERTFRRRLKKKKKMTEQNIQQIELGA--QLNREFENP--AMMANQERITAN 66

Query: 67  TILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFFGNPCED 126
            I LADD ERAIRAYA   +   +P I  PEI+   FELKPVMFQMLQT+G+F G P ED
Sbjct: 67  PIHLADDRERAIRAYAHPAVEELNPCIIRPEIQGTTFELKPVMFQMLQTIGQFHGLPLED 126

Query: 127 SHLHLRYFL--------------------------------------------------- 186
            HLHL+ FL                                                   
Sbjct: 127 PHLHLKSFLGVSDSFRFHSDSFRFQGVDKDMIRLSLFPYLLRDGAKSWLNTLAPGTIDSW 186

Query: 187 ----------------------------------------------------------EI 198
                                                                     ++
Sbjct: 187 NSLAENFLIKYFPPTRNARFKNEIVTFQQFEDETLSEACERFKEMLRKCPHHGLPHCIQM 246

BLAST of Moc08g33220 vs. ExPASy TrEMBL
Match: U5CUI2 (Retrotrans_gag domain-containing protein OS=Amborella trichopoda OX=13333 GN=AMTR_s04947p00003620 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 4.5e-24
Identity = 86/247 (34.82%), Postives = 109/247 (44.13%), Query Frame = 0

Query: 54  AAPTLNTILLADDGERAIRAYAALVLHSFHPVIAGPEIEAERFELKPVMFQMLQTVGKFF 113
           A   +N I+LADD  RAIR YAA + +  +P I  PEI+A +FELKPVMFQMLQTVG+F 
Sbjct: 6   AQQIVNPIILADDRARAIREYAAPMFNELNPGIVRPEIQAPQFELKPVMFQMLQTVGQFS 65

Query: 114 GNPCEDSHLHLRYFLEI------------------------------------------- 173
           G P ED HLHLR FLE+                                           
Sbjct: 66  GMPTEDPHLHLRSFLEVSDSFKIQGVSEEVLRLKLFPFSLRDRARSWLNTLPPDSVTNWN 125

Query: 174 -----------------------------------------------------------K 197
                                                                      +
Sbjct: 126 DLAEKFLRKYFPPTRNAKFRSEIMSFQQLEDESTSDAWERFKELLRKCPHHGIPHCIQME 185

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153147.12.2e-102100.00probable serine/threonine-protein kinase PBL11 [Momordica charantia][more]
XP_022158490.17.1e-4055.38uncharacterized protein LOC111024970 [Momordica charantia][more]
XP_015389226.18.4e-3363.25uncharacterized protein LOC107178482 [Citrus sinensis][more]
XP_030505151.19.0e-2744.12uncharacterized protein LOC115720131 [Cannabis sativa][more]
XP_015380966.11.2e-2647.17uncharacterized protein LOC107174510 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DI541.1e-102100.00probable serine/threonine-protein kinase PBL11 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1DVZ93.4e-4055.38uncharacterized protein LOC111024970 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1DX144.1e-2549.66uncharacterized protein LOC111025234 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1EQ902.6e-2432.69uncharacterized protein LOC111436411 OS=Cucurbita moschata OX=3662 GN=LOC1114364... [more]
U5CUI24.5e-2434.82Retrotrans_gag domain-containing protein OS=Amborella trichopoda OX=13333 GN=AMT... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g33220.1Moc08g33220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity