Moc08g14820 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc08g14820 vs. NCBI nr
Match: KAG7584790.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 135.6 bits (340), Expect = 5.8e-28
Identity = 89/238 (37.39%), Postives = 127/238 (53.36%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           MA ++W+ +D Q +  IR++LS NV   V KE T + L+KVL D YEKPSAN K     +
Sbjct: 46  MAQEEWDLLDRQVLGVIRLTLSKNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKK 105

Query: 61  YFNIHMEETS-VNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            F++ MEE   V +H+NE   I+N+L  + ++ D++V+A+  L SLP+SWE M+  VSNS
Sbjct: 106 LFHLKMEEGGPVATHVNEFNTIVNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNS 165

Query: 121 LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGKAKMSYN----G 180
           +G   LKF    D  + EE +R            E  + SA   EN+G+ +   N     
Sbjct: 166 VGNQKLKFVDVRDRILGEEVRR--------IDTGETSMSSAFNVENRGRDQNRRNQNRGR 225

Query: 181 KQQRRYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDC----EKRNN---STNVVTEE 227
            + R  +G   S   VEC+ C K GH       FK +C    +K NN     N VT+E
Sbjct: 226 SKSRNGKGQSKSRKPVECWNCGKTGH-------FKSNCWAPPKKENNKGGGANAVTDE 268

BLAST of Moc08g14820 vs. NCBI nr
Match: KAG7561662.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-27
Identity = 88/238 (36.97%), Postives = 126/238 (52.94%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           MA ++W+ +D Q +  IR++LS NV   V KE T + L+KVL D YEKPSAN K     +
Sbjct: 46  MAQEEWDLLDRQVLGVIRLTLSKNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKK 105

Query: 61  YFNIHMEETS-VNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            F++ MEE   V +H+NE   I+N+L  + ++ D++V+A+  + SLP+SWE M+  VSNS
Sbjct: 106 LFHLKMEEGGPVATHVNEFNTIVNQLSSVEIEFDDEVRALILIASLPNSWEPMRAAVSNS 165

Query: 121 LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGKAKMSYN----G 180
           +G   LKF    D  + EE +R            E    SA   EN+G+ +   N     
Sbjct: 166 VGNQKLKFVDVRDRILGEEVRR--------IDTGETSTSSAFNVENRGRDQNRRNQNRGR 225

Query: 181 KQQRRYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDC----EKRNN---STNVVTEE 227
            + R  +G   S   VEC+ C K GH       FK +C    +K NN     N VT+E
Sbjct: 226 SKSRNGKGQSKSRKPVECWNCGKTGH-------FKSNCWAPPKKENNKGGGANAVTDE 268

BLAST of Moc08g14820 vs. NCBI nr
Match: RVW25839.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 133.7 bits (335), Expect = 2.2e-27
Identity = 85/228 (37.28%), Postives = 127/228 (55.70%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           M  ++W  +D Q +  IR++LS +V   V KE T  DL+K L   YEKPSAN K     +
Sbjct: 47  MKAEEWALLDRQVLGVIRLTLSRSVAHNVVKEKTTADLMKALSGMYEKPSANNKVHLMKK 106

Query: 61  YFNIHM-EETSVNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            FN+ M E  SV  H+NE   I N+L  + +  D++++A+  L SLP+SWE M+  VSNS
Sbjct: 107 LFNLKMAENASVAQHLNEFNTITNQLSSVEIDFDDEIRALIVLASLPNSWEAMRMAVSNS 166

Query: 121 LGENSLKFTATCDAAISEEAQRK-LGKMFASTSGAENEVESALVAENKGKAKMSYNGKQQ 180
           +G+  LK+    D  ++EE +R+  G+   S SG+   +E+     NK   +   N +  
Sbjct: 167 IGKEKLKYNDIRDLILAEEIRRRDAGE--TSGSGSALNLETRGRGNNKNSNQGRSNSRNS 226

Query: 181 RRYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDCEKRNNSTNVVTEE 227
            R R    SS +V+C+ C K GH KR  +  K+  +  ++S N VTEE
Sbjct: 227 NRNRSKSRSSQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSANAVTEE 270

BLAST of Moc08g14820 vs. NCBI nr
Match: KAE8691424.1 (Callose synthase 12 [Hibiscus syriacus])

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-27
Identity = 87/229 (37.99%), Postives = 122/229 (53.28%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           M D+DW  +D QA+  IR++LS NV   + KE T   L+  L   YEKPSA+ K     R
Sbjct: 46  MKDEDWALLDRQALGVIRLTLSRNVAFNIAKEKTTAGLMAALSSMYEKPSASNKVHLMRR 105

Query: 61  YFNIHMEE-TSVNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            FN+ M E  SV  H+NEL  I  +L  + ++ D++V+A+  L+SLPDSW    T VS+S
Sbjct: 106 LFNLRMTEGASVAQHLNELNTITTQLSSVEIEFDDEVRALILLSSLPDSWNATVTAVSSS 165

Query: 121 LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGKA--KMSYNGKQ 180
            G + LKF    D  +SEE +R+           E    SAL  E++G+   + S  G+ 
Sbjct: 166 SGNSKLKFDDVRDLVLSEEIRRR--------ESGEASTSSALHTESRGRTSERNSNRGRS 225

Query: 181 QRRYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDCEKRNNSTNVVTEE 227
           + R   S     +  CY C KKGH KR  R  K+D   + ++   VTEE
Sbjct: 226 KSRREKSRTGKKDFTCYNCGKKGHFKRDCRALKKDTGAQESAN--VTEE 264

BLAST of Moc08g14820 vs. NCBI nr
Match: KAG7593230.1 (Pentatricopeptide repeat [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-27
Identity = 80/208 (38.46%), Postives = 114/208 (54.81%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           MA ++W+ +D Q +  IR++LS NV   V KE T + L+KVL D YEKPSAN K     +
Sbjct: 46  MAQEEWDLLDRQVLGVIRLTLSKNVAHNVAKEKTTEGLMKVLSDMYEKPSANNKVFLMKK 105

Query: 61  YFNIHMEETS-VNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            F++ MEE   V +H+NE   I+N+L  + ++ D++V+A+  L SLP+SWE M+  VSNS
Sbjct: 106 LFHLKMEEGGPVATHVNEFNTIVNQLSSVEIEFDDEVRALILLASLPNSWEPMRAAVSNS 165

Query: 121 LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGKAKMSYN----G 180
           +G   LKF    D  + EE +R            E    SA   EN+G+ +   N     
Sbjct: 166 VGNQKLKFVDVRDRILGEEVRR--------IDTGETSTSSAFNVENRGRDQNRRNQNRGR 225

Query: 181 KQQRRYRGSGCSSGEVECYYCHKKGHVK 204
            + R  +G   S   VEC+ C K GH K
Sbjct: 226 SKSRNGKGQSKSRKPVECWNCGKTGHFK 245

BLAST of Moc08g14820 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.4e-15
Identity = 69/247 (27.94%), Postives = 119/247 (48.18%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           M  +DW ++DE+A + IR+ LS +V + +  E TA+ +   L+  Y   +   K     +
Sbjct: 47  MKAEDWADLDERAASAIRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQ 106

Query: 61  YFNIHMEE-TSVNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            + +HM E T+  SH+N    ++ +L  +GVKI+E+ KA+  L SLP S++ + T + + 
Sbjct: 107 LYALHMSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILH- 166

Query: 121 LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGKA--KMSYN-GK 180
            G+ +++      A +  E  RK           EN+ + AL+ E +G++  + S N G+
Sbjct: 167 -GKTTIELKDVTSALLLNEKMRK---------KPENQGQ-ALITEGRGRSYQRSSNNYGR 226

Query: 181 QQRRYRGSGCSSGEV-ECYYCHKKGHVKRFYRKFKEDCEKRNNSTNVVTEEERIDEFLAC 240
              R +    S   V  CY C++ GH       FK DC          + ++  D   A 
Sbjct: 227 SGARGKSKNRSKSRVRNCYNCNQPGH-------FKRDCPNPRKGKGETSGQKNDDNTAAM 274

Query: 241 AQRNISV 243
            Q N +V
Sbjct: 287 VQNNDNV 274

BLAST of Moc08g14820 vs. ExPASy TrEMBL
Match: A0A438CRS1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_4222 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.1e-27
Identity = 85/228 (37.28%), Postives = 127/228 (55.70%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           M  ++W  +D Q +  IR++LS +V   V KE T  DL+K L   YEKPSAN K     +
Sbjct: 47  MKAEEWALLDRQVLGVIRLTLSRSVAHNVVKEKTTADLMKALSGMYEKPSANNKVHLMKK 106

Query: 61  YFNIHM-EETSVNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            FN+ M E  SV  H+NE   I N+L  + +  D++++A+  L SLP+SWE M+  VSNS
Sbjct: 107 LFNLKMAENASVAQHLNEFNTITNQLSSVEIDFDDEIRALIVLASLPNSWEAMRMAVSNS 166

Query: 121 LGENSLKFTATCDAAISEEAQRK-LGKMFASTSGAENEVESALVAENKGKAKMSYNGKQQ 180
           +G+  LK+    D  ++EE +R+  G+   S SG+   +E+     NK   +   N +  
Sbjct: 167 IGKEKLKYNDIRDLILAEEIRRRDAGE--TSGSGSALNLETRGRGNNKNSNQGRSNSRNS 226

Query: 181 RRYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDCEKRNNSTNVVTEE 227
            R R    SS +V+C+ C K GH KR  +  K+  +  ++S N VTEE
Sbjct: 227 NRNRSKSRSSQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSANAVTEE 270

BLAST of Moc08g14820 vs. ExPASy TrEMBL
Match: A0A6A2ZHE4 (Callose synthase 12 OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110890pilonHSYRG01352 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.8e-27
Identity = 87/229 (37.99%), Postives = 122/229 (53.28%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           M D+DW  +D QA+  IR++LS NV   + KE T   L+  L   YEKPSA+ K     R
Sbjct: 46  MKDEDWALLDRQALGVIRLTLSRNVAFNIAKEKTTAGLMAALSSMYEKPSASNKVHLMRR 105

Query: 61  YFNIHMEE-TSVNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            FN+ M E  SV  H+NEL  I  +L  + ++ D++V+A+  L+SLPDSW    T VS+S
Sbjct: 106 LFNLRMTEGASVAQHLNELNTITTQLSSVEIEFDDEVRALILLSSLPDSWNATVTAVSSS 165

Query: 121 LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGKA--KMSYNGKQ 180
            G + LKF    D  +SEE +R+           E    SAL  E++G+   + S  G+ 
Sbjct: 166 SGNSKLKFDDVRDLVLSEEIRRR--------ESGEASTSSALHTESRGRTSERNSNRGRS 225

Query: 181 QRRYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDCEKRNNSTNVVTEE 227
           + R   S     +  CY C KKGH KR  R  K+D   + ++   VTEE
Sbjct: 226 KSRREKSRTGKKDFTCYNCGKKGHFKRDCRALKKDTGAQESAN--VTEE 264

BLAST of Moc08g14820 vs. ExPASy TrEMBL
Match: A0A438JLX4 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3232 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.8e-27
Identity = 85/228 (37.28%), Postives = 127/228 (55.70%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           M  ++W  +D Q +  IR++LS +V   V KE T  DL+KVL   YEKPSAN K     +
Sbjct: 47  MKAEEWALLDRQVLGVIRLTLSRSVAHNVVKEKTTTDLMKVLSGMYEKPSANNKVHLMKK 106

Query: 61  YFNIHM-EETSVNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            FN+ M E  SV  H+NE   I N+L  + +  D++++A+  L SLP+SWE M+  VSNS
Sbjct: 107 LFNLKMAENASVAQHLNEFNTITNQLSSVEIDFDDEIRALIVLASLPNSWEAMRMAVSNS 166

Query: 121 LGENSLKFTATCDAAISEEAQRK-LGKMFASTSGAENEVESALVAENKGKAKMSYNGKQQ 180
            G+  LK+    D  ++EE +R+  G+   S SG+   +E+     N+   +   N +  
Sbjct: 167 TGKEKLKYNDIQDLILAEEIRRRDAGE--TSGSGSALNLETRGRGNNRNSNQGRSNSRNS 226

Query: 181 RRYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDCEKRNNSTNVVTEE 227
            R R    S  +V+C+ C K GH KR  +  K+  +  ++S NVVTEE
Sbjct: 227 NRNRSKSRSGQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSANVVTEE 270

BLAST of Moc08g14820 vs. ExPASy TrEMBL
Match: A0A0D3AEM1 (CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.8e-27
Identity = 92/244 (37.70%), Postives = 132/244 (54.10%), Query Frame = 0

Query: 1   MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
           M   +W  +D Q +  IR++LS NV   V KE T + L+KVL D YEKPSAN+K     +
Sbjct: 46  MDQDEWELLDRQVLGVIRLTLSKNVAHNVVKEKTTEGLMKVLSDMYEKPSANSKVFLMKK 105

Query: 61  YFNIHMEETS-VNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
            F++ MEE   V +HINE   I+N+L  + ++ +++V+A+  L SLP+SWE+M+  VSNS
Sbjct: 106 LFHLKMEEGGLVTAHINEFNTIVNQLSSVEIEFEDEVRALILLASLPNSWESMRVAVSNS 165

Query: 121 LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGKAKMSYNGKQQR 180
           +G   LKF    D  ++EE  R++    ASTS A N VE+     N  +   S    + R
Sbjct: 166 VGTQKLKFNDVRDRILAEEV-RRIDSGEASTSSAFN-VENR--GRNPDRNNRSNGRSKSR 225

Query: 181 RYRGSGCSSGEVECYYCHKKGHVKRFYRKFKEDCEKRNNSTNVVTEEERIDEFLACAQRN 240
             RG        EC+ C K GH+K+  R   +  +      N VT E  ID+ L      
Sbjct: 226 NGRGQSKFRQPAECWNCGKIGHIKKNCRAPPKKEDNTRGGANAVTRE--IDDALV----- 278

Query: 241 ISVD 244
           +SVD
Sbjct: 286 VSVD 278

BLAST of Moc08g14820 vs. ExPASy TrEMBL
Match: A0A445FR60 (Nucleolar pre-ribosomal-associated protein 1 isoform B OS=Glycine soja OX=3848 GN=D0Y65_047996 PE=4 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 2.4e-27
Identity = 89/246 (36.18%), Postives = 133/246 (54.07%), Query Frame = 0

Query: 1    MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTLPWTR 60
            M  ++WN +D QA+  IR++L+ NV   +  E T   L+K L D YEKPSA  K     R
Sbjct: 1919 MKQEEWNLLDRQALGVIRLTLAKNVAFNIVNEKTTAGLMKALSDMYEKPSAANKVYLMRR 1978

Query: 61   YFNIHMEE-TSVNSHINELTDILNKLEGMGVKIDEDVKAMRFLTSLPDSWETMKTVVSNS 120
             FN+ M E  SV  HINE   IL +LE + +K +++VKA+  L+SLPDSW    T VS+S
Sbjct: 1979 LFNLKMGECISVTDHINEFNTILAQLESVQIKFEDEVKALILLSSLPDSWAATVTAVSSS 2038

Query: 121  LGENSLKFTATCDAAISEEAQRKLGKMFASTSGAENEVESALVAENKGK-AKMSYNGKQQ 180
              EN+LK +   D  +SE+ +++      S   + +   SAL  E KG+  +   NG+ +
Sbjct: 2039 TRENTLKLSDIRDLILSEDVRKR-----DSGESSSHVSNSALNIEGKGRTTQKGQNGRGR 2098

Query: 181  RRYRGSGCS--SGEVECYYCHKKGHVKRFYRKFKEDCEKRN------NSTNVVTEEERID 237
             + RG G      +V C+ C K+GH    Y+  K++   +N       S N  T+E  +D
Sbjct: 2099 SKSRGKGQRKFQSDVTCWNCDKRGHFSNQYKAPKKNKSHKNKKRDDDESANAATDE--LD 2157

BLAST of Moc08g14820 vs. TAIR 10
Match: AT3G29785.1 (unknown protein; Has 90 Blast hits to 90 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 90; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 45.4 bits (106), Expect = 7.4e-05
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 0

Query: 1  MADKDWNEMDEQAVANIRMSLSMNVCSLVTKETTAKDLLKVLQDRYEKPSANTKTL 57
          M+  DWN +  Q +  IR+++S N+   V KE +   L+KVL D Y+KPS N   +
Sbjct: 41 MSQDDWNILYRQVLDVIRLTISKNIAHNVAKEKSPDGLMKVLSDIYKKPSTNNTVI 96