Moc08g10330 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g10330
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	dTMP kinase
Location	chr8: 7692823 .. 7697056 (-)
RNA-Seq Expression	Moc08g10330
Synteny	Moc08g10330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGTTTCGGTTTCATCTGTAAGTTACTACCTCCTTCCTCTTCCGGTTTTAAGTATTCTTGGGTTTTGAGAGAACGTGTAACGAAGGTTCCTGCTGTCTTTGCAAACTGACAAACTTCATGTTTAAATTTTTCGGAAACTAAAGAATCAAGGTTGTGATGGTCCTTTCGCTTTGGTGTTTTCTTCACCACGGTCGGAGTGTTGTCAGTACTTTAGCTTTCACGATTTACCTTCGAATTTCATACGAGCAAACCAGATATTGATGTAATAATTAACTGGTCTATTACGAATGGGTGAGAGAATGTTTTGTACTGTAAACATAAAGAGAAAAAGGAACGTAAAGTCTCTTGCTCATGAAATCAGGGTCAGAAGAGAAGCAACCCACTATTATAACAACGGGAAGTGTATTCAATATTCATAGTTCCACTCTAATTTCCTAACGCTGGATTAGCTCTATTTAGTGCCGTCTGCATCTGTGACATTTACGAGTTATATTTTTTGGGAGTCAATTGACTTGGGCGTAAAGCTTTTGAGCATACACTTTGACACAGTTTCAAGGTCAGGACATGATCCATGCTCGCTTCCACATTCCATAAGCCATTGTGAGATTAGTTTCTTCTTTCACCTGTAATTTGTGATGTTTATTTTTTGGGAAATTTATTTTTATTTGTTTAGGTTTGTGGTGGGCGGGTTCGGTGGACATTTGAAATTTGTTTTAAAGGTGCAGTCCCCTTGTTTTTTCTAACAAATATGCATCACACAGTTTCTGCTTCAACCACGAGAATCTATGAAATTGTCTGGAATGATTGATATGGAAGACTTCTCTGATGTTCATTTTTCCCTTTTAGGAGTTTTGGAGCAGTAACAGCTAGAAGATCCTCAAGTGCCCTACTGAAAATTCCTTCTAATAATTTAATCCGGAAGATCCAAATGGGAACTAATCATAACTCTGTGCTAAGAGGTTGCAGCAGTAATTCTAGAGGAGCCCTGGTGGTTCTAGAAGGTTTGGACCGCTGTGGTAAGACTACACAGTCCAGTAGATTAGTCCAATACTTAGAAGGACTTGGCCATTCTGCTGAACTTTTGCGGTTTCCTGATAGAACTACAAGTGTTGGACAAATGATATCTTCCTATCTTTCAAACGAATCACAGCTGGATGACCACACAATACATCTTCTCTTCAGTGCCAATCGATGGGAGAAGAGGTTTGTTAACTTTTCTTTATACCACTTTCAGTTTAGGCCCTTAGATTTATAAACTTTCAGTACCTGATGAGTGATGGGGCTTCCAATGTTGTTATTAAGTGGCAAGTTTTTCTTAAAATGAAAAATGGTGAGACCTGGTTTTAGAAGTTCCAAGCAATGGCAGTTGAGAACTTGGTGGACCGTGGACTACAACCATTTTCGTGCATGTCGTACGCTGAGGGAAAACCTGTATATTATATCAACATTTATTTTAGATGAAGAATAGTGTATATGCACCCCAAAAGTTTGTGATTTCGGTTTAATTACTGTAATGGCTGTCAGATCGTTGATGGAAACAAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCAGCTGCCAAAGGACTTGGTATCGAATGGTGCAAGGTATAAATACTAATTAGTAATTTGGCTGAATCCTTTGTTTATGCATGTTTATGTTTTTTTTTTCTTTTTTCTTTTTATTATAAATTTGGGGTTTTTAAATGCTCTGATGTTATGTTTGTCCTTTTCAGGCACCGGAGGTTGGCTTGCTAGCTCCGGACTTGGTACTGTACCTAGACATATCTCCAGAGGTTAGTTAAATTAGTATATCATTCTTTTTGGGGAAGAAATTTAAATGCATTTATTGGGATGGGCTTACCATAATTTACCTTTTTCTAACTTGTAAATGTAATGATGCTATGATGTAAATTTATTGACTATCTTATATTGAAAATAAATGATTTTGTACTAATTTTATGATACAATTTAATTCTGAAATTTTGTCATATTTTTATTTTTAATTCAAGCTAAAATTTTTTTTGCATATCACCCATACTGATTCAAGCCACTAAATCATCTTTCACAGATCTTTCTAGTCATAGCTGATATATGTTACATTTGTATTGGGTTTTTTATGGAACTCTATCAATTGTATTGGTAGCATAGAGTAAGCAATTGCTCAAGTAGAGCAGGTCTTATAGAGACTTCTTTTGAAGTTTTATTATGTAATTGATGGCAATGGACTTTTGAGAATAACAATTTATAATCAAGGGAATAATGTCCTTTGTGCAAACTATGATATCAATTTATAACCTAGGAGCTGGTTAGATTTAATACCTCTTTTTGAGTTTCAGTGTCTTAGGCTTTTTGTAATTACCACTTAGGTGTCATTCTTTTAGATTGAGGTCCATTCTTAATGGTAGTTCCCTTTTGTCGGGGTTGTTTTTTGTATGCCCCTTGTATATTCTTTCATTTTCTGTTAATGAAAGCTAGGTTTTTGTTTTGACTTTGATCTGCAACCCCTGGTAACTTCATAAATTCTCAATTTTAAAACTTCCAATTTGTATTATGATTGATTAATGATAAAGAGTTCTGAATGATCAGAAAGCTGTAGAAAGAGGAGGTTATGGATGCGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTACCAAGTTTTGCATGATTCCTCTTGGAAGGTAACTTGTTTTTCATTTGTGTCTTGGAAATATTGACACTCCAAGTAAATCAAAACAATAATATTCAATGAAGTTATGCACGAGGAATCCAATGTTTTTTCACGTTCATCTTAGGTGGAAGCCTGTTTTTCATATTAAAAAAAAAAAGATTGACTGACTGCATTTTGGACTTTCCTTGATCTCATTGCAGATAATTGATGCTTGCCAACCCATGGAAGATATTGAGAAGCAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGACGCCACAACGGGGAACCGCTTTCCGAGCTTTGGTTAAGTTGATGCTTTCTTTTGAAGCCTGAAGATCTTTTTCCCGGGTGTATTTATATTCAAAATGTTGAGATTTTGGGGGCTTGTCCTGTGTGTTTTTATATTCTTTTTACATCTATATAATTTTAGTTTGCTTTTCTTTATCACAAATTTTTAGCGTTCAAGAATTATAGGCATTATAAATCAAAGAAAAGTCTCTGTCATTACACTTCTATCAGATCTTCCCCCCCCCCCCCCCCCAATTGGGCTACACCAATATTTGAGAACACTGAAAGACTTATAATTTTTTAAATTATTAATTAGAGCAAGACTAATTCATTCTAATCTTGAAAATTTTGAGAGGCTAAGGATCTCTAGACACCAAAATATTAGTGGTATATCTAGTCCATGTATAGAAGTTTTACGTTTCATAGACTGACATCCATAAGTAATCATCAAGGATACATAAGTTCAAAGTTAAACATTACAGAAAACTTTTAAGACAGTAAAAAACTGTTATGGTCAACACAGTTGTTGGTAGCTGCTAGTTACTGCAATTCAAAAAGAAGAATTCAGCTCCTGGATGGTTATGGAATCATGGAGACTGAAATGGAGGTGTGAAGGGTATTTCAGGTGGAAGCGGCACCAAGGGCTCATTCAAACCAGTATCTGGTGCTGGTGGTGCAGACACAAAGGGAAATGGTTCCTGTGGAGTATCTGGAACTTCAGGAACCAGGGGAGGTGGAAGAAAGGTAACAGGTGGCTGTGGAATTGTGAAAGGTAGTGGCTCGGTTGGGATTGATACCAGTGGTGGAGGAAATATAGGGATGAACGGCGGTTCGTCAGGTGTGAGTTCAGGAGGCGGCAGATCAGGCACCGGCGGAGGTAGTGAAAATTCTGGCAGAGGTGGTGGGAACATTACTAAAGGTGTTGGTGTAGAAGGAATCAAAGGTGTGGGAGTGGGAGTTGATAATAGTGGTGGTGGTGAAGGAACCAGAGGTGGCGGAAACCATGTCGAAGGCGTTGGAGTAGATAATAGCGGTGGTGGTGAAGGAACCAAAGGTGGTGGAACCATTTCTAAAGGTGTAGGAGAAGATAGTAGTGGTGGTGGTGGTGAAGGAACCAAGGGCGGCGGGGTAGATGAAGGAGGTGGAGATGGAGTTTCAGGTGAAGCCAGAGGCGGTGGCGGTGTCGCGAGAAGGGGAGGTGGAGAAGGAACTAATGGGGCTGGCTGA

mRNA sequence

ATGCTGTTTCGGTTTCATCTGAGTTTTGGAGCAGTAACAGCTAGAAGATCCTCAAGTGCCCTACTGAAAATTCCTTCTAATAATTTAATCCGGAAGATCCAAATGGGAACTAATCATAACTCTGTGCTAAGAGGTTGCAGCAGTAATTCTAGAGGAGCCCTGGTGGTTCTAGAAGGTTTGGACCGCTGTGGTAAGACTACACAGTCCAGTAGATTAGTCCAATACTTAGAAGGACTTGGCCATTCTGCTGAACTTTTGCGGTTTCCTGATAGAACTACAAGTGTTGGACAAATGATATCTTCCTATCTTTCAAACGAATCACAGCTGGATGACCACACAATACATCTTCTCTTCAGTGCCAATCGATGGGAGAAGAGATCGTTGATGGAAACAAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCAGCTGCCAAAGGACTTGGTATCGAATGGTGCAAGGCACCGGAGGTTGGCTTGCTAGCTCCGGACTTGGTACTGTACCTAGACATATCTCCAGAGAAAGCTGTAGAAAGAGGAGGTTATGGATGCGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTACCAAGTTTTGCATGATTCCTCTTGGAAGATAATTGATGCTTGCCAACCCATGGAAGATATTGAGAAGCAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGACGCCACAACGGGGAACCGCTTTCCGAGCTTTGGTTAAGTGGAAGCGGCACCAAGGGCTCATTCAAACCAGTATCTGGTGCTGGTGGTGCAGACACAAAGGGAAATGGTTCCTGTGGAGTATCTGGAACTTCAGGAACCAGGGGAGGTGGAAGAAAGGTAACAGGTGGCTGTGGAATTGTGAAAGGTAGTGGCTCGGTTGGGATTGATACCAGTGGTGGAGGAAATATAGGGATGAACGGCGGTTCGTCAGGTGTGAGTTCAGGAGGCGGCAGATCAGGCACCGGCGGAGGTAGTGAAAATTCTGGCAGAGGTGGTGGGAACATTACTAAAGGTGTTGGTGTAGAAGGAATCAAAGGTGTGGGAGTGGGAGTTGATAATAGTGGTGGTGGTGAAGGAACCAGAGGTGGCGGAAACCATGTCGAAGGCGTTGGAGTAGATAATAGCGGTGGTGGTGAAGGAACCAAAGGTGGTGGAACCATTTCTAAAGGTGTAGGAGAAGATAGTAGTGGTGGTGGTGGTGAAGGAACCAAGGGCGGCGGGGTAGATGAAGGAGGTGGAGATGGAGTTTCAGGTGAAGCCAGAGGCGGTGGCGGTGTCGCGAGAAGGGGAGGTGGAGAAGGAACTAATGGGGCTGGCTGA

Coding sequence (CDS)

ATGCTGTTTCGGTTTCATCTGAGTTTTGGAGCAGTAACAGCTAGAAGATCCTCAAGTGCCCTACTGAAAATTCCTTCTAATAATTTAATCCGGAAGATCCAAATGGGAACTAATCATAACTCTGTGCTAAGAGGTTGCAGCAGTAATTCTAGAGGAGCCCTGGTGGTTCTAGAAGGTTTGGACCGCTGTGGTAAGACTACACAGTCCAGTAGATTAGTCCAATACTTAGAAGGACTTGGCCATTCTGCTGAACTTTTGCGGTTTCCTGATAGAACTACAAGTGTTGGACAAATGATATCTTCCTATCTTTCAAACGAATCACAGCTGGATGACCACACAATACATCTTCTCTTCAGTGCCAATCGATGGGAGAAGAGATCGTTGATGGAAACAAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCAGCTGCCAAAGGACTTGGTATCGAATGGTGCAAGGCACCGGAGGTTGGCTTGCTAGCTCCGGACTTGGTACTGTACCTAGACATATCTCCAGAGAAAGCTGTAGAAAGAGGAGGTTATGGATGCGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTACCAAGTTTTGCATGATTCCTCTTGGAAGATAATTGATGCTTGCCAACCCATGGAAGATATTGAGAAGCAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGACGCCACAACGGGGAACCGCTTTCCGAGCTTTGGTTAAGTGGAAGCGGCACCAAGGGCTCATTCAAACCAGTATCTGGTGCTGGTGGTGCAGACACAAAGGGAAATGGTTCCTGTGGAGTATCTGGAACTTCAGGAACCAGGGGAGGTGGAAGAAAGGTAACAGGTGGCTGTGGAATTGTGAAAGGTAGTGGCTCGGTTGGGATTGATACCAGTGGTGGAGGAAATATAGGGATGAACGGCGGTTCGTCAGGTGTGAGTTCAGGAGGCGGCAGATCAGGCACCGGCGGAGGTAGTGAAAATTCTGGCAGAGGTGGTGGGAACATTACTAAAGGTGTTGGTGTAGAAGGAATCAAAGGTGTGGGAGTGGGAGTTGATAATAGTGGTGGTGGTGAAGGAACCAGAGGTGGCGGAAACCATGTCGAAGGCGTTGGAGTAGATAATAGCGGTGGTGGTGAAGGAACCAAAGGTGGTGGAACCATTTCTAAAGGTGTAGGAGAAGATAGTAGTGGTGGTGGTGGTGAAGGAACCAAGGGCGGCGGGGTAGATGAAGGAGGTGGAGATGGAGTTTCAGGTGAAGCCAGAGGCGGTGGCGGTGTCGCGAGAAGGGGAGGTGGAGAAGGAACTAATGGGGCTGGCTGA

Protein sequence

MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLSGSGTKGSFKPVSGAGGADTKGNGSCGVSGTSGTRGGGRKVTGGCGIVKGSGSVGIDTSGGGNIGMNGGSSGVSSGGGRSGTGGGSENSGRGGGNITKGVGVEGIKGVGVGVDNSGGGEGTRGGGNHVEGVGVDNSGGGEGTKGGGTISKGVGEDSSGGGGEGTKGGGVDEGGGDGVSGEARGGGGVARRGGGEGTNGAG

Homology

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BLAST of Moc08g10330 vs. NCBI nr
Match: XP_022153422.1 (thymidylate kinase-like isoform X1 [Momordica charantia])

HSP 1 Score: 514.6 bits (1324), Expect = 8.6e-142
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60
           MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL
Sbjct: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60

Query: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120
           DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA
Sbjct: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120

Query: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180
           NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI
Sbjct: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180

Query: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240
           SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC
Sbjct: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240

Query: 241 VKRRHNGEPLSELWLS 257
           VKRRHNGEPLSELWLS
Sbjct: 241 VKRRHNGEPLSELWLS 256

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BLAST of Moc08g10330 vs. NCBI nr
Match: KAA0044460.1 (thymidylate kinase isoform X2 [Cucumis melo var. makuwa] >TYK29587.1 thymidylate kinase isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 488.8 bits (1257), Expect = 5.1e-134
Identity = 286/398 (71.86%), Postives = 309/398 (77.64%), Query Frame = 0


Query: 35  MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS 94
           MGTN NS+ RG SSNSRGALVVLEGLDRCGKTTQSSRL QYLEGLGHS EL RFPDRTTS
Sbjct: 1   MGTNQNSISRGSSSNSRGALVVLEGLDRCGKTTQSSRLTQYLEGLGHSVELWRFPDRTTS 60

Query: 95  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA 154
           VGQMISSYLSNESQLDDHT+HLLFSANRWEKRSLMETKLK GTTLI+DRYSYSGVAFS A
Sbjct: 61  VGQMISSYLSNESQLDDHTVHLLFSANRWEKRSLMETKLKGGTTLILDRYSYSGVAFSCA 120

Query: 155 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD 214
           KGL +EWCKAPEVGL APDLVLYL+ISPE A ERGGYG ERYE+++FQ+KV + YQVL  
Sbjct: 121 KGLDLEWCKAPEVGLPAPDLVLYLEISPEMAAERGGYGSERYERIDFQNKVIKSYQVLLG 180

Query: 215 SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLSGSGTKGSFKPVSGAGGAD 274
           S+W+I+DAC+PME IEK+LQE VL C                GSGT GS KP+SGAGGAD
Sbjct: 181 SNWQIVDACRPMEVIEKELQETVLDC----------------GSGTNGSLKPISGAGGAD 240

Query: 275 TKGNGSCGVSGTSGTRGGGRKVTGGCGIVKGSGSVGIDTSGGGNIGMNGGSSGVSSGGGR 334
           TKGNGSCG+SGTSGTRGGGRKV GGCG VKG                         GGGR
Sbjct: 241 TKGNGSCGISGTSGTRGGGRKVLGGCGNVKG-------------------------GGGR 300

Query: 335 SGTGGGSENSGRGGGNITKGVGVEGIKGVGVGVDNSGGGEGTRGGGNHVEGVGV-DNSGG 394
           S TGGGS+N G GG N+TK          GVGVDNSGGGE TRGGGN VEGVGV DN GG
Sbjct: 301 SSTGGGSKNCGIGGENVTK----------GVGVDNSGGGEETRGGGNQVEGVGVNDNGGG 344

Query: 395 GEGTKGGGTISKGVGEDSSGGGGEGTKGGGVDEGGGDG 432
           GEGTKGGGT+S+GVG D +GGGGEGT+G G   GGGDG
Sbjct: 361 GEGTKGGGTVSEGVGVDCTGGGGEGTRGVG---GGGDG 344

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BLAST of Moc08g10330 vs. NCBI nr
Match: KAG6606822.1 (Thymidylate kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 456.1 bits (1172), Expect = 3.6e-124
Identity = 225/250 (90.00%), Postives = 237/250 (94.80%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV ARRSSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 534 LSFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 593

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 594 TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 653

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           SLMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 654 SLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 713

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 714 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 773

Query: 247 GEPLSELWLS 257
           GEPLS+LWLS
Sbjct: 774 GEPLSQLWLS 783

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BLAST of Moc08g10330 vs. NCBI nr
Match: XP_022998308.1 (thymidylate kinase isoform X1 [Cucurbita maxima])

HSP 1 Score: 454.9 bits (1169), Expect = 8.1e-124
Identity = 224/250 (89.60%), Postives = 237/250 (94.80%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV AR+SSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARKSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           SLMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 257
           GEPLS+LWLS
Sbjct: 250 GEPLSQLWLS 259

[Close]

BLAST of Moc08g10330 vs. NCBI nr
Match: KAG7036529.1 (Thymidylate kinase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-123
Identity = 224/249 (89.96%), Postives = 236/249 (94.78%), Query Frame = 0


Query: 8   SFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTT 67
           SF AV ARRSSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKTT
Sbjct: 6   SFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKTT 65

Query: 68  QSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRS 127
           QSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKRS
Sbjct: 66  QSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKRS 125

Query: 128 LMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVE 187
           LMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA E
Sbjct: 126 LMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKASE 185

Query: 188 RGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNG 247
           RGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HNG
Sbjct: 186 RGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHNG 245

Query: 248 EPLSELWLS 257
           EPLS+LWLS
Sbjct: 246 EPLSQLWLS 254

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BLAST of Moc08g10330 vs. ExPASy Swiss-Prot
Match: Q0WW55 (Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.2e-87
Identity = 168/253 (66.40%), Postives = 200/253 (79.05%), Query Frame = 0


Query: 5   FHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSS-NSRGALVVLEGLDRC 64
           F  SF A+ + RS +  L+    + +R     +N +S +R  SS   RGAL+VLEGLDR 
Sbjct: 13  FSRSFRALASPRSLNYPLQCIKRSSVR--MESSNFSSGVRTESSVKPRGALIVLEGLDRS 72

Query: 65  GKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 124
           GK+TQ ++L+ +L GLGH  EL RFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRW
Sbjct: 73  GKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRW 132

Query: 125 EKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPE 184
           EKRSLME KLK+GTTLIVDRYSYSGVAFS+AKGL IEWCKAPE+GLLAPD VLYLDISPE
Sbjct: 133 EKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPE 192

Query: 185 KAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKR 244
           +A ERGGYG ERYE++EFQ KVA +YQ L DSSWKII+A   ME++EK++Q++VL  VK 
Sbjct: 193 RAAERGGYGDERYERVEFQKKVADFYQTLGDSSWKIINASDAMEEVEKKIQQVVLDQVKE 252

Query: 245 RHNGEPLSELWLS 257
              G+PLS LW S
Sbjct: 253 CTEGKPLSLLWSS 263

[Close]

BLAST of Moc08g10330 vs. ExPASy Swiss-Prot
Match: P23919 (Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4)

HSP 1 Score: 208.0 bits (528), Expect = 2.3e-52
Identity = 100/208 (48.08%), Postives = 149/208 (71.63%), Query Frame = 0


Query: 51  RGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLD 110
           RGAL+VLEG+DR GK+TQS +LV+ L   GH AELLRFP+R+T +G+++SSYL  +S ++
Sbjct: 5   RGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVE 64

Query: 111 DHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAK-GLGIEWCKAPEVGL 170
           DH++HLLFSANRWE+  L++ KL  G TL+VDRY++SGVAF+ AK    ++WCK P+VGL
Sbjct: 65  DHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGL 124

Query: 171 LAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQ-YYQVLHDS--SWKIIDACQPM 230
             PDLVL+L +    A +RG +G ERYE   FQ +  + ++Q++ D+  +WK++DA + +
Sbjct: 125 PKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSI 184

Query: 231 EDIEKQLQEIVLGCVKRRHNGEPLSELW 255
           E + + ++ +    + R    +PL ELW
Sbjct: 185 EAVHEDIRVLSEDAI-RTATEKPLGELW 211

[Close]

BLAST of Moc08g10330 vs. ExPASy Swiss-Prot
Match: P97930 (Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 1.5e-51
Identity = 99/211 (46.92%), Postives = 146/211 (69.19%), Query Frame = 0


Query: 48  SNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNES 107
           ++ RGAL+VLEG+DR GKTTQ  +LV  L   GH AELLRFP+R+T +G++++SYL  ++
Sbjct: 2   ASRRGALIVLEGVDRAGKTTQGLKLVTALCASGHRAELLRFPERSTEIGKLLNSYLEKKT 61

Query: 108 QLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAK-GLGIEWCKAPE 167
           +L+DH++HLLFSANRWE+  L++ KL  G TL++DRY++SGVAF+ AK    ++WCK P+
Sbjct: 62  ELEDHSVHLLFSANRWEQVPLIKAKLNQGVTLVLDRYAFSGVAFTGAKENFSLDWCKQPD 121

Query: 168 VGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDS---SWKIIDAC 227
           VGL  PDL+L+L +    A  RG +G ERYE   FQ +V   +Q L +    +WK++DA 
Sbjct: 122 VGLPKPDLILFLQLQLLDAAARGEFGLERYETGTFQKQVLLCFQQLMEEKNLNWKVVDAS 181

Query: 228 QPMEDIEKQLQEIVLGCVKRRHNGEPLSELW 255
           + +E++ K+++      + R     PL ELW
Sbjct: 182 KSIEEVHKEIRAHSEDAI-RNAAQRPLGELW 211

[Close]

BLAST of Moc08g10330 vs. ExPASy Swiss-Prot
Match: Q54GN2 (Thymidylate kinase OS=Dictyostelium discoideum OX=44689 GN=dtymk PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.8e-48
Identity = 93/188 (49.47%), Postives = 128/188 (68.09%), Query Frame = 0


Query: 51  RGALVVLEGLDRCGKTTQSSRLVQYLEGLGH-SAELLRFPDRTTSVGQMISSYLSNESQL 110
           RG  ++ EG+DR GK+TQ   L  ++  +     + LRFPDRTT +GQ+I+ YL N + +
Sbjct: 18  RGLFILFEGVDRVGKSTQVQSLTNHISNVQKLPTKSLRFPDRTTPIGQIINQYLQNATNM 77

Query: 111 DDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGL 170
           DD  +HLLFS+NRWE R  +   L +GT ++VDRYSYSGVA+SAAKG+  +WC A E GL
Sbjct: 78  DDRALHLLFSSNRWEARDSILELLNNGTNIVVDRYSYSGVAYSAAKGIDFDWCYACEKGL 137

Query: 171 LAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYY-QVLHDSSWKIIDACQPMED 230
             PDL+ YL +S E A +RG YG ERYE+LEFQ K+ Q Y + L D  WKII+A + +++
Sbjct: 138 PKPDLIFYLSMSSEDATKRGEYGGERYEKLEFQKKIKQIYEEKLVDDQWKIINANRSIDE 197

Query: 231 IEKQLQEI 237
           I  ++  I
Sbjct: 198 ISNEISSI 205

[Close]

BLAST of Moc08g10330 vs. ExPASy Swiss-Prot
Match: P36590 (Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tmp1 PE=2 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 1.4e-46
Identity = 94/197 (47.72%), Postives = 127/197 (64.47%), Query Frame = 0


Query: 47  SSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNE 106
           S  +RG L+V+EGLDR GK+TQ   LV  L      AEL +FPDRTT++G+ I  YL   
Sbjct: 2   SKQNRGRLIVIEGLDRSGKSTQCQLLVDKLISQHEKAELFKFPDRTTAIGKKIDDYLKES 61

Query: 107 SQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPE 166
            QL+D  IHLLFSANRWE    +  ++  G T I+DRY++SG+AFSAAKGL  EWCK+P+
Sbjct: 62  VQLNDQVIHLLFSANRWETIQYIYEQINKGVTCILDRYAFSGIAFSAAKGLDWEWCKSPD 121

Query: 167 VGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDS------SWKII 226
            GL  PDLV++L++ P  A  RG YG ERYE++E Q KV + +Q L          +  +
Sbjct: 122 RGLPRPDLVIFLNVDPRIAATRGQYGEERYEKIEMQEKVLKNFQRLQKEFREEGLEFITL 181

Query: 227 DACQPMEDIEKQLQEIV 238
           DA   +ED+  Q+ ++V
Sbjct: 182 DASSSLEDVHSQIVDLV 198

[Close]

BLAST of Moc08g10330 vs. ExPASy TrEMBL
Match: A0A6J1DIU8 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 4.2e-142
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60
           MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL
Sbjct: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60

Query: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120
           DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA
Sbjct: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120

Query: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180
           NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI
Sbjct: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180

Query: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240
           SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC
Sbjct: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240

Query: 241 VKRRHNGEPLSELWLS 257
           VKRRHNGEPLSELWLS
Sbjct: 241 VKRRHNGEPLSELWLS 256

[Close]

BLAST of Moc08g10330 vs. ExPASy TrEMBL
Match: A0A5D3E0G4 (dTMP kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001950 PE=3 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 2.5e-134
Identity = 286/398 (71.86%), Postives = 309/398 (77.64%), Query Frame = 0


Query: 35  MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS 94
           MGTN NS+ RG SSNSRGALVVLEGLDRCGKTTQSSRL QYLEGLGHS EL RFPDRTTS
Sbjct: 1   MGTNQNSISRGSSSNSRGALVVLEGLDRCGKTTQSSRLTQYLEGLGHSVELWRFPDRTTS 60

Query: 95  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA 154
           VGQMISSYLSNESQLDDHT+HLLFSANRWEKRSLMETKLK GTTLI+DRYSYSGVAFS A
Sbjct: 61  VGQMISSYLSNESQLDDHTVHLLFSANRWEKRSLMETKLKGGTTLILDRYSYSGVAFSCA 120

Query: 155 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD 214
           KGL +EWCKAPEVGL APDLVLYL+ISPE A ERGGYG ERYE+++FQ+KV + YQVL  
Sbjct: 121 KGLDLEWCKAPEVGLPAPDLVLYLEISPEMAAERGGYGSERYERIDFQNKVIKSYQVLLG 180

Query: 215 SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLSGSGTKGSFKPVSGAGGAD 274
           S+W+I+DAC+PME IEK+LQE VL C                GSGT GS KP+SGAGGAD
Sbjct: 181 SNWQIVDACRPMEVIEKELQETVLDC----------------GSGTNGSLKPISGAGGAD 240

Query: 275 TKGNGSCGVSGTSGTRGGGRKVTGGCGIVKGSGSVGIDTSGGGNIGMNGGSSGVSSGGGR 334
           TKGNGSCG+SGTSGTRGGGRKV GGCG VKG                         GGGR
Sbjct: 241 TKGNGSCGISGTSGTRGGGRKVLGGCGNVKG-------------------------GGGR 300

Query: 335 SGTGGGSENSGRGGGNITKGVGVEGIKGVGVGVDNSGGGEGTRGGGNHVEGVGV-DNSGG 394
           S TGGGS+N G GG N+TK          GVGVDNSGGGE TRGGGN VEGVGV DN GG
Sbjct: 301 SSTGGGSKNCGIGGENVTK----------GVGVDNSGGGEETRGGGNQVEGVGVNDNGGG 344

Query: 395 GEGTKGGGTISKGVGEDSSGGGGEGTKGGGVDEGGGDG 432
           GEGTKGGGT+S+GVG D +GGGGEGT+G G   GGGDG
Sbjct: 361 GEGTKGGGTVSEGVGVDCTGGGGEGTRGVG---GGGDG 344

[Close]

BLAST of Moc08g10330 vs. ExPASy TrEMBL
Match: A0A6J1KGD6 (dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 3.9e-124
Identity = 224/250 (89.60%), Postives = 237/250 (94.80%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV AR+SSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARKSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           SLMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 257
           GEPLS+LWLS
Sbjct: 250 GEPLSQLWLS 259

[Close]

BLAST of Moc08g10330 vs. ExPASy TrEMBL
Match: A0A6J1G9Z9 (dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 8.7e-124
Identity = 224/250 (89.60%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV ARRSSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           S METKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SSMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 257
           GEPLS+LWLS
Sbjct: 250 GEPLSQLWLS 259

[Close]

BLAST of Moc08g10330 vs. ExPASy TrEMBL
Match: A0A6J1DHF1 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.9e-123
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 35  MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS 94
           MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS
Sbjct: 1   MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS 60

Query: 95  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA 154
           VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA
Sbjct: 61  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA 120

Query: 155 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD 214
           KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD
Sbjct: 121 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD 180

Query: 215 SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS 257
           SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS
Sbjct: 181 SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS 222

[Close]

BLAST of Moc08g10330 vs. TAIR 10
Match: AT5G59440.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-88
Identity = 168/253 (66.40%), Postives = 200/253 (79.05%), Query Frame = 0


Query: 5   FHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSS-NSRGALVVLEGLDRC 64
           F  SF A+ + RS +  L+    + +R     +N +S +R  SS   RGAL+VLEGLDR 
Sbjct: 13  FSRSFRALASPRSLNYPLQCIKRSSVR--MESSNFSSGVRTESSVKPRGALIVLEGLDRS 72

Query: 65  GKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 124
           GK+TQ ++L+ +L GLGH  EL RFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRW
Sbjct: 73  GKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRW 132

Query: 125 EKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPE 184
           EKRSLME KLK+GTTLIVDRYSYSGVAFS+AKGL IEWCKAPE+GLLAPD VLYLDISPE
Sbjct: 133 EKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPE 192

Query: 185 KAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKR 244
           +A ERGGYG ERYE++EFQ KVA +YQ L DSSWKII+A   ME++EK++Q++VL  VK 
Sbjct: 193 RAAERGGYGDERYERVEFQKKVADFYQTLGDSSWKIINASDAMEEVEKKIQQVVLDQVKE 252

Query: 245 RHNGEPLSELWLS 257
              G+PLS LW S
Sbjct: 253 CTEGKPLSLLWSS 263

[Close]

BLAST of Moc08g10330 vs. TAIR 10
Match: AT5G59440.3 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-88
Identity = 168/253 (66.40%), Postives = 200/253 (79.05%), Query Frame = 0


Query: 5   FHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSS-NSRGALVVLEGLDRC 64
           F  SF A+ + RS +  L+    + +R     +N +S +R  SS   RGAL+VLEGLDR 
Sbjct: 21  FSRSFRALASPRSLNYPLQCIKRSSVR--MESSNFSSGVRTESSVKPRGALIVLEGLDRS 80

Query: 65  GKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 124
           GK+TQ ++L+ +L GLGH  EL RFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRW
Sbjct: 81  GKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRW 140

Query: 125 EKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPE 184
           EKRSLME KLK+GTTLIVDRYSYSGVAFS+AKGL IEWCKAPE+GLLAPD VLYLDISPE
Sbjct: 141 EKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPE 200

Query: 185 KAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKR 244
           +A ERGGYG ERYE++EFQ KVA +YQ L DSSWKII+A   ME++EK++Q++VL  VK 
Sbjct: 201 RAAERGGYGDERYERVEFQKKVADFYQTLGDSSWKIINASDAMEEVEKKIQQVVLDQVKE 260

Query: 245 RHNGEPLSELWLS 257
              G+PLS LW S
Sbjct: 261 CTEGKPLSLLWSS 271

[Close]

BLAST of Moc08g10330 vs. TAIR 10
Match: AT5G59440.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.7e-87
Identity = 160/221 (72.40%), Postives = 186/221 (84.16%), Query Frame = 0


Query: 37  TNHNSVLRGCSS-NSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSV 96
           +N +S +R  SS   RGAL+VLEGLDR GK+TQ ++L+ +L GLGH  EL RFPDR TSV
Sbjct: 4   SNFSSGVRTESSVKPRGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDRETSV 63

Query: 97  GQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAK 156
           GQMIS+YLSN+SQLDDHTIHLLFSANRWEKRSLME KLK+GTTLIVDRYSYSGVAFS+AK
Sbjct: 64  GQMISAYLSNKSQLDDHTIHLLFSANRWEKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAK 123

Query: 157 GLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDS 216
           GL IEWCKAPE+GLLAPD VLYLDISPE+A ERGGYG ERYE++EFQ KVA +YQ L DS
Sbjct: 124 GLDIEWCKAPEIGLLAPDSVLYLDISPERAAERGGYGDERYERVEFQKKVADFYQTLGDS 183

Query: 217 SWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS 257
           SWKII+A   ME++EK++Q++VL  VK    G+PLS LW S
Sbjct: 184 SWKIINASDAMEEVEKKIQQVVLDQVKECTEGKPLSLLWSS 224

The following BLAST results are available for this feature:

BLAST of Moc08g10330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153422.1	8.6e-142	100.00	thymidylate kinase-like isoform X1 [Momordica charantia]	[more]
KAA0044460.1	5.1e-134	71.86	thymidylate kinase isoform X2 [Cucumis melo var. makuwa] >TYK29587.1 thymidylate...	[more]
KAG6606822.1	3.6e-124	90.00	Thymidylate kinase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022998308.1	8.1e-124	89.60	thymidylate kinase isoform X1 [Cucurbita maxima]	[more]
KAG7036529.1	1.1e-123	89.96	Thymidylate kinase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Moc08g10330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WW55	2.2e-87	66.40	Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1	[more]
P23919	2.3e-52	48.08	Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4	[more]
P97930	1.5e-51	46.92	Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2	[more]
Q54GN2	9.8e-48	49.47	Thymidylate kinase OS=Dictyostelium discoideum OX=44689 GN=dtymk PE=3 SV=1	[more]
P36590	1.4e-46	47.72	Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284...	[more]

BLAST of Moc08g10330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIU8	4.2e-142	100.00	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1	[more]
A0A5D3E0G4	2.5e-134	71.86	dTMP kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001950 P...	[more]
A0A6J1KGD6	3.9e-124	89.60	dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1	[more]
A0A6J1G9Z9	8.7e-124	89.60	dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1	[more]
A0A6J1DHF1	1.9e-123	100.00	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1	[more]

BLAST of Moc08g10330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59440.1	1.5e-88	66.40	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.3	1.5e-88	66.40	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.2	1.7e-87	72.40	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR039430	Thymidylate kinase-like domain	PFAM	PF02223	Thymidylate_kin	coord: 57..232 e-value: 5.0E-45 score: 153.5
IPR018094	Thymidylate kinase	TIGRFAM	TIGR00041	TIGR00041	coord: 51..232 e-value: 2.7E-37 score: 126.4
IPR018094	Thymidylate kinase	HAMAP	MF_00165	Thymidylate_kinase	coord: 53..241 score: 16.357859
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 46..255 e-value: 3.2E-68 score: 231.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 51..247
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 313..455
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 313..348
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 267..294
None	No IPR available	PANTHER	PTHR10344:SF1	THYMIDYLATE KINASE	coord: 49..251
None	No IPR available	PANTHER	PTHR10344	THYMIDYLATE KINASE	coord: 49..251
None	No IPR available	CDD	cd01672	TMPK	coord: 54..238 e-value: 6.94711E-30 score: 113.518
IPR018095	Thymidylate kinase, conserved site	PROSITE	PS01331	THYMIDYLATE_KINASE	coord: 140..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g10330.1	Moc08g10330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006233	dTDP biosynthetic process
biological_process	GO:0046940	nucleoside monophosphate phosphorylation
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004798	thymidylate kinase activity