Moc08g22340 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g22340
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRNase H domain-containing protein
Locationchr8: 16401932 .. 16405795 (+)
RNA-Seq ExpressionMoc08g22340
SyntenyMoc08g22340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCAGAGTCAGCAATTAAGAGAACACTCATTTACTCCCCTAAAGTCGGGGAAGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGTTCCCCACTCGGTCTCATCCCCAAAATGGTAGGAATATTGAGTCGGCAACTCGGACCATTCTCACCCATACAGATCAAAAGACGAGCCCTCAGGGGCAGGAGTCCATAACTCACTCAAGATTAAGAATGAGTTGCCTGGTCATCCTAAAAAATAGCAATCTGTTAGTTAACGGTGTTACATCTAAAGATTGGCTATTTCGCGGTCCGGTCTTATACAAACTCATTGTATAGGATACCCCCACGTGCATGTCTCCACATGAACGACCTAGATCGAGTCGTCTGTAACCTTTACAGAGTTACAGAGTGGGCTGTATCCATAGTGTTGCCAGGATAAGGTACCCAGCCTTATCCATATACTATAGACCCTTCAGGTTATTCACGGGACATTATCCATTCATATATCAGGGGTATATCCAATATATACCTCGTATATCAAATATATACCCAGTATATATACTGTCCCGATTACATGATAATAACCTTGGATGTTTAGTTTATTGGATTTTGGACAATGCAATGCTATGAATTCAACAACAAATTTATTGAATAAAACTTTATTGAATATACATTTGTTTTTACATAAATACAAACTACGAGTTTAGGGCGACACACCCAACACCCTTTAGGTTATTCATGGGACATTATCCACTCGTATATCAGGGATATATCAAATATATACCTCGTATATCAGGGGTATATCTACCCAGTATATCAAATATATATACCCAATATATATACTGTCCCGTACATGATAATAACCTTGGATGTTTAGTTTATTGGATTTGGGACAATACAATGCTATGAATTCAACAACACAACTTTATTGAATATACATTTGTTTTTACATAAATACAAACTACGAGTTTATGGCAACACACCCAAAATCATCACGGCTCAATGATCTTACCTCTTGATATTAAAAGTGACTGGATTAGCTCTTATTCTCCAAATTTTCTTGAAGCAACTGCACAGAAATTTCATTCACATCAAGCTCGGGTTACCTCTCTCTCTGTGGTTCCAGATCGCTGGCATCCACCGTCGGTCAATTGCGTGAAATTAAATACAGATGCCGCTTTTCGAAGCTTGTCCGCTTGTTTATGAATGGCGATTCAGGCTATAAAGGCTATTTGGTTGCGACAAAGACAACTCACCTCCCGCGATCTCTCTCTCCTCTTCTTGCTGAACTTCAGAGCATTCTAGAAGGCCCGAAATTCGCAAGATCCACGAATTCCATGCATCTTGAGGTTGAATCAGACTCTTTGACGGTAATTTGTTTGATTCTTGGGGACTTGCAAATAATGGGAGACGAGTTGAGTTGGCTATTTGAAATTCAATCGCTTGCTGTAGCTTTTGAAACCATCACTTTCTCCTTTACCCCTCGTTGCTGTAATCGGTGTGCTCATTCCTTGGAAAAATGGGAGGTCTCCACACGTGTGTTTAGCACTCCACTTTCCCTCCTTGGCTTTCAGATCATGTACGCGGTGATTACCCTTAAATTATTGCCCATGTGGCTTCTTAATGAAAAGTTCCATTTAAAAAAAAAAAGACAAAACTCACCTTTTATTTTATGATTTAAATTAAATGTTAAATCTTAAAATATCTCATAATGTTTAAAATTAAATAAGCAATCATAGATATTGTCCTATTTTATCTAACAAATCGACACATTCATAATATTAATGAAGCTTTGGGTAAATGATTTGGTTAAATTAATATAATTACTCAATATCTTATTTTAATCGAGAAATTTATTATCAATTCGTGTAACGCCCCAGAACTTCCCACACACTTCTAGGTCACGTTAACTACCCAACTGTCTCAACTTAACCCTAAACACGAGATGTCAACTCCATGTGAAATAATTATATAAAATCAAGCTTCAACTCTCATCAATTTAACAATGAATCAACTCTATAAAATATCAATCAACATCATCCGTGGACAACCTCATGAGGCAACTTTATTATTCCAACTAGGCAAAACTCAATGAATGGAAGAAAACTAAATAAACACCTATCCCGAAATAAAATAAACAACAAGTGGGAACCTCCCAATTATTCAAATAAGAACAAATAGTTTATTGACCAAAACATAAGGTCTTAACCATCTAAAACTTAAGCTAAGCAAAAGTAACTACGCAGCCTATGGTCCTCGCTCGTCATGTCTTCTCCGTCTCATCACCATGGTCGCCAACGAATCCTTCACCTGAAACAATAATTGTAGAGGGGGGGTGAGTATATAAAATATACTCCGTAAGTAGCCCTCACCAACAACTCGCACACATCATGCTCAACAAAATCATGAAACATTCATAGTCATGCACTTCATCACATTATGTAATCAAACATAGACACACTCTATAAGATTCGGCGACAACCACAACCATGAACAAATTAGCTACCAACCTCGAGTCTCCCTAACATCTCAAGTACCTCTACGCTTATCACAGCTATGCTAGCCTTCCACAGCTAACTCTTTCGAGTAGAGTCGGCCCCTGACCATTTTACCCAGAGCCCCGACACTATTGCATCGGCCCTCGACCATTTTTACCCAAGGCCCAGATGACTTCACCATCTATTTGCCCTTGACCATTTTTACCCAAGGCCCCAATAGTTTTCCTTCCAAAAGACTCCTCGCATCGACCCTCGACCATTTTTACCCGAGGCCTCGATACTCCATTAGTGACGTATCAGCCCTCGACCATTTATACCCGAGGCCCTGACACTCACCCATTCACCTTCCCCAAGAATCCAACGTTCCTTGTCATAACCAACTTCACATCAACCCCTAGCCTGCGATCCGGGTCCGAAAATATGCTTCAATAACACCCACATCTCATAAGAAAATAATATGACTCAGACCAGCACGCTCGCATCAGAAAGAAACATTTATATAAACTATACAAGCACGCTCACATCATAAGAAAGCATAATCACAATAACTTTATTATAACGTGCATCCTTTGTAAACACTCATCATCCCACAACCATTCTCAGCAGTTTAACAAGGCAAAATTTCCACCATTATCACCAACGAATATTCAACACTACACCAAGTATACAGATTAACACAACCGAGAGCTACCTAACACAACAGCGTATTAAAAATAATATCAAACATCACAAAAACAGCACATTAGAAGCCATCAGACACACATTAACGCACGCACACGCCCCTTCTCTACTTACTCACAACCGCGCCCCTAAGTTTTGCGTCGATCTCACACCTAGGCACTAAAACAGAAAAAAAAACAGCAAGTTACATATACTATTCAGCCTTCGAAATCAATTCTAGGACGGTTGAAATGACTCATTACCACAAAATTGACTTCGTATAGGTGTGGGTAACCTAGATATAGCTCGCCAAGGTGTGGGTAGCCTAAATCGGGCTTCGAACGGTTGTGGGTAAACCCGAATGTCACTGAAACGCTGCTGTCCACGAGTTGCAGCAGAGGGGATGCACCAAATCAGCTACCGAACACTGCTGTACCGGAGCCTAGGTGAAGTGTTTGGACCCGGAGCCGTCAGGAGCCGTTCCCAAATGACGATCTGTCACGAATGACCCTCGGACCGTCGCTGTTGCTGCAGAATCCGCGCGTCACCACCGTTCGGGTTTGGTCCGGATCGAAGATGGTCGCCGGGACGTCGGGGTGCTGTCGTCGCCGGCCGCTGCTGCTATGGATCGGGACGTCGGGGTGCTGCCGTCGCTGCCGCTGCTGTTGTAGATTGGGATGTCGGGGTGCTGCCGTAA

mRNA sequence

ATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCAGAGTCAGCAATTAAGAGAACACTCATTTACTCCCCTAAAGTCGGGGAAGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGTTCCCCACTCGGTCTCATCCCCAAAATGGTAGGAATATTGAGTCGGCAACTCGGACCATTCTCACCCATACAGATCAAAAGACGAGCCCTCAGGGGCAGGAATCGCTGGCATCCACCGTCGGTCAATTGCGTGAAATTAAATACAGATGCCGCTTTTCGAAGCTTGTCCGCTTGTTTATGAATGGCGATTCAGGCTATAAAGGCTATTTGGTTGCGACAAAGACAACTCACCTCCCGCGATCTCTCTCTCCTCTTCTTGCTGAACTTCAGAGCATTCTAGAAGGCCCGAAATTCGCAAGATCCACGAATTCCATGCATCTTGAGGTTGAATCAGACTCTTTGACGGTAATTTGTTTGATTCTTGGGGACTTGCAAATAATGGGAGACGAGTTGAGTTGGCTATTTGAAATTCAATCGCTTGCTGTAGCTTTTGAAACCATCACTTTCTCCTTTACCCCTCGTTGCTGTAATCGGTGTGCTCATTCCTTGGAAAAATGGGAGGTCTCCACACGTGTGTTTAGCACTCCACTTTCCCTCCTTGGCTTTCAGATCATCAGAGGGGATGCACCAAATCAGCTACCGAACACTGCTGTACCGGAGCCTAGAATCCGCGCGTCACCACCGTTCGGGTTTGGTCCGGATCGAAGATGGTCGCCGGGACGTCGGGGTGCTGTCGTCGCCGGCCGCTGCTGCTATGGATCGGGACGTCGGGGTGCTGCCGTCGCTGCCGCTGCTGTTGTAGATTGGGATGTCGGGGTGCTGCCGTAA

Coding sequence (CDS)

ATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCAGAGTCAGCAATTAAGAGAACACTCATTTACTCCCCTAAAGTCGGGGAAGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGTTCCCCACTCGGTCTCATCCCCAAAATGGTAGGAATATTGAGTCGGCAACTCGGACCATTCTCACCCATACAGATCAAAAGACGAGCCCTCAGGGGCAGGAATCGCTGGCATCCACCGTCGGTCAATTGCGTGAAATTAAATACAGATGCCGCTTTTCGAAGCTTGTCCGCTTGTTTATGAATGGCGATTCAGGCTATAAAGGCTATTTGGTTGCGACAAAGACAACTCACCTCCCGCGATCTCTCTCTCCTCTTCTTGCTGAACTTCAGAGCATTCTAGAAGGCCCGAAATTCGCAAGATCCACGAATTCCATGCATCTTGAGGTTGAATCAGACTCTTTGACGGTAATTTGTTTGATTCTTGGGGACTTGCAAATAATGGGAGACGAGTTGAGTTGGCTATTTGAAATTCAATCGCTTGCTGTAGCTTTTGAAACCATCACTTTCTCCTTTACCCCTCGTTGCTGTAATCGGTGTGCTCATTCCTTGGAAAAATGGGAGGTCTCCACACGTGTGTTTAGCACTCCACTTTCCCTCCTTGGCTTTCAGATCATCAGAGGGGATGCACCAAATCAGCTACCGAACACTGCTGTACCGGAGCCTAGAATCCGCGCGTCACCACCGTTCGGGTTTGGTCCGGATCGAAGATGGTCGCCGGGACGTCGGGGTGCTGTCGTCGCCGGCCGCTGCTGCTATGGATCGGGACGTCGGGGTGCTGCCGTCGCTGCCGCTGCTGTTGTAGATTGGGATGTCGGGGTGCTGCCGTAA

Protein sequence

MRGWGPLFKSQSQQLREHSFTPLKSGKSEFHLVKLCSQFPTRSHPQNGRNIESATRTILTHTDQKTSPQGQESLASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEGPKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRCCNRCAHSLEKWEVSTRVFSTPLSLLGFQIIRGDAPNQLPNTAVPEPRIRASPPFGFGPDRRWSPGRRGAVVAGRCCYGSGRRGAAVAAAAVVDWDVGVLP
Homology
BLAST of Moc08g22340 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 105.1 bits (261), Expect = 1.0e-18
Identity = 72/212 (33.96%), Postives = 103/212 (48.58%), Query Frame = 0

Query: 44   HPQNGRNIESATRTILTHTDQKTSPQGQESLASTVGQLREIKYRCRFSKLVRLFMNGDSG 103
            H +    +E     +    D  +  Q       T    R +    R S  V L +N D+ 
Sbjct: 1250 HGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAA 1309

Query: 104  YKG--------------YLVATKTTHLPRSLSPLLAELQSILEGPKFARSTNSMHLEVES 163
             +G               LVA  +  +P  LSPLLAE++ ILEG KFA ++N  HLEVES
Sbjct: 1310 CRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEVES 1369

Query: 164  DSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRCCNRCAHSLEKWEVSTR 223
            DSL  I LI  ++   GDE +W+ EIQ+L   F  I+FS + R CNR AH L KW +++ 
Sbjct: 1370 DSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGLAKWGITSP 1429

Query: 224  ------VFSTPLSLLGFQIIRGDAPNQLPNTA 236
                  +F+ P  LL   +++ D P+   + A
Sbjct: 1430 SATYAWLFNFPTWLL--DLVQRDFPSNFAHVA 1459

BLAST of Moc08g22340 vs. NCBI nr
Match: KAF4361768.1 (hypothetical protein G4B88_012076 [Cannabis sativa])

HSP 1 Score: 59.7 bits (143), Expect = 4.9e-05
Identity = 42/129 (32.56%), Postives = 63/129 (48.84%), Query Frame = 0

Query: 106 GYLVATKTTHLPRSLSPLLAELQSILEGPKFARSTNSMHLEVESDSLTVICLILGDLQIM 165
           G LVA  T  +P  LS LLAE  +I    K A + +  ++ + SDS TV+  + G+ +I 
Sbjct: 57  GALVAAATDFIPGCLSVLLAETMAIRLALKLAETRSLQNMCIASDSQTVVKALTGNTRIN 116

Query: 166 GDELSWLFEIQSLAVA---FETITFSFTPRCCNRCAHSLEKW---EVSTRVFSTPLSLLG 225
            D   W   +    +A   F  ++F F+PR CN+ AH L  W      T V++T L    
Sbjct: 117 TD---WGLLVDDWILASKRFHNLSFIFSPRNCNKVAHCLANWGRLYHVTGVWTTVLPNCA 176

Query: 226 FQIIRGDAP 229
              ++ D P
Sbjct: 177 TTCLKADVP 182

BLAST of Moc08g22340 vs. NCBI nr
Match: KAF4358383.1 (hypothetical protein G4B88_008543 [Cannabis sativa])

HSP 1 Score: 58.9 bits (141), Expect = 8.3e-05
Identity = 40/131 (30.53%), Postives = 62/131 (47.33%), Query Frame = 0

Query: 74  LASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEG 133
           + +T   L E K  C    ++R        + G LV   T ++P  LS L+AE  +I   
Sbjct: 121 MINTDASLIEGKPGCGLGFIIR-------DHLGELVTAATEYIPGCLSVLMAETLAIRLA 180

Query: 134 PKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRC 193
            K A S +  ++ + SD+ +VI  + G  +   D    L +    +  F T++F+FTPR 
Sbjct: 181 MKLAASRSLQNILIASDNQSVITALKGQTRFNTDWGKILEDCTIASKHFNTLSFNFTPRK 240

Query: 194 CNRCAHSLEKW 205
           CN  AH L  W
Sbjct: 241 CNNVAHCLANW 244

BLAST of Moc08g22340 vs. NCBI nr
Match: KAF4363590.1 (hypothetical protein F8388_002131 [Cannabis sativa])

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-04
Identity = 40/131 (30.53%), Postives = 62/131 (47.33%), Query Frame = 0

Query: 74  LASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEG 133
           + +T   L E K  C    ++R        + G LV   T ++P  LS L+AE  +I   
Sbjct: 358 MINTDASLIEGKPGCGLGVIIR-------DHLGELVTAATDYIPGCLSVLMAETLAIRLA 417

Query: 134 PKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRC 193
            K A S +  ++ + SD+ +VI  + G  +   D    L +    +  F T++F+FTPR 
Sbjct: 418 MKLAASRSLQNIFIASDNQSVITALKGQTRFNTDWGKILEDCIIASKNFNTLSFNFTPRK 477

Query: 194 CNRCAHSLEKW 205
           CN  AH L  W
Sbjct: 478 CNNVAHCLANW 481

BLAST of Moc08g22340 vs. NCBI nr
Match: KAF4389856.1 (hypothetical protein G4B88_024137 [Cannabis sativa])

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-04
Identity = 40/131 (30.53%), Postives = 62/131 (47.33%), Query Frame = 0

Query: 74  LASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEG 133
           + +T   L E K  C    ++R        + G LV   T ++P  LS L+AE  +I   
Sbjct: 327 MINTDASLIEGKPGCGLGVIIR-------DHLGELVTAATDYIPGCLSVLMAETLAIRLA 386

Query: 134 PKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRC 193
            K A S +  ++ + SD+ +VI  + G  +   D    L +    +  F T++F+FTPR 
Sbjct: 387 MKLAASRSLQNIFIASDNQSVITALKGQTRFNTDWGKILEDCIIASKNFNTLSFNFTPRK 446

Query: 194 CNRCAHSLEKW 205
           CN  AH L  W
Sbjct: 447 CNNVAHCLANW 450

BLAST of Moc08g22340 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 4.9e-19
Identity = 72/212 (33.96%), Postives = 103/212 (48.58%), Query Frame = 0

Query: 44   HPQNGRNIESATRTILTHTDQKTSPQGQESLASTVGQLREIKYRCRFSKLVRLFMNGDSG 103
            H +    +E     +    D  +  Q       T    R +    R S  V L +N D+ 
Sbjct: 1250 HGKQVSPVEFKCEWLTPFLDSHSQAQMSNYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAA 1309

Query: 104  YKG--------------YLVATKTTHLPRSLSPLLAELQSILEGPKFARSTNSMHLEVES 163
             +G               LVA  +  +P  LSPLLAE++ ILEG KFA ++N  HLEVES
Sbjct: 1310 CRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEVES 1369

Query: 164  DSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRCCNRCAHSLEKWEVSTR 223
            DSL  I LI  ++   GDE +W+ EIQ+L   F  I+FS + R CNR AH L KW +++ 
Sbjct: 1370 DSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGLAKWGITSP 1429

Query: 224  ------VFSTPLSLLGFQIIRGDAPNQLPNTA 236
                  +F+ P  LL   +++ D P+   + A
Sbjct: 1430 SATYAWLFNFPTWLL--DLVQRDFPSNFAHVA 1459

BLAST of Moc08g22340 vs. ExPASy TrEMBL
Match: A0A7J6EJ00 (RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_008543 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.0e-05
Identity = 40/131 (30.53%), Postives = 62/131 (47.33%), Query Frame = 0

Query: 74  LASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEG 133
           + +T   L E K  C    ++R        + G LV   T ++P  LS L+AE  +I   
Sbjct: 121 MINTDASLIEGKPGCGLGFIIR-------DHLGELVTAATEYIPGCLSVLMAETLAIRLA 180

Query: 134 PKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRC 193
            K A S +  ++ + SD+ +VI  + G  +   D    L +    +  F T++F+FTPR 
Sbjct: 181 MKLAASRSLQNILIASDNQSVITALKGQTRFNTDWGKILEDCTIASKHFNTLSFNFTPRK 240

Query: 194 CNRCAHSLEKW 205
           CN  AH L  W
Sbjct: 241 CNNVAHCLANW 244

BLAST of Moc08g22340 vs. ExPASy TrEMBL
Match: A0A7J6F1G1 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_002131 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.3e-05
Identity = 40/131 (30.53%), Postives = 62/131 (47.33%), Query Frame = 0

Query: 74  LASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEG 133
           + +T   L E K  C    ++R        + G LV   T ++P  LS L+AE  +I   
Sbjct: 358 MINTDASLIEGKPGCGLGVIIR-------DHLGELVTAATDYIPGCLSVLMAETLAIRLA 417

Query: 134 PKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRC 193
            K A S +  ++ + SD+ +VI  + G  +   D    L +    +  F T++F+FTPR 
Sbjct: 418 MKLAASRSLQNIFIASDNQSVITALKGQTRFNTDWGKILEDCIIASKNFNTLSFNFTPRK 477

Query: 194 CNRCAHSLEKW 205
           CN  AH L  W
Sbjct: 478 CNNVAHCLANW 481

BLAST of Moc08g22340 vs. ExPASy TrEMBL
Match: A0A7J6H3K7 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_024137 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.3e-05
Identity = 40/131 (30.53%), Postives = 62/131 (47.33%), Query Frame = 0

Query: 74  LASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEG 133
           + +T   L E K  C    ++R        + G LV   T ++P  LS L+AE  +I   
Sbjct: 327 MINTDASLIEGKPGCGLGVIIR-------DHLGELVTAATDYIPGCLSVLMAETLAIRLA 386

Query: 134 PKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRC 193
            K A S +  ++ + SD+ +VI  + G  +   D    L +    +  F T++F+FTPR 
Sbjct: 387 MKLAASRSLQNIFIASDNQSVITALKGQTRFNTDWGKILEDCIIASKNFNTLSFNFTPRK 446

Query: 194 CNRCAHSLEKW 205
           CN  AH L  W
Sbjct: 447 CNNVAHCLANW 450

BLAST of Moc08g22340 vs. ExPASy TrEMBL
Match: A0A7J6GEF1 (RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_009591 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-04
Identity = 40/131 (30.53%), Postives = 61/131 (46.56%), Query Frame = 0

Query: 74  LASTVGQLREIKYRCRFSKLVRLFMNGDSGYKGYLVATKTTHLPRSLSPLLAELQSILEG 133
           + +T   L E K  C    ++R        + G LV   T ++P  LS L+AE  +I   
Sbjct: 358 MINTDASLIEGKPGCGLGVIIR-------DHLGELVTAATDYIPGYLSVLMAETLAIRLA 417

Query: 134 PKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVAFETITFSFTPRC 193
            K A S +  ++ + SD+ +VI  + G  +   D    L +    A  F T++F+FTPR 
Sbjct: 418 LKLAASRSLQNIFIASDNQSVITALKGQTRFNTDWGKILEDCIIAAKNFNTLSFNFTPRK 477

Query: 194 CNRCAHSLEKW 205
           CN  AH    W
Sbjct: 478 CNNVAHCFANW 481

BLAST of Moc08g22340 vs. TAIR 10
Match: AT4G09490.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 7.5e-04
Identity = 31/104 (29.81%), Postives = 50/104 (48.08%), Query Frame = 0

Query: 122 PLLAELQSILEGPKFARSTNSMHLEVESDSLTVICLILGDLQIMGDELSWLFEIQSLAVA 181
           PL+AE  ++    ++A+S     L + SDS  +I  I  +     +    +F+I +L++ 
Sbjct: 73  PLMAEAIALFLALQYAQSIGITKLSMASDSQQLITAITSE-SPSTEFYGIIFDILNLSLG 132

Query: 182 FETITFSFTPRCCNRCAHSLEKWEVSTRVFSTPLSLLGFQIIRG 226
           F  ++FSF PR  NR A  L K            SL+ F I+ G
Sbjct: 133 FADVSFSFVPRSENRVADELAK-----------SSLISFSIVPG 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.11.0e-1833.96uncharacterized protein LOC111024874 [Momordica charantia][more]
KAF4361768.14.9e-0532.56hypothetical protein G4B88_012076 [Cannabis sativa][more]
KAF4358383.18.3e-0530.53hypothetical protein G4B88_008543 [Cannabis sativa][more]
KAF4363590.11.1e-0430.53hypothetical protein F8388_002131 [Cannabis sativa][more]
KAF4389856.11.1e-0430.53hypothetical protein G4B88_024137 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DX304.9e-1933.96uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A7J6EJ004.0e-0530.53RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_008543 PE=... [more]
A0A7J6F1G15.3e-0530.53Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_002131 PE=4 SV=1[more]
A0A7J6H3K75.3e-0530.53Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_024137 PE=4 SV=1[more]
A0A7J6GEF11.2e-0430.53RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_009591 PE=... [more]
Match NameE-valueIdentityDescription
AT4G09490.17.5e-0429.81Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 98..210
e-value: 4.1E-6
score: 28.7
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 109..201
e-value: 1.6E-13
score: 50.5
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 113..201
e-value: 5.86441E-13
score: 62.3316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g22340.1Moc08g22340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity