Moc08g08690 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g08690
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	structure-specific endonuclease subunit SLX1
Location	chr8: 6478925 .. 6482684 (+)
RNA-Seq Expression	Moc08g08690
Synteny	Moc08g08690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACAAATTGTAGTTGAGCATGAATCAACTGTCAATCAAACGGCCAAACGGCAATTTCCGAACCGGCGCCAAAGAAGAGGAACCAGGGAGCGAATGAGACTCCTCTCTCACACATTTCGTTGCCATAAATCCCCAATTTCAAATCCAAAACCCTCGTCTTCTTCTTCAACCAAGGATCCATCTGCTGCGACTTTGAGCTTCGAATCGCAGCTGAAGCCGAAGCCATGGTGTGTGTATCTCATCATCTCCTCCAATTCCCCCTTCAAAACCTATGTTGGGGTCACCCTCAACTTCTCTCGCCGGTAAGTCCAATTTATGCTGTTGGGTTCGCACAAATTTTTTATGAATCGATTTTCGATTTTATCTGGGTCATGTTCGAAGTGATTTTACGAGCCATTTGGGTTCAACTTTGGTGAAACAGTGGAATTTTTGTAAGAAACACGTCCTGATTATGTCGCTTGTTTGGAGATAACCTCGTATCGAGTCAATTTGGTGCAAGAGGATGAGGTTTGTCGTGAAAAATAAATCTAGGGTTTGGTTTTTTATTGACAGTTTCCAAGTTGAACTGCGTTTTTTTTTTTTAATGTTTAGCGAGAGAATTTGAAGTAGGGAGTGTTTTGGAGTCATCTCCGAACTGTTGTGGCATTTTAGAAGTTATGGAAGGATTCTGGAGGGAAGACCAGTATTCTTCAAGTTTTCATAAGAGATATACTTCAAATGTTTTTCTGGAACCCAATCGTCTAGCCAATTCCTTGGCCAAAAAAAATATTTGCAACTCCTGAATCCTGATTTTTACAAATACTAGTTGAACACTTGGGATCTCATTTCTGTTCTTTGACGTGCACGTTGTGTCCTCGTTGATTTACATGTTATTTGTGCATAAGGCGTATTTCAATATTTCTTATTGTCATAAAAGTTGTATTCTTGTACAGTTTGAAACAGCATAATGGTGAAATAAAAGGCGGTGCAAAAGCAACTCGAGCAGGACGACCCTGGGTTTGTGCGTGCATGATTCATGGTTTTAAAGACCAAAGTCAAGGTATGCATTTAAAATAGAACTTCTAGAATCATGCACCTGTTGAGTTCCATATTTCAAGGGCCTTTCTTTGGCTATTTGTGATAGTAGTAATCTAGAAGCAATGTTTTTGGTCTGTTGCTTCCTTATGGAGGCGTTATGTTTCTTGCATGAGATATTCTGAAATATTTACGGCTACAAATTTTCTTTTTTGAAATTTTCTTCTTCATTACCGTACTGTACATGTTTCGGTAATTGATCAGCTACAAAGTTGCTGCATATTGTGATTCAGCTTTGTCGTAGTTGCTTTGTTGTGGCGACGATCTTGCTATATTAGCTGACACCAGTCAGATGTATTTCAAATTTTTTCTGGCATTGTGAGAACATTTCCCCTGAGACACTAGCTTTATCCATTGCTCCTCTTGGAGAAGAAGCATTATACTTGGTTCGAACTTTTAAAATACTATATAAATGCTGACAATTTCCTTTTTTCATAAGATACAAAATTAGTTTACTCAAGGCCAAAGAAGATACCCTTGATGAGTGGATCCTTAAGTACTGGCCTTCAGAATTTGAGCCTTTTTCTGTAAAGAGTTAGTTTAGATGTTCCCTTTATTGGGTTGTTTTTCTTATGCCCTTATATATTCTTTCATCTTCTCTAGTTGGTTTTCCGATAAAAAAATATCAATAAGTACTATAGAACTCGTCCTAGAAGTACCATGAAGGATAATATGCATAATGGTACTTGCTCTTGAAGCAGTGATGATGGGGCCAAAGAGAGACAAAAGGTAAATGGGTATGCTATGCTCGAGATTATATACTCTAAGAGGAAGGGAGGGGGATAGGAAAGTAACTTTATAGCATAATACCCTATACTTGTAATTCATAAATTAAAGGCTGAAGTGTTTCTTTTTATAAGGTACAAAGATTAGTTCATTCAAGGTAGATGACGGTACTGTGGCTAGTAGATTGGCAAGTACTGTAACTCCCATGCCAGAATAGCCTAACAATGCATTCTAGTTTTTGGAACTATCTAGTCATTTCAGTTACGATGAACCAACGAGACCAGAAATTGGTGACACATATTGTAGTGTCGTACAAAGTAGTTGGAATTTGTACTCAATTATTAAGTGGTAGGGGGCTCACTGGTTGAAGAGTTGTAACTACACCTAACATGTGAAATGGGTGCATAAAGATGTTACGTTCGACTAGGAATAGAACCTCGAAGTTGATAGTACTTGAGATTCCTTTGAGGTAGATTGTCATTAAAGTTTTGTTAACAAATTGGATGAATAAAGAAAGTTAGTAGAAGTAGTCGTTGCATCAATTCCAAGGGGCATACCCTGTTAAGAACAAGTTCATGAGATCGTCCCATGATAAAGGCTACACCGATTAAGAAGTGTAGTGCAGTTAGCTCATAAGGACCACCCTTTTATAACTAGGAAGTAACTTCCAATATCGGGTAAAAGTACATCCTAGAGCTGGAGCAGGGATAATAGCACCAAAATTAAATTGTTTCCTTAAAGTAGAGATCTAGTGACAAATTTTTGAATATTATCAATAATATCGATTATCTACCTTAGGAGCAGCAATGAAAGCAATAATTAATAATTAAAAAAAAATTAATATAGAAGTAGTGGTCAGGGCAATGACAGTTTTAGGGGCCCCATAAGCCATTTCTGTAGTACATTAGCCATGATATTAATTGTGATTCCGATGGCTGATACAACTTTATACATTCGAACCAAACCTTTTCATTATTTCATACGCATAATGCAACTGTCATTCCTCTTTCATATCAAATTGGTAACAATCAAATCTCACATCATATGCATTAAGGGGGCCTTCCATCTTTATTGTAATATTAATTTGTTTGATTTTTTTGTTTTGTGTGTGTGTGTGTGTATGAAGCCATTTGGAACTGTCGGCACCCTTAAAAGATCAAATTCTTTCTTTGGGAGTTTTGCCAAAAGGCTATAAATACTTATGACAGCTCCAAGATAGGATGTCTTTCATGGTTTTATCTCCACAATGGTGCTCTATTTGCAAGAGAAGCCACAAGTCACAACAACACCTTTTTCTCTCATGTGAATATGCAGCAATCTTTTGGATTTTTCTTCTGTCCATACACGCCCCCCCAAGCTACAGCTTTTTGGTTCACTATATATACATATAGTATCCCATTCTTCAATCACGACTTTTGGTAACCTTAAAATCATCGAGTCTCTTGTGTATTGATGATTGATTGTAGTGAAGATATTGTTTCAATTTATGTTGTTGCTATGAAAATCCTTCAAATTCATGCTCCTTGATCATTCCCCAGAAATTTCACTTGTTTGAAATTTTTACACTACAGTTTTAACTCATTGGTCGGAGACATCGATAATAAAGAGCAATCTAACTCAACGGGGTAGTCGTATTAAAAACCCTTTGGGCCAGACCACCAACCCAATTATCACGTGTCTCTGAGTTTTTCAACTGGTAAATGTACCGTAAACTCAGCCCCACACCATAACTGATGCCTGGTTTTTGTAATCTTTGGATGCAGCTTGTGAATTTGAATCAAAATGGAAAGAAGTTTCAAGGAAAATTGCCCATAAGAGAAAGAAGGATGATGAAGAAGAGCAGGCAGATGAACAGAAATTAAGATTGCTGAAGAACAGGCAAAGAGCTCTAGAAAAAGTGAAGTTTATGTTTGATTGCAGCCGATTTGAGTTTGATGGGCAATTGGATCCTTTCTGA

mRNA sequence

ATGAAACAAATTGTAGTTGAGCATGAATCAACTGTCAATCAAACGGCCAAACGGCAATTTCCGAACCGGCGCCAAAGAAGAGGAACCAGGGAGCGAATGAGACTCCTCTCTCACACATTTCGTTGCCATAAATCCCCAATTTCAAATCCAAAACCCTCGTCTTCTTCTTCAACCAAGGATCCATCTGCTGCGACTTTGAGCTTCGAATCGCAGCTGAAGCCGAAGCCATGGTGTGTGTATCTCATCATCTCCTCCAATTCCCCCTTCAAAACCTATGTTGGGGTCACCCTCAACTTCTCTCGCCGTTTGAAACAGCATAATGGTGAAATAAAAGGCGGTGCAAAAGCAACTCGAGCAGGACGACCCTGGGTTTGTGCGTGCATGATTCATGGTTTTAAAGACCAAAGTCAAGCTTGTGAATTTGAATCAAAATGGAAAGAAGTTTCAAGGAAAATTGCCCATAAGAGAAAGAAGGATGATGAAGAAGAGCAGGCAGATGAACAGAAATTAAGATTGCTGAAGAACAGGCAAAGAGCTCTAGAAAAAGTGAAGTTTATGTTTGATTGCAGCCGATTTGAGTTTGATGGGCAATTGGATCCTTTCTGA

Coding sequence (CDS)

ATGAAACAAATTGTAGTTGAGCATGAATCAACTGTCAATCAAACGGCCAAACGGCAATTTCCGAACCGGCGCCAAAGAAGAGGAACCAGGGAGCGAATGAGACTCCTCTCTCACACATTTCGTTGCCATAAATCCCCAATTTCAAATCCAAAACCCTCGTCTTCTTCTTCAACCAAGGATCCATCTGCTGCGACTTTGAGCTTCGAATCGCAGCTGAAGCCGAAGCCATGGTGTGTGTATCTCATCATCTCCTCCAATTCCCCCTTCAAAACCTATGTTGGGGTCACCCTCAACTTCTCTCGCCGTTTGAAACAGCATAATGGTGAAATAAAAGGCGGTGCAAAAGCAACTCGAGCAGGACGACCCTGGGTTTGTGCGTGCATGATTCATGGTTTTAAAGACCAAAGTCAAGCTTGTGAATTTGAATCAAAATGGAAAGAAGTTTCAAGGAAAATTGCCCATAAGAGAAAGAAGGATGATGAAGAAGAGCAGGCAGATGAACAGAAATTAAGATTGCTGAAGAACAGGCAAAGAGCTCTAGAAAAAGTGAAGTTTATGTTTGATTGCAGCCGATTTGAGTTTGATGGGCAATTGGATCCTTTCTGA

Protein sequence

MKQIVVEHESTVNQTAKRQFPNRRQRRGTRERMRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF

Homology

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BLAST of Moc08g08690 vs. NCBI nr
Match: XP_022152567.1 (structure-specific endonuclease subunit SLX1 [Momordica charantia])

HSP 1 Score: 399.8 bits (1026), Expect = 1.4e-107
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MKQIVVEHESTVNQTAKRQFPNRRQRRGTRERMRLLSHTFRCHKSPISNPKPSSSSSTKD 60
           MKQIVVEHESTVNQTAKRQFPNRRQRRGTRERMRLLSHTFRCHKSPISNPKPSSSSSTKD
Sbjct: 1   MKQIVVEHESTVNQTAKRQFPNRRQRRGTRERMRLLSHTFRCHKSPISNPKPSSSSSTKD 60

Query: 61  PSAATLSFESQLKPKPWCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG 120
           PSAATLSFESQLKPKPWCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG
Sbjct: 61  PSAATLSFESQLKPKPWCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG 120

Query: 121 RPWVCACMIHGFKDQSQACEFESKWKEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRAL 180
           RPWVCACMIHGFKDQSQACEFESKWKEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRAL
Sbjct: 121 RPWVCACMIHGFKDQSQACEFESKWKEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRAL 180

Query: 181 EKVKFMFDCSRFEFDGQLDPF 202
           EKVKFMFDCSRFEFDGQLDPF
Sbjct: 181 EKVKFMFDCSRFEFDGQLDPF 201

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BLAST of Moc08g08690 vs. NCBI nr
Match: XP_004151512.1 (structure-specific endonuclease subunit SLX1 [Cucumis sativus] >KGN58557.1 hypothetical protein Csa_001754 [Cucumis sativus])

HSP 1 Score: 245.0 bits (624), Expect = 5.6e-61
Identity = 128/173 (73.99%), Postives = 147/173 (84.97%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNP--KPSSSSSTKDPSAATLSFESQLKP--KPWCVYLIISSNSP 92
           MRLLSHTFRC K PISNP    SSSSS+KDP   TL  +S+ KP  K WCVYLIISSNSP
Sbjct: 1   MRLLSHTFRCVKPPISNPPLSKSSSSSSKDP---TLIVKSEPKPKLKQWCVYLIISSNSP 60

Query: 93  FKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEV 152
            KTYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWK+V
Sbjct: 61  IKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACTIHGFKDQSQACEFESKWKKV 120

Query: 153 SRKIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 202
           SRKI++++K++D  +  D+Q LRLLK+R+RAL KVK +FDCS+FEFD +LDPF
Sbjct: 121 SRKISYEKKEEDVGKLLDDQTLRLLKHRERALGKVKCLFDCSQFEFDWKLDPF 170

[Close]

BLAST of Moc08g08690 vs. NCBI nr
Match: XP_008461838.1 (PREDICTED: structure-specific endonuclease subunit slx1 [Cucumis melo] >XP_008461839.1 PREDICTED: structure-specific endonuclease subunit slx1 [Cucumis melo])

HSP 1 Score: 245.0 bits (624), Expect = 5.6e-61
Identity = 127/171 (74.27%), Postives = 142/171 (83.04%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 92
           MRLLS TFRC K PISNP+   SSSSSTK P+      E   KPK WCVYLIISSNSP K
Sbjct: 1   MRLLSQTFRCQKPPISNPRLPKSSSSSTKGPTLIVKLEEP--KPKQWCVYLIISSNSPIK 60

Query: 93  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 152
           TYVGVTL+F RRLKQHNGEIKGGAKAT+AGRPW+CAC IHGFKDQSQACEFESKWK+VSR
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATQAGRPWICACTIHGFKDQSQACEFESKWKKVSR 120

Query: 153 KIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 202
           KI++++K+DD  +Q D Q LRLLK+RQRAL KVK +FDCS FEFD +LDPF
Sbjct: 121 KISYEKKEDDVGKQLDNQTLRLLKHRQRALGKVKCLFDCSHFEFDWKLDPF 169

[Close]

BLAST of Moc08g08690 vs. NCBI nr
Match: XP_038903984.1 (structure-specific endonuclease subunit slx1 isoform X1 [Benincasa hispida])

HSP 1 Score: 238.0 bits (606), Expect = 6.9e-59
Identity = 125/171 (73.10%), Postives = 141/171 (82.46%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 92
           MRLLS TFR  K   SN +   SSSSSTKDP    L  +S+ KPK WCVYLIISSNSP K
Sbjct: 1   MRLLSQTFRTQKPSNSNSQLSKSSSSSTKDP---ILIVKSEPKPKQWCVYLIISSNSPIK 60

Query: 93  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 152
           TYVGVTL+F RRLKQHNGEIKGGAKATR GRPW+CAC IHGFKDQ QACEFESKWKEVSR
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRTGRPWLCACTIHGFKDQGQACEFESKWKEVSR 120

Query: 153 KIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 202
           KI++K+K+DD  +Q D+Q LRLLK+RQRAL KVK +FDCS+FEF+ +LDPF
Sbjct: 121 KISYKKKEDDMGKQVDDQTLRLLKHRQRALGKVKCLFDCSQFEFNWKLDPF 168

[Close]

BLAST of Moc08g08690 vs. NCBI nr
Match: XP_023528241.1 (structure-specific endonuclease subunit slx1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 233.4 bits (594), Expect = 1.7e-57
Identity = 118/169 (69.82%), Postives = 134/169 (79.29%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY 92
           MRLLS TFR  K PIS+ + S SSS+      TL  +S+ +PK WCVYLIISSN P KTY
Sbjct: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61

Query: 93  VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI 152
           VGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWKE+ RK+
Sbjct: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121

Query: 153 AHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 202
            H RK     E+ D+Q LRLLK RQRAL KVK +FDC++FEFD +LDPF
Sbjct: 122 CHMRK-----EEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165

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BLAST of Moc08g08690 vs. ExPASy Swiss-Prot
Match: B6JY16 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces japonicus (strain yFS275 / FY16936) OX=402676 GN=slx1 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-12
Identity = 33/71 (46.48%), Postives = 48/71 (67.61%), Query Frame = 0


Query: 77  WCVYLIISSNSPFKT-YVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQ 136
           +C YL++S  +  ++ Y+G T + +RRL+QHNGEIKGGA  T+  RPW  AC +HGF  +
Sbjct: 3   YCCYLLVSEKAASRSVYIGSTPDPARRLRQHNGEIKGGAYKTKRSRPWKVACFVHGFPTK 62

Query: 137 SQACEFESKWK 147
             A +FE  W+
Sbjct: 63  IAALQFEWVWQ 73

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BLAST of Moc08g08690 vs. ExPASy Swiss-Prot
Match: Q9P7M3 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=slx1 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.5e-11
Identity = 32/74 (43.24%), Postives = 48/74 (64.86%), Query Frame = 0


Query: 77  WCVYLIIS--SNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKD 136
           +C YL+ S  + S    Y+G T +  RRL+QHNGEI GGA  T+ GRPW  +C+++GF +
Sbjct: 7   YCCYLLKSNRTQSSGAVYIGSTPDPPRRLRQHNGEIVGGASKTKHGRPWSISCLVYGFPN 66

Query: 137 QSQACEFESKWKEV 149
           +  A +FE  W+ +
Sbjct: 67  KVSALKFEWNWQNL 80

[Close]

BLAST of Moc08g08690 vs. ExPASy Swiss-Prot
Match: B8MDD1 (Structure-specific endonuclease subunit slx1 OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=slx1 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-10
Identity = 38/89 (42.70%), Postives = 53/89 (59.55%), Query Frame = 0


Query: 65  TLSFESQL-KPKP--WCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG- 124
           T+S E+ L KP P  +C YL+ S+  P   Y+G T + +RRL+QHNG  KGGAK T    
Sbjct: 4   TVSEEAVLTKPIPAFYCCYLLRSAKRPSALYIGSTPDPARRLEQHNGFAKGGAKRTERNT 63

Query: 125 -RPWVCACMIHGFKDQSQACEFESKWKEV 149
            RPW    ++ GF  ++ A +FE  W+ V
Sbjct: 64  LRPWEMVAIVEGFPSRTGALQFEWSWQHV 92

[Close]

BLAST of Moc08g08690 vs. ExPASy Swiss-Prot
Match: A4I1H7 (Structure-specific endonuclease subunit SLX1 homolog OS=Leishmania infantum OX=5671 GN=LinJ25.1380 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.7e-10
Identity = 35/82 (42.68%), Postives = 48/82 (58.54%), Query Frame = 0


Query: 78  CVYLIISSNSPFK--TYVGVTLNFSRRLKQHNGEIKGGAKAT-RAGRPWVCACMIHGFKD 137
           CVYL+ S +   +   Y+G T+N  RRL+QHNGE+  GA+ T R GRPW   C + GF D
Sbjct: 7   CVYLLTSLDPQCEGDFYIGYTVNPLRRLRQHNGELVNGARRTSRRGRPWTIVCCVSGFPD 66

Query: 138 QSQACEFESKWKE--VSRKIAH 155
              A +FE  W+    S ++ H
Sbjct: 67  DRAALKFEWCWQHPTASARLRH 88

[Close]

BLAST of Moc08g08690 vs. ExPASy Swiss-Prot
Match: Q5CT62 (Structure-specific endonuclease subunit SLX1 homolog OS=Cryptosporidium parvum (strain Iowa II) OX=353152 GN=cgd2_4280 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-09
Identity = 29/67 (43.28%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 80  YLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQAC 139
           Y ++S      +Y+G ++N  RRL+QHNGEIK GAK T++G PW     + GF D+  A 
Sbjct: 11  YFLLSEAKKKASYIGYSVNPCRRLRQHNGEIKKGAKKTKSGVPWNLGICVGGFPDRVAAL 70

Query: 140 EFESKWK 147
            FE  W+
Sbjct: 71  RFEWAWQ 77

[Close]

BLAST of Moc08g08690 vs. ExPASy TrEMBL
Match: A0A6J1DGL6 (structure-specific endonuclease subunit SLX1 OS=Momordica charantia OX=3673 GN=LOC111020266 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 6.6e-108
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MKQIVVEHESTVNQTAKRQFPNRRQRRGTRERMRLLSHTFRCHKSPISNPKPSSSSSTKD 60
           MKQIVVEHESTVNQTAKRQFPNRRQRRGTRERMRLLSHTFRCHKSPISNPKPSSSSSTKD
Sbjct: 1   MKQIVVEHESTVNQTAKRQFPNRRQRRGTRERMRLLSHTFRCHKSPISNPKPSSSSSTKD 60

Query: 61  PSAATLSFESQLKPKPWCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG 120
           PSAATLSFESQLKPKPWCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG
Sbjct: 61  PSAATLSFESQLKPKPWCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG 120

Query: 121 RPWVCACMIHGFKDQSQACEFESKWKEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRAL 180
           RPWVCACMIHGFKDQSQACEFESKWKEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRAL
Sbjct: 121 RPWVCACMIHGFKDQSQACEFESKWKEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRAL 180

Query: 181 EKVKFMFDCSRFEFDGQLDPF 202
           EKVKFMFDCSRFEFDGQLDPF
Sbjct: 181 EKVKFMFDCSRFEFDGQLDPF 201

[Close]

BLAST of Moc08g08690 vs. ExPASy TrEMBL
Match: A0A1S3CFH5 (structure-specific endonuclease subunit slx1 OS=Cucumis melo OX=3656 GN=LOC103500344 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 2.7e-61
Identity = 127/171 (74.27%), Postives = 142/171 (83.04%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 92
           MRLLS TFRC K PISNP+   SSSSSTK P+      E   KPK WCVYLIISSNSP K
Sbjct: 1   MRLLSQTFRCQKPPISNPRLPKSSSSSTKGPTLIVKLEEP--KPKQWCVYLIISSNSPIK 60

Query: 93  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 152
           TYVGVTL+F RRLKQHNGEIKGGAKAT+AGRPW+CAC IHGFKDQSQACEFESKWK+VSR
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATQAGRPWICACTIHGFKDQSQACEFESKWKKVSR 120

Query: 153 KIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 202
           KI++++K+DD  +Q D Q LRLLK+RQRAL KVK +FDCS FEFD +LDPF
Sbjct: 121 KISYEKKEDDVGKQLDNQTLRLLKHRQRALGKVKCLFDCSHFEFDWKLDPF 169

[Close]

BLAST of Moc08g08690 vs. ExPASy TrEMBL
Match: A0A0A0LCZ9 (GIY-YIG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683170 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 2.7e-61
Identity = 128/173 (73.99%), Postives = 147/173 (84.97%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNP--KPSSSSSTKDPSAATLSFESQLKP--KPWCVYLIISSNSP 92
           MRLLSHTFRC K PISNP    SSSSS+KDP   TL  +S+ KP  K WCVYLIISSNSP
Sbjct: 1   MRLLSHTFRCVKPPISNPPLSKSSSSSSKDP---TLIVKSEPKPKLKQWCVYLIISSNSP 60

Query: 93  FKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEV 152
            KTYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWK+V
Sbjct: 61  IKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACTIHGFKDQSQACEFESKWKKV 120

Query: 153 SRKIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 202
           SRKI++++K++D  +  D+Q LRLLK+R+RAL KVK +FDCS+FEFD +LDPF
Sbjct: 121 SRKISYEKKEEDVGKLLDDQTLRLLKHRERALGKVKCLFDCSQFEFDWKLDPF 170

[Close]

BLAST of Moc08g08690 vs. ExPASy TrEMBL
Match: A0A6J1J5C9 (structure-specific endonuclease subunit slx1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481456 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 4.1e-57
Identity = 121/171 (70.76%), Postives = 139/171 (81.29%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 92
           MRLLS TFR  K PIS+P+   SSSSSTK P   TL+ +S+ + K WCVYLIISSN P K
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVP---TLTIKSEPETKKWCVYLIISSNPPIK 60

Query: 93  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 152
           TYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFK+QSQACEFESKWKE+ R
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKNQSQACEFESKWKEICR 120

Query: 153 KIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 202
           K++H RK     E+ D+Q LRLLK RQRAL KVK +FDC++FEFD +LDPF
Sbjct: 121 KMSHMRK-----EEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 163

[Close]

BLAST of Moc08g08690 vs. ExPASy TrEMBL
Match: A0A6J1F1V2 (structure-specific endonuclease subunit slx1 OS=Cucurbita moschata OX=3662 GN=LOC111441428 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 1.2e-56
Identity = 121/170 (71.18%), Postives = 136/170 (80.00%), Query Frame = 0


Query: 33  MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 92
           MRLLS TFR  K PIS+P+   SSSSSTK P   TL  +S+ +PK WCVYLIISSN P K
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVP---TLIVKSEPEPKKWCVYLIISSNPPIK 60

Query: 93  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 152
           TYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWKE+ R
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICR 120

Query: 153 KIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDP 201
           K+ H RK     E+ D+  LRLLK RQRAL KVK +FDC++FEFD +LDP
Sbjct: 121 KMCHMRK-----EEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 162

[Close]

BLAST of Moc08g08690 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 147.9 bits (372), Expect = 8.7e-36
Identity = 88/173 (50.87%), Postives = 115/173 (66.47%), Query Frame = 0


Query: 34  RLLSHTFRCHKSPISNPKPSSSSSTKD-------PSAATLSFESQL-KPKPWCVYLIISS 93
           RLLS TF   K   SNPK     + K+       PS+++    S++ K K W VYLI+S+
Sbjct: 3   RLLSRTFPSVKLRDSNPKAPEFKNLKNSQTQVPIPSSSSPQENSKIPKSKSWSVYLILST 62

Query: 94  NSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKW 153
             P KTYVG+T +FSRRLKQHNGEI+GGAKA+ AGRPW+CAC+I GF   SQA  FESKW
Sbjct: 63  TEPIKTYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTCLSQASSFESKW 122

Query: 154 KEVSRKIAHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQL 199
           K  +RK+  +RKKD++  Q+D     LL++R+RAL KV+   DCS  E D ++
Sbjct: 123 KIFTRKLP-RRKKDEDMSQSD----ALLQHRRRALNKVEESLDCSYLETDWKI 170

[Close]

BLAST of Moc08g08690 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-09
Identity = 31/76 (40.79%), Postives = 42/76 (55.26%), Query Frame = 0


Query: 80  YLIISSNSPFK--TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQ 139
           YL+ S +   K  TY+G T+N  RR++QHNGEI  GA  T+  RPW     I+GF     
Sbjct: 31  YLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRPWEMVLCIYGFPTNVS 90

Query: 140 ACEFESKWKEVSRKIA 154
           A +FE  W+     +A
Sbjct: 91  ALQFEWAWQHPRESVA 106

The following BLAST results are available for this feature:

BLAST of Moc08g08690 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152567.1	1.4e-107	100.00	structure-specific endonuclease subunit SLX1 [Momordica charantia]	[more]
XP_004151512.1	5.6e-61	73.99	structure-specific endonuclease subunit SLX1 [Cucumis sativus] >KGN58557.1 hypot...	[more]
XP_008461838.1	5.6e-61	74.27	PREDICTED: structure-specific endonuclease subunit slx1 [Cucumis melo] >XP_00846...	[more]
XP_038903984.1	6.9e-59	73.10	structure-specific endonuclease subunit slx1 isoform X1 [Benincasa hispida]	[more]
XP_023528241.1	1.7e-57	69.82	structure-specific endonuclease subunit slx1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc08g08690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6JY16	1.0e-12	46.48	Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces japonicus (s...	[more]
Q9P7M3	2.5e-11	43.24	Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces pombe (strai...	[more]
B8MDD1	2.1e-10	42.70	Structure-specific endonuclease subunit slx1 OS=Talaromyces stipitatus (strain A...	[more]
A4I1H7	4.7e-10	42.68	Structure-specific endonuclease subunit SLX1 homolog OS=Leishmania infantum OX=5...	[more]
Q5CT62	1.4e-09	43.28	Structure-specific endonuclease subunit SLX1 homolog OS=Cryptosporidium parvum (...	[more]

BLAST of Moc08g08690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGL6	6.6e-108	100.00	structure-specific endonuclease subunit SLX1 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A1S3CFH5	2.7e-61	74.27	structure-specific endonuclease subunit slx1 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A0A0LCZ9	2.7e-61	73.99	GIY-YIG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683170 PE=...	[more]
A0A6J1J5C9	4.1e-57	70.76	structure-specific endonuclease subunit slx1 isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1F1V2	1.2e-56	71.18	structure-specific endonuclease subunit slx1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of Moc08g08690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43210.1	8.7e-36	50.87	Excinuclease ABC, C subunit, N-terminal	[more]
AT2G30350.2	1.1e-09	40.79	Excinuclease ABC, C subunit, N-terminal	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 151..178
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..62
None	No IPR available	PANTHER	PTHR20208	STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1	coord: 49..200
None	No IPR available	PANTHER	PTHR20208:SF13	EMB\|CAB76036.1	coord: 49..200
None	No IPR available	CDD	cd10455	GIY-YIG_SLX1	coord: 77..146 e-value: 1.11986E-35 score: 118.489
IPR035901	GIY-YIG endonuclease superfamily	GENE3D	3.40.1440.10		coord: 68..182 e-value: 1.3E-24 score: 88.1
IPR035901	GIY-YIG endonuclease superfamily	SUPERFAMILY	82771	GIY-YIG endonuclease	coord: 76..152
IPR000305	GIY-YIG endonuclease	PFAM	PF01541	GIY-YIG	coord: 77..151 e-value: 7.5E-16 score: 58.2
IPR000305	GIY-YIG endonuclease	PROSITE	PS50164	GIY_YIG	coord: 75..160 score: 17.256058

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g08690.1	Moc08g08690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0033557	Slx1-Slx4 complex
molecular_function	GO:0017108	5'-flap endonuclease activity
molecular_function	GO:0008821	crossover junction endodeoxyribonuclease activity