Moc08g17370 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g17370
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr8: 13199669 .. 13200912 (-)
RNA-Seq Expression	Moc08g17370
Synteny	Moc08g17370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGTGGACTTCATTAGGGGCAACTCACCACAAGACCCCAAGGAGCGCAGAAAATTGGCAAGGCGGGCAGCTCGGTTCGTGGTCCGAGATGGGGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAGATGCCTAACCCCTGAAGAGGGCCTGTACGTCCTCAGAGAGATCCACGAAGGAGTGTGTGGCAATCACTCGGGCGCCCGGTCGCTGTCAGCCAAAGTGATCCGACAAGGATACTATTGGCCGACCCTCAGCCAAGACGCCAAGAAGTTCCTTAGAACTTGCGACAATTGCCAACGCTACGGAACCATAATCCACCAACCTCCCGAGCTGCTCACCCCCATCTCGGCCCCGTGGCCATTCGCGCAGTGGGGGGTAGATATCATTGGTCCTTTCCCTTTGGGCAAGGGCCAGACCAAGTTCGCTGTGGTTGCTGTGGATTACTTCACCAAGTGGGCCGAGGCCGACGCGCTCTCCCACATAACGGAATCCAGGGTCACGTCCTTCGTATGGACGAATATCATATGTCGCTTTGGTATACCGCAGGCCATAGTGACAGACAATGGGAAGCAGTTTGACAACGCCAAGTTCAAAGACTTTTGCAGCAAACTTGGCATAAGTCATCTTAGCTCGTCCCCCGCACATCCGCAAGCAAATGGCAGATGGAGGCAGTCAACAAGATCATCAAGCGAGGCATCAAACTTAGACTGGACTCCAAGAAAGGCAGGTGGGCCGAGGAGCTACCAGAGGTTTTATGGTCGTACCGGACCACCCAACGAGAGTCGACGGGTGAGACCCCGTTCTCCCTGGCCTTCGGCTCCGAAGCTGTAGTCCCGGTTGAGATTGGCATGCCATCTGACAGAGTAGAGCATTACGAGCCTACGACAAATGAGGAAGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGAAGAGCAATGGCCCAGCTACGCCTGGCGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGAAGGGTCCAAACGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCATTTGAGATCAAGGGCATAGTCCGACCTGGGACGTACATATTGGCCGATCTGAAAGGAGACGTCCTCGCGTACCCGTGGAACGCGGAGCACCTGAAGCGTTATTATCCTTGA

mRNA sequence

ATGGAAATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGTGGACTTCATTAGGGGCAACTCACCACAAGACCCCAAGGAGCGCAGAAAATTGGCAAGGCGGGCAGCTCGGTTCGTGGTCCGAGATGGGGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAGATGCCTAACCCCTGAAGAGGGCCTAGTAGAGCATTACGAGCCTACGACAAATGAGGAAGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGAAGAGCAATGGCCCAGCTACGCCTGGCGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGAAGGGTCCAAACGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCATTTGAGATCAAGGGCATAGTCCGACCTGGGACGTACATATTGGCCGATCTGAAAGGAGACGTCCTCGCGTACCCGTGGAACGCGGAGCACCTGAAGCGTTATTATCCTTGA

Coding sequence (CDS)

ATGGAAATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGTGGACTTCATTAGGGGCAACTCACCACAAGACCCCAAGGAGCGCAGAAAATTGGCAAGGCGGGCAGCTCGGTTCGTGGTCCGAGATGGGGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAGATGCCTAACCCCTGAAGAGGGCCTAGTAGAGCATTACGAGCCTACGACAAATGAGGAAGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGAAGAGCAATGGCCCAGCTACGCCTGGCGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGAAGGGTCCAAACGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCATTTGAGATCAAGGGCATAGTCCGACCTGGGACGTACATATTGGCCGATCTGAAAGGAGACGTCCTCGCGTACCCGTGGAACGCGGAGCACCTGAAGCGTTATTATCCTTGA

Protein sequence

MEIGAPESSWMDPIVDFIRGNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCLTPEEGLVEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDPAWEGPFEIKGIVRPGTYILADLKGDVLAYPWNAEHLKRYYP

Homology

[Close]

BLAST of Moc08g17370 vs. NCBI nr
Match: XP_022159003.1 (uncharacterized protein LOC111025448 [Momordica charantia])

HSP 1 Score: 173.7 bits (439), Expect = 1.4e-39
Identity = 80/111 (72.07%), Postives = 93/111 (83.78%), Query Frame = 0


Query: 66  VEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           +E YEP  NE ELLLNLDLLE +R  AQLRL E Q RMARHYN R++PR+FQVGHLV  +
Sbjct: 519 IEQYEPLRNEAELLLNLDLLEGKRETAQLRLVECQNRMARHYNTRIQPRSFQVGHLVSSK 578

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYILADLKGDVLAYPWNAEHLKRYYP 177
           +Q+HVG LDP+WE  FE+KGIVRPGTY+LADL+  VLA+PWNAEHLKRYYP
Sbjct: 579 IQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc08g17370 vs. NCBI nr
Match: GFY92947.1 (hypothetical protein Acr_08g0013430 [Actinidia rufa])

HSP 1 Score: 157.5 bits (397), Expect = 1.0e-34
Identity = 91/206 (44.17%), Postives = 119/206 (57.77%), Query Frame = 0


Query: 9   SWMDPIVDFIR-GNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCLTPEEG--L 68
           +W+D I+D++R G  P+D  + R+L  R+ARF + +G LY+R FS PLLRCL PEE   +
Sbjct: 200 TWLDEIIDYLRKGILPKDKLQARRLQYRSARFCIFEGRLYKRSFSGPLLRCLRPEEAKYV 259

Query: 69  VEHY---------EP----------------------TTNEEELLLNLDLLEERRAMAQL 128
            E Y         EP                        NE EL LNLDLL+E+R  A+L
Sbjct: 260 YEKYTKAYAGITPEPDRWQRKLFAKDILADHGTRCGYICNEGELRLNLDLLDEKRESAEL 319

Query: 129 RLAEYQGRMARHYNARVRPRAFQVGHLVLRRV-----QTHVGALDPAWEGPFEIKGIVRP 176
           R A Y+GR+A++YN RV  R+F  G LV RRV         G L P WEGP+++  + RP
Sbjct: 320 RQAAYKGRIAKYYNERVEHRSFLPGDLVWRRVTLSTRDPSAGKLGPTWEGPYKVIKVSRP 379

[Close]

BLAST of Moc08g17370 vs. NCBI nr
Match: XP_022156070.1 (uncharacterized protein K02A2.6-like [Momordica charantia])

HSP 1 Score: 151.8 bits (382), Expect = 5.7e-33
Identity = 70/100 (70.00%), Postives = 88/100 (88.00%), Query Frame = 0


Query: 66  VEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           VE+YE   NEEELLL LDL++E+R  AQLRLAEYQ +MARHYN+RVR R+FQ+G+LVLR+
Sbjct: 251 VEYYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARHYNSRVRSRSFQIGYLVLRK 310

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYILADLKGDVLAYP 166
           VQ HV  L+P+WEGPFE+KGIVRPGTY+LADL+G +L++P
Sbjct: 311 VQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc08g17370 vs. NCBI nr
Match: XP_022156070.1 (uncharacterized protein K02A2.6-like [Momordica charantia])

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-07
Identity = 29/54 (53.70%), Postives = 41/54 (75.93%), Query Frame = 0


Query: 11 MDPIVDFIRGNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCLTPEEG 65
          MDPI DF+ G    D KE +++AR+ AR+++R+G LYR GFSLPLL+C+  E+G
Sbjct: 1  MDPIKDFLEGKVSTDLKETKRMARKEARYMLRNGTLYRCGFSLPLLKCIFKEDG 54

HSP 2 Score: 150.2 bits (378), Expect = 1.7e-32
Identity = 90/254 (35.43%), Postives = 125/254 (49.21%), Query Frame = 0


Query: 1   MEIGAPESSWMDPIVDF-IRGNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCL 60
           M++   + +WMD I +F + G+ P DP + RKL  ++A +++++  L++RG+SLPLLRCL
Sbjct: 1   MKVQLAQPTWMDRIKNFLVSGSVPTDPSQARKLRCQSAHYLMQEDKLFKRGYSLPLLRCL 60

Query: 61  TPE-----------------------EGL------------------------------- 120
            PE                        GL                               
Sbjct: 61  DPEVASSSPAHLQSNGQIEAVNKIIKRGLKTRLESLKDRWAEELPEVLWSYRTIPRPSTG 120

Query: 121 ------------------------VEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQG 176
                                   VE++   TN E LL+NLDLL E+R+ +QLRLAEY  
Sbjct: 121 ETPYDLALGSEAVIPVEIGMLTPRVENFNEQTNSEALLVNLDLLAEKRSHSQLRLAEYHN 180

[Close]

BLAST of Moc08g17370 vs. NCBI nr
Match: XP_023876159.1 (uncharacterized protein LOC111988600 [Quercus suber])

HSP 1 Score: 141.7 bits (356), Expect = 5.9e-30
Identity = 78/186 (41.94%), Postives = 108/186 (58.06%), Query Frame = 0


Query: 7    ESSWMDPIVDFIR-GNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCLTPEEG- 66
            E +WM PIV +++ G  P+   E RKL  R+AR+++ D  LY+RGFS P LRCL P+E  
Sbjct: 834  EENWMTPIVSYLKDGRLPEGKDEARKLRVRSARYILMDEVLYKRGFSQPYLRCLAPDEAN 893

Query: 67   --LVEHYEPTTNE--------EELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPR 126
              L E +E              ++ LNLDL++E R  A+ R+A Y+  MAR ++A V+PR
Sbjct: 894  YLLREVHEGACGNHSGARSLVHKIRLNLDLIDEVRMDAEQRVARYKNLMARQHDAMVKPR 953

Query: 127  AFQVGHLVLRRVQTHV-----GALDPAWEGPFEIKGIVRPGTYILADLKGDVLAYPWNAE 176
             F +G LVL++V           L P WEGP+ +    RPG+Y L  L    L +PWN E
Sbjct: 954  RFNIGDLVLKKVSLATKNPAHEKLGPNWEGPYRVINSKRPGSYYLEALDRRKLEHPWNVE 1013

[Close]

BLAST of Moc08g17370 vs. ExPASy TrEMBL
Match: A0A6J1DXL9 (uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025448 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 6.8e-40
Identity = 80/111 (72.07%), Postives = 93/111 (83.78%), Query Frame = 0


Query: 66  VEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           +E YEP  NE ELLLNLDLLE +R  AQLRL E Q RMARHYN R++PR+FQVGHLV  +
Sbjct: 519 IEQYEPLRNEAELLLNLDLLEGKRETAQLRLVECQNRMARHYNTRIQPRSFQVGHLVSSK 578

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYILADLKGDVLAYPWNAEHLKRYYP 177
           +Q+HVG LDP+WE  FE+KGIVRPGTY+LADL+  VLA+PWNAEHLKRYYP
Sbjct: 579 IQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc08g17370 vs. ExPASy TrEMBL
Match: A0A7J0F4U0 (Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_08g0013430 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 5.0e-35
Identity = 91/206 (44.17%), Postives = 119/206 (57.77%), Query Frame = 0


Query: 9   SWMDPIVDFIR-GNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCLTPEEG--L 68
           +W+D I+D++R G  P+D  + R+L  R+ARF + +G LY+R FS PLLRCL PEE   +
Sbjct: 200 TWLDEIIDYLRKGILPKDKLQARRLQYRSARFCIFEGRLYKRSFSGPLLRCLRPEEAKYV 259

Query: 69  VEHY---------EP----------------------TTNEEELLLNLDLLEERRAMAQL 128
            E Y         EP                        NE EL LNLDLL+E+R  A+L
Sbjct: 260 YEKYTKAYAGITPEPDRWQRKLFAKDILADHGTRCGYICNEGELRLNLDLLDEKRESAEL 319

Query: 129 RLAEYQGRMARHYNARVRPRAFQVGHLVLRRV-----QTHVGALDPAWEGPFEIKGIVRP 176
           R A Y+GR+A++YN RV  R+F  G LV RRV         G L P WEGP+++  + RP
Sbjct: 320 RQAAYKGRIAKYYNERVEHRSFLPGDLVWRRVTLSTRDPSAGKLGPTWEGPYKVIKVSRP 379

[Close]

BLAST of Moc08g17370 vs. ExPASy TrEMBL
Match: A0A6J1DP99 (uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023037 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.8e-33
Identity = 70/100 (70.00%), Postives = 88/100 (88.00%), Query Frame = 0


Query: 66  VEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           VE+YE   NEEELLL LDL++E+R  AQLRLAEYQ +MARHYN+RVR R+FQ+G+LVLR+
Sbjct: 251 VEYYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARHYNSRVRSRSFQIGYLVLRK 310

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYILADLKGDVLAYP 166
           VQ HV  L+P+WEGPFE+KGIVRPGTY+LADL+G +L++P
Sbjct: 311 VQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc08g17370 vs. ExPASy TrEMBL
Match: A0A6J1DP99 (uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023037 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 8.9e-08
Identity = 29/54 (53.70%), Postives = 41/54 (75.93%), Query Frame = 0


Query: 11 MDPIVDFIRGNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCLTPEEG 65
          MDPI DF+ G    D KE +++AR+ AR+++R+G LYR GFSLPLL+C+  E+G
Sbjct: 1  MDPIKDFLEGKVSTDLKETKRMARKEARYMLRNGTLYRCGFSLPLLKCIFKEDG 54

HSP 2 Score: 150.2 bits (378), Expect = 8.0e-33
Identity = 90/254 (35.43%), Postives = 125/254 (49.21%), Query Frame = 0


Query: 1   MEIGAPESSWMDPIVDF-IRGNSPQDPKERRKLARRAARFVVRDGALYRRGFSLPLLRCL 60
           M++   + +WMD I +F + G+ P DP + RKL  ++A +++++  L++RG+SLPLLRCL
Sbjct: 1   MKVQLAQPTWMDRIKNFLVSGSVPTDPSQARKLRCQSAHYLMQEDKLFKRGYSLPLLRCL 60

Query: 61  TPE-----------------------EGL------------------------------- 120
            PE                        GL                               
Sbjct: 61  DPEVASSSPAHLQSNGQIEAVNKIIKRGLKTRLESLKDRWAEELPEVLWSYRTIPRPSTG 120

Query: 121 ------------------------VEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQG 176
                                   VE++   TN E LL+NLDLL E+R+ +QLRLAEY  
Sbjct: 121 ETPYDLALGSEAVIPVEIGMLTPRVENFNEQTNSEALLVNLDLLAEKRSHSQLRLAEYHN 180

[Close]

BLAST of Moc08g17370 vs. ExPASy TrEMBL
Match: A0A6J1DWM2 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 3.7e-30
Identity = 65/110 (59.09%), Postives = 85/110 (77.27%), Query Frame = 0


Query: 66  VEHYEPTTNEEELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           VE+++   N E LL+NLDLLEE+R+ +QLRLAEYQ RMAR+YNARV+ R F+   LVL++
Sbjct: 735 VENFDEQANSEALLVNLDLLEEKRSHSQLRLAEYQNRMARYYNARVKVRTFKPSDLVLKK 794

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYILADLKGDVLAYPWNAEHLKRYY 176
           V  HVGAL+P WEGP+ +  I+RP  Y+L+DL G  L +PWNAEHL+ YY
Sbjct: 795 VMQHVGALEPNWEGPYRVLKILRPRAYLLSDLNGRQLPHPWNAEHLRVYY 844

The following BLAST results are available for this feature:

BLAST of Moc08g17370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159003.1	1.4e-39	72.07	uncharacterized protein LOC111025448 [Momordica charantia]	[more]
GFY92947.1	1.0e-34	44.17	hypothetical protein Acr_08g0013430 [Actinidia rufa]	[more]
XP_022156070.1	5.7e-33	70.00	uncharacterized protein K02A2.6-like [Momordica charantia]	[more]
XP_022156070.1	1.8e-07	53.70	uncharacterized protein K02A2.6-like [Momordica charantia]	[more]
XP_023876159.1	5.9e-30	41.94	uncharacterized protein LOC111988600 [Quercus suber]	[more]

BLAST of Moc08g17370 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc08g17370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DXL9	6.8e-40	72.07	uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A7J0F4U0	5.0e-35	44.17	Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_08g0013430 PE=4 SV=1	[more]
A0A6J1DP99	2.8e-33	70.00	uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DP99	8.9e-08	53.70	uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DWM2	3.7e-30	59.09	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1	[more]

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BLAST of Moc08g17370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 75..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g17370.1	Moc08g17370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity