Moc08g10180 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g10180
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionmetal transporter Nramp6
Locationchr8: 7563981 .. 7569664 (+)
RNA-Seq ExpressionMoc08g10180
SyntenyMoc08g10180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGGAGGTGTACAGAGGAGAGAGAAAGGGGAAGGAAGGAATGGAGATGGAGGATTATATAAAGCCACAGAGAAGTCTGAAAGAGTAAGCAAAGCCGGAGCGCTGACCACCAGCTTGTCGAACCAACCATCAACACTCGCCTCTCCATATCCTCGATTAAGGTTCGTTCTTCAAACCAATTCTTCCAATTTCCAATTCCCAATTCCCAATTTCCATTTTCCATTTTCCATTTTCATTTCCGGATCACTTGCCCTGATTCTCTCTAAATCATCACAGAAAATTTAGCATACTGAGATCTGTTTTGGTTTTCCCCTCCCCCTTTCTCTGTTCTTGCTTCCTTCGGTACCTGAGTCTTTCAGCTGGCGAATGATGGAACAGAGGATCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTTCTGAATGACTCCACTCCTACCGTATTTAATTTCTTGTTTGACTAGATTATGCTTTGATCTTCTGTAATGGGGGGTGGTTAATGGTTTCTGTGTTTCTGTGTATTGTATAATCTGAGCTTTCTTTTTCGGTTCAGTTAACTTCGGGTTCCATTTCGTTCGGAAAGAGGGGGAAAGGAACAGGCACAATAAAATGGCTGCTGGAGGGCCTAGTTCTGGGCAGCCACAATTCCTTGCAAGGGCCGGAGATGAGAGCTTTTCGCACGCGCCGTTGATCGAGAACTCGGAAACTGATCAGATTGTTGTTCCAGACGTGAGGATAAATTTTTATTTTTCTGATTATCAATCGTTTCATGTTTGCAATTTATTGGATGATTAATCATTTGTGGCGCTTTGAATACGAAGATACTTTAAGTAACTTGTAGGGTGGAGCCAGAAATCTTCGTAGGAAGGGAAAATTAGAGAGAACATGTTAAAATCTTAACGTTGTAATGAAATTTTTGTTTTAATGTAAGATAAACGAGGGAGGAAAGTTCTCATTTTTTCATTCGCTCCGTCCTTGGTAACTAGTAAGATCTGAAGTATTCGCTATGAAAAGTGCTGAAGGAATTTTCAATTGAGAGCTACTATTGCGTGTTATGTGCAGAAGAAAAGCTGGAAGAACCTTTTTGCGTACATGGGACCGGGGTTTCTTGTTTCTATTGCCTATATTGATCCTGGGAATTGTAAGATTTGTAGTTTCGCTGCCAAAAAAAAGATTTGTAGTTTCTCTCTTTTACAATATTTTCAGTTTTCTAGTTTCTCTTTATTCCATTATCATTATGAATGGGATTCTGAAGACTTGGTATGGTTACTCTCAGTTGAAACTGATCTACAATCTGGAGCACAGTACAAGTATGGGGTACGTAAGTGGTTGCTTTACCTTTATCCACCAACACATCATATTGTGGTCACAGGCCACAATTTGGTATCATCTTTCAATTTTATGTCCCCCACACATCCATCTGTATGGATCAGTTTATTAAGTAATTTGTGATTTACATAACGCATTCCTTGTGGTAAATGGTATATATTCCTTCACTTGAGGTTGGAGGTTCAAATCCTCGTACCCTCACATTTGTTGTACTAAAAAAGAATTGGTTCCTATTTTTTTTCCCTTGTGGAAGCTGGAAAGTCCTGCTTCACCATTCAAATTTCACATGATAGTTATATTGCTAATGGGCCTGACAACTAACACCTCTTCTCTTGGCACAGTTGCTTTGGATCATATTAGTAGCTTCCTGTGCTGCCCTGATCATTCAATCCTTAGCAGCCAATCTAGGGGTTGTCACAGGTAAATAACTTATGCATAGGTTTTTCCTTTTCCTTTTCTCTAATTTGTGATATTTCTCTAGTTTCAAGAGTTTTTAGACATCTTTTTTCTTCTTGTTTTTTTTCGTAAGTGTAGGAAAACATTTAGCAGAGCACTGCAAAGCTGAATACCCCAGGGTACAAAATTTCATCCTATGGGTTCTTGCTGAAATTGCAATAGTTGCATGTGATATTCCTGAAGGTAAAGTGATCCTCTTCATGAGAAAGCTAAAAGTTGAGTGAAAGATACTCTTGGTCGTTGGAGAAATAAGTACAATTGTTGCATGCTTAGTCTTTTGCATTTTGTGTACTTGTTTTGGATATTTAATTTAAAATGAAGTTATTGAGATCCGTACCCGCCAGCCAATTAAAAAGGTTGAGTGGAGATTGATATTTGTAGCCGATACAAATGCTTTGTTAGTGCTAGTATCGTTAGCATCAAGGTGGAGGAGAAGGGAAATATTAGAACTACTCATCATATGCTGTAAAAGCTGATTAGGTGTCCAACCAATTGCGATGTGATTCACATTGACATGGTCAAGTGTACAAGCCAAACCAAAAAGCATGTATGGAATTAAAAGTATGCTACCAATGAAGATTCACGTTTAGATTTGCTATGGGGTGTTGTGCTCTGTATATGCTACATGACATTCATTGGTATATAATTGTTATTTGTTGTAGGTAGTTGAAGTTATTCTTACATCTCCTCCTTTCTGTTTCCAGTTATAGGAACAGCTTTTGCATTGAATATGCTCTTCAGCATTCCAGTTTGGTGTGGTGTTCTGCTGACAGGGCTTAGTACTCTACTCCTTCTTGCGCTGCAACAATATGGGGTATATTGAAATTTACTTTTTTCCGGATTTTACTTATCTTTAAGAAATTGAGATTCTAAGCCTTGCACAATATTTACATTGGGAATTGTCAAGGAGGTAGCATAATTTTAGATAACTAAACTTTAGCTACTTTAAATGCTCACCTTGACTGGGTTTATGTCCACCATGCAGATTAGAAAGCTTGAATTCTTGATTGCAATTCTTGTATTGACAATTGCAATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAACCAGATGCCGGAGAAATTCTTTATGGGCTATTTGTCCCTCAATTAAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCGTATGTCTCTGTTATATCATTTAATAACATTCATAGCCTTCATTCTCAATGTTCTAAGTCTAACTACTTGTCTGCAGACATAATCTCTTTCTTCACTCCGCACTGGTGCTCTCTAGGAAAATACCCCGATCTGTTAATGGTATCAAGGTGATGTTTCATCTTCATCAGTATAATATGTATGCTCATTTTTACTTAAAAATTTTCAAATGAGTTTAAAATCTATTTAAATTTGTACTTAACATGTAACTATCTTATAGCTTCACAGAAGTATGATGCTTTGGTTGTTGCTTTATTGTTCAGATTTTAATTCTACATTCCACCTTGCTAACATTTGCAAATTTTCTTCACAGGAAGCTTGCAGGTTTTATATGATAGAGAGTGGGTTTGCTCTTATGGTGGCCTTCCTCATTAATGTGTCGGTCATTTCTGTTAGTGGTGCAGTTTGCAATGCCCCGAATCTAAATAAGGAGGACCAAATGAGCTGCAGTGACTTGGACTTGAATAAAGCCTCATTTTTGTTGAGAGTATGTGTGATCTAGCAACTTTTGCCTATAACCTTGTTGTTTACCTCATGGGATTTGGTTCCCCACCAATGGTGCGCCGGTTTCATACAGAGGTCTTCTATACTTTTTTCAGAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTAGCATCTGGTCAGAGCTCTACCATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGTATAATGCGCTGCTCTGCTCCACTTTTCGTGCTAGCTGCTTCTTTTAGAGTTTCTGGATTCTTGTACTCATTCCATTTATATTATTGGTATAGAGTAAATCAGAAATAGCCATGCCATGGTTTTCAAATTTTCTCGATATTCATGATCATAAACATGACAGCATGGAGTGAAGAAGAAGGGAGAGAGAAATTGCCCCCAAATTTGTCTTAGATTAAAAAAATTATATATAAAAATTAAGATTGCAAAGTTCTCCAAATGTTTAGAATTGAAAGATTATATATAAAATGTTAGAAGGTAATTTGTCTCTCTGCCTTTAAACAGGACCTAATTATGGGTATTTCATAATGCTTGATTTCAAATAACTGTGTGATACTCAGTGTTTCTTTAAATCTTCCTTTTTTTTTCCTGACATATTCTTGTTTATATCACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAGGGGTTTCTTGATTTGAAACTGAGGCCATGGATCAGAAACTTTTTAACCCGAAGCTTAGCAATTGTTCCAAGTTTGATTGTTGCTATCATTGGTGGGTCTTCTGGGGCTGGGAAGTTGATCATTATTGCGTCGGTAAGATATATACCCCCCCATGAATGCGATTGAGTTTTAACTGCCAGTCAGCATATGCATCGACAATACTTCTGTCTCTGTTTTATGCACGTTTAAAAGAAAAATGTTAGTAAATTCACTTCAAAACACACATCTTCTTGAGATCGAAGTGGTTGCGATTGGAAAACACTAAACATTGCAATAGATTTCTTATTCCATATGCCTAGGATTCGATGATGTTGCACTGCTTCTATATGTCTGCTTGGATACTGCCCAAATGTTGAAGTGTTCCATAAATCACTTTGAGAGCAATTAATTTTTCACAGATTTCACTGAGGCCCCCCAAGTTTCTTGGCGTGGTTCAATTGTTGTGTGGTTCTATTGTTGCTGAAAATTGAAGCGAAAAACATTTTTGACTCCTTGCCATCTTTTTTGGCAGATGATTTTGTCATTTGAACTCCCTTTCGCTCTCGTTCCCCTTCTGAAGTTTACAAGCAGCAAGACCAAGATGGGACCACATGTTAACTCTACCGTGGTAATTTCAACTGTTTTTTTGGTTATATAATTAGAACAAATAATTTGGATTGGAAATGGTACTTCCTATCTTTCGTTAACATCATGAAAAAGATAACACCATGAATTATCCACTAAAGTAGTTCTTACCAAATGGTGCTTGATTTTTTATCTTTGACCCATAAAAAAAAGTTACTAATACTTTAACCATTAACCATTAGCTATCTTCTGGTTAGGTCATTAGACATGAATTTGAAAGCTTTTCAGAATGGTTTGTAATTTTTCCTCAAATAATGACCAAAAATGAATGTTAATTTTAAACTTCACATTAACAATCAATGTAACATTTACACCATACTAATTGCATGAACGAATCTTTATAACCAAGGAAAGGCTACAATATATATTCTGCTTGAATGCTCTCTTACTTTTTCTTTAAGGATGAACAAATGAATGTATGGCAAAATGGTTCTCACATTTGGACAACACCAAGCTGAACATCATAGTTTCTGATCATACCATTAATTTTCGTCATTTATTTTTCGAAATGCAGATTACGGTACTGACATGGATTATCGGTTTCCTCATCATGGCTATAAACATATACTACCTTATGACCCGTTTCATCCACGTGCTTCTCCACAATGATCTCCAACTCGCAGCAGTCGTCTTCATAGGGATACTTGGATTCTCAGGCATGGCATTATATTTAGCTGGAATTGCTTACCTGGTTTTCAGAAAGACCAAGGAGATCACATATCTCCTAGCACTAACAACATCAGAAAGTCTACGACTGAGCAACGAGCCGAGTAAGACATCGGGATATAGTCTCCCAAGCGAAGATACAGTAAGCATGCAGTTACCTCAAAGAACTACCAATGATGTAAACTAA

mRNA sequence

ATGGTGGGAGGTGTACAGAGGAGAGAGAAAGGGGAAGGAAGGAATGGAGATGGAGGATTATATAAAGCCACAGAGAAGTCTGAAAGAGTAAGCAAAGCCGGAGCGCTGACCACCAGCTTGTCGAACCAACCATCAACACTCGCCTCTCCATATCCTCGATTAAGAAAATTTAGCATACTGAGATCTGTTTTGGTTTTCCCCTCCCCCTTTCTCTGTTCTTGCTTCCTTCGGTACCTGATTAACTTCGGGTTCCATTTCGTTCGGAAAGAGGGGGAAAGGAACAGGCACAATAAAATGGCTGCTGGAGGGCCTAGTTCTGGGCAGCCACAATTCCTTGCAAGGGCCGGAGATGAGAGCTTTTCGCACGCGCCGTTGATCGAGAACTCGGAAACTGATCAGATTGTTGTTCCAGACAAGAAAAGCTGGAAGAACCTTTTTGCGTACATGGGACCGGGGTTTCTTGTTTCTATTGCCTATATTGATCCTGGGAATTTTGAAACTGATCTACAATCTGGAGCACAGTACAAGTATGGGTTGCTTTGGATCATATTAGTAGCTTCCTGTGCTGCCCTGATCATTCAATCCTTAGCAGCCAATCTAGGGGTTGTCACAGGAAAACATTTAGCAGAGCACTGCAAAGCTGAATACCCCAGGGTACAAAATTTCATCCTATGGGTTCTTGCTGAAATTGCAATAGTTGCATGTGATATTCCTGAAGTTATAGGAACAGCTTTTGCATTGAATATGCTCTTCAGCATTCCAGTTTGGTGTGGTGTTCTGCTGACAGGGCTTAGTACTCTACTCCTTCTTGCGCTGCAACAATATGGGATTAGAAAGCTTGAATTCTTGATTGCAATTCTTGTATTGACAATTGCAATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAACCAGATGCCGGAGAAATTCTTTATGGGCTATTTGTCCCTCAATTAAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCACATAATCTCTTTCTTCACTCCGCACTGGTGCTCTCTAGGAAAATACCCCGATCTGTTAATGGTATCAAGGAAGCTTGCAGGTTTTATATGATAGAGAGTGGGTTTGCTCTTATGGTGGCCTTCCTCATTAATGTGTCGGTCATTTCTGTTAGTGGTGCAGTTTGCAATGCCCCGAATCTAAATAAGGAGGACCAAATGAGCTGCAGTGACTTGGACTTGAATAAAGCCTCATTTTTGTTGAGAAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTAGCATCTGGTCAGAGCTCTACCATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGGGTTTCTTGATTTGAAACTGAGGCCATGGATCAGAAACTTTTTAACCCGAAGCTTAGCAATTGTTCCAAGTTTGATTGTTGCTATCATTGGTGGGTCTTCTGGGGCTGGGAAGTTGATCATTATTGCGTCGATGATTTTGTCATTTGAACTCCCTTTCGCTCTCGTTCCCCTTCTGAAGTTTACAAGCAGCAAGACCAAGATGGGACCACATGTTAACTCTACCGTGATTACGGTACTGACATGGATTATCGGTTTCCTCATCATGGCTATAAACATATACTACCTTATGACCCGTTTCATCCACGTGCTTCTCCACAATGATCTCCAACTCGCAGCAGTCGTCTTCATAGGGATACTTGGATTCTCAGGCATGGCATTATATTTAGCTGGAATTGCTTACCTGGTTTTCAGAAAGACCAAGGAGATCACATATCTCCTAGCACTAACAACATCAGAAAGTCTACGACTGAGCAACGAGCCGAGTAAGACATCGGGATATAGTCTCCCAAGCGAAGATACAGTAAGCATGCAGTTACCTCAAAGAACTACCAATGATGTAAACTAA

Coding sequence (CDS)

ATGGTGGGAGGTGTACAGAGGAGAGAGAAAGGGGAAGGAAGGAATGGAGATGGAGGATTATATAAAGCCACAGAGAAGTCTGAAAGAGTAAGCAAAGCCGGAGCGCTGACCACCAGCTTGTCGAACCAACCATCAACACTCGCCTCTCCATATCCTCGATTAAGAAAATTTAGCATACTGAGATCTGTTTTGGTTTTCCCCTCCCCCTTTCTCTGTTCTTGCTTCCTTCGGTACCTGATTAACTTCGGGTTCCATTTCGTTCGGAAAGAGGGGGAAAGGAACAGGCACAATAAAATGGCTGCTGGAGGGCCTAGTTCTGGGCAGCCACAATTCCTTGCAAGGGCCGGAGATGAGAGCTTTTCGCACGCGCCGTTGATCGAGAACTCGGAAACTGATCAGATTGTTGTTCCAGACAAGAAAAGCTGGAAGAACCTTTTTGCGTACATGGGACCGGGGTTTCTTGTTTCTATTGCCTATATTGATCCTGGGAATTTTGAAACTGATCTACAATCTGGAGCACAGTACAAGTATGGGTTGCTTTGGATCATATTAGTAGCTTCCTGTGCTGCCCTGATCATTCAATCCTTAGCAGCCAATCTAGGGGTTGTCACAGGAAAACATTTAGCAGAGCACTGCAAAGCTGAATACCCCAGGGTACAAAATTTCATCCTATGGGTTCTTGCTGAAATTGCAATAGTTGCATGTGATATTCCTGAAGTTATAGGAACAGCTTTTGCATTGAATATGCTCTTCAGCATTCCAGTTTGGTGTGGTGTTCTGCTGACAGGGCTTAGTACTCTACTCCTTCTTGCGCTGCAACAATATGGGATTAGAAAGCTTGAATTCTTGATTGCAATTCTTGTATTGACAATTGCAATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAACCAGATGCCGGAGAAATTCTTTATGGGCTATTTGTCCCTCAATTAAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCACATAATCTCTTTCTTCACTCCGCACTGGTGCTCTCTAGGAAAATACCCCGATCTGTTAATGGTATCAAGGAAGCTTGCAGGTTTTATATGATAGAGAGTGGGTTTGCTCTTATGGTGGCCTTCCTCATTAATGTGTCGGTCATTTCTGTTAGTGGTGCAGTTTGCAATGCCCCGAATCTAAATAAGGAGGACCAAATGAGCTGCAGTGACTTGGACTTGAATAAAGCCTCATTTTTGTTGAGAAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTAGCATCTGGTCAGAGCTCTACCATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGGGTTTCTTGATTTGAAACTGAGGCCATGGATCAGAAACTTTTTAACCCGAAGCTTAGCAATTGTTCCAAGTTTGATTGTTGCTATCATTGGTGGGTCTTCTGGGGCTGGGAAGTTGATCATTATTGCGTCGATGATTTTGTCATTTGAACTCCCTTTCGCTCTCGTTCCCCTTCTGAAGTTTACAAGCAGCAAGACCAAGATGGGACCACATGTTAACTCTACCGTGATTACGGTACTGACATGGATTATCGGTTTCCTCATCATGGCTATAAACATATACTACCTTATGACCCGTTTCATCCACGTGCTTCTCCACAATGATCTCCAACTCGCAGCAGTCGTCTTCATAGGGATACTTGGATTCTCAGGCATGGCATTATATTTAGCTGGAATTGCTTACCTGGTTTTCAGAAAGACCAAGGAGATCACATATCTCCTAGCACTAACAACATCAGAAAGTCTACGACTGAGCAACGAGCCGAGTAAGACATCGGGATATAGTCTCCCAAGCGAAGATACAGTAAGCATGCAGTTACCTCAAAGAACTACCAATGATGTAAACTAA

Protein sequence

MVGGVQRREKGEGRNGDGGLYKATEKSERVSKAGALTTSLSNQPSTLASPYPRLRKFSILRSVLVFPSPFLCSCFLRYLINFGFHFVRKEGERNRHNKMAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGMALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQRTTNDVN
Homology
BLAST of Moc08g10180 vs. NCBI nr
Match: XP_022153441.1 (metal transporter Nramp6 [Momordica charantia])

HSP 1 Score: 1030.0 bits (2662), Expect = 8.6e-297
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 158
           MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 159 YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 218
           YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 120

Query: 219 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 278
           VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 279 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 338
           KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 339 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 398
           NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN
Sbjct: 241 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 300

Query: 399 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 458
           KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 459 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 518
           KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK
Sbjct: 361 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 519 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 578
           TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM
Sbjct: 421 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 480

Query: 579 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQRTTN 638
           ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQRTTN
Sbjct: 481 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQRTTN 540

Query: 639 DVN 642
           DVN
Sbjct: 541 DVN 543

BLAST of Moc08g10180 vs. NCBI nr
Match: XP_038905496.1 (metal transporter Nramp6-like [Benincasa hispida] >XP_038905497.1 metal transporter Nramp6-like [Benincasa hispida])

HSP 1 Score: 979.9 bits (2532), Expect = 1.0e-281
Identity = 517/545 (94.86%), Postives = 527/545 (96.70%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 158
           MAAGG  SGQPQFL RAGDESFSHAPLIEN ETDQI+VPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDESFSHAPLIENPETDQIIVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 159 YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 218
           YIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKHLAEHCKAEYP+
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 219 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 278
            QNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 279 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 338
           KLEFLIA LVLTIAICFFLELGYAKPD GEILYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 339 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 398
           NLFLHSALVLSRKIPRS++GIKEACRFYMIESGFALMVAFLINVSVISVSGAVCN+P+LN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 399 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 458
           KEDQMSC+DLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 459 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 518
           KL PWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 519 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 578
           TKMGPHVNST ITVLTWIIGFLIMAINIYYLMTRFIHV+LHNDLQLA VVFIGILGFSGM
Sbjct: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLMTRFIHVILHNDLQLAVVVFIGILGFSGM 480

Query: 579 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQ--RT 638
           ALYLAGIAYLV RKTKEIT+LLALTT ES RLSNEPSKTSGY LP+ED VSMQLPQ  RT
Sbjct: 481 ALYLAGIAYLVLRKTKEITHLLALTTEESRRLSNEPSKTSGYGLPNEDIVSMQLPQRIRT 540

Query: 639 TNDVN 642
           TNDVN
Sbjct: 541 TNDVN 545

BLAST of Moc08g10180 vs. NCBI nr
Match: TYK27020.1 (metal transporter Nramp6 [Cucumis melo var. makuwa])

HSP 1 Score: 979.5 bits (2531), Expect = 1.3e-281
Identity = 515/556 (92.63%), Postives = 533/556 (95.86%), Query Frame = 0

Query: 88  RKEGERNRHNKMAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFA 147
           +KEG+  R+NKMAAGG  SGQPQFL RAGD+SFSHAPLIEN ETDQI+VPDK+SWKNLFA
Sbjct: 14  KKEGKNKRNNKMAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFA 73

Query: 148 YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKH 207
           YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKH
Sbjct: 74  YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH 133

Query: 208 LAEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTL 267
           LAEHCKAEYP+ QNFILWVLAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTL
Sbjct: 134 LAEHCKAEYPKAQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTL 193

Query: 268 LLLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLA 327
           LLLALQQYGIRKLEFLIA LVLTIAICFFLELGYAKPD GEI YGLFVPQLKGSGATGLA
Sbjct: 194 LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA 253

Query: 328 ISLLGAMVMPHNLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISV 387
           ISLLGAMVMPHNLFLHSALVLSRKIPRS++GIKEACRFYMIESGFALMVAFLINVSVISV
Sbjct: 254 ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV 313

Query: 388 SGAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 447
           SGAVCNAP+LNKEDQMSC+DLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA
Sbjct: 314 SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 373

Query: 448 GQYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA 507
           GQYVMQGFLDLKL PWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA
Sbjct: 374 GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA 433

Query: 508 LVPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAV 567
           LVPLLKFTSSK KMGPHVNST ITVLTWIIGFLIMAINIYYLM+RFIHVLLHN+L LAAV
Sbjct: 434 LVPLLKFTSSKAKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAV 493

Query: 568 VFIGILGFSGMALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDT 627
           VFIGILGFSG+ALYLAGIAYLV RKTKEI++LLALTT ES RLSN+PSKTSGY LP+ED 
Sbjct: 494 VFIGILGFSGVALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDI 553

Query: 628 VSMQLPQ--RTTNDVN 642
           VSMQLPQ  RTTNDVN
Sbjct: 554 VSMQLPQRIRTTNDVN 569

BLAST of Moc08g10180 vs. NCBI nr
Match: KAA0037704.1 (metal transporter Nramp6 [Cucumis melo var. makuwa])

HSP 1 Score: 966.1 bits (2496), Expect = 1.5e-277
Identity = 509/545 (93.39%), Postives = 524/545 (96.15%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 158
           MAAGG  SGQPQFL RAGD+SFSHAPLIEN ETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 60

Query: 159 YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 218
           YIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKHLAEHCKAEYP+
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 219 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 278
            QNFILWVLAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 279 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 338
           KLEFLIA LVLTIAICFFLELGYAKPD GEI YGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 339 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 398
           NLFLHSALVLSRKIPRS++GIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAP+LN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 300

Query: 399 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 458
           KEDQMSC+DLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 459 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 518
           KL PWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 519 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 578
            KMGPHVNST ITVLTWIIGFLIMAINIYYLM+RFIHVLLHN+L LAAVVFIGILGFSG+
Sbjct: 421 AKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAVVFIGILGFSGV 480

Query: 579 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQ--RT 638
           ALYLAGIAYLV RKTKEI++LLALTT ES RLSN+PSKTSGY LP+ED VSMQLPQ  RT
Sbjct: 481 ALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDIVSMQLPQRIRT 540

Query: 639 TNDVN 642
           TNDVN
Sbjct: 541 TNDVN 545

BLAST of Moc08g10180 vs. NCBI nr
Match: XP_023523752.1 (metal transporter Nramp6-like [Cucurbita pepo subsp. pepo] >XP_023523753.1 metal transporter Nramp6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 962.6 bits (2487), Expect = 1.7e-276
Identity = 500/545 (91.74%), Postives = 527/545 (96.70%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 158
           MAAGGP+SGQPQF +RAGDESFSHAPLIEN+ETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGPNSGQPQFFSRAGDESFSHAPLIENTETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 159 YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 218
           YIDPGNFETDLQSGAQYKY LLWIIL+ASCAAL+IQSLAANLGVVTGKHLAEHCKAEYP+
Sbjct: 61  YIDPGNFETDLQSGAQYKYELLWIILLASCAALVIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 219 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 278
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIP+WCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPIWCGVLLTGLSTLLLLALQQYGIR 180

Query: 279 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 338
           KLEFLIA LVLTIAICFFLELGYA+PD GEI YGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAQPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 339 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 398
           NLFLHSALVLSRKIPRS+ GIKEACRFYMIESGFAL+VAF INV+VISVSGAVCN+P+LN
Sbjct: 241 NLFLHSALVLSRKIPRSIPGIKEACRFYMIESGFALLVAFFINVAVISVSGAVCNSPDLN 300

Query: 399 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 458
           KEDQMSCSDLDLN+ASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCSDLDLNEASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 459 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 518
           KL PWIRNFLTRSLAIVPSLIVAIIGG SGAGKLIII+SMILSFELPFAL+PLLKFTSSK
Sbjct: 361 KLMPWIRNFLTRSLAIVPSLIVAIIGGPSGAGKLIIISSMILSFELPFALIPLLKFTSSK 420

Query: 519 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 578
           TKMGPHVNS  IT+LTWIIGFLIMAINIYYL+TRF+HVL+H+DLQLAAV+FIG+LGFSGM
Sbjct: 421 TKMGPHVNSLAITILTWIIGFLIMAINIYYLITRFVHVLIHSDLQLAAVIFIGLLGFSGM 480

Query: 579 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQ--RT 638
           ALYLAGIAYLV RKTKEIT+LLALTT ES RLS EPSKTS Y+LPSE+TVSMQLPQ  RT
Sbjct: 481 ALYLAGIAYLVLRKTKEITHLLALTTEESERLSIEPSKTSAYNLPSEETVSMQLPQRIRT 540

Query: 639 TNDVN 642
           TNDVN
Sbjct: 541 TNDVN 545

BLAST of Moc08g10180 vs. ExPASy Swiss-Prot
Match: Q9SAH8 (Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 8.8e-228
Identity = 420/545 (77.06%), Postives = 479/545 (87.89%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARA-GDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSI 158
           MAA G  SG+ QF++ + G+ SFS++PLIENS+++QI+V +KKSWKN FAY+GPGFLVSI
Sbjct: 1   MAATG--SGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSI 60

Query: 159 AYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYP 218
           AYIDPGNFETDLQ+GA YKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAE C+AEY 
Sbjct: 61  AYIDPGNFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYS 120

Query: 219 RVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGI 278
           +V NF+LWV+AEIA+VACDIPEVIGTAFALNMLFSIPVW GVLLTGLSTL+LLALQ+YG+
Sbjct: 121 KVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGV 180

Query: 279 RKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMP 338
           RKLEFLIA LV TIAICFF+EL Y+KPD GE+L+GLFVPQLKG+GATGLAISLLGAMVMP
Sbjct: 181 RKLEFLIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMP 240

Query: 339 HNLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNL 398
           HNLFLHSALVLSRKIPRS +GIKEACRFY+IESG ALMVAFLINVSVISVSGAVCNAPNL
Sbjct: 241 HNLFLHSALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNL 300

Query: 399 NKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 458
           + ED+ +C DLDLNKASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD
Sbjct: 301 SPEDRANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 360

Query: 459 LKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSS 518
           L+L PW+RN LTR LAI+PSLIVA+IGGS+GAGKLIIIASMILSFELPFALVPLLKFTS 
Sbjct: 361 LRLEPWLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSC 420

Query: 519 KTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSG 578
           KTKMG HVN   IT LTW+IG LIM INIYYL++ FI +L+H+ ++L  VVF GILGF+G
Sbjct: 421 KTKMGSHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAG 480

Query: 579 MALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQR-T 638
           +ALYLA IAYLVFRK +  T LL    S+++            +LP +D V+MQLP R +
Sbjct: 481 IALYLAAIAYLVFRKNRVATSLLISRDSQNVE-----------TLPRQDIVNMQLPCRVS 532

Query: 639 TNDVN 642
           T+DV+
Sbjct: 541 TSDVD 532

BLAST of Moc08g10180 vs. ExPASy Swiss-Prot
Match: Q9S9N8 (Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2)

HSP 1 Score: 783.5 bits (2022), Expect = 1.8e-225
Identity = 410/523 (78.39%), Postives = 464/523 (88.72%), Query Frame = 0

Query: 113 ARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSG 172
           A   + S S++PLIENS+++QI+VP+KKSWKN F+Y+GPGFLVSIAYIDPGNFETDLQSG
Sbjct: 6   ASGSNRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSG 65

Query: 173 AQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPRVQNFILWVLAEIAI 232
           AQYKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAEHC+AEY +V NF+LWV+AEIA+
Sbjct: 66  AQYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAV 125

Query: 233 VACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAILVLTIA 292
           VACDIPEVIGTAFALNMLF+IPVW GVLLTGLSTL+LLALQQYGIRKLEFLIA LV TIA
Sbjct: 126 VACDIPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIA 185

Query: 293 ICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI 352
           +CFF+EL Y+KPD  E+LYGLFVPQLKG+GATGLAISLLGAMVMPHNLFLHSALVLSRKI
Sbjct: 186 LCFFVELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKI 245

Query: 353 PRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLNKEDQMSCSDLDLNK 412
           PRSV GIKEACR+Y+IESG ALMVAFLINVSVISVSGAVCNA +L+ ED+ SC DLDLNK
Sbjct: 246 PRSVTGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNK 305

Query: 413 ASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLRPWIRNFLTRSL 472
           ASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L PW+RNFLTR L
Sbjct: 306 ASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCL 365

Query: 473 AIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGPHVNSTVITV 532
           AI+PSLIVA+IGGS+GAGKLIIIASMILSFELPFALVPLLKFTSSKTKMG H NS VI+ 
Sbjct: 366 AIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISS 425

Query: 533 LTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGMALYLAGIAYLVFRK 592
           +TWIIG LIM INIYYL++ FI +LLH+ + L A+VF+G+LGFSG+A YLA I+YLV RK
Sbjct: 426 VTWIIGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRK 485

Query: 593 TKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQR 636
            +E       +++  L  SN  ++    +LP ED  +MQLP R
Sbjct: 486 NRE------SSSTHFLDFSNSQTEE---TLPREDIANMQLPNR 519

BLAST of Moc08g10180 vs. ExPASy Swiss-Prot
Match: Q653V6 (Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.8e-217
Identity = 397/544 (72.98%), Postives = 460/544 (84.56%), Query Frame = 0

Query: 106 SGQPQFLA---RAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDP 165
           S QPQF++   R    +    PLI++ + DQIV+P+K SWKNLF+Y+GPGFLVSIAYIDP
Sbjct: 8   SSQPQFISSVERNNQSNGPGTPLIDSIDVDQIVIPEKNSWKNLFSYIGPGFLVSIAYIDP 67

Query: 166 GNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPRVQNF 225
           GNFETDLQ+GAQYKY LLWIIL+ASCAALIIQSLAA LGVVTGKHLAEHC+AEYP+  NF
Sbjct: 68  GNFETDLQAGAQYKYELLWIILIASCAALIIQSLAARLGVVTGKHLAEHCRAEYPKATNF 127

Query: 226 ILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEF 285
           ILW+LAE+A+VACDIPEVIGTAFALNMLF IPVWCGVL+TGLSTL+LL LQQYG+RKLEF
Sbjct: 128 ILWILAELAVVACDIPEVIGTAFALNMLFKIPVWCGVLITGLSTLMLLLLQQYGVRKLEF 187

Query: 286 LIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPHNLFL 345
           LIAILV  IA CF +ELGY+KP++ E++ GLFVP+LKG+GATGLAISLLGAMVMPHNLFL
Sbjct: 188 LIAILVSLIATCFLVELGYSKPNSSEVVRGLFVPELKGNGATGLAISLLGAMVMPHNLFL 247

Query: 346 HSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLNKEDQ 405
           HSALVLSRK+PRSV+GIKEACRFYMIES FAL +AFLIN+S+ISVSGAVC + NL+ EDQ
Sbjct: 248 HSALVLSRKVPRSVHGIKEACRFYMIESAFALTIAFLINISIISVSGAVCGSDNLSPEDQ 307

Query: 406 MSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLRP 465
           M+CSDLDLNKASFLL+NVLG WSSKLFA+ALLASGQSSTITGTYAGQYVMQGFLDL++ P
Sbjct: 308 MNCSDLDLNKASFLLKNVLGNWSSKLFAVALLASGQSSTITGTYAGQYVMQGFLDLRMTP 367

Query: 466 WIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMG 525
           WIRN LTRSLAI+PSLIV+IIGGSS AG+LIIIASMILSFELPFALVPLLKFTSS+TKMG
Sbjct: 368 WIRNLLTRSLAILPSLIVSIIGGSSAAGQLIIIASMILSFELPFALVPLLKFTSSRTKMG 427

Query: 526 PHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGMALYL 585
            H NS  I+V+TW IG  I+ IN Y+L+T F+ +LLHN L   + VF GI GF GM +Y+
Sbjct: 428 QHTNSKAISVITWGIGSFIVVINTYFLITSFVKLLLHNGLSTVSQVFSGIFGFLGMLIYM 487

Query: 586 AGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYS----LPSEDTVSMQLP-QRTT 642
           A I YLVFRK ++ T L  L    ++R+    + T G      LP ED  SMQLP QRT 
Sbjct: 488 AAILYLVFRKNRKAT-LPLLEGDSTVRIVGRDTATEGEGSLGHLPREDISSMQLPQQRTA 547

BLAST of Moc08g10180 vs. ExPASy Swiss-Prot
Match: Q8H4H5 (Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 2.5e-166
Identity = 296/481 (61.54%), Postives = 379/481 (78.79%), Query Frame = 0

Query: 119 SFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYG 178
           S +H    +  + D  ++  + +WK   A++GPGF+VS+AY+DPGN ETDLQ+GA ++Y 
Sbjct: 18  SAAHDQDAKKLDADDQLLMKEPAWKRFLAHVGPGFMVSLAYLDPGNLETDLQAGANHRYE 77

Query: 179 LLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPRVQNFILWVLAEIAIVACDIP 238
           LLW+IL+    ALIIQSLAANLGVVTG+HLAE CK+EYP+     LW+LAE+A++A DIP
Sbjct: 78  LLWVILIGLIFALIIQSLAANLGVVTGRHLAEICKSEYPKFVKIFLWLLAELAVIAADIP 137

Query: 239 EVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAILVLTIAICFFLE 298
           EVIGTAFA N+LF IPVW GVL+TG STLLLL LQ+YG+RKLEFLI++LV  +A CFF E
Sbjct: 138 EVIGTAFAFNILFHIPVWVGVLITGTSTLLLLGLQKYGVRKLEFLISMLVFVMAACFFGE 197

Query: 299 LGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVNG 358
           L   KP A E++ GLF+P+L G GAT  AI+LLGA+VMPHNLFLHSALVLSRK P SV G
Sbjct: 198 LSIVKPPAKEVMKGLFIPRLNGDGATADAIALLGALVMPHNLFLHSALVLSRKTPASVRG 257

Query: 359 IKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLNKEDQMSCSDLDLNKASFLLR 418
           IK+ CRF++ ESGFAL VA LIN++V+SVSG  C++ NL++ED   C++L L+ +SFLL+
Sbjct: 258 IKDGCRFFLYESGFALFVALLINIAVVSVSGTACSSANLSQEDADKCANLSLDTSSFLLK 317

Query: 419 NVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLRPWIRNFLTRSLAIVPSL 478
           NVLGK S+ ++ +ALLASGQSSTITGTYAGQY+MQGFLD+++R W+RN +TR++AI PSL
Sbjct: 318 NVLGKSSAIVYGVALLASGQSSTITGTYAGQYIMQGFLDIRMRKWLRNLMTRTIAIAPSL 377

Query: 479 IVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGPHVNSTVITVLTWIIG 538
           IV+IIGGS GAG+LIIIASMILSFELPFAL+PLLKF+SSK+KMGPH NS  I V +W +G
Sbjct: 378 IVSIIGGSRGAGRLIIIASMILSFELPFALIPLLKFSSSKSKMGPHKNSIYIIVFSWFLG 437

Query: 539 FLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGMALYLAGIAYLVFRKTKEITY 598
            LI+ IN+Y+L T F+  L+HNDL   A V +G   F  M +Y+  + YL  RK   +T+
Sbjct: 438 LLIIGINMYFLSTSFVGWLIHNDLPKYANVLVGAAVFPFMLVYIVAVVYLTIRKDSVVTF 497

Query: 599 L 600
           +
Sbjct: 498 V 498

BLAST of Moc08g10180 vs. ExPASy Swiss-Prot
Match: Q0D7E4 (Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 4.2e-161
Identity = 288/452 (63.72%), Postives = 361/452 (79.87%), Query Frame = 0

Query: 141 SWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANL 200
           +WK   +++GPGF+V +AY+DPGN ETDLQ+GA +KY LLW+IL+    ALIIQSL+ANL
Sbjct: 31  AWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILIGLIFALIIQSLSANL 90

Query: 201 GVVTGKHLAEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVL 260
           GVVTG+HLAE CK EYP      LW+LAE+A++A DIPEVIGT FA N+LF IPVW GVL
Sbjct: 91  GVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGFAFNLLFHIPVWTGVL 150

Query: 261 LTGLSTLLLLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKG 320
           + G STLLLL LQ+YG+RKLE ++A+LV  +A CFF+E+   KP   E+L GLF+P+L G
Sbjct: 151 IAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPPVNEVLQGLFIPRLSG 210

Query: 321 SGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLI 380
            GATG +I+LLGA+VMPHNLFLHSALVLSR  P S  G+K+ CRF++ ESG AL VA L+
Sbjct: 211 PGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRFFLFESGIALFVALLV 270

Query: 381 NVSVISVSGAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSS 440
           N+++ISVSG VCNA NL+ ED + CSDL L+ +SFLLRNVLGK S+ ++ +ALLASGQSS
Sbjct: 271 NIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSSATVYGVALLASGQSS 330

Query: 441 TITGTYAGQYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMIL 500
           TITGTYAGQYVMQGFLD+K++ W+RN +TRS+AIVPSLIV+IIGGSSGAG+LI+IASMIL
Sbjct: 331 TITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGGSSGAGRLIVIASMIL 390

Query: 501 SFELPFALVPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHN 560
           SFELPFAL+PLLKF+SS  KMG + NS  I   +W++GF+I+ INIY+L T+ +  +LHN
Sbjct: 391 SFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGINIYFLSTKLVGWILHN 450

Query: 561 DLQLAAVVFIGILGFSGMALYLAGIAYLVFRK 593
            L   A V IGI+ F  M LY+  + YL FRK
Sbjct: 451 ALPTFANVLIGIVLFPLMLLYVVAVIYLTFRK 482

BLAST of Moc08g10180 vs. ExPASy TrEMBL
Match: A0A6J1DKN3 (metal transporter Nramp6 OS=Momordica charantia OX=3673 GN=LOC111020946 PE=3 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 4.2e-297
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 158
           MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 159 YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 218
           YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 120

Query: 219 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 278
           VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 279 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 338
           KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 339 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 398
           NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN
Sbjct: 241 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 300

Query: 399 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 458
           KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 459 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 518
           KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK
Sbjct: 361 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 519 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 578
           TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM
Sbjct: 421 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 480

Query: 579 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQRTTN 638
           ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQRTTN
Sbjct: 481 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQRTTN 540

Query: 639 DVN 642
           DVN
Sbjct: 541 DVN 543

BLAST of Moc08g10180 vs. ExPASy TrEMBL
Match: A0A5D3DUP0 (Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00300 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 6.5e-282
Identity = 515/556 (92.63%), Postives = 533/556 (95.86%), Query Frame = 0

Query: 88  RKEGERNRHNKMAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFA 147
           +KEG+  R+NKMAAGG  SGQPQFL RAGD+SFSHAPLIEN ETDQI+VPDK+SWKNLFA
Sbjct: 14  KKEGKNKRNNKMAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFA 73

Query: 148 YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKH 207
           YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKH
Sbjct: 74  YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH 133

Query: 208 LAEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTL 267
           LAEHCKAEYP+ QNFILWVLAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTL
Sbjct: 134 LAEHCKAEYPKAQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTL 193

Query: 268 LLLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLA 327
           LLLALQQYGIRKLEFLIA LVLTIAICFFLELGYAKPD GEI YGLFVPQLKGSGATGLA
Sbjct: 194 LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA 253

Query: 328 ISLLGAMVMPHNLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISV 387
           ISLLGAMVMPHNLFLHSALVLSRKIPRS++GIKEACRFYMIESGFALMVAFLINVSVISV
Sbjct: 254 ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV 313

Query: 388 SGAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 447
           SGAVCNAP+LNKEDQMSC+DLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA
Sbjct: 314 SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 373

Query: 448 GQYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA 507
           GQYVMQGFLDLKL PWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA
Sbjct: 374 GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA 433

Query: 508 LVPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAV 567
           LVPLLKFTSSK KMGPHVNST ITVLTWIIGFLIMAINIYYLM+RFIHVLLHN+L LAAV
Sbjct: 434 LVPLLKFTSSKAKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAV 493

Query: 568 VFIGILGFSGMALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDT 627
           VFIGILGFSG+ALYLAGIAYLV RKTKEI++LLALTT ES RLSN+PSKTSGY LP+ED 
Sbjct: 494 VFIGILGFSGVALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDI 553

Query: 628 VSMQLPQ--RTTNDVN 642
           VSMQLPQ  RTTNDVN
Sbjct: 554 VSMQLPQRIRTTNDVN 569

BLAST of Moc08g10180 vs. ExPASy TrEMBL
Match: A0A5A7T2K9 (Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1593G00280 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 7.4e-278
Identity = 509/545 (93.39%), Postives = 524/545 (96.15%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 158
           MAAGG  SGQPQFL RAGD+SFSHAPLIEN ETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 60

Query: 159 YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 218
           YIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKHLAEHCKAEYP+
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 219 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 278
            QNFILWVLAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 279 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 338
           KLEFLIA LVLTIAICFFLELGYAKPD GEI YGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 339 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 398
           NLFLHSALVLSRKIPRS++GIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAP+LN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 300

Query: 399 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 458
           KEDQMSC+DLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 459 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 518
           KL PWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 519 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 578
            KMGPHVNST ITVLTWIIGFLIMAINIYYLM+RFIHVLLHN+L LAAVVFIGILGFSG+
Sbjct: 421 AKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAVVFIGILGFSGV 480

Query: 579 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQ--RT 638
           ALYLAGIAYLV RKTKEI++LLALTT ES RLSN+PSKTSGY LP+ED VSMQLPQ  RT
Sbjct: 481 ALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDIVSMQLPQRIRT 540

Query: 639 TNDVN 642
           TNDVN
Sbjct: 541 TNDVN 545

BLAST of Moc08g10180 vs. ExPASy TrEMBL
Match: A0A0A0LD68 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G714870 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.8e-277
Identity = 509/555 (91.71%), Postives = 527/555 (94.95%), Query Frame = 0

Query: 89  KEGERNRHNKMAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAY 148
           KEG   R+NKMAAGG  SGQPQFL RAGDESFSHAPLIEN ETDQI+VPDKKSWKNLFAY
Sbjct: 21  KEGRHKRNNKMAAGGSGSGQPQFLVRAGDESFSHAPLIENPETDQIIVPDKKSWKNLFAY 80

Query: 149 MGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHL 208
           MGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVAS ++LII SLAANLGVVTGKHL
Sbjct: 81  MGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFSSLIIHSLAANLGVVTGKHL 140

Query: 209 AEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLL 268
           AEHCKAEYP+ QNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLL
Sbjct: 141 AEHCKAEYPKAQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLL 200

Query: 269 LLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAI 328
           LLALQQYGIRKLEFLIA LVLTIAICFFLELGYAKPD GEI YGLFVPQLKGSGATGLAI
Sbjct: 201 LLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAI 260

Query: 329 SLLGAMVMPHNLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVS 388
           SLLGAMVMPHNLFLHSALVLSRKIPRS++GIKEACRFYMIESGFALMVAFLINVSVISVS
Sbjct: 261 SLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVS 320

Query: 389 GAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAG 448
           GAVC++P+LNKEDQMSC+DLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAG
Sbjct: 321 GAVCSSPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAG 380

Query: 449 QYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFAL 508
           QYVMQGFLDLKL PW+RNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFAL
Sbjct: 381 QYVMQGFLDLKLTPWVRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFAL 440

Query: 509 VPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVV 568
           VPLLKFTSSK KMGPHVNST ITVLTWIIGFLIMAINIYYLM+RFIHVLLHNDL LA V+
Sbjct: 441 VPLLKFTSSKAKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNDLHLAVVI 500

Query: 569 FIGILGFSGMALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTV 628
            IGILGFSG+ALYLAGIAYLV RKTKEI++LLALT  ES RLSNE SKTSGY LP+ED V
Sbjct: 501 LIGILGFSGVALYLAGIAYLVLRKTKEISHLLALTAEESRRLSNELSKTSGYDLPNED-V 560

Query: 629 SMQLPQ--RTTNDVN 642
           SMQLPQ  RTTNDVN
Sbjct: 561 SMQLPQRIRTTNDVN 574

BLAST of Moc08g10180 vs. ExPASy TrEMBL
Match: A0A6J1GBE7 (metal transporter Nramp6-like OS=Cucurbita moschata OX=3662 GN=LOC111452625 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 3.1e-276
Identity = 499/545 (91.56%), Postives = 527/545 (96.70%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 158
           MAAGGP+SGQPQF +RAGDESFSHAPLIEN+ETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGPNSGQPQFFSRAGDESFSHAPLIENTETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 159 YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 218
           YIDPGNFETDLQSGAQYKY LLWIIL+ASCAAL+IQSLAANLGVVTGKHLAEHCKAEYP+
Sbjct: 61  YIDPGNFETDLQSGAQYKYELLWIILLASCAALVIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 219 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 278
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIP+WCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPIWCGVLLTGLSTLLLLALQQYGIR 180

Query: 279 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 338
           KLEFLIA LVLTIAICFFLELGYA+PD GEI YGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAQPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 339 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 398
           NLFLHSALVLSRKIPRS+ GIKEACRFYMIESGFAL+VAF INV+VISVSGAVCN+P+LN
Sbjct: 241 NLFLHSALVLSRKIPRSIPGIKEACRFYMIESGFALLVAFFINVAVISVSGAVCNSPDLN 300

Query: 399 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 458
           KEDQMSCSDLDLN+ASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCSDLDLNEASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 459 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 518
           KL PWIRNFLTRSLAIVPSLIVAIIGG SGAGKLIII+SMILSFELPFAL+PLLKFTSS+
Sbjct: 361 KLMPWIRNFLTRSLAIVPSLIVAIIGGPSGAGKLIIISSMILSFELPFALIPLLKFTSSE 420

Query: 519 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 578
           TKMGPHVNS  IT+LTWIIGFLIMAINIYYL+TRF+HVL+H+DLQLAAV+FIGILGFSGM
Sbjct: 421 TKMGPHVNSLAITILTWIIGFLIMAINIYYLITRFVHVLIHSDLQLAAVIFIGILGFSGM 480

Query: 579 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQ--RT 638
           ALYLAGIAYLV RKTKE+T+LLALTT ES RLS EPSKTS Y+LPSE+TVSMQLPQ  RT
Sbjct: 481 ALYLAGIAYLVLRKTKEMTHLLALTTEESERLSIEPSKTSAYNLPSEETVSMQLPQRIRT 540

Query: 639 TNDVN 642
           TNDVN
Sbjct: 541 TNDVN 545

BLAST of Moc08g10180 vs. TAIR 10
Match: AT1G80830.1 (natural resistance-associated macrophage protein 1 )

HSP 1 Score: 791.2 bits (2042), Expect = 6.3e-229
Identity = 420/545 (77.06%), Postives = 479/545 (87.89%), Query Frame = 0

Query: 99  MAAGGPSSGQPQFLARA-GDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSI 158
           MAA G  SG+ QF++ + G+ SFS++PLIENS+++QI+V +KKSWKN FAY+GPGFLVSI
Sbjct: 1   MAATG--SGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSI 60

Query: 159 AYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYP 218
           AYIDPGNFETDLQ+GA YKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAE C+AEY 
Sbjct: 61  AYIDPGNFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYS 120

Query: 219 RVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGI 278
           +V NF+LWV+AEIA+VACDIPEVIGTAFALNMLFSIPVW GVLLTGLSTL+LLALQ+YG+
Sbjct: 121 KVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGV 180

Query: 279 RKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMP 338
           RKLEFLIA LV TIAICFF+EL Y+KPD GE+L+GLFVPQLKG+GATGLAISLLGAMVMP
Sbjct: 181 RKLEFLIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMP 240

Query: 339 HNLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNL 398
           HNLFLHSALVLSRKIPRS +GIKEACRFY+IESG ALMVAFLINVSVISVSGAVCNAPNL
Sbjct: 241 HNLFLHSALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNL 300

Query: 399 NKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 458
           + ED+ +C DLDLNKASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD
Sbjct: 301 SPEDRANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 360

Query: 459 LKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSS 518
           L+L PW+RN LTR LAI+PSLIVA+IGGS+GAGKLIIIASMILSFELPFALVPLLKFTS 
Sbjct: 361 LRLEPWLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSC 420

Query: 519 KTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSG 578
           KTKMG HVN   IT LTW+IG LIM INIYYL++ FI +L+H+ ++L  VVF GILGF+G
Sbjct: 421 KTKMGSHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAG 480

Query: 579 MALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQR-T 638
           +ALYLA IAYLVFRK +  T LL    S+++            +LP +D V+MQLP R +
Sbjct: 481 IALYLAAIAYLVFRKNRVATSLLISRDSQNVE-----------TLPRQDIVNMQLPCRVS 532

Query: 639 TNDVN 642
           T+DV+
Sbjct: 541 TSDVD 532

BLAST of Moc08g10180 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 783.5 bits (2022), Expect = 1.3e-226
Identity = 410/523 (78.39%), Postives = 464/523 (88.72%), Query Frame = 0

Query: 113 ARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSG 172
           A   + S S++PLIENS+++QI+VP+KKSWKN F+Y+GPGFLVSIAYIDPGNFETDLQSG
Sbjct: 6   ASGSNRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSG 65

Query: 173 AQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPRVQNFILWVLAEIAI 232
           AQYKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAEHC+AEY +V NF+LWV+AEIA+
Sbjct: 66  AQYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAV 125

Query: 233 VACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAILVLTIA 292
           VACDIPEVIGTAFALNMLF+IPVW GVLLTGLSTL+LLALQQYGIRKLEFLIA LV TIA
Sbjct: 126 VACDIPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIA 185

Query: 293 ICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI 352
           +CFF+EL Y+KPD  E+LYGLFVPQLKG+GATGLAISLLGAMVMPHNLFLHSALVLSRKI
Sbjct: 186 LCFFVELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKI 245

Query: 353 PRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLNKEDQMSCSDLDLNK 412
           PRSV GIKEACR+Y+IESG ALMVAFLINVSVISVSGAVCNA +L+ ED+ SC DLDLNK
Sbjct: 246 PRSVTGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNK 305

Query: 413 ASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLRPWIRNFLTRSL 472
           ASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L PW+RNFLTR L
Sbjct: 306 ASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCL 365

Query: 473 AIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGPHVNSTVITV 532
           AI+PSLIVA+IGGS+GAGKLIIIASMILSFELPFALVPLLKFTSSKTKMG H NS VI+ 
Sbjct: 366 AIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISS 425

Query: 533 LTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGMALYLAGIAYLVFRK 592
           +TWIIG LIM INIYYL++ FI +LLH+ + L A+VF+G+LGFSG+A YLA I+YLV RK
Sbjct: 426 VTWIIGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRK 485

Query: 593 TKEITYLLALTTSESLRLSNEPSKTSGYSLPSEDTVSMQLPQR 636
            +E       +++  L  SN  ++    +LP ED  +MQLP R
Sbjct: 486 NRE------SSSTHFLDFSNSQTEE---TLPREDIANMQLPNR 519

BLAST of Moc08g10180 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 318.2 bits (814), Expect = 1.6e-86
Identity = 192/461 (41.65%), Postives = 287/461 (62.26%), Query Frame = 0

Query: 139 KKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAA 198
           K SWK L+ + GPGFL+SIA++DPGN E+DLQ+GA   Y L+W+++ A+   L+IQ L+A
Sbjct: 46  KFSWKKLWLFTGPGFLMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAIGLLIQLLSA 105

Query: 199 NLGVVTGKHLAEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFS--IPVW 258
            LGV TG+HLAE C+ EYP     +LW++AEIA++  DI EVIG+A A+ +L +  +P+W
Sbjct: 106 RLGVATGRHLAELCREEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLW 165

Query: 259 CGVLLTGLSTLLLLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVP 318
            GV++T L   + L L+ YGIRKLE + AIL+ T+A+ F    G  KP   E+L G  VP
Sbjct: 166 AGVVITALDCFIFLFLENYGIRKLEAVFAILIATMALAFAWMFGQTKPSGTELLVGALVP 225

Query: 319 QLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSVNGIKEACRFYMIESGFALM 378
           +L  S     A+ ++G ++MPHN+FLHSALV SR++ P+    +KEA ++Y IES  AL 
Sbjct: 226 KL-SSRTIKQAVGIVGCIIMPHNVFLHSALVQSREVDPKKRFRVKEALKYYSIESTGALA 285

Query: 379 VAFLINVSVISVSGAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSK---LFAIA 438
           V+F+INV V +V         +          + L  A   L++  G        ++AI 
Sbjct: 286 VSFIINVFVTTVFAKSFYGTEI-------ADTIGLANAGQYLQDKYGGGFFPILYIWAIG 345

Query: 439 LLASGQSSTITGTYAGQYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGA--G 498
           +LA+GQSSTITGTYAGQ++M GFL+LK++ W+R  +TRS AI+P++IVA++  SS +   
Sbjct: 346 VLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALITRSCAIIPTMIVALVFDSSDSMLD 405

Query: 499 KLIIIASMILSFELPFALVPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLM 558
           +L    +++ S ++PFA++PLL   S++  MG      ++  ++WI+  L++AIN  YLM
Sbjct: 406 ELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKIQPLVQTISWIVAALVIAIN-GYLM 465

Query: 559 TRFIHVLLHNDLQLAAVVFIGILGFSGMALYLAGIAYLVFR 592
             F      N + L  V+   I        Y+  + YL+ R
Sbjct: 466 VDFFSGAATNLILLVPVIIFAI-------AYVVFVLYLISR 490

BLAST of Moc08g10180 vs. TAIR 10
Match: AT2G23150.1 (natural resistance-associated macrophage protein 3 )

HSP 1 Score: 312.4 bits (799), Expect = 8.5e-85
Identity = 190/458 (41.48%), Postives = 284/458 (62.01%), Query Frame = 0

Query: 141 SWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANL 200
           SWK L+ + GPGFL+SIA++DPGN E DLQ+GA   Y LLW+++ A+   L++Q L+A L
Sbjct: 52  SWKKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARL 111

Query: 201 GVVTGKHLAEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFS--IPVWCG 260
           GV TG+HLAE C+ EYP     +LWV+AE+A++  DI EVIG+A A+ +L +  +P+W G
Sbjct: 112 GVATGRHLAELCRDEYPTWARMVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAG 171

Query: 261 VLLTGLSTLLLLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQL 320
           V++T L   + L L+ YGIRKLE + A+L+ T+ + F    G AKP   E+L G+ VP+L
Sbjct: 172 VVITALDCFVFLFLENYGIRKLEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKL 231

Query: 321 KGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSVNGIKEACRFYMIESGFALMVA 380
             S     A+ ++G ++MPHN+FLHSALV SR++  R    ++EA  +Y IES  AL ++
Sbjct: 232 -SSRTIQKAVGVVGCIIMPHNVFLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFIS 291

Query: 381 FLINVSVISVSGAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSKLF--AIALLA 440
           FLIN+ V +V      A      D  +   L +N   +L     G     L+  AI LLA
Sbjct: 292 FLINLFVTTVF-----AKGFYNTDLANSIGL-VNAGQYLQEKYGGGVFPILYIWAIGLLA 351

Query: 441 SGQSSTITGTYAGQYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLII- 500
           +GQSSTITGTYAGQ++M GFL+ K++ W+R  +TRS AI+P++IVA++  SS A   ++ 
Sbjct: 352 AGQSSTITGTYAGQFIMGGFLNFKMKKWLRALITRSCAIIPTIIVALVFDSSEATLDVLN 411

Query: 501 -IASMILSFELPFALVPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRF 560
              +++ S ++PFAL+PLL   S +  MG      +   + W++  L++ IN Y L+  F
Sbjct: 412 EWLNVLQSIQIPFALIPLLCLVSKEQIMGSFKIGPLYKTIAWLVAALVIMINGYLLLEFF 471

Query: 561 IHVLLHNDLQLAAVVFIGILGFSGMALYLAGIAYLVFR 592
            +       +++ +V+ G +     A Y A I YL+ R
Sbjct: 472 SN-------EVSGIVYTGFVTLF-TASYGAFILYLIAR 494

BLAST of Moc08g10180 vs. TAIR 10
Match: AT1G47240.1 (NRAMP metal ion transporter 2 )

HSP 1 Score: 307.8 bits (787), Expect = 2.1e-83
Identity = 184/459 (40.09%), Postives = 280/459 (61.00%), Query Frame = 0

Query: 137 PDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSL 196
           P   SW+ L+ + GPGFL+SIA++DPGN E DLQ+GA   Y LLW+++ A+   L+IQ L
Sbjct: 60  PPPFSWRKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQML 119

Query: 197 AANLGVVTGKHLAEHCKAEYPRVQNFILWVLAEIAIVACDIPEVIGTAFALNMLFS--IP 256
           +A +GV TG+HLAE C+ EYP    ++LW +AE+A++  DI EVIG+A A+ +L    +P
Sbjct: 120 SARVGVATGRHLAELCRDEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLP 179

Query: 257 VWCGVLLTGLSTLLLLALQQYGIRKLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLF 316
           +W GV++T     L L L+ YG+RKLE + A+L+ T+ + F    G  KP   E++ G+ 
Sbjct: 180 LWAGVVITASDCFLFLFLENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGIL 239

Query: 317 VPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSVNGIKEACRFYMIESGFA 376
           +P+L  S     A+ ++G ++MPHN+FLHSALV SRKI P+  + ++EA  +Y+IES  A
Sbjct: 240 LPRL-SSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVA 299

Query: 377 LMVAFLINVSVISV-SGAVCNAPNLNKEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIA 436
           L ++F+IN+ V +V +         N    ++       K    L  +L  W      I 
Sbjct: 300 LFISFMINLFVTTVFAKGFYGTEKANNIGLVNAGQYLQEKFGGGLLPILYIW-----GIG 359

Query: 437 LLASGQSSTITGTYAGQYVMQGFLDLKLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKL 496
           LLA+GQSSTITGTYAGQ++M GFL+L+L+ W+R  +TRS AIVP++IVAI+  +S A   
Sbjct: 360 LLAAGQSSTITGTYAGQFIMGGFLNLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEASLD 419

Query: 497 II--IASMILSFELPFALVPLLKFTSSKTKMGPHVNSTVITVLTWIIGFLIMAINIYYLM 556
           ++    +++ S ++PFAL+PLL   S +  MG      ++  + W +  L+M IN Y L+
Sbjct: 420 VLNEWLNVLQSVQIPFALLPLLTLVSKEEIMGDFKIGPILQRIAWTVAALVMIINGYLLL 479

Query: 557 TRFIHVLLHNDLQLAAVVFIGILGFSGMALYLAGIAYLV 590
             F+        ++   +F G+        Y+A I YL+
Sbjct: 480 DFFVS-------EVDGFLF-GVTVCVWTTAYIAFIVYLI 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153441.18.6e-297100.00metal transporter Nramp6 [Momordica charantia][more]
XP_038905496.11.0e-28194.86metal transporter Nramp6-like [Benincasa hispida] >XP_038905497.1 metal transpor... [more]
TYK27020.11.3e-28192.63metal transporter Nramp6 [Cucumis melo var. makuwa][more]
KAA0037704.11.5e-27793.39metal transporter Nramp6 [Cucumis melo var. makuwa][more]
XP_023523752.11.7e-27691.74metal transporter Nramp6-like [Cucurbita pepo subsp. pepo] >XP_023523753.1 metal... [more]
Match NameE-valueIdentityDescription
Q9SAH88.8e-22877.06Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1[more]
Q9S9N81.8e-22578.39Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2[more]
Q653V61.8e-21772.98Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2... [more]
Q8H4H52.5e-16661.54Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2... [more]
Q0D7E44.2e-16163.72Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1DKN34.2e-297100.00metal transporter Nramp6 OS=Momordica charantia OX=3673 GN=LOC111020946 PE=3 SV=... [more]
A0A5D3DUP06.5e-28292.63Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7T2K97.4e-27893.39Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A0A0LD682.8e-27791.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G714870 PE=3 SV=1[more]
A0A6J1GBE73.1e-27691.56metal transporter Nramp6-like OS=Cucurbita moschata OX=3662 GN=LOC111452625 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G80830.16.3e-22977.06natural resistance-associated macrophage protein 1 [more]
AT1G15960.11.3e-22678.39NRAMP metal ion transporter 6 [more]
AT5G67330.11.6e-8641.65natural resistance associated macrophage protein 4 [more]
AT2G23150.18.5e-8541.48natural resistance-associated macrophage protein 3 [more]
AT1G47240.12.1e-8340.09NRAMP metal ion transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 465..482
score: 53.78
coord: 327..350
score: 63.0
coord: 277..298
score: 39.82
coord: 497..516
score: 51.0
coord: 437..456
score: 77.8
coord: 224..250
score: 56.74
coord: 252..271
score: 42.6
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 165..527
e-value: 3.7E-122
score: 407.7
IPR001046NRAMP familyTIGRFAMTIGR01197TIGR01197coord: 144..521
e-value: 2.0E-124
score: 413.8
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 106..636
IPR001046NRAMP familyHAMAPMF_00221NRAMPcoord: 130..553
score: 39.64185
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 611..641
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR11706:SF85METAL TRANSPORTER NRAMP1coord: 106..636

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g10180.1Moc08g10180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070574 cadmium ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0030001 metal ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015086 cadmium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity