Moc08g10220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g10220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	aquaporin NIP6-1
Location	chr8: 7617437 .. 7618390 (+)
RNA-Seq Expression	Moc08g10220
Synteny	Moc08g10220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACACAGATCAGGAAGCTCCATCCACGCCGGGGACACCTGGAGCTCCTCTTTTTGAAGGGATCAGGGCGGAACACCATGGAAATGGAAATGGTAAAAGATCTCTTCTCAAGAACTGCATCTCTTTCAATGCCGATGATTGGACCAATGAAGAAAGAGGCTTATCCTCCAAGGTCTCTTGTTCATTGTCGCCTCCTCCTATCTCGCTTGCAAGAAAGGTAATTCTCGAGAGATGAAAGAGCAAAATTTAAGATCTATTTAGTAATATCACAGTTTCTTATTTCTTATTTTCTGTTTATCATTCTTCGTAGAAGGAAAAAAAACTACATTTAGCAGTAACATCTTATTTAACTTTTTCACTAAAAGATGTACCTCAGTGTCAGTGATCGTTCCTTATTTCAAGTTCTCTAAAATTTTAAATGAAAATAAAAAGTTACATGAAATCTATCTTTTCTCAAATAAAATATTCAAAATTATTATATTATATATATTTGTTAATATTTGTGATGCATTTAATAAATATAGAATAGTAAAAGTATATTTTTATTAACATGATTGGAAAATTCAAGCAGATAATTAAAAGAAAACGTTTCTAAAAAACAATAAATAGGAAAAAGAAATTAGACAGCAATATCACTCAAAACAAGAAACAAAAACATTATCAAATGGTCTCTTAACCCCACATCTCGTTATACCTAAAGGTGGGAGCAGAGTTCATCAGTACTCTGATGCTCATCTTTGCTGGAACCGCCACGGCCATTGTAAACCAGAAGACACAAGGAACCGAAACACTCATCGGCCTAGCAGCCTCGACCGGGCTGGCGGTGATGATTGTCATCTCGTCGACTGGCCACATCTCTGGAGCCCATCTGAACCCGGCCATCACCATTGCTTTTGCAGCACTAAAGCACTTTCCATGGAAGCATGTAGGCGCCATGATCTCCATGCACTGA

mRNA sequence

ATGGACACAGATCAGGAAGCTCCATCCACGCCGGGGACACCTGGAGCTCCTCTTTTTGAAGGGATCAGGGCGGAACACCATGGAAATGGAAATGGTAAAAGATCTCTTCTCAAGAACTGCATCTCTTTCAATGCCGATGATTGGACCAATGAAGAAAGAGGCTTATCCTCCAAGGTCTCTTGTTCATTGTCGCCTCCTCCTATCTCGCTTGCAAGAAAGGTGGGAGCAGAGTTCATCAGTACTCTGATGCTCATCTTTGCTGGAACCGCCACGGCCATTGTAAACCAGAAGACACAAGGAACCGAAACACTCATCGGCCTAGCAGCCTCGACCGGGCTGGCGGTGATGATTGTCATCTCGTCGACTGGCCACATCTCTGGAGCCCATCTGAACCCGGCCATCACCATTGCTTTTGCAGCACTAAAGCACTTTCCATGGAAGCATGTAGGCGCCATGATCTCCATGCACTGA

Coding sequence (CDS)

ATGGACACAGATCAGGAAGCTCCATCCACGCCGGGGACACCTGGAGCTCCTCTTTTTGAAGGGATCAGGGCGGAACACCATGGAAATGGAAATGGTAAAAGATCTCTTCTCAAGAACTGCATCTCTTTCAATGCCGATGATTGGACCAATGAAGAAAGAGGCTTATCCTCCAAGGTCTCTTGTTCATTGTCGCCTCCTCCTATCTCGCTTGCAAGAAAGGTGGGAGCAGAGTTCATCAGTACTCTGATGCTCATCTTTGCTGGAACCGCCACGGCCATTGTAAACCAGAAGACACAAGGAACCGAAACACTCATCGGCCTAGCAGCCTCGACCGGGCTGGCGGTGATGATTGTCATCTCGTCGACTGGCCACATCTCTGGAGCCCATCTGAACCCGGCCATCACCATTGCTTTTGCAGCACTAAAGCACTTTCCATGGAAGCATGTAGGCGCCATGATCTCCATGCACTGA

Protein sequence

MDTDQEAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHISGAHLNPAITIAFAALKHFPWKHVGAMISMH

Homology

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BLAST of Moc08g10220 vs. NCBI nr
Match: XP_022153403.1 (aquaporin NIP6-1 [Momordica charantia])

HSP 1 Score: 295.8 bits (756), Expect = 2.2e-76
Identity = 150/153 (98.04%), Postives = 150/153 (98.04%), Query Frame = 0


Query: 1   MDTDQEAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVS 60
           MDTDQEAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVS
Sbjct: 1   MDTDQEAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVS 60

Query: 61  CSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVIS 120
           CSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVIS
Sbjct: 61  CSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVIS 120

Query: 121 STGHISGAHLNPAITIAFAALKHFPWKHVGAMI 154
           STGHISGAHLNPAITIAFAALKHFPWKHV   I
Sbjct: 121 STGHISGAHLNPAITIAFAALKHFPWKHVPVYI 153

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BLAST of Moc08g10220 vs. NCBI nr
Match: XP_038876197.1 (aquaporin NIP6-1 [Benincasa hispida])

HSP 1 Score: 218.0 bits (554), Expect = 5.7e-53
Identity = 119/157 (75.80%), Postives = 129/157 (82.17%), Query Frame = 0


Query: 1   MDTDQ--EAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADD-WTNEERGLSS 60
           MDT++    P TPGTPGAPLF  ++   HG+ NGKRSLLK+CISFN DD W  EE GLS 
Sbjct: 1   MDTEETPSTPVTPGTPGAPLFGRVKENRHGSENGKRSLLKSCISFNVDDNWGTEEGGLSK 60

Query: 61  KV-SCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVM 120
            V SCSL  PP+SLARKVGAEFI TL+LIFAGTATAIVNQKT+GTE LIGLAASTGLAVM
Sbjct: 61  LVSSCSLPYPPVSLARKVGAEFIGTLILIFAGTATAIVNQKTEGTEMLIGLAASTGLAVM 120

Query: 121 IVISSTGHISGAHLNPAITIAFAALKHFPWKHVGAMI 154
           IVI STGHISGAHLNPAITIAFAALK FPWK+V   I
Sbjct: 121 IVILSTGHISGAHLNPAITIAFAALKQFPWKYVPVYI 157

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BLAST of Moc08g10220 vs. NCBI nr
Match: XP_004152170.1 (aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [Cucumis sativus])

HSP 1 Score: 216.1 bits (549), Expect = 2.2e-52
Identity = 117/153 (76.47%), Postives = 128/153 (83.66%), Query Frame = 0


Query: 1   MDTDQ--EAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADD-WTNEERGLSS 60
           MDT++    P TPGTPGAPLF  ++   HG+ NGKRSLLK+CISFN DD W +EE GL+ 
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60

Query: 61  KV-SCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVM 120
            V SCSL   P+SLARKVGAEFI TL+LIFAGTATAIVNQKT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120

Query: 121 IVISSTGHISGAHLNPAITIAFAALKHFPWKHV 150
           IVI STGHISGAHLNPA+TIAFAALK FPWKHV
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHV 153

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BLAST of Moc08g10220 vs. NCBI nr
Match: PHT47594.1 (Aquaporin NIP3-1 [Capsicum baccatum])

HSP 1 Score: 214.9 bits (546), Expect = 4.8e-52
Identity = 111/147 (75.51%), Postives = 123/147 (83.67%), Query Frame = 0


Query: 7   APSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPP 66
           AP+TPGTPGAPLF G + E    GNGK+SLLK+C  F+ + W +EE G    VSC L PP
Sbjct: 12  APATPGTPGAPLFGGFKHERKNTGNGKKSLLKSCKCFSVEPWGSEE-GTLPAVSCMLPPP 71

Query: 67  PISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHIS 126
           P+SLARKVGAEFI TL+LIFAGTATAIVNQKTQG+ETLIGLAAS+GLAVMIVI STGHIS
Sbjct: 72  PVSLARKVGAEFIGTLILIFAGTATAIVNQKTQGSETLIGLAASSGLAVMIVILSTGHIS 131

Query: 127 GAHLNPAITIAFAALKHFPWKHVGAMI 154
           GAHLNPA+TIAFAALKHFPWKHV   I
Sbjct: 132 GAHLNPALTIAFAALKHFPWKHVPVYI 157

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BLAST of Moc08g10220 vs. NCBI nr
Match: KAF3622880.1 (Aquaporin NIP3-1 [Capsicum annuum] >KAF3665362.1 Aquaporin NIP3-1 [Capsicum annuum] >PHT88450.1 Aquaporin NIP3-1 [Capsicum annuum] >PHU24094.1 Aquaporin NIP3-1 [Capsicum chinense])

HSP 1 Score: 214.9 bits (546), Expect = 4.8e-52
Identity = 111/147 (75.51%), Postives = 123/147 (83.67%), Query Frame = 0


Query: 7   APSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPP 66
           AP+TPGTPGAPLF G + E    GNGK+SLLK+C  F+ + W +EE G    VSC L PP
Sbjct: 12  APATPGTPGAPLFGGFKHERKNTGNGKKSLLKSCKCFSVEPWGSEE-GTLPAVSCMLPPP 71

Query: 67  PISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHIS 126
           P+SLARKVGAEFI TL+LIFAGTATAIVNQKTQG+ETLIGLAAS+GLAVMIVI STGHIS
Sbjct: 72  PVSLARKVGAEFIGTLILIFAGTATAIVNQKTQGSETLIGLAASSGLAVMIVILSTGHIS 131

Query: 127 GAHLNPAITIAFAALKHFPWKHVGAMI 154
           GAHLNPA+TIAFAALKHFPWKHV   I
Sbjct: 132 GAHLNPALTIAFAALKHFPWKHVPVYI 157

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BLAST of Moc08g10220 vs. ExPASy Swiss-Prot
Match: Q9SAI4 (Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 7.0e-46
Identity = 101/154 (65.58%), Postives = 116/154 (75.32%), Query Frame = 0


Query: 3   TDQEAPSTPGTPGAPLFEGIRAEHHGNGNGK---RSLLKNCISFNADDWTNEERGLSSKV 62
           T     +TPGTPGAPLF G   + +G+ NG+   +SLLK+C  F+ D+    E G    V
Sbjct: 9   TPSTPATTPGTPGAPLFGGFEGKRNGH-NGRYTPKSLLKSCKCFSVDNEWALEDGRLPPV 68

Query: 63  SCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVI 122
           +CSL PP +SL RK+GAEF+ TL+LIFAGTATAIVNQKT G ETLIG AAS GLAVMIVI
Sbjct: 69  TCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAVMIVI 128

Query: 123 SSTGHISGAHLNPAITIAFAALKHFPWKHVGAMI 154
            STGHISGAHLNPA+TIAFAALKHFPWKHV   I
Sbjct: 129 LSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYI 161

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BLAST of Moc08g10220 vs. ExPASy Swiss-Prot
Match: Q9SV84 (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.2e-32
Identity = 77/147 (52.38%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 8   PSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPPP 67
           P TPGTPG PL  G+R +     + K +    C+      W     G           P 
Sbjct: 19  PGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPVMGSTW-----GQHDTCFTDFPSPD 78

Query: 68  ISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHISG 127
           +SL RK+GAEF+ T +LIF  TA  IVNQK  G ETLIG AA  GLAVMI+I STGHISG
Sbjct: 79  VSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISG 138

Query: 128 AHLNPAITIAFAALKHFPWKHVGAMIS 155
           AHLNP++TIAFAAL+HFPW HV A I+
Sbjct: 139 AHLNPSLTIAFAALRHFPWAHVPAYIA 160

[Close]

BLAST of Moc08g10220 vs. ExPASy Swiss-Prot
Match: Q0IWF3 (Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 3.6e-26
Identity = 71/149 (47.65%), Postives = 89/149 (59.73%), Query Frame = 0


Query: 7   APSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPP 66
           AP+TPGTP APLF G R +            K   +  A+ W     G   ++      P
Sbjct: 23  APATPGTP-APLFAGPRVDSLSYERKSMPRCKCLPAAVAEAWAPSAHGCVVEIPA----P 82

Query: 67  PISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHIS 126
            +SL RK+GAEF+ T +LIF  TA  IVNQK  G  +  G AA  GLAV  +I STGHIS
Sbjct: 83  DVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHIS 142

Query: 127 GAHLNPAITIAFAALKHFPWKHVGAMISM 156
           GAHLNP++TIAFAAL+HFPW  V A +++
Sbjct: 143 GAHLNPSLTIAFAALRHFPWLQVPAYVAV 166

[Close]

BLAST of Moc08g10220 vs. ExPASy Swiss-Prot
Match: Q9ATN1 (Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.8e-24
Identity = 71/152 (46.71%), Postives = 93/152 (61.18%), Query Frame = 0


Query: 7   APSTPGTPGAPLFEGIRAEHHGNGNGKRSLLK-NCISFNA-DDWTNEERGLSSKVS-CSL 66
           AP+TPGTP APLF             ++S+ +  C+   A + W     G+++      +
Sbjct: 12  APATPGTP-APLFSSGGPRVDSLSYERKSMPRCKCLPLPAVEGW-----GVATHTCVVEI 71

Query: 67  SPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTG 126
             P +SL RK+GAEF+ T +LIF  TA  IVNQK  G  +  G AA  GLAV  VI STG
Sbjct: 72  PAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQKYGGAISPFGNAACAGLAVATVILSTG 131

Query: 127 HISGAHLNPAITIAFAALKHFPWKHVGAMISM 156
           HISGAHLNP++TIAFAAL+HFPW  V A +++
Sbjct: 132 HISGAHLNPSLTIAFAALRHFPWLQVPAYVAV 157

[Close]

BLAST of Moc08g10220 vs. ExPASy Swiss-Prot
Match: Q0DK16 (Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 4.8e-18
Identity = 51/103 (49.51%), Postives = 66/103 (64.08%), Query Frame = 0


Query: 50  NEERGLSSKVSCSLSPP-PISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLA 109
           N E+   SK S +  P   +  A+KV AE + T  LIFAG A   VN++T GT T  G+ 
Sbjct: 31  NSEQDGGSKSSSNNHPMFSVQFAQKVIAEILGTFFLIFAGCAAVAVNKRTGGTVTFPGIC 90

Query: 110 ASTGLAVMIVISSTGHISGAHLNPAITIAFAALKHFPWKHVGA 152
            + GLAVM+++ S GHISGAHLNPA+T+AFA    FPW+ V A
Sbjct: 91  ITWGLAVMVMVYSVGHISGAHLNPAVTLAFATCGRFPWRRVPA 133

[Close]

BLAST of Moc08g10220 vs. ExPASy TrEMBL
Match: A0A6J1DHD3 (aquaporin NIP6-1 OS=Momordica charantia OX=3673 GN=LOC111020915 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.0e-76
Identity = 150/153 (98.04%), Postives = 150/153 (98.04%), Query Frame = 0


Query: 1   MDTDQEAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVS 60
           MDTDQEAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVS
Sbjct: 1   MDTDQEAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVS 60

Query: 61  CSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVIS 120
           CSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVIS
Sbjct: 61  CSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVIS 120

Query: 121 STGHISGAHLNPAITIAFAALKHFPWKHVGAMI 154
           STGHISGAHLNPAITIAFAALKHFPWKHV   I
Sbjct: 121 STGHISGAHLNPAITIAFAALKHFPWKHVPVYI 153

[Close]

BLAST of Moc08g10220 vs. ExPASy TrEMBL
Match: A0A0A0KX08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-52
Identity = 117/153 (76.47%), Postives = 128/153 (83.66%), Query Frame = 0


Query: 1   MDTDQ--EAPSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADD-WTNEERGLSS 60
           MDT++    P TPGTPGAPLF  ++   HG+ NGKRSLLK+CISFN DD W +EE GL+ 
Sbjct: 1   MDTEEAPSTPVTPGTPGAPLFGRVKENQHGSENGKRSLLKSCISFNVDDNWGSEEGGLTK 60

Query: 61  KV-SCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVM 120
            V SCSL   P+SLARKVGAEFI TL+LIFAGTATAIVNQKT GTETLIGLAASTGLAVM
Sbjct: 61  IVSSCSLPYSPVSLARKVGAEFIGTLILIFAGTATAIVNQKTGGTETLIGLAASTGLAVM 120

Query: 121 IVISSTGHISGAHLNPAITIAFAALKHFPWKHV 150
           IVI STGHISGAHLNPA+TIAFAALK FPWKHV
Sbjct: 121 IVILSTGHISGAHLNPAVTIAFAALKQFPWKHV 153

[Close]

BLAST of Moc08g10220 vs. ExPASy TrEMBL
Match: A0A2G2WQS2 (Aquaporin NIP3-1 OS=Capsicum baccatum OX=33114 GN=CQW23_11802 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.3e-52
Identity = 111/147 (75.51%), Postives = 123/147 (83.67%), Query Frame = 0


Query: 7   APSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPP 66
           AP+TPGTPGAPLF G + E    GNGK+SLLK+C  F+ + W +EE G    VSC L PP
Sbjct: 12  APATPGTPGAPLFGGFKHERKNTGNGKKSLLKSCKCFSVEPWGSEE-GTLPAVSCMLPPP 71

Query: 67  PISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHIS 126
           P+SLARKVGAEFI TL+LIFAGTATAIVNQKTQG+ETLIGLAAS+GLAVMIVI STGHIS
Sbjct: 72  PVSLARKVGAEFIGTLILIFAGTATAIVNQKTQGSETLIGLAASSGLAVMIVILSTGHIS 131

Query: 127 GAHLNPAITIAFAALKHFPWKHVGAMI 154
           GAHLNPA+TIAFAALKHFPWKHV   I
Sbjct: 132 GAHLNPALTIAFAALKHFPWKHVPVYI 157

[Close]

BLAST of Moc08g10220 vs. ExPASy TrEMBL
Match: A0A2G3CZF5 (Aquaporin NIP3-1 OS=Capsicum chinense OX=80379 GN=BC332_09201 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.3e-52
Identity = 111/147 (75.51%), Postives = 123/147 (83.67%), Query Frame = 0


Query: 7   APSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPP 66
           AP+TPGTPGAPLF G + E    GNGK+SLLK+C  F+ + W +EE G    VSC L PP
Sbjct: 12  APATPGTPGAPLFGGFKHERKNTGNGKKSLLKSCKCFSVEPWGSEE-GTLPAVSCMLPPP 71

Query: 67  PISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHIS 126
           P+SLARKVGAEFI TL+LIFAGTATAIVNQKTQG+ETLIGLAAS+GLAVMIVI STGHIS
Sbjct: 72  PVSLARKVGAEFIGTLILIFAGTATAIVNQKTQGSETLIGLAASSGLAVMIVILSTGHIS 131

Query: 127 GAHLNPAITIAFAALKHFPWKHVGAMI 154
           GAHLNPA+TIAFAALKHFPWKHV   I
Sbjct: 132 GAHLNPALTIAFAALKHFPWKHVPVYI 157

[Close]

BLAST of Moc08g10220 vs. ExPASy TrEMBL
Match: A0A2G3A2M0 (Aquaporin NIP3-1 OS=Capsicum annuum OX=4072 GN=T459_10556 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.3e-52
Identity = 111/147 (75.51%), Postives = 123/147 (83.67%), Query Frame = 0


Query: 7   APSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPP 66
           AP+TPGTPGAPLF G + E    GNGK+SLLK+C  F+ + W +EE G    VSC L PP
Sbjct: 12  APATPGTPGAPLFGGFKHERKNTGNGKKSLLKSCKCFSVEPWGSEE-GTLPAVSCMLPPP 71

Query: 67  PISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHIS 126
           P+SLARKVGAEFI TL+LIFAGTATAIVNQKTQG+ETLIGLAAS+GLAVMIVI STGHIS
Sbjct: 72  PVSLARKVGAEFIGTLILIFAGTATAIVNQKTQGSETLIGLAASSGLAVMIVILSTGHIS 131

Query: 127 GAHLNPAITIAFAALKHFPWKHVGAMI 154
           GAHLNPA+TIAFAALKHFPWKHV   I
Sbjct: 132 GAHLNPALTIAFAALKHFPWKHVPVYI 157

[Close]

BLAST of Moc08g10220 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 184.9 bits (468), Expect = 5.0e-47
Identity = 101/154 (65.58%), Postives = 116/154 (75.32%), Query Frame = 0


Query: 3   TDQEAPSTPGTPGAPLFEGIRAEHHGNGNGK---RSLLKNCISFNADDWTNEERGLSSKV 62
           T     +TPGTPGAPLF G   + +G+ NG+   +SLLK+C  F+ D+    E G    V
Sbjct: 9   TPSTPATTPGTPGAPLFGGFEGKRNGH-NGRYTPKSLLKSCKCFSVDNEWALEDGRLPPV 68

Query: 63  SCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVI 122
           +CSL PP +SL RK+GAEF+ TL+LIFAGTATAIVNQKT G ETLIG AAS GLAVMIVI
Sbjct: 69  TCSLPPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAVMIVI 128

Query: 123 SSTGHISGAHLNPAITIAFAALKHFPWKHVGAMI 154
            STGHISGAHLNPA+TIAFAALKHFPWKHV   I
Sbjct: 129 LSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYI 161

[Close]

BLAST of Moc08g10220 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 141.0 bits (354), Expect = 8.3e-34
Identity = 77/147 (52.38%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 8   PSTPGTPGAPLFEGIRAEHHGNGNGKRSLLKNCISFNADDWTNEERGLSSKVSCSLSPPP 67
           P TPGTPG PL  G+R +     + K +    C+      W     G           P 
Sbjct: 19  PGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPVMGSTW-----GQHDTCFTDFPSPD 78

Query: 68  ISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHISG 127
           +SL RK+GAEF+ T +LIF  TA  IVNQK  G ETLIG AA  GLAVMI+I STGHISG
Sbjct: 79  VSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISG 138

Query: 128 AHLNPAITIAFAALKHFPWKHVGAMIS 155
           AHLNP++TIAFAAL+HFPW HV A I+
Sbjct: 139 AHLNPSLTIAFAALRHFPWAHVPAYIA 160

[Close]

BLAST of Moc08g10220 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 82.4 bits (202), Expect = 3.5e-16
Identity = 48/132 (36.36%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 29  NGNGKRSLLKNCISFNADDWTNEERGLSS-------KVSCSLSPPPISLARKVGAEFIST 88
           +GNG  +  +  +  N  D    ++ +         K   SL    +   +K+ AEF+ T
Sbjct: 5   SGNGYGNAREEVVMVNLKDEVEHQQEMEDIHNPRPLKKQDSLLSVSVPFLQKLIAEFLGT 64

Query: 89  LMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTGHISGAHLNPAITIAFAAL 148
             L+F G A+ +VN +     TL G+A   GL +M++I S GHISGAH+NPA+TIAFA+ 
Sbjct: 65  YFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIMVLIYSLGHISGAHINPAVTIAFASC 124

Query: 149 KHFPWKHVGAMI 154
             FP K V A +
Sbjct: 125 GRFPLKQVPAYV 136

[Close]

BLAST of Moc08g10220 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 82.4 bits (202), Expect = 3.5e-16
Identity = 44/99 (44.44%), Postives = 59/99 (59.60%), Query Frame = 0


Query: 51  EERGLSSKVSCSLSPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAAS 110
           + +G      CS SP  + L +K+ AE I T  +IF+G    +VN    GT T  G+  +
Sbjct: 23  DSQGGMETAICS-SPSIVCLTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVT 82

Query: 111 TGLAVMIVISSTGHISGAHLNPAITIAFAALKHFPWKHV 150
            GL VM++I STGHISGAH NPA+T+ FA  + FPW  V
Sbjct: 83  WGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQV 120

[Close]

BLAST of Moc08g10220 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 81.3 bits (199), Expect = 7.8e-16
Identity = 40/86 (46.51%), Postives = 54/86 (62.79%), Query Frame = 0


Query: 64  SPPPISLARKVGAEFISTLMLIFAGTATAIVNQKTQGTETLIGLAASTGLAVMIVISSTG 123
           SP  + L +K+ AE I T  ++F+G    +VN    GT T  G+  + GL VM++I STG
Sbjct: 35  SPSIVCLTQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTG 94

Query: 124 HISGAHLNPAITIAFAALKHFPWKHV 150
           HISGAH NPA+T+ FA  + FPW  V
Sbjct: 95  HISGAHFNPAVTVTFAIFRRFPWHQV 120

The following BLAST results are available for this feature:

BLAST of Moc08g10220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153403.1	2.2e-76	98.04	aquaporin NIP6-1 [Momordica charantia]	[more]
XP_038876197.1	5.7e-53	75.80	aquaporin NIP6-1 [Benincasa hispida]	[more]
XP_004152170.1	2.2e-52	76.47	aquaporin NIP6-1 [Cucumis sativus] >KGN52947.1 hypothetical protein Csa_015162 [...	[more]
PHT47594.1	4.8e-52	75.51	Aquaporin NIP3-1 [Capsicum baccatum]	[more]
KAF3622880.1	4.8e-52	75.51	Aquaporin NIP3-1 [Capsicum annuum] >KAF3665362.1 Aquaporin NIP3-1 [Capsicum annu...	[more]

BLAST of Moc08g10220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAI4	7.0e-46	65.58	Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1	[more]
Q9SV84	1.2e-32	52.38	Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1	[more]
Q0IWF3	3.6e-26	47.65	Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2	[more]
Q9ATN1	5.8e-24	46.71	Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1	[more]
Q0DK16	4.8e-18	49.51	Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2	[more]

BLAST of Moc08g10220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHD3	1.0e-76	98.04	aquaporin NIP6-1 OS=Momordica charantia OX=3673 GN=LOC111020915 PE=3 SV=1	[more]
A0A0A0KX08	1.1e-52	76.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007030 PE=3 SV=1	[more]
A0A2G2WQS2	2.3e-52	75.51	Aquaporin NIP3-1 OS=Capsicum baccatum OX=33114 GN=CQW23_11802 PE=3 SV=1	[more]
A0A2G3CZF5	2.3e-52	75.51	Aquaporin NIP3-1 OS=Capsicum chinense OX=80379 GN=BC332_09201 PE=3 SV=1	[more]
A0A2G3A2M0	2.3e-52	75.51	Aquaporin NIP3-1 OS=Capsicum annuum OX=4072 GN=T459_10556 PE=3 SV=1	[more]

BLAST of Moc08g10220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80760.1	5.0e-47	65.58	NOD26-like intrinsic protein 6;1	[more]
AT4G10380.1	8.3e-34	52.38	NOD26-like intrinsic protein 5;1	[more]
AT4G19030.1	3.5e-16	36.36	NOD26-like major intrinsic protein 1	[more]
AT5G37820.1	3.5e-16	44.44	NOD26-like intrinsic protein 4;2	[more]
AT5G37810.1	7.8e-16	46.51	NOD26-like intrinsic protein 4;1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 72..91 score: 37.49 coord: 111..135 score: 47.02
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 69..149 e-value: 6.1E-18 score: 65.4
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 36..156 e-value: 3.5E-25 score: 90.2
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 70..153
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR45724:SF41	BNAA02G36290D PROTEIN	coord: 9..153
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 9..153
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 129..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g10220.1	Moc08g10220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015267	channel activity