Moc08g14320 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g14320
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr8: 10833702 .. 10834205 (+)
RNA-Seq Expression	Moc08g14320
Synteny	Moc08g14320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTCATGGACCTGCAAAGGTTATGACTTATGGAGGATCTAGATATTTCCTTACCCTTATAGATGATTTTTCTAGAAAACTATGGGTTTATTTGCTTAAAACAAAAGATGAGGTTTTTGACAAATTTTTTGAGTGGAAGGTGTTAGTTGAAAATCAAATTGGGAGAAGAATAAAGATGTTGAGAACAGACAATGGCTTGGAGTTTTTAAGTGAAAAGTTTAAACAACTATGTAACACCTCTGGGATTGGTAGACATCTTATTGTGCCTGGTACTCCCCAACAAAATGGCTTAGTTGAACGTTTTAATAGAACGATTCTTGAAAAGGTGAGATGTATGCTTTCAAATGCAAATTTACCTAAAGTGTTTTGGGGTGAGGCTGTGAATACAGCCACCTATCTTATAAATAGGTGCCCTTCAACTGCTTTAAACTTCAAGACACCTCAAGAGGTAATGGTCAGGAAAACCTCCTTCATTAGCTCACTTGAGAACTTTTGGTTGTGA

mRNA sequence

ATGACTCATGGACCTGCAAAGGTTATGACTTATGGAGGATCTAGATATTTCCTTACCCTTATAGATGATTTTTCTAGAAAACTATGGGTTTATTTGCTTAAAACAAAAGATGAGGTTTTTGACAAATTTTTTGAGTGGAAGGTGTTAGTTGAAAATCAAATTGGGAGAAGAATAAAGATGTTGAGAACAGACAATGGCTTGGAGTTTTTAAGTGAAAAGTTTAAACAACTATGTAACACCTCTGGGATTGGTAGACATCTTATTGTGCCTGGTACTCCCCAACAAAATGGCTTAGTTGAACGTTTTAATAGAACGATTCTTGAAAAGGTGAGATGTATGCTTTCAAATGCAAATTTACCTAAAGTGTTTTGGGGTGAGGCTGTGAATACAGCCACCTATCTTATAAATAGGTGCCCTTCAACTGCTTTAAACTTCAAGACACCTCAAGAGGTAATGGTCAGGAAAACCTCCTTCATTAGCTCACTTGAGAACTTTTGGTTGTGA

Coding sequence (CDS)

ATGACTCATGGACCTGCAAAGGTTATGACTTATGGAGGATCTAGATATTTCCTTACCCTTATAGATGATTTTTCTAGAAAACTATGGGTTTATTTGCTTAAAACAAAAGATGAGGTTTTTGACAAATTTTTTGAGTGGAAGGTGTTAGTTGAAAATCAAATTGGGAGAAGAATAAAGATGTTGAGAACAGACAATGGCTTGGAGTTTTTAAGTGAAAAGTTTAAACAACTATGTAACACCTCTGGGATTGGTAGACATCTTATTGTGCCTGGTACTCCCCAACAAAATGGCTTAGTTGAACGTTTTAATAGAACGATTCTTGAAAAGGTGAGATGTATGCTTTCAAATGCAAATTTACCTAAAGTGTTTTGGGGTGAGGCTGTGAATACAGCCACCTATCTTATAAATAGGTGCCCTTCAACTGCTTTAAACTTCAAGACACCTCAAGAGGTAATGGTCAGGAAAACCTCCTTCATTAGCTCACTTGAGAACTTTTGGTTGTGA

Protein sequence

MTHGPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRTDNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVFWGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENFWL

Homology

[Close]

BLAST of Moc08g14320 vs. NCBI nr
Match: KYP63308.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan])

HSP 1 Score: 229.6 bits (584), Expect = 2.0e-56
Identity = 106/162 (65.43%), Postives = 128/162 (79.01%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP++  ++GG+RYFL+++DDFSRK+WVY LK K+EVFD+F EWKVLVE QIGR +K LRT
Sbjct: 29  GPSRTESHGGARYFLSIVDDFSRKVWVYFLKQKNEVFDRFKEWKVLVETQIGRNLKKLRT 88

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF  EKF + C  +G+ RH  V GTPQ+NGLVERFNRTILE+VRCML++A LPK F
Sbjct: 89  DNGLEFCDEKFSKFCRENGVARHKTVRGTPQKNGLVERFNRTILERVRCMLNHAGLPKSF 148

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           W EAV++A Y INRCPSTAL FKTPQEV   +    S L  F
Sbjct: 149 WAEAVSSAVYCINRCPSTALEFKTPQEVWSGRKVDYSELRVF 190

[Close]

BLAST of Moc08g14320 vs. NCBI nr
Match: KYP48029.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94, partial [Cajanus cajan])

HSP 1 Score: 224.9 bits (572), Expect = 5.0e-55
Identity = 104/162 (64.20%), Postives = 126/162 (77.78%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP++  ++GG+RYFL+++DDFSRK+WVY LK K+EVFD+F EWKVLVE Q GR +K LRT
Sbjct: 147 GPSRTESHGGARYFLSIVDDFSRKVWVYFLKQKNEVFDRFKEWKVLVETQTGRNLKKLRT 206

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF  EKF + C  +G+ RH  V GTPQ+NGL ERFNRTILE+VRCML++A LPK F
Sbjct: 207 DNGLEFCDEKFSKFCRENGVARHKTVRGTPQKNGLAERFNRTILERVRCMLNHAGLPKSF 266

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           W EAV++A Y INRCPSTAL FKTPQEV   +    S L  F
Sbjct: 267 WVEAVSSAVYCINRCPSTALEFKTPQEVWSGRKVDYSELRVF 308

[Close]

BLAST of Moc08g14320 vs. NCBI nr
Match: KAA0043826.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 222.2 bits (565), Expect = 3.2e-54
Identity = 103/162 (63.58%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP K ++ GGSRYF+++IDDFSRK+W+Y LK KDE F KF EWK  VENQ GR++K LRT
Sbjct: 377 GPTKEVSMGGSRYFISIIDDFSRKVWIYPLKQKDEAFGKFLEWKKQVENQTGRKVKYLRT 436

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF++ KF Q C + GI RH  V  TPQQNGL ERFNRTI+E+ RC+L+NA+LP  F
Sbjct: 437 DNGLEFVNNKFNQFCKSEGITRHFTVTYTPQQNGLAERFNRTIMERTRCLLTNASLPLKF 496

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           WGEA  TA YLINR PSTALN KTPQEV   K   +  L  F
Sbjct: 497 WGEAAQTACYLINRSPSTALNLKTPQEVWTGKAPSLEHLRVF 538

[Close]

BLAST of Moc08g14320 vs. NCBI nr
Match: TYK13826.1 (putative polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 222.2 bits (565), Expect = 3.2e-54
Identity = 103/162 (63.58%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP K ++ GGSRYF+++IDDFSRK+W+Y LK KDE F KF EWK  VENQ GR++K LRT
Sbjct: 317 GPTKEVSMGGSRYFISIIDDFSRKVWIYPLKQKDEAFGKFLEWKKQVENQTGRKVKYLRT 376

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF++ KF Q C + GI RH  V  TPQQNGL ERFNRTI+E+ RC+L+NA+LP  F
Sbjct: 377 DNGLEFVNNKFNQFCKSEGITRHFTVTYTPQQNGLAERFNRTIMERTRCLLTNASLPLKF 436

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           WGEA  TA YLINR PSTALN KTPQEV   K   +  L  F
Sbjct: 437 WGEAAQTACYLINRSPSTALNLKTPQEVWTGKAPSLEHLRVF 478

[Close]

BLAST of Moc08g14320 vs. NCBI nr
Match: TYK25306.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 222.2 bits (565), Expect = 3.2e-54
Identity = 103/162 (63.58%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP K ++ GGSRYF+++IDDFSRK+W+Y LK KDE F KF EWK  VENQ GR++K LRT
Sbjct: 487 GPTKEVSMGGSRYFISIIDDFSRKVWIYPLKQKDEAFGKFLEWKKQVENQTGRKVKYLRT 546

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF++ KF Q C + GI RH  V  TPQQNGL ERFNRTI+E+ RC+L+NA+LP  F
Sbjct: 547 DNGLEFVNNKFNQFCKSEGITRHFTVTYTPQQNGLAERFNRTIMERTRCLLTNASLPLKF 606

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           WGEA  TA YLINR PSTALN KTPQEV   K   +  L  F
Sbjct: 607 WGEAAQTACYLINRSPSTALNLKTPQEVWTGKAPSLEHLRVF 648

[Close]

BLAST of Moc08g14320 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.6e-46
Identity = 89/162 (54.94%), Postives = 114/162 (70.37%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP ++ + GG++YF+T IDD SRKLWVY+LKTKD+VF  F ++  LVE + GR++K LR+
Sbjct: 490 GPMEIESMGGNKYFVTFIDDASRKLWVYILKTKDQVFQVFQKFHALVERETGRKLKRLRS 549

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNG E+ S +F++ C++ GI     VPGTPQ NG+ ER NRTI+EKVR ML  A LPK F
Sbjct: 550 DNGGEYTSREFEEYCSSHGIRHEKTVPGTPQHNGVAERMNRTIVEKVRSMLRMAKLPKSF 609

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           WGEAV TA YLINR PS  L F+ P+ V   K    S L+ F
Sbjct: 610 WGEAVQTACYLINRSPSVPLAFEIPERVWTNKEVSYSHLKVF 651

[Close]

BLAST of Moc08g14320 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 135.6 bits (340), Expect = 5.2e-31
Identity = 73/164 (44.51%), Postives = 92/164 (56.10%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP   +T     YF+  +D F+     YL+K K +VF  F ++    E     ++  L  
Sbjct: 490 GPITPVTLDDKNYFVIFVDQFTHYCVTYLIKYKSDVFSMFQDFVAKSEAHFNLKVVYLYI 549

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNG E+LS + +Q C   GI  HL VP TPQ NG+ ER  RTI EK R M+S A L K F
Sbjct: 550 DNGREYLSNEMRQFCVKKGISYHLTVPHTPQLNGVSERMIRTITEKARTMVSGAKLDKSF 609

Query: 124 WGEAVNTATYLINRCPSTAL--NFKTPQEVMVRKTSFISSLENF 166
           WGEAV TATYLINR PS AL  + KTP E+   K  ++  L  F
Sbjct: 610 WGEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKPYLKHLRVF 653

[Close]

BLAST of Moc08g14320 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.8e-22
Identity = 55/141 (39.01%), Postives = 82/141 (58.16%), Query Frame = 0


Query: 8   VMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRTDNGL 67
           +++    RY++  +D F+R  W+Y LK K +V D F  +K LVEN+   RI  L +DNG 
Sbjct: 515 ILSIDNYRYYVIFVDHFTRYTWLYPLKQKSQVKDTFIIFKSLVENRFQTRIGTLYSDNGG 574

Query: 68  EFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVFWGEA 127
           EF+    +   +  GI      P TP+ NGL ER +R I+E    +LS+A++PK +W  A
Sbjct: 575 EFV--VLRDYLSQHGISHFTSPPHTPEHNGLSERKHRHIVEMGLTLLSHASVPKTYWPYA 634

Query: 128 VNTATYLINRCPSTALNFKTP 149
            + A YLINR P+  L  ++P
Sbjct: 635 FSVAVYLINRLPTPLLQLQSP 653

[Close]

BLAST of Moc08g14320 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 6.4e-21
Identity = 55/143 (38.46%), Postives = 83/143 (58.04%), Query Frame = 0


Query: 8   VMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRTDNGL 67
           ++++   RY++  +D F+R  W+Y LK K +V + F  +K L+EN+   RI    +DNG 
Sbjct: 536 ILSHDNYRYYVIFVDHFTRYTWLYPLKQKSQVKETFITFKNLLENRFQTRIGTFYSDNGG 595

Query: 68  EFLS--EKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVFWG 127
           EF++  E F Q     GI      P TP+ NGL ER +R I+E    +LS+A++PK +W 
Sbjct: 596 EFVALWEYFSQ----HGISHLTSPPHTPEHNGLSERKHRHIVETGLTLLSHASIPKTYWP 655

Query: 128 EAVNTATYLINRCPSTALNFKTP 149
            A   A YLINR P+  L  ++P
Sbjct: 656 YAFAVAVYLINRLPTPLLQLESP 674

[Close]

BLAST of Moc08g14320 vs. ExPASy Swiss-Prot
Match: Q12491 (Transposon Ty2-B Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY2B-B PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.3e-10
Identity = 35/135 (25.93%), Postives = 63/135 (46.67%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLL--KTKDEVFDKFFEWKVLVENQIGRRIKML 63
           GP   +      YF++  D+ +R  WVY L  + ++ + + F      ++NQ   R+ ++
Sbjct: 670 GPVHHLPKSAPSYFISFTDEKTRFQWVYPLHDRREESILNVFTSILAFIKNQFNARVLVI 729

Query: 64  RTDNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPK 123
           + D G E+ ++   +     GI          + +G+ ER NRT+L   R +L  + LP 
Sbjct: 730 QMDRGSEYTNKTLHKFFTNRGITACYTTTADSRAHGVAERLNRTLLNDCRTLLHCSGLPN 789

Query: 124 VFWGEAVNTATYLIN 137
             W  AV  +T + N
Sbjct: 790 HLWFSAVEFSTIIRN 804

[Close]

BLAST of Moc08g14320 vs. ExPASy TrEMBL
Match: A0A151T8B9 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_017877 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 9.9e-57
Identity = 106/162 (65.43%), Postives = 128/162 (79.01%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP++  ++GG+RYFL+++DDFSRK+WVY LK K+EVFD+F EWKVLVE QIGR +K LRT
Sbjct: 29  GPSRTESHGGARYFLSIVDDFSRKVWVYFLKQKNEVFDRFKEWKVLVETQIGRNLKKLRT 88

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF  EKF + C  +G+ RH  V GTPQ+NGLVERFNRTILE+VRCML++A LPK F
Sbjct: 89  DNGLEFCDEKFSKFCRENGVARHKTVRGTPQKNGLVERFNRTILERVRCMLNHAGLPKSF 148

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           W EAV++A Y INRCPSTAL FKTPQEV   +    S L  F
Sbjct: 149 WAEAVSSAVYCINRCPSTALEFKTPQEVWSGRKVDYSELRVF 190

[Close]

BLAST of Moc08g14320 vs. ExPASy TrEMBL
Match: A0A151RZS8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Cajanus cajan OX=3821 GN=KK1_030320 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 2.4e-55
Identity = 104/162 (64.20%), Postives = 126/162 (77.78%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP++  ++GG+RYFL+++DDFSRK+WVY LK K+EVFD+F EWKVLVE Q GR +K LRT
Sbjct: 147 GPSRTESHGGARYFLSIVDDFSRKVWVYFLKQKNEVFDRFKEWKVLVETQTGRNLKKLRT 206

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF  EKF + C  +G+ RH  V GTPQ+NGL ERFNRTILE+VRCML++A LPK F
Sbjct: 207 DNGLEFCDEKFSKFCRENGVARHKTVRGTPQKNGLAERFNRTILERVRCMLNHAGLPKSF 266

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           W EAV++A Y INRCPSTAL FKTPQEV   +    S L  F
Sbjct: 267 WVEAVSSAVYCINRCPSTALEFKTPQEVWSGRKVDYSELRVF 308

[Close]

BLAST of Moc08g14320 vs. ExPASy TrEMBL
Match: A0A5D3DNU1 (Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004440 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.6e-54
Identity = 103/162 (63.58%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP K ++ GGSRYF+++IDDFSRK+W+Y LK KDE F KF EWK  VENQ GR++K LRT
Sbjct: 487 GPTKEVSMGGSRYFISIIDDFSRKVWIYPLKQKDEAFGKFLEWKKQVENQTGRKVKYLRT 546

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF++ KF Q C + GI RH  V  TPQQNGL ERFNRTI+E+ RC+L+NA+LP  F
Sbjct: 547 DNGLEFVNNKFNQFCKSEGITRHFTVTYTPQQNGLAERFNRTIMERTRCLLTNASLPLKF 606

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           WGEA  TA YLINR PSTALN KTPQEV   K   +  L  F
Sbjct: 607 WGEAAQTACYLINRSPSTALNLKTPQEVWTGKAPSLEHLRVF 648

[Close]

BLAST of Moc08g14320 vs. ExPASy TrEMBL
Match: A0A5A7U2U7 (Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00590 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.6e-54
Identity = 103/162 (63.58%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP K ++ GGSRYF+++IDDFSRK+W+Y LK KDE F KF EWK  VENQ GR++K LRT
Sbjct: 487 GPTKEVSMGGSRYFISIIDDFSRKVWIYPLKQKDEAFGKFLEWKKQVENQTGRKVKYLRT 546

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF++ KF Q C + GI RH  V  TPQQNGL ERFNRTI+E+ RC+L+NA+LP  F
Sbjct: 547 DNGLEFVNNKFNQFCKSEGITRHFTVTYTPQQNGLAERFNRTIMERTRCLLTNASLPLKF 606

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           WGEA  TA YLINR PSTALN KTPQEV   K   +  L  F
Sbjct: 607 WGEAAQTACYLINRSPSTALNLKTPQEVWTGKAPSLEHLRVF 648

[Close]

BLAST of Moc08g14320 vs. ExPASy TrEMBL
Match: A0A5D3CTV2 (Putative polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00910 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.6e-54
Identity = 103/162 (63.58%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 4   GPAKVMTYGGSRYFLTLIDDFSRKLWVYLLKTKDEVFDKFFEWKVLVENQIGRRIKMLRT 63
           GP K ++ GGSRYF+++IDDFSRK+W+Y LK KDE F KF EWK  VENQ GR++K LRT
Sbjct: 317 GPTKEVSMGGSRYFISIIDDFSRKVWIYPLKQKDEAFGKFLEWKKQVENQTGRKVKYLRT 376

Query: 64  DNGLEFLSEKFKQLCNTSGIGRHLIVPGTPQQNGLVERFNRTILEKVRCMLSNANLPKVF 123
           DNGLEF++ KF Q C + GI RH  V  TPQQNGL ERFNRTI+E+ RC+L+NA+LP  F
Sbjct: 377 DNGLEFVNNKFNQFCKSEGITRHFTVTYTPQQNGLAERFNRTIMERTRCLLTNASLPLKF 436

Query: 124 WGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSLENF 166
           WGEA  TA YLINR PSTALN KTPQEV   K   +  L  F
Sbjct: 437 WGEAAQTACYLINRSPSTALNLKTPQEVWTGKAPSLEHLRVF 478

[Close]

BLAST of Moc08g14320 vs. TAIR 10
Match: ATMG00710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.1e-11
Identity = 32/63 (50.79%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 103 NRTILEKVRCMLSNANLPKVFWGEAVNTATYLINRCPSTALNFKTPQEVMVRKTSFISSL 162
           NRTI+EKVR ML    LPK F  +A NTA ++IN+ PSTA+NF  P EV  +     S L
Sbjct: 2   NRTIIEKVRSMLCECGLPKTFRADAANTAVHIINKYPSTAINFHVPDEVWFQSVPTYSYL 61

Query: 163 ENF 166
             F
Sbjct: 62  RRF 64

The following BLAST results are available for this feature:

BLAST of Moc08g14320 vs. NCBI nr


Match Name	E-value	Identity	Description
KYP63308.1	2.0e-56	65.43	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan]	[more]
KYP48029.1	5.0e-55	64.20	Retrovirus-related Pol polyprotein from transposon TNT 1-94, partial [Cajanus ca...	[more]
KAA0043826.1	3.2e-54	63.58	putative gag-pol polyprotein [Cucumis melo var. makuwa]	[more]
TYK13826.1	3.2e-54	63.58	putative polyprotein [Cucumis melo var. makuwa]	[more]
TYK25306.1	3.2e-54	63.58	putative gag-pol polyprotein [Cucumis melo var. makuwa]	[more]

BLAST of Moc08g14320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	2.6e-46	54.94	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	5.2e-31	44.51	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]
Q9ZT94	5.8e-22	39.01	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
Q94HW2	6.4e-21	38.46	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q12491	1.3e-10	25.93	Transposon Ty2-B Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]

BLAST of Moc08g14320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A151T8B9	9.9e-57	65.43	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=...	[more]
A0A151RZS8	2.4e-55	64.20	Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Cajanu...	[more]
A0A5D3DNU1	1.6e-54	63.58	Putative gag-pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A5A7U2U7	1.6e-54	63.58	Retrotransposon protein, putative, Ty1-copia sub-class OS=Cucumis melo var. maku...	[more]
A0A5D3CTV2	1.6e-54	63.58	Putative polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488...	[more]

BLAST of Moc08g14320 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00710.1	2.1e-11	50.79	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 6..160 e-value: 4.0E-39 score: 136.0
IPR001584	Integrase, catalytic core	PFAM	PF00665	rve	coord: 7..93 e-value: 6.4E-11 score: 42.5
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 1..156 score: 24.086052
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 4..136
None	No IPR available	PANTHER	PTHR11439:SF324	RIBONUCLEASE H-LIKE DOMAIN, GAG-PRE-INTEGRASE DOMAIN, GAG-POLYPEPTIDE OF LTR COPIA-TYPE-RELATED	coord: 4..136
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 10..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g14320.1	Moc08g14320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0007165	signal transduction
molecular_function	GO:0005092	GDP-dissociation inhibitor activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0008270	zinc ion binding