Moc08g18560 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g18560
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCCHC-type domain-containing protein
Locationchr8: 14026256 .. 14027864 (-)
RNA-Seq ExpressionMoc08g18560
SyntenyMoc08g18560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTAATCTGGAAATCCTACAACCGCCGAGGAAACAGTGTCCCAACTTCGACGATTCATATCTTACTGACCTTTTTGCTGAATATAGTCAAATCAGGTACGCACTTAAAGGTCTTTGGTCCTCGCCAACCAAGGTCTGTAGCCGAAGTTATTGACGAAAGAAGAGAAGGTGTCCAAGCAGAAAACTTTCCATATTTGAAGCTGGACGTTTTTGGAAAAATTATTTGGGCTATCAAATCTTGTGCAAATTGATTTCTTTTTGGCAAGTAATCCTAACACTGGTTATATTTTGATTTAAAATTATTGTTGAATTATTTTGACCTAGTTGGATAGCCCAAAAGAAGGAGGACAACCGCCACCCCCACTAGGCCACCTGAGAGAGTCCAATTGTGCAGCCCCAGAATTTTCTTTGAGAGAGCAGCCCCCTCCAACGAAATCCAAACCTTTTAGCAACCTACAAACTCTAATTACACTTGTTGTGCTAATGTTTGCCACGGTTTGGAGCCCCATCCGAGTTGTATACATCACACACTATTCATGTAAAGTCACCCTATTTACCGCAAGGGTTACTTCTTTGTTGGTAAATATTATTCTTGTGTGTGTTTGCACTACAAATTGGATTTTATACATCTTTTTGGTTTATTCTTCATTTCATTGATTTTATTTGCATAAGTCTTTCTTTGTTTAGCTTTAAATTCAATATGTATATTGTTGTTAAGTCTTTAGAACCAAGAGCATTAGGCTTAAGGTTGTCATTATCACTCTTGTTCATATCTTGCCTTATTCTTCAAATATCATTTGGTATCATTATAACTATTATTTGGTATTCTTTTGAGTCTTGTCTTTGGTGTCTTCTATACCATTGACCCCTAGTAAAAGAAAATATATATATATATATATATATATATATATTTTTGCAAGTTGCTTGTAGGCTTATACTCACTTGTTCTAAGACCGATTCGCCACGAGCATTCAAGCAAGCATCTCCAAAGGGTATTCACAACAACATCCACCCTACCAAAGGAGAGGAAGCCGAGAGGACAAGTAAGGGAGTGAAAGTGAATTTTTTCTTTGTTTATACTAACCTTTGCTTGTGTAGTGATGGCCATGAAAGGCAAATCTCAAGGAGAAGGAACAAGTCCACGATGCAAGAATTATAATAACCATGTCTTTAATGTAACTCATCAAACGAGGAAAGATCACCATAATAGTGGCTACCTTCGACACACAACTCCTAGACTTCCTAAGTTCCATGGAAGTAGAAAGCCAGAAGAGTATCTTCATTGGTAAGACTAAGTCGAGAGCATCTCTGCACAACACCATTGGAGTGAAAGCATCAAAGTAAATCTTATTATCACTTGTTTTAAAGATTATGCTATAACATGGTGGGATGCTTTGTTTTTTAGTAGGAAAAGGAATCGCGAAAGTCAAATCACCTCGTGGAGAGAACTCAAGCCATTAATGAGGAGACAATTTGTTTCTAAGTCATTAGTCAAGTTGAAAAACTTGAGACAAAACGACAAAAATGTTGAAGCCTACTATGAAGAGATGTGCTCATTGCTTGAGGAGCTTAACGTTTATGAAGATGAGGAGAGCACCATGGATTGA

mRNA sequence

ATGTGTAATCTGGAAATCCTACAACCGCCGAGGAAACAGTGTCCCAACTTCGACGATTCATATCTTACTGACCTTTTTGCTGAATATAGTCAAATCAGGCTTATACTCACTTGTTCTAAGACCGATTCGCCACGAGCATTCAAGCAAGCATCTCCAAAGGGTATTCACAACAACATCCACCCTACCAAAGGAGAGGAAGCCGAGAGGACAATGATGGCCATGAAAGGCAAATCTCAAGGAGAAGGAACAAGTCCACGATGCAAGAATTATAATAACCATGTCTTTAATGTAACTCATCAAACGAGGAAAGATCACCATAATAGTGGCTACCTTCGACACACAACTCCTAGACTTCCTAAGTTCCATGGAAGTAGAAAGCCAGAAGAGTATCTTCATTGGAAAAGGAATCGCGAAAGTCAAATCACCTCGTGGAGAGAACTCAAGCCATTAATGAGGAGACAATTTGTTTCTAAGTCATTAGTCAAGTTGAAAAACTTGAGACAAAACGACAAAAATGTTGAAGCCTACTATGAAGAGATGTGCTCATTGCTTGAGGAGCTTAACGTTTATGAAGATGAGGAGAGCACCATGGATTGA

Coding sequence (CDS)

ATGTGTAATCTGGAAATCCTACAACCGCCGAGGAAACAGTGTCCCAACTTCGACGATTCATATCTTACTGACCTTTTTGCTGAATATAGTCAAATCAGGCTTATACTCACTTGTTCTAAGACCGATTCGCCACGAGCATTCAAGCAAGCATCTCCAAAGGGTATTCACAACAACATCCACCCTACCAAAGGAGAGGAAGCCGAGAGGACAATGATGGCCATGAAAGGCAAATCTCAAGGAGAAGGAACAAGTCCACGATGCAAGAATTATAATAACCATGTCTTTAATGTAACTCATCAAACGAGGAAAGATCACCATAATAGTGGCTACCTTCGACACACAACTCCTAGACTTCCTAAGTTCCATGGAAGTAGAAAGCCAGAAGAGTATCTTCATTGGAAAAGGAATCGCGAAAGTCAAATCACCTCGTGGAGAGAACTCAAGCCATTAATGAGGAGACAATTTGTTTCTAAGTCATTAGTCAAGTTGAAAAACTTGAGACAAAACGACAAAAATGTTGAAGCCTACTATGAAGAGATGTGCTCATTGCTTGAGGAGCTTAACGTTTATGAAGATGAGGAGAGCACCATGGATTGA

Protein sequence

MCNLEILQPPRKQCPNFDDSYLTDLFAEYSQIRLILTCSKTDSPRAFKQASPKGIHNNIHPTKGEEAERTMMAMKGKSQGEGTSPRCKNYNNHVFNVTHQTRKDHHNSGYLRHTTPRLPKFHGSRKPEEYLHWKRNRESQITSWRELKPLMRRQFVSKSLVKLKNLRQNDKNVEAYYEEMCSLLEELNVYEDEESTMD
Homology
BLAST of Moc08g18560 vs. NCBI nr
Match: XP_022159097.1 (uncharacterized protein LOC111025539 [Momordica charantia])

HSP 1 Score: 60.5 bits (145), Expect = 1.9e-05
Identity = 39/106 (36.79%), Postives = 64/106 (60.38%), Query Frame = 0

Query: 102 RKDHHNSGYLRHTTPRLPKFHGSRKPEEYLHWKRNRE-----SQITSWRELKPLMRRQFV 161
           R+D+H      +   + PKFHG+   E+YL W++  E     + +T   E K LMRR+F+
Sbjct: 87  REDNHVDRGFGNIKVKFPKFHGTSNSEDYLEWEQKAEHIFYGNNLT--EEKKELMRRRFI 146

Query: 162 SKSLV-----KLKNLRQNDKNVEAYYEEMCSLLEELNVYEDEESTM 198
               +     KL+ LRQ +K+VE YY++M +L+E +++ E+EE+TM
Sbjct: 147 PPHFLRDCAQKLQALRQGNKSVEEYYKKMETLMERVDIDENEENTM 190

BLAST of Moc08g18560 vs. NCBI nr
Match: XP_023542108.1 (glutamic acid-rich protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 60.1 bits (144), Expect = 2.5e-05
Identity = 37/99 (37.37%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 117 RLPKFHGSRKPEEYL-------------HWKRNRESQITSWRELKPLMRRQFVSKSL--- 176
           ++P F+    P EYL              W+RN E  I +W ELK LMR++++ K     
Sbjct: 215 KIPTFYERSDPYEYLQYESKIEHTILKSEWRRNYEEPIEAWEELKTLMRKRYIPKHYSRV 274

Query: 177 --VKLKNLRQNDKNVEAYYEEMCSLLEELNVYEDEESTM 198
              KL  L+Q  K+VE YY+EM +L+   ++ EDEE TM
Sbjct: 275 LKQKLYTLQQGSKSVEEYYKEMETLMNRASIDEDEEDTM 313

BLAST of Moc08g18560 vs. NCBI nr
Match: XP_038888883.1 (uncharacterized protein LOC120078660 [Benincasa hispida])

HSP 1 Score: 58.2 bits (139), Expect = 9.6e-05
Identity = 31/72 (43.06%), Postives = 44/72 (61.11%), Query Frame = 0

Query: 131 LHWKRNRESQITSWRELKPLMRRQFVSKSLV-----KLKNLRQNDKNVEAYYEEMCSLLE 190
           L W+RN E  I +W ELK LMR+ ++ K  +     KL +L+Q  K V+ YY+EM +L+ 
Sbjct: 175 LEWRRNYEELIETWEELKALMRKMYIPKHYIRELKQKLYSLQQGSKGVDDYYKEMQTLMN 234

Query: 191 ELNVYEDEESTM 198
             N+ EDEE  M
Sbjct: 235 RENIDEDEEDAM 246

BLAST of Moc08g18560 vs. NCBI nr
Match: XP_027090397.1 (uncharacterized protein LOC113711430 [Coffea arabica])

HSP 1 Score: 58.2 bits (139), Expect = 9.6e-05
Identity = 35/97 (36.08%), Postives = 51/97 (52.58%), Query Frame = 0

Query: 117 RLPKFHGSRKPEEYLHW-----------KRNRESQITSWRELKPLMRRQFVSKSLV---- 176
           ++P F G   PE YL W           +RNRE QI++W EL+  MR++FV         
Sbjct: 91  KIPAFQGKSSPEAYLEWEQRIEMERTSRRRNRERQISTWEELRTTMRKRFVPSHYYRDLY 150

Query: 177 -KLKNLRQNDKNVEAYYEEMCSLLEELNVYEDEESTM 198
            +L+ L Q  + VE YY+EM   +   ++ ED E+TM
Sbjct: 151 QRLQTLVQGSRTVEDYYKEMEITMLRADIVEDREATM 187

BLAST of Moc08g18560 vs. NCBI nr
Match: KAE8718649.1 (hypothetical protein F3Y22_tig00110007pilonHSYRG00200 [Hibiscus syriacus])

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-04
Identity = 41/119 (34.45%), Postives = 62/119 (52.10%), Query Frame = 0

Query: 94  VFNVTHQTRKDHHNSGYLRHTTPR--LPKFHGSRKPEEYLHW--------KRNRESQITS 153
           V  +T   R D H +     +T +  +P F G   PE YL W        +RN E  I++
Sbjct: 460 VEQITPGARNDVHRNKNDNFSTIKVTIPPFQGRTDPEAYLDWWDQLESSRRRNSECPIST 519

Query: 154 WRELKPLMRRQFVSKS-----LVKLKNLRQNDKNVEAYYEEMCSLLEELNVYEDEESTM 198
           W E+K +MR++FV          KL+NL+Q + ++E Y++EM   +   NV ED E+TM
Sbjct: 520 WEEMKAIMRKRFVPTHYHLDLYHKLQNLKQENHSIEDYFKEMEIAMIRANVDEDREATM 578

BLAST of Moc08g18560 vs. ExPASy TrEMBL
Match: A0A6J1DYV9 (uncharacterized protein LOC111025539 OS=Momordica charantia OX=3673 GN=LOC111025539 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.4e-06
Identity = 39/106 (36.79%), Postives = 64/106 (60.38%), Query Frame = 0

Query: 102 RKDHHNSGYLRHTTPRLPKFHGSRKPEEYLHWKRNRE-----SQITSWRELKPLMRRQFV 161
           R+D+H      +   + PKFHG+   E+YL W++  E     + +T   E K LMRR+F+
Sbjct: 87  REDNHVDRGFGNIKVKFPKFHGTSNSEDYLEWEQKAEHIFYGNNLT--EEKKELMRRRFI 146

Query: 162 SKSLV-----KLKNLRQNDKNVEAYYEEMCSLLEELNVYEDEESTM 198
               +     KL+ LRQ +K+VE YY++M +L+E +++ E+EE+TM
Sbjct: 147 PPHFLRDCAQKLQALRQGNKSVEEYYKKMETLMERVDIDENEENTM 190

BLAST of Moc08g18560 vs. ExPASy TrEMBL
Match: A0A2N9GWZ3 (Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34749 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.6e-05
Identity = 35/84 (41.67%), Postives = 52/84 (61.90%), Query Frame = 0

Query: 120 KFHGSRKPEE-YLHWKRNRESQITSWRELKPLMRRQFV-----SKSLVKLKNLRQNDKNV 179
           +F G+R  E+  L W+RNRE  I +W E+K +MR++F+      +   KL+ LRQ  ++V
Sbjct: 100 RFGGARNREDNNLRWRRNRERPIDTWEEMKVVMRKRFIPSYYYRELYKKLQGLRQGSRSV 159

Query: 180 EAYYEEMCSLLEELNVYEDEESTM 198
           E YY+EM   +   NV ED E+TM
Sbjct: 160 EDYYKEMEIAMIRANVEEDREATM 183

BLAST of Moc08g18560 vs. ExPASy TrEMBL
Match: A0A2N9HCQ9 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS37590 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.6e-05
Identity = 35/84 (41.67%), Postives = 52/84 (61.90%), Query Frame = 0

Query: 120 KFHGSRKPEE-YLHWKRNRESQITSWRELKPLMRRQFV-----SKSLVKLKNLRQNDKNV 179
           +F G+R  E+  L W+RNRE  I +W E+K +MR++F+      +   KL+ LRQ  ++V
Sbjct: 89  RFGGARNREDNNLRWRRNRERPIDTWEEMKVVMRKRFIPSYYYRELYKKLQGLRQGSRSV 148

Query: 180 EAYYEEMCSLLEELNVYEDEESTM 198
           E YY+EM   +   NV ED E+TM
Sbjct: 149 EDYYKEMEIAMIRANVEEDREATM 172

BLAST of Moc08g18560 vs. ExPASy TrEMBL
Match: A0A6A3BUU6 (CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110007pilonHSYRG00200 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 7.9e-05
Identity = 41/119 (34.45%), Postives = 62/119 (52.10%), Query Frame = 0

Query: 94  VFNVTHQTRKDHHNSGYLRHTTPR--LPKFHGSRKPEEYLHW--------KRNRESQITS 153
           V  +T   R D H +     +T +  +P F G   PE YL W        +RN E  I++
Sbjct: 460 VEQITPGARNDVHRNKNDNFSTIKVTIPPFQGRTDPEAYLDWWDQLESSRRRNSECPIST 519

Query: 154 WRELKPLMRRQFVSKS-----LVKLKNLRQNDKNVEAYYEEMCSLLEELNVYEDEESTM 198
           W E+K +MR++FV          KL+NL+Q + ++E Y++EM   +   NV ED E+TM
Sbjct: 520 WEEMKAIMRKRFVPTHYHLDLYHKLQNLKQENHSIEDYFKEMEIAMIRANVDEDREATM 578

BLAST of Moc08g18560 vs. ExPASy TrEMBL
Match: A0A5P1ESV5 (Retrotrans_gag domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C05F8650 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 6.7e-04
Identity = 30/70 (42.86%), Postives = 45/70 (64.29%), Query Frame = 0

Query: 133 WKRNRESQITSWRELKPLMRRQFVSKSLV-----KLKNLRQNDKNVEAYYEEMCSLLEEL 192
           W+RNRES I  W E+K +MR++FV          KL++LRQ +++VE YY+E+   +   
Sbjct: 39  WRRNRESPIKMWEEMKRVMRKRFVPSYYYRELYNKLQSLRQGNRSVEEYYKEIEVAMIRA 98

Query: 193 NVYEDEESTM 198
           NV ED E+T+
Sbjct: 99  NVEEDREATI 108

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159097.11.9e-0536.79uncharacterized protein LOC111025539 [Momordica charantia][more]
XP_023542108.12.5e-0537.37glutamic acid-rich protein-like [Cucurbita pepo subsp. pepo][more]
XP_038888883.19.6e-0543.06uncharacterized protein LOC120078660 [Benincasa hispida][more]
XP_027090397.19.6e-0536.08uncharacterized protein LOC113711430 [Coffea arabica][more]
KAE8718649.11.6e-0434.45hypothetical protein F3Y22_tig00110007pilonHSYRG00200 [Hibiscus syriacus][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DYV99.4e-0636.79uncharacterized protein LOC111025539 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A2N9GWZ33.6e-0541.67Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34749 PE=4 SV=1[more]
A0A2N9HCQ93.6e-0541.67Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS37590 PE=4 SV=1[more]
A0A6A3BUU67.9e-0534.45CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig0... [more]
A0A5P1ESV56.7e-0442.86Retrotrans_gag domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005162Retrotransposon gag domainPFAMPF03732Retrotrans_gagcoord: 133..195
e-value: 2.6E-7
score: 30.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g18560.1Moc08g18560.1mRNA