Moc08g14770 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g14770
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	ADP-ribosylation factor
Location	chr8: 11407699 .. 11408419 (+)
RNA-Seq Expression	Moc08g14770
Synteny	Moc08g14770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTCTGAAAAGATCCGTCCACTTTGGAGGCACTATTTCCAGATTACACAGTGCTATATTTTTGTTGTGGATAGCAATGATAGGGACCGAGTTGTTGAGGCAAGAGATGAGTTGCAAAGGATGTTGAATGAGGTATGTTAGTATCTGTTTATACCTTTCCACTCTACAAGTTAATGCTGTAATATTTATTTTCTATTTATCCTTTGACACTTCACTCTAACTTGATGGTCTATTGTTGTTTGGACTTTGTTCTTACTCTTATCATTATTCATTAATATCCTGGCTTTACTTTTTAGTTTTTGTTGCTTTATCTCTCAAGGGAGAGTGGATAGAGTTTTACAATCATAAGTCATAACAGCTCAACTCATATGAATATGGTTGATGTAGTTTGAACCTTCCAAATGATAAGAGTTTCTTAGGACTCCTTTTGTGGGGACCGGTTTTTCGTATGCCCTTTTATTTTATTTCATGTTTCTTTATGAAAGTTTGGTTTTTCATACAAAAAAAAAGAGAGGAAAATAGTTTTAACCTTCCAAATGATCAGAGTTTCTCAAGTGAAGCATGTTCAGGACAATTTGAGAAATGAGAGATGGGGCAGGGTTGGGATTTGTATATGTCACGGCAGTGGTTGGTTACTGCATTGGCTGCAGTAGTGGAATCAGTGGTAGGAGTTGCAGTGGTGGATGGACTTGTGAAAGCTAAGGCTTCACCTACTTAG

mRNA sequence

ATGGTTTCTGAAAAGATCCGTCCACTTTGGAGGCACTATTTCCAGATTACACAGTGCTATATTTTTGTTGTGGATAGCAATGATAGGGACCGAGTTGTTGAGGCAAGAGATGAGTTGCAAAGGATGTTGAATGAGTGGTTGGTTACTGCATTGGCTGCAGTAGTGGAATCAGTGGTAGGAGTTGCAGTGGTGGATGGACTTGTGAAAGCTAAGGCTTCACCTACTTAG

Coding sequence (CDS)

ATGGTTTCTGAAAAGATCCGTCCACTTTGGAGGCACTATTTCCAGATTACACAGTGCTATATTTTTGTTGTGGATAGCAATGATAGGGACCGAGTTGTTGAGGCAAGAGATGAGTTGCAAAGGATGTTGAATGAGTGGTTGGTTACTGCATTGGCTGCAGTAGTGGAATCAGTGGTAGGAGTTGCAGTGGTGGATGGACTTGTGAAAGCTAAGGCTTCACCTACTTAG

Protein sequence

MVSEKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNEWLVTALAAVVESVVGVAVVDGLVKAKASPT

Homology

[Close]

BLAST of Moc08g14770 vs. NCBI nr
Match: GBG78737.1 (hypothetical protein CBR_g27961 [Chara braunii])

HSP 1 Score: 80.5 bits (197), Expect = 6.9e-12
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDR+VEARDELQRMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELQRMLNE 113

[Close]

BLAST of Moc08g14770 vs. NCBI nr
Match: KAF5455438.1 (hypothetical protein F2P56_025011, partial [Juglans regia])

HSP 1 Score: 80.5 bits (197), Expect = 6.9e-12
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ +IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGFIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. NCBI nr
Match: XP_035551360.1 (ADP-ribosylation factor 2-like [Juglans regia])

HSP 1 Score: 80.5 bits (197), Expect = 6.9e-12
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ +IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGFIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. NCBI nr
Match: KAG1326082.1 (Protein NPG1 [Cocos nucifera])

HSP 1 Score: 80.1 bits (196), Expect = 9.0e-12
Identity = 38/46 (82.61%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNEWLVT 50
           +KIRPLWRHYFQ TQ  IFVVDSNDRDR+VEARDEL RMLNE  VT
Sbjct: 670 DKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEPFVT 715

[Close]

BLAST of Moc08g14770 vs. NCBI nr
Match: XP_017621936.1 (PREDICTED: ADP-ribosylation factor 2-like, partial [Gossypium arboreum])

HSP 1 Score: 80.1 bits (196), Expect = 9.0e-12
Identity = 38/44 (86.36%), Postives = 39/44 (88.64%), Query Frame = 0


Query: 2  VSEKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
          +S KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 1  LSSKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 44

[Close]

BLAST of Moc08g14770 vs. ExPASy Swiss-Prot
Match: P34727 (ADP-ribosylation factor OS=Ajellomyces capsulatus OX=5037 GN=ARF PE=1 SV=3)

HSP 1 Score: 79.3 bits (194), Expect = 2.0e-14
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEAR+ELQRMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGIIFVVDSNDRDRVVEAREELQRMLNE 113

[Close]

BLAST of Moc08g14770 vs. ExPASy Swiss-Prot
Match: Q7RVM2 (ADP-ribosylation factor OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=NCU08340 PE=3 SV=3)

HSP 1 Score: 79.3 bits (194), Expect = 2.0e-14
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEAR+ELQRMLNE
Sbjct: 75  DKIRPLWRHYFQNTQGIIFVVDSNDRDRVVEAREELQRMLNE 116

[Close]

BLAST of Moc08g14770 vs. ExPASy Swiss-Prot
Match: P36397 (ADP-ribosylation factor 1 OS=Arabidopsis thaliana OX=3702 GN=ARF1 PE=1 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 2.6e-14
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. ExPASy Swiss-Prot
Match: O23778 (ADP-ribosylation factor 1 OS=Catharanthus roseus OX=4058 GN=ARF1 PE=2 SV=3)

HSP 1 Score: 79.0 bits (193), Expect = 2.6e-14
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. ExPASy Swiss-Prot
Match: P51822 (ADP-ribosylation factor 1 OS=Daucus carota OX=4039 GN=ARF1 PE=2 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 2.6e-14
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. ExPASy TrEMBL
Match: A0A388L8V7 (Uncharacterized protein OS=Chara braunii OX=69332 GN=CBR_g27961 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.3e-12
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDR+VEARDELQRMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELQRMLNE 113

[Close]

BLAST of Moc08g14770 vs. ExPASy TrEMBL
Match: A0A6P9ERE3 (ADP-ribosylation factor 2-like OS=Juglans regia OX=51240 GN=LOC109004141 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.3e-12
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ +IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGFIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. ExPASy TrEMBL
Match: A0A5D2I8U3 (Uncharacterized protein OS=Gossypium tomentosum OX=34277 GN=ES332_D12G149100v1 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.3e-12
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ +IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGHIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. ExPASy TrEMBL
Match: A0A6A4Q284 (Putative small GTPase superfamily, ARF/SAR type, P-loop containing nucleoside triphosphate hydrolase OS=Lupinus albus OX=3870 GN=Lalb_Chr09g0334941 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.3e-12
Identity = 39/54 (72.22%), Postives = 44/54 (81.48%), Query Frame = 0


Query: 4  EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNEWLVTALAAVVES 58
          +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE ++ +   V E+
Sbjct: 39 DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEGVLFSRIVVTEA 92

[Close]

BLAST of Moc08g14770 vs. ExPASy TrEMBL
Match: A0A2G5F1S0 (Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_00200131v1 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.3e-12
Identity = 37/44 (84.09%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNEWL 48
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE++
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEFM 115

[Close]

BLAST of Moc08g14770 vs. TAIR 10
Match: AT1G70490.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-15
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. TAIR 10
Match: AT1G23490.1 (ADP-ribosylation factor 1 )

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-15
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. TAIR 10
Match: AT1G10630.1 (ADP-ribosylation factor A1F )

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-15
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. TAIR 10
Match: AT1G70490.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-15
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

[Close]

BLAST of Moc08g14770 vs. TAIR 10
Match: AT1G70490.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-15
Identity = 37/42 (88.10%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 4   EKIRPLWRHYFQITQCYIFVVDSNDRDRVVEARDELQRMLNE 46
           +KIRPLWRHYFQ TQ  IFVVDSNDRDRVVEARDEL RMLNE
Sbjct: 72  DKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNE 113

The following BLAST results are available for this feature:

BLAST of Moc08g14770 vs. NCBI nr


Match Name	E-value	Identity	Description
GBG78737.1	6.9e-12	88.10	hypothetical protein CBR_g27961 [Chara braunii]	[more]
KAF5455438.1	6.9e-12	88.10	hypothetical protein F2P56_025011, partial [Juglans regia]	[more]
XP_035551360.1	6.9e-12	88.10	ADP-ribosylation factor 2-like [Juglans regia]	[more]
KAG1326082.1	9.0e-12	82.61	Protein NPG1 [Cocos nucifera]	[more]
XP_017621936.1	9.0e-12	86.36	PREDICTED: ADP-ribosylation factor 2-like, partial [Gossypium arboreum]	[more]

BLAST of Moc08g14770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34727	2.0e-14	88.10	ADP-ribosylation factor OS=Ajellomyces capsulatus OX=5037 GN=ARF PE=1 SV=3	[more]
Q7RVM2	2.0e-14	88.10	ADP-ribosylation factor OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / C...	[more]
P36397	2.6e-14	88.10	ADP-ribosylation factor 1 OS=Arabidopsis thaliana OX=3702 GN=ARF1 PE=1 SV=2	[more]
O23778	2.6e-14	88.10	ADP-ribosylation factor 1 OS=Catharanthus roseus OX=4058 GN=ARF1 PE=2 SV=3	[more]
P51822	2.6e-14	88.10	ADP-ribosylation factor 1 OS=Daucus carota OX=4039 GN=ARF1 PE=2 SV=2	[more]

BLAST of Moc08g14770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A388L8V7	3.3e-12	88.10	Uncharacterized protein OS=Chara braunii OX=69332 GN=CBR_g27961 PE=3 SV=1	[more]
A0A6P9ERE3	3.3e-12	88.10	ADP-ribosylation factor 2-like OS=Juglans regia OX=51240 GN=LOC109004141 PE=3 SV...	[more]
A0A5D2I8U3	4.3e-12	88.10	Uncharacterized protein OS=Gossypium tomentosum OX=34277 GN=ES332_D12G149100v1 P...	[more]
A0A6A4Q284	4.3e-12	72.22	Putative small GTPase superfamily, ARF/SAR type, P-loop containing nucleoside tr...	[more]
A0A2G5F1S0	4.3e-12	84.09	Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_00200131v1 PE=3...	[more]

BLAST of Moc08g14770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70490.1	1.9e-15	88.10	Ras-related small GTP-binding family protein	[more]
AT1G23490.1	1.9e-15	88.10	ADP-ribosylation factor 1	[more]
AT1G10630.1	1.9e-15	88.10	ADP-ribosylation factor A1F	[more]
AT1G70490.3	1.9e-15	88.10	Ras-related small GTP-binding family protein	[more]
AT1G70490.2	1.9e-15	88.10	Ras-related small GTP-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 2..62 e-value: 4.0E-16 score: 60.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 4..47
IPR006689	Small GTPase superfamily, ARF/SAR type	PFAM	PF00025	Arf	coord: 4..49 e-value: 9.9E-14 score: 51.2
None	No IPR available	PANTHER	PTHR11711:SF391	ADP-RIBOSYLATION FACTOR	coord: 4..45
None	No IPR available	PANTHER	PTHR11711	ADP RIBOSYLATION FACTOR-RELATED	coord: 4..45

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g14770.1	Moc08g14770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0030479	actin cortical patch
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003779	actin binding
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding