Moc08g14420 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g14420
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionGDSL esterase/lipase
Locationchr8: 10921623 .. 10925544 (+)
RNA-Seq ExpressionMoc08g14420
SyntenyMoc08g14420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTCCGGCGATGTTAGTGACTGCGATTTTCGTTTTACAGGTTGTGGTTGTGTCTTCCCAGCCGTGTGACTTTCCGGCGATTTTCAACTTCGGCGACTCGAACTCCGACACCGGCGGTATCTCCGCCGCATTCGAGCCGATTCGAGCGCCGTATGGAGAAGGATTCTTCCACAAGCCGTCTGGACGGGACAGCGACGGCCGTCTCATCATCGACTTCATCGGTAAGTGACGTAACATTCCGTCATTCTCCCTCACTTTCCGGCGAAGTTGTTGAAATTCACTTAGGGCTTCTGATTTTGCAGCCGAGATGTTGAGATTGCCGTACCTGAATGCTTACCTGAATTCGCTCGGCGCTAATTTCCGGTACGGCGCCAATTTTGCCACCGGCGGATCGACGGTGAGGAGGCAGAATGAAACCATTTTTGAATATGGAATAAGTCCATTTTCTCTCGACATTCAGGTTATCCAATTTGAACAGTTCAAAGCTCGCTCCATTGATCTCTACAACCAAGGTTTCAAAATGCCATGAATTAAAGCTTCACTTCATCCTAAATTTAGGCCATAAATATCTTCATATTCTTTTCAATTTAATTATAAATCTGGGTCCCTAAACTCTGGCTGCATTTACTCAGCCTTAAGTTTAAAAAAAAAAATCAATTAGGTCAGTAAAGCTTGAATTGTGATTATCATCGATGATAAGTAGATAGTAGAAATGAGTAGATATCTGGATAAGGTGTCGTGCAACTTATTCTCTTTTCGAAACATAGTTGAATTTTTGTAGTTTTAGATTTGTATCTAATAGGTGATTGGCCTATTAAATATTAAATATTTTTTAAAATTCATAGATTTATTAGACACCGAGTGAAAAGTCAAGATCCTAATATTTAGGAAATTGAATTTTGTGCTTACTTATAATTTGACTTAATTTTATTAAATTTTTGAACGTGTCAGTCATTATCCATTTATTTTTTCTCATTATATGTCATGATATGTCAAATAAACTAATGTTTGTCTTGGTGTCACATTGTCATTTTTTTTAGTACAATAAATGGGGCGGAGGATTCATGACCTTTTTAGTTAGAGATAGGTTCCAATTACGAGCTAAGCTCATGTTTACATGTCATATTGTCATTTAAATTTTATAGATCATGTTGTTTTATTTTGCCCAAATTATCTTTTTTCTTCTTCATCTGTATGTCACCATATGTCATATAAGCTAATGTTTGTCCAATGAGCATATTACATCCTTTTCAAAAAAAAAAAAATTAAATAAATAAAAAAAATAAAAAAGCATATTACATCATCATTTAGATGATAGAGTTAACCTACAGAGGAAACAATTCCAAGTTTGCAAATCTAATAGAAAATTTTGTAAGGATCAGAGACTAAATAACACGATCATCAAAGTTCAGATTAAATTCGTAATTTGACGTTTTCTCTTTTTATCTTGATTTATTTTATTTTATTAATTTTGTTGTTGATGATCATTATTAGCCAAGAATCCATCTGAGAGAGACAAACTTCCAAGACCAGAGGATTTCTCCAAAGCACTCTACACATTTGATATTGGGCAGAATGATCTTTCTGTTGGGTTTAGAAAGCTGAGCACTGATCAACTTCATGCTGCATTGCCTGACATTGTGAACCAGTTTGCCTCTGCAGTTCAAGTAACCAAAATTCTCTCCTCTTTCTCACTAAATTGTTTTCATCCCATTCTTATGTCATCTGATACATTTGAATAACATCCATTTGTTGATATCCTGTCTATATTATAGGCTTTTGGCTGTTTAGTTCAGATGTATTTTCTGAATTAGTGGTTTTTGAAAACTACTTTTCCAGTTTACAGAGTTGTTTAAAACTTACTCGAATTTCTTTCAAACAGTCCGTCGTAGAAAGAATTACAACTTCAACTGTGCTGGAAAACAGTCTTTTGCCATAAGAATGCAAGGGAAATGAGTTTTGGAAAAAAATTACTCCAACATGCTTTGAACTTAATATTGACTTTGGTTATAGGTTGGTTTGAATTAGGCTCGTTGGTTAGTTCTGGTTTTTAAAGCCTAAAACCAAATAATAGTCACCCAGTTCATTTTATATAGATTTTTGTTACGATTGCCCCTAACAGCCAGCCCCGTTGGTAACACCTATCAAAGAGGTCCCATGTTTAAACCTTCAGGCGATCTTAATACCAAAAAAATCCTTAATGTCTTCCGGATCCGAACTTTGGGACGGGTGCTGGTGTCCCAGATAAAGGGAAGTAAGCCAAAAGCTAAATAGCTTCAATTCCGGGTTATCAAAATATATATAGATTTTTGTTACGATTCGGTTTGCAAAAGGGTTTGGCAGCTTCTTCTTTTCTTTATTTCCCTAGACTTATATGGCCATCATTTTTGGCCAAAAACCGGTCTGGACTGAAATTAAAACAAGTTTCAAACTAATGAAGATCATAGTTTTGTTTTGTTTTGTTGTTATTTGTGAGAATGTTAGCGAAATTTGGCGGGATTTGATTAATTTCAGAGAATATACAAGCAAGGAGGGAGATCATTCTGGATACACAACACAGGTCCATTTGGTTGCCTTCCAGTGAACCAATTTTACATACTGAACCCTGCCCCTGGCTTCCTTGATGAGCATGGCTGCATCAAGGCGCAAAATGACATGTCTGTGGAGTTCAATAATCAGCTCAAAGCCAGATTGGTCAAGCTTAAGACAGAGCTCCCTGAAGCTGCCATTACCTATGTGGATGCTTATGCTGCCAAGTATGCCCTAATCAGCAATGGCAAAACTCAAGGTCAGTTCCATCCCTTTAATCATCATCACCATCCTCATCAACACTTGAAAGTATTGCATAAATTTTGTCTTGTTTTGTATCGAGGGAATAAAAGATTAAGAGTCCATTGCCTTGAACCGAGAGGATAGGAACGAAAGAGAAGTGTTTCTGTTGGTAGGCTCACCCATCATATTTTCCTTATGATGAACACGTGCTCATATAGGACATGATTGTGGTGTGTAGTTAGGTTAATTAGAACACGTCCTGAACTCTTTTGGAACGAGTTTTTCTTTTTTAGCTTGGGTTATTCTAATGATATTATGAGCGGTTGTCAATCTTGTGCTCGTATAGGACACAATTGTGCACAATCTATGTTGAAGCATCTCAACACTCTTGAAAATGGATGTATGCGATCGAACCCAAACAACTAGAAATAGATTAAAATATAAAAGATAGAAGAATAACTGTCCAGTAATTCGAGGTATTTGGACCCTTTCCTCTCTCGAATATTCTTACTCAAGCCTAAACCACACTAAACAATTGCATACCTCCATCCCTTCGCCCTCATTCTATTGATAACAAATTCTTATCAACCACCTCCCTAACTCATTACTATTATACCTTGATATCCTAATAATATTCCTAATTCTATCCGAATAGCATAAAAGGAAGACCCGAATAGCATAAAAGGAAGACTCTACCCATTACGGAAACAACAAACGAACTCTAAGAGGGTAGGATAGGAAACAGAACCGCCAAACTGTTGGGTGATGGTAGACTATACTCAAATTAGGTAAAAGAACCCTATGCTCCTGGTTAGTTCAGCTACATGTCTTTCTAAGTCCACATATATTCGAAATAACAACCGATTGATATGTATCTCCGAAACCGAACTTTCGCAGGGTTTCCCGACCCGCTCAAGGTGTGCTGTGGGTACCATGTGAGGTATGACCATGTGTGGTGTGGGACAAAAGCAAGCATTAATGGGAGGTTGGTGTATGGAGGTGCCTGTGAAAACAAAGCTCAGTTTGTGAGTTGGGATGGAGTCCATTATTCAGAGGCAGCTAATCACTTTGTTGCCAATCACATTCTCAATGGTTCTCTCTCAACCCCTCCTATTCCAATCACTCAAGCATGCCATAGGAGTTAA

mRNA sequence

ATGGAGTTTCCGGCGATGTTAGTGACTGCGATTTTCGTTTTACAGGTTGTGGTTGTGTCTTCCCAGCCGTGTGACTTTCCGGCGATTTTCAACTTCGGCGACTCGAACTCCGACACCGGCGGTATCTCCGCCGCATTCGAGCCGATTCGAGCGCCGTATGGAGAAGGATTCTTCCACAAGCCGTCTGGACGGGACAGCGACGGCCGTCTCATCATCGACTTCATCGCCGAGATGTTGAGATTGCCGTACCTGAATGCTTACCTGAATTCGCTCGGCGCTAATTTCCGGTACGGCGCCAATTTTGCCACCGGCGGATCGACGGTGAGGAGGCAGAATGAAACCATTTTTGAATATGGAATAAGTCCATTTTCTCTCGACATTCAGGTTATCCAATTTGAACAGTTCAAAGCTCGCTCCATTGATCTCTACAACCAAGCCAAGAATCCATCTGAGAGAGACAAACTTCCAAGACCAGAGGATTTCTCCAAAGCACTCTACACATTTGATATTGGGCAGAATGATCTTTCTGTTGGGTTTAGAAAGCTGAGCACTGATCAACTTCATGCTGCATTGCCTGACATTGTGAACCAGTTTGCCTCTGCAGTTCAAAGAATATACAAGCAAGGAGGGAGATCATTCTGGATACACAACACAGGTCCATTTGGTTGCCTTCCAGTGAACCAATTTTACATACTGAACCCTGCCCCTGGCTTCCTTGATGAGCATGGCTGCATCAAGGCGCAAAATGACATGTCTGTGGAGTTCAATAATCAGCTCAAAGCCAGATTGGTCAAGCTTAAGACAGAGCTCCCTGAAGCTGCCATTACCTATGTGGATGCTTATGCTGCCAAGTATGCCCTAATCAGCAATGGCAAAACTCAAGGGTTTCCCGACCCGCTCAAGGTGTGCTGTGGGTACCATGTGAGGTATGACCATGTGTGGTGTGGGACAAAAGCAAGCATTAATGGGAGGTTGGTGTATGGAGGTGCCTGTGAAAACAAAGCTCAGTTTGTGAGTTGGGATGGAGTCCATTATTCAGAGGCAGCTAATCACTTTGTTGCCAATCACATTCTCAATGGTTCTCTCTCAACCCCTCCTATTCCAATCACTCAAGCATGCCATAGGAGTTAA

Coding sequence (CDS)

ATGGAGTTTCCGGCGATGTTAGTGACTGCGATTTTCGTTTTACAGGTTGTGGTTGTGTCTTCCCAGCCGTGTGACTTTCCGGCGATTTTCAACTTCGGCGACTCGAACTCCGACACCGGCGGTATCTCCGCCGCATTCGAGCCGATTCGAGCGCCGTATGGAGAAGGATTCTTCCACAAGCCGTCTGGACGGGACAGCGACGGCCGTCTCATCATCGACTTCATCGCCGAGATGTTGAGATTGCCGTACCTGAATGCTTACCTGAATTCGCTCGGCGCTAATTTCCGGTACGGCGCCAATTTTGCCACCGGCGGATCGACGGTGAGGAGGCAGAATGAAACCATTTTTGAATATGGAATAAGTCCATTTTCTCTCGACATTCAGGTTATCCAATTTGAACAGTTCAAAGCTCGCTCCATTGATCTCTACAACCAAGCCAAGAATCCATCTGAGAGAGACAAACTTCCAAGACCAGAGGATTTCTCCAAAGCACTCTACACATTTGATATTGGGCAGAATGATCTTTCTGTTGGGTTTAGAAAGCTGAGCACTGATCAACTTCATGCTGCATTGCCTGACATTGTGAACCAGTTTGCCTCTGCAGTTCAAAGAATATACAAGCAAGGAGGGAGATCATTCTGGATACACAACACAGGTCCATTTGGTTGCCTTCCAGTGAACCAATTTTACATACTGAACCCTGCCCCTGGCTTCCTTGATGAGCATGGCTGCATCAAGGCGCAAAATGACATGTCTGTGGAGTTCAATAATCAGCTCAAAGCCAGATTGGTCAAGCTTAAGACAGAGCTCCCTGAAGCTGCCATTACCTATGTGGATGCTTATGCTGCCAAGTATGCCCTAATCAGCAATGGCAAAACTCAAGGGTTTCCCGACCCGCTCAAGGTGTGCTGTGGGTACCATGTGAGGTATGACCATGTGTGGTGTGGGACAAAAGCAAGCATTAATGGGAGGTTGGTGTATGGAGGTGCCTGTGAAAACAAAGCTCAGTTTGTGAGTTGGGATGGAGTCCATTATTCAGAGGCAGCTAATCACTTTGTTGCCAATCACATTCTCAATGGTTCTCTCTCAACCCCTCCTATTCCAATCACTCAAGCATGCCATAGGAGTTAA

Protein sequence

MEFPAMLVTAIFVLQVVVVSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLSTPPIPITQACHRS
Homology
BLAST of Moc08g14420 vs. NCBI nr
Match: XP_022152152.1 (GDSL esterase/lipase At5g14450-like, partial [Momordica charantia])

HSP 1 Score: 691.0 bits (1782), Expect = 5.6e-195
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 49  IRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTV 108
           IRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTV
Sbjct: 1   IRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTV 60

Query: 109 RRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTF 168
           RRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTF
Sbjct: 61  RRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTF 120

Query: 169 DIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQ 228
           DIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQ
Sbjct: 121 DIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQ 180

Query: 229 FYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALI 288
           FYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALI
Sbjct: 181 FYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALI 240

Query: 289 SNGKTQGFPDPLKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEA 348
           SNGKTQGFPDPLKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEA
Sbjct: 241 SNGKTQGFPDPLKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEA 300

Query: 349 ANHFVANHILNGSLSTPPIPITQACHRS 377
           ANHFVANHILNGSLSTPPIPITQACHRS
Sbjct: 301 ANHFVANHILNGSLSTPPIPITQACHRS 328

BLAST of Moc08g14420 vs. NCBI nr
Match: KAG6581060.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 320/377 (84.88%), Postives = 346/377 (91.78%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFH 60
           MEFPAM+V A  VLQV    + P CDFP +FNFGDSNSDTG ISAAFEPIR PYG+ FF+
Sbjct: 1   MEFPAMVVFAFLVLQVTFALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFN 60

Query: 61  KPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYG 120
           KPSGRDSDGRLI+DFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTVR+ NETI+EYG
Sbjct: 61  KPSGRDSDGRLIVDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYG 120

Query: 121 ISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGF 180
           ISPF LD+QV QFEQFKARS DLYNQAKNPS+R+KL RPEDFSKALYTFDIGQNDL++GF
Sbjct: 121 ISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGF 180

Query: 181 RKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFL 240
           RKLS DQL AALPDIVNQFASAVQR+YK GGRSFWIHNTGPFGCLPVNQFY LNPAPG L
Sbjct: 181 RKLSIDQLRAALPDIVNQFASAVQRLYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAPGIL 240

Query: 241 DEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDP 300
           DEHGCIKAQND+S+E NNQLKA+L KL+ ELPEAAITYVD YAAKYALISNGKTQGFP+P
Sbjct: 241 DEHGCIKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGFPNP 300

Query: 301 LKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILN 360
           LKVCCGYHVRYDHVWCGTKA ING LV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILN
Sbjct: 301 LKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILN 360

Query: 361 GSLSTPPIPITQACHRS 377
           GSLS+PPIPIT+ACHRS
Sbjct: 361 GSLSSPPIPITKACHRS 377

BLAST of Moc08g14420 vs. NCBI nr
Match: XP_022982673.1 (GDSL esterase/lipase At5g14450-like [Cucurbita maxima])

HSP 1 Score: 674.5 bits (1739), Expect = 5.4e-190
Identity = 320/377 (84.88%), Postives = 346/377 (91.78%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFH 60
           MEFPAM+V A  VLQV    + P CDFP +FNFGDSNSDTG ISAAFEPIR PYG+ FF+
Sbjct: 1   MEFPAMVVFAFLVLQVTFALALPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFN 60

Query: 61  KPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYG 120
           KPSGRDSDGRLIIDFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTVR+ NETI+EYG
Sbjct: 61  KPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYG 120

Query: 121 ISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGF 180
           ISPF LD+QV QFEQFKARS DLYNQAKNPS+R+KL RPEDFSKALYTFDIGQNDL++GF
Sbjct: 121 ISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGF 180

Query: 181 RKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFL 240
           RKLS DQL AALP+IVNQFASAVQRIYK GGRSFWIHNTGPFGCLPVNQFY LNPAPG L
Sbjct: 181 RKLSIDQLRAALPEIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAPGIL 240

Query: 241 DEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDP 300
           DEHGCIKAQND+S+E NNQLKA+L KL+ ELP+AAITYVD YAAKYALISNGKTQGFP+P
Sbjct: 241 DEHGCIKAQNDVSMELNNQLKAKLEKLRAELPDAAITYVDVYAAKYALISNGKTQGFPNP 300

Query: 301 LKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILN 360
           LKVCCGYHVRYDHVWCGTKA ING LV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILN
Sbjct: 301 LKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILN 360

Query: 361 GSLSTPPIPITQACHRS 377
           GSLS+PPIPIT+ACHRS
Sbjct: 361 GSLSSPPIPITKACHRS 377

BLAST of Moc08g14420 vs. NCBI nr
Match: XP_023526085.1 (GDSL esterase/lipase At5g14450-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 671.8 bits (1732), Expect = 3.5e-189
Identity = 319/377 (84.62%), Postives = 345/377 (91.51%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFH 60
           MEFPA +V A+ VLQV    + P CDFP +FNFGDSNSDTG ISAAFEPIR PYG+ FF+
Sbjct: 1   MEFPAKVVFALLVLQVTFALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFN 60

Query: 61  KPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYG 120
           KPSGRDSDGRLIIDFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTV++ NETI+EYG
Sbjct: 61  KPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVQKPNETIYEYG 120

Query: 121 ISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGF 180
           ISPF LD+QV QFEQFKARS DLYNQAKNPS+R+KL RPEDFSKALYTFDIGQNDL++GF
Sbjct: 121 ISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGF 180

Query: 181 RKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFL 240
           RKLS DQL AALPDIVNQFASAVQRIYK  GRSFWIHNTGPFGCLPVNQFY LNPAPG L
Sbjct: 181 RKLSIDQLRAALPDIVNQFASAVQRIYKLEGRSFWIHNTGPFGCLPVNQFYTLNPAPGIL 240

Query: 241 DEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDP 300
           DEHGCIKAQND+S+E NNQLKA+L KL+ ELPEAAITYVD YAAKYALISNGKTQGFP+P
Sbjct: 241 DEHGCIKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGFPNP 300

Query: 301 LKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILN 360
           LKVCCGYHVRYDHVWCGTKA ING LV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILN
Sbjct: 301 LKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILN 360

Query: 361 GSLSTPPIPITQACHRS 377
           GSLS+PPIPIT+ACHRS
Sbjct: 361 GSLSSPPIPITKACHRS 377

BLAST of Moc08g14420 vs. NCBI nr
Match: XP_022935096.1 (GDSL esterase/lipase At5g14450-like [Cucurbita moschata])

HSP 1 Score: 667.5 bits (1721), Expect = 6.6e-188
Identity = 317/372 (85.22%), Postives = 341/372 (91.67%), Query Frame = 0

Query: 6   MLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGR 65
           M+V A  VLQV    + P CDFP +FNFGDSNSDTG ISAAFEPIR PYG+ FF+KPSGR
Sbjct: 1   MVVFAFLVLQVTFALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGR 60

Query: 66  DSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFS 125
           DSDGRLIIDFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTVR+ NETI+EYGISPF 
Sbjct: 61  DSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFF 120

Query: 126 LDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLST 185
           LD+QV QFEQFKARS DLYNQAKNPS+R+KL RPEDFSKALYTFDIGQNDL++GFRKLS 
Sbjct: 121 LDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGFRKLSI 180

Query: 186 DQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFLDEHGC 245
           DQL AALPDIVNQFASAVQRIYK GGRSFWIHNTGPFGCLPVNQFY LNPAPG LDEHGC
Sbjct: 181 DQLRAALPDIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAPGILDEHGC 240

Query: 246 IKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCC 305
           IKAQND+S+E NNQLKA+L KL+ ELPEAAITYVD YAAKYALISNGKTQGFP+PLKVCC
Sbjct: 241 IKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGFPNPLKVCC 300

Query: 306 GYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLST 365
           GYHVRYDHVWCGTKA ING LV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILNGSLS+
Sbjct: 301 GYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILNGSLSS 360

Query: 366 PPIPITQACHRS 377
           PPIPIT+ACHRS
Sbjct: 361 PPIPITKACHRS 372

BLAST of Moc08g14420 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 8.2e-157
Identity = 250/369 (67.75%), Postives = 303/369 (82.11%), Query Frame = 0

Query: 7   LVTAIFVLQVVVVSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGRDS 66
           L+  +F +   V     C FPAI+NFGDSNSDTGGISAAFEPIR PYG+GFFH+P+GRDS
Sbjct: 20  LLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDS 79

Query: 67  DGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFSLD 126
           DGRL IDFIAE L LPYL+AYLNSLG+NFR+GANFATGGST+RRQNETIF+YGISPFSLD
Sbjct: 80  DGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLD 139

Query: 127 IQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLSTDQ 186
           +Q+ QF+QFKARS  L+ Q K+  +R+KLPR E+F+KALYTFDIGQNDLSVGFR +S DQ
Sbjct: 140 MQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSVDQ 199

Query: 187 LHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFLDEHGCIK 246
           L A +PDIVN  ASAV+ IY+QGGR+FW+HNTGPFGCLPVN FY+  PAPG+LD+ GC+K
Sbjct: 200 LKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVK 259

Query: 247 AQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGY 306
           AQN+M++EFN +LK  ++ L+ EL +AAITYVD Y AKY ++SN K  GF +PLKVCCGY
Sbjct: 260 AQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGY 319

Query: 307 HVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLSTPP 366
           H +YDH+WCG K  +N   +YGG+C N    VSWDGVHY+EAAN  VA+  LNG L+ PP
Sbjct: 320 HEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPP 379

Query: 367 IPITQACHR 376
           +PIT+AC+R
Sbjct: 380 VPITRACYR 388

BLAST of Moc08g14420 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 4.1e-100
Identity = 184/386 (47.67%), Postives = 258/386 (66.84%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVV---VSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGF 60
           ME   +LV  + +   ++     S  C+FPAIFNFGDSNSDTGG+SA+F     P G+ F
Sbjct: 1   METNLLLVKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTF 60

Query: 61  FHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFE 120
           FH PSGR SDGRLIIDFIAE L LPYLNA+L+S+G+NF +GANFAT GSTVR  N TI +
Sbjct: 61  FHSPSGRFSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQ 120

Query: 121 YGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSV 180
            G+SP SLD+Q++QF  F  RS  + N  +    +  LP+ E FS+ALYTFDIGQNDL+ 
Sbjct: 121 SGVSPISLDVQLVQFSDFITRSQLIRN--RGGVFKKLLPKKEYFSQALYTFDIGQNDLTA 180

Query: 181 GFR-KLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILN--P 240
           G +  +++DQ+ A +PD+ +Q ++ ++++Y +GGR FWIHNT P GCLP    Y+L+  P
Sbjct: 181 GLKLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLP----YVLDRFP 240

Query: 241 APGF-LDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKT 300
            P   +D HGC   +N+++  +N++LK R+++L+ EL EAA TYVD Y+ K  LI+  K 
Sbjct: 241 VPASQIDNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKK 300

Query: 301 QGFPDPLKVCCGYHVRYDH---VWCGTKASINGR-LVYGGACENKAQFVSWDGVHYSEAA 360
            GF  PL  CCG+  +Y+    + CG K  I G+ +V   +C + +  VSWDG+H++E  
Sbjct: 301 LGFRYPLVACCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETT 360

Query: 361 NHFVANHILNGSLSTPPIPITQACHR 376
           N ++   I +G+ S PP+P+  AC R
Sbjct: 361 NSWIFQQINDGAFSDPPLPVKSACTR 380

BLAST of Moc08g14420 vs. ExPASy Swiss-Prot
Match: Q9LII9 (GDSL esterase/lipase At3g27950 OS=Arabidopsis thaliana OX=3702 GN=At3g27950 PE=2 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.3e-98
Identity = 182/370 (49.19%), Postives = 238/370 (64.32%), Query Frame = 0

Query: 5   AMLVTAIFVLQVVVVSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGR 64
           A++V  +   + +   S  C+FPA+FNFGDSNSDTG ISAA   +  P G  FF + +GR
Sbjct: 9   AIIVLLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGR 68

Query: 65  DSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFS 124
            SDGRLIIDFI E L LPYL  YL+S+GAN+R+GANFATGGS +R           SPF 
Sbjct: 69  HSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPTLAC-----FSPFH 128

Query: 125 LDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLST 184
           L  QV QF  FK R++ LYNQ      R  L     FSKALYT DIGQNDL++GF+ ++ 
Sbjct: 129 LGTQVSQFIHFKTRTLSLYNQTNGKFNR--LSHTNYFSKALYTLDIGQNDLAIGFQNMTE 188

Query: 185 DQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGF-LDEHG 244
           +QL A +P I+  F  A++ +YK+G R F IHNTGP GCLP    Y+L   P    D +G
Sbjct: 189 EQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLP----YLLKAFPAIPRDPYG 248

Query: 245 CIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVC 304
           C+K  N++++EFN QLK ++ +LK ELP +  TYVD Y+AKY LI+  K  GF DP   C
Sbjct: 249 CLKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYC 308

Query: 305 CGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLS 364
           C   +    + CG    +NG  +Y  +C+N+  F+SWDG+HY+E AN  VAN IL+GS+S
Sbjct: 309 CVGAIGRG-MGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSIS 366

Query: 365 TPPIPITQAC 374
            PP+P  +AC
Sbjct: 369 DPPLPTQKAC 366

BLAST of Moc08g14420 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 3.8e-98
Identity = 188/360 (52.22%), Postives = 237/360 (65.83%), Query Frame = 0

Query: 24  CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPY 83
           C FPA+FNFGDSNSDTGG+S+ F     P G  FF  P+GR  DGRL+IDFIAE L L +
Sbjct: 38  CHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGMPAGRYCDGRLVIDFIAESLGLTH 97

Query: 84  LNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLY 143
           L+AYLNS+G+NF  GANFAT GS++RRQN ++F  G SP SLD+Q  +FEQF  RS  +Y
Sbjct: 98  LSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGFSPISLDVQFWEFEQFINRSQLVY 157

Query: 144 NQAKNPSERDKLPRPEDFSKALYTFDIGQNDL-SVGFRKLSTDQLHAALPDIVNQFASAV 203
           N  K    R+ LPR E FS+ALYTFDIGQND+ S  F   +T+++ A +PD++ +  S +
Sbjct: 158 NN-KGGIYREILPRAEYFSQALYTFDIGQNDITSSYFVNNTTEEVEAIIPDLMERLTSII 217

Query: 204 QRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGF---LDEHGCIKAQNDMSVEFNNQL 263
           Q +Y +GGR FWIHNTGP GCLP    Y L   P      D  GC    N ++  FN +L
Sbjct: 218 QSVYSRGGRYFWIHNTGPLGCLP----YALLHRPDLAIPADGTGCSVTYNKVAQLFNLRL 277

Query: 264 KARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGY-HVRYD---HVWC 323
           K  +  L+   P+AA TYVD Y AKY LIS     GF DPL  CCGY   RY+    V C
Sbjct: 278 KETVASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLLTCCGYGGGRYNLDLSVGC 337

Query: 324 GTKASING-RLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLSTPPIPITQACH 375
           G K  +NG  +V G +CEN ++ VSWDGVH++EAAN FV + I+ G+LS PP+ + QACH
Sbjct: 338 GGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQIVAGALSDPPVALRQACH 392

BLAST of Moc08g14420 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 5.2e-95
Identity = 182/388 (46.91%), Postives = 250/388 (64.43%), Query Frame = 0

Query: 1   MEFPAM----LVTAIFVLQV--VVVSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYG 60
           MEFP      ++T  F+L +  +  +S+ CDFPAIFNFGDSNSDTGG +AAF P+  PYG
Sbjct: 1   MEFPETNNNPIITLSFLLCMLSLAYASETCDFPAIFNFGDSNSDTGGKAAAFYPLNPPYG 60

Query: 61  EGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNET 120
           E FFH+ +GR SDGRLIIDFIAE   LPYL+ YL+SLG+NF++GA+FAT GST++     
Sbjct: 61  ETFFHRSTGRYSDGRLIIDFIAESFNLPYLSPYLSSLGSNFKHGADFATAGSTIKLPTTI 120

Query: 121 IFEY-GISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQN 180
           I  + G SPF LD+Q  QF QF  RS   + +       + +P    F KALYTFDIGQN
Sbjct: 121 IPAHGGFSPFYLDVQYSQFRQFIPRS--QFIRETGGIFAELVPEEYYFEKALYTFDIGQN 180

Query: 181 DLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILN 240
           DL+ GF  L+ ++++A +PD+VN F++ V++IY  G R+FWIHNTGP GCL     +IL 
Sbjct: 181 DLTEGFLNLTVEEVNATVPDLVNSFSANVKKIYDLGARTFWIHNTGPIGCLS----FILT 240

Query: 241 PAP-GFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGK 300
             P    D  GC KA N+++  FN++LK  + +L+ +LP A   +VD Y+ KY+L S  +
Sbjct: 241 YFPWAEKDSAGCAKAYNEVAQHFNHKLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPE 300

Query: 301 TQGFPDPLKVCCGYHVRYDH---VWCG-TKASINGRLVYGGACENKAQFVSWDGVHYSEA 360
             GF  PL  CCGY  +Y+      CG T  + +G  +  G+C   +  V+WDG HY+EA
Sbjct: 301 KHGFEFPLITCCGYGGKYNFSVTAPCGDTVTADDGTKIVVGSCACPSVRVNWDGAHYTEA 360

Query: 361 ANHFVANHILNGSLSTPPIPITQACHRS 377
           AN +  + I  G+ S PP+P+  ACH++
Sbjct: 361 ANEYFFDQISTGAFSDPPVPLNMACHKT 382

BLAST of Moc08g14420 vs. ExPASy TrEMBL
Match: A0A6J1DFE7 (GDSL esterase/lipase At5g14450-like OS=Momordica charantia OX=3673 GN=LOC111019941 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.7e-195
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 49  IRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTV 108
           IRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTV
Sbjct: 1   IRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTV 60

Query: 109 RRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTF 168
           RRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTF
Sbjct: 61  RRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTF 120

Query: 169 DIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQ 228
           DIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQ
Sbjct: 121 DIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQ 180

Query: 229 FYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALI 288
           FYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALI
Sbjct: 181 FYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALI 240

Query: 289 SNGKTQGFPDPLKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEA 348
           SNGKTQGFPDPLKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEA
Sbjct: 241 SNGKTQGFPDPLKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEA 300

Query: 349 ANHFVANHILNGSLSTPPIPITQACHRS 377
           ANHFVANHILNGSLSTPPIPITQACHRS
Sbjct: 301 ANHFVANHILNGSLSTPPIPITQACHRS 328

BLAST of Moc08g14420 vs. ExPASy TrEMBL
Match: A0A6J1J5G0 (GDSL esterase/lipase At5g14450-like OS=Cucurbita maxima OX=3661 GN=LOC111481477 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.6e-190
Identity = 320/377 (84.88%), Postives = 346/377 (91.78%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFH 60
           MEFPAM+V A  VLQV    + P CDFP +FNFGDSNSDTG ISAAFEPIR PYG+ FF+
Sbjct: 1   MEFPAMVVFAFLVLQVTFALALPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFN 60

Query: 61  KPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYG 120
           KPSGRDSDGRLIIDFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTVR+ NETI+EYG
Sbjct: 61  KPSGRDSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYG 120

Query: 121 ISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGF 180
           ISPF LD+QV QFEQFKARS DLYNQAKNPS+R+KL RPEDFSKALYTFDIGQNDL++GF
Sbjct: 121 ISPFFLDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGF 180

Query: 181 RKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFL 240
           RKLS DQL AALP+IVNQFASAVQRIYK GGRSFWIHNTGPFGCLPVNQFY LNPAPG L
Sbjct: 181 RKLSIDQLRAALPEIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAPGIL 240

Query: 241 DEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDP 300
           DEHGCIKAQND+S+E NNQLKA+L KL+ ELP+AAITYVD YAAKYALISNGKTQGFP+P
Sbjct: 241 DEHGCIKAQNDVSMELNNQLKAKLEKLRAELPDAAITYVDVYAAKYALISNGKTQGFPNP 300

Query: 301 LKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILN 360
           LKVCCGYHVRYDHVWCGTKA ING LV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILN
Sbjct: 301 LKVCCGYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILN 360

Query: 361 GSLSTPPIPITQACHRS 377
           GSLS+PPIPIT+ACHRS
Sbjct: 361 GSLSSPPIPITKACHRS 377

BLAST of Moc08g14420 vs. ExPASy TrEMBL
Match: A0A6J1F3K8 (GDSL esterase/lipase At5g14450-like OS=Cucurbita moschata OX=3662 GN=LOC111442066 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.2e-188
Identity = 317/372 (85.22%), Postives = 341/372 (91.67%), Query Frame = 0

Query: 6   MLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGR 65
           M+V A  VLQV    + P CDFP +FNFGDSNSDTG ISAAFEPIR PYG+ FF+KPSGR
Sbjct: 1   MVVFAFLVLQVTFALASPDCDFPVVFNFGDSNSDTGAISAAFEPIRWPYGDVFFNKPSGR 60

Query: 66  DSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFS 125
           DSDGRLIIDFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTVR+ NETI+EYGISPF 
Sbjct: 61  DSDGRLIIDFIAERLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYGISPFF 120

Query: 126 LDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLST 185
           LD+QV QFEQFKARS DLYNQAKNPS+R+KL RPEDFSKALYTFDIGQNDL++GFRKLS 
Sbjct: 121 LDMQVTQFEQFKARSNDLYNQAKNPSDREKLTRPEDFSKALYTFDIGQNDLAMGFRKLSI 180

Query: 186 DQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFLDEHGC 245
           DQL AALPDIVNQFASAVQRIYK GGRSFWIHNTGPFGCLPVNQFY LNPAPG LDEHGC
Sbjct: 181 DQLRAALPDIVNQFASAVQRIYKLGGRSFWIHNTGPFGCLPVNQFYTLNPAPGILDEHGC 240

Query: 246 IKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCC 305
           IKAQND+S+E NNQLKA+L KL+ ELPEAAITYVD YAAKYALISNGKTQGFP+PLKVCC
Sbjct: 241 IKAQNDISMELNNQLKAKLEKLRAELPEAAITYVDVYAAKYALISNGKTQGFPNPLKVCC 300

Query: 306 GYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLST 365
           GYHVRYDHVWCGTKA ING LV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILNGSLS+
Sbjct: 301 GYHVRYDHVWCGTKAPINGSLVFGDACENRAQYVSWDGVHYSEAANHFVANHILNGSLSS 360

Query: 366 PPIPITQACHRS 377
           PPIPIT+ACHRS
Sbjct: 361 PPIPITKACHRS 372

BLAST of Moc08g14420 vs. ExPASy TrEMBL
Match: A0A5D3D5A1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00280 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 6.0e-187
Identity = 314/377 (83.29%), Postives = 342/377 (90.72%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFH 60
           MEF A  ++A  V  V V  + P C+FP IFNFGDSNSDTG ISAAFEPIR PYG+ FF+
Sbjct: 1   MEFSATALSAFLVFHVFVALASPDCNFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFN 60

Query: 61  KPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYG 120
           KPSGRDSDGRLIIDFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTVR+ NETI+EYG
Sbjct: 61  KPSGRDSDGRLIIDFIAEKLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYG 120

Query: 121 ISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGF 180
           ISPF LD+QV QFEQFKARS DLYNQAKNP +R+KL RPED+SKALYTFDIGQNDL+VGF
Sbjct: 121 ISPFFLDMQVTQFEQFKARSNDLYNQAKNPYDREKLTRPEDYSKALYTFDIGQNDLAVGF 180

Query: 181 RKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFL 240
           RKLS DQL AALPDIVNQFASA+QRIYK GGRSFWIHNTGPFGCLPVNQFY LNP PG L
Sbjct: 181 RKLSIDQLRAALPDIVNQFASAIQRIYKLGGRSFWIHNTGPFGCLPVNQFYNLNPPPGIL 240

Query: 241 DEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDP 300
           DEHGCIKAQND+S+E NNQLKA+L KL+ ELP+AAITYVD YAAKYALISNGKTQGFP+P
Sbjct: 241 DEHGCIKAQNDISMELNNQLKAKLDKLRAELPDAAITYVDVYAAKYALISNGKTQGFPEP 300

Query: 301 LKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILN 360
           LKVCCGYHVRYDHVWCGTKA INGRLV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILN
Sbjct: 301 LKVCCGYHVRYDHVWCGTKAPINGRLVFGDACENRAQYVSWDGVHYSEAANHFVANHILN 360

Query: 361 GSLSTPPIPITQACHRS 377
           G+LS+PPIPITQACHR+
Sbjct: 361 GALSSPPIPITQACHRN 377

BLAST of Moc08g14420 vs. ExPASy TrEMBL
Match: A0A1S3CMP9 (GDSL esterase/lipase At5g14450-like OS=Cucumis melo OX=3656 GN=LOC103502685 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 6.0e-187
Identity = 314/377 (83.29%), Postives = 342/377 (90.72%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVVVSSQP-CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFH 60
           MEF A  ++A  V  V V  + P C+FP IFNFGDSNSDTG ISAAFEPIR PYG+ FF+
Sbjct: 1   MEFSATALSAFLVFHVFVALASPDCNFPVIFNFGDSNSDTGAISAAFEPIRWPYGDVFFN 60

Query: 61  KPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYG 120
           KPSGRDSDGRLIIDFIAE LRLPYL+AYLNSLGANFR+GANFATGGSTVR+ NETI+EYG
Sbjct: 61  KPSGRDSDGRLIIDFIAEKLRLPYLSAYLNSLGANFRHGANFATGGSTVRKPNETIYEYG 120

Query: 121 ISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGF 180
           ISPF LD+QV QFEQFKARS DLYNQAKNP +R+KL RPED+SKALYTFDIGQNDL+VGF
Sbjct: 121 ISPFFLDMQVTQFEQFKARSNDLYNQAKNPYDREKLTRPEDYSKALYTFDIGQNDLAVGF 180

Query: 181 RKLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFL 240
           RKLS DQL AALPDIVNQFASA+QRIYK GGRSFWIHNTGPFGCLPVNQFY LNP PG L
Sbjct: 181 RKLSIDQLRAALPDIVNQFASAIQRIYKLGGRSFWIHNTGPFGCLPVNQFYNLNPPPGIL 240

Query: 241 DEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDP 300
           DEHGCIKAQND+S+E NNQLKA+L KL+ ELP+AAITYVD YAAKYALISNGKTQGFP+P
Sbjct: 241 DEHGCIKAQNDISMELNNQLKAKLDKLRAELPDAAITYVDVYAAKYALISNGKTQGFPEP 300

Query: 301 LKVCCGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILN 360
           LKVCCGYHVRYDHVWCGTKA INGRLV+G ACEN+AQ+VSWDGVHYSEAANHFVANHILN
Sbjct: 301 LKVCCGYHVRYDHVWCGTKAPINGRLVFGDACENRAQYVSWDGVHYSEAANHFVANHILN 360

Query: 361 GSLSTPPIPITQACHRS 377
           G+LS+PPIPITQACHR+
Sbjct: 361 GALSSPPIPITQACHRN 377

BLAST of Moc08g14420 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 554.7 bits (1428), Expect = 5.8e-158
Identity = 250/369 (67.75%), Postives = 303/369 (82.11%), Query Frame = 0

Query: 7   LVTAIFVLQVVVVSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGRDS 66
           L+  +F +   V     C FPAI+NFGDSNSDTGGISAAFEPIR PYG+GFFH+P+GRDS
Sbjct: 20  LLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDS 79

Query: 67  DGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFSLD 126
           DGRL IDFIAE L LPYL+AYLNSLG+NFR+GANFATGGST+RRQNETIF+YGISPFSLD
Sbjct: 80  DGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLD 139

Query: 127 IQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLSTDQ 186
           +Q+ QF+QFKARS  L+ Q K+  +R+KLPR E+F+KALYTFDIGQNDLSVGFR +S DQ
Sbjct: 140 MQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSVDQ 199

Query: 187 LHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFLDEHGCIK 246
           L A +PDIVN  ASAV+ IY+QGGR+FW+HNTGPFGCLPVN FY+  PAPG+LD+ GC+K
Sbjct: 200 LKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVK 259

Query: 247 AQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGY 306
           AQN+M++EFN +LK  ++ L+ EL +AAITYVD Y AKY ++SN K  GF +PLKVCCGY
Sbjct: 260 AQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCCGY 319

Query: 307 HVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLSTPP 366
           H +YDH+WCG K  +N   +YGG+C N    VSWDGVHY+EAAN  VA+  LNG L+ PP
Sbjct: 320 HEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPP 379

Query: 367 IPITQACHR 376
           +PIT+AC+R
Sbjct: 380 VPITRACYR 388

BLAST of Moc08g14420 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.9e-101
Identity = 184/386 (47.67%), Postives = 258/386 (66.84%), Query Frame = 0

Query: 1   MEFPAMLVTAIFVLQVVV---VSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGF 60
           ME   +LV  + +   ++     S  C+FPAIFNFGDSNSDTGG+SA+F     P G+ F
Sbjct: 1   METNLLLVKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTF 60

Query: 61  FHKPSGRDSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFE 120
           FH PSGR SDGRLIIDFIAE L LPYLNA+L+S+G+NF +GANFAT GSTVR  N TI +
Sbjct: 61  FHSPSGRFSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQ 120

Query: 121 YGISPFSLDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSV 180
            G+SP SLD+Q++QF  F  RS  + N  +    +  LP+ E FS+ALYTFDIGQNDL+ 
Sbjct: 121 SGVSPISLDVQLVQFSDFITRSQLIRN--RGGVFKKLLPKKEYFSQALYTFDIGQNDLTA 180

Query: 181 GFR-KLSTDQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILN--P 240
           G +  +++DQ+ A +PD+ +Q ++ ++++Y +GGR FWIHNT P GCLP    Y+L+  P
Sbjct: 181 GLKLNMTSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLP----YVLDRFP 240

Query: 241 APGF-LDEHGCIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKT 300
            P   +D HGC   +N+++  +N++LK R+++L+ EL EAA TYVD Y+ K  LI+  K 
Sbjct: 241 VPASQIDNHGCAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKK 300

Query: 301 QGFPDPLKVCCGYHVRYDH---VWCGTKASINGR-LVYGGACENKAQFVSWDGVHYSEAA 360
            GF  PL  CCG+  +Y+    + CG K  I G+ +V   +C + +  VSWDG+H++E  
Sbjct: 301 LGFRYPLVACCGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETT 360

Query: 361 NHFVANHILNGSLSTPPIPITQACHR 376
           N ++   I +G+ S PP+P+  AC R
Sbjct: 361 NSWIFQQINDGAFSDPPLPVKSACTR 380

BLAST of Moc08g14420 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.7e-99
Identity = 181/370 (48.92%), Postives = 237/370 (64.05%), Query Frame = 0

Query: 5   AMLVTAIFVLQVVVVSSQPCDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGR 64
           A++V  +   + +   S  C+FPA+FNFGDSNSDTG ISAA   +  P G  FF + +GR
Sbjct: 9   AIIVLLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRSAGR 68

Query: 65  DSDGRLIIDFIAEMLRLPYLNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFS 124
            SDGRLIIDFI E L LPYL  YL+S+GAN+R+GANFATGGS +R           SPF 
Sbjct: 69  HSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPTLAC-----FSPFH 128

Query: 125 LDIQVIQFEQFKARSIDLYNQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLST 184
           L  QV QF  FK R++ LYNQ              DFSKALYT DIGQNDL++GF+ ++ 
Sbjct: 129 LGTQVSQFIHFKTRTLSLYNQT------------NDFSKALYTLDIGQNDLAIGFQNMTE 188

Query: 185 DQLHAALPDIVNQFASAVQRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGF-LDEHG 244
           +QL A +P I+  F  A++ +YK+G R F IHNTGP GCLP    Y+L   P    D +G
Sbjct: 189 EQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLP----YLLKAFPAIPRDPYG 248

Query: 245 CIKAQNDMSVEFNNQLKARLVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVC 304
           C+K  N++++EFN QLK ++ +LK ELP +  TYVD Y+AKY LI+  K  GF DP   C
Sbjct: 249 CLKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPFDYC 308

Query: 305 CGYHVRYDHVWCGTKASINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLS 364
           C   +    + CG    +NG  +Y  +C+N+  F+SWDG+HY+E AN  VAN IL+GS+S
Sbjct: 309 CVGAIGRG-MGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSIS 356

Query: 365 TPPIPITQAC 374
            PP+P  +AC
Sbjct: 369 DPPLPTQKAC 356

BLAST of Moc08g14420 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 340.5 bits (872), Expect = 1.7e-93
Identity = 170/357 (47.62%), Postives = 233/357 (65.27%), Query Frame = 0

Query: 24  CDFPAIFNFGDSNSDTGGISAAFEPIRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPY 83
           C FPAIFNFGDSNSDTGG+SAAF     P+G  FF  P+GR  DGRL+IDFIAE L LPY
Sbjct: 26  CHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPY 85

Query: 84  LNAYLNSLGANFRYGANFATGGSTVRRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLY 143
           L+A+L+S+G+NF +GANFAT GS +R  N T+ + G SPFSLD+Q +QF  F  RS  + 
Sbjct: 86  LSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTV- 145

Query: 144 NQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVG-FRKLSTDQLHAALPDIVNQFASAV 203
            +++    +  LP  + FSKALYTFDIGQNDL+ G F   + +Q+   +P+I++QF +A+
Sbjct: 146 -RSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIISQFMNAI 205

Query: 204 QRIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKAR 263
           + IY QGGR FWIHNTGP GCL        N A  F D HGC+   N ++ +FN+ LK  
Sbjct: 206 KNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDF-DSHGCVSPLNHLAQQFNHALKQA 265

Query: 264 LVKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGYHVRYDH---VWCGTKA 323
           +++L++ L EAAITYVD Y+ K+ L  + +  GF   L  CCG+  +Y++   + CG K 
Sbjct: 266 VIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIGCGMKK 325

Query: 324 SINGRLVY-GGACENKAQFVSWDGVHYSEAANHFVANHILNGSLSTPPIPITQACHR 376
            + G+ VY G  C+   + V WDGVH+++AAN F+ + I  G        +++AC R
Sbjct: 326 IVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG--------LSKACKR 371

BLAST of Moc08g14420 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 6.1e-83
Identity = 155/347 (44.67%), Postives = 218/347 (62.82%), Query Frame = 0

Query: 26  FPAIFNFGDSNSDTGG-ISAAFEPIRAPYGEGFFHKPSGRDSDGRLIIDFIAEMLRLPYL 85
           +PAI NFGDSNSDTG  ISA  E +  PYG+ +F+ PSGR  DGRLI+DF+ + + LP+L
Sbjct: 30  YPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRYCDGRLIVDFLLDEMDLPFL 89

Query: 86  NAYLNSLG-ANFRYGANFATGGSTVRRQNETIFEYGISPFSLDIQVIQFEQFKARSIDLY 145
           N YL+SLG  NF+ G NFA  GST+   N T     +SPFS D+Q+ QF +FK+R+I+L 
Sbjct: 90  NPYLDSLGLPNFKKGCNFAAAGSTILPANPT----SVSPFSFDLQISQFIRFKSRAIELL 149

Query: 146 NQAKNPSERDKLPRPEDFSKALYTFDIGQNDLSVGFRKLSTDQLHAALPDIVNQFASAVQ 205
           ++     E+  LP  + +SK LY  DIGQND++  F   + DQ+ A++P I+  F + ++
Sbjct: 150 SKTGRKYEK-YLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETFEAGLK 209

Query: 206 RIYKQGGRSFWIHNTGPFGCLPVNQFYILNPAPGFLDEHGCIKAQNDMSVEFNNQLKARL 265
           R+Y++GGR+ WIHNTGP GCL  N           LDE GC+ + N  +  FN QL A  
Sbjct: 210 RLYEEGGRNIWIHNTGPLGCLAQN-IAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMS 269

Query: 266 VKLKTELPEAAITYVDAYAAKYALISNGKTQGFPDPLKVCCGY---HVRYD-HVWCGTKA 325
            K + + P+A +TYVD ++ K  LI+N    GF  PL  CCG     + YD  + CG   
Sbjct: 270 NKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGGAPLNYDSRITCGQTK 329

Query: 326 SINGRLVYGGACENKAQFVSWDGVHYSEAANHFVANHILNGSLSTPP 367
            ++G  V   AC + +++++WDG+HY+EAAN FV++ IL G  S PP
Sbjct: 330 VLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDPP 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152152.15.6e-195100.00GDSL esterase/lipase At5g14450-like, partial [Momordica charantia][more]
KAG6581060.11.1e-19084.88GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022982673.15.4e-19084.88GDSL esterase/lipase At5g14450-like [Cucurbita maxima][more]
XP_023526085.13.5e-18984.62GDSL esterase/lipase At5g14450-like [Cucurbita pepo subsp. pepo][more]
XP_022935096.16.6e-18885.22GDSL esterase/lipase At5g14450-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LY848.2e-15767.75GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2... [more]
Q9LIN24.1e-10047.67GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1... [more]
Q9LII91.3e-9849.19GDSL esterase/lipase At3g27950 OS=Arabidopsis thaliana OX=3702 GN=At3g27950 PE=2... [more]
B4FZ873.8e-9852.22GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1[more]
Q7Y1X15.2e-9546.91Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DFE72.7e-195100.00GDSL esterase/lipase At5g14450-like OS=Momordica charantia OX=3673 GN=LOC1110199... [more]
A0A6J1J5G02.6e-19084.88GDSL esterase/lipase At5g14450-like OS=Cucurbita maxima OX=3661 GN=LOC111481477 ... [more]
A0A6J1F3K83.2e-18885.22GDSL esterase/lipase At5g14450-like OS=Cucurbita moschata OX=3662 GN=LOC11144206... [more]
A0A5D3D5A16.0e-18783.29GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257... [more]
A0A1S3CMP96.0e-18783.29GDSL esterase/lipase At5g14450-like OS=Cucumis melo OX=3656 GN=LOC103502685 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G14450.15.8e-15867.75GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G26430.12.9e-10147.67GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G27950.12.7e-9948.92GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G67830.11.7e-9347.62alpha-fucosidase 1 [more]
AT1G54790.36.1e-8344.67GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 16..362
e-value: 5.0E-70
score: 238.4
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 29..357
e-value: 1.2E-46
score: 159.9
NoneNo IPR availablePANTHERPTHR22835:SF219BNAC09G43060D PROTEINcoord: 17..375
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 17..375
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 29..359
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 27..361
e-value: 3.23605E-108
score: 318.02

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g14420.1Moc08g14420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds