Moc08g30000 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g30000
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionmetalloendoproteinase 2-MMP
Locationchr8: 21480013 .. 21481140 (-)
RNA-Seq ExpressionMoc08g30000
SyntenyMoc08g30000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCCCCAAATTTCATTGTCCTCCTCCTTATTTCCCTCTCCCTCTTCTCCCCTCCAATAATCAACGCCCACTTGTTCCCCAACGCCTCCGCCATCCCCTCGTGGCTCAACAACACCAAATGCCTTTGGGACCAATTCCAGAAGCTCAAAGGATGCCGCCCCGGCGACCGCCTCCAAGGCCTCTCCTCTCTCAAGACTTACTTCAAACACTTCGGCTACATCCCTAATCCCAATCCCAACTCCAATTTCACCGATCTCTTCGACGATATTCTCCACTCCGCCCTCACCACCTACCAGAAGAATTTCAATCTCAACGTCTCCGGCGAGCTCGATGACGACACTCTCCGCCAGATCCAGCTCCCCAGATGCGGCGTCGCCGATATAATCAATGGCTCCAGTTCTATGAACTCCGGGAACCCCGCTGCCCATGCCGCTTCCGGTTCTGCTCGCTTCCACTCTGTTTCTCACTACTCTTTCTTCCCAGGGAAGCCGCGCTGGCCGGCGGGTCGCCGGGATCTGACTTATGCCTTCGCGCCGGAGAATCAGCTCAGCGCTGAGGTGAAGGCTGTGTTCGGCCGGGCGTTCGGGCGGTGGGCGGCGGTGACGCCGTTGACTTTCACGGAGGTTCAGTCGTTCAGGGCGGCGGATATTAGGATTGGGTTCTTCGCTGGAGATCACGGCGATGGGGAGCCGTTCGACGGCGTTTTGGGGACTCTGGCGCACGCGTTTTCGCCGCCGAGTGGGCACTTCCATCTGGACGGCGACGAGAGCTGGGTGGTCTCCGGGGACCTGGGGACGGCTCCGGCGGCGGCGATTGACCTGGAGTCGGTGGCGGTGCACGAGATTGGGCATTTGTTGGGGCTGGGCCACTCCTCCGTCGAGGAATCGATTATGTTCCCGACGATCACGTCGCGGACGAGGAAGGTGGATCTCGCCGCCGACGACGTTGCCGGAGTGCAGGAGCTATACGGCGGCAATCCTAACGGCGACAACTCGACGACGACGACGATGCCGCCGTCGTCCGTACAGGAGAGGGACACGTCCAAAAACGGGGCTGCCACGCTGCTCAGCCTCTGGTGGGGCCCACTTTTACTTACTGCCGTTGAACTTTTCCTGATGAGATAA

mRNA sequence

ATGAAATCCCCAAATTTCATTGTCCTCCTCCTTATTTCCCTCTCCCTCTTCTCCCCTCCAATAATCAACGCCCACTTGTTCCCCAACGCCTCCGCCATCCCCTCGTGGCTCAACAACACCAAATGCCTTTGGGACCAATTCCAGAAGCTCAAAGGATGCCGCCCCGGCGACCGCCTCCAAGGCCTCTCCTCTCTCAAGACTTACTTCAAACACTTCGGCTACATCCCTAATCCCAATCCCAACTCCAATTTCACCGATCTCTTCGACGATATTCTCCACTCCGCCCTCACCACCTACCAGAAGAATTTCAATCTCAACGTCTCCGGCGAGCTCGATGACGACACTCTCCGCCAGATCCAGCTCCCCAGATGCGGCGTCGCCGATATAATCAATGGCTCCAGTTCTATGAACTCCGGGAACCCCGCTGCCCATGCCGCTTCCGGTTCTGCTCGCTTCCACTCTGTTTCTCACTACTCTTTCTTCCCAGGGAAGCCGCGCTGGCCGGCGGGTCGCCGGGATCTGACTTATGCCTTCGCGCCGGAGAATCAGCTCAGCGCTGAGGTGAAGGCTGTGTTCGGCCGGGCGTTCGGGCGGTGGGCGGCGGTGACGCCGTTGACTTTCACGGAGGTTCAGTCGTTCAGGGCGGCGGATATTAGGATTGGGTTCTTCGCTGGAGATCACGGCGATGGGGAGCCGTTCGACGGCGTTTTGGGGACTCTGGCGCACGCGTTTTCGCCGCCGAGTGGGCACTTCCATCTGGACGGCGACGAGAGCTGGGTGGTCTCCGGGGACCTGGGGACGGCTCCGGCGGCGGCGATTGACCTGGAGTCGGTGGCGGTGCACGAGATTGGGCATTTGTTGGGGCTGGGCCACTCCTCCGTCGAGGAATCGATTATGTTCCCGACGATCACGTCGCGGACGAGGAAGGTGGATCTCGCCGCCGACGACGTTGCCGGAGTGCAGGAGCTATACGGCGGCAATCCTAACGGCGACAACTCGACGACGACGACGATGCCGCCGTCGTCCGTACAGGAGAGGGACACGTCCAAAAACGGGGCTGCCACGCTGCTCAGCCTCTGGTGGGGCCCACTTTTACTTACTGCCGTTGAACTTTTCCTGATGAGATAA

Coding sequence (CDS)

ATGAAATCCCCAAATTTCATTGTCCTCCTCCTTATTTCCCTCTCCCTCTTCTCCCCTCCAATAATCAACGCCCACTTGTTCCCCAACGCCTCCGCCATCCCCTCGTGGCTCAACAACACCAAATGCCTTTGGGACCAATTCCAGAAGCTCAAAGGATGCCGCCCCGGCGACCGCCTCCAAGGCCTCTCCTCTCTCAAGACTTACTTCAAACACTTCGGCTACATCCCTAATCCCAATCCCAACTCCAATTTCACCGATCTCTTCGACGATATTCTCCACTCCGCCCTCACCACCTACCAGAAGAATTTCAATCTCAACGTCTCCGGCGAGCTCGATGACGACACTCTCCGCCAGATCCAGCTCCCCAGATGCGGCGTCGCCGATATAATCAATGGCTCCAGTTCTATGAACTCCGGGAACCCCGCTGCCCATGCCGCTTCCGGTTCTGCTCGCTTCCACTCTGTTTCTCACTACTCTTTCTTCCCAGGGAAGCCGCGCTGGCCGGCGGGTCGCCGGGATCTGACTTATGCCTTCGCGCCGGAGAATCAGCTCAGCGCTGAGGTGAAGGCTGTGTTCGGCCGGGCGTTCGGGCGGTGGGCGGCGGTGACGCCGTTGACTTTCACGGAGGTTCAGTCGTTCAGGGCGGCGGATATTAGGATTGGGTTCTTCGCTGGAGATCACGGCGATGGGGAGCCGTTCGACGGCGTTTTGGGGACTCTGGCGCACGCGTTTTCGCCGCCGAGTGGGCACTTCCATCTGGACGGCGACGAGAGCTGGGTGGTCTCCGGGGACCTGGGGACGGCTCCGGCGGCGGCGATTGACCTGGAGTCGGTGGCGGTGCACGAGATTGGGCATTTGTTGGGGCTGGGCCACTCCTCCGTCGAGGAATCGATTATGTTCCCGACGATCACGTCGCGGACGAGGAAGGTGGATCTCGCCGCCGACGACGTTGCCGGAGTGCAGGAGCTATACGGCGGCAATCCTAACGGCGACAACTCGACGACGACGACGATGCCGCCGTCGTCCGTACAGGAGAGGGACACGTCCAAAAACGGGGCTGCCACGCTGCTCAGCCTCTGGTGGGGCCCACTTTTACTTACTGCCGTTGAACTTTTCCTGATGAGATAA

Protein sequence

MKSPNFIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATLLSLWWGPLLLTAVELFLMR
Homology
BLAST of Moc08g30000 vs. NCBI nr
Match: XP_022999578.1 (metalloendoproteinase 2-MMP [Cucurbita maxima])

HSP 1 Score: 571.6 bits (1472), Expect = 4.9e-159
Identity = 286/372 (76.88%), Postives = 316/372 (84.95%), Query Frame = 0

Query: 7   IVLLLISLSLFS-PPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 66
           ++L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCR GD L GLS+L
Sbjct: 6   MILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRHGDHLTGLSNL 65

Query: 67  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 126
           KTY   FGYI  PN  SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG
Sbjct: 66  KTYLNKFGYI--PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCG 125

Query: 127 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 186
           +ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+
Sbjct: 126 IADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 185

Query: 187 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 246
            EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 186 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 245

Query: 247 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 306
           PPSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 246 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305

Query: 307 RTRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTSKNGAATLLSLWWGP 366
           RTRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD SK GA  +L+ WW P
Sbjct: 306 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSKGGAPKVLNSWWSP 365

Query: 367 LLLTAVELFLMR 376
           L++ AVELFLMR
Sbjct: 366 LVVAAVELFLMR 372

BLAST of Moc08g30000 vs. NCBI nr
Match: XP_022946065.1 (metalloendoproteinase 5-MMP-like [Cucurbita moschata])

HSP 1 Score: 571.2 bits (1471), Expect = 6.4e-159
Identity = 287/373 (76.94%), Postives = 317/373 (84.99%), Query Frame = 0

Query: 7   IVLLLISLSLFS-PPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 66
           ++L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+L
Sbjct: 6   LILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLNGLSNL 65

Query: 67  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 126
           KTY   FGYI  PN  SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG
Sbjct: 66  KTYLNKFGYI--PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCG 125

Query: 127 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 186
           +ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+
Sbjct: 126 IADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 185

Query: 187 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 246
            EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 186 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 245

Query: 247 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 306
           PPSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 246 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305

Query: 307 RTRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWG 366
           RTRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW 
Sbjct: 306 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 365

Query: 367 PLLLTAVELFLMR 376
           PL++ AVELFLMR
Sbjct: 366 PLVVAAVELFLMR 373

BLAST of Moc08g30000 vs. NCBI nr
Match: KAG7029995.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.9 bits (1470), Expect = 8.4e-159
Identity = 287/373 (76.94%), Postives = 317/373 (84.99%), Query Frame = 0

Query: 7   IVLLLISLSLFS-PPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 66
           ++L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+L
Sbjct: 6   LILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNL 65

Query: 67  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 126
           KTY   FGYI  PN  SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG
Sbjct: 66  KTYLNKFGYI--PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCG 125

Query: 127 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 186
           +ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+
Sbjct: 126 IADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 185

Query: 187 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 246
            EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 186 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 245

Query: 247 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 306
           PPSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 246 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305

Query: 307 RTRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWG 366
           RTRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW 
Sbjct: 306 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 365

Query: 367 PLLLTAVELFLMR 376
           PL++ AVELFLMR
Sbjct: 366 PLVVAAVELFLMR 373

BLAST of Moc08g30000 vs. NCBI nr
Match: XP_023546725.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 568.5 bits (1464), Expect = 4.1e-158
Identity = 286/373 (76.68%), Postives = 316/373 (84.72%), Query Frame = 0

Query: 7   IVLLLISLSLFS-PPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 66
           ++L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+L
Sbjct: 6   LILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLTGLSNL 65

Query: 67  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 126
           KTY   FGYI  PN  SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG
Sbjct: 66  KTYLNKFGYI--PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCG 125

Query: 127 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 186
           +ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+
Sbjct: 126 IADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 185

Query: 187 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 246
            EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 186 NEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 245

Query: 247 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 306
           PPSGHFHLD DESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 246 PPSGHFHLDSDESWVVNGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305

Query: 307 RTRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWG 366
           RTRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW 
Sbjct: 306 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 365

Query: 367 PLLLTAVELFLMR 376
           PL++ AVELFLMR
Sbjct: 366 PLVVAAVELFLMR 373

BLAST of Moc08g30000 vs. NCBI nr
Match: XP_038889498.1 (metalloendoproteinase 2-MMP [Benincasa hispida])

HSP 1 Score: 565.8 bits (1457), Expect = 2.7e-157
Identity = 281/369 (76.15%), Postives = 311/369 (84.28%), Query Frame = 0

Query: 8   VLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSLKT 67
           +L+ +SL L + P I+AH+FPN S+IPSWLN T C W+QFQKLKGCRPGDRL GLS+LKT
Sbjct: 4   LLISLSLLLLAYP-ISAHIFPNVSSIPSWLNKTNCAWNQFQKLKGCRPGDRLNGLSNLKT 63

Query: 68  YFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGVA 127
           Y K FGYIP   P+ NFTDLFD +L SAL TYQ NFNLNV+G LDD TL QIQLPRCG+ 
Sbjct: 64  YLKQFGYIP---PSPNFTDLFDPLLQSALQTYQTNFNLNVTGLLDDQTLAQIQLPRCGIP 123

Query: 128 DIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSAE 187
           DIIN SSSMNSG P  H+A+    FHSVSHYSFFPG+P WP+ RR LTYAFAPEN LS E
Sbjct: 124 DIINNSSSMNSGKPTGHSAT---HFHSVSHYSFFPGRPTWPSHRRHLTYAFAPENPLSDE 183

Query: 188 VKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSPP 247
           VK VF RAF RWAAVTPLTF+ V+SFR ADIRIGF+AGDHGDGEPFDGVLGTLAHAFSPP
Sbjct: 184 VKGVFARAFARWAAVTPLTFSPVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFSPP 243

Query: 248 SGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT 307
           SGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT
Sbjct: 244 SGHFHLDGDESWVVSGDLRSAPMAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRT 303

Query: 308 RKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAA-TLLSLWWGPLLL 367
           RKVDLAADD+ G+QELYGGNP+G+ +  +T  P SVQERD S+NGAA  LLS WW PLL+
Sbjct: 304 RKVDLAADDINGIQELYGGNPDGNLTPPSTPSPPSVQERDMSRNGAAPRLLSFWWSPLLI 363

Query: 368 TAVELFLMR 376
            AVE FL+R
Sbjct: 364 AAVEFFLIR 365

BLAST of Moc08g30000 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 4.0e-111
Identity = 202/354 (57.06%), Postives = 251/354 (70.90%), Query Frame = 0

Query: 7   IVLLLISLSLFSPPIINAHLFPNASAIPSWL--NNTKCLWDQFQKLKGCRPGDRLQGLSS 66
           I + ++ L  F+P  ++A  + N+SAIP  L  N T   W+ F    GC  G +  GL  
Sbjct: 4   ICVFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGLYM 63

Query: 67  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 126
           LK YF+HFGYI   N + NFTD FDDIL +A+  YQ+NF LNV+G LD+ TL+ + +PRC
Sbjct: 64  LKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRC 123

Query: 127 GVADIINGSSSMNSGNPAAHA--ASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPEN 186
           G  D++NG+S+M+SG        A    RFH+V HYSFFPG+PRWP  RRDLTYAF P N
Sbjct: 124 GNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRN 183

Query: 187 QLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAH 246
            L+ EVK+VF RAF RW  VTPLTFT V+ F  +DI IGF++G+HGDGEPFDG + TLAH
Sbjct: 184 ALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAH 243

Query: 247 AFSPPSGHFHLDGDESWVVSGDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIM 306
           AFSPP+GHFHLDG+E+W+VSG+ G    + + A+DLESVAVHEIGHLLGLGHSSVE SIM
Sbjct: 244 AFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIM 303

Query: 307 FPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGA 354
           +PTI +  RKVDL  DDV GVQ LYG NPN + S +   PP S Q+RDT  +GA
Sbjct: 304 YPTIRTGRRKVDLTTDDVEGVQYLYGANPNFNGSRS---PPPSTQQRDTGDSGA 354

BLAST of Moc08g30000 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 9.1e-108
Identity = 206/354 (58.19%), Postives = 248/354 (70.06%), Query Frame = 0

Query: 6   FIVLLLISLSLFSPPIINAHLFPNASAI-PSWLNNTKCLWDQFQKLKGCRPGDRLQGLSS 65
           F V   +SL L   P  +A  FPN++A+ PS  N T+  WD F    GC  G  + GL  
Sbjct: 3   FCVFGFLSLFLIVSP-ASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYR 62

Query: 66  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 125
           +K YF+ FGYIP    + NFTD FDDIL +A+  YQ NFNLNV+GELD  T++ I +PRC
Sbjct: 63  IKKYFQRFGYIPE-TFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRC 122

Query: 126 GVADIINGSSSMNSGNPAAHAASGS-ARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQ 185
           G  D++NG+S M+ G       + S    H+V  Y+ FPG+PRWP  RRDLTYAF P+N 
Sbjct: 123 GNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNP 182

Query: 186 LSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHA 245
           L+ EVK+VF RAFGRW+ VT L FT  +SF  +DI IGF+ GDHGDGEPFDGVLGTLAHA
Sbjct: 183 LTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHA 242

Query: 246 FSPPSGHFHLDGDESWVVSGDLGT--APAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 305
           FSPPSG FHLD DE+WVVSGDL +  +  AA+DLESVAVHEIGHLLGLGHSSVEESIM+P
Sbjct: 243 FSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYP 302

Query: 306 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQ-ERDTSKNGAA 355
           TIT+  RKVDL  DDV G+Q LYG NPN +    TT PPS+ + +RDT    AA
Sbjct: 303 TITTGKRKVDLTNDDVEGIQYLYGANPNFNG---TTSPPSTTKHQRDTGGFSAA 351

BLAST of Moc08g30000 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.8e-101
Identity = 193/358 (53.91%), Postives = 236/358 (65.92%), Query Frame = 0

Query: 8   VLLLISLSLFSPPIINAHLFPNASAIP--SWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 67
           +LL I +  F+   I+A  + N S+IP   +LN T+  W+ F KL GC  G+ + GLS L
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 68  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 127
           K YF+ FGYI       N TD FDD+L SA+ TYQKNFNL V+G+LD  TLRQI  PRCG
Sbjct: 64  KQYFRRFGYI---TTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 123

Query: 128 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 187
             D+I+G S MN G              +   YSFFPGKPRWP  +RDLTYAFAP+N L+
Sbjct: 124 NPDLIDGVSEMNGGK----------ILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLT 183

Query: 188 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 247
            EVK VF RAF RWA VTPL FT  +S   ADI IGFF+G+HGDGEPFDG +GTLAHA S
Sbjct: 184 DEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASS 243

Query: 248 PPSGHFHLDGDESWVVS-GDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 307
           PP+G  HLDGDE W++S G++          +DLESVAVHEIGHLLGLGHSSVE++IMFP
Sbjct: 244 PPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFP 303

Query: 308 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATLLSL 360
            I+   RKV+LA DD+ G+Q LYGGNPNGD   +     S     D+ +     ++SL
Sbjct: 304 AISGGDRKVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQSTGGDSVRRWRGWMISL 348

BLAST of Moc08g30000 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.8e-75
Identity = 147/317 (46.37%), Postives = 195/317 (61.51%), Query Frame = 0

Query: 44  WDQFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPNSN-FTDLFDDILHSALTTYQKN 103
           W  F +L   + G  + G+S LK Y   FGY+   N  S  F+D+FD  L SA++ YQ+N
Sbjct: 52  WHDFSRLVDVQIGSHVSGVSELKRYLHRFGYV---NDGSEIFSDVFDGPLESAISLYQEN 111

Query: 104 FNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFP 163
             L ++G LD  T+  + LPRCGV+D               H    +   H+ +HY++F 
Sbjct: 112 LGLPITGRLDTSTVTLMSLPRCGVSD--------------THMTINNDFLHTTAHYTYFN 171

Query: 164 GKPRWPAGRRDLTYAFAPENQL----SAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADI 223
           GKP+W   R  LTYA +  ++L    S +VK VF RAF +W++V P++F EV  F  AD+
Sbjct: 172 GKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADL 231

Query: 224 RIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESV 283
           +IGF+AGDHGDG PFDGVLGTLAHAF+P +G  HLD  E+W+V  DL  +   A+DLESV
Sbjct: 232 KIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESV 291

Query: 284 AVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTM 343
           A HEIGHLLGLGHSS E ++M+P++  RT+KVDL  DDVAGV +LYG NP          
Sbjct: 292 ATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPNPK-------LR 342

Query: 344 PPSSVQERDTSKNGAAT 356
             S  Q  D+ KNG  +
Sbjct: 352 LDSLTQSEDSIKNGTVS 342

BLAST of Moc08g30000 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.9e-74
Identity = 149/308 (48.38%), Postives = 190/308 (61.69%), Query Frame = 0

Query: 24  AHLFPNASAIPSWLNNTKCLWD--QFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPN 83
           A L+  A+++PS   +    WD     K     PG   +GLS++K YF H GYIPN    
Sbjct: 14  ATLYFLATSLPSVSAHGPYAWDGEATYKFTTYHPGQNYKGLSNVKNYFHHLGYIPNA--- 73

Query: 84  SNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNP 143
            +F D FDD L SA+ TYQKN+NLNV+G+ D +TL+QI  PRCGV DII  ++   S   
Sbjct: 74  PHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDIIINTNKTTS--- 133

Query: 144 AAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLSAEVKAVFGRAFGRWAA 203
                     F  +S Y+FF   PRW AG   LTYAF+PE +L    K+   RAF +W  
Sbjct: 134 ----------FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTP 193

Query: 204 VTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVV 263
           V  + F E  S+  A+I+I F + +HGD  PFDG  G L HAF+P  G  H D DE WV 
Sbjct: 194 VVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVA 253

Query: 264 SGDLGTAP-AAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGV 323
           SGD+  +P  +A DLESVAVHEIGHLLGLGHSS   +IM+P+I  RTRKV+LA DD+ G+
Sbjct: 254 SGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGI 305

Query: 324 QELYGGNP 329
           ++LYG NP
Sbjct: 314 RKLYGINP 305

BLAST of Moc08g30000 vs. ExPASy TrEMBL
Match: A0A6J1KK37 (metalloendoproteinase 2-MMP OS=Cucurbita maxima OX=3661 GN=LOC111493905 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.4e-159
Identity = 286/372 (76.88%), Postives = 316/372 (84.95%), Query Frame = 0

Query: 7   IVLLLISLSLFS-PPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 66
           ++L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCR GD L GLS+L
Sbjct: 6   MILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRHGDHLTGLSNL 65

Query: 67  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 126
           KTY   FGYI  PN  SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG
Sbjct: 66  KTYLNKFGYI--PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCG 125

Query: 127 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 186
           +ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+
Sbjct: 126 IADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 185

Query: 187 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 246
            EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 186 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 245

Query: 247 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 306
           PPSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 246 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305

Query: 307 RTRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTSKNGAATLLSLWWGP 366
           RTRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD SK GA  +L+ WW P
Sbjct: 306 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSKGGAPKVLNSWWSP 365

Query: 367 LLLTAVELFLMR 376
           L++ AVELFLMR
Sbjct: 366 LVVAAVELFLMR 372

BLAST of Moc08g30000 vs. ExPASy TrEMBL
Match: A0A6J1G2R8 (metalloendoproteinase 5-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111450264 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.1e-159
Identity = 287/373 (76.94%), Postives = 317/373 (84.99%), Query Frame = 0

Query: 7   IVLLLISLSLFS-PPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 66
           ++L L SLS+ + P  I+AH+FPNAS+IPSWLN TK  WDQFQ LKGCRPGD L GLS+L
Sbjct: 6   LILALFSLSILAHPHPISAHIFPNASSIPSWLNRTKYAWDQFQNLKGCRPGDHLNGLSNL 65

Query: 67  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 126
           KTY   FGYI  PN  SNF+DLFD++L SAL TYQKNFNLN +G LDD TL QIQLPRCG
Sbjct: 66  KTYLNKFGYI--PNSPSNFSDLFDNVLQSALLTYQKNFNLNATGFLDDQTLAQIQLPRCG 125

Query: 127 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 186
           +ADIING+SSMNSG+P AH A+   RFHSVSHYSFFPG+PRWP  RRDLTYAFAPEN L+
Sbjct: 126 IADIINGASSMNSGSPVAHNAT---RFHSVSHYSFFPGRPRWPDHRRDLTYAFAPENGLT 185

Query: 187 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 246
            EVK VF RAF RWAAVTPLTFT V+SFRAADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 186 DEVKGVFTRAFARWAAVTPLTFTAVESFRAADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 245

Query: 247 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 306
           PPSGHFHLDGDESWVV+GDL +AP  AIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 246 PPSGHFHLDGDESWVVTGDLRSAPVTAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305

Query: 307 RTRKVDLAADDVAGVQELYGGNPNGDNS--TTTTMPPSSVQERDTS-KNGAATLLSLWWG 366
           RTRKVDLA DDV G+QELYG NPNG+ S  ++TT PPSSVQERD S K GA  +L+ WW 
Sbjct: 306 RTRKVDLAVDDVTGIQELYGANPNGNPSPPSSTTTPPSSVQERDMSRKGGAPKVLNSWWS 365

Query: 367 PLLLTAVELFLMR 376
           PL++ AVELFLMR
Sbjct: 366 PLVVAAVELFLMR 373

BLAST of Moc08g30000 vs. ExPASy TrEMBL
Match: A0A5D3DJB2 (Metalloendoproteinase 2-MMP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00360 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.1e-155
Identity = 279/371 (75.20%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 6   FIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 65
           F+++  +SL +F  P I+A +FPN S+IPSWLN T C WDQF+KLKGCRPGDRL GLS+L
Sbjct: 3   FLLITSLSLLVFLNP-ISADIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRLNGLSNL 62

Query: 66  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 125
           KTY  HFGYIP   P+ NFTDLFD +L SAL TYQ NFNLN +G LDD TL QIQLPRCG
Sbjct: 63  KTYLNHFGYIP---PSPNFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCG 122

Query: 126 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 185
           + DIIN S+SMNSG  + H       FHSVSHY+FFPG+P WPA RRDLTYAFAPEN LS
Sbjct: 123 IPDIINNSTSMNSGRHSPH-------FHSVSHYTFFPGRPIWPAHRRDLTYAFAPENPLS 182

Query: 186 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 245
            EVKAVF RAF RWAAVTPLTF+ V+SFR ADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 183 NEVKAVFARAFARWAAVTPLTFSAVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 242

Query: 246 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305
           PPSGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 243 PPSGHFHLDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 302

Query: 306 RTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAA-TLLSLWWGPL 365
           RTRKVDLAADD+ G+QELYGGNPNG+ S  ++    SVQERD S+NGAA  LL  WW PL
Sbjct: 303 RTRKVDLAADDINGIQELYGGNPNGNLSPPSSSMTPSVQERDMSRNGAAHRLLGFWWSPL 362

Query: 366 LLTAVELFLMR 376
           L+ AVELFLMR
Sbjct: 363 LIAAVELFLMR 362

BLAST of Moc08g30000 vs. ExPASy TrEMBL
Match: A0A1S3CPI6 (metalloendoproteinase 2-MMP OS=Cucumis melo OX=3656 GN=LOC103503333 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.1e-155
Identity = 279/371 (75.20%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 6   FIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 65
           F+++  +SL +F  P I+A +FPN S+IPSWLN T C WDQF+KLKGCRPGDRL GLS+L
Sbjct: 3   FLLITSLSLLVFLNP-ISADIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRLNGLSNL 62

Query: 66  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 125
           KTY  HFGYIP   P+ NFTDLFD +L SAL TYQ NFNLN +G LDD TL QIQLPRCG
Sbjct: 63  KTYLNHFGYIP---PSPNFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCG 122

Query: 126 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 185
           + DIIN S+SMNSG  + H       FHSVSHY+FFPG+P WPA RRDLTYAFAPEN LS
Sbjct: 123 IPDIINNSTSMNSGRHSPH-------FHSVSHYTFFPGRPIWPAHRRDLTYAFAPENPLS 182

Query: 186 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 245
            EVKAVF RAF RWAAVTPLTF+ V+SFR ADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 183 NEVKAVFARAFARWAAVTPLTFSAVESFRTADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 242

Query: 246 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305
           PPSGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 243 PPSGHFHLDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 302

Query: 306 RTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAA-TLLSLWWGPL 365
           RTRKVDLAADD+ G+QELYGGNPNG+ S  ++    SVQERD S+NGAA  LL  WW PL
Sbjct: 303 RTRKVDLAADDINGIQELYGGNPNGNLSPPSSSMTPSVQERDMSRNGAAHRLLGFWWSPL 362

Query: 366 LLTAVELFLMR 376
           L+ AVELFLMR
Sbjct: 363 LIAAVELFLMR 362

BLAST of Moc08g30000 vs. ExPASy TrEMBL
Match: A0A0A0KRY9 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G484630 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 3.0e-154
Identity = 277/371 (74.66%), Postives = 307/371 (82.75%), Query Frame = 0

Query: 6   FIVLLLISLSLFSPPIINAHLFPNASAIPSWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 65
           F+++  +SL LF  P I+AH+FPN S+IPSWLN T C WDQF+KLKGCRPGDR  GLS+L
Sbjct: 3   FLLITSLSLLLFLNP-ISAHIFPNVSSIPSWLNKTTCAWDQFKKLKGCRPGDRFNGLSNL 62

Query: 66  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 125
           KTY   FGYIP+   + NFTDLFD +L SAL TYQ NFNLN +G LDD TL QIQLPRCG
Sbjct: 63  KTYLNRFGYIPS---SPNFTDLFDPLLQSALKTYQTNFNLNATGLLDDQTLAQIQLPRCG 122

Query: 126 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 185
           + DIIN S+SMNSG P       S  FHSVSHYSFFPG+P WPA RRDLTYAFAPEN LS
Sbjct: 123 IPDIINNSTSMNSGQPTGRQ---SPHFHSVSHYSFFPGRPIWPAHRRDLTYAFAPENPLS 182

Query: 186 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 245
            EVKAVF RAF RWAAVTPLTF+ V+SFR+ADIRIGF+AGDHGDGEPFDGVLGTLAHAFS
Sbjct: 183 NEVKAVFARAFARWAAVTPLTFSAVESFRSADIRIGFYAGDHGDGEPFDGVLGTLAHAFS 242

Query: 246 PPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 305
           PPSGHFHLDGDESWVVSGDL +AP AAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS
Sbjct: 243 PPSGHFHLDGDESWVVSGDLRSAPLAAIDLESVAVHEIGHLLGLGHSSVEESIMFPTITS 302

Query: 306 RTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATL-LSLWWGPL 365
           RTRKVDLAADD+ G+QELYGGNPNG+ S  ++    SVQER+ S+NGAA   L  WW P+
Sbjct: 303 RTRKVDLAADDINGIQELYGGNPNGNLSPPSSSTTPSVQEREMSRNGAAPRPLRFWWSPV 362

Query: 366 LLTAVELFLMR 376
           L+ AVELFLMR
Sbjct: 363 LIAAVELFLMR 366

BLAST of Moc08g30000 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 402.9 bits (1034), Expect = 2.8e-112
Identity = 202/354 (57.06%), Postives = 251/354 (70.90%), Query Frame = 0

Query: 7   IVLLLISLSLFSPPIINAHLFPNASAIPSWL--NNTKCLWDQFQKLKGCRPGDRLQGLSS 66
           I + ++ L  F+P  ++A  + N+SAIP  L  N T   W+ F    GC  G +  GL  
Sbjct: 4   ICVFMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNATGNPWNSFLNFTGCHAGKKYDGLYM 63

Query: 67  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 126
           LK YF+HFGYI   N + NFTD FDDIL +A+  YQ+NF LNV+G LD+ TL+ + +PRC
Sbjct: 64  LKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRC 123

Query: 127 GVADIINGSSSMNSGNPAAHA--ASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPEN 186
           G  D++NG+S+M+SG        A    RFH+V HYSFFPG+PRWP  RRDLTYAF P N
Sbjct: 124 GNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRN 183

Query: 187 QLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAH 246
            L+ EVK+VF RAF RW  VTPLTFT V+ F  +DI IGF++G+HGDGEPFDG + TLAH
Sbjct: 184 ALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAH 243

Query: 247 AFSPPSGHFHLDGDESWVVSGDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIM 306
           AFSPP+GHFHLDG+E+W+VSG+ G    + + A+DLESVAVHEIGHLLGLGHSSVE SIM
Sbjct: 244 AFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIM 303

Query: 307 FPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGA 354
           +PTI +  RKVDL  DDV GVQ LYG NPN + S +   PP S Q+RDT  +GA
Sbjct: 304 YPTIRTGRRKVDLTTDDVEGVQYLYGANPNFNGSRS---PPPSTQQRDTGDSGA 354

BLAST of Moc08g30000 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 391.7 bits (1005), Expect = 6.5e-109
Identity = 206/354 (58.19%), Postives = 248/354 (70.06%), Query Frame = 0

Query: 6   FIVLLLISLSLFSPPIINAHLFPNASAI-PSWLNNTKCLWDQFQKLKGCRPGDRLQGLSS 65
           F V   +SL L   P  +A  FPN++A+ PS  N T+  WD F    GC  G  + GL  
Sbjct: 3   FCVFGFLSLFLIVSP-ASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYR 62

Query: 66  LKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRC 125
           +K YF+ FGYIP    + NFTD FDDIL +A+  YQ NFNLNV+GELD  T++ I +PRC
Sbjct: 63  IKKYFQRFGYIPE-TFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRC 122

Query: 126 GVADIINGSSSMNSGNPAAHAASGS-ARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQ 185
           G  D++NG+S M+ G       + S    H+V  Y+ FPG+PRWP  RRDLTYAF P+N 
Sbjct: 123 GNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNP 182

Query: 186 LSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHA 245
           L+ EVK+VF RAFGRW+ VT L FT  +SF  +DI IGF+ GDHGDGEPFDGVLGTLAHA
Sbjct: 183 LTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHA 242

Query: 246 FSPPSGHFHLDGDESWVVSGDLGT--APAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 305
           FSPPSG FHLD DE+WVVSGDL +  +  AA+DLESVAVHEIGHLLGLGHSSVEESIM+P
Sbjct: 243 FSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYP 302

Query: 306 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQ-ERDTSKNGAA 355
           TIT+  RKVDL  DDV G+Q LYG NPN +    TT PPS+ + +RDT    AA
Sbjct: 303 TITTGKRKVDLTNDDVEGIQYLYGANPNFNG---TTSPPSTTKHQRDTGGFSAA 351

BLAST of Moc08g30000 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 370.2 bits (949), Expect = 2.0e-102
Identity = 193/358 (53.91%), Postives = 236/358 (65.92%), Query Frame = 0

Query: 8   VLLLISLSLFSPPIINAHLFPNASAIP--SWLNNTKCLWDQFQKLKGCRPGDRLQGLSSL 67
           +LL I +  F+   I+A  + N S+IP   +LN T+  W+ F KL GC  G+ + GLS L
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 68  KTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQLPRCG 127
           K YF+ FGYI       N TD FDD+L SA+ TYQKNFNL V+G+LD  TLRQI  PRCG
Sbjct: 64  KQYFRRFGYI---TTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 123

Query: 128 VADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRDLTYAFAPENQLS 187
             D+I+G S MN G              +   YSFFPGKPRWP  +RDLTYAFAP+N L+
Sbjct: 124 NPDLIDGVSEMNGGK----------ILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLT 183

Query: 188 AEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPFDGVLGTLAHAFS 247
            EVK VF RAF RWA VTPL FT  +S   ADI IGFF+G+HGDGEPFDG +GTLAHA S
Sbjct: 184 DEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASS 243

Query: 248 PPSGHFHLDGDESWVVS-GDLG---TAPAAAIDLESVAVHEIGHLLGLGHSSVEESIMFP 307
           PP+G  HLDGDE W++S G++          +DLESVAVHEIGHLLGLGHSSVE++IMFP
Sbjct: 244 PPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFP 303

Query: 308 TITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTMPPSSVQERDTSKNGAATLLSL 360
            I+   RKV+LA DD+ G+Q LYGGNPNGD   +     S     D+ +     ++SL
Sbjct: 304 AISGGDRKVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQSTGGDSVRRWRGWMISL 348

BLAST of Moc08g30000 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.5e-76
Identity = 147/317 (46.37%), Postives = 195/317 (61.51%), Query Frame = 0

Query: 44  WDQFQKLKGCRPGDRLQGLSSLKTYFKHFGYIPNPNPNSN-FTDLFDDILHSALTTYQKN 103
           W  F +L   + G  + G+S LK Y   FGY+   N  S  F+D+FD  L SA++ YQ+N
Sbjct: 52  WHDFSRLVDVQIGSHVSGVSELKRYLHRFGYV---NDGSEIFSDVFDGPLESAISLYQEN 111

Query: 104 FNLNVSGELDDDTLRQIQLPRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFP 163
             L ++G LD  T+  + LPRCGV+D               H    +   H+ +HY++F 
Sbjct: 112 LGLPITGRLDTSTVTLMSLPRCGVSD--------------THMTINNDFLHTTAHYTYFN 171

Query: 164 GKPRWPAGRRDLTYAFAPENQL----SAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADI 223
           GKP+W   R  LTYA +  ++L    S +VK VF RAF +W++V P++F EV  F  AD+
Sbjct: 172 GKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADL 231

Query: 224 RIGFFAGDHGDGEPFDGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESV 283
           +IGF+AGDHGDG PFDGVLGTLAHAF+P +G  HLD  E+W+V  DL  +   A+DLESV
Sbjct: 232 KIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESV 291

Query: 284 AVHEIGHLLGLGHSSVEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPNGDNSTTTTM 343
           A HEIGHLLGLGHSS E ++M+P++  RT+KVDL  DDVAGV +LYG NP          
Sbjct: 292 ATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPNPK-------LR 342

Query: 344 PPSSVQERDTSKNGAAT 356
             S  Q  D+ KNG  +
Sbjct: 352 LDSLTQSEDSIKNGTVS 342

BLAST of Moc08g30000 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-65
Identity = 133/276 (48.19%), Postives = 172/276 (62.32%), Query Frame = 0

Query: 62  LSSLKTYFKHFGYIPNPNPNSNFTDLFDDILHSALTTYQKNFNLNVSGELDDDTLRQIQL 121
           +  +K + + +GY+P  N  S+     D     AL  YQKN  L ++G+ D DTL QI L
Sbjct: 50  IPEIKRHLQQYGYLPQ-NKESD-----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILL 109

Query: 122 PRCGVADIINGSSSMNSGNPAAHAASGSARFHSVSHYSFFPGKPRWPAGRRD----LTYA 181
           PRCG  D +                  +A FH+   Y +FPG+PRW    RD    LTYA
Sbjct: 110 PRCGFPDDVEPK---------------TAPFHTGKKYVYFPGRPRW---TRDVPLKLTYA 169

Query: 182 FAPEN----QLSAEVKAVFGRAFGRWAAVTPLTFTEVQSFRAADIRIGFFAGDHGDGEPF 241
           F+ EN        +++ VF RAFG+WA+V P++F E + +  ADI+IGFF GDHGDGEPF
Sbjct: 170 FSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPF 229

Query: 242 DGVLGTLAHAFSPPSGHFHLDGDESWVVSGDLGTAPAAAIDLESVAVHEIGHLLGLGHSS 301
           DGVLG LAH FSP +G  HLD  E+W V  D     + A+DLESVAVHEIGH+LGLGHSS
Sbjct: 230 DGVLGVLAHTFSPENGRLHLDKAETWAVDFD-EEKSSVAVDLESVAVHEIGHVLGLGHSS 289

Query: 302 VEESIMFPTITSRTRKVDLAADDVAGVQELYGGNPN 330
           V+++ M+PT+  R++KV+L  DDV GVQ LYG NPN
Sbjct: 290 VKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTNPN 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022999578.14.9e-15976.88metalloendoproteinase 2-MMP [Cucurbita maxima][more]
XP_022946065.16.4e-15976.94metalloendoproteinase 5-MMP-like [Cucurbita moschata][more]
KAG7029995.18.4e-15976.94Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_023546725.14.1e-15876.68metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo][more]
XP_038889498.12.7e-15776.15metalloendoproteinase 2-MMP [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q5XF514.0e-11157.06Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
O045299.1e-10858.19Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q9ZUJ52.8e-10153.91Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
O235077.8e-7546.37Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
P291363.9e-7448.38Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KK372.4e-15976.88metalloendoproteinase 2-MMP OS=Cucurbita maxima OX=3661 GN=LOC111493905 PE=3 SV=... [more]
A0A6J1G2R83.1e-15976.94metalloendoproteinase 5-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111450264 P... [more]
A0A5D3DJB22.1e-15575.20Metalloendoproteinase 2-MMP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3CPI62.1e-15575.20metalloendoproteinase 2-MMP OS=Cucumis melo OX=3656 GN=LOC103503333 PE=3 SV=1[more]
A0A0A0KRY93.0e-15474.66ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G484630 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G24140.12.8e-11257.06Matrixin family protein [more]
AT1G70170.16.5e-10958.19matrix metalloproteinase [more]
AT1G59970.12.0e-10253.91Matrixin family protein [more]
AT4G16640.15.5e-7646.37Matrixin family protein [more]
AT2G45040.11.2e-6548.19Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 192..207
score: 52.52
coord: 278..303
score: 56.85
coord: 216..244
score: 61.56
coord: 119..132
score: 44.6
coord: 312..325
score: 58.8
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 163..326
e-value: 3.9E-46
score: 169.3
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 52..331
e-value: 3.2E-77
score: 261.3
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 62..118
e-value: 2.9E-9
score: 37.0
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 171..325
e-value: 1.1E-47
score: 161.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..348
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 324..348
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 5..357
NoneNo IPR availablePANTHERPTHR10201:SF260MATRIX METALLOPROTEINASEcoord: 5..357
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 160..328
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 166..325
e-value: 6.86401E-69
score: 212.064
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 54..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g30000.1Moc08g30000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity