Moc08g22250 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g22250
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	ribonuclease MC-like
Location	chr8: 16341536 .. 16342290 (+)
RNA-Seq Expression	Moc08g22250
Synteny	Moc08g22250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTCTAGCGATTTCAAGCTCAGAGGGCGTGAACGAATTCGATTACTACCAAGTCGTGCTTCAATGGCAACCGGCCACTTGTAGCAGTGCAACTCTAAAAACTGTATGCGTGCAACCTCCAGAAGATAGATATAGTGTCCATGGTGTTTGGCCTAGCCTATCGTCAGGATATCTTCCAACGTGTTCTGGTACTCCGCCCTATGATGCCAGAATGGTAGTCGTTCATATATCTTATAAATCTCGTTAGGTCCCCTAATCTTTTTAACGTGGGATGTTTATCAATCTAAATGTTTTTGTTTTGAAATTCAGATATTGACGATTCAAGCTAGGTTAGACCAAGACTGGCCAAACGTGATAACCGGACAAAACCCGCAGTTTTGGAGCTACGAGTGGACAAAGCACGGGACTTGTTCGAGCGCGTATCTGAACCAATTCCACTACTTCAATTTTGGACTATGGGTATATACCAATTACGATCTGCAATCTATTCTGTATCAATATGGTATCTACCCCAATGGACAACAATACACAAAAACAGTTGTCCAAAATGCAATTTATAGTGCAACGGGAAAGTGGCCAGCCCTTCGCTGCAACAACAACTACAGAACTGGTAACATGCAGTTGCATGAAATAAGTTTGTGTTTCAGCAAGAATGGACAGATTCTCATAGACTGCCCATTCAATGTCGTTACATGCAATGAATATTTCGTATGGAATTCATGGAGTTATTCAGCGAGTGTGGCTAAGGAGTAA

mRNA sequence

ATGTTTCTAGCGATTTCAAGCTCAGAGGGCGTGAACGAATTCGATTACTACCAAGTCGTGCTTCAATGGCAACCGGCCACTTGTAGCAGTGCAACTCTAAAAACTGTATGCGTGCAACCTCCAGAAGATAGATATAGTGTCCATGGTGTTTGGCCTAGCCTATCGTCAGGATATCTTCCAACGTGTTCTGGTACTCCGCCCTATGATGCCAGAATGATATTGACGATTCAAGCTAGGTTAGACCAAGACTGGCCAAACGTGATAACCGGACAAAACCCGCAGTTTTGGAGCTACGAGTGGACAAAGCACGGGACTTGTTCGAGCGCGTATCTGAACCAATTCCACTACTTCAATTTTGGACTATGGGTATATACCAATTACGATCTGCAATCTATTCTGTATCAATATGGTATCTACCCCAATGGACAACAATACACAAAAACAGTTGTCCAAAATGCAATTTATAGTGCAACGGGAAAGTGGCCAGCCCTTCGCTGCAACAACAACTACAGAACTGGTAACATGCAGTTGCATGAAATAAGTTTGTGTTTCAGCAAGAATGGACAGATTCTCATAGACTGCCCATTCAATGTCGTTACATGCAATGAATATTTCGTATGGAATTCATGGAGTTATTCAGCGAGTGTGGCTAAGGAGTAA

Coding sequence (CDS)

ATGTTTCTAGCGATTTCAAGCTCAGAGGGCGTGAACGAATTCGATTACTACCAAGTCGTGCTTCAATGGCAACCGGCCACTTGTAGCAGTGCAACTCTAAAAACTGTATGCGTGCAACCTCCAGAAGATAGATATAGTGTCCATGGTGTTTGGCCTAGCCTATCGTCAGGATATCTTCCAACGTGTTCTGGTACTCCGCCCTATGATGCCAGAATGATATTGACGATTCAAGCTAGGTTAGACCAAGACTGGCCAAACGTGATAACCGGACAAAACCCGCAGTTTTGGAGCTACGAGTGGACAAAGCACGGGACTTGTTCGAGCGCGTATCTGAACCAATTCCACTACTTCAATTTTGGACTATGGGTATATACCAATTACGATCTGCAATCTATTCTGTATCAATATGGTATCTACCCCAATGGACAACAATACACAAAAACAGTTGTCCAAAATGCAATTTATAGTGCAACGGGAAAGTGGCCAGCCCTTCGCTGCAACAACAACTACAGAACTGGTAACATGCAGTTGCATGAAATAAGTTTGTGTTTCAGCAAGAATGGACAGATTCTCATAGACTGCCCATTCAATGTCGTTACATGCAATGAATATTTCGTATGGAATTCATGGAGTTATTCAGCGAGTGTGGCTAAGGAGTAA

Protein sequence

MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE

Homology

[Close]

BLAST of Moc08g22250 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 477.6 bits (1228), Expect = 5.6e-131
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 60
           MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP
Sbjct: 12  MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 71

Query: 61  TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFG 120
           TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFG
Sbjct: 72  TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFG 131

Query: 121 LWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEI 180
           LWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEI
Sbjct: 132 LWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEI 191

Query: 181 SLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 220
           SLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE
Sbjct: 192 SLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

[Close]

BLAST of Moc08g22250 vs. NCBI nr
Match: XP_022156540.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 313.9 bits (803), Expect = 1.1e-81
Identity = 143/215 (66.51%), Postives = 170/215 (79.07%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 60
           M L I  SEGVNEFDYYQVVLQWQPATCSS+TL   CVQPP++R+S+HGVWPSL SG   
Sbjct: 13  MLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIHGVWPSLLSGD-A 72

Query: 61  TCSGTPPYDARMI-LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNF 120
            CSGTPPYD   +  +I+ARL QDWP+V+   +P FWS+EWT HGTCS AYL+QFHYF+F
Sbjct: 73  VCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCSYAYLDQFHYFDF 132

Query: 121 GLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHE 180
           GL+VY NYD+  IL  Y +YPNG  Y ++ V+N IYSATGKWPALRCNNN+ +G++QLHE
Sbjct: 133 GLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRCNNNFWSGDVQLHE 192

Query: 181 ISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSA 215
           ISLCF K    L DCP+N V CN+YFVWNSWS +A
Sbjct: 193 ISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSSNA 226

[Close]

BLAST of Moc08g22250 vs. NCBI nr
Match: XP_038903348.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 285.4 bits (729), Expect = 4.1e-73
Identity = 123/209 (58.85%), Postives = 160/209 (76.56%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 60
           + L + SS+G+NEFDYYQV+ QWQPA CSSATLK+ CV+ PE+R+++HGVWPSL+SG   
Sbjct: 13  LLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIHGVWPSLTSGNAT 72

Query: 61  TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFG 120
            CSGTPPYD   I  I++RLDQDWP+V+ G NP  WS+EW  HGTCS+ YL+Q++YFNFG
Sbjct: 73  KCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSNYYLDQYNYFNFG 132

Query: 121 LWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEI 180
           L V+  Y++ SIL +YGI P+G  Y+   +++AIY+AT KWPALRCN N  +G  QL+EI
Sbjct: 133 LSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNKNKSSGKKQLYEI 192

Query: 181 SLCFSKNGQILIDCPFNVVTCNEYFVWNS 210
           +LCF+K  + L DCP+N VTC   F WNS
Sbjct: 193 ALCFTKVNRALQDCPYNAVTCGNKFSWNS 221

[Close]

BLAST of Moc08g22250 vs. NCBI nr
Match: XP_022159354.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 246.9 bits (629), Expect = 1.6e-61
Identity = 113/207 (54.59%), Postives = 138/207 (66.67%), Query Frame = 0


Query: 13  EFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARM 72
           +++YYQV LQWQPATCS A L+T CV  P+DR+S+HGVWP+LSSG  P C GTP YD   
Sbjct: 20  DYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHGVWPTLSSGSYPQCPGTPAYDGSK 79

Query: 73  ILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYTNYDLQSI 132
           I TI+ RL+ DWP+VI G NP  WS+EWT HGTCS   L+Q  YF FGL  Y  YD+ S 
Sbjct: 80  ISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYPSLSQLDYFTFGLDTYAKYDIYSA 139

Query: 133 LYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQILI 192
           L +  IYP+   Y+K+ + NAI   T K P LRCN N  +   QLHEI LCF+K+ + +I
Sbjct: 140 LDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNTNDYSRERQLHEIILCFAKSNRAII 199

Query: 193 DCPFNVVTCNEYFVWNSWSYSASVAKE 220
           DCP NVVTC  Y  W  W Y   V K+
Sbjct: 200 DCPSNVVTCGNYVYWYPW-YPEGVKKQ 225

[Close]

BLAST of Moc08g22250 vs. NCBI nr
Match: XP_022149793.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 231.1 bits (588), Expect = 9.2e-57
Identity = 111/220 (50.45%), Postives = 141/220 (64.09%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 60
           M + ++  EG+NEFDY QVV+QWQPATCS    K  C Q P +R+S+HGVWPSL  GY  
Sbjct: 13  MLVLVAICEGLNEFDYLQVVIQWQPATCSDYN-KPACFQAPGNRFSIHGVWPSLYDGYPT 72

Query: 61  TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS-SAYLNQFHYFNF 120
            CSG+ P+D   I  +Q  + + WPNV TG N   W +EW KHGTCS S + +Q  YF F
Sbjct: 73  QCSGS-PFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSESTFYDQQEYFGF 132

Query: 121 GLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHE 180
           G+  Y + +LQ IL    I PNG+ YT+  V  AI + TGK PA+RCN N RTG  QLHE
Sbjct: 133 GMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYNSRTGASQLHE 192

Query: 181 ISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 220
           ISLCF K+  +  DCP N   C   FVWNSW+   ++++E
Sbjct: 193 ISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAISEE 230

[Close]

BLAST of Moc08g22250 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.6e-32
Identity = 69/194 (35.57%), Postives = 105/194 (54.12%), Query Frame = 0


Query: 14  FDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARMI 73
           FD +  V QW PA CS     + C       +++HG+WP  S   L  C    P+D   I
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGS-CPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 74  LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYTNYDLQSIL 133
             +Q++L+  WPNV+   N QFWS+EWTKHGTCS +  NQ  YF   + +  NYD+   L
Sbjct: 61  SHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 134 YQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQILID 193
             +   PNG+  ++  ++  + +  GK+P LRC  + +T    L ++  CF+++G  LID
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 194 CPFNVVTCNEYFVW 208
           C  +  TC   F++
Sbjct: 181 CTRD--TCGANFIF 191

[Close]

BLAST of Moc08g22250 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.3e-28
Identity = 66/201 (32.84%), Postives = 108/201 (53.73%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQ---PPEDRYSVHGVWPSLSSG 60
           +F A SSSE   +FD++  V QW  + C   T K  C      P   + +HG+WP+   G
Sbjct: 20  VFSASSSSE---DFDFFYFVQQWPGSYCD--TQKKCCYPNSGKPAADFGIHGLWPNYKDG 79

Query: 61  YLPT-CSGTPPYDARMILTIQARLDQDWPNVI--TGQNPQFWSYEWTKHGTCSSAYLNQF 120
             P+ C  + P+D+  I  +   + + WP +   +G    FW +EW KHGTCS + ++Q 
Sbjct: 80  TYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQH 139

Query: 121 HYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGN 180
            YF   L +    +L   L + GI P+G+ Y+   ++++I  + G  P + CN +  +GN
Sbjct: 140 EYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRD-GSGN 199

Query: 181 MQLHEISLCFSKNGQILIDCP 196
            QL+++ LC  ++G  LI+CP
Sbjct: 200 SQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of Moc08g22250 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 119.4 bits (298), Expect = 5.1e-26
Identity = 65/197 (32.99%), Postives = 108/197 (54.82%), Query Frame = 0


Query: 3   LAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPE-DRYSVHGVWPSLSSGYLPT 62
           + I  S    +FD+Y  V QW  + C S T    C+Q  E + +++HG+WP  S G  P+
Sbjct: 21  VTIYESSKPGDFDFYLFVQQWIYSYCDSQT----CIQNKEREAFTIHGLWPENSDGSYPS 80

Query: 63  CSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS-SAYLNQFH-YFNF 122
               P ++   I  ++ +L+ DWP+ +TG N  FW++E++KHGTCS +  +   H YF  
Sbjct: 81  FCSGPSFNVNAIQDLEDQLNFDWPS-LTGPNTDFWTHEFSKHGTCSITGPITDIHDYFAT 140

Query: 123 GLWVYTNYDLQSILYQYGIYPNGQQYTKTV-VQNAIYSATGKWPALRCNNNYRTGNMQLH 182
           G+ +YT +++ + L    IYP+     K V + NAI +  G  P ++C++       QL 
Sbjct: 141 GIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFGGKPGIQCSSG------QLS 200

Query: 183 EISLCFSKNGQILIDCP 196
            +++C  KN   ++DCP
Sbjct: 201 TVAVCIDKNSLSIMDCP 206

[Close]

BLAST of Moc08g22250 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 60/190 (31.58%), Postives = 96/190 (50.53%), Query Frame = 0


Query: 13  EFDYYQVVLQWQPATCSSATLKTVCVQP----PEDRYSVHGVWPSLSSGYLP-TCSGTPP 72
           +FD++  VLQW  A C S   +  C  P    P   + +HG+WP+  +G  P  C+    
Sbjct: 21  DFDFFYFVLQWPGAYCDS---RHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSR 80

Query: 73  YDARMILTIQARLDQDWPNVITGQNP--QFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYT 132
           +D   +  + + L ++WP +    N   +FW++EW KHGTC+ + L+Q  YF  GL +  
Sbjct: 81  FDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQ 140

Query: 133 NYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFS 192
             +L   L   GI P+ + Y    ++N I    G  P + CN +  + N QL++I LC  
Sbjct: 141 KANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNSQLYQIYLCVD 200

Query: 193 KNGQILIDCP 196
            +    I+CP
Sbjct: 201 TSASKFINCP 206

[Close]

BLAST of Moc08g22250 vs. ExPASy Swiss-Prot
Match: Q40965 (Ribonuclease S-2 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 6.9e-23
Identity = 73/208 (35.10%), Postives = 105/208 (50.48%), Query Frame = 0


Query: 3   LAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTC 62
           L +SSS     +DY+Q   Q+Q A C+S    T C  PP+  ++VHG+WPS   G  P  
Sbjct: 14  LILSSSAA--RYDYFQFTQQYQQAFCNSN--PTPCKDPPDKLFTVHGLWPSTKVGRDPEY 73

Query: 63  SGTPPYDARMILTIQARLDQDWPNVITGQ-NPQFWSYEWTKHGTCSSAYL-NQFHYFN-- 122
             T  Y  R I  ++ +L+  WPNV   + N  FW  +W KHG+C+S  L NQ HYF   
Sbjct: 74  CKTKRY--RKIQRLEPQLEIIWPNVSDRKANRGFWRKQWYKHGSCASPALPNQKHYFETV 133

Query: 123 FGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKW-PALRCNNNYRTGNMQL 182
             +++    ++  IL    I P G+  T   +QNAI + T    P L+C      G  +L
Sbjct: 134 IRMFLAEKQNVSRILSMATIEPEGKNRTLLEIQNAIRAGTNNMIPKLKCQK--VNGMTEL 193

Query: 183 HEISLCFSKNGQILIDCPFNVVTCNEYF 206
            E++LC   N    I+CP  +   + YF
Sbjct: 194 VEVTLCHDSNLTQFINCPRPLPQASPYF 213

[Close]

BLAST of Moc08g22250 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 2.7e-131
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 60
           MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP
Sbjct: 12  MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 71

Query: 61  TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFG 120
           TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFG
Sbjct: 72  TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFG 131

Query: 121 LWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEI 180
           LWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEI
Sbjct: 132 LWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEI 191

Query: 181 SLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 220
           SLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE
Sbjct: 192 SLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

[Close]

BLAST of Moc08g22250 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.2e-82
Identity = 143/215 (66.51%), Postives = 170/215 (79.07%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 60
           M L I  SEGVNEFDYYQVVLQWQPATCSS+TL   CVQPP++R+S+HGVWPSL SG   
Sbjct: 13  MLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIHGVWPSLLSGD-A 72

Query: 61  TCSGTPPYDARMI-LTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNF 120
            CSGTPPYD   +  +I+ARL QDWP+V+   +P FWS+EWT HGTCS AYL+QFHYF+F
Sbjct: 73  VCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCSYAYLDQFHYFDF 132

Query: 121 GLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHE 180
           GL+VY NYD+  IL  Y +YPNG  Y ++ V+N IYSATGKWPALRCNNN+ +G++QLHE
Sbjct: 133 GLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRCNNNFWSGDVQLHE 192

Query: 181 ISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSA 215
           ISLCF K    L DCP+N V CN+YFVWNSWS +A
Sbjct: 193 ISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSSNA 226

[Close]

BLAST of Moc08g22250 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 7.8e-62
Identity = 113/207 (54.59%), Postives = 138/207 (66.67%), Query Frame = 0


Query: 13  EFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLPTCSGTPPYDARM 72
           +++YYQV LQWQPATCS A L+T CV  P+DR+S+HGVWP+LSSG  P C GTP YD   
Sbjct: 20  DYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHGVWPTLSSGSYPQCPGTPAYDGSK 79

Query: 73  ILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYTNYDLQSI 132
           I TI+ RL+ DWP+VI G NP  WS+EWT HGTCS   L+Q  YF FGL  Y  YD+ S 
Sbjct: 80  ISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYPSLSQLDYFTFGLDTYAKYDIYSA 139

Query: 133 LYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQILI 192
           L +  IYP+   Y+K+ + NAI   T K P LRCN N  +   QLHEI LCF+K+ + +I
Sbjct: 140 LDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNTNDYSRERQLHEIILCFAKSNRAII 199

Query: 193 DCPFNVVTCNEYFVWNSWSYSASVAKE 220
           DCP NVVTC  Y  W  W Y   V K+
Sbjct: 200 DCPSNVVTCGNYVYWYPW-YPEGVKKQ 225

[Close]

BLAST of Moc08g22250 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 4.4e-57
Identity = 111/220 (50.45%), Postives = 141/220 (64.09%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHGVWPSLSSGYLP 60
           M + ++  EG+NEFDY QVV+QWQPATCS    K  C Q P +R+S+HGVWPSL  GY  
Sbjct: 13  MLVLVAICEGLNEFDYLQVVIQWQPATCSDYN-KPACFQAPGNRFSIHGVWPSLYDGYPT 72

Query: 61  TCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS-SAYLNQFHYFNF 120
            CSG+ P+D   I  +Q  + + WPNV TG N   W +EW KHGTCS S + +Q  YF F
Sbjct: 73  QCSGS-PFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSESTFYDQQEYFGF 132

Query: 121 GLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHE 180
           G+  Y + +LQ IL    I PNG+ YT+  V  AI + TGK PA+RCN N RTG  QLHE
Sbjct: 133 GMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYNSRTGASQLHE 192

Query: 181 ISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 220
           ISLCF K+  +  DCP N   C   FVWNSW+   ++++E
Sbjct: 193 ISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAISEE 230

[Close]

BLAST of Moc08g22250 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.9e-55
Identity = 105/220 (47.73%), Postives = 145/220 (65.91%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLK-TVCVQPPEDRYSVHGVWPSLSSGYL 60
           M + I+S EG+NEFDY QVV+QWQPATCS++     +C Q P++RYS+HGVWPSL SG  
Sbjct: 12  MLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIHGVWPSLYSGAA 71

Query: 61  PTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSAYL-NQFHYFN 120
             CSG+ P++A  I  +Q  + + WPN+I G N   W +EW KHGTC+   L +Q  YF+
Sbjct: 72  TQCSGS-PFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEEILFDQDRYFS 131

Query: 121 FGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLH 180
           F +  Y +Y+L  +L    I PNGQ Y++  V  AI + TGK PA+RCN N  TG  Q+H
Sbjct: 132 FAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCNYNRWTGEQQMH 191

Query: 181 EISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAK 219
           E+SLC++ +   ++DCP N   C+ YFVW SWS   +V++
Sbjct: 192 EVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVSE 230

[Close]

BLAST of Moc08g22250 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 125.2 bits (313), Expect = 6.6e-29
Identity = 66/201 (32.84%), Postives = 108/201 (53.73%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQ---PPEDRYSVHGVWPSLSSG 60
           +F A SSSE   +FD++  V QW  + C   T K  C      P   + +HG+WP+   G
Sbjct: 20  VFSASSSSE---DFDFFYFVQQWPGSYCD--TQKKCCYPNSGKPAADFGIHGLWPNYKDG 79

Query: 61  YLPT-CSGTPPYDARMILTIQARLDQDWPNVI--TGQNPQFWSYEWTKHGTCSSAYLNQF 120
             P+ C  + P+D+  I  +   + + WP +   +G    FW +EW KHGTCS + ++Q 
Sbjct: 80  TYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQH 139

Query: 121 HYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGN 180
            YF   L +    +L   L + GI P+G+ Y+   ++++I  + G  P + CN +  +GN
Sbjct: 140 EYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRD-GSGN 199

Query: 181 MQLHEISLCFSKNGQILIDCP 196
            QL+++ LC  ++G  LI+CP
Sbjct: 200 SQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of Moc08g22250 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 116.7 bits (291), Expect = 2.3e-26
Identity = 64/202 (31.68%), Postives = 97/202 (48.02%), Query Frame = 0


Query: 1   MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQP----PEDRYSVHGVWPSLSS 60
           +F  +  S    +FD++  VLQW  A C ++     C  P    P   + +HG+WP+ + 
Sbjct: 15  VFQGLFVSRPQEDFDFFYFVLQWPGAYCDTS---RACCYPTSGKPAADFGIHGLWPNYNG 74

Query: 61  GYLPT-CSGTPPYDARMILTIQARLDQDWPNVITGQNP--QFWSYEWTKHGTCSSAYLNQ 120
           G  P+ C     +D   I  + + L ++WP +    N    FW +EW KHGTCS + ++Q
Sbjct: 75  GSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQ 134

Query: 121 FHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTG 180
             YF   L +    +L  IL   GI P+   Y    + NAI    G  P + CN +    
Sbjct: 135 HEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPER- 194

Query: 181 NMQLHEISLCFSKNGQILIDCP 196
           N QLH+I +C   +G   I+CP
Sbjct: 195 NAQLHQIYICVDTSGTEFIECP 212

[Close]

BLAST of Moc08g22250 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 111.7 bits (278), Expect = 7.6e-25
Identity = 60/190 (31.58%), Postives = 96/190 (50.53%), Query Frame = 0


Query: 13  EFDYYQVVLQWQPATCSSATLKTVCVQP----PEDRYSVHGVWPSLSSGYLP-TCSGTPP 72
           +FD++  VLQW  A C S   +  C  P    P   + +HG+WP+  +G  P  C+    
Sbjct: 21  DFDFFYFVLQWPGAYCDS---RHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSR 80

Query: 73  YDARMILTIQARLDQDWPNVITGQNP--QFWSYEWTKHGTCSSAYLNQFHYFNFGLWVYT 132
           +D   +  + + L ++WP +    N   +FW++EW KHGTC+ + L+Q  YF  GL +  
Sbjct: 81  FDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQ 140

Query: 133 NYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFS 192
             +L   L   GI P+ + Y    ++N I    G  P + CN +  + N QL++I LC  
Sbjct: 141 KANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNSQLYQIYLCVD 200

Query: 193 KNGQILIDCP 196
            +    I+CP
Sbjct: 201 TSASKFINCP 206

[Close]

BLAST of Moc08g22250 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-23
Identity = 60/203 (29.56%), Postives = 95/203 (46.80%), Query Frame = 0


Query: 3   LAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDR----YSVHGVWPSLSSGY 62
           L +SSS+   +F+++  V  W  A C S   +  C  P +      + +HG+WP  ++G 
Sbjct: 15  LVVSSSQTEPDFNFFYWVNYWPGAICDS---QKGCCPPTKGNTASDFIIHGLWPQFNNGT 74

Query: 63  LPT-CSGTPPYDARMILTIQARLDQDWPN----VITGQNPQFWSYEWTKHGTCSSAYLNQ 122
            P  C  T  +D   I  +  ++++ W               W +EW KHGTC  +  +Q
Sbjct: 75  WPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQ 134

Query: 123 FHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNNYRTG 182
             YF   L       L +IL Q GI PN   Y+   ++NAI  A G  P + CN + + G
Sbjct: 135 HSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIECNEDVK-G 194

Query: 183 NMQLHEISLCFSKNGQILIDCPF 197
           N QL +I +C     +  ++CP+
Sbjct: 195 NKQLFQIYICLDNYAKEFVECPY 213

The following BLAST results are available for this feature:

BLAST of Moc08g22250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158939.1	5.6e-131	100.00	ribonuclease MC-like [Momordica charantia]	[more]
XP_022156540.1	1.1e-81	66.51	ribonuclease MC-like [Momordica charantia]	[more]
XP_038903348.1	4.1e-73	58.85	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022159354.1	1.6e-61	54.59	ribonuclease MC-like [Momordica charantia]	[more]
XP_022149793.1	9.2e-57	50.45	ribonuclease MC-like [Momordica charantia]	[more]

BLAST of Moc08g22250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	3.6e-32	35.57	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	9.3e-28	32.84	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
Q7M438	5.1e-26	32.99	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]
P42815	1.1e-23	31.58	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q40965	6.9e-23	35.10	Ribonuclease S-2 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of Moc08g22250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0X1	2.7e-131	100.00	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1DQK2	5.2e-82	66.51	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
A0A6J1E266	7.8e-62	54.59	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]
A0A6J1D7Q5	4.4e-57	50.45	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]
K7NBR4	1.9e-55	47.73	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]

BLAST of Moc08g22250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	6.6e-29	32.84	ribonuclease 1	[more]
AT1G14220.1	2.3e-26	31.68	Ribonuclease T2 family protein	[more]
AT1G26820.1	7.6e-25	31.58	ribonuclease 3	[more]
AT1G14210.1	1.9e-23	29.56	Ribonuclease T2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 14..194 e-value: 2.8E-39 score: 135.2
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 12..197
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 14..205 e-value: 5.7E-50 score: 171.9
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 14..206
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 12..197
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 95..106
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 14..195 e-value: 4.46121E-55 score: 172.517

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g22250.1	Moc08g22250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding