Moc08g12000 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g12000
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionIAA-amino acid hydrolase ILR1-like 4
Locationchr8: 8895473 .. 8898603 (-)
RNA-Seq ExpressionMoc08g12000
SyntenyMoc08g12000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTCAGGTGGGTATCTTGGATTTTGATCGTCCATTTGTTTTTCTCAAAATTGGTGCACTCAGATTCCTCCTCCGACTCAAATAAGCTCGCGGAAATCCCCAAGGAGTTCCTCCGATTTGCCAAGGAACCGGAGCTGTTTGATTGGATGGTGGGAGTCCGGAGGAAAATTCACGAGAACCCAGAACTCGGTTATGAGGAATTCGAGACCAGTAAGCTGATTAGAGCAGAGTTGGATAAGTTGGGCATTTCTTACAAATACCCAGTTGCGGGGACTGGAGTAATCGGATTCGTCGGTTCTGGTCAGCCCCCTTTTGTCGCCATTCGAGCAGATATGGATGCTCTTGCTATGCAGGTTTTAATCGATTGGTTGACTTATAGAACTCATTTAATCGATTGATTACTCATAGTTGATTGTTCAATGTACTGTGAATTTCACCCTCCAGGTGTTTGATCATATGCCAGAACATCAATTATCGTCTAAATCTCTGTTCTTCATTTTTTGATGCTTCGACAATGAATAAAACAGCAACTTTTTTCCAGGAATTGGTGGAGTGGGAGCATAAGAGTAAGGTTCCTGGGAAGATGCATGCTTGCGGTCACGATGCCCATGTTGCGATGGCCTTAGGTGCTGCAAAGATCCTCCAGAAACATAAAGAAGAGTTGAAAGTATGCATCTCGCGGCTTTCTTTCTTCCTTTTTTTCTTTTTTTTTTGAAGTAATGAAATTATAAATTAAGAGATAGAAGTTTCCATTCTCTAAAATTTCTTGGATCTTATCAACAATTGGAAAGAGTATTTGTGGGAAGGATATCCTGTTCACGTTTATGCTTAATTTAGGATCCAGATTTTCATGTCTTTGAGTATAGGTATTGCTCTGTGATTATTTGTGTTGAATGATAATTCGGTTCTGTACATTTTTTCTTATGAGATAGCCAGCTTACTAATTTGACTCTGGAGATCAAGATTGTAGAATTGAATCTTCCACAATTAACCTTCTGTCATTAGTTCGTAAGATCTATAAACTATTTTCTTGTTATGTAGATGTGCATCACATTTTTTGTTGGAAGAAGTTTTTAAAATTAGAAAACATGACTTGAAGTTTATGTTATAAACTGAATCATATTAGGTTTGCTAATTGTTTGGTTGCATACACTTCTTGTTTTATTACTTGAAACTTCTATGTTGGATAGATATTTTTATCGTTGCTAAATATATTAACTTACCTTACTTTAGCTGATATTGGCACCAAATTGGTGTGTTTAAGGTGCATAGCTCATGATTTGATTGACTTTGCTAAAGATAGGAGAATACGTGGGTCAATAGGAGCTCCAAGGTTCAGATGTAGTGTATTCTTGTTGGCTCAAAAGTTTTAGTGGGATCCACTTATCAATTAAAATAGAAATATACCAATAAAAGGATGCCACACGAGAAATTTGGTAGGAGCTTCTACTAACAAACGTGTTGGCAAGTATGATTCCTAGAGATATTAAACATATGCGTATTTAGATGGGTGGTAGTTGTGAAGTTGGGGGGTCACAATTAAATGTCTTCTGGAAGATCTGAATTCTGATGTTAGAAAAGATGATTGAAAATTCTTTTCCCTCTTCACCCCAGTAGGGGAGGAATAAAAGAAAACATAGATGTTCTCCACCCCCCTCCCACTTGTGAGGTGGATATATTCATAACTTTTTCACCTTTAGAAAAGTTAACTCTAGCTAATGTAGTTGGTGGTTTGCTTTGATTTTCATCCACTCTCAACAGGGAACAGTGGTACTAGTTTTTCAACCAGCTGAAGAAGGAGGTGGAGGTGCTAAGAAAATAATAGAATCTGGGGTGTTGGACAATGTCGATGCTATCTTTGGGCTGCATATCGCTCATAATATACCTCTCGGTGAAGTCGCCGGACGACCGGGACCGATGTTAGCTGGAAGTGGCTTCTTTGAAGCAGTTATCAGCGGAAAAGGAGGCCATGCCGCCATTCCTCAGCACTCCATAGACCCAATTTTGGCTGCTTCTAATGTGATTGTGAGCTTACAACATCTTGTTTCAAGGGAAGCCGATCCTCTAGATTCACAGGTAAACCATTTTTTTCCGGTTTTGTTTTAATCAATAAGGAATTATGTAGCTTGTTTATTGGGTTATTAAATATTTCATGAGAGCCTTACTGAGAAACTCCACAAAACCTATAGAAAGGAATTACAAACCACTCTAGGTACTTGTTTATTGTGTTAATACATTTATTTGTTGGGTGGTTGCTGCATTAGCCTTGTGCTCAATCTTCTGCCTCTAAATATGTTTTCAAGGTTGCAATCACAATCAATAATTGTTTGCTTTGATTTGAAAATGAGAAATTCATTTACACAGTGAAATGTGCAGGTAGTAACAGTTGCCAAGTTCCAAGGAGGTGGCGCATTCAATGTAATTCCAGATTCTGTTACAATCGGTGGCACTTTCCGAGGTTTCTTGAAGGATAGTATGATTCAACTTAAGCAACGTATTAAAGAGGTACGTACAAAAATTTTAGTTGTAAAGTGAACACTTTTCACCTAGTACTTCAGCTAGTTCGTTTCCTGAAAATATCGAGCATTCGATACTAGATTTCTGAGAATCGAATGATGTTTAGGATTACTATTAAAGTAATGTGAGTCATTGTCACTCTTACCATCTTAATATGTTGATATTTTCAACATGATAGTCTTATTTGCTCCTTCTATAGGTTATCGTTGGACAAGCTTCCGTGCATCGGTGCAATGCGACCGTTGATTTTTACGAGAAAGAGAAACCGATATTCCCTCCAACCATAAATGATAAAGGCTTGCATGGTTACTTCCAAAACGTTGCAGGAGACATGGTTGGCATTGATAAGGTGAAGGAAATGCCGCCAATTATGGGATCTGAAGATTTTTCGTTTTACCAAGAGATGATCCCAGGGTACTTCTTCTTCCTTGGAATGGAGAACGAAGCGATGGGTAGGCTCGATTGCGTGCACTCGCCCTACTTCCGTATCAACGAAGACGCACTTCCTTATGGTGCAGCTCTTCATGCATCTTTGGCTACAAGATATCTTATTGAGTTCCAGTCACAAGTTACTTCACCGAAACAAGAACGACATGACGAATTATAA

mRNA sequence

ATGGCTTCTCTCAGGTGGGTATCTTGGATTTTGATCGTCCATTTGTTTTTCTCAAAATTGGTGCACTCAGATTCCTCCTCCGACTCAAATAAGCTCGCGGAAATCCCCAAGGAGTTCCTCCGATTTGCCAAGGAACCGGAGCTGTTTGATTGGATGGTGGGAGTCCGGAGGAAAATTCACGAGAACCCAGAACTCGGTTATGAGGAATTCGAGACCAGTAAGCTGATTAGAGCAGAGTTGGATAAGTTGGGCATTTCTTACAAATACCCAGTTGCGGGGACTGGAGTAATCGGATTCGTCGGTTCTGGTCAGCCCCCTTTTGTCGCCATTCGAGCAGATATGGATGCTCTTGCTATGCAGGAATTGGTGGAGTGGGAGCATAAGAGTAAGGTTCCTGGGAAGATGCATGCTTGCGGTCACGATGCCCATGTTGCGATGGCCTTAGGTGCTGCAAAGATCCTCCAGAAACATAAAGAAGAGTTGAAAGGAACAGTGGTACTAGTTTTTCAACCAGCTGAAGAAGGAGGTGGAGGTGCTAAGAAAATAATAGAATCTGGGGTGTTGGACAATGTCGATGCTATCTTTGGGCTGCATATCGCTCATAATATACCTCTCGGTGAAGTCGCCGGACGACCGGGACCGATGTTAGCTGGAAGTGGCTTCTTTGAAGCAGTTATCAGCGGAAAAGGAGGCCATGCCGCCATTCCTCAGCACTCCATAGACCCAATTTTGGCTGCTTCTAATGTGATTGTGAGCTTACAACATCTTGTTTCAAGGGAAGCCGATCCTCTAGATTCACAGGTAGTAACAGTTGCCAAGTTCCAAGGAGGTGGCGCATTCAATGTAATTCCAGATTCTGTTACAATCGGTGGCACTTTCCGAGGTTTCTTGAAGGATAGTATGATTCAACTTAAGCAACGTATTAAAGAGGTTATCGTTGGACAAGCTTCCGTGCATCGGTGCAATGCGACCGTTGATTTTTACGAGAAAGAGAAACCGATATTCCCTCCAACCATAAATGATAAAGGCTTGCATGGTTACTTCCAAAACGTTGCAGGAGACATGGTTGGCATTGATAAGGTGAAGGAAATGCCGCCAATTATGGGATCTGAAGATTTTTCGTTTTACCAAGAGATGATCCCAGGGTACTTCTTCTTCCTTGGAATGGAGAACGAAGCGATGGGTAGGCTCGATTGCGTGCACTCGCCCTACTTCCGTATCAACGAAGACGCACTTCCTTATGGTGCAGCTCTTCATGCATCTTTGGCTACAAGATATCTTATTGAGTTCCAGTCACAAGTTACTTCACCGAAACAAGAACGACATGACGAATTATAA

Coding sequence (CDS)

ATGGCTTCTCTCAGGTGGGTATCTTGGATTTTGATCGTCCATTTGTTTTTCTCAAAATTGGTGCACTCAGATTCCTCCTCCGACTCAAATAAGCTCGCGGAAATCCCCAAGGAGTTCCTCCGATTTGCCAAGGAACCGGAGCTGTTTGATTGGATGGTGGGAGTCCGGAGGAAAATTCACGAGAACCCAGAACTCGGTTATGAGGAATTCGAGACCAGTAAGCTGATTAGAGCAGAGTTGGATAAGTTGGGCATTTCTTACAAATACCCAGTTGCGGGGACTGGAGTAATCGGATTCGTCGGTTCTGGTCAGCCCCCTTTTGTCGCCATTCGAGCAGATATGGATGCTCTTGCTATGCAGGAATTGGTGGAGTGGGAGCATAAGAGTAAGGTTCCTGGGAAGATGCATGCTTGCGGTCACGATGCCCATGTTGCGATGGCCTTAGGTGCTGCAAAGATCCTCCAGAAACATAAAGAAGAGTTGAAAGGAACAGTGGTACTAGTTTTTCAACCAGCTGAAGAAGGAGGTGGAGGTGCTAAGAAAATAATAGAATCTGGGGTGTTGGACAATGTCGATGCTATCTTTGGGCTGCATATCGCTCATAATATACCTCTCGGTGAAGTCGCCGGACGACCGGGACCGATGTTAGCTGGAAGTGGCTTCTTTGAAGCAGTTATCAGCGGAAAAGGAGGCCATGCCGCCATTCCTCAGCACTCCATAGACCCAATTTTGGCTGCTTCTAATGTGATTGTGAGCTTACAACATCTTGTTTCAAGGGAAGCCGATCCTCTAGATTCACAGGTAGTAACAGTTGCCAAGTTCCAAGGAGGTGGCGCATTCAATGTAATTCCAGATTCTGTTACAATCGGTGGCACTTTCCGAGGTTTCTTGAAGGATAGTATGATTCAACTTAAGCAACGTATTAAAGAGGTTATCGTTGGACAAGCTTCCGTGCATCGGTGCAATGCGACCGTTGATTTTTACGAGAAAGAGAAACCGATATTCCCTCCAACCATAAATGATAAAGGCTTGCATGGTTACTTCCAAAACGTTGCAGGAGACATGGTTGGCATTGATAAGGTGAAGGAAATGCCGCCAATTATGGGATCTGAAGATTTTTCGTTTTACCAAGAGATGATCCCAGGGTACTTCTTCTTCCTTGGAATGGAGAACGAAGCGATGGGTAGGCTCGATTGCGTGCACTCGCCCTACTTCCGTATCAACGAAGACGCACTTCCTTATGGTGCAGCTCTTCATGCATCTTTGGCTACAAGATATCTTATTGAGTTCCAGTCACAAGTTACTTCACCGAAACAAGAACGACATGACGAATTATAA

Protein sequence

MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIHENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAKKIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDSMIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDKVKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHASLATRYLIEFQSQVTSPKQERHDEL
Homology
BLAST of Moc08g12000 vs. NCBI nr
Match: XP_022148230.1 (IAA-amino acid hydrolase ILR1-like 4 [Momordica charantia])

HSP 1 Score: 901.4 bits (2328), Expect = 3.2e-258
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH
Sbjct: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK
Sbjct: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA
Sbjct: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYLIEFQSQVTSPKQERHDEL
Sbjct: 421 SLATRYLIEFQSQVTSPKQERHDEL 445

BLAST of Moc08g12000 vs. NCBI nr
Match: XP_023524755.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 820.8 bits (2119), Expect = 5.5e-234
Identity = 399/445 (89.66%), Postives = 422/445 (94.83%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILI HLF S  VHSDSSS+S  LAEIP +FL+FAKEPELFDWMVG+RRKIH
Sbjct: 1   MASLRWVSWILIAHLFVSTSVHSDSSSNSENLAEIPIKFLQFAKEPELFDWMVGIRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAVRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKVPGKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDN+ AIFGLHIAHNIPLGEVA RPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIEAGVLDNIQAIFGLHIAHNIPLGEVAARPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 241 DPLLAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           ++QLKQRIKEVI GQASVHRCNATVDF+E EKPIFPPT+NDK LH +FQNVAGDM+GIDK
Sbjct: 301 LVQLKQRIKEVITGQASVHRCNATVDFFENEKPIFPPTVNDKELHEHFQNVAGDMLGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           V++M P+MGSEDFSFYQ++IPGYFFFLGMENE  GRLD VHSPYFRINEDALPYGAALHA
Sbjct: 361 VEDMQPLMGSEDFSFYQDLIPGYFFFLGMENETSGRLDSVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q QVTS +++RHDEL
Sbjct: 421 SLATRYLLELQPQVTSLEEKRHDEL 445

BLAST of Moc08g12000 vs. NCBI nr
Match: KAG6606732.1 (IAA-amino acid hydrolase ILR1-like 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036450.1 IAA-amino acid hydrolase ILR1-like 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 818.9 bits (2114), Expect = 2.1e-233
Identity = 398/445 (89.44%), Postives = 421/445 (94.61%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILI HLF S  VHSDSSS+S  LAEIP +FL+FAKEPELFDWMVG+RRKIH
Sbjct: 1   MASLRWVSWILIAHLFVSTSVHSDSSSNSENLAEIPIKFLQFAKEPELFDWMVGIRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAVRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKVPGKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDN+ AIFGLHIAHNIPLGEVA RPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIEAGVLDNIQAIFGLHIAHNIPLGEVAARPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 241 DPLLAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           ++QLKQRIKEVI GQASVHRCNATVDF+E EKPIFPPT+NDK LH +FQ+VAGDM+GIDK
Sbjct: 301 LVQLKQRIKEVITGQASVHRCNATVDFFENEKPIFPPTVNDKELHEHFQSVAGDMLGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VK+M P+MGSEDFSFYQ++IPGYFFFLGMENE  GRLD VHSPYFRINEDALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQDLIPGYFFFLGMENETSGRLDSVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q Q TS +++RHDEL
Sbjct: 421 SLATRYLLELQPQATSLEEKRHDEL 445

BLAST of Moc08g12000 vs. NCBI nr
Match: XP_022948944.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucurbita moschata])

HSP 1 Score: 817.8 bits (2111), Expect = 4.7e-233
Identity = 397/445 (89.21%), Postives = 421/445 (94.61%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILI HLF    VHSDSSS+S  LAEIP +FL+FAKEPELFDWMVG+RRKIH
Sbjct: 1   MASLRWVSWILIAHLFVLTSVHSDSSSNSENLAEIPIKFLQFAKEPELFDWMVGIRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAVRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSK+PGKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKIPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDN+ AIFGLHIAHNIPLGEVA RPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIEAGVLDNIQAIFGLHIAHNIPLGEVAARPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 241 DPLLAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           ++QLKQRIKEVI GQASVHRCNATVDF+E EKPIFPPT+NDK LH +FQ+VAGDM+GIDK
Sbjct: 301 LVQLKQRIKEVITGQASVHRCNATVDFFENEKPIFPPTVNDKELHEHFQSVAGDMLGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VK+M P+MGSEDFSFYQ++IPGYFFFLGMENE  GRLD VHSPYFRINEDALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQDLIPGYFFFLGMENETWGRLDSVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q QVTS +++RHDEL
Sbjct: 421 SLATRYLLELQPQVTSLEEKRHDEL 445

BLAST of Moc08g12000 vs. NCBI nr
Match: XP_022998300.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucurbita maxima])

HSP 1 Score: 815.1 bits (2104), Expect = 3.0e-232
Identity = 396/445 (88.99%), Postives = 420/445 (94.38%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILI HLF S  VHSDSSS+S  LAEIP +FL+FAKEPELFDWMVG+RRKIH
Sbjct: 1   MASLRWVSWILIAHLFVSTFVHSDSSSNSENLAEIPIKFLQFAKEPELFDWMVGIRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAVRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKV GKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKVSGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDN+ AIFGLHIAHNIPLGEVA RPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIEAGVLDNIQAIFGLHIAHNIPLGEVAARPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 241 DPLLAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           ++QLKQRIKEVI GQASVHRCNATVDF+E EKPIFPPT+NDK LH +FQNVAGDM+GIDK
Sbjct: 301 LVQLKQRIKEVITGQASVHRCNATVDFFENEKPIFPPTVNDKELHEHFQNVAGDMLGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VK+M P+MGSEDFSFYQ++IPGYFFFLGMENE  GRLD VHSPYFRINEDALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQDLIPGYFFFLGMENETSGRLDSVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q +V+  +++RHDEL
Sbjct: 421 SLATRYLLELQPRVSWSEEKRHDEL 445

BLAST of Moc08g12000 vs. ExPASy Swiss-Prot
Match: O04373 (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=1 SV=2)

HSP 1 Score: 655.2 bits (1689), Expect = 5.2e-187
Identity = 313/445 (70.34%), Postives = 378/445 (84.94%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           M+  +WVS++LI+HL    L+    S  SN L++IP +FL  AK  + FDWMVG+RR+IH
Sbjct: 1   MSFFKWVSFVLILHLLNPTLI----SCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEE ETSKL+RAEL+K+G+SYKYPVA TGV+G+VG+G  PFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           E+VEWEHKSKVPGKMHACGHDAH  M LGAAK+L++H+EEL+GTVVLVFQPAEEGGGGAK
Sbjct: 121 EMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KI+E+GVL+NV AIFGLH+ + + LG+V+ R GPMLAGSGFF+A ISGKGGHAA+PQH+I
Sbjct: 181 KIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFR F   S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
            +QLK+RI++VI  QASV+ CNATVDF E+EKP FPPT+NDK LH +F+NV+GDM+GI+ 
Sbjct: 301 FMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIEN 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
             EM P+MGSEDFSFYQ+ IPG+F F+GM+N+A   +   HSPYF +NE+ LPYGA+LHA
Sbjct: 361 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           S+ATRYL+E ++  T  K  + DEL
Sbjct: 421 SMATRYLLELKAS-TLNKSNKKDEL 440

BLAST of Moc08g12000 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 1.8e-171
Identity = 295/411 (71.78%), Postives = 340/411 (82.73%), Query Frame = 0

Query: 36  PKEFLRFAKEPELFDWMVGVRRKIHENPELGYEEFETSKLIRAELDKLGISYKYPVAGTG 95
           P   LR AKE E   WMVG+RR+IHENPELGYEEF TS+L+R ELD LGI Y++P A TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 96  VIGFVGSGQPPFVAIRADMDALAMQELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQ 155
           V+  VG+G PPFVA+RADMDAL MQE VEWEHKSKVPGKMH CGHDAHVAM LG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 156 KHKEELKGTVVLVFQPAEEGGGGAKKIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPM 215
           +H++ELKGTVVLVFQPAEEGGGGAKK+I+ G ++N++AIFG+H+A  +P+G VA RPGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 216 LAGSGFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQ 275
           +AGSGFFEAVISGKGGHAA+P H+IDPILAASNVIVSLQ LVSREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 276 GGGAFNVIPDSVTIGGTFRGFLKDSMIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIF 335
           GGGAFNVIPDSVTIGGTFR FLK+S  QLKQRI+EVIV QASV RCNA VDF +K++P F
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 336 PPTINDKGLHGYFQNVAGDMVGIDKVKEMPPIMGSEDFSFYQEMIPG-YFFFLGMENEAM 395
           PPTIN  GLH +F  VA +MVG   V++  P+MG+EDF+FY + IP  Y++FLGM NE  
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 396 GRLDCVHSPYFRINEDALPYGAALHASLATRYLIEFQSQVTSPKQERHDEL 446
           G     HSPYF INEDALPYGAAL ASLATRYL+E Q   T  K + HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLATRYLLEHQPPTTG-KAKAHDEL 442

BLAST of Moc08g12000 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 6.9e-171
Identity = 294/411 (71.53%), Postives = 339/411 (82.48%), Query Frame = 0

Query: 36  PKEFLRFAKEPELFDWMVGVRRKIHENPELGYEEFETSKLIRAELDKLGISYKYPVAGTG 95
           P   LR AKE E   WMVG+RR+IHENPELGYEEF TS+L+R ELD LGI Y++P A TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 96  VIGFVGSGQPPFVAIRADMDALAMQELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQ 155
           V+  VG+G PPFVA+RADMDAL MQE VEWEHKSKVPGKMH CGHDAHVAM LG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 156 KHKEELKGTVVLVFQPAEEGGGGAKKIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPM 215
           +H++ELKGTVVLVFQPAEEGGGGAKK+I+ G ++N++AIFG+H+A  +P+G VA RPGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 216 LAGSGFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQ 275
           +AGSGFFEAVISGKGGHAA+P H+IDPILAASNVIVSLQ LVSREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 276 GGGAFNVIPDSVTIGGTFRGFLKDSMIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIF 335
           GGGAFNVIPDSVTIGGTFR FLK+S  QLKQRI+EVIV QASV RCNA VDF +K++P F
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 336 PPTINDKGLHGYFQNVAGDMVGIDKVKEMPPIMGSEDFSFYQEMIPG-YFFFLGMENEAM 395
           PPTIN  GLH +F  VA +MVG   V++  P+MG+EDF+FY + IP  Y++FLGM NE  
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 396 GRLDCVHSPYFRINEDALPYGAALHASLATRYLIEFQSQVTSPKQERHDEL 446
           G     HSPYF INEDALPYGAAL ASLA RYL+E Q   T  K + HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLAARYLLEHQPPTTG-KAKAHDEL 442

BLAST of Moc08g12000 vs. ExPASy Swiss-Prot
Match: Q9SWX9 (IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.0e-166
Identity = 284/432 (65.74%), Postives = 354/432 (81.94%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           M+  + VS++LI+HL  S L+    S  SN L++IPK FL  AK  + FDWMVG+RR+IH
Sbjct: 1   MSFCKLVSFVLILHLLNSCLI----SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEE ETSKL++ ELDK+G+SYK PVA TGVIG+VG+G  PFVA+RADMDAL +Q
Sbjct: 61  ENPELGYEEVETSKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           E+VEWEHKSK+PGKMHACGHDAH  M LGAAK+L++H+EEL+GTV+LVFQPAEEGG GAK
Sbjct: 121 EMVEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KI+E+GVL+NV AIFGLH+++ + LG+++ R G ++AGSG F+A ISGKGGHAA+PQ +I
Sbjct: 181 KIVEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVI+SLQHLVSREADPLDSQVVTVA F+G  AFNVIPDSVTIGGTFR  L  S
Sbjct: 241 DPVLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
             QLKQRI +VI  QASV+ CNATVDF E E P FPPT+N+K LH +++NV+ DM+GI+ 
Sbjct: 301 FEQLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIEN 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
             E  P+M SEDF+FYQ+ IPG+F F+GM+N++   +   HSP+F +NE+ LPYGA+L A
Sbjct: 361 YVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLA 420

Query: 421 SLATRYLIEFQS 433
           SLATRYL++  S
Sbjct: 421 SLATRYLLDSSS 428

BLAST of Moc08g12000 vs. ExPASy Swiss-Prot
Match: P54970 (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=1 SV=2)

HSP 1 Score: 530.4 bits (1365), Expect = 1.9e-149
Identity = 258/419 (61.58%), Postives = 322/419 (76.85%), Query Frame = 0

Query: 15  LFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIHENPELGYEEFETSK 74
           L F   V S+S   +   ++I  + L FAK PE+FDWMV +RRKIHENPELGYEE ETSK
Sbjct: 14  LLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSK 73

Query: 75  LIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQELVEWEHKSKVPGK 134
           LIR+EL+ +GI Y+YPVA TGVIG++G+G+PPFVA+RADMDAL +QE VEWEHKSK+ GK
Sbjct: 74  LIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGK 133

Query: 135 MHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAKKIIESGVLDNVDAI 194
           MHACGHD HV M LGAAKIL +H+  L+GTVVL+FQPAEEG  GAKK+ E G L NV+AI
Sbjct: 134 MHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAI 193

Query: 195 FGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQ 254
           FG+H++  IP G+ A R G  LAG+G FEAVI+GKGGHAAIPQH+IDP++AAS++++SLQ
Sbjct: 194 FGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQ 253

Query: 255 HLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDSMIQLKQRIKEVIVG 314
            LVSRE DPLDS+VVTV+K  GG AFNVIPDS+TIGGT R F      QL+QR+KEVI  
Sbjct: 254 QLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQQRVKEVITK 313

Query: 315 QASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDKVKEMPPIMGSEDFS 374
           QA+VHRCNA+V+     +   PPT+N+K L+  F+ V  D++G +   E  P+MGSEDFS
Sbjct: 314 QAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFS 373

Query: 375 FYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHASLATRYLIEFQSQ 434
           ++ E IPG+F  LGM++E  G     HSP +RINED LPYGAA+HAS+A +YL E  S+
Sbjct: 374 YFAETIPGHFSLLGMQDETNGYASS-HSPLYRINEDVLPYGAAIHASMAVQYLKEKASK 429

BLAST of Moc08g12000 vs. ExPASy TrEMBL
Match: A0A6J1D2C5 (IAA-amino acid hydrolase ILR1-like 4 OS=Momordica charantia OX=3673 GN=LOC111016941 PE=3 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 1.6e-258
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH
Sbjct: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK
Sbjct: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA
Sbjct: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYLIEFQSQVTSPKQERHDEL
Sbjct: 421 SLATRYLIEFQSQVTSPKQERHDEL 445

BLAST of Moc08g12000 vs. ExPASy TrEMBL
Match: A0A6J1GBE6 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111452444 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 397/445 (89.21%), Postives = 421/445 (94.61%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILI HLF    VHSDSSS+S  LAEIP +FL+FAKEPELFDWMVG+RRKIH
Sbjct: 1   MASLRWVSWILIAHLFVLTSVHSDSSSNSENLAEIPIKFLQFAKEPELFDWMVGIRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAVRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSK+PGKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKIPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDN+ AIFGLHIAHNIPLGEVA RPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIEAGVLDNIQAIFGLHIAHNIPLGEVAARPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 241 DPLLAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           ++QLKQRIKEVI GQASVHRCNATVDF+E EKPIFPPT+NDK LH +FQ+VAGDM+GIDK
Sbjct: 301 LVQLKQRIKEVITGQASVHRCNATVDFFENEKPIFPPTVNDKELHEHFQSVAGDMLGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VK+M P+MGSEDFSFYQ++IPGYFFFLGMENE  GRLD VHSPYFRINEDALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQDLIPGYFFFLGMENETWGRLDSVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q QVTS +++RHDEL
Sbjct: 421 SLATRYLLELQPQVTSLEEKRHDEL 445

BLAST of Moc08g12000 vs. ExPASy TrEMBL
Match: A0A6J1KC46 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111492976 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.5e-232
Identity = 396/445 (88.99%), Postives = 420/445 (94.38%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRWVSWILI HLF S  VHSDSSS+S  LAEIP +FL+FAKEPELFDWMVG+RRKIH
Sbjct: 1   MASLRWVSWILIAHLFVSTFVHSDSSSNSENLAEIPIKFLQFAKEPELFDWMVGIRRKIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAVRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKV GKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGAK
Sbjct: 121 ELVEWEHKSKVSGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDN+ AIFGLHIAHNIPLGEVA RPGPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 181 KIIEAGVLDNIQAIFGLHIAHNIPLGEVAARPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 241 DPLLAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           ++QLKQRIKEVI GQASVHRCNATVDF+E EKPIFPPT+NDK LH +FQNVAGDM+GIDK
Sbjct: 301 LVQLKQRIKEVITGQASVHRCNATVDFFENEKPIFPPTVNDKELHEHFQNVAGDMLGIDK 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VK+M P+MGSEDFSFYQ++IPGYFFFLGMENE  GRLD VHSPYFRINEDALPYGAALHA
Sbjct: 361 VKDMQPLMGSEDFSFYQDLIPGYFFFLGMENETSGRLDSVHSPYFRINEDALPYGAALHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q +V+  +++RHDEL
Sbjct: 421 SLATRYLLELQPRVSWSEEKRHDEL 445

BLAST of Moc08g12000 vs. ExPASy TrEMBL
Match: A0A5A7V5Y6 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001780 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.2e-229
Identity = 394/445 (88.54%), Postives = 412/445 (92.58%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRW SWILI HLF S  VHSDSSS SN   EIPK FL+FAKE ELFDWMVG+RRKIH
Sbjct: 5   MASLRWFSWILIAHLFVSTFVHSDSSSHSNNPQEIPKRFLQFAKESELFDWMVGIRRKIH 64

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELG+EEFETSKLIR ELDKLGISYKYPVA TGVIGFVGSGQPPFVAIRADMDAL MQ
Sbjct: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKVPGKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGA 
Sbjct: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDNV+AIFGLHI HNIPLG+VAGR GPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 185 KIIEAGVLDNVNAIFGLHIVHNIPLGKVAGRSGPMLAGSGFFEAVISGKGGHAAIPQHSI 244

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           M+QLKQRIKEVI GQA VHRCNATVDF E +KPIFPPT+NDK LH +FQNVAGDM+GIDK
Sbjct: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VKEM P+MGSEDFSFYQEMIPGYFFFLGMENE  G LD VHSPYFRINEDALPYGAALHA
Sbjct: 365 VKEMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q++V SP+++RHDEL
Sbjct: 425 SLATRYLLELQTKVNSPEEKRHDEL 449

BLAST of Moc08g12000 vs. ExPASy TrEMBL
Match: A0A1S3BHS0 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103489999 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 7.5e-229
Identity = 393/445 (88.31%), Postives = 411/445 (92.36%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           MASLRW SWILI HLF S  VHSDSSS SN   EIPK FL+FAKE ELFDWMVG+RRKIH
Sbjct: 5   MASLRWFSWILIAHLFVSTFVHSDSSSHSNNPQEIPKRFLQFAKESELFDWMVGIRRKIH 64

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELG+EEFETSKLIR ELDKLGISYKYPVA TGVIGFVGSGQPPFVAIRADMDAL MQ
Sbjct: 65  ENPELGFEEFETSKLIRTELDKLGISYKYPVASTGVIGFVGSGQPPFVAIRADMDALPMQ 124

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           ELVEWEHKSKVPGKMHACGHDAHVAM LGAAKILQKH EELKGTVVLVFQPAEEGGGGA 
Sbjct: 125 ELVEWEHKSKVPGKMHACGHDAHVAMVLGAAKILQKHSEELKGTVVLVFQPAEEGGGGAM 184

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KIIE+GVLDNV+AIFGLHI HNIPLG+VAGR GPMLAGSGFFEAVISGKGGHAAIPQHSI
Sbjct: 185 KIIEAGVLDNVNAIFGLHIVHNIPLGKVAGRSGPMLAGSGFFEAVISGKGGHAAIPQHSI 244

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFR FLKDS
Sbjct: 245 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFLKDS 304

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
           M+QLKQRIKEVI GQA VHRCNATVDF E +KPIFPPT+NDK LH +FQNVAGDM+GIDK
Sbjct: 305 MVQLKQRIKEVITGQAIVHRCNATVDFLENDKPIFPPTVNDKELHEHFQNVAGDMLGIDK 364

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
           VKEM P+MGSEDFSFYQEMIPGYFFFLGMENE  G LD VHSPYFRINEDALPYGAALHA
Sbjct: 365 VKEMQPVMGSEDFSFYQEMIPGYFFFLGMENETSGHLDSVHSPYFRINEDALPYGAALHA 424

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           SLATRYL+E Q++V SP+++RH EL
Sbjct: 425 SLATRYLLELQTKVNSPEEKRHGEL 449

BLAST of Moc08g12000 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 655.2 bits (1689), Expect = 3.7e-188
Identity = 313/445 (70.34%), Postives = 378/445 (84.94%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           M+  +WVS++LI+HL    L+    S  SN L++IP +FL  AK  + FDWMVG+RR+IH
Sbjct: 1   MSFFKWVSFVLILHLLNPTLI----SCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEE ETSKL+RAEL+K+G+SYKYPVA TGV+G+VG+G  PFVA+RADMDALAMQ
Sbjct: 61  ENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           E+VEWEHKSKVPGKMHACGHDAH  M LGAAK+L++H+EEL+GTVVLVFQPAEEGGGGAK
Sbjct: 121 EMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KI+E+GVL+NV AIFGLH+ + + LG+V+ R GPMLAGSGFF+A ISGKGGHAA+PQH+I
Sbjct: 181 KIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DPILAASNVIVSLQHLVSREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFR F   S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
            +QLK+RI++VI  QASV+ CNATVDF E+EKP FPPT+NDK LH +F+NV+GDM+GI+ 
Sbjct: 301 FMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIEN 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
             EM P+MGSEDFSFYQ+ IPG+F F+GM+N+A   +   HSPYF +NE+ LPYGA+LHA
Sbjct: 361 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHA 420

Query: 421 SLATRYLIEFQSQVTSPKQERHDEL 446
           S+ATRYL+E ++  T  K  + DEL
Sbjct: 421 SMATRYLLELKAS-TLNKSNKKDEL 440

BLAST of Moc08g12000 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 586.3 bits (1510), Expect = 2.1e-167
Identity = 284/432 (65.74%), Postives = 354/432 (81.94%), Query Frame = 0

Query: 1   MASLRWVSWILIVHLFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIH 60
           M+  + VS++LI+HL  S L+    S  SN L++IPK FL  AK  + FDWMVG+RR+IH
Sbjct: 1   MSFCKLVSFVLILHLLNSCLI----SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIH 60

Query: 61  ENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQ 120
           ENPELGYEE ETSKL++ ELDK+G+SYK PVA TGVIG+VG+G  PFVA+RADMDAL +Q
Sbjct: 61  ENPELGYEEVETSKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQ 120

Query: 121 ELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAK 180
           E+VEWEHKSK+PGKMHACGHDAH  M LGAAK+L++H+EEL+GTV+LVFQPAEEGG GAK
Sbjct: 121 EMVEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAK 180

Query: 181 KIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSI 240
           KI+E+GVL+NV AIFGLH+++ + LG+++ R G ++AGSG F+A ISGKGGHAA+PQ +I
Sbjct: 181 KIVEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAI 240

Query: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDS 300
           DP+LAASNVI+SLQHLVSREADPLDSQVVTVA F+G  AFNVIPDSVTIGGTFR  L  S
Sbjct: 241 DPVLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKS 300

Query: 301 MIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDK 360
             QLKQRI +VI  QASV+ CNATVDF E E P FPPT+N+K LH +++NV+ DM+GI+ 
Sbjct: 301 FEQLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIEN 360

Query: 361 VKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHA 420
             E  P+M SEDF+FYQ+ IPG+F F+GM+N++   +   HSP+F +NE+ LPYGA+L A
Sbjct: 361 YVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLA 420

Query: 421 SLATRYLIEFQS 433
           SLATRYL++  S
Sbjct: 421 SLATRYLLDSSS 428

BLAST of Moc08g12000 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 530.4 bits (1365), Expect = 1.4e-150
Identity = 258/419 (61.58%), Postives = 322/419 (76.85%), Query Frame = 0

Query: 15  LFFSKLVHSDSSSDSNKLAEIPKEFLRFAKEPELFDWMVGVRRKIHENPELGYEEFETSK 74
           L F   V S+S   +   ++I  + L FAK PE+FDWMV +RRKIHENPELGYEE ETSK
Sbjct: 14  LLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSK 73

Query: 75  LIRAELDKLGISYKYPVAGTGVIGFVGSGQPPFVAIRADMDALAMQELVEWEHKSKVPGK 134
           LIR+EL+ +GI Y+YPVA TGVIG++G+G+PPFVA+RADMDAL +QE VEWEHKSK+ GK
Sbjct: 74  LIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGK 133

Query: 135 MHACGHDAHVAMALGAAKILQKHKEELKGTVVLVFQPAEEGGGGAKKIIESGVLDNVDAI 194
           MHACGHD HV M LGAAKIL +H+  L+GTVVL+FQPAEEG  GAKK+ E G L NV+AI
Sbjct: 134 MHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAI 193

Query: 195 FGLHIAHNIPLGEVAGRPGPMLAGSGFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQ 254
           FG+H++  IP G+ A R G  LAG+G FEAVI+GKGGHAAIPQH+IDP++AAS++++SLQ
Sbjct: 194 FGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQ 253

Query: 255 HLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRGFLKDSMIQLKQRIKEVIVG 314
            LVSRE DPLDS+VVTV+K  GG AFNVIPDS+TIGGT R F      QL+QR+KEVI  
Sbjct: 254 QLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQQRVKEVITK 313

Query: 315 QASVHRCNATVDFYEKEKPIFPPTINDKGLHGYFQNVAGDMVGIDKVKEMPPIMGSEDFS 374
           QA+VHRCNA+V+     +   PPT+N+K L+  F+ V  D++G +   E  P+MGSEDFS
Sbjct: 314 QAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFS 373

Query: 375 FYQEMIPGYFFFLGMENEAMGRLDCVHSPYFRINEDALPYGAALHASLATRYLIEFQSQ 434
           ++ E IPG+F  LGM++E  G     HSP +RINED LPYGAA+HAS+A +YL E  S+
Sbjct: 374 YFAETIPGHFSLLGMQDETNGYASS-HSPLYRINEDVLPYGAAIHASMAVQYLKEKASK 429

BLAST of Moc08g12000 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 514.2 bits (1323), Expect = 1.0e-145
Identity = 250/414 (60.39%), Postives = 315/414 (76.09%), Query Frame = 0

Query: 32  LAEIPKEFLRFAKEPELFDWMVGVRRKIHENPELGYEEFETSKLIRAELDKLGISYKYPV 91
           ++ IP  FL  AK PE+FD MV +RRKIHENPELGYEEFETSK IR+ELD +G+ Y++PV
Sbjct: 30  VSRIPINFLELAKSPEVFDSMVRIRRKIHENPELGYEEFETSKFIRSELDLIGVKYRFPV 89

Query: 92  AGTGVIGFVGSGQPPFVAIRADMDALAMQELVEWEHKSKVPGKMHACGHDAHVAMALGAA 151
           A TG+IG++G+G+PPFVA+RADMDAL +QE VEWEHKSK PGKMHACGHD HVAM LGAA
Sbjct: 90  AITGIIGYIGTGEPPFVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDGHVAMLLGAA 149

Query: 152 KILQKHKEELKGTVVLVFQPAEEGGGGAKKIIESGVLDNVDAIFGLHIAHNIPLGEVAGR 211
           KILQ+H++ L+GTVVL+FQPAEEG  GAK + E G L NV+AIFG+H++   P G+ A  
Sbjct: 150 KILQQHRQHLQGTVVLIFQPAEEGLSGAKMMREEGALKNVEAIFGIHLSPRTPFGKAASL 209

Query: 212 PGPMLAGSGFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTV 271
            G  +AG+G FEAVI+GKGGHAAIPQH+IDP++AAS++++SLQHLVSRE DP DS+VVTV
Sbjct: 210 AGSFMAGAGAFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQHLVSRETDPSDSKVVTV 269

Query: 272 AKFQGGGAFNVIPDSVTIGGTFRGFLKDSMIQLKQRIKEVIVGQASVHRCNATVDFYEKE 331
            K  GG AFNVIPDS+TIGGT R F      QL++RIKE+I  QA+VHRCNA+V+     
Sbjct: 270 TKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQERIKEIITKQAAVHRCNASVNLAPNG 329

Query: 332 KPIFPPTINDKGLHGYFQNVAGDMVGIDKVKEMPPIMGSEDFSFYQEMIPGYFFFLGMEN 391
               PPT+N+  L+  F+ V  D++G +   E  P MGSEDFS++ E IPG+F  LGM++
Sbjct: 330 NQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLLGMQD 389

Query: 392 EAMGRLDCVHSPYFRINEDALPYGAALHASLATRYLIEFQSQVTSPKQERHDEL 446
           E  G     HSP++RINED LPYGAA+HA++A +YL +  S+        HDEL
Sbjct: 390 ETQGYASS-HSPHYRINEDVLPYGAAIHATMAVQYLKDKASK--GSVSGFHDEL 438

BLAST of Moc08g12000 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 444.1 bits (1141), Expect = 1.3e-124
Identity = 212/390 (54.36%), Postives = 281/390 (72.05%), Query Frame = 0

Query: 38  EFLRFAKEPELFDWMVGVRRKIHENPELGYEEFETSKLIRAELDKLGISYKYPVAGTGVI 97
           E LR   +P+   W+  VRR IHENPEL +EE+ETS+LIR+ELD++GI Y+YP+A TG+ 
Sbjct: 75  EILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIR 134

Query: 98  GFVGSGQPPFVAIRADMDALAMQELVEWEHKSKVPGKMHACGHDAHVAMALGAAKILQKH 157
            ++GSG PPFVA+RADMDAL +QE VEWEH SKV GKMHACGHDAHV M LGAA IL+  
Sbjct: 135 AWIGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAR 194

Query: 158 KEELKGTVVLVFQPAEEGGGGAKKIIESGVLDNVDAIFGLHIAHNIPLGEVAGRPGPMLA 217
           +  LKGTVVL+FQPAEE G GAK +IE G LD+V+AIF +H++H  P G +  R GP+LA
Sbjct: 195 EHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLA 254

Query: 218 GSGFFEAVISGKGGHAAIPQHSIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGG 277
           G G F AVI+ +    A      + +LAAS+ ++SLQ +VSREA PLDSQVV+V  F GG
Sbjct: 255 GCGIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGG 314

Query: 278 GAFNVIPDSVTIGGTFRGFLKDSMIQLKQRIKEVIVGQASVHRCNATVDFYEKEKPIFPP 337
            + +V PD+V +GGTFR F   S   LK+RI+EV++ Q  V  C ATV+F+EK+  I+PP
Sbjct: 315 HSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPP 374

Query: 338 TINDKGLHGYFQNVAGDMVGIDKVKEMPPIMGSEDFSFYQEMIPGYFFFLGMENEAMGRL 397
           T N+   + + + V  D++G       P +MG+EDF+FY E+IP  F+F+G+ NE +G +
Sbjct: 375 TTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSV 434

Query: 398 DCVHSPYFRINEDALPYGAALHASLATRYL 428
              HSP+F I+ED+LP GAA+HA++A RYL
Sbjct: 435 HIAHSPHFMIDEDSLPVGAAVHAAVAERYL 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148230.13.2e-258100.00IAA-amino acid hydrolase ILR1-like 4 [Momordica charantia][more]
XP_023524755.15.5e-23489.66IAA-amino acid hydrolase ILR1-like 4 [Cucurbita pepo subsp. pepo][more]
KAG6606732.12.1e-23389.44IAA-amino acid hydrolase ILR1-like 4, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022948944.14.7e-23389.21IAA-amino acid hydrolase ILR1-like 4 [Cucurbita moschata][more]
XP_022998300.13.0e-23288.99IAA-amino acid hydrolase ILR1-like 4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O043735.2e-18770.34IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=... [more]
Q84XG91.8e-17171.78IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q8S9S46.9e-17171.53IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9SWX93.0e-16665.74IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=... [more]
P549701.9e-14961.58IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1D2C51.6e-258100.00IAA-amino acid hydrolase ILR1-like 4 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A6J1GBE62.3e-23389.21IAA-amino acid hydrolase ILR1-like 4 OS=Cucurbita moschata OX=3662 GN=LOC1114524... [more]
A0A6J1KC461.5e-23288.99IAA-amino acid hydrolase ILR1-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111492976... [more]
A0A5A7V5Y61.2e-22988.54IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BHS07.5e-22988.31IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103489999 PE=... [more]
Match NameE-valueIdentityDescription
AT1G51760.13.7e-18870.34peptidase M20/M25/M40 family protein [more]
AT1G51780.12.1e-16765.74IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56660.11.4e-15061.58IAA-leucine resistant (ILR)-like 2 [more]
AT5G56650.11.0e-14560.39IAA-leucine resistant (ILR)-like 1 [more]
AT1G44350.11.3e-12454.36IAA-leucine resistant (ILR)-like gene 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 109..423
e-value: 3.6E-35
score: 121.9
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 218..312
e-value: 1.3E-9
score: 37.9
NoneNo IPR availableGENE3D3.30.70.360coord: 217..335
e-value: 6.1E-143
score: 478.0
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 52..412
e-value: 6.1E-143
score: 478.0
NoneNo IPR availablePANTHERPTHR11014:SF119IAA-AMINO ACID HYDROLASE ILR1-LIKE 4coord: 1..445
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 47..428
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 38..429
e-value: 8.0E-131
score: 434.4
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 52..414
e-value: 2.6E-116
score: 386.7
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 1..445
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 52..427
e-value: 0.0
score: 657.085
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 217..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g12000.1Moc08g12000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010179 IAA-Ala conjugate hydrolase activity
molecular_function GO:0016787 hydrolase activity