Moc08g10310 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g10310
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease 3-like protein 1
Location	chr8: 7685634 .. 7687946 (-)
RNA-Seq Expression	Moc08g10310
Synteny	Moc08g10310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCAGACTCCTCCGTACCCTCGACGATTAAATGTCAATCTCAAAAATCTTCCTCCAATTAATCCCCGCACTGGAAATGGAAATCATCACTTCTCGATTCCGGTAAAGTTCGGTTGATTTCCTGCTTCTGTATTCGATTCTCTTTAAAATTCGAGACGAATTTGATGTTGGTTTCAACTTTTTCAGGCCAAGTTTGAGAAATCCAGATATATTAGGCGCGTACCTCACTTTACGCCGCCGAATCACCGGCCGGAAATGAAGCCGGACACCGCTGGAGCTCTGAATACGAAGACGATTCCAGAGGCTTCGTCGGATTCTTTCGTCGCGAAGAACGAGAACAGCCGGGAAATAGCTTCCACTTGCAGTTGCGGACATCGCTCAGCCAAAGAAGGTATCGGTTCAAACCTAGTTCTTCAACGTGATTGCTGTTTGCTATTTGAGGAAGATTACTGTGATGTTTCATCACTTTCTTTGAATTTTCTTCTGTGCTTTTCTTTTCATTGAATTGCCAAAGCAGTTGATACTTCACCCACCACTTGCATTCCATTGGGAGCAATTTCGCCCCCAAGCCCTCCCACTTAGTCATTTAGCATTCGTCCAGAAAATCTGAAGTGCAATTGTAAACAAAAGAGGAAAATATGGTTTTAGTAAGCATTTGGTTTCCAACATTTCCAAAACAGAACTCTGGTTAATTTCCCTGACGTGTTTAGAAAATTATCATTACTTTCCTCTCTTTCCATTTTGTTCTCCCACTGTTCATTTTTTTTTTCCTTTGCCCTGTTTTTCTTTCGATTCTCCTAAGGATGACTTGTTTGCTAAAATAGGTTAAGTTTTACGTGAATATTGATTCCAACCCACACAAATACATTATGCTTTATAAATTATTCGATCATAACTTTTCAGTAGTATAGAAAATTTTGAGAATATTTTCCGAAACCCCTAAACATCAGTGGAGTGGGTCATGAGTTGGTCTTCATACCCCTTTATTCAAAAAGAAAAAATAAAATAAAAACTCATTAAGAGAAGGAAAACGACTTCTACAACTTGTTGTTCTAAAAATTGAGAAGTAGTTTTTGCAACTCAACTAGCAAACAGCACATAAGCTCTTGCACTTTTATGGCTTCCTTGATCTTTGTTAAAGTATTTCTATTTTTGTTTTGATACTTTTTTTTCTTGTAACTTTCTGCCTTACCTATCAAAGCTGGAGTATATTACCCTTTCAATCCTGCTGCTTATTCTTATTCCCATAAGCATTTATTACTATTCGTTTCTGAAAAGCACTGTAAATCCTCCAGAATTTTCCATTTCGTATTATCCATGTATGCTTTAATGCTATAGCTTCAAATTTCCATTTTATTAGGTACTTTAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGGTCTGCACTGCAAATTACTGGAAACCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGGCCAAGCCATGCAAAAAAGTAAGTTCAATCACCATAATCAAAATGAGTACGCACACTAGTTATAAAGCAGGGTGGCCCTAAAATTTTTTTAGGGAGTGCAAAATTTTACATTTTTACTTTAATATATTTATATATAATCATTAAATTCTGAAATTTTCGGGGGTAAATTAGAGAGAAAATTTAATCTTTACATTTTTATATAATTTTAGATGGACCAAGAGGGGACAATCATCTTCCCTCCTTCCTTCTTTACTTCGCTCCTGCTAAGTTGAAACACTTTTAGTTGAGTACATGGTGTTTGATGAAAATTGAACTTTTCAATGCTTGATATTTCTTTTCTAGAGTACTTAAACAAAAGCTGTAGTTATTATTCTGCTTTCACTCTAGTGGATTACTGGTTAAAACTCGGAAAATTGGCCTTCTAACAATTACTTTTTGAAGCTTTTATAATGTTTCTGCCTTTTATATTCTTCAAAATGGGAATAACTAGATCAGATGTCACCATGGATGTTTGTGAGAAGGTGAAGGCTTCCCAGTAGAAGATAGCTGTTCAAGTTTATTTTCAGCCTTTTAACTCTGAGAAATTTCCTCAGCATATGCCAAAAATCCTAAGCAAAGAAACGATTAGTTGATTTTACAAAGTAGGATTATAAAATAGAAGTTGTTTATCTAAGTGGGATTATAGTTTATTTTGTGCAGGTTTAGGTTCAAGGTTATTGTGGAGATGAAGGGAGCTTGTGATGCAGTTTTAGAATGCTATGGAAATTCTCAGGCAAGAAAGAAAGTAGCAGCACAGAATGCTGCAGAAGGAGCATTATGGTGTTTAAAGCATTTGGGATATTCTCATCTCTAA

mRNA sequence

ATGGAGCAGACTCCTCCGTACCCTCGACGATTAAATGTCAATCTCAAAAATCTTCCTCCAATTAATCCCCGCACTGGAAATGGAAATCATCACTTCTCGATTCCGGCCAAGTTTGAGAAATCCAGATATATTAGGCGCGTACCTCACTTTACGCCGCCGAATCACCGGCCGGAAATGAAGCCGGACACCGCTGGAGCTCTGAATACGAAGACGATTCCAGAGGCTTCGTCGGATTCTTTCGTCGCGAAGAACGAGAACAGCCGGGAAATAGCTTCCACTTGCAGTTGCGGACATCGCTCAGCCAAAGAAGGTACTTTAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGGTCTGCACTGCAAATTACTGGAAACCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGGCCAAGCCATGCAAAAAAGTTTAGGTTCAAGGTTATTGTGGAGATGAAGGGAGCTTGTGATGCAGTTTTAGAATGCTATGGAAATTCTCAGGCAAGAAAGAAAGTAGCAGCACAGAATGCTGCAGAAGGAGCATTATGGTGTTTAAAGCATTTGGGATATTCTCATCTCTAA

Coding sequence (CDS)

ATGGAGCAGACTCCTCCGTACCCTCGACGATTAAATGTCAATCTCAAAAATCTTCCTCCAATTAATCCCCGCACTGGAAATGGAAATCATCACTTCTCGATTCCGGCCAAGTTTGAGAAATCCAGATATATTAGGCGCGTACCTCACTTTACGCCGCCGAATCACCGGCCGGAAATGAAGCCGGACACCGCTGGAGCTCTGAATACGAAGACGATTCCAGAGGCTTCGTCGGATTCTTTCGTCGCGAAGAACGAGAACAGCCGGGAAATAGCTTCCACTTGCAGTTGCGGACATCGCTCAGCCAAAGAAGGTACTTTAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGGTCTGCACTGCAAATTACTGGAAACCTCCTCTGTTTGAATGCTGCGAGGAAGAAGGGCCAAGCCATGCAAAAAAGTTTAGGTTCAAGGTTATTGTGGAGATGAAGGGAGCTTGTGATGCAGTTTTAGAATGCTATGGAAATTCTCAGGCAAGAAAGAAAGTAGCAGCACAGAATGCTGCAGAAGGAGCATTATGGTGTTTAAAGCATTTGGGATATTCTCATCTCTAA

Protein sequence

MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMKPDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQNAAEGALWCLKHLGYSHL

Homology

[Close]

BLAST of Moc08g10310 vs. NCBI nr
Match: XP_022153429.1 (uncharacterized protein LOC111020939 isoform X1 [Momordica charantia])

HSP 1 Score: 402.9 bits (1034), Expect = 1.5e-108
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60
           MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK
Sbjct: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60

Query: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120
           PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT
Sbjct: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120

Query: 121 ANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQNAAEGA 180
           ANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQNAAEGA
Sbjct: 121 ANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQNAAEGA 180

Query: 181 LWCLKHLGYSHL 193
           LWCLKHLGYSHL
Sbjct: 181 LWCLKHLGYSHL 192

[Close]

BLAST of Moc08g10310 vs. NCBI nr
Match: XP_022153430.1 (uncharacterized protein LOC111020939 isoform X2 [Momordica charantia])

HSP 1 Score: 297.4 bits (760), Expect = 9.1e-77
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60
           MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK
Sbjct: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60

Query: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120
           PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT
Sbjct: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120

Query: 121 ANYWKPPLFECCEEEGPSHAK 142
           ANYWKPPLFECCEEEGPSHAK
Sbjct: 121 ANYWKPPLFECCEEEGPSHAK 141

[Close]

BLAST of Moc08g10310 vs. NCBI nr
Match: XP_038878793.1 (ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 268.5 bits (685), Expect = 4.5e-68
Identity = 141/199 (70.85%), Postives = 155/199 (77.89%), Query Frame = 0


Query: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPE-- 60
           ME T   PRRLN NLKNLPPIN       +H SI AKFEKSRYIRRVP F P NHRP   
Sbjct: 1   MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNEY 60

Query: 61  ----MKPDTAG---ALNTKTIPEASSDSFVAKNENSREIASTCS-CGHRSAKEGTLEKRA 120
               MKP  +G   ALN+KTIPEA SDSFV K +NS+EIAS  S CGH S KEGT EKRA
Sbjct: 61  AGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEKRA 120

Query: 121 AKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKK 180
           AKSLLFE+CTAN+W+PPLFECCEEEGPSHAKK+RFKV VEMKGA + VLECYGN Q RKK
Sbjct: 121 AKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTRKK 180

Query: 181 VAAQNAAEGALWCLKHLGY 190
           VAA++AAEGA+W LK+LGY
Sbjct: 181 VAAEHAAEGAIWYLKNLGY 199

[Close]

BLAST of Moc08g10310 vs. NCBI nr
Match: XP_038878769.1 (ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 268.5 bits (685), Expect = 4.5e-68
Identity = 141/199 (70.85%), Postives = 155/199 (77.89%), Query Frame = 0


Query: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPE-- 60
           ME T   PRRLN NLKNLPPIN       +H SI AKFEKSRYIRRVP F P NHRP   
Sbjct: 18  MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNEY 77

Query: 61  ----MKPDTAG---ALNTKTIPEASSDSFVAKNENSREIASTCS-CGHRSAKEGTLEKRA 120
               MKP  +G   ALN+KTIPEA SDSFV K +NS+EIAS  S CGH S KEGT EKRA
Sbjct: 78  AGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEKRA 137

Query: 121 AKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKK 180
           AKSLLFE+CTAN+W+PPLFECCEEEGPSHAKK+RFKV VEMKGA + VLECYGN Q RKK
Sbjct: 138 AKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTRKK 197

Query: 181 VAAQNAAEGALWCLKHLGY 190
           VAA++AAEGA+W LK+LGY
Sbjct: 198 VAAEHAAEGAIWYLKNLGY 216

[Close]

BLAST of Moc08g10310 vs. NCBI nr
Match: XP_038878786.1 (ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 258.8 bits (660), Expect = 3.6e-65
Identity = 136/192 (70.83%), Postives = 149/192 (77.60%), Query Frame = 0


Query: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPE-- 60
           ME T   PRRLN NLKNLPPIN       +H SI AKFEKSRYIRRVP F P NHRP   
Sbjct: 18  MENTHSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNEY 77

Query: 61  ----MKPDTAG---ALNTKTIPEASSDSFVAKNENSREIASTCS-CGHRSAKEGTLEKRA 120
               MKP  +G   ALN+KTIPEA SDSFV K +NS+EIAS  S CGH S KEGT EKRA
Sbjct: 78  AGLPMKPACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEKRA 137

Query: 121 AKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKK 180
           AKSLLFE+CTAN+W+PPLFECCEEEGPSHAKK+RFKV VEMKGA + VLECYGN Q RKK
Sbjct: 138 AKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTRKK 197

Query: 181 VAAQNAAEGALW 183
           VAA++AAEGA+W
Sbjct: 198 VAAEHAAEGAIW 209

[Close]

BLAST of Moc08g10310 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.9e-25
Identity = 48/92 (52.17%), Postives = 69/92 (75.00%), Query Frame = 0


Query: 98   HRSAKEGTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDA 157
            ++    G +  + A+S LFE+C ANYWKPP F+ C+EEGPSH +KF +KV+VE+KGA   
Sbjct: 1557 NKKVYHGDMVHKTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIKGASAT 1616

Query: 158  VLECYGNSQARKKVAAQNAAEGALWCLKHLGY 190
            +LEC+ + + +KK A ++AA+GALWCLK LG+
Sbjct: 1617 LLECHSDGKLQKKAAQEHAAQGALWCLKQLGH 1648

[Close]

BLAST of Moc08g10310 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 4.9e-25
Identity = 51/88 (57.95%), Postives = 64/88 (72.73%), Query Frame = 0


Query: 102  KEGTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLEC 161
            K G    + AKSLL E C AN WKPP FECCEEEGP H K F +KVI+E++ A +  LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 162  YGNSQARKKVAAQNAAEGALWCLKHLGY 190
            YG ++A KK AA++AA+ A+WCLKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of Moc08g10310 vs. ExPASy Swiss-Prot
Match: Q9M8N2 (Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-17
Identity = 54/192 (28.12%), Postives = 96/192 (50.00%), Query Frame = 0


Query: 9   RRLNVNLKNLPPINPRT------------GNGNHHFSIPAKFEKSRYIRRVPHFTPPNHR 68
           R + ++LK++PP++P +              G   F     F+       +  F+  N +
Sbjct: 15  RSIIISLKDIPPLDPSSIPSMKPMAQDHHNVGMQRFQEKTDFKFEEEDNAISSFS--NIQ 74

Query: 69  PEMKPDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLF 128
            +     + +L  K  P+   +        S++             E    + +AKS+L 
Sbjct: 75  IDPNSTRSISLEKKLAPKPDEEHTTTTKPISKD------------DESKTRRGSAKSVLH 134

Query: 129 EVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGAC-DAVLECYGNSQARKKVAAQN 188
           E+C +  W+PP++ECC  +GP H + F +KV+VE++ +    VLEC+G+ + +KK AA++
Sbjct: 135 EMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGDPRRKKKAAAEH 192

[Close]

BLAST of Moc08g10310 vs. ExPASy TrEMBL
Match: A0A6J1DJ12 (uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020939 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 7.5e-109
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60
           MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK
Sbjct: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60

Query: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120
           PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT
Sbjct: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120

Query: 121 ANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQNAAEGA 180
           ANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQNAAEGA
Sbjct: 121 ANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQNAAEGA 180

Query: 181 LWCLKHLGYSHL 193
           LWCLKHLGYSHL
Sbjct: 181 LWCLKHLGYSHL 192

[Close]

BLAST of Moc08g10310 vs. ExPASy TrEMBL
Match: A0A6J1DGU1 (uncharacterized protein LOC111020939 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020939 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 4.4e-77
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60
           MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK
Sbjct: 1   MEQTPPYPRRLNVNLKNLPPINPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 60

Query: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120
           PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT
Sbjct: 61  PDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLFEVCT 120

Query: 121 ANYWKPPLFECCEEEGPSHAK 142
           ANYWKPPLFECCEEEGPSHAK
Sbjct: 121 ANYWKPPLFECCEEEGPSHAK 141

[Close]

BLAST of Moc08g10310 vs. ExPASy TrEMBL
Match: A0A0A0KYI7 (DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 3.0e-65
Identity = 137/206 (66.50%), Postives = 158/206 (76.70%), Query Frame = 0


Query: 1   MEQTPPY-PRRLNVNLKNLPPI-NPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPE 60
           ME  P   P RLN+NLKNLPPI NPR  +G+H  SIPAKFEKSRYIRRVP F P +HRPE
Sbjct: 19  MENNPSCPPPRLNINLKNLPPINNPRNVSGSHS-SIPAKFEKSRYIRRVPQFMPSDHRPE 78

Query: 61  MK-------------PDTAGALNTKTIPEA-SSDSFVAKNENSREIASTCS-CGHRSAKE 120
           ++              D+A ALNTKT P A S+DSFV K +  +++AS CS C H S KE
Sbjct: 79  IRSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTCKDLASRCSCCRHDSTKE 138

Query: 121 GTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYG 180
           GT EKRAAKSLLFE+CTAN+W+PPLFECCEEEGPSHAKK+RFKV +EMKG C+AV+ECYG
Sbjct: 139 GTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYG 198

Query: 181 NSQARKKVAAQNAAEGALWCLKHLGY 190
           N Q RKKVAA++AAEGALW L HLGY
Sbjct: 199 NLQTRKKVAAEHAAEGALWYLNHLGY 223

[Close]

BLAST of Moc08g10310 vs. ExPASy TrEMBL
Match: A0A5D3E0Z9 (Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001930 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.5e-64
Identity = 133/205 (64.88%), Postives = 157/205 (76.59%), Query Frame = 0


Query: 2   EQTPPYPRRLNVNLKNLPPI-NPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 61
           +  P  P RLN+NLKNLPPI NPR   G H  SIPAKFEKSRYIRRVP F P +HRPE++
Sbjct: 21  KNNPSCPPRLNINLKNLPPINNPRNVTGTHS-SIPAKFEKSRYIRRVPQFIPFDHRPEIR 80

Query: 62  --------------PDTAGALNTKTIPEA-SSDSFVAKNENSREIASTCS-CGHRSAKEG 121
                          D+A A+NTKTIP A S+D FV K +N +++AS CS C H S KEG
Sbjct: 81  SNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKEG 140

Query: 122 TLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGN 181
           T EKRAAKSLLFE+CTAN+W+PPLFECCEEEGPSHA+K+RFKV +EMKG C+AV+ECYGN
Sbjct: 141 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMKGDCEAVVECYGN 200

Query: 182 SQARKKVAAQNAAEGALWCLKHLGY 190
            Q RKK+AA++AAEGALW LK+LGY
Sbjct: 201 LQTRKKMAAEHAAEGALWYLKNLGY 224

[Close]

BLAST of Moc08g10310 vs. ExPASy TrEMBL
Match: A0A1S3BXK0 (uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.5e-64
Identity = 133/205 (64.88%), Postives = 157/205 (76.59%), Query Frame = 0


Query: 2   EQTPPYPRRLNVNLKNLPPI-NPRTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPEMK 61
           +  P  P RLN+NLKNLPPI NPR   G H  SIPAKFEKSRYIRRVP F P +HRPE++
Sbjct: 21  KNNPSCPPRLNINLKNLPPINNPRNVTGTHS-SIPAKFEKSRYIRRVPQFIPFDHRPEIR 80

Query: 62  --------------PDTAGALNTKTIPEA-SSDSFVAKNENSREIASTCS-CGHRSAKEG 121
                          D+A A+NTKTIP A S+D FV K +N +++AS CS C H S KEG
Sbjct: 81  SNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKEG 140

Query: 122 TLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGN 181
           T EKRAAKSLLFE+CTAN+W+PPLFECCEEEGPSHA+K+RFKV +EMKG C+AV+ECYGN
Sbjct: 141 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMKGDCEAVVECYGN 200

Query: 182 SQARKKVAAQNAAEGALWCLKHLGY 190
            Q RKK+AA++AAEGALW LK+LGY
Sbjct: 201 LQTRKKMAAEHAAEGALWYLKNLGY 224

[Close]

BLAST of Moc08g10310 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 115.9 bits (289), Expect = 3.5e-26
Identity = 51/88 (57.95%), Postives = 64/88 (72.73%), Query Frame = 0


Query: 102  KEGTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLEC 161
            K G    + AKSLL E C AN WKPP FECCEEEGP H K F +KVI+E++ A +  LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 162  YGNSQARKKVAAQNAAEGALWCLKHLGY 190
            YG ++A KK AA++AA+ A+WCLKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of Moc08g10310 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 115.9 bits (289), Expect = 3.5e-26
Identity = 51/88 (57.95%), Postives = 64/88 (72.73%), Query Frame = 0


Query: 102  KEGTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLEC 161
            K G    + AKSLL E C AN WKPP FECCEEEGP H K F +KVI+E++ A +  LEC
Sbjct: 1599 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1658

Query: 162  YGNSQARKKVAAQNAAEGALWCLKHLGY 190
            YG ++A KK AA++AA+ A+WCLKH G+
Sbjct: 1659 YGEARATKKGAAEHAAQAAIWCLKHSGF 1686

[Close]

BLAST of Moc08g10310 vs. TAIR 10
Match: AT1G80650.1 (RNAse THREE-like protein 1 )

HSP 1 Score: 91.3 bits (225), Expect = 9.3e-19
Identity = 54/192 (28.12%), Postives = 96/192 (50.00%), Query Frame = 0


Query: 9   RRLNVNLKNLPPINPRT------------GNGNHHFSIPAKFEKSRYIRRVPHFTPPNHR 68
           R + ++LK++PP++P +              G   F     F+       +  F+  N +
Sbjct: 15  RSIIISLKDIPPLDPSSIPSMKPMAQDHHNVGMQRFQEKTDFKFEEEDNAISSFS--NIQ 74

Query: 69  PEMKPDTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSLLF 128
            +     + +L  K  P+   +        S++             E    + +AKS+L 
Sbjct: 75  IDPNSTRSISLEKKLAPKPDEEHTTTTKPISKD------------DESKTRRGSAKSVLH 134

Query: 129 EVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGAC-DAVLECYGNSQARKKVAAQN 188
           E+C +  W+PP++ECC  +GP H + F +KV+VE++ +    VLEC+G+ + +KK AA++
Sbjct: 135 EMCASKRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGDPRRKKKAAAEH 192

[Close]

BLAST of Moc08g10310 vs. TAIR 10
Match: AT4G00420.2 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-17
Identity = 44/89 (49.44%), Postives = 60/89 (67.42%), Query Frame = 0


Query: 103 EGTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMK-GACDAVLEC 162
           E   ++ +AKS L+ +C+  +WK PL+E    EGP H K F  KV VEMK  +   VLEC
Sbjct: 77  EEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVLEC 136

Query: 163 YGNSQARKKVAAQNAAEGALWCLKHLGYS 191
           +GN Q +KK+AA+ AAE ALW LK++GY+
Sbjct: 137 FGNPQYKKKIAAEQAAEAALWYLKNVGYT 164

[Close]

BLAST of Moc08g10310 vs. TAIR 10
Match: AT4G00420.3 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-17
Identity = 44/89 (49.44%), Postives = 60/89 (67.42%), Query Frame = 0


Query: 103 EGTLEKRAAKSLLFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMK-GACDAVLEC 162
           E   ++ +AKS L+ +C+  +WK PL+E    EGP H K F  KV VEMK  +   VLEC
Sbjct: 55  EEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVLEC 114

Query: 163 YGNSQARKKVAAQNAAEGALWCLKHLGYS 191
           +GN Q +KK+AA+ AAE ALW LK++GY+
Sbjct: 115 FGNPQYKKKIAAEQAAEAALWYLKNVGYT 142

The following BLAST results are available for this feature:

BLAST of Moc08g10310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153429.1	1.5e-108	100.00	uncharacterized protein LOC111020939 isoform X1 [Momordica charantia]	[more]
XP_022153430.1	9.1e-77	100.00	uncharacterized protein LOC111020939 isoform X2 [Momordica charantia]	[more]
XP_038878793.1	4.5e-68	70.85	ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida]	[more]
XP_038878769.1	4.5e-68	70.85	ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 rib...	[more]
XP_038878786.1	3.6e-65	70.83	ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida]	[more]

BLAST of Moc08g10310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7LFZ6	2.9e-25	52.17	Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]
P84634	4.9e-25	57.95	Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2	[more]
Q9M8N2	1.3e-17	28.13	Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1	[more]

BLAST of Moc08g10310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJ12	7.5e-109	100.00	uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DGU1	4.4e-77	100.00	uncharacterized protein LOC111020939 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0KYI7	3.0e-65	66.50	DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 S...	[more]
A0A5D3E0Z9	2.5e-64	64.88	Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BXK0	2.5e-64	64.88	uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Moc08g10310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20320.1	3.5e-26	57.95	dicer-like 4	[more]
AT5G20320.2	3.5e-26	57.95	dicer-like 4	[more]
AT1G80650.1	9.3e-19	28.13	RNAse THREE-like protein 1	[more]
AT4G00420.2	1.7e-17	49.44	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]
AT4G00420.3	1.7e-17	49.44	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 111..185 e-value: 1.9E-9 score: 47.4
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 110..186 score: 10.12038
None	No IPR available	PFAM	PF14709	DND1_DSRM	coord: 111..185 e-value: 1.2E-12 score: 48.0
None	No IPR available	GENE3D	3.30.160.20		coord: 117..186 e-value: 1.4E-21 score: 78.8
None	No IPR available	PANTHER	PTHR14950:SF67	DOUBLE-STRANDED RNA-BINDING DOMAIN (DSRBD)-CONTAINING PROTEIN	coord: 14..190
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 14..190
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 111..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g10310.1	Moc08g10310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090501	RNA phosphodiester bond hydrolysis
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding