Moc08g10280 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g10280
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Defective in cullin neddylation protein
Location	chr8: 7654847 .. 7658297 (-)
RNA-Seq Expression	Moc08g10280
Synteny	Moc08g10280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGCGCTCTGCTACGAGGAAAACCGGTCAACCCAATTCAACTTCCGTTAATTCTTCCGCAGTGGATCTCTTTCGTTCCGGTACCTGATCCTTTTTTTTTTCTTTCCACAATTCGAATTCTTCTACTTGGAATTCTCACCATGGAAATCGATCATCGTGTTATGTATATATCTTGGTTTGTTGCTTGCCGTCGCCATGTGCATGGTTTAATTTTGATCAATTACTCCCACGATGCCTTTGTCTAAGGAGATTTTTATCATTTTTGGATGTTATGTTAGCATCTTGCTATGCTAATGCTTTAGTTAGTCTTTAAATAGAGTGAGATTTCAGTGGGATGAATAGCTCTAGTATTTGGTTGATTATTTGACGGCTATCAAATTTGGAAATTTCGAGCTTATTATACAGATTGTTACATACGTGTACTTAAGCACCAGTAAGAGTTAATTCAAGATGACATCGAGCGAGTCGATATCACTAAAGCAAAAGGCAGGAGTTGTAGAGTTAGAGATTGAAAGCAAAAGGCTCCCAACATGCAAACTACTTTTATTCTTTGTTTTTACGAATTTTCAAATTTAATTGGGTAGTAAACTTTGCTGTGGGGACTGGGGATATGGAAGCCCCTCTTTACATTTTGTTCAGTCTTGCTGGAATAAACTTCATCATCTGCTGATATTTTGTTATTTATTATATTGCTTGAGTGGTTTTTTTTTTTTGGGTAAAATTATAATGTGTCAATTCTCATGAACTTAATATTCTAAATTTATTCAGCCTCAAGTAGAGCGAGCACAAAAGAGTTGGAACGGATTGATAGTCTATATTATTCATATGCCAATAGCTCTTCTGGTTTGATCGAGTAAGTAAACTTTTGGTCCATGGAAGTATCTCAATTTTCAACATTGCTAGTTAGTTTTGCGTTGTACCAATATTTTCTTTATTCAGTATTATACTGTTTAAATTCCAAGAAACATTCAAGTTAAGTAATGAATCTGTTTTCTTTTTATGGTTGATGTCTCATGCCTTACTCTTGTATAACAGTATCCTTCTACTTCAAAAATTTCCATCCATTTTCCTTTTATGATCAAGGATGTTGTTTTCCTTATCTTTAAGGAGATTTCTAAATACTTGTATACATAAAATATGTATGGTATGCAGTACTGACTATTTCTGTAACAGTCCAGAAGGAATTGAAACTCTTTGTTCAGACATGGAAGTGAATCACACTGATGTGAGGATCTTAATGCTTGCATGGTAAGTTGTACTTGGATTGATATAATAGCTCAATATGTTTGTGTATATACCTCATTGCCCAGAAACTAATGGATTGCACATACTAGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGTAAGAATATTTACTCTCTTGTGGGCCTCGGAATATTATAAATTATATGGCAGATCTTCTTTCTAGTATTTCTGTCCTTTTATTTTCTGCATTCTACATCATTTAATCCTTTCATCTCTATTATCTATTTAGTATGGTAATCCATGAAGAGTATATTTTACCTATATCATTTATGTTCCCCAGCTCCAATTTATTCAGAAACTTCTGTTGACAAGATTTATAAATTCTTTATATATTTTCTTTTCCTCTCTTGTTTACTTTACTCTTCATTCTCTTTGCATGATGGCCCATCTTATTATCTATTCGTAATTGCTCTGTGATGACAAGATGAAGCATTTAGTCCCTTCTACCCTCTTTTCGGTAAATTTATTTGATAGAATCTAACCTATTGCATATTTGATAGGAAGAATGGCGAAGAGGCCTCAAATCCCTGAGGGCTGACACTGTTAGTAAACTAAAGAAGGCGCTACCAGATCTGGAGAAAGAGGTACAATACGTGTCCTGTTAAAATTTCCAAAATAAAGAAGCAAATATAGTTGGTGCAAATTGCTTAACTTTCCTGCTTAAAGTTTTTTGATTATCGATTCCTCACATTTTGAGAAGGTAAGGAGGCCATCAAACTTTGGGGACTTCTATTCTTATGCATTTCAGTATTGTTTAACAGGTAAACAAATTGTGTTTAGTGTGTTTTAATCTTGAGGCTTGGTTCATTTTCTTCTATTCTCTATTGTAATTTGACCTTTACATTTTCTTTATTCCATGATATTTTTCTTAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGCATATGTGAGTTGCTGGATCTTGTTTTAGGGTCTCAATTTCATTCCCAAGTCAATGCGTTTGTTGAATACTTGAAGGTGAGTGCAGCATCATGTTTGTTGTTAATTTACCACATTTATCCATGGGCATGGCTTATTTGCCTCTGCAATTAACTCCTGCACTGCCTTATAGTACTTCTAATTCTAGATATTTAATAGTAGCACGTATAAGAGAGGTATGTTATTGAGTTATGGCCCTGCCTCTCTCTAGCCAAGTGTTGATAAAAGGGACTTTACCATATTTCTTCTGGTAGCCGTAGCCATCTAGGTAATTTGAAAGACATGCTGTAGAAAGTGTAGATTGTATAGTATCAAAATTTTCTCGCGTAGGATTTCATTTATTTGGGTTATTGATTGTTTAATCCCGTAGTTGCTATCTTCCTCTCGTCCAAAAGGCAGAATATAAAGTTCTTATTCGCTCGTATTATGTATGCAGTTACAAAGTGACTATAAGGTTATTAACATGGATCAATGGATGGGCTTCTTTCGATTCTGCAATGAGGTACATTCATTCTCTCTCTCTCTCTCCTAAAGCACATTTATTTTATTATTATTATTATTATTTTTTTTTGCTTCCCATCTATTGCACTACCAGTGGAGAACAGTGAGAAAATTTTAGAAGTAAATAGTTGCTAACGGAATTTACTATAACATGCTGTGAAGAAAATAGTATTCAAAGGTAGGTATTTAAGGTCCCGTTTGGAAAGCATTTTGTTTAGCTTTTTCATGAATTTCTTTGTTTCCTCAAATTCCAATCAAATTTTAACTAAGAAAAGTAGTTTTTATTTTTGGAATTTGGCTAAAAACGGAAATGTATTTTTTAAGAAAGATAAAAACATGCCAAGGAAGTTGTGAGCAAACAAGTATAATTTTATGGAGAACTAGTATCTAAGTATGTGAATTCTTTTGTTTTCTAATTGGTCTATCAAATGGGTATTTGGATGGTGCAGTGGTTGAAATAGTTCCTAGAATTTCTCCTACTTTACAAACTTCCAAGCATATAGAAAATCATAAGGAAAAGTGCAGTATGATGCAACAATAATTTCTCTATCCACCTTCAATTCATTTGTTTGTTTCATCAGTATATGGTATTGATGTCCCTCAATTCAAGTTTGTGACACTTGATGATCATTATGCAGATAAGCTATCCTGACCTGAGCAATTACGATTCGGAGCTTGCTTGGCCTTTGATCCTCGACAATTTTGTCGAATGGTTACATGCAAAACAGAGTTAA

mRNA sequence

ATGCGGCGCTCTGCTACGAGGAAAACCGGTCAACCCAATTCAACTTCCGTTAATTCTTCCGCAGTGGATCTCTTTCGTTCCGCCTCAAGTAGAGCGAGCACAAAAGAGTTGGAACGGATTGATAGTCTATATTATTCATATGCCAATAGCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAACTCTTTGTTCAGACATGGAAGTGAATCACACTGATGTGAGGATCTTAATGCTTGCATGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGAAGAATGGCGAAGAGGCCTCAAATCCCTGAGGGCTGACACTGTTAGTAAACTAAAGAAGGCGCTACCAGATCTGGAGAAAGAGGTAAGGAGGCCATCAAACTTTGGGGACTTCTATTCTTATGCATTTCAGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGCATATGTGAGTTGCTGGATCTTGTTTTAGGGTCTCAATTTCATTCCCAAGTCAATGCGTTTGTTGAATACTTGAAGTTACAAAGTGACTATAAGGTTATTAACATGGATCAATGGATGGGCTTCTTTCGATTCTGCAATGAGATAAGCTATCCTGACCTGAGCAATTACGATTCGGAGCTTGCTTGGCCTTTGATCCTCGACAATTTTGTCGAATGGTTACATGCAAAACAGAGTTAA

Coding sequence (CDS)

ATGCGGCGCTCTGCTACGAGGAAAACCGGTCAACCCAATTCAACTTCCGTTAATTCTTCCGCAGTGGATCTCTTTCGTTCCGCCTCAAGTAGAGCGAGCACAAAAGAGTTGGAACGGATTGATAGTCTATATTATTCATATGCCAATAGCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAACTCTTTGTTCAGACATGGAAGTGAATCACACTGATGTGAGGATCTTAATGCTTGCATGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGAAGAATGGCGAAGAGGCCTCAAATCCCTGAGGGCTGACACTGTTAGTAAACTAAAGAAGGCGCTACCAGATCTGGAGAAAGAGGTAAGGAGGCCATCAAACTTTGGGGACTTCTATTCTTATGCATTTCAGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGCATATGTGAGTTGCTGGATCTTGTTTTAGGGTCTCAATTTCATTCCCAAGTCAATGCGTTTGTTGAATACTTGAAGTTACAAAGTGACTATAAGGTTATTAACATGGATCAATGGATGGGCTTCTTTCGATTCTGCAATGAGATAAGCTATCCTGACCTGAGCAATTACGATTCGGAGCTTGCTTGGCCTTTGATCCTCGACAATTTTGTCGAATGGTTACATGCAAAACAGAGTTAA

Protein sequence

MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGIETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS

Homology

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BLAST of Moc08g10280 vs. NCBI nr
Match: XP_022153426.1 (DCN1-like protein 4 [Momordica charantia])

HSP 1 Score: 463.4 bits (1191), Expect = 1.2e-126
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS
Sbjct: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 230

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BLAST of Moc08g10280 vs. NCBI nr
Match: XP_008454171.1 (PREDICTED: DCN1-like protein 4 [Cucumis melo] >XP_016901452.1 PREDICTED: DCN1-like protein 4 [Cucumis melo])

HSP 1 Score: 438.7 bits (1127), Expect = 3.0e-119
Identity = 216/230 (93.91%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSL+YSYAN +SGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLFYSYANPTSGLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLK+Q+
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWL AKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLQAKQN 230

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BLAST of Moc08g10280 vs. NCBI nr
Match: XP_038876378.1 (DCN1-like protein 4 [Benincasa hispida] >XP_038876439.1 DCN1-like protein 4 [Benincasa hispida])

HSP 1 Score: 436.8 bits (1122), Expect = 1.2e-118
Identity = 216/230 (93.91%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERIDSL+YSYAN+SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANASSGLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E+LCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFH QV+AFVEYLK+QS
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVDAFVEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMGFFRFCNEIS+ DLSNYDSELAWPLILDNFVEWL AKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFSDLSNYDSELAWPLILDNFVEWLQAKQN 230

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BLAST of Moc08g10280 vs. NCBI nr
Match: XP_004152174.1 (DCN1-like protein 5 [Cucumis sativus] >KAE8649068.1 hypothetical protein Csa_014584 [Cucumis sativus])

HSP 1 Score: 434.9 bits (1117), Expect = 4.4e-118
Identity = 214/230 (93.04%), Postives = 224/230 (97.39%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERIDSL+YSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFV+YLK+Q+
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMGFFRFCNEISYPDL NYDSELAWPLILDNFVEWL AKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 230

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BLAST of Moc08g10280 vs. NCBI nr
Match: XP_023523784.1 (DCN1-like protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.0 bits (1107), Expect = 6.3e-117
Identity = 210/228 (92.11%), Postives = 224/228 (98.25%), Query Frame = 0


Query: 3   RSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGIET 62
           R  TRKTGQPNSTSVNSSAVDLFRSASS+ASTKELERI+SL+YSYAN+SSGLIDPEGIET
Sbjct: 2   RRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIET 61

Query: 63  LCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEV 122
           LCSDMEV+HTDVRILMLAWKMK+EKQGYFNLEEWRRGLKSL+ADTVSKLKKALPDLEKEV
Sbjct: 62  LCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEV 121

Query: 123 RRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDY 182
           RRPSNF DFYSYAF+YCLTEEKQKSIDIESICELL+LVLGSQFH+QVN+FVEYLK+QSDY
Sbjct: 122 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDY 181

Query: 183 KVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           KV+NMDQWMGFFRFCNEIS+PDLSNYDSELAWPLILDNFVEWL AKQS
Sbjct: 182 KVVNMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

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BLAST of Moc08g10280 vs. ExPASy Swiss-Prot
Match: Q8CCA0 (DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 4.5e-33
Identity = 83/205 (40.49%), Postives = 118/205 (57.56%), Query Frame = 0


Query: 29  SSRASTKE----LERIDSLYYSYANSSSGLIDPEGIETLCSDMEVNHTDVRILMLAWKMK 88
           S+R  T+E     +R    +Y YA +    + PEG+E  C D+ V   +V +L+LAWK+ 
Sbjct: 91  STRIKTEEEAFSSKRCLEWFYEYAGTEDA-VGPEGMEKFCEDIGVEPENVVMLVLAWKLD 150

Query: 89  SEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEVRRPSNFGDFYSYAFQYCLTEEK 148
           ++  GYF L+EW +G+ SL+ DT  KL+  L  L   +   +NF   Y YAF +   E+ 
Sbjct: 151 AQNMGYFTLQEWLKGMTSLQCDTTEKLRTTLDYLRSLLNDTTNFKLIYRYAFDFA-REKD 210

Query: 149 QKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDYKVINMDQWMGFFRFCNEISYPD 208
           Q+S+DI +   +L L+LG +       F ++L+ QS YKVIN DQW     F   IS  D
Sbjct: 211 QRSLDINTAKCMLGLLLG-KIWPLFPVFHQFLE-QSKYKVINKDQWCNVLEFSRTISL-D 270

Query: 209 LSNYDSELAWPLILDNFVEWLHAKQ 230
           LSNYD + AWP++LD FVEW   KQ
Sbjct: 271 LSNYDEDGAWPVLLDEFVEWYKDKQ 290

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BLAST of Moc08g10280 vs. ExPASy Swiss-Prot
Match: Q92564 (DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 5.9e-33
Identity = 82/205 (40.00%), Postives = 119/205 (58.05%), Query Frame = 0


Query: 29  SSRASTKE----LERIDSLYYSYANSSSGLIDPEGIETLCSDMEVNHTDVRILMLAWKMK 88
           S+R  T+E     +R    +Y YA +   ++ PEG+E  C D+ V   +V +L+LAWK+ 
Sbjct: 91  STRIKTEEEAFSSKRCLEWFYEYAGTDD-VVGPEGMEKFCEDIGVEPENVVMLVLAWKLD 150

Query: 89  SEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEVRRPSNFGDFYSYAFQYCLTEEK 148
           ++  GYF L+EW +G+ SL+ DT  KL+  L  L   +   +NF   Y YAF +   E+ 
Sbjct: 151 AQNMGYFTLQEWLKGMTSLQCDTTEKLRNTLDYLRSFLNDSTNFKLIYRYAFDFA-REKD 210

Query: 149 QKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDYKVINMDQWMGFFRFCNEISYPD 208
           Q+S+DI +   +L L+LG +       F ++L+ QS YKVIN DQW     F   I+  D
Sbjct: 211 QRSLDINTAKCMLGLLLG-KIWPLFPVFHQFLE-QSKYKVINKDQWCNVLEFSRTINL-D 270

Query: 209 LSNYDSELAWPLILDNFVEWLHAKQ 230
           LSNYD + AWP++LD FVEW   KQ
Sbjct: 271 LSNYDEDGAWPVLLDEFVEWYKDKQ 290

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BLAST of Moc08g10280 vs. ExPASy Swiss-Prot
Match: Q9BTE7 (DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.7e-32
Identity = 70/187 (37.43%), Postives = 113/187 (60.43%), Query Frame = 0


Query: 44  YYSYANSSSGLIDPEGIETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSL 103
           +Y YA     ++ PEG+E  C D+ V   ++ +L+LAWK+++E  G+F  EEW +G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHSQVNAFVEYLKLQSDYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVE 223
            +    + F +YL+ QS Y+V+N DQW     F   + + DLSNYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLHAKQS 231
           W   +Q+
Sbjct: 235 WQKVRQT 236

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BLAST of Moc08g10280 vs. ExPASy Swiss-Prot
Match: Q1RMX9 (DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-32
Identity = 70/187 (37.43%), Postives = 113/187 (60.43%), Query Frame = 0


Query: 44  YYSYANSSSGLIDPEGIETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSL 103
           +Y YA     ++ PEG+E  C D+ V   ++ +L+LAWK+++E  G+F  EEW +G+ SL
Sbjct: 54  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 113

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 114 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 173

Query: 164 QFHSQVNAFVEYLKLQSDYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVE 223
            +    + F +YL+ QS Y+V+N DQW     F   + + DLSNYD + AWP++LD FVE
Sbjct: 174 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 233

Query: 224 WLHAKQS 231
           W   +Q+
Sbjct: 234 WHKVRQA 235

[Close]

BLAST of Moc08g10280 vs. ExPASy Swiss-Prot
Match: Q9CXV9 (DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-32
Identity = 70/187 (37.43%), Postives = 113/187 (60.43%), Query Frame = 0


Query: 44  YYSYANSSSGLIDPEGIETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSL 103
           +Y YA     ++ PEG+E  C D+ V   ++ +L+LAWK+++E  G+F  EEW +G+ SL
Sbjct: 55  FYEYAGPDE-VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQSRFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHSQVNAFVEYLKLQSDYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVE 223
            +    + F +YL+ QS Y+V+N DQW     F   + + DLSNYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLHAKQS 231
           W   +Q+
Sbjct: 235 WQKIRQT 236

[Close]

BLAST of Moc08g10280 vs. ExPASy TrEMBL
Match: A0A6J1DKL7 (Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111020935 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 5.6e-127
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS
Sbjct: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 230

[Close]

BLAST of Moc08g10280 vs. ExPASy TrEMBL
Match: A0A1S4DZM9 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.5e-119
Identity = 216/230 (93.91%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSL+YSYAN +SGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLFYSYANPTSGLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLK+Q+
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWL AKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLQAKQN 230

[Close]

BLAST of Moc08g10280 vs. ExPASy TrEMBL
Match: A0A0A0KYL5 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G006480 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.1e-118
Identity = 214/230 (93.04%), Postives = 224/230 (97.39%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERIDSL+YSYAN SSGLIDPEGI
Sbjct: 92  MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 151

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 152 ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 211

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFV+YLK+Q+
Sbjct: 212 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 271

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMGFFRFCNEISYPDL NYDSELAWPLILDNFVEWL AKQ+
Sbjct: 272 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 321

[Close]

BLAST of Moc08g10280 vs. ExPASy TrEMBL
Match: A0A6J1G9W7 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111452134 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.2e-117
Identity = 210/228 (92.11%), Postives = 224/228 (98.25%), Query Frame = 0


Query: 3   RSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGIET 62
           R  TRKTGQPNSTSVNSSAVDLFRSASS+ASTKELERI+SL+YSYAN+SSGLIDPEGIET
Sbjct: 2   RRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINSLFYSYANASSGLIDPEGIET 61

Query: 63  LCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEV 122
           LCSDMEV+HTDVRILMLAWKMK+EKQGYFNLEEWRRGLKSL+ADTVSKLKKALPDLEKEV
Sbjct: 62  LCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEV 121

Query: 123 RRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDY 182
           RRPSNF DFYSYAF+YCLTEEKQKSIDIESICELL+LVLGSQFH+QVN+FVEYLK+QSDY
Sbjct: 122 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDY 181

Query: 183 KVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           KVINMDQWMGFFRFCNEI++PDLSNYDSELAWPLILDNFVEWL AKQS
Sbjct: 182 KVINMDQWMGFFRFCNEINFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

[Close]

BLAST of Moc08g10280 vs. ExPASy TrEMBL
Match: A0A6J1KCA5 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111492580 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.2e-116
Identity = 210/228 (92.11%), Postives = 223/228 (97.81%), Query Frame = 0


Query: 3   RSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGIET 62
           R  TRKTGQPNSTSVNSSAVDLFRSASS+ASTKELERI+ L+YSYAN+SSGLIDPEGIET
Sbjct: 2   RRTTRKTGQPNSTSVNSSAVDLFRSASSKASTKELERINILFYSYANASSGLIDPEGIET 61

Query: 63  LCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEV 122
           LCSDMEV+HTDVRILMLAWKMK+EKQGYFNLEEWRRGLKSL+ADTVSKLKKALPDLEKEV
Sbjct: 62  LCSDMEVDHTDVRILMLAWKMKAEKQGYFNLEEWRRGLKSLKADTVSKLKKALPDLEKEV 121

Query: 123 RRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDY 182
           RRPSNF DFYSYAF+YCLTEEKQKSIDIESICELL+LVLGSQFH+QVN+FVEYLK+QSDY
Sbjct: 122 RRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLNLVLGSQFHAQVNSFVEYLKIQSDY 181

Query: 183 KVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           KVINMDQWMGFFRFCNEIS+PDLSNYDSELAWPLILDNFVEWL AKQS
Sbjct: 182 KVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 229

[Close]

BLAST of Moc08g10280 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 350.5 bits (898), Expect = 1.0e-96
Identity = 166/230 (72.17%), Postives = 202/230 (87.83%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS KE++RID L+  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV+HTD+RILMLAWKMK+EKQGYF  EEWRRGLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F +QV+ FVEYLK+Q+
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMG +RFCNEIS+PD+ +Y+ ELAWPLILDNFVEW+  KQ+
Sbjct: 181 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 227

[Close]

BLAST of Moc08g10280 vs. TAIR 10
Match: AT1G15860.2 (Domain of unknown function (DUF298) )

HSP 1 Score: 350.5 bits (898), Expect = 1.0e-96
Identity = 166/230 (72.17%), Postives = 202/230 (87.83%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS KE++RID L+  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV+HTD+RILMLAWKMK+EKQGYF  EEWRRGLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F +QV+ FVEYLK+Q+
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 180

Query: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
           DYKVINMDQWMG +RFCNEIS+PD+ +Y+ ELAWPLILDNFVEW+  KQ+
Sbjct: 181 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 227

[Close]

BLAST of Moc08g10280 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 342.8 bits (878), Expect = 2.1e-94
Identity = 166/239 (69.46%), Postives = 202/239 (84.52%), Query Frame = 0


Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS KE++RID L+  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV+HTD+RILMLAWKMK+EKQGYF  EEWRRGLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLK--- 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F +QV+ FVEYLK   
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKVWI 180

Query: 181 ------LQSDYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 231
                 +Q+DYKVINMDQWMG +RFCNEIS+PD+ +Y+ ELAWPLILDNFVEW+  KQ+
Sbjct: 181 TQKSHIIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 236

[Close]

BLAST of Moc08g10280 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-27
Identity = 68/210 (32.38%), Postives = 120/210 (57.14%), Query Frame = 0


Query: 20  SAVDLFRS-ASSRASTKELERIDSLYYSYANSSSGLIDPEGIETLCSDMEVNHTDVRILM 79
           +A D+F S    R++  E+ R++ LY  Y +  S +I  EGI  LC+D+EV   D+  L+
Sbjct: 39  AAFDVFYSQPQPRSNAAEVRRLEELYNRYKDPYSDMILAEGISVLCNDLEVEPQDIVTLV 98

Query: 80  LAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEKEVRRPSNFGDFYSYAFQY 139
           L+W M +     F+ +E+  GL++L  D++ KL++ LP +  E++    F + Y++AF +
Sbjct: 99  LSWHMNAATACEFSRQEFISGLQALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGW 158

Query: 140 CLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDYKVINMDQWMGFFRFCN 199
              E+ QKS+ +++   +  L+   +    V  + ++L+ + + K I+ D W     F  
Sbjct: 159 A-KEKGQKSLALDTAIGMWQLLFAEREWPLVTHWCDFLQDRHN-KAISKDTWAQLLEFSR 218

Query: 200 EISYPDLSNYDSELAWPLILDNFVEWLHAK 229
            +  P LSNYD+E AWP ++D FVE+L+ K
Sbjct: 219 MVD-PVLSNYDAEGAWPYLIDEFVEYLYDK 245

[Close]

BLAST of Moc08g10280 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 51.6 bits (122), Expect = 9.7e-07
Identity = 31/102 (30.39%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 126 SNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQSDYKVI 185
           + F  FY + F  C  E  QK+I I        LVL  +F   +N + ++++    +  I
Sbjct: 84  TEFTCFYDFVFFMC-RENGQKNITISRAITAWKLVLAGRFR-LLNRWCDFIEKNQRHN-I 143

Query: 186 NMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHA 228
           + D W     F +   + +L  YDSE AWP+++D+FVE +++
Sbjct: 144 SEDTWQQVLAF-SRCVHENLEGYDSEGAWPVLIDDFVEHMYS 181

The following BLAST results are available for this feature:

BLAST of Moc08g10280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153426.1	1.2e-126	100.00	DCN1-like protein 4 [Momordica charantia]	[more]
XP_008454171.1	3.0e-119	93.91	PREDICTED: DCN1-like protein 4 [Cucumis melo] >XP_016901452.1 PREDICTED: DCN1-li...	[more]
XP_038876378.1	1.2e-118	93.91	DCN1-like protein 4 [Benincasa hispida] >XP_038876439.1 DCN1-like protein 4 [Ben...	[more]
XP_004152174.1	4.4e-118	93.04	DCN1-like protein 5 [Cucumis sativus] >KAE8649068.1 hypothetical protein Csa_014...	[more]
XP_023523784.1	6.3e-117	92.11	DCN1-like protein 4 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc08g10280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8CCA0	4.5e-33	40.49	DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1	[more]
Q92564	5.9e-33	40.00	DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2	[more]
Q9BTE7	1.7e-32	37.43	DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1	[more]
Q1RMX9	2.2e-32	37.43	DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1	[more]
Q9CXV9	2.2e-32	37.43	DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1	[more]

BLAST of Moc08g10280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DKL7	5.6e-127	100.00	Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A1S4DZM9	1.5e-119	93.91	Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 ...	[more]
A0A0A0KYL5	2.1e-118	93.04	Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G0064...	[more]
A0A6J1G9W7	5.2e-117	92.11	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KCA5	1.2e-116	92.11	Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]

BLAST of Moc08g10280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15860.1	1.0e-96	72.17	Domain of unknown function (DUF298)	[more]
AT1G15860.2	1.0e-96	72.17	Domain of unknown function (DUF298)	[more]
AT1G15860.3	2.1e-94	69.46	Domain of unknown function (DUF298)	[more]
AT3G12760.1	1.3e-27	32.38	CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP...	[more]
AT3G28970.1	9.7e-07	30.39	Domain of unknown function (DUF298)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005176	Potentiating neddylation domain	PFAM	PF03556	Cullin_binding	coord: 110..224 e-value: 1.5E-27 score: 96.4
IPR005176	Potentiating neddylation domain	PROSITE	PS51229	DCUN1	coord: 35..226 score: 42.321583
None	No IPR available	GENE3D	1.10.238.10		coord: 33..123 e-value: 3.2E-28 score: 99.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR12281:SF12	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 1..229
IPR042460	DCN1-like, PONY binding domain	GENE3D	1.10.238.200	Cullin, PONY binding domain	coord: 125..230 e-value: 1.2E-28 score: 101.5
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 1..229
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 34..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g10280.1	Moc08g10280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding