Moc08g21760 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g21760
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	cytochrome P450 CYP72A219-like
Location	chr8: 16021930 .. 16026894 (-)
RNA-Seq Expression	Moc08g21760
Synteny	Moc08g21760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACAACAGTGTATTTCCGATTGCAGCTCGAACTCGGCCCCCGGACCGATCTGAATACTTGGGCGGACCTGCACAAAAAGGTGAGCACTCCGACGATCAAGTCAGTACAGGTCGGATTCTTAGTTTAGTTCGAGGTCAGAAATCATCGTACCTGATCGTGGAGTGAGGTCCTCTACTTATGGGTGGTAGTCCCGCTAATCTCGCGACGGTTACACCCGGTAATCTCGGGACCGTTGGTTACACCCGATAATCACGCCGCTGACAGCAGCTCACATCGGCCCCTACCGAGCTTCCCGGTAGGTCGGACCTTGGCCAGGTTCACCTCGGCCCTCATACTTAGCATCCATCAACGCTAACGGTGGTGATCTCGGCAGTCCGAGCTGGACATGACTCATAAGTCATCTTGGAGAACCAATAGGGGTCCTCCACGTGTCCAGGGTATTCTTTCCCCAAACAAAACAGTCAACAATATCTAGAGATTCACGAAAAAGGAAGAAAAAAGGAAAAAAAGATGGAGGAACAAAAATATATATATTTGGCGTGGGGAAGTGGAAAGTGGGAAATAGGGATCCATGGAGATGTGGAATATTTTGACGTTGGGTGTGGGGTTGTGCTTGGCGGTGGTGTGGGGATGGAGGGTTGTGAACTGGATTTGGGTGAGGCCCAAGCAGCTGGAGAAGAGGCTCACGCAGCAGGGCCTCGCCGGAAATTCCTACCGCCTTCTCTTTGGAGATTCCAACGATATTGCGGCAGCGATGCGGCGGGCGAGATCCGGGCCGCCCATGAGCTTCTCCCACGATATTGCTCCACGCGCCACCCCCTCCGCGCATCTCACTATCCATAAATATGGTACATCTCTTCTTTGTTCCATTAAGATTCAAACTCCTGATTTTTTAGTTTAAAATATATATAATAATTAATTGAATTAAAGTTTTGATACTGTTTAGGCTGAGTGAACAAGTACGGTTGAATATATGAATCTGATTGCATAAGGGTCTTTATAGAGTATTGAATAAAAAGGAGGGGAAAATGGATTCGTAGTCAGTCCGCCGGGTGATTATAATATTAGTTTTTTGTGAAGCTTAATAAATATCTTAAAACTACAATGATTGAAAATAAATTAAATAAAGTATCTAAAAACATAGGAACGAAATTGATAAAATAATCTCGAACTATAGTATTCAAATCAAAATTAGAGAGATTTGAAAGTAATGTTGAACTATATATTTCTTTTAGTATTACAATTTAAATAAGTGGAGATTCAAACTTGTATTTTCTTTGTGCGGGCAAAAAAAGTTTGGTTGAGATAATTTTATGAAACGTTATCGGATATAGTTCATTAAGTTGTATTCTAAATAAAAGTATATTTGTAGGTAAGGATTGTTTCACATGGTTTGGCACAACTCCAAGAGTGTATATAAGAGATCCAGAACAGCTGAAGATTGTTTTCTCTCAAATCAATGACATTCGTAAGTCATCTTCTTTCCCAGCCAATATTGTAAGGAGAATGGGAAGTGGGCTTGTCACTCTGGAGGGACCTAAATGGGCTAAACATAGAAAGATCATCAACCCTGCATTTCACATGGAGAAGTTGAAGGTAGACTTTCTAATTCCATTATTTTTTCTTTAAAGAAAGATGTTAAATTATTATAGTTTAATCTTAGTTTCAGTTGAGTCAAATCTTAATTTAAAACTTTCGACTTGAAATGTTTGCAGGATATGTTTCCAGCGTTCTCCCAGAGTTGTAGCGAGATGATTAGCAAATGGGAAACAATGGTTCTTAAAGATGGGTCTTGTGAACTAGACGTGTGGCCTGATTTACAAAATATGACCGCAGATGTGATATCTCGAACTGCATTTGGGAGTAGCTACGAGGAAGGAAAAAAGATATTCCAACTCTTGAAAGAGTGGGCTGTGCTACTAATGACACGCCTAACCAATCGAGTCTATTATATTCCGGGATGGAGGTAATTAAGATGCTGCATTTGTTGATACTTTTACCGTTGTAAGTAGAAGATACTTATCAGTAAGCACCTAAATTCTATCCAATTAAAATGATGTTTTATTTTCAAGACGCTTGTTCATCTTTCTCTCTTTCTTTTTTCATTTCCTACATTAAAAAAAAAAAACACTAATTATATATTTCGAAATGTTGTCCTAGCTTTTTACCAACCAAGTTAAACAAGAGGACGGAAGAGATTGATAAGAAAATTCGAGACATGGTTTTGGGTATTATAAGTAAACGACAAAGTGCTATGAAGGCAGATGAAGCTTCCAATGATGATCTGCTAGGCATCCTTTTAGAATCAAATGCAAGTCAAATTCAGGAAAATGGAAACAGGAAAGATGTTGGAATGAGTATCCAAGATGTTATCAGTGAATGCAGGCTTTTCTATTTTGCTGGCCAAGAAACCACAGCAGCATTGCTTGTTTGGACAATGGTCTTACTTGGTCATCACTCGGAATGGCAAGACCGAGCAAGAGCAGAAGTCCTAGAAGTCTTCAGCGATAACAACAAATTGGATTTTGATGGTTTAAGTCGCCTTAAAATTGTAAGTAATTATGATTTTTTATTTTTTTTTTTTCTATTTTCTCACACGATGAATCACCAATCAACTCATAAAAGGTAAGCTCATAGGTTACAGTAAATTTAATCTTTTATAGATATTTTAATACACTTCCCCTCATATATTAAATTGGGAAAAAATTACATTACAGGAATTTGATTACAAGTTATACCGATATGAGTTAGTTATACCGATATGAGTTAGGCTAAATTTAGAGCCGGATTAGTATTCAATACAAATTTATTTTCAATATTTGTTTGGTATTTTTTGTTTTTTAATGTTCAATAATATTTTAAATTTTAATCCAAATTTGGTTTCAATATTTGTTTGGTATTTTTGTTTTTTAATGTTCAATAATATTTTAAATTGGACTAAATATTTAGTTGTTAGTGATCACTAGTTAACAAATATTATTGCTAAACACCCCTCTGTTTTTCATTGAATTGGTTTTAGATTTTTGTTTTTTTATCACCCACTAAATGCACCATTAATCTTTTATAGATACCTTAATAAAAAATAGTATTATTACATTGATAACAGGTAACTATGATATTGAACGAGGTTCTCCGACTATACCCTCCAGTTGGTATGCTAGCTAGAGAGATTCACACGGAAACAAAATTGGGAAATCTGACATTACCCGTTGGAGCATCAATAGGCATACCAATTGTATACATTCACCAAGATCCCAAAATATGGGGTGAAGATGCAGGTGAGTTCAAACCAGAGAGGTTTTCAGAGGGAATTTCCAAAGCAACAAAGAATCAAGTATGCTTCATCCCTTTTGGATGGGGTCCTCGAATCTGTCTCGGCCAAAACTTTGCTATGATAGAAGCAAAAATAGCGTTATTGATGATTTTGCGACGATTCTCTTTTGAACTTTCTCCAAGTTATACTCATGCTCCTGTATCAAACATCACTATTCAACCACAACATGGGGCTCATCTCATTCTTCACAGGCTATCATAGTTTTCCAAAGGGTTGTAAGTTGTAACAATTATCTTTAGCTTTTTTCCTGTTGTGTAAACATGACCATAAAGAAAGACTTATAATTTCACTAGTAATTCTCCATTACAAATTGGAATAATGTGATTTAGAAAGCTCCAGTTATAATATTGCAAATACAAGTTAATTAGTTGTACGTGTTTGTTATTGTTAGAACTTCAATGAATGATGTTGGCATTTTTAACAGATAGTTCTACTGCATTTTGAGCATCAAACAAGTAATTTCAAGTAAGGAAATTGAGGATTTCTAAGGAAATTGAGGATTTTCCCCCCTTTTTTAGCTCAATAACAAGGATGCCATATTTCGTGACGCGAGAAAAATGTCTCTACATGTAAAAAATTAGCAATGCACATAAAAAAAATTCACTTAAAAGTAACTCTCCTTAGGTTATTTAAGTACTATCGGGTATTCCAATTTACACTAACAGCACAAACATATTTAGAAAAATAAAGAATAATACATAATTAATTAAGCTTCATCCATAAATTCAACAAAATAAACGAGGAAGAGAGAAGAAACAAAACACTGATTTATAGTAATTAAATGTCTATGTTAAAAGTTAATTTGTAATTTTCAAGATAAGTTTCAGTAAGTTGAATTGATAAGTCGTTTGATTTCTTCTAAGTGACTTCTTGGAATAAGAAATGTTTGTCTAAATTACTATAGCAATTACCTAGGACTTTGAATAGGGGTTTCTAGAGCTATTCCTAGCACCTATAAATAGATGTACTCTCCATCATTTGTAAGCAGCACAAGCAATCGAGCTCTCCAATTGAGTAAAGTGCCAAAGCACCCATAGAAGAAGTTTCTCTCCATTATAAGTTCTCTTCTATTTTATTTTAGAGCCATCCTAAAATTAAACATGATTTTTAGTTGGGTTCCAATTTTGTTTATTCTCCCAACGAACCAAGTTATGGTATTTGATGGAGAGCACTATGAATATTGACAAAGTCAAATGGAGACAATTTTCTTCTCAAGATTTTTGATAAATTATTGAAAGAAGACTACGAAGATAAAGGTGCCTGGGAGATGATATAAGAACAAGAGGAGTTGAAAAAGAATAAAAGCTAAAATGCTTTAGCTCTTCGTCTCATTCAACAAGGTGTTAGCAAGTCCATTTTTCCTTGGAATTTTTGTATTAAAAAAGTGAGATGCTTGGAAAATTTGCAAAATGAATTTCAGGTGTTTATGCAAGGATATGCACAACAGTGTATTTTAGATTGCAGCTCGAACTCGGCCTCCGGACCGATCTGAATACTTGGGCGGACCTGCACAAAAAGGTGAGCACTTCGACGATCAAGTCAGTATAGGTCGGATTCCCAGTTTAGTTCGAGGTCAGAAAATCATTGTACCTGATCGTGGAGTGAGGTCCTCTACTTATGGGTGGTAG

mRNA sequence

ATGCACAACAGTGTATTTCCGATTGCAGCTCGAACTCGGCCCCCGGACCGATCTGAATACTTGGGCGGACCTGCACAAAAAGGTGAGCACTCCGACGATCAAGTCAGTACAGGTCGGATTCTTAGTTTAGTTCGAGGTCAGAAATCATCGTACCTGATCGTGGAGCCCAAGCAGCTGGAGAAGAGGCTCACGCAGCAGGGCCTCGCCGGAAATTCCTACCGCCTTCTCTTTGGAGATTCCAACGATATTGCGGCAGCGATGCGGCGGGCGAGATCCGGGCCGCCCATGAGCTTCTCCCACGATATTGCTCCACGCGCCACCCCCTCCGCGCATCTCACTATCCATAAATATGGTAAGGATTGTTTCACATGGTTTGGCACAACTCCAAGAGTGTATATAAGAGATCCAGAACAGCTGAAGATTGTTTTCTCTCAAATCAATGACATTCGTAAGTCATCTTCTTTCCCAGCCAATATTGTAAGGAGAATGGGAAGTGGGCTTGTCACTCTGGAGGGACCTAAATGGGCTAAACATAGAAAGATCATCAACCCTGCATTTCACATGGAGAAGTTGAAGGATATGTTTCCAGCGTTCTCCCAGAGTTGTAGCGAGATGATTAGCAAATGGGAAACAATGGTTCTTAAAGATGGGTCTTGTGAACTAGACGTGTGGCCTGATTTACAAAATATGACCGCAGATGTGATATCTCGAACTGCATTTGGGAGTAGCTACGAGGAAGGAAAAAAGATATTCCAACTCTTGAAAGAGTGGGCTGTGCTACTAATGACACGCCTAACCAATCGAGTCTATTATATTCCGGGATGGAGCTTTTTACCAACCAAGTTAAACAAGAGGACGGAAGAGATTGATAAGAAAATTCGAGACATGGTTTTGGGTATTATAAGTAAACGACAAAGTGCTATGAAGGCAGATGAAGCTTCCAATGATGATCTGCTAGGCATCCTTTTAGAATCAAATGCAAGTCAAATTCAGGAAAATGGAAACAGGAAAGATGTTGGAATGAGTATCCAAGATGTTATCAGTGAATGCAGGCTTTTCTATTTTGCTGGCCAAGAAACCACAGCAGCATTGCTTGTTTGGACAATGGTCTTACTTGGTCATCACTCGGAATGGCAAGACCGAGCAAGAGCAGAAGTCCTAGAAGTCTTCAGCGATAACAACAAATTGGATTTTGATGGTTTAAGTCGCCTTAAAATTGTAACTATGATATTGAACGAGGTTCTCCGACTATACCCTCCAGTTGGTATGCTAGCTAGAGAGATTCACACGGAAACAAAATTGGGAAATCTGACATTACCCGTTGGAGCATCAATAGGCATACCAATTGTATACATTCACCAAGATCCCAAAATATGGGGTGAAGATGCAGGTGAGTTCAAACCAGAGAGGTTTTCAGAGGGAATTTCCAAAGCAACAAAGAATCAAATTGCAGCTCGAACTCGGCCTCCGGACCGATCTGAATACTTGGGCGGACCTGCACAAAAAGGTGAGCACTTCGACGATCAAGTCAGTATAGGTCGGATTCCCAGTTTAGTTCGAGGTCAGAAAATCATTGTACCTGATCGTGGAGTGAGGTCCTCTACTTATGGGTGGTAG

Coding sequence (CDS)

ATGCACAACAGTGTATTTCCGATTGCAGCTCGAACTCGGCCCCCGGACCGATCTGAATACTTGGGCGGACCTGCACAAAAAGGTGAGCACTCCGACGATCAAGTCAGTACAGGTCGGATTCTTAGTTTAGTTCGAGGTCAGAAATCATCGTACCTGATCGTGGAGCCCAAGCAGCTGGAGAAGAGGCTCACGCAGCAGGGCCTCGCCGGAAATTCCTACCGCCTTCTCTTTGGAGATTCCAACGATATTGCGGCAGCGATGCGGCGGGCGAGATCCGGGCCGCCCATGAGCTTCTCCCACGATATTGCTCCACGCGCCACCCCCTCCGCGCATCTCACTATCCATAAATATGGTAAGGATTGTTTCACATGGTTTGGCACAACTCCAAGAGTGTATATAAGAGATCCAGAACAGCTGAAGATTGTTTTCTCTCAAATCAATGACATTCGTAAGTCATCTTCTTTCCCAGCCAATATTGTAAGGAGAATGGGAAGTGGGCTTGTCACTCTGGAGGGACCTAAATGGGCTAAACATAGAAAGATCATCAACCCTGCATTTCACATGGAGAAGTTGAAGGATATGTTTCCAGCGTTCTCCCAGAGTTGTAGCGAGATGATTAGCAAATGGGAAACAATGGTTCTTAAAGATGGGTCTTGTGAACTAGACGTGTGGCCTGATTTACAAAATATGACCGCAGATGTGATATCTCGAACTGCATTTGGGAGTAGCTACGAGGAAGGAAAAAAGATATTCCAACTCTTGAAAGAGTGGGCTGTGCTACTAATGACACGCCTAACCAATCGAGTCTATTATATTCCGGGATGGAGCTTTTTACCAACCAAGTTAAACAAGAGGACGGAAGAGATTGATAAGAAAATTCGAGACATGGTTTTGGGTATTATAAGTAAACGACAAAGTGCTATGAAGGCAGATGAAGCTTCCAATGATGATCTGCTAGGCATCCTTTTAGAATCAAATGCAAGTCAAATTCAGGAAAATGGAAACAGGAAAGATGTTGGAATGAGTATCCAAGATGTTATCAGTGAATGCAGGCTTTTCTATTTTGCTGGCCAAGAAACCACAGCAGCATTGCTTGTTTGGACAATGGTCTTACTTGGTCATCACTCGGAATGGCAAGACCGAGCAAGAGCAGAAGTCCTAGAAGTCTTCAGCGATAACAACAAATTGGATTTTGATGGTTTAAGTCGCCTTAAAATTGTAACTATGATATTGAACGAGGTTCTCCGACTATACCCTCCAGTTGGTATGCTAGCTAGAGAGATTCACACGGAAACAAAATTGGGAAATCTGACATTACCCGTTGGAGCATCAATAGGCATACCAATTGTATACATTCACCAAGATCCCAAAATATGGGGTGAAGATGCAGGTGAGTTCAAACCAGAGAGGTTTTCAGAGGGAATTTCCAAAGCAACAAAGAATCAAATTGCAGCTCGAACTCGGCCTCCGGACCGATCTGAATACTTGGGCGGACCTGCACAAAAAGGTGAGCACTTCGACGATCAAGTCAGTATAGGTCGGATTCCCAGTTTAGTTCGAGGTCAGAAAATCATTGTACCTGATCGTGGAGTGAGGTCCTCTACTTATGGGTGGTAG

Protein sequence

MHNSVFPIAARTRPPDRSEYLGGPAQKGEHSDDQVSTGRILSLVRGQKSSYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVTLEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPERFSEGISKATKNQIAARTRPPDRSEYLGGPAQKGEHFDDQVSIGRIPSLVRGQKIIVPDRGVRSSTYGW

Homology

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BLAST of Moc08g21760 vs. NCBI nr
Match: XP_022158406.1 (11-oxo-beta-amyrin 30-oxidase-like [Momordica charantia])

HSP 1 Score: 869.8 bits (2246), Expect = 1.2e-248
Identity = 431/450 (95.78%), Postives = 439/450 (97.56%), Query Frame = 0


Query: 35  VSTGRILSLVRGQK-SSYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSG 94
           +  G  L++V G +  +++ V PKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSG
Sbjct: 9   LGVGLCLAVVWGWRVVNWIWVRPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSG 68

Query: 95  PPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSS 154
           PPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSS
Sbjct: 69  PPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSS 128

Query: 155 SFPANIVRRMGSGLVTLEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMV 214
           SFPANIVRRMGSGLVTLEGPKW KHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMV
Sbjct: 129 SFPANIVRRMGSGLVTLEGPKWXKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMV 188

Query: 215 LKDGSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIP 274
           LKDGSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIP
Sbjct: 189 LKDGSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIP 248

Query: 275 GWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQEN 334
           GWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQEN
Sbjct: 249 GWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQEN 308

Query: 335 GNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDN 394
           GNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDN
Sbjct: 309 GNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDN 368

Query: 395 NKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIH 454
           NKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIH
Sbjct: 369 NKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIH 428

Query: 455 QDPKIWGEDAGEFKPERFSEGISKATKNQI 484
           QDPKIWGEDAGEFKPERFSEGISKATKNQ+
Sbjct: 429 QDPKIWGEDAGEFKPERFSEGISKATKNQV 458

[Close]

BLAST of Moc08g21760 vs. NCBI nr
Match: XP_023006210.1 (cytochrome P450 CYP72A219-like [Cucurbita maxima])

HSP 1 Score: 695.3 bits (1793), Expect = 4.2e-196
Identity = 342/434 (78.80%), Postives = 384/434 (88.48%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ V PK+LEK L QQGLAGNSYRLLFGDS D AAA+R+AR+  P+SFSH IA RATPS
Sbjct: 22  NWMWVTPKRLEKCLRQQGLAGNSYRLLFGDSKDTAAAVRQART-QPISFSHRIAARATPS 81

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           +H+TI +YGKD FTW G +PRVYI  PEQ+K+VFSQINDIRK++SFP    RR+G G+V+
Sbjct: 82  SHITIDQYGKDSFTWIGPSPRVYITQPEQVKMVFSQINDIRKATSFPFR--RRIGGGVVS 141

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLKDMFPAFS+SCSEMI+KWE M+ K+G CELDVWPDLQN
Sbjct: 142 LEGAKWAKHRKIINPAFHMEKLKDMFPAFSKSCSEMINKWEMMITKEGCCELDVWPDLQN 201

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           M ADVISRTAFGSSYEEGKKIFQLLK+WA LLM  +   VY++PG  FLPTKLNKR +EI
Sbjct: 202 MAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFLPGLRFLPTKLNKRMDEI 261

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           + KIRDM+ GIISKRQ+AMK  EASNDDLLGILLESN+ +IQE+ N+KDVGMSI++VI+E
Sbjct: 262 NGKIRDMIWGIISKRQNAMKKGEASNDDLLGILLESNSREIQEHKNKKDVGMSIEEVIAE 321

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLFYFAGQETTAALL WTMVLLG +SEWQDRARAEVLEVF DN KLDFDGLSRLK+VTM
Sbjct: 322 CRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNKKLDFDGLSRLKVVTM 381

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           ILNEVLRLYPPVGMLAREIH ETKLGNLTLPVG SIGIPIV IHQDP IWGEDA EFKPE
Sbjct: 382 ILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTIWGEDASEFKPE 441

Query: 470 RFSEGISKATKNQI 484
           RF+EGISKATKNQ+
Sbjct: 442 RFAEGISKATKNQV 452

[Close]

BLAST of Moc08g21760 vs. NCBI nr
Match: XP_023529493.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.2 bits (1785), Expect = 3.6e-195
Identity = 341/434 (78.57%), Postives = 381/434 (87.79%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ + PK+LEK L  QGLAGNSYRLLFGDS D AAA+R+A++  P+SFSH IA RATPS
Sbjct: 22  NWMWLRPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKT-QPISFSHRIAARATPS 81

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           +H+TI +YGKD FTW G TPRVYI  PEQ+K+VFSQINDIRK++SFP    RRMG G+V+
Sbjct: 82  SHITIDQYGKDSFTWIGPTPRVYITHPEQVKMVFSQINDIRKTTSFPFR--RRMGGGVVS 141

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMI+KWE M+ K+G CELDVWPDLQN
Sbjct: 142 LEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKEGCCELDVWPDLQN 201

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           M ADVISRTAFGSSYEEGKKIFQLLK+WA LLM  +   VY+IPG  FLPTKLNKR +EI
Sbjct: 202 MAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLRFLPTKLNKRMDEI 261

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           +  IRDM+ GII+KRQ+AMK  EASNDDLLGILLESN+ +IQE+ N+KDVGMSI++VI+E
Sbjct: 262 NGNIRDMIWGIINKRQNAMKKGEASNDDLLGILLESNSREIQEHKNKKDVGMSIEEVIAE 321

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLFYFAGQETTAALL WTMVLLG +SEWQDRARAEVLEVF DN K DFDGLSRLKIVTM
Sbjct: 322 CRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNKKFDFDGLSRLKIVTM 381

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           ILNEVLRLYPPVGMLAREIH ETKLGNLTLPVG SIGIPIV IHQDP IWGEDA EFKPE
Sbjct: 382 ILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTIWGEDASEFKPE 441

Query: 470 RFSEGISKATKNQI 484
           RF+EGISKATKNQ+
Sbjct: 442 RFAEGISKATKNQV 452

[Close]

BLAST of Moc08g21760 vs. NCBI nr
Match: XP_022924709.1 (cytochrome P450 CYP72A219-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 688.3 bits (1775), Expect = 5.2e-194
Identity = 336/434 (77.42%), Postives = 380/434 (87.56%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ + PK+LEK L  QGLAGNSYRLLFGDS D AAA+R+A++  P+SFSH IA RATPS
Sbjct: 44  NWMWLTPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKT-QPISFSHRIAARATPS 103

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           +H+TIH+YGKD FTW G TPRVYI  PEQ+K+VFSQINDIRK++ FP    RRMG G+V+
Sbjct: 104 SHITIHRYGKDSFTWIGPTPRVYITHPEQVKVVFSQINDIRKATLFPFR--RRMGGGVVS 163

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMI+KWE M+ K+G CELDVWPDLQN
Sbjct: 164 LEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKEGCCELDVWPDLQN 223

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           M ADVISRTAFGSSYEEGKKIFQLLK+WA LLM  +   VY+IPG  FLPTKLNKR +EI
Sbjct: 224 MAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLRFLPTKLNKRMDEI 283

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           +  IRDM+ GII+KRQ+AMK  EASNDDLLGILLESN+ +IQE+ N+KDVGMS+++VI+E
Sbjct: 284 NGNIRDMIWGIINKRQNAMKKGEASNDDLLGILLESNSREIQEHKNKKDVGMSMEEVIAE 343

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLFYFAGQETTAALL WTMVLLG +SEWQDRARAEVLE+F DN K DFDGLSRLKIVTM
Sbjct: 344 CRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNKKFDFDGLSRLKIVTM 403

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           ILNEVLRLYPPVG+L+REIH ETKLGNLTLPVG SIGIPIV IHQDP +WGEDA EFKPE
Sbjct: 404 ILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTLWGEDASEFKPE 463

Query: 470 RFSEGISKATKNQI 484
           RF EGISKATKNQ+
Sbjct: 464 RFVEGISKATKNQV 474

[Close]

BLAST of Moc08g21760 vs. NCBI nr
Match: XP_022924700.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 688.3 bits (1775), Expect = 5.2e-194
Identity = 336/434 (77.42%), Postives = 380/434 (87.56%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ + PK+LEK L  QGLAGNSYRLLFGDS D AAA+R+A++  P+SFSH IA RATPS
Sbjct: 44  NWMWLTPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKT-QPISFSHRIAARATPS 103

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           +H+TIH+YGKD FTW G TPRVYI  PEQ+K+VFSQINDIRK++ FP    RRMG G+V+
Sbjct: 104 SHITIHRYGKDSFTWIGPTPRVYITHPEQVKVVFSQINDIRKATLFPFR--RRMGGGVVS 163

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMI+KWE M+ K+G CELDVWPDLQN
Sbjct: 164 LEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKEGCCELDVWPDLQN 223

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           M ADVISRTAFGSSYEEGKKIFQLLK+WA LLM  +   VY+IPG  FLPTKLNKR +EI
Sbjct: 224 MAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLRFLPTKLNKRMDEI 283

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           +  IRDM+ GII+KRQ+AMK  EASNDDLLGILLESN+ +IQE+ N+KDVGMS+++VI+E
Sbjct: 284 NGNIRDMIWGIINKRQNAMKKGEASNDDLLGILLESNSREIQEHKNKKDVGMSMEEVIAE 343

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLFYFAGQETTAALL WTMVLLG +SEWQDRARAEVLE+F DN K DFDGLSRLKIVTM
Sbjct: 344 CRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNKKFDFDGLSRLKIVTM 403

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           ILNEVLRLYPPVG+L+REIH ETKLGNLTLPVG SIGIPIV IHQDP +WGEDA EFKPE
Sbjct: 404 ILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTLWGEDASEFKPE 463

Query: 470 RFSEGISKATKNQI 484
           RF EGISKATKNQ+
Sbjct: 464 RFVEGISKATKNQV 474

[Close]

BLAST of Moc08g21760 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.2e-130
Identity = 237/434 (54.61%), Postives = 300/434 (69.12%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ V P++LE+ L +QG  GNSYRL +GD  + +   R+A+   P++ S D   R  P 
Sbjct: 30  NWVWVSPRKLEESLRKQGFRGNSYRLFYGDLKESSEMTRKAKL-KPINLSDDPVLRVRPF 89

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
            H T+ KYGK  F W G TPRV I DPE +K +  +     K    P  +V+    GL  
Sbjct: 90  IHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMVKSYKFNKPKRNP--LVKLFADGLAN 149

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
            EG  WAKHRK++NPAFH+E+LK M PA   SC EM+SKW+ M+ KDGS ELDVWP LQ 
Sbjct: 150 HEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVSKWDKMISKDGSRELDVWPFLQR 209

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           +T+DVIS TAFGSSYEEG  +F+L  E A L+M  L +   YIPGWS+LPTK N++ +EI
Sbjct: 210 LTSDVISHTAFGSSYEEGNIVFELQTEQAELVMKTLQS--VYIPGWSYLPTKRNRKMKEI 269

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           D+K +  ++ II+K+  AM+A E S DD+LGILLESN  + Q    +K+VGMSIQ+V+ E
Sbjct: 270 DRKTQSCLMNIINKKTKAMQAGEGSTDDILGILLESNLKE-QLGQGKKNVGMSIQEVMGE 329

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           C+ FYFAGQETT+ LLVWTMVLL  H  WQ RAR EVL+ F  N K DFD L+ LKIVTM
Sbjct: 330 CKQFYFAGQETTSGLLVWTMVLLSIHPNWQARAREEVLQQFG-NAKPDFDNLNHLKIVTM 389

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           IL EVLRLYPPV  L R +  ET LG++TLP G  I +PI+ +H D  IWG+DA EF PE
Sbjct: 390 ILYEVLRLYPPVDTLFRRVDQETTLGDITLPAGVQISLPIMILHHDQNIWGDDAKEFNPE 449

Query: 470 RFSEGISKATKNQI 484
           RFSEG+SKATKNQ+
Sbjct: 450 RFSEGVSKATKNQV 456

[Close]

BLAST of Moc08g21760 vs. ExPASy Swiss-Prot
Match: H1A988 (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 5.5e-130
Identity = 241/434 (55.53%), Postives = 299/434 (68.89%), Query Frame = 0


Query: 52  LIVEPKQLEKRLTQQGLAGNSYRLLFGDSND--IAAAMRRARSGPPMSFSHDIAPRATPS 111
           L + PK+LE+ L  QGL G+ Y+L   +S    +    + A+S        D APR    
Sbjct: 32  LWLRPKRLERHLRAQGLHGDPYKLSLDNSKQTYMLKLQQEAQSKSIGLSKDDAAPRIFSL 91

Query: 112 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 171
           AH T+HKYGK+ F W GT P+V I DPEQ+K VF++I D  K    P  I + +  GLV 
Sbjct: 92  AHQTVHKYGKNSFAWEGTAPKVIITDPEQIKEVFNKIQDFPKPKLNP--IAKYISIGLVQ 151

Query: 172 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 231
            EG KWAKHRKIINPAFH+EKLK M PAFS SC EMISKW+ ++  DG+CE+DVWP LQN
Sbjct: 152 YEGDKWAKHRKIINPAFHLEKLKGMLPAFSHSCHEMISKWKGLLSSDGTCEVDVWPFLQN 211

Query: 232 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 291
           +T DVISRTAFGSSY EG KIF+LLK     LMT    R   IP W  LP+   +R +EI
Sbjct: 212 LTCDVISRTAFGSSYAEGAKIFELLKRQGYALMTA---RYARIPLWWLLPSTTKRRMKEI 271

Query: 292 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 351
           ++ IRD + GII KR+ A+K+ ++++DDLLGILL+SN  + + + N K  GM+ Q+V+ E
Sbjct: 272 ERGIRDSLEGIIRKREKALKSGKSTDDDLLGILLQSNHIENKGDENSKSAGMTTQEVMEE 331

Query: 352 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 411
           C+LFY AGQETTAALL WTMVLLG H EWQ RAR EVL+VF + N  +F+GL RLKIVTM
Sbjct: 332 CKLFYLAGQETTAALLAWTMVLLGKHPEWQARARQEVLQVFGNQNP-NFEGLGRLKIVTM 391

Query: 412 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 471
           IL EVLRLYPP   L R +  + KLGNL LP G  + +PI+ IH D  IWG DA EF PE
Sbjct: 392 ILYEVLRLYPPGIYLTRALRKDLKLGNLLLPAGVQVSVPILLIHHDEGIWGNDAKEFNPE 451

Query: 472 RFSEGISKATKNQI 484
           RF+EGI+KATK Q+
Sbjct: 452 RFAEGIAKATKGQV 459

[Close]

BLAST of Moc08g21760 vs. ExPASy Swiss-Prot
Match: Q2MJ19 (Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.9e-128
Identity = 226/435 (51.95%), Postives = 314/435 (72.18%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ ++PK++EK L +QGL GN YRLL GD+ D     ++ +S  PM+ S DIAPR  P 
Sbjct: 28  NWMWLKPKKIEKLLREQGLQGNPYRLLLGDAKDYFVMQKKVQS-KPMNLSDDIAPRVAPY 87

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
            H  +  +GK  F WFG  P V + +PEQ++ VF+++++  K       ++ R   GLV 
Sbjct: 88  IHHAVQTHGKKSFIWFGMKPWVILNEPEQIREVFNKMSEFPKVQYKFMKLITR---GLVK 147

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KW+KHR+IINPAFHMEKLK M P F +SC+++IS WE M+  +GSCE+DVWP LQ+
Sbjct: 148 LEGEKWSKHRRIINPAFHMEKLKIMTPTFLKSCNDLISNWEKMLSSNGSCEMDVWPSLQS 207

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           +T+DVI+R++FGSSYEEG+K+FQL  E   L+M  L   +  IP W FLPT  +++  E 
Sbjct: 208 LTSDVIARSSFGSSYEEGRKVFQLQIEQGELIMKNLMKSL--IPLWRFLPTADHRKINEN 267

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           +K+I   +  II+KR+ A+KA EA+ +DLLG+LLESN  +I+E+GN K++G+S+++V+ E
Sbjct: 268 EKQIETTLKNIINKREKAIKAGEATENDLLGLLLESNHREIKEHGNVKNMGLSLEEVVGE 327

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLF+ AGQETT+ LLVWTMVLL  + +WQ+RAR EVLE+F  N K DFDGL++LKI+ M
Sbjct: 328 CRLFHVAGQETTSDLLVWTMVLLSRYPDWQERARKEVLEIFG-NEKPDFDGLNKLKIMAM 387

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           IL EVLRLYPPV  +AR++  + KLG+LTL  G  + +PIV IH D ++WG+DA  F PE
Sbjct: 388 ILYEVLRLYPPVTGVARKVENDIKLGDLTLYAGMEVYMPIVLIHHDCELWGDDAKIFNPE 447

Query: 470 RFSEGISKATKNQIA 485
           RFS GISKAT  + +
Sbjct: 448 RFSGGISKATNGRFS 455

[Close]

BLAST of Moc08g21760 vs. ExPASy Swiss-Prot
Match: H1A981 (11-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula OX=3880 GN=CYP72A63 PE=1 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.3e-127
Identity = 235/449 (52.34%), Postives = 305/449 (67.93%), Query Frame = 0


Query: 40  ILSLVRGQKSSYLIVEPKQLEKRLTQQGLAGNSYRLLF---GDSNDIAAAMRRARSGPPM 99
           +L+++     + L ++PK+ EK L  QG  G  Y L         +    +++      +
Sbjct: 20  LLAVIPWYLLNKLWLKPKRFEKLLKAQGFQGEPYNLSVLKDKSKQNYMLKLQQEDKSKSI 79

Query: 100 SFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFP 159
             S + AP      H T+ KYG + F W GTTPRV I DP+Q+K VF++I+D  K     
Sbjct: 80  GLSKEAAPSIFTPVHQTVRKYGNNSFLWEGTTPRVIITDPDQIKDVFNKIDDFPKPKL-- 139

Query: 160 ANIVRRMGSGLVTLEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKD 219
            +I + +  G++  EG KWAKHRKI NPAFH+EKLK M PAFS SC+EMISKW+ ++  D
Sbjct: 140 RSIAKYLSVGILDHEGKKWAKHRKIANPAFHLEKLKVMLPAFSHSCNEMISKWKELLSSD 199

Query: 220 GSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMT-RLTNRVYYIPGW 279
           G+CE+DVWP LQN T DVISRTAFGSSY EG K+FQLLK+   LLMT R TN     P W
Sbjct: 200 GTCEIDVWPSLQNFTCDVISRTAFGSSYAEGTKLFQLLKKQGFLLMTGRHTNN----PLW 259

Query: 280 SFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGN 339
             L T    + +EID++I D + GII KR+ A+K  E +NDDLLGILL+SN ++ Q  GN
Sbjct: 260 GLLATTTKTKMKEIDREIHDSLEGIIEKREKALKNGETTNDDLLGILLQSNHAEKQGQGN 319

Query: 340 RKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNK 399
            K++GM+ QDVI EC+LFY AGQETT++LLVWTMVLLG + EWQ RAR EVL+VF + N 
Sbjct: 320 SKNIGMTTQDVIDECKLFYLAGQETTSSLLVWTMVLLGRYPEWQARAREEVLQVFGNQNP 379

Query: 400 LDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQD 459
            + +GLS+LKIVTMIL EVLRL+PP+    R +  + KLGNL LP G  I +PI+ IHQD
Sbjct: 380 -NNEGLSQLKIVTMILYEVLRLFPPLIYFNRALRKDLKLGNLLLPEGTQISLPILLIHQD 439

Query: 460 PKIWGEDAGEFKPERFSEGISKATKNQIA 485
             +WG+DA EFKPERF+EGI+KATK Q++
Sbjct: 440 HDLWGDDAKEFKPERFAEGIAKATKGQVS 461

[Close]

BLAST of Moc08g21760 vs. ExPASy Swiss-Prot
Match: W8JWW3 (Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 8.2e-126
Identity = 234/434 (53.92%), Postives = 308/434 (70.97%), Query Frame = 0


Query: 54  VEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPSAHLT 113
           ++PK+LEKRL Q GL GNSYRLL GD  + +   + A+S  P++F++ I PR  P  H  
Sbjct: 46  IKPKKLEKRLRQLGLKGNSYRLLIGDLGETSQMDKEAKS-KPINFTNHIIPRIMPFLHKI 105

Query: 114 IHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVTLEGP 173
           I  +GK+ F W G  P V I DPE +K V ++    +K  S   ++ + +  G+   +  
Sbjct: 106 IQSHGKNPFIWQGPNPAVIITDPELIKEVLTKNYTYQKPPS--DDLGKLISQGVAAYDKD 165

Query: 174 KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETM--VLKDGSCELDVWPDLQNMT 233
           KWAKHR+++NPAFH+EKLK M PAF  SCSEM+ KWE +     +GS ELDVWP LQ+MT
Sbjct: 166 KWAKHRRLLNPAFHIEKLKHMLPAFHLSCSEMLKKWEKLASASAEGSFELDVWPYLQDMT 225

Query: 234 ADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEIDK 293
           +DVISRTAFGS+ EEGK IF+L KE    ++  L +  +Y PG  +LPTK  +R ++I K
Sbjct: 226 SDVISRTAFGSNLEEGKNIFKLQKEILEHILQILQS--FYFPGMLYLPTKRIRRMKKISK 285

Query: 294 KIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQ-ENGN--RKDVGMSIQDVIS 353
           ++   VL II KR  AMKA E SNDDLLGILL+SN  +I+ +NGN   K+ GMS ++VI 
Sbjct: 286 EVHSSVLNIIMKRVEAMKAGEPSNDDLLGILLDSNFKEIENDNGNNSNKNCGMSFEEVIE 345

Query: 354 ECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVT 413
           EC+LFYFAGQETTA LLVWT++LL  H EWQ RAR EVL++F  N++ DFD L+ LK++T
Sbjct: 346 ECKLFYFAGQETTAVLLVWTLLLLSKHPEWQMRARDEVLQIFG-NSEPDFDALNHLKVIT 405

Query: 414 MILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKP 473
           M++NEVLRLYPP  M++R IH +TKLG+L+LP G SI +P + +H D ++WG+DA EFKP
Sbjct: 406 MVINEVLRLYPPGFMMSRVIHEDTKLGSLSLPGGISIFLPTIMLHHDEELWGDDAKEFKP 465

Query: 474 ERFSEGISKATKNQ 483
           ERFSEGISKATK Q
Sbjct: 466 ERFSEGISKATKGQ 473

[Close]

BLAST of Moc08g21760 vs. ExPASy TrEMBL
Match: A0A6J1E0T8 (11-oxo-beta-amyrin 30-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111024896 PE=3 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 6.0e-249
Identity = 431/450 (95.78%), Postives = 439/450 (97.56%), Query Frame = 0


Query: 35  VSTGRILSLVRGQK-SSYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSG 94
           +  G  L++V G +  +++ V PKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSG
Sbjct: 9   LGVGLCLAVVWGWRVVNWIWVRPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSG 68

Query: 95  PPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSS 154
           PPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSS
Sbjct: 69  PPMSFSHDIAPRATPSAHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSS 128

Query: 155 SFPANIVRRMGSGLVTLEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMV 214
           SFPANIVRRMGSGLVTLEGPKW KHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMV
Sbjct: 129 SFPANIVRRMGSGLVTLEGPKWXKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMV 188

Query: 215 LKDGSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIP 274
           LKDGSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIP
Sbjct: 189 LKDGSCELDVWPDLQNMTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIP 248

Query: 275 GWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQEN 334
           GWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQEN
Sbjct: 249 GWSFLPTKLNKRTEEIDKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQEN 308

Query: 335 GNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDN 394
           GNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDN
Sbjct: 309 GNRKDVGMSIQDVISECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDN 368

Query: 395 NKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIH 454
           NKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIH
Sbjct: 369 NKLDFDGLSRLKIVTMILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIH 428

Query: 455 QDPKIWGEDAGEFKPERFSEGISKATKNQI 484
           QDPKIWGEDAGEFKPERFSEGISKATKNQ+
Sbjct: 429 QDPKIWGEDAGEFKPERFSEGISKATKNQV 458

[Close]

BLAST of Moc08g21760 vs. ExPASy TrEMBL
Match: A0A6J1KZI7 (cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111499007 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 2.0e-196
Identity = 342/434 (78.80%), Postives = 384/434 (88.48%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ V PK+LEK L QQGLAGNSYRLLFGDS D AAA+R+AR+  P+SFSH IA RATPS
Sbjct: 22  NWMWVTPKRLEKCLRQQGLAGNSYRLLFGDSKDTAAAVRQART-QPISFSHRIAARATPS 81

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           +H+TI +YGKD FTW G +PRVYI  PEQ+K+VFSQINDIRK++SFP    RR+G G+V+
Sbjct: 82  SHITIDQYGKDSFTWIGPSPRVYITQPEQVKMVFSQINDIRKATSFPFR--RRIGGGVVS 141

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLKDMFPAFS+SCSEMI+KWE M+ K+G CELDVWPDLQN
Sbjct: 142 LEGAKWAKHRKIINPAFHMEKLKDMFPAFSKSCSEMINKWEMMITKEGCCELDVWPDLQN 201

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           M ADVISRTAFGSSYEEGKKIFQLLK+WA LLM  +   VY++PG  FLPTKLNKR +EI
Sbjct: 202 MAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFLPGLRFLPTKLNKRMDEI 261

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           + KIRDM+ GIISKRQ+AMK  EASNDDLLGILLESN+ +IQE+ N+KDVGMSI++VI+E
Sbjct: 262 NGKIRDMIWGIISKRQNAMKKGEASNDDLLGILLESNSREIQEHKNKKDVGMSIEEVIAE 321

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLFYFAGQETTAALL WTMVLLG +SEWQDRARAEVLEVF DN KLDFDGLSRLK+VTM
Sbjct: 322 CRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEVFGDNKKLDFDGLSRLKVVTM 381

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           ILNEVLRLYPPVGMLAREIH ETKLGNLTLPVG SIGIPIV IHQDP IWGEDA EFKPE
Sbjct: 382 ILNEVLRLYPPVGMLAREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTIWGEDASEFKPE 441

Query: 470 RFSEGISKATKNQI 484
           RF+EGISKATKNQ+
Sbjct: 442 RFAEGISKATKNQV 452

[Close]

BLAST of Moc08g21760 vs. ExPASy TrEMBL
Match: A0A6J1E9Z3 (cytochrome P450 CYP72A219-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432119 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.5e-194
Identity = 336/434 (77.42%), Postives = 380/434 (87.56%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ + PK+LEK L  QGLAGNSYRLLFGDS D AAA+R+A++  P+SFSH IA RATPS
Sbjct: 44  NWMWLTPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKT-QPISFSHRIAARATPS 103

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           +H+TIH+YGKD FTW G TPRVYI  PEQ+K+VFSQINDIRK++ FP    RRMG G+V+
Sbjct: 104 SHITIHRYGKDSFTWIGPTPRVYITHPEQVKVVFSQINDIRKATLFPFR--RRMGGGVVS 163

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMI+KWE M+ K+G CELDVWPDLQN
Sbjct: 164 LEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKEGCCELDVWPDLQN 223

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           M ADVISRTAFGSSYEEGKKIFQLLK+WA LLM  +   VY+IPG  FLPTKLNKR +EI
Sbjct: 224 MAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLRFLPTKLNKRMDEI 283

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           +  IRDM+ GII+KRQ+AMK  EASNDDLLGILLESN+ +IQE+ N+KDVGMS+++VI+E
Sbjct: 284 NGNIRDMIWGIINKRQNAMKKGEASNDDLLGILLESNSREIQEHKNKKDVGMSMEEVIAE 343

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLFYFAGQETTAALL WTMVLLG +SEWQDRARAEVLE+F DN K DFDGLSRLKIVTM
Sbjct: 344 CRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNKKFDFDGLSRLKIVTM 403

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           ILNEVLRLYPPVG+L+REIH ETKLGNLTLPVG SIGIPIV IHQDP +WGEDA EFKPE
Sbjct: 404 ILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTLWGEDASEFKPE 463

Query: 470 RFSEGISKATKNQI 484
           RF EGISKATKNQ+
Sbjct: 464 RFVEGISKATKNQV 474

[Close]

BLAST of Moc08g21760 vs. ExPASy TrEMBL
Match: A0A6J1EFS6 (cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432119 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.5e-194
Identity = 336/434 (77.42%), Postives = 380/434 (87.56%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ + PK+LEK L  QGLAGNSYRLLFGDS D AAA+R+A++  P+SFSH IA RATPS
Sbjct: 44  NWMWLTPKRLEKCLRHQGLAGNSYRLLFGDSKDTAAAVRQAKT-QPISFSHRIAARATPS 103

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           +H+TIH+YGKD FTW G TPRVYI  PEQ+K+VFSQINDIRK++ FP    RRMG G+V+
Sbjct: 104 SHITIHRYGKDSFTWIGPTPRVYITHPEQVKVVFSQINDIRKATLFPFR--RRMGGGVVS 163

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMI+KWE M+ K+G CELDVWPDLQN
Sbjct: 164 LEGAKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMINKWEMMITKEGCCELDVWPDLQN 223

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           M ADVISRTAFGSSYEEGKKIFQLLK+WA LLM  +   VY+IPG  FLPTKLNKR +EI
Sbjct: 224 MAADVISRTAFGSSYEEGKKIFQLLKQWASLLMAYVMKGVYFIPGLRFLPTKLNKRMDEI 283

Query: 290 DKKIRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISE 349
           +  IRDM+ GII+KRQ+AMK  EASNDDLLGILLESN+ +IQE+ N+KDVGMS+++VI+E
Sbjct: 284 NGNIRDMIWGIINKRQNAMKKGEASNDDLLGILLESNSREIQEHKNKKDVGMSMEEVIAE 343

Query: 350 CRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTM 409
           CRLFYFAGQETTAALL WTMVLLG +SEWQDRARAEVLE+F DN K DFDGLSRLKIVTM
Sbjct: 344 CRLFYFAGQETTAALLAWTMVLLGRYSEWQDRARAEVLEIFGDNKKFDFDGLSRLKIVTM 403

Query: 410 ILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPE 469
           ILNEVLRLYPPVG+L+REIH ETKLGNLTLPVG SIGIPIV IHQDP +WGEDA EFKPE
Sbjct: 404 ILNEVLRLYPPVGLLSREIHKETKLGNLTLPVGVSIGIPIVCIHQDPTLWGEDASEFKPE 463

Query: 470 RFSEGISKATKNQI 484
           RF EGISKATKNQ+
Sbjct: 464 RFVEGISKATKNQV 474

[Close]

BLAST of Moc08g21760 vs. ExPASy TrEMBL
Match: A0A1S3B801 (cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103486999 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 4.0e-192
Identity = 335/435 (77.01%), Postives = 383/435 (88.05%), Query Frame = 0


Query: 50  SYLIVEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPS 109
           +++ + PK+LEK L QQGLAGNSYR LFGD+ +I  A+R+ARS   M+FSHDIA RATPS
Sbjct: 30  NWVWLRPKRLEKLLRQQGLAGNSYRFLFGDTKEIGVAVRQARS-QSMTFSHDIASRATPS 89

Query: 110 AHLTIHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVT 169
           ++ TIHKYGKD FTW GTTPRVYI +PEQ+KIVFSQINDIRK+SSFP  + RRMGSGLVT
Sbjct: 90  SYPTIHKYGKDSFTWIGTTPRVYITEPEQVKIVFSQINDIRKTSSFP--LRRRMGSGLVT 149

Query: 170 LEGPKWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVLKDGSCELDVWPDLQN 229
           LEG KWAKHRKIINPAFHMEKLK+MFPAFS+SC EM++KWE M+ + GS E+DVWPDLQN
Sbjct: 150 LEGSKWAKHRKIINPAFHMEKLKEMFPAFSKSCREMVNKWEKMISEKGSGEIDVWPDLQN 209

Query: 230 MTADVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEI 289
           + ADVISRTAFGSSY+EGKKIFQLLKEWAVLLM+ LT R YYIPG  ++PTKLNKR +EI
Sbjct: 210 LAADVISRTAFGSSYDEGKKIFQLLKEWAVLLMSYLTKRAYYIPGARYIPTKLNKRMQEI 269

Query: 290 DKKIRDMVLGIISKRQSAMKADEAS-NDDLLGILLESNASQIQENGNRKDVGMSIQDVIS 349
           D KIRD+V GII+KRQ+AMK  EAS N+DLLGILLESN +QI+E  N+KDVGMSI++VIS
Sbjct: 270 DMKIRDLVRGIINKRQNAMKKGEASKNEDLLGILLESNETQIEEQKNKKDVGMSIEEVIS 329

Query: 350 ECRLFYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVT 409
           ECRLFYFAGQETTA LL WTMVLLG +SEWQDRARAEVLEVF +N KLDFDGLSRL++V+
Sbjct: 330 ECRLFYFAGQETTAVLLAWTMVLLGRYSEWQDRARAEVLEVFGNNKKLDFDGLSRLRVVS 389

Query: 410 MILNEVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKP 469
           MILNEVLRLYPPVGMLARE+H ETKLGNLTLP G SIGIPI+ +HQ+PKIWGED  EF P
Sbjct: 390 MILNEVLRLYPPVGMLAREVHNETKLGNLTLPSGVSIGIPILSMHQNPKIWGEDVLEFNP 449

Query: 470 ERFSEGISKATKNQI 484
           ERFSEGISKATKNQ+
Sbjct: 450 ERFSEGISKATKNQV 461

[Close]

BLAST of Moc08g21760 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 224/432 (51.85%), Postives = 301/432 (69.68%), Query Frame = 0


Query: 54  VEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPSAHLT 113
           ++PK LE  L +QGL G  Y  L GD       M  ARS  P++ + DI PR  P A   
Sbjct: 29  IKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARS-KPINVTDDITPRLLPLALKM 88

Query: 114 IHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVTLEGP 173
           ++ +GK  F W G  P + I +PEQ+K VF+++ND  K+S+FP  ++R +  GL + +G 
Sbjct: 89  LNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDFEKASTFP--LIRLLAGGLASYKGD 148

Query: 174 KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVL-KDGSCELDVWPDLQNMTA 233
           KWA HR+IINPAFH+EK+K+M PAF   CSE++ +WE +   K+   E+DVWP L NMTA
Sbjct: 149 KWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLFTDKESPLEVDVWPWLVNMTA 208

Query: 234 DVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEIDKK 293
           DVIS TAFGSSY+EG++IFQL  E A L+      +  YIPG  F PTK N+R + ID++
Sbjct: 209 DVISHTAFGSSYKEGQRIFQLQGELAELIAQAF--KKSYIPGSRFYPTKSNRRMKAIDRE 268

Query: 294 IRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISECRL 353
           +  ++ GI+SKR+ A +A E +NDDLLGILLESN+ + Q N      GMS++DV+ EC+L
Sbjct: 269 VDVILRGIVSKREKAREAGEPANDDLLGILLESNSEESQGN------GMSVEDVMKECKL 328

Query: 354 FYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTMILN 413
           FYFAGQETT+ LLVWTMVLL HH +WQ RAR EV++V  +NNK D + L+ LK++TMI N
Sbjct: 329 FYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPDMESLNNLKVMTMIFN 388

Query: 414 EVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPERFS 473
           EVLRLYPPV  L R ++ E KLG LTLP G  I +P + + +D ++WG+DA +FKPERF 
Sbjct: 389 EVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTELWGDDAADFKPERFR 448

Query: 474 EGISKATKNQIA 485
           +G+SKATKNQ++
Sbjct: 449 DGLSKATKNQVS 449

[Close]

BLAST of Moc08g21760 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 446.0 bits (1146), Expect = 4.2e-125
Identity = 222/430 (51.63%), Postives = 305/430 (70.93%), Query Frame = 0


Query: 56  PKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPSAHLTIH 115
           PK LE+ L +QGL+G SY  L GD   + +    A S  P+  + DI PR  P     + 
Sbjct: 32  PKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATS-KPIKPTDDITPRVMPHPLQMLK 91

Query: 116 KYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVTLEGPKW 175
            +G+   TWFG  P + I DPEQ+K VF+++ D +K+ +FP + +  +G+GLV+ +G KW
Sbjct: 92  THGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFPLSKI--LGTGLVSYDGDKW 151

Query: 176 AKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVL-KDGSCELDVWPDLQNMTADV 235
           A+HR+IINPAFH+EK+K+M   F +SCSE++ +W+ +V  K  SCE+DVWP L +MTADV
Sbjct: 152 AQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGSSCEVDVWPGLTSMTADV 211

Query: 236 ISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEIDKKIR 295
           ISRTAFGSSY EG +IF+L  E A L+M     + ++IPG+ +LPTK N+R +   ++I+
Sbjct: 212 ISRTAFGSSYREGHRIFELQAELAQLVMQAF--QKFFIPGYIYLPTKGNRRMKTAAREIQ 271

Query: 296 DMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISECRLFY 355
           D++ GII+KR+ A ++ EA ++DLLGILLESN  Q + N      GMS +D++ EC+LFY
Sbjct: 272 DILRGIINKRERARESGEAPSEDLLGILLESNLGQTEGN------GMSTEDMMEECKLFY 331

Query: 356 FAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTMILNEV 415
            AGQETT+ LLVWTMVLL  H +WQ RAR EV +VF D    D +GL++LK++TMIL EV
Sbjct: 332 LAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQP-DTEGLNQLKVMTMILYEV 391

Query: 416 LRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPERFSEG 475
           LRLYPPV  L R IH E KLG+LTLP G  I +P++ +H+D ++WG DAGEFKPERF +G
Sbjct: 392 LRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPERFKDG 449

Query: 476 ISKATKNQIA 485
           +SKATKNQ++
Sbjct: 452 LSKATKNQVS 449

[Close]

BLAST of Moc08g21760 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 443.0 bits (1138), Expect = 3.5e-124
Identity = 223/432 (51.62%), Postives = 304/432 (70.37%), Query Frame = 0


Query: 54  VEPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPSAHLT 113
           ++PK LE  L +QGLAG  Y  L GD     + +  ARS  P++ + DI PR  P     
Sbjct: 30  LKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEARS-KPINLTDDITPRIVPYPLQM 89

Query: 114 IHKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVTLEGP 173
           +  +G+  FTWFG  P + I DPEQ+K VF+++ D +K+ +FP  + R + +GLV+ +G 
Sbjct: 90  LKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTFP--LGRLIAAGLVSYDGD 149

Query: 174 KWAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVL-KDGSCELDVWPDLQNMTA 233
           KW KHR+IINPAFH+EK+K+M PAF QSCSE++ +W+ +V  K  SCE+D+WP L +MTA
Sbjct: 150 KWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDKQSSCEVDIWPWLVSMTA 209

Query: 234 DVISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEIDKK 293
           DVISRTAFGSSY+EG++IF+L  E A L++      +  IPG+ + PTK N+R +   ++
Sbjct: 210 DVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAI--IPGYRYFPTKGNRRMKAAARE 269

Query: 294 IRDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISECRL 353
           I+ ++ GI++KR  A +A EA +DDLLGILLESN  Q + N      GMS ++++ EC+L
Sbjct: 270 IKFILRGIVNKRLRAREAGEAPSDDLLGILLESNLGQTKGN------GMSTEELMEECKL 329

Query: 354 FYFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTMILN 413
           FYFAGQETT  LLVWTMVLL  H +WQ RAR EV +VF D    D +GL++LK++TMIL 
Sbjct: 330 FYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEP-DAEGLNQLKVMTMILY 389

Query: 414 EVLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPERFS 473
           EVLRLYPPV  L R IH E +LG+LTLP G  I +PI+ I +D ++WG DAGEFKP+RF 
Sbjct: 390 EVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDRELWGNDAGEFKPDRFK 449

Query: 474 EGISKATKNQIA 485
           +G+SKATKNQ++
Sbjct: 450 DGLSKATKNQVS 449

[Close]

BLAST of Moc08g21760 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 441.4 bits (1134), Expect = 1.0e-123
Identity = 224/431 (51.97%), Postives = 297/431 (68.91%), Query Frame = 0


Query: 55  EPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPSAHLTI 114
           +PK LE  L +QGLAG  Y  L GD       +  ARS  P+  + DI+PR  P      
Sbjct: 31  KPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARS-KPLKLTDDISPRVVPYPLQMF 90

Query: 115 HKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVTLEGPK 174
             YG+  FTWFG  P + I DPEQ+K VF+++ D +K  +FP   +  +  GL   +G K
Sbjct: 91  KTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHTFPLATI--IAKGLANYDGDK 150

Query: 175 WAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVL-KDGSCELDVWPDLQNMTAD 234
           WAKHR+IINPAFH+EK+K+M PAF QSC E++ +W+ +V  K  SCE+DVWP L +MTAD
Sbjct: 151 WAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGSSCEVDVWPGLVSMTAD 210

Query: 235 VISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEIDKKI 294
           VISRTAFGSSY+EG++IF+L  E A L++     R  +IPG+S+LPTK N+R +   ++I
Sbjct: 211 VISRTAFGSSYKEGQRIFELQAELAQLIIQAF--RKAFIPGYSYLPTKSNRRMKAAAREI 270

Query: 295 RDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISECRLF 354
           + ++ GI++KR  A +A EA +DDLLGILLESN  Q + N      GMS +D++ EC+LF
Sbjct: 271 QVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQTEGN------GMSTEDLMEECKLF 330

Query: 355 YFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTMILNE 414
           YFAGQETT+ LLVWTMVLL  H +WQ RAR EV +VF D    D +GL++LK++TMIL E
Sbjct: 331 YFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEP-DAEGLNQLKVMTMILYE 390

Query: 415 VLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPERFSE 474
           VLRLYPPV  L R IH E KLG+LTLP G  I +PI+ +  D ++WG DA EF P+RF +
Sbjct: 391 VLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWGNDAAEFNPDRFKD 449

Query: 475 GISKATKNQIA 485
           G+SKATK+Q++
Sbjct: 451 GLSKATKSQVS 449

[Close]

BLAST of Moc08g21760 vs. TAIR 10
Match: AT3G14650.1 (cytochrome P450, family 72, subfamily A, polypeptide 11 )

HSP 1 Score: 439.9 bits (1130), Expect = 3.0e-123
Identity = 224/429 (52.21%), Postives = 303/429 (70.63%), Query Frame = 0


Query: 55  EPKQLEKRLTQQGLAGNSYRLLFGDSNDIAAAMRRARSGPPMSFSHDIAPRATPSAHLTI 114
           +PK LE  L +QGLAG  Y  L GD     +    ARS  P++ + DI PR  P     +
Sbjct: 31  KPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARS-KPINLTDDITPRIVPYPLQML 90

Query: 115 HKYGKDCFTWFGTTPRVYIRDPEQLKIVFSQINDIRKSSSFPANIVRRMGSGLVTLEGPK 174
             +G+  FTWFG  P + I DPEQ+  V +++ D +K+ +FP  + R + +G+++ +G K
Sbjct: 91  KTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKAHTFP--LGRLIATGVLSYDGDK 150

Query: 175 WAKHRKIINPAFHMEKLKDMFPAFSQSCSEMISKWETMVL-KDGSCELDVWPDLQNMTAD 234
           WAKHR+IINPAFH+EK+K+M PAF QSCSE++ KW+ +V  K+ SCE+DVWP L +MTAD
Sbjct: 151 WAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDKESSCEVDVWPGLVSMTAD 210

Query: 235 VISRTAFGSSYEEGKKIFQLLKEWAVLLMTRLTNRVYYIPGWSFLPTKLNKRTEEIDKKI 294
           VISRTAFGSS  EG++IF+L  E A L++   T R  +IPG+S+LPTK N+R +   ++I
Sbjct: 211 VISRTAFGSSCVEGQRIFELQAELAQLIIQ--TVRKAFIPGYSYLPTKGNRRMKAKAREI 270

Query: 295 RDMVLGIISKRQSAMKADEASNDDLLGILLESNASQIQENGNRKDVGMSIQDVISECRLF 354
           + ++ GI++KR  A +A EA NDDLLGILLESN  Q + N      GMS +D++ EC+LF
Sbjct: 271 QVILRGIVNKRLRAREAGEAPNDDLLGILLESNLGQTKGN------GMSTEDLMEECKLF 330

Query: 355 YFAGQETTAALLVWTMVLLGHHSEWQDRARAEVLEVFSDNNKLDFDGLSRLKIVTMILNE 414
           YF GQETT+ LLVWTMVLL  H +WQ RAR EV +VF D    D +GL++LK++TMIL E
Sbjct: 331 YFVGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEP-DAEGLNQLKVMTMILYE 390

Query: 415 VLRLYPPVGMLAREIHTETKLGNLTLPVGASIGIPIVYIHQDPKIWGEDAGEFKPERFSE 474
           VLRLYPP+  L+R IH E +LG+LTLP G  I +PI+ + +D ++WG DAGEFKP+RF +
Sbjct: 391 VLRLYPPIPQLSRAIHKEMELGDLTLPGGVLINLPILLVQRDTELWGNDAGEFKPDRFKD 447

Query: 475 GISKATKNQ 483
           G+SKATKNQ
Sbjct: 451 GLSKATKNQ 447

The following BLAST results are available for this feature:

BLAST of Moc08g21760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158406.1	1.2e-248	95.78	11-oxo-beta-amyrin 30-oxidase-like [Momordica charantia]	[more]
XP_023006210.1	4.2e-196	78.80	cytochrome P450 CYP72A219-like [Cucurbita maxima]	[more]
XP_023529493.1	3.6e-195	78.57	cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo]	[more]
XP_022924709.1	5.2e-194	77.42	cytochrome P450 CYP72A219-like isoform X2 [Cucurbita moschata]	[more]
XP_022924700.1	5.2e-194	77.42	cytochrome P450 CYP72A219-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Moc08g21760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0S2IHL2	4.2e-130	54.61	Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1	[more]
H1A988	5.5e-130	55.53	11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=...	[more]
Q2MJ19	3.9e-128	51.95	Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1	[more]
H1A981	3.3e-127	52.34	11-oxo-beta-amyrin 30-oxidase OS=Medicago truncatula OX=3880 GN=CYP72A63 PE=1 SV...	[more]
W8JWW3	8.2e-126	53.92	Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1	[more]

BLAST of Moc08g21760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0T8	6.0e-249	95.78	11-oxo-beta-amyrin 30-oxidase-like OS=Momordica charantia OX=3673 GN=LOC11102489...	[more]
A0A6J1KZI7	2.0e-196	78.80	cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111499007 PE=3 ...	[more]
A0A6J1E9Z3	2.5e-194	77.42	cytochrome P450 CYP72A219-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1EFS6	2.5e-194	77.42	cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A1S3B801	4.0e-192	77.01	cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103486999 PE=3 SV=1	[more]

BLAST of Moc08g21760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14610.1	1.2e-127	51.85	cytochrome P450, family 72, subfamily A, polypeptide 7	[more]
AT3G14680.1	4.2e-125	51.63	cytochrome P450, family 72, subfamily A, polypeptide 14	[more]
AT3G14660.1	3.5e-124	51.62	cytochrome P450, family 72, subfamily A, polypeptide 13	[more]
AT3G14690.1	1.0e-123	51.97	cytochrome P450, family 72, subfamily A, polypeptide 15	[more]
AT3G14650.1	3.0e-123	52.21	cytochrome P450, family 72, subfamily A, polypeptide 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 356..373 score: 38.35 coord: 409..420 score: 40.16
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 115..474 e-value: 1.2E-65 score: 222.1
IPR002402	Cytochrome P450, E-class, group II	PRINTS	PR00464	EP450II	coord: 442..457 score: 31.25 coord: 172..192 score: 37.03 coord: 229..247 score: 31.47
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 105..496 e-value: 1.7E-87 score: 295.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 113..474
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 480..504
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..37
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 48..484
None	No IPR available	PANTHER	PTHR24282:SF225	CYTOCHROME	coord: 48..484

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g21760.1	Moc08g21760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen