Moc08g31320 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g31320
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase H domain-containing protein
Location	chr8: 22446812 .. 22452030 (+)
RNA-Seq Expression	Moc08g31320
Synteny	Moc08g31320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGTCCCGAGCAATATCCTCTAACCATGTAAGTCAAAGTCTTGACACTGCCATAACAATCAACCAATACTATCAACTGTATACTCTGCTCTCTAGCTCAATAATTAGGTGCAGGTAATCCAGTATCTATTGCCTAGTGTAGCTTAAGTGGCTAACTAAAAAATAAGTAAATTATTAACATGTGTCTATTGGCCTCTAGGTTCTATCAATTTTTATTTTAGACTTTTTTTTTTGGATACGAAACAATTCATTGATGAAAAGAGAGGACAAACAGCCTACAGACAGGGGCCAAGGCAGCCCCTTCCAAAACACGAACTAATCACAAAAAGGCTCTCCAATCCTGTAAGATCAAAGAGAGGCTGTAATTACAAAAGAATTTCGTATTTCGTATTCACCACCAGGAAGCTAAGACCAACACACTATCACAAAAAGATTCCAAAGACTTATCTTTATCTTCAAAAGAGCGTGAATTCCTCCCCAGCCATAAATTCCACAAAAGAGCTCGAAAAACACATCTCCACAAGACTCTAGTTTTAAGTGTCACCCATGAAAGCATTCTAAAATCCAACTCTACAAGTCTTTTGGCATACAAATGTTAATATCAAAACACTGAAACAAGAACTACCATCCTTTAGTAGCAAGGGGACAGTGAATGAACAAGTGATCTAGAGTCTCTGCCCCTTTCCAACAGAGATAGCAAGCCGATGGGGACAGAGACCAATTCGGACACTTTGGAGCTTCTCCTGGGTGTTTAACGCACGATGAGCTAGGGACCAAAGAAAGACCTTCACCTTTTTTGGGATAGGGTCCTTCCTGTTATCAGAGAGGAATTTAATTTGGGAGGATTTTCCTTTAGCTTGAGGTATGCAGACTTTGTAGTGAAGTGTCCGTTTGGGTCCGAAGGCCACACTGCTACATCTGTTTCATCTCCTATCTCAAAAGCTTCCAAACGTTGAACCATTCTCATCCAGTTCTCAATTTCTCTGTCGAACAACCCTCTTCGGAAGCCTAGATCCCAAGTTCTCTGAACATTACACCAGCAATCTGCTATCACAGCCTCAGGTTTAAGAGAAATGGCAAACAGCCCTGGGAAATATTTTTATTTTAGACCTTCAGCTAGAAACTATTGATTTCTGTTTCTAAACTAGTTATTTGTTTGATTGTTTCCTTGTCTCGTTGTAACAATCAGAACCTGCCAGCACAGTAATATGCGGAACTATTGCATCTGTCAACTGAGATATCAAGTTAGATCATAATAAGTTTGTTAGCATGCATGGTAGTTTTGATACAAGTATATAATTTTAGAGTCGGCCCTAAGGGGTAGTCTAGTTGACAATAACTTGGGATCTCTTGGTCATACTGGCTTATAAGTCTCAAGTTCGAACCTTCCGATGAGTTAATACCAAAAATCCTTAATGTCTCTCGGGTTCGGGTCTTGGGGCGGGTGCGGGTGCCTCTGGGTATGAGGGAGCAAAGCTCCGACTCCCCAAGTTTTTGAACTCTCCCTTAATTTCCGTTCTTTGGAAATTCTCAATTCCAAAAAAGGCGAAAGTTTTCCTTTGGATCCTAGCCAACCGAGCTGTAAACACAGCAGATAGACTCCAGCGAAGATGTCCAAATTGGGCTCTTTCTCCTTCCATTTGTGTTCTTTGCAATCAAAATGAGGATACCATGGATCACATTTTTCTTCACTGCCAGTTTGCTCTTCGTGGGTGGTTCTTTCTGCTGCAAATCTTTGGGTACTCCATTTGTTTGTCTCAGAATATTGATGGATGGACGAATGAAGCTTTTCGGGGGTGTCATTTTAAAGGGAAAGCAAAAATTCTATGGAATTGTGCAGCTAGAGCCCTTATTTGGAACCTCTAGATGGAAAGAAACAATAGAATTTTTAAAGATAAGTCCTCTTTAGAAATTTTTTGTAACACTGTTCAATTACACACGTCTTGGTGGTGTTATAGGCATAGAAAATTCTTTTGTAATTATAGCCTCTCGTCAATTATGCAAGATTAGAGTGCTTTACTTCACTAGTTTTTGGGAGGGGGAGCCCTCTTCCCCCTGCCCTTAGGCTGTCTTTTGTCTCTATGCTTTTTATCCATATATAAAGTTTCTTATAAAGAAAAATAGTTACTTAATGATTATCATATTTTATTTGGGTACTAAAAAATGCGATGATGAAAATGAGTGGAATCTCTTTTTAGTTTTGTTGCTAAACTAACTGCTATTTTTACTTTCATCTCAGTGTCCAAATTCTTAAAAGATCTTTTTAAAATATTTCATGTACAGGAATGTTGCTTTCTAGAATTCGATGGTGCTTCGAAAGGAAATCCTGGGCAAGCTGGAGCAGGAGCTGTTCTGCGAGCACATGATGGAAGTGTGGTATACATTCTTCCTTTTGGAACCCACATTATTTATTTATTCTTTTTCATGTATGTCTAATCTCTCTCTCTCCCTTGGAGTAGATATGTAGACTGCGTGAAGGCTTAGGTACGGCAACCAATAATGTTGCTGAATATCGAGCTATTCTTTTAGGGTTGAAGTATGCTCTTCAGAAAGGTTTTACTAGGATCCATGTCCAAGGTGACTCCAAACTTGTCTGTATGCAGGTTGGTTAAAATCTTTTTCGCTAACAAACTTTGTTGTGTTATTTTTTGAACCTGGTCAACTCATTTCTTATTGTTTCTAAATCCATAAATCTGTTGGGTAGAATTATTTTTTGTCTTAATTTGTAGGGATGGGTTTAATGGTCGATCTTTGGATTTACCAAGAAAAACAAAGCTGAAAACTTCGTATTTAAAAGATTTCTTTTGTCAAATCTTGTGAAATTATTATTATTTTGTAGGGAACATTTGTGTTGAAGGTAAATAATGTTCCAAGTTGCATGAAAGCCAATGTCTTAATATAGGATGGGATGAACCAATAGCTCACCTTGCCCTGCATGCAATCTCTGCAATAATACTGATTCCATCTAGGATTTTACTACTGTTTCCTTCGGGGAGGGTAAAAAGAGACTCAACTTCTTTTTCTCAATGCACCTGCTCCGTAAAGATTCCCCCAATACCAAATTACGGAAGCGTTTGTTTAAAGAAAACCAACTTGATATTTTAAACACAAATATGGAGTGCCAAGTGGATGACAACCTCTTGGCATGGAGTTGAATCTGATATGATGCCACACCACTCATCATAACTAGCTCATTGCTTAAAATATTCTTTTCCGGTGGGGGGTGGAAGATCTTTCCTCGAGGTGTTAACAATTCCGGTCTTGGATTTGAAAAGTATAACGTCGTGAGTTCTTTTGAATTCCCAGTATTCTGTAATGTGGATTAGCATTTTGGAGGTTCTATAGAAAGTGTTGGGGAAGATCCAAATTTGAACCCAAACACAGTTTTTACATGTTCGATAATTACTAGGCATGCTCGTTTCAATTAAAGAACTAAAATGCATCCAGCTCCATCTTGTGGTTAGTTTTATTTTACAAGTTCTCATCTTCAGTTTCATTTTCTAGTAGCATGGTTTTGTTGAAATACTATTACAGTGTTTATTTGAATATTTTGTATGTGGGACTTCTAAACTCGGATTAAACCTGCTAGTATTCTGTAATTTTCTACCTCCTTATATCAAAACAACCTAATCTATCAATCCTAGCTCTCTGTGCAGTACCTGAATTCATCGTTTGTCCCCAACTTCTTAAATATTTTAAACCAAGCATATTTCATTTAACAGGTCCAAGGATTGTGGAAGGTAAAAAATGAGAACATCTCTGAGTTATGTAATGAAGTAATCAAGCTGAAGGATAAATTTCTCTCGTTCCAGATCAGTCATGTACTACGGGTCAGTGCATTAATTCTTTTCTATTTGCATATCTTACGGTGATGTTAGTTTGTTTCTGTATATTACTGTTTTTGTCCTCTTAAGCATTCAGTGTAGGCTCTTTGCTATTCGAGATCTGAAGGTTGGTAAAAGATATTTTGAACCTTAATCCCACTCGTTGATTTCTACAGTTAATTGAGCACTTCCGCGTTTGTTCATTACTACAGTTTTGATCTGATTCATACGTTTTTTTTTTTTTTTTTTTGCTTTTTCTAGTTTAGATTTCATTCATACTTAAGCGTGCGTTCTTTTTCCCATATAAATATAGGATTAAACTGTACGCTTTCGAGTTCAGTTTCTAGCAAACCAGTGACCTTATCAGTCAAGTTAGAGTTTGCACGTTGCTTTCTTCCCATCTGCCCTACCATATAAAAGACACTTATGTCTGCTTCTTAAACCTTAAGAATCACGGATACGGACACGCATGACACGGACATGACATGACACGGACACGGTGACACGTTATTTCCTAGAAAATTTGGGATGCGGATATGACAAAGACACGTTTGTTAAAAAATACAATTTTTAAGATGAAAATTCAAAGTCAATAAAATTATGCATTTTTATGCTTAAAAAAATGAATTTAATGTATGTTTGCTCTCAAAATTTATTGTTTTGTCCTATATTACTATTTATGTCTATTTCTTATGCTTAACAAGTGCGTGAAATGTACCTAACAAGTGTCCGGCCCATATTTATTTTGTACCACTAGTGTGTGACACACATGTATTGAGCTAACAAATGTCCGAAACATGAATCAGTGTCTTTGCTCAAATTGTAGTGTCTGTGCTTCTTAGCATCTTTAAACTGTCGATGATATAATCATTTCTATTGAAAATGAGGGAAAAAGTTCTTATCTGAGAAGAGGAATGAGGAAGAAGTGAATGAAACTAGATGCCAATTTCTTCAAGCAACTATTAGATCCCATTTTCTTAGCTGCTGATATTTACTCGTTCATCCTCTAAAATCTTAACATTCTGCTTTTCCATCCTTGAAAGAATCTTAATTCTGAAGCCGATGCTCAAGCAAACTTGGCTATCACTCTTGCTGGTAATGCTTCTCTTAAATAATTTTAGTAACCGTAAACTTTACGAGTTACTAAGGTGATCTTTCCTATTGCAGATGGCGAAGTCCTGGAGTATGAAGAATAGTTAGAATGCACGGCAGAATATATCTTACACATTAGCACATTTTTCTGACGAATGCATATTTATACGCCAATCCGTTGTATGCTACTATTCCTTTCCCAAGGTTGTCGAGATCTTTGGGTGTTGACCGTAACTCGTCCAACTGGAATTCAAGTTGTGACCTTGCTGCAATGCAGAAGTGCAGTTTACTGCATATCTGA

mRNA sequence

ATGGTGTCCCGAGCAATATCCTCTAACCATGAATGTTGCTTTCTAGAATTCGATGGTGCTTCGAAAGGAAATCCTGGGCAAGCTGGAGCAGGAGCTGTTCTGCGAGCACATGATGGAAGTGTGATATGTAGACTGCGTGAAGGCTTAGGTACGGCAACCAATAATGTTGCTGAATATCGAGCTATTCTTTTAGGGTTGAAGTATGCTCTTCAGAAAGGTTTTACTAGGATCCATGTCCAAGGTGACTCCAAACTTGTCTGTATGCAGGTCCAAGGATTGTGGAAGGTAAAAAATGAGAACATCTCTGAGTTATGTAATGAAGTAATCAAGCTGAAGGATAAATTTCTCTCGTTCCAGATCAGTCATGTACTACGGAATCTTAATTCTGAAGCCGATGCTCAAGCAAACTTGGCTATCACTCTTGCTGATGGCGAAGTCCTGGAGTTGTCGAGATCTTTGGGTGTTGACCGTAACTCGTCCAACTGGAATTCAAGTTGTGACCTTGCTGCAATGCAGAAGTGCAGTTTACTGCATATCTGA

Coding sequence (CDS)

ATGGTGTCCCGAGCAATATCCTCTAACCATGAATGTTGCTTTCTAGAATTCGATGGTGCTTCGAAAGGAAATCCTGGGCAAGCTGGAGCAGGAGCTGTTCTGCGAGCACATGATGGAAGTGTGATATGTAGACTGCGTGAAGGCTTAGGTACGGCAACCAATAATGTTGCTGAATATCGAGCTATTCTTTTAGGGTTGAAGTATGCTCTTCAGAAAGGTTTTACTAGGATCCATGTCCAAGGTGACTCCAAACTTGTCTGTATGCAGGTCCAAGGATTGTGGAAGGTAAAAAATGAGAACATCTCTGAGTTATGTAATGAAGTAATCAAGCTGAAGGATAAATTTCTCTCGTTCCAGATCAGTCATGTACTACGGAATCTTAATTCTGAAGCCGATGCTCAAGCAAACTTGGCTATCACTCTTGCTGATGGCGAAGTCCTGGAGTTGTCGAGATCTTTGGGTGTTGACCGTAACTCGTCCAACTGGAATTCAAGTTGTGACCTTGCTGCAATGCAGAAGTGCAGTTTACTGCATATCTGA

Protein sequence

MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYRAILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNLNSEADAQANLAITLADGEVLELSRSLGVDRNSSNWNSSCDLAAMQKCSLLHI

Homology

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BLAST of Moc08g31320 vs. NCBI nr
Match: XP_038889960.1 (uncharacterized protein LOC120079705 [Benincasa hispida])

HSP 1 Score: 277.7 bits (709), Expect = 7.0e-71
Identity = 139/148 (93.92%), Postives = 143/148 (96.62%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +VS A+SSN E CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYR
Sbjct: 220 IVSHALSSNRESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYR 279

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSF+I
Sbjct: 280 AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFEI 339

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLE 149
           +HVLRNLNSEADAQANLAITLADGEV E
Sbjct: 340 NHVLRNLNSEADAQANLAITLADGEVQE 367

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BLAST of Moc08g31320 vs. NCBI nr
Match: XP_022159181.1 (uncharacterized protein LOC111025600, partial [Momordica charantia])

HSP 1 Score: 274.6 bits (701), Expect = 5.9e-70
Identity = 137/138 (99.28%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           MVSRAISSN ECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR
Sbjct: 40  MVSRAISSNRECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 99

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI
Sbjct: 100 AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 159

Query: 121 SHVLRNLNSEADAQANLA 139
           SHVLRNLNSEADAQANLA
Sbjct: 160 SHVLRNLNSEADAQANLA 177

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BLAST of Moc08g31320 vs. NCBI nr
Match: XP_022999715.1 (uncharacterized protein LOC111493983 [Cucurbita maxima])

HSP 1 Score: 269.2 bits (687), Expect = 2.5e-68
Identity = 136/148 (91.89%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +VSRA  SNHE CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYR
Sbjct: 221 VVSRA-PSNHESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYR 280

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYAL+KGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEV+KLKDKFLSF+I
Sbjct: 281 AILLGLKYALEKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVMKLKDKFLSFEI 340

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLE 149
           SHVLRNLNSEADAQANLAI+L DGE  E
Sbjct: 341 SHVLRNLNSEADAQANLAISLPDGEAQE 367

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BLAST of Moc08g31320 vs. NCBI nr
Match: XP_022946833.1 (uncharacterized protein LOC111450776 [Cucurbita moschata] >KAG6599028.1 hypothetical protein SDJN03_08806, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 267.7 bits (683), Expect = 7.2e-68
Identity = 134/148 (90.54%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +VS+A  SNHE CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYR
Sbjct: 221 VVSQA-PSNHESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYR 280

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYAL+KGFTRIHVQGDSKLVCMQVQGLWKVKNENI+ELCNEV+KLKDKFLSF+I
Sbjct: 281 AILLGLKYALEKGFTRIHVQGDSKLVCMQVQGLWKVKNENIAELCNEVMKLKDKFLSFEI 340

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLE 149
           SHVLRNLNSEADAQANLA+TL DGE  E
Sbjct: 341 SHVLRNLNSEADAQANLAVTLPDGEAQE 367

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BLAST of Moc08g31320 vs. NCBI nr
Match: XP_023547239.1 (uncharacterized protein LOC111806115 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 266.5 bits (680), Expect = 1.6e-67
Identity = 133/148 (89.86%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +VS+A  SNHE CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYR
Sbjct: 221 VVSQA-PSNHESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYR 280

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYAL+KGFTRIHVQGDSKLVCMQVQGLWKVKNENI+ELCNEV+KLKDKFLSF+I
Sbjct: 281 AILLGLKYALEKGFTRIHVQGDSKLVCMQVQGLWKVKNENIAELCNEVMKLKDKFLSFEI 340

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLE 149
           SHVLRNLNSEADA+ANLA+TL DGE  E
Sbjct: 341 SHVLRNLNSEADAEANLAVTLPDGEAQE 367

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BLAST of Moc08g31320 vs. ExPASy Swiss-Prot
Match: P9WLH5 (Bifunctional protein Rv2228c OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2228c PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-17
Identity = 55/129 (42.64%), Postives = 75/129 (58.14%), Query Frame = 0


Query: 15  LEFDGASKGNPGQAGAGAVLRAHDGS-VICRLREGLGTATNNVAEYRAILLGLKYALQKG 74
           +E DG S+GNPG AG GAV+   D S V+   ++ +G ATNNVAEYR ++ GL  A++ G
Sbjct: 5   IEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVKLG 64

Query: 75  FTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNLNSEADA 134
            T   V  DSKLV  Q+ G WKVK+ ++ +L  +   L  +F       V R  N+ AD 
Sbjct: 65  ATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALASQFRRINYEWVPRARNTYADR 124

Query: 135 QANLAITLA 143
            AN A+  A
Sbjct: 125 LANDAMDAA 133

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BLAST of Moc08g31320 vs. ExPASy Swiss-Prot
Match: P9WLH4 (Uncharacterized protein MT2287 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2287 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-17
Identity = 55/129 (42.64%), Postives = 75/129 (58.14%), Query Frame = 0


Query: 15  LEFDGASKGNPGQAGAGAVLRAHDGS-VICRLREGLGTATNNVAEYRAILLGLKYALQKG 74
           +E DG S+GNPG AG GAV+   D S V+   ++ +G ATNNVAEYR ++ GL  A++ G
Sbjct: 5   IEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVKLG 64

Query: 75  FTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNLNSEADA 134
            T   V  DSKLV  Q+ G WKVK+ ++ +L  +   L  +F       V R  N+ AD 
Sbjct: 65  ATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALASQFRRINYEWVPRARNTYADR 124

Query: 135 QANLAITLA 143
            AN A+  A
Sbjct: 125 LANDAMDAA 133

[Close]

BLAST of Moc08g31320 vs. ExPASy Swiss-Prot
Match: P64956 (Uncharacterized protein Mb2253c OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2253C PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-17
Identity = 55/129 (42.64%), Postives = 75/129 (58.14%), Query Frame = 0


Query: 15  LEFDGASKGNPGQAGAGAVLRAHDGS-VICRLREGLGTATNNVAEYRAILLGLKYALQKG 74
           +E DG S+GNPG AG GAV+   D S V+   ++ +G ATNNVAEYR ++ GL  A++ G
Sbjct: 5   IEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVKLG 64

Query: 75  FTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNLNSEADA 134
            T   V  DSKLV  Q+ G WKVK+ ++ +L  +   L  +F       V R  N+ AD 
Sbjct: 65  ATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALASQFRRINYEWVPRARNTYADR 124

Query: 135 QANLAITLA 143
            AN A+  A
Sbjct: 125 LANDAMDAA 133

[Close]

BLAST of Moc08g31320 vs. ExPASy Swiss-Prot
Match: Q9HSF6 (Ribonuclease HI OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OX=64091 GN=rnhA PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.6e-17
Identity = 50/123 (40.65%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 17  FDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYRAILLGLKYALQKGFTR 76
           FDGAS+GNPG A  G VL + DG ++    + +G ATNN AEY A++  L+ A   GF  
Sbjct: 74  FDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDD 133

Query: 77  IHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNLNSEADAQAN 136
           I ++GDS+LV  Q+ G W   + ++        +L   F  + I+HV R  N  ADA AN
Sbjct: 134 IELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPRATNERADALAN 193

Query: 137 LAI 140
            A+
Sbjct: 194 EAL 196

[Close]

BLAST of Moc08g31320 vs. ExPASy Swiss-Prot
Match: F9VN79 (Ribonuclease HI OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=rnhA PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 34/90 (37.78%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 52  ATNNVAEYRAILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKL 111
           +TNNVAEY  ++  ++  L+ G +   ++GDS+LV  Q+ G +KVK + I  L  + I+L
Sbjct: 46  STNNVAEYSGLICLMETMLRLGISSPIIKGDSQLVIKQMNGEYKVKAKRIIPLYEKAIEL 105

Query: 112 KDKFLSFQISHVLRNLNSEADAQANLAITL 142
           K K L+  +  V R  N EAD  + +A  L
Sbjct: 106 KKK-LNATLIWVPREENKEADRLSRVAYEL 134

[Close]

BLAST of Moc08g31320 vs. ExPASy TrEMBL
Match: A0A6J1E352 (uncharacterized protein LOC111025600 OS=Momordica charantia OX=3673 GN=LOC111025600 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.9e-70
Identity = 137/138 (99.28%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           MVSRAISSN ECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR
Sbjct: 40  MVSRAISSNRECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 99

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI
Sbjct: 100 AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 159

Query: 121 SHVLRNLNSEADAQANLA 139
           SHVLRNLNSEADAQANLA
Sbjct: 160 SHVLRNLNSEADAQANLA 177

[Close]

BLAST of Moc08g31320 vs. ExPASy TrEMBL
Match: A0A6J1KGD7 (uncharacterized protein LOC111493983 OS=Cucurbita maxima OX=3661 GN=LOC111493983 PE=4 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.2e-68
Identity = 136/148 (91.89%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +VSRA  SNHE CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYR
Sbjct: 221 VVSRA-PSNHESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYR 280

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYAL+KGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEV+KLKDKFLSF+I
Sbjct: 281 AILLGLKYALEKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVMKLKDKFLSFEI 340

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLE 149
           SHVLRNLNSEADAQANLAI+L DGE  E
Sbjct: 341 SHVLRNLNSEADAQANLAISLPDGEAQE 367

[Close]

BLAST of Moc08g31320 vs. ExPASy TrEMBL
Match: A0A6J1G4Y7 (uncharacterized protein LOC111450776 OS=Cucurbita moschata OX=3662 GN=LOC111450776 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 3.5e-68
Identity = 134/148 (90.54%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +VS+A  SNHE CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYR
Sbjct: 221 VVSQA-PSNHESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYR 280

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           AILLGLKYAL+KGFTRIHVQGDSKLVCMQVQGLWKVKNENI+ELCNEV+KLKDKFLSF+I
Sbjct: 281 AILLGLKYALEKGFTRIHVQGDSKLVCMQVQGLWKVKNENIAELCNEVMKLKDKFLSFEI 340

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLE 149
           SHVLRNLNSEADAQANLA+TL DGE  E
Sbjct: 341 SHVLRNLNSEADAQANLAVTLPDGEAQE 367

[Close]

BLAST of Moc08g31320 vs. ExPASy TrEMBL
Match: A0A5A7TCE2 (RNase H family protein, putative isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00790 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.3e-67
Identity = 128/144 (88.89%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 5   AISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYRAILL 64
           ++SSNHE CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYRAILL
Sbjct: 230 SLSSNHESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYRAILL 289

Query: 65  GLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVL 124
           GLK+AL+KGFTRIHVQGDSKLVCMQVQGLWK KNENISELCNEV+KLK+KFLSF+++HVL
Sbjct: 290 GLKFALKKGFTRIHVQGDSKLVCMQVQGLWKAKNENISELCNEVVKLKNKFLSFEVNHVL 349

Query: 125 RNLNSEADAQANLAITLADGEVLE 149
           R+LNSEADAQANLA+TLADGE+ E
Sbjct: 350 RHLNSEADAQANLALTLADGEIQE 373

[Close]

BLAST of Moc08g31320 vs. ExPASy TrEMBL
Match: A0A1S3CPT7 (uncharacterized protein LOC103503315 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503315 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.3e-67
Identity = 128/144 (88.89%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 5   AISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYRAILL 64
           ++SSNHE CFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLG ATNNVAEYRAILL
Sbjct: 230 SLSSNHESCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGIATNNVAEYRAILL 289

Query: 65  GLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVL 124
           GLK+AL+KGFTRIHVQGDSKLVCMQVQGLWK KNENISELCNEV+KLK+KFLSF+++HVL
Sbjct: 290 GLKFALKKGFTRIHVQGDSKLVCMQVQGLWKAKNENISELCNEVVKLKNKFLSFEVNHVL 349

Query: 125 RNLNSEADAQANLAITLADGEVLE 149
           R+LNSEADAQANLA+TLADGE+ E
Sbjct: 350 RHLNSEADAQANLALTLADGEIQE 373

[Close]

BLAST of Moc08g31320 vs. TAIR 10
Match: AT1G24090.1 (RNase H family protein )

HSP 1 Score: 184.5 bits (467), Expect = 7.5e-47
Identity = 87/136 (63.97%), Postives = 112/136 (82.35%), Query Frame = 0


Query: 11  ECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYRAILLGLKYAL 70
           E CF+EFDGASKGNPG +GA AVL+  DGS+ICR+R+GLG ATNN AEY A++LGLKYA+
Sbjct: 215 ETCFIEFDGASKGNPGLSGAAAVLKTEDGSLICRVRQGLGIATNNAAEYHALILGLKYAI 274

Query: 71  QKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNLNSE 130
           +KG+  I V+GDSKLVCMQ++G WKV +E +++L  E   L +K +SF+ISHVLRNLN++
Sbjct: 275 EKGYKNIKVKGDSKLVCMQIKGQWKVNHEVLAKLHKEAKLLCNKCVSFEISHVLRNLNAD 334

Query: 131 ADAQANLAITLADGEV 147
           AD QANLA+ L +GEV
Sbjct: 335 ADEQANLAVRLPEGEV 350

[Close]

BLAST of Moc08g31320 vs. TAIR 10
Match: AT3G01410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 3.1e-45
Identity = 88/150 (58.67%), Postives = 110/150 (73.33%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +++R     ++ C +EFDGASKGNPG+AGAGAVLRA D SV+  LREG+G ATNNVAEYR
Sbjct: 143 LLTRTPIRQNDSCTIEFDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYR 202

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           A+LLGL+ AL KGF  +HV GDS LVCMQVQG WK  +  ++ELC +  +L + F +F I
Sbjct: 203 ALLLGLRSALDKGFKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDI 262

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLELS 151
            H+ R  NSEAD QAN AI LADG+   +S
Sbjct: 263 KHIAREKNSEADKQANSAIFLADGQTQVIS 292

[Close]

BLAST of Moc08g31320 vs. TAIR 10
Match: AT3G01410.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 3.1e-45
Identity = 88/150 (58.67%), Postives = 110/150 (73.33%), Query Frame = 0


Query: 1   MVSRAISSNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYR 60
           +++R     ++ C +EFDGASKGNPG+AGAGAVLRA D SV+  LREG+G ATNNVAEYR
Sbjct: 143 LLTRTPIRQNDSCTIEFDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYR 202

Query: 61  AILLGLKYALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQI 120
           A+LLGL+ AL KGF  +HV GDS LVCMQVQG WK  +  ++ELC +  +L + F +F I
Sbjct: 203 ALLLGLRSALDKGFKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDI 262

Query: 121 SHVLRNLNSEADAQANLAITLADGEVLELS 151
            H+ R  NSEAD QAN AI LADG+   +S
Sbjct: 263 KHIAREKNSEADKQANSAIFLADGQTQVIS 292

[Close]

BLAST of Moc08g31320 vs. TAIR 10
Match: AT5G51080.1 (RNase H family protein )

HSP 1 Score: 178.3 bits (451), Expect = 5.4e-45
Identity = 84/139 (60.43%), Postives = 114/139 (82.01%), Query Frame = 0


Query: 8   SNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYRAILLGLK 67
           +++E C +EFDGASKGNPG +GA AVL+  DGS+I ++R+GLG ATNN AEY  ++LGLK
Sbjct: 181 TSYETCIIEFDGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLK 240

Query: 68  YALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNL 127
           +A++KG+T+I V+ DSKLVCMQ++G WKV +E +S+L  E  +L DK LSF+ISHVLR+L
Sbjct: 241 HAIEKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLRSL 300

Query: 128 NSEADAQANLAITLADGEV 147
           NS+AD QAN+A  L++GEV
Sbjct: 301 NSDADEQANMAARLSEGEV 319

[Close]

BLAST of Moc08g31320 vs. TAIR 10
Match: AT5G51080.2 (RNase H family protein )

HSP 1 Score: 178.3 bits (451), Expect = 5.4e-45
Identity = 84/139 (60.43%), Postives = 114/139 (82.01%), Query Frame = 0


Query: 8   SNHECCFLEFDGASKGNPGQAGAGAVLRAHDGSVICRLREGLGTATNNVAEYRAILLGLK 67
           +++E C +EFDGASKGNPG +GA AVL+  DGS+I ++R+GLG ATNN AEY  ++LGLK
Sbjct: 181 TSYETCIIEFDGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLK 240

Query: 68  YALQKGFTRIHVQGDSKLVCMQVQGLWKVKNENISELCNEVIKLKDKFLSFQISHVLRNL 127
           +A++KG+T+I V+ DSKLVCMQ++G WKV +E +S+L  E  +L DK LSF+ISHVLR+L
Sbjct: 241 HAIEKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLRSL 300

Query: 128 NSEADAQANLAITLADGEV 147
           NS+AD QAN+A  L++GEV
Sbjct: 301 NSDADEQANMAARLSEGEV 319

The following BLAST results are available for this feature:

BLAST of Moc08g31320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889960.1	7.0e-71	93.92	uncharacterized protein LOC120079705 [Benincasa hispida]	[more]
XP_022159181.1	5.9e-70	99.28	uncharacterized protein LOC111025600, partial [Momordica charantia]	[more]
XP_022999715.1	2.5e-68	91.89	uncharacterized protein LOC111493983 [Cucurbita maxima]	[more]
XP_022946833.1	7.2e-68	90.54	uncharacterized protein LOC111450776 [Cucurbita moschata] >KAG6599028.1 hypothet...	[more]
XP_023547239.1	1.6e-67	89.86	uncharacterized protein LOC111806115 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc08g31320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P9WLH5	1.2e-17	42.64	Bifunctional protein Rv2228c OS=Mycobacterium tuberculosis (strain ATCC 25618 / ...	[more]
P9WLH4	1.2e-17	42.64	Uncharacterized protein MT2287 OS=Mycobacterium tuberculosis (strain CDC 1551 / ...	[more]
P64956	1.2e-17	42.64	Uncharacterized protein Mb2253c OS=Mycobacterium bovis (strain ATCC BAA-935 / AF...	[more]
Q9HSF6	4.6e-17	40.65	Ribonuclease HI OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC...	[more]
F9VN79	1.6e-06	37.78	Ribonuclease HI OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC ...	[more]

BLAST of Moc08g31320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E352	2.9e-70	99.28	uncharacterized protein LOC111025600 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1KGD7	1.2e-68	91.89	uncharacterized protein LOC111493983 OS=Cucurbita maxima OX=3661 GN=LOC111493983...	[more]
A0A6J1G4Y7	3.5e-68	90.54	uncharacterized protein LOC111450776 OS=Cucurbita moschata OX=3662 GN=LOC1114507...	[more]
A0A5A7TCE2	1.3e-67	88.89	RNase H family protein, putative isoform 2 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3CPT7	1.3e-67	88.89	uncharacterized protein LOC103503315 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Moc08g31320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24090.1	7.5e-47	63.97	RNase H family protein	[more]
AT3G01410.1	3.1e-45	58.67	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G01410.2	3.1e-45	58.67	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G51080.1	5.4e-45	60.43	RNase H family protein	[more]
AT5G51080.2	5.4e-45	60.43	RNase H family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 17..138 e-value: 6.3E-31 score: 106.8
IPR002156	Ribonuclease H domain	PROSITE	PS50879	RNASE_H	coord: 9..140 score: 15.947225
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 13..143 e-value: 6.0E-38 score: 131.9
None	No IPR available	PANTHER	PTHR46387	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN	coord: 9..148
None	No IPR available	PANTHER	PTHR46387:SF14	PUTATIVE-RELATED	coord: 9..148
None	No IPR available	CDD	cd09279	RNase_HI_like	coord: 13..139 e-value: 1.56409E-51 score: 159.56
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 12..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g31320.1	Moc08g31320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity