Moc08g08730 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g08730
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chr8: 6502366 .. 6502998 (-)
RNA-Seq Expression	Moc08g08730
Synteny	Moc08g08730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAATTGTACGATTTCTCAGATTCAATTCACCCGAAAATCCTCTTGTTAATCTATAGCTGGCATGTGTATTTCTTTCTTGAAATTATCCTGGCCGTGGCGCAGTTTCTCGGCCGGCATCTCCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCCTATTTCTCCACTTCGCTTCAGGACTTTTGGGGGAGACGGTGGAATCTGACGGCGACGAACATTCTTCAGCCCACCGTTTACAGACCGACCGTACGCCTCGCCTCCCGCGTAGTCGGCCGGAGGTGGGCGCCGCTGCCGGCGGTGATGGCGACCTTTATGGTCTCGGCGATGATGCACGAACTGATTTTCTACTATTTGGGCCGAATGATGCCCAACTGGGAGATCACGTGGTTCTTCCTCATCCACGGATTTGCGTTGACGGTGGAGATTATGGTGAAGAAGATCCTACCGGAGAAGCTGCGGCTGCCGGCGGTGGTGTCGGGGCCGGCGACGGTGGCGTTCATCTTCTCCACCGCGATCTGGCTGTTCTTCCCGCAGTTCGTGAGGTTGAAGATAGACGAGAAGGCGTTTGAGGAGTACGCCGCGTTGGTCAGTTTCGTGAAGAACGGCGCTTTCTCATTGGTCGGTTGA

mRNA sequence

ATGGTGAAATTGTACGATTTCTCAGATTCAATTCACCCGAAAATCCTCTTGTTAATCTATAGCTGGCATGTGTATTTCTTTCTTGAAATTATCCTGGCCGTGGCGCAGTTTCTCGGCCGGCATCTCCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCCTATTTCTCCACTTCGCTTCAGGACTTTTGGGGGAGACGGTGGAATCTGACGGCGACGAACATTCTTCAGCCCACCGTTTACAGACCGACCGTACGCCTCGCCTCCCGCGTAGTCGGCCGGAGGTGGGCGCCGCTGCCGGCGGTGATGGCGACCTTTATGGTCTCGGCGATGATGCACGAACTGATTTTCTACTATTTGGGCCGAATGATGCCCAACTGGGAGATCACGTGGTTCTTCCTCATCCACGGATTTGCGTTGACGGTGGAGATTATGGTGAAGAAGATCCTACCGGAGAAGCTGCGGCTGCCGGCGGTGGTGTCGGGGCCGGCGACGGTGGCGTTCATCTTCTCCACCGCGATCTGGCTGTTCTTCCCGCAGTTCGTGAGGTTGAAGATAGACGAGAAGGCGTTTGAGGAGTACGCCGCGTTGGTCAGTTTCGTGAAGAACGGCGCTTTCTCATTGGTCGGTTGA

Coding sequence (CDS)

ATGGTGAAATTGTACGATTTCTCAGATTCAATTCACCCGAAAATCCTCTTGTTAATCTATAGCTGGCATGTGTATTTCTTTCTTGAAATTATCCTGGCCGTGGCGCAGTTTCTCGGCCGGCATCTCCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCCTATTTCTCCACTTCGCTTCAGGACTTTTGGGGGAGACGGTGGAATCTGACGGCGACGAACATTCTTCAGCCCACCGTTTACAGACCGACCGTACGCCTCGCCTCCCGCGTAGTCGGCCGGAGGTGGGCGCCGCTGCCGGCGGTGATGGCGACCTTTATGGTCTCGGCGATGATGCACGAACTGATTTTCTACTATTTGGGCCGAATGATGCCCAACTGGGAGATCACGTGGTTCTTCCTCATCCACGGATTTGCGTTGACGGTGGAGATTATGGTGAAGAAGATCCTACCGGAGAAGCTGCGGCTGCCGGCGGTGGTGTCGGGGCCGGCGACGGTGGCGTTCATCTTCTCCACCGCGATCTGGCTGTTCTTCCCGCAGTTCGTGAGGTTGAAGATAGACGAGAAGGCGTTTGAGGAGTACGCCGCGTTGGTCAGTTTCGTGAAGAACGGCGCTTTCTCATTGGTCGGTTGA

Protein sequence

MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSLQDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYLGRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQFVRLKIDEKAFEEYAALVSFVKNGAFSLVG

Homology

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BLAST of Moc08g08730 vs. NCBI nr
Match: XP_022152587.1 (acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia])

HSP 1 Score: 421.0 bits (1081), Expect = 6.0e-114
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL
Sbjct: 139 MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 198

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL
Sbjct: 199 QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 258

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ
Sbjct: 259 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 318

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLVG 211
           FVRLKIDEKAFEEYAALVSFVKNGAFSLVG
Sbjct: 319 FVRLKIDEKAFEEYAALVSFVKNGAFSLVG 348

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BLAST of Moc08g08730 vs. NCBI nr
Match: KAG6581559.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 358.6 bits (919), Expect = 3.7e-95
Identity = 170/209 (81.34%), Postives = 192/209 (91.87%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWHVYFFLEIILA+ QF GR LLGIELEPQF+DPYFSTSL
Sbjct: 126 MVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFGRTLLGIELEPQFHDPYFSTSL 185

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPT+Y+PTV++A++VVGR+WAPLP VMATF+VSA+MHELIFYYL
Sbjct: 186 QDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPLPGVMATFLVSAIMHELIFYYL 245

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE+TWFF+IHGFALTVEI+VKK+LPEK RLPA VS P T+ FIFSTAIWLFFPQ
Sbjct: 246 GRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQ 305

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLV 210
           F+RLKID KAFEEYAALVSF+K  AF LV
Sbjct: 306 FMRLKIDVKAFEEYAALVSFIKKLAFPLV 334

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BLAST of Moc08g08730 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 358.6 bits (919), Expect = 3.7e-95
Identity = 170/209 (81.34%), Postives = 192/209 (91.87%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWHVYFFLEIILA+ QF GR LLGIELEPQF+DPYFSTSL
Sbjct: 142 MVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFGRTLLGIELEPQFHDPYFSTSL 201

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPT+Y+PTV++A++VVGR+WAPLP VMATF+VSA+MHELIFYYL
Sbjct: 202 QDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPLPGVMATFLVSAIMHELIFYYL 261

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE+TWFF+IHGFALTVEI+VKK+LPEK RLPA VS P T+ FIFSTAIWLFFPQ
Sbjct: 262 GRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQ 321

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLV 210
           F+RLKID KAFEEYAALVSF+K  AF LV
Sbjct: 322 FMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

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BLAST of Moc08g08730 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 358.6 bits (919), Expect = 3.7e-95
Identity = 170/209 (81.34%), Postives = 192/209 (91.87%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWHVYFFLEIILA+ QF GR LLGIELEPQF+DPYFSTSL
Sbjct: 142 MVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFGRTLLGIELEPQFHDPYFSTSL 201

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPT+Y+PTV++A++VVGR+WAPLP VMATF+VSA+MHELIFYYL
Sbjct: 202 QDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPLPGVMATFLVSAIMHELIFYYL 261

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE+TWFF+IHGFALTVEI+VKK+LPEK RLPA VS P T+ FIFSTAIWLFFPQ
Sbjct: 262 GRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQ 321

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLV 210
           F+RLKID KAFEEYAALVSF+K  AF LV
Sbjct: 322 FMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

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BLAST of Moc08g08730 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 358.2 bits (918), Expect = 4.8e-95
Identity = 171/209 (81.82%), Postives = 192/209 (91.87%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWHVYFFLEIILA+ QF GR LLGIELEPQF+DPYFSTSL
Sbjct: 142 MVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFGRTLLGIELEPQFHDPYFSTSL 201

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPTVY+PTV++A++VVGR+WAPLP VMATF+VSA+MHELIFYYL
Sbjct: 202 QDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPLPGVMATFLVSAIMHELIFYYL 261

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE+TWFF+IHGFALTVEI+VKK+LPEK RLPA VS P T+ FIFSTAIWLFFPQ
Sbjct: 262 GRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQ 321

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLV 210
           F+RLKID KAFEEYAALVSF+K  AF LV
Sbjct: 322 FMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

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BLAST of Moc08g08730 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.6e-53
Identity = 101/200 (50.50%), Postives = 142/200 (71.00%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++K Y++S  +  K++L +Y+ H+YF LEIILA      R +  +ELEPQFN PY +TSL
Sbjct: 134 IIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFNKPYLATSL 193

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL  T IL+PTVY P+++L S V+G  ++ + A   TF+VS +MHELIF+Y+
Sbjct: 194 QDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVSGIMHELIFFYM 253

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GR+ P+W++ WFFLI+GF  TVEI +KK +  + R P  +S   T+ F+  TA+WLF P+
Sbjct: 254 GRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVMVTALWLFLPE 313

Query: 181 FVRLKIDEKAFEEYAALVSF 201
           F R  I EKA +EYAA+ +F
Sbjct: 314 FNRCNIVEKALDEYAAIGAF 332

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BLAST of Moc08g08730 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 2.5e-46
Identity = 91/210 (43.33%), Postives = 132/210 (62.86%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++K+Y+F   +   ++ ++Y +H Y   EI LA +  + R  LG++L PQFN+PY +TSL
Sbjct: 142 ILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARATLGLDLYPQFNEPYLATSL 201

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL  ++IL  T Y+P  R+ SR V  RW    A++  F VS +MHE+ F+YL
Sbjct: 202 QDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAMLVAFTVSGLMHEVFFFYL 261

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
            R  P+WE+T FF++HG    VE++VKK +  K+RL   VSG  TV F+  T  WLF PQ
Sbjct: 262 TRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSGALTVGFVMVTGGWLFLPQ 321

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLVG 211
            VR  +D K  +EY  + ++ +     L+G
Sbjct: 322 LVRHGVDLKTIDEYPVMFNYTQKKLMGLLG 351

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BLAST of Moc08g08730 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.4e-41
Identity = 91/204 (44.61%), Postives = 125/204 (61.27%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++ LY +  ++ P ILL +Y  H+Y  +EIIL   + +    LG +LEPQ N PY +TSL
Sbjct: 129 LLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSNKPYLATSL 188

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL    IL+P VY P  R++ R +   WA  P ++A F+VS ++HEL+F+YL
Sbjct: 189 QDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLVHELLFFYL 248

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKK--ILPEKLRLPAVVSGPATVAFIFSTAIWLFF 180
            R MP  E+T FF++HG    VE+ VKK   + ++ RL   VS   TV F+F T  WLF 
Sbjct: 249 IREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLTVGFVFVTGGWLFT 308

Query: 181 PQFVRLKIDEKAFEEYAALVSFVK 203
           PQ  R  + E+   E   LV F+K
Sbjct: 309 PQLKRSGVMERFTSEAVLLVEFIK 332

[Close]

BLAST of Moc08g08730 vs. ExPASy Swiss-Prot
Match: Q9FJ77 (Probable long-chain-alcohol O-fatty-acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=AT6 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.9e-38
Identity = 84/212 (39.62%), Postives = 121/212 (57.08%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           +++ Y +   +    LL +Y+ H+Y  LEI L + +FL    LG +LEPQFN+PY +TSL
Sbjct: 132 LLQAYHYKQFLSTNFLLGLYALHIYLELEISLTLIKFLVSITLGCDLEPQFNEPYLATSL 191

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
            DFWG RWNL  + IL   VY P  +   R     W    A+ ATF+VS + HE++++YL
Sbjct: 192 HDFWGHRWNLMVSKILWLAVYNPIRQW--RAKSSEWDRFFAIFATFLVSGVAHEILYFYL 251

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPA--VVSGPATVAFIFSTAIWLFF 180
            R  P WE+TWFF++HGF +  E+ +K+      R P    VS   TV F+F T +WLF 
Sbjct: 252 TREKPTWEVTWFFVLHGFCMAAEVALKRKTKLVQRWPVNPAVSRLLTVGFVFVTGVWLFS 311

Query: 181 PQFVRLKIDEKAFEEYAALVSFVKNGAFSLVG 211
           PQ +R  + E+   E   L+ F     + L+G
Sbjct: 312 PQPIRHGLMERFINEDLFLIDFFNRKLYILLG 341

[Close]

BLAST of Moc08g08730 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.6e-37
Identity = 85/209 (40.67%), Postives = 122/209 (58.37%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++ +Y++   + P +LL++YS H+Y   EI+LA  + L    L  +LEP FN+PY STSL
Sbjct: 129 LLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNEPYLSTSL 188

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL    IL+P VY P  ++A R +    A    V A+F+VS ++HELIF+Y 
Sbjct: 189 QDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVHELIFFYF 248

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKI-LPEKLRLPAVVSGPATVAFIFSTAIWLFFP 180
            R  P  E+T FF++HG     E   K+  L  + ++  +VS   TV F+  T  WLFFP
Sbjct: 249 TRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVGFVVMTGGWLFFP 308

Query: 181 QFVRLKIDEKAFEEYAALVSFVKNGAFSL 209
              R  + E+  +E    + FVK+  F L
Sbjct: 309 HLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Moc08g08730 vs. ExPASy TrEMBL
Match: A0A6J1DGN9 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020279 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 2.9e-114
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL
Sbjct: 139 MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 198

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL
Sbjct: 199 QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 258

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ
Sbjct: 259 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 318

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLVG 211
           FVRLKIDEKAFEEYAALVSFVKNGAFSLVG
Sbjct: 319 FVRLKIDEKAFEEYAALVSFVKNGAFSLVG 348

[Close]

BLAST of Moc08g08730 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.8e-95
Identity = 170/209 (81.34%), Postives = 192/209 (91.87%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWHVYFFLEIILA+ QF GR LLGIELEPQF+DPYFSTSL
Sbjct: 142 MVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFGRTLLGIELEPQFHDPYFSTSL 201

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPT+Y+PTV++A++VVGR+WAPLP VMATF+VSA+MHELIFYYL
Sbjct: 202 QDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPLPGVMATFLVSAIMHELIFYYL 261

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE+TWFF+IHGFALTVEI+VKK+LPEK RLPA VS P T+ FIFSTAIWLFFPQ
Sbjct: 262 GRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQ 321

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLV 210
           F+RLKID KAFEEYAALVSF+K  AF LV
Sbjct: 322 FMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of Moc08g08730 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.3e-95
Identity = 171/209 (81.82%), Postives = 192/209 (91.87%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWHVYFFLEIILA+ QF GR LLGIELEPQF+DPYFSTSL
Sbjct: 142 MVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFGRTLLGIELEPQFHDPYFSTSL 201

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPTVY+PTV++A++VVGR+WAPLP VMATF+VSA+MHELIFYYL
Sbjct: 202 QDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPLPGVMATFLVSAIMHELIFYYL 261

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE+TWFF+IHGFALTVEI+VKK+LPEK RLPA VS P T+ FIFSTAIWLFFPQ
Sbjct: 262 GRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQ 321

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLV 210
           F+RLKID KAFEEYAALVSF+K  AF LV
Sbjct: 322 FMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of Moc08g08730 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 168/210 (80.00%), Postives = 192/210 (91.43%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWH+YF LEIILA+ QF GR++LGIELEPQF DPYFSTSL
Sbjct: 140 MVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRNVLGIELEPQFQDPYFSTSL 199

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPTVY+P+V++A++V+GR+WAPLPAVMATF+VSAMMHELIFYY+
Sbjct: 200 QDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPAVMATFLVSAMMHELIFYYM 259

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE TWFF+IHG ALTVEIMVKK+LPEK RLP  VS P T+AFIF TAIWLFFPQ
Sbjct: 260 GRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPAVSVPVTLAFIFPTAIWLFFPQ 319

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLVG 211
           FVRLK+D +AFEEYAALV+FVKN AFSLVG
Sbjct: 320 FVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of Moc08g08730 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.4e-94
Identity = 167/210 (79.52%), Postives = 192/210 (91.43%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           MVKLYD+S+ IHPK++L IYSWH+YF LEIILA+ QF GR++LGIELEPQF DPYFSTSL
Sbjct: 140 MVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRNVLGIELEPQFQDPYFSTSL 199

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL ATNILQPTVY+P+V++A++V+GR+WAPLPAVMATF+VSAMMHELIF+Y+
Sbjct: 200 QDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPAVMATFLVSAMMHELIFFYM 259

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GRM PNWE TWFF+IHG ALTVEIMVKK+LPEK RLP  VS P T+AFIF TAIWLFFPQ
Sbjct: 260 GRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPAVSVPVTLAFIFPTAIWLFFPQ 319

Query: 181 FVRLKIDEKAFEEYAALVSFVKNGAFSLVG 211
           FVRLK+D +AFEEYAALV+FVKN AFSLVG
Sbjct: 320 FVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of Moc08g08730 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 210.7 bits (535), Expect = 1.1e-54
Identity = 101/200 (50.50%), Postives = 142/200 (71.00%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++K Y++S  +  K++L +Y+ H+YF LEIILA      R +  +ELEPQFN PY +TSL
Sbjct: 134 IIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFNKPYLATSL 193

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL  T IL+PTVY P+++L S V+G  ++ + A   TF+VS +MHELIF+Y+
Sbjct: 194 QDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVSGIMHELIFFYM 253

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPAVVSGPATVAFIFSTAIWLFFPQ 180
           GR+ P+W++ WFFLI+GF  TVEI +KK +  + R P  +S   T+ F+  TA+WLF P+
Sbjct: 254 GRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVMVTALWLFLPE 313

Query: 181 FVRLKIDEKAFEEYAALVSF 201
           F R  I EKA +EYAA+ +F
Sbjct: 314 FNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of Moc08g08730 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 170.2 bits (430), Expect = 1.7e-42
Identity = 91/204 (44.61%), Postives = 125/204 (61.27%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++ LY +  ++ P ILL +Y  H+Y  +EIIL   + +    LG +LEPQ N PY +TSL
Sbjct: 129 LLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSNKPYLATSL 188

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL    IL+P VY P  R++ R +   WA  P ++A F+VS ++HEL+F+YL
Sbjct: 189 QDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLVHELLFFYL 248

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKK--ILPEKLRLPAVVSGPATVAFIFSTAIWLFF 180
            R MP  E+T FF++HG    VE+ VKK   + ++ RL   VS   TV F+F T  WLF 
Sbjct: 249 IREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLTVGFVFVTGGWLFT 308

Query: 181 PQFVRLKIDEKAFEEYAALVSFVK 203
           PQ  R  + E+   E   LV F+K
Sbjct: 309 PQLKRSGVMERFTSEAVLLVEFIK 332

[Close]

BLAST of Moc08g08730 vs. TAIR 10
Match: AT5G55330.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.4e-39
Identity = 84/212 (39.62%), Postives = 121/212 (57.08%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           +++ Y +   +    LL +Y+ H+Y  LEI L + +FL    LG +LEPQFN+PY +TSL
Sbjct: 132 LLQAYHYKQFLSTNFLLGLYALHIYLELEISLTLIKFLVSITLGCDLEPQFNEPYLATSL 191

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
            DFWG RWNL  + IL   VY P  +   R     W    A+ ATF+VS + HE++++YL
Sbjct: 192 HDFWGHRWNLMVSKILWLAVYNPIRQW--RAKSSEWDRFFAIFATFLVSGVAHEILYFYL 251

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKILPEKLRLPA--VVSGPATVAFIFSTAIWLFF 180
            R  P WE+TWFF++HGF +  E+ +K+      R P    VS   TV F+F T +WLF 
Sbjct: 252 TREKPTWEVTWFFVLHGFCMAAEVALKRKTKLVQRWPVNPAVSRLLTVGFVFVTGVWLFS 311

Query: 181 PQFVRLKIDEKAFEEYAALVSFVKNGAFSLVG 211
           PQ +R  + E+   E   L+ F     + L+G
Sbjct: 312 PQPIRHGLMERFINEDLFLIDFFNRKLYILLG 341

[Close]

BLAST of Moc08g08730 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-38
Identity = 85/209 (40.67%), Postives = 122/209 (58.37%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++ +Y++   + P +LL++YS H+Y   EI+LA  + L    L  +LEP FN+PY STSL
Sbjct: 129 LLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNEPYLSTSL 188

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL    IL+P VY P  ++A R +    A    V A+F+VS ++HELIF+Y 
Sbjct: 189 QDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVHELIFFYF 248

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKKI-LPEKLRLPAVVSGPATVAFIFSTAIWLFFP 180
            R  P  E+T FF++HG     E   K+  L  + ++  +VS   TV F+  T  WLFFP
Sbjct: 249 TRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVGFVVMTGGWLFFP 308

Query: 181 QFVRLKIDEKAFEEYAALVSFVKNGAFSL 209
              R  + E+  +E    + FVK+  F L
Sbjct: 309 HLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Moc08g08730 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.0e-38
Identity = 85/205 (41.46%), Postives = 121/205 (59.02%), Query Frame = 0


Query: 1   MVKLYDFSDSIHPKILLLIYSWHVYFFLEIILAVAQFLGRHLLGIELEPQFNDPYFSTSL 60
           ++++Y++   + P +LL+  S H++  LEI+  + + L    LG +LEPQ N+PY +TSL
Sbjct: 130 LLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDLEPQSNEPYLATSL 189

Query: 61  QDFWGRRWNLTATNILQPTVYRPTVRLASRVVGRRWAPLPAVMATFMVSAMMHELIFYYL 120
           QDFWGRRWNL    IL+P VY P  R+A R V    A    V A F+VS  +HE++F+YL
Sbjct: 190 QDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFLVSGAVHEMLFFYL 249

Query: 121 GRMMPNWEITWFFLIHGFALTVEIMVKK--ILPEKLRLPAVVSGPATVAFIFSTAIWLFF 180
            R +P  E+TWFFL+HG     E+ VKK   +    R+   VS   TV F+  T+ W FF
Sbjct: 250 TREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLTVGFVVVTSGWFFF 309

Query: 181 PQFVRLKIDEKAFEEYAALVSFVKN 204
           P  +R  I E+   E    + FVK+
Sbjct: 310 P-LIRSGIIERLASEALMCIDFVKH 333

The following BLAST results are available for this feature:

BLAST of Moc08g08730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152587.1	6.0e-114	100.00	acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia]	[more]
KAG6581559.1	3.7e-95	81.34	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_023527814.1	3.7e-95	81.34	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022934183.1	3.7e-95	81.34	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_022982638.1	4.8e-95	81.82	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]

BLAST of Moc08g08730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.6e-53	50.50	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	2.5e-46	43.33	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ76	2.4e-41	44.61	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ77	1.9e-38	39.62	Probable long-chain-alcohol O-fatty-acyltransferase 6 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	1.6e-37	40.67	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]

BLAST of Moc08g08730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGN9	2.9e-114	100.00	acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1F6Y6	1.8e-95	81.34	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J5C4	2.3e-95	81.82	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBE5	1.5e-94	80.00	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KAA2	4.4e-94	79.52	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Moc08g08730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	1.1e-54	50.50	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	1.7e-42	44.61	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55330.1	1.4e-39	39.62	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.2e-38	40.67	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	2.0e-38	41.46	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 48..134 e-value: 1.5E-15 score: 57.2
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 1..203
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 1..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g08730.1	Moc08g08730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity