Moc08g16060 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g16060
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	alpha-dioxygenase 1-like
Location	chr8: 12313362 .. 12313781 (+)
RNA-Seq Expression	Moc08g16060
Synteny	Moc08g16060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCACCAAGCAAGTGGGGCCCTCGAGCTTTGGAATTATCCACTGTGGCTTAGGGATCTCATACCATATGATGTGGACGGTAAGGATAGGCCCCATTACGTTGACCTTGCAGCTCTCGAAGGTTGTTGACTCAATCACAACCCTTCATTTCCCTTGATCCAATCTAATCTGCGCCGTTTATTTACATCATGATGGGTAAAAATGTACTTCTGCAGTTTATAGAGACAGAGAGAGGAAGGTTGCTAGGTACAACGACTTCCGCAGGGATTATTGCTCATCCCGATCTCCAAGTGGAAAGATCTAACGGACAATGATGAGGCAATCGAAGTGCTTCGTGACGTGTACGGTGAGGATGTGGAGGAGCTTGATATTTTGGTGGGGTTGATGGGAGAAGAAAATCAAGGGCTTTGCCATTAG

mRNA sequence

ATGGGCCACCAAGCAAGTGGGGCCCTCGAGCTTTGGAATTATCCACTGTGGCTTAGGGATCTCATACCATATGATGTGGACGGTAAGGATAGGCCCCATTACGTTGACCTTGCAGCTCTCGAAGATCTAACGGACAATGATGAGGCAATCGAAGTGCTTCGTGACGTGTACGGTGAGGATGTGGAGGAGCTTGATATTTTGGTGGGGTTGATGGGAGAAGAAAATCAAGGGCTTTGCCATTAG

Coding sequence (CDS)

ATGGGCCACCAAGCAAGTGGGGCCCTCGAGCTTTGGAATTATCCACTGTGGCTTAGGGATCTCATACCATATGATGTGGACGGTAAGGATAGGCCCCATTACGTTGACCTTGCAGCTCTCGAAGATCTAACGGACAATGATGAGGCAATCGAAGTGCTTCGTGACGTGTACGGTGAGGATGTGGAGGAGCTTGATATTTTGGTGGGGTTGATGGGAGAAGAAAATCAAGGGCTTTGCCATTAG

Protein sequence

MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAALEDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEENQGLCH

Homology

[Close]

BLAST of Moc08g16060 vs. NCBI nr
Match: XP_022150126.1 (alpha-dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 129.8 bits (325), Expect = 1.1e-26
Identity = 68/102 (66.67%), Postives = 73/102 (71.57%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDLIP+DVDGKDRP++VDLAAL                    
Sbjct: 418 MGHQASGALELWNYPLWLRDLIPHDVDGKDRPNHVDLAALEVYRDRERKVARYNDFRRGL 477

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD+DEAIEVLRDVYGEDVEELDILVGLM E+
Sbjct: 478 FLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEK 519

[Close]

BLAST of Moc08g16060 vs. NCBI nr
Match: XP_022150068.1 (alpha-dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 127.1 bits (318), Expect = 6.8e-26
Identity = 67/102 (65.69%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDL P+DVDGKDRP +VDLAAL                    
Sbjct: 442 MGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAALEVYRDRERKVARYNDFRRGL 501

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD+DEAIEVLRDVYGEDVEELDILVGLM E+
Sbjct: 502 LLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEK 543

[Close]

BLAST of Moc08g16060 vs. NCBI nr
Match: XP_038903383.1 (alpha-dioxygenase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 120.6 bits (301), Expect = 6.4e-24
Identity = 63/102 (61.76%), Postives = 70/102 (68.63%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQ+SGALELWNYP+WLRDLI +D+DGKDRP +VDLAAL                    
Sbjct: 446 MGHQSSGALELWNYPVWLRDLIAHDMDGKDRPDHVDLAALEVYRDRERRVARYNDFRRGL 505

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTDN EAIEVLRDVYG+DVEELDILVGLM E+
Sbjct: 506 FLIPISKWEDLTDNKEAIEVLRDVYGDDVEELDILVGLMAEK 547

[Close]

BLAST of Moc08g16060 vs. NCBI nr
Match: XP_008443484.1 (PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 120.6 bits (301), Expect = 6.4e-24
Identity = 63/102 (61.76%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDLI +D+DGKDRP +VDLAAL                    
Sbjct: 447 MGHQASGALELWNYPLWLRDLIAHDMDGKDRPDHVDLAALEVYRDRERRVARYNDFRRGL 506

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD++EAIEVLR+VYG+DVEELDILVGLM E+
Sbjct: 507 FLIPISKWEDLTDDEEAIEVLREVYGDDVEELDILVGLMAEK 548

[Close]

BLAST of Moc08g16060 vs. NCBI nr
Match: TYK13980.1 (alpha-dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 120.6 bits (301), Expect = 6.4e-24
Identity = 63/102 (61.76%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDLI +D+DGKDRP +VDLAAL                    
Sbjct: 447 MGHQASGALELWNYPLWLRDLIAHDMDGKDRPDHVDLAALEVYRDRERRVARYNDFRRGL 506

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD++EAIEVLR+VYG+DVEELDILVGLM E+
Sbjct: 507 FLIPISKWEDLTDDEEAIEVLREVYGDDVEELDILVGLMAEK 548

[Close]

BLAST of Moc08g16060 vs. ExPASy Swiss-Prot
Match: Q9SGH6 (Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 4.0e-21
Identity = 54/102 (52.94%), Postives = 66/102 (64.71%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALEL NYP+WLRD++P+D +G+ RP +VDLAAL                    
Sbjct: 443 MGHQASGALELMNYPMWLRDIVPHDPNGQARPDHVDLAALEIYRDRERSVPRYNEFRRSM 502

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLT+++EAIEVL DVY  DVEELD+LVGLM E+
Sbjct: 503 FMIPITKWEDLTEDEEAIEVLDDVYDGDVEELDLLVGLMAEK 544

[Close]

BLAST of Moc08g16060 vs. ExPASy Swiss-Prot
Match: Q9C9U3 (Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 6.4e-19
Identity = 46/102 (45.10%), Postives = 64/102 (62.75%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQ+ GAL LWNYP W+R+L+  D+DG+DRPH +D+AAL                    
Sbjct: 435 MGHQSCGALTLWNYPNWMRNLVAQDIDGEDRPHLIDMAALEIYRDRERGVPRYNEFRKNL 494

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   E+LTD++EAI+VLR+VY +D+E+LD+ VGL  E+
Sbjct: 495 LMSPISKWEELTDDEEAIKVLREVYEDDIEKLDLNVGLHAEK 536

[Close]

BLAST of Moc08g16060 vs. ExPASy TrEMBL
Match: A0A6J1DAJ0 (alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018381 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.1e-27
Identity = 68/102 (66.67%), Postives = 73/102 (71.57%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDLIP+DVDGKDRP++VDLAAL                    
Sbjct: 418 MGHQASGALELWNYPLWLRDLIPHDVDGKDRPNHVDLAALEVYRDRERKVARYNDFRRGL 477

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD+DEAIEVLRDVYGEDVEELDILVGLM E+
Sbjct: 478 FLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEK 519

[Close]

BLAST of Moc08g16060 vs. ExPASy TrEMBL
Match: A0A6J1D8G8 (alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.3e-26
Identity = 67/102 (65.69%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDL P+DVDGKDRP +VDLAAL                    
Sbjct: 442 MGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAALEVYRDRERKVARYNDFRRGL 501

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD+DEAIEVLRDVYGEDVEELDILVGLM E+
Sbjct: 502 LLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEK 543

[Close]

BLAST of Moc08g16060 vs. ExPASy TrEMBL
Match: A0A5A7UJ02 (Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00690 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.1e-24
Identity = 63/102 (61.76%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDLI +D+DGKDRP +VDLAAL                    
Sbjct: 447 MGHQASGALELWNYPLWLRDLIAHDMDGKDRPDHVDLAALEVYRDRERRVARYNDFRRGL 506

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD++EAIEVLR+VYG+DVEELDILVGLM E+
Sbjct: 507 FLIPISKWEDLTDDEEAIEVLREVYGDDVEELDILVGLMAEK 548

[Close]

BLAST of Moc08g16060 vs. ExPASy TrEMBL
Match: A0A5D3CRF7 (Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119G00040 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.1e-24
Identity = 63/102 (61.76%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDLI +D+DGKDRP +VDLAAL                    
Sbjct: 447 MGHQASGALELWNYPLWLRDLIAHDMDGKDRPDHVDLAALEVYRDRERRVARYNDFRRGL 506

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD++EAIEVLR+VYG+DVEELDILVGLM E+
Sbjct: 507 FLIPISKWEDLTDDEEAIEVLREVYGDDVEELDILVGLMAEK 548

[Close]

BLAST of Moc08g16060 vs. ExPASy TrEMBL
Match: A0A1S3B8X6 (alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.1e-24
Identity = 63/102 (61.76%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALELWNYPLWLRDLI +D+DGKDRP +VDLAAL                    
Sbjct: 447 MGHQASGALELWNYPLWLRDLIAHDMDGKDRPDHVDLAALEVYRDRERRVARYNDFRRGL 506

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLTD++EAIEVLR+VYG+DVEELDILVGLM E+
Sbjct: 507 FLIPISKWEDLTDDEEAIEVLREVYGDDVEELDILVGLMAEK 548

[Close]

BLAST of Moc08g16060 vs. TAIR 10
Match: AT3G01420.1 (Peroxidase superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 2.9e-22
Identity = 54/102 (52.94%), Postives = 66/102 (64.71%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQASGALEL NYP+WLRD++P+D +G+ RP +VDLAAL                    
Sbjct: 443 MGHQASGALELMNYPMWLRDIVPHDPNGQARPDHVDLAALEIYRDRERSVPRYNEFRRSM 502

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   EDLT+++EAIEVL DVY  DVEELD+LVGLM E+
Sbjct: 503 FMIPITKWEDLTEDEEAIEVLDDVYDGDVEELDLLVGLMAEK 544

[Close]

BLAST of Moc08g16060 vs. TAIR 10
Match: AT1G73680.1 (alpha dioxygenase )

HSP 1 Score: 94.4 bits (233), Expect = 4.6e-20
Identity = 46/102 (45.10%), Postives = 64/102 (62.75%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQ+ GAL LWNYP W+R+L+  D+DG+DRPH +D+AAL                    
Sbjct: 435 MGHQSCGALTLWNYPNWMRNLVAQDIDGEDRPHLIDMAALEIYRDRERGVPRYNEFRKNL 494

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   E+LTD++EAI+VLR+VY +D+E+LD+ VGL  E+
Sbjct: 495 LMSPISKWEELTDDEEAIKVLREVYEDDIEKLDLNVGLHAEK 536

[Close]

BLAST of Moc08g16060 vs. TAIR 10
Match: AT1G73680.2 (alpha dioxygenase )

HSP 1 Score: 94.4 bits (233), Expect = 4.6e-20
Identity = 46/102 (45.10%), Postives = 64/102 (62.75%), Query Frame = 0


Query: 1   MGHQASGALELWNYPLWLRDLIPYDVDGKDRPHYVDLAAL-------------------- 60
           MGHQ+ GAL LWNYP W+R+L+  D+DG+DRPH +D+AAL                    
Sbjct: 444 MGHQSCGALTLWNYPNWMRNLVAQDIDGEDRPHLIDMAALEIYRDRERGVPRYNEFRKNL 503

Query: 61  --------EDLTDNDEAIEVLRDVYGEDVEELDILVGLMGEE 75
                   E+LTD++EAI+VLR+VY +D+E+LD+ VGL  E+
Sbjct: 504 LMSPISKWEELTDDEEAIKVLREVYEDDIEKLDLNVGLHAEK 545

The following BLAST results are available for this feature:

BLAST of Moc08g16060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150126.1	1.1e-26	66.67	alpha-dioxygenase 1-like [Momordica charantia]	[more]
XP_022150068.1	6.8e-26	65.69	alpha-dioxygenase 1-like [Momordica charantia]	[more]
XP_038903383.1	6.4e-24	61.76	alpha-dioxygenase 1-like isoform X2 [Benincasa hispida]	[more]
XP_008443484.1	6.4e-24	61.76	PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 ...	[more]
TYK13980.1	6.4e-24	61.76	alpha-dioxygenase 1 [Cucumis melo var. makuwa]	[more]

BLAST of Moc08g16060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGH6	4.0e-21	52.94	Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1	[more]
Q9C9U3	6.4e-19	45.10	Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1	[more]

BLAST of Moc08g16060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAJ0	5.1e-27	66.67	alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018381 PE=4 SV=...	[more]
A0A6J1D8G8	3.3e-26	65.69	alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=...	[more]
A0A5A7UJ02	3.1e-24	61.76	Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G...	[more]
A0A5D3CRF7	3.1e-24	61.76	Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119...	[more]
A0A1S3B8X6	3.1e-24	61.76	alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1	[more]

BLAST of Moc08g16060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01420.1	2.9e-22	52.94	Peroxidase superfamily protein	[more]
AT1G73680.1	4.6e-20	45.10	alpha dioxygenase	[more]
AT1G73680.2	4.6e-20	45.10	alpha dioxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037120	Haem peroxidase domain superfamily, animal type	GENE3D	1.10.640.10	Haem peroxidase domain superfamily, animal type	coord: 1..79 e-value: 5.6E-9 score: 36.8
None	No IPR available	PANTHER	PTHR11903	PROSTAGLANDIN G/H SYNTHASE	coord: 1..41
None	No IPR available	PANTHER	PTHR11903:SF32	ALPHA-DIOXYGENASE 1	coord: 41..73
None	No IPR available	PANTHER	PTHR11903	PROSTAGLANDIN G/H SYNTHASE	coord: 41..73
None	No IPR available	PANTHER	PTHR11903:SF32	ALPHA-DIOXYGENASE 1	coord: 1..41
IPR019791	Haem peroxidase, animal-type	PROSITE	PS50292	PEROXIDASE_3	coord: 1..80 score: 9.96328
IPR010255	Haem peroxidase superfamily	SUPERFAMILY	48113	Heme-dependent peroxidases	coord: 1..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g16060.1	Moc08g16060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098869	cellular oxidant detoxification
biological_process	GO:0006979	response to oxidative stress
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0004601	peroxidase activity