Moc08g08330 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g08330
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDNA-directed RNA polymerase subunit
Locationchr8: 6177475 .. 6183327 (-)
RNA-Seq ExpressionMoc08g08330
SyntenyMoc08g08330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTATTCTCGTGGACACGACTTCTTGTTTTGCAACTTGTGTGGGACTATGCTATCTTTCAGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCCAGGCGTAGTGCAAAAGGTGGACATACCTTTCTTTAGCTATTTACTTTTAGTTTTTCATTTTGGATATCTGAAACATTCAATCACAAATTACTTGGATAAATGAGTGCTCAAGTTGTATACCTTGCAATAATTACACTCATTTTATTTGATCTTACTACATTATGTTGTGCAGTGGATTTTTCCTTCCTGTTTATCTACTTAATTAGTAAAAATGTGAAAGGCATCATTTTGATTTTCAGCTTTTATCACGAGTCTCTATTCTTTAATGATTAATGTTTCTACTAGTAGAAAAGAATTGTCTCCGGGTCGTGCAAGATGTTTTTTCTTCAATCTATCAATATAAATTTAAAGAATTCCTTGTGTAGTTAGGAGAGTTTCGATAAGGGAATTTCCACCATCTTCTCCTTTGAGTGTTCAACTTGAGTAGCTAATCACTTTCTTTTAAAAAGTTTATTTGATGAAGGTTAGCTATGTTTTCTTTGGTACCTTAGTTTAAATCAAAGCATGGGTAAGTTGACTAATATTGTTATGATGAACAACCTTCTTCCATTAGGACGAATGAAAGTTCAAAAAGGACCATCGATGGATGTAGAAAACTTTCCACTAAGTTTACTGCCATAGCTCTGGTCAATAAGTGGCTTAGGCCATCTACGGATGAGACAAAAAGAAACGAAGAAAGATGATCCCTTTGATGTAACCTTTTGTAGCCAAAACCTTTCATCTAGCTTTGCTATTCAAAAAAGAACCCAGAAGTCCATGGAGAAGAGACATCCTTCAATTCACGTTCTCCACCTCTGCCCAACTCATTAACCATAAGGATCAAGTCTATAATTTGGAAATTTTCAACAAAAGCCAGCTGAAATTTTGAGATCGTTCTAGGGTGGTCAGTTCTGATCCTGTTAGCAAAAACTTCAACAAGATTCTTATAAGGGCTTATAGTTAGGCTTATGGGTCATTGGGTCTATTGTCTTTCACTTGGCGTTATCCTCATTAGCCATAAGGGCAATATAAGTCTTGCTTATAATCACATTAACAATTTCATTTCTTAAAGAAGCTTGGAATGTTGACATGATATCATGCTTGAGAAAAAAATCCCACGACATTGTGATGAATTCTAAGGAGAAGCCATCTAGGTCCGTGGCTTTGCTCGCCAAGACAGGAAATAGCCCTTTGAACTTTATGCTAAATAAAAGCCTGCGGGATGAGAGCACCAGCTGTAGAGACAGGACTCCAAAATAAGCCATGGAAGATAAAGTACCACTACGAGTTATCCTCCAAAGACTGATTTTGGGTGGTTCTTTCAGAAAGGCTTAGTATGGTAGACTGCTGTGGTTTTGACGTTTCGAACTTCAGATTCAGAAGGCAGCTAACTGGTCTGTGGATGAATGAATAGGCTGAGTCATTCCTTATTTTGAAAGATTATTTCTCAAACTAGTAAAAGATGGACCTTGGAAACTCTAGATTGATTCCTTCCGAATCTTATTTCACCTTTCTCCCTAGTCCCTACCATGGATGAGCTCTGTAATTGTTTCATCGCAAAAGATATATGGAAGAAGAGATTACCCAAGTAGGTCAAATTCTTCGTTTGGACGTTGATCCTGGCTGGTAACAGTACAAATAAATTGCAACAAAGATTCCAATTTGAGCTAGTGTATCAGTGTATGTACGAGAAAACTTTTCCAGAATCAGAATCATCAGTTCAGTGACGAAGTTGCTTACAGATGTTGGGAAAAACTTTGAAGACCACCTATGGTCCTTAGCTGGTGTTTTCTTCACAACCCCAAGAATAATTATTTGTGCATGCTTTTTATGGACTATGGACGTCTTTTTTCTTATAGAACGTGTGCATCCTTTCAAGAAGGCAAGGATTATTGGGTGAATGAATGGGGTTTGAGCTATTTTGTGGGTGCTTTGGGTCGAGAGGAATGATAAAAGTTTTGATTCAGAAGTTTGTAGGATTTGTGGAGTCTGTTTTGTTTTTGGGTTTCTATTGGTGTACCCTTTAAGTTCTCTCTCTTTTGGTAGGTGAAACGATTTTGTTAAAAAAAATGAACTCGCAAAACAAGGAGAGGAAAGTTTTTAAAACATGTTGCAAGAACTAAAATGAATGTGCATTTTTTTTTTTTTGATAACCGGGAGTCAGAGCTTTGCAATTAAGCTTTTGCAACCTATTAGTTTTAAGCTTTTAGTTCTAATGATGATTCAATGTGGTTTCAAAGTAGTTAGTCTTAAACATCAAATTGTTGTAATGTTGTTTCCTCCCCCATTTAATATTTATAGAGTTTACATGTTTGGTTTCATACTAATATCCAAGCCCATAGGAGAAGGGAAGGGTTGAAGATGTATTGAAAGGATCTGAGTTCGCCTTGAATTTGCTCCTCATGGAGTTTTTTTGAAAAATTTTTGACTTTTTACGTTTCACAACCTGGATGCCTTGCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAACCACACTTTTCTTTTCATAAACATTGACTGGGAGCCTTTATTTATTTATTTTTGTATTAGCTTGGTTGCAGCAGGGTTGCCTCTACTCTGTCTTTAGGCTGCCTGTTCCCTTTCGTTCCTCTTAATATATTTTCTTAACCCAAAAGAAAATGCGTTCACCTACCTGCATAAACTTCTAGGTGCTGGTGTTTTAGTAGAAAACATCAATCCAAGCAAAGGCATATTATAGGAAAACTCTTCCCTAAGAAGCACGGACACGGACACGAGACACGGACATGGACACGACAGGACACGACAATACGTCAATTTCTAAAAAACTAGGACATGGACACGCTGGGGATACGTTATTAATTTATTATTTTTTTAGGATATAGGTAAATTTAACATAAAAATGCTAAATATACTTCGCTAAAAAACATAAATGTCAAGTAAATTTATTGGGTTGAAGATGTACCCACTAAAAAGCATAAAAATAGTAAATGTAATCCACCAAAAAAAATCCTAGAGAAAGATTTGAATTTGATTATTGGGTCGAAGTTGTCATCCTAATTTAGTAAAACATATTCTACTCCAGTTTAATTCATATGAAATTAGGGAAAAAAGGAAGTAAGAATAGAGCTTATTTAATTTAACTCACACAAACAGAAGGGTGGGTCACTAGGTAGCGTTTTAGGGTTTTTTTTTTTTTGTAGTTTAAGTTTTTGAGATTTCAGCCAAACTTCTTGGACTTCCAAAATTGGGCTAAGGAAACAATATTTTTTTATCTGGCGTGTCCCTCCCGTGTCCAAAACGTGTCCCCACGTGTCTAAAATTAAAATAAAAAATTTAATATTCGAATACTTTAAATTGCGTGTCGGACACGTGTCCGGAGCGTGTCCGGACGTATCCGTGTCCGACACGTGTCCGACACAGACACTCCTCCTTTTTTGCCGTGTCCGTGTTTTATAGACTCTTCCAAATGACACACAGTGCTTAGATTATAACTATAAAACGAAGCATATAGTTTGCAACCAAGTAAGAGCTACAAAGGTGAGGTTAACCAAAAAATGCCCAAAAGATTTTCTCATCATGTTGAAGATGTATCTATTTCTTTCTAGCCGAAGCTTTCAAAAGAAAGCTCTTATAAACTGCAACCATAAGATTTTCTCTCTAGTGAGTGAAATGTGAATGCCCCCAAAAGGTGATCATAAGGAAGTTGGTGTTGATACTAATAGGGAGGGAGGATGTGGTTCTAGAATTGAATAGCAAAAGCAAGAGGAAATGGTTCTAGAACTGATCACAATTAGAGATTTGTTATCCTTTGTTCGCATGAACCACCAACCCAGGGAAAGAATCCACCCTTTTCCTATTCCTTTTATCTGCCTTTTTTTTTTTGTAATTCAAGCCAATTAGGGTAGTTTTCTGCAAGCAGGTCTTTTGAGTGGATTAATGTCTCATCTCCTCCCATCATTTGCGTGCATTTTTTTATTAGTATAGTCTTTGTTTAAAAGAAAGAAAGTAACAGTGGAACATGAGTAGAGAAAATAGGAGAAGAAGCAGTATGTAATACTGCAATTATAGGAGAATTATGGTAGAGAGAGAGAGTCAAAATTACTTGCTGAACGTTTCCTTAAAGTAAATTCAGACGCACAAGTATTTAGCATCCCCCTGTTGAAACAGCAGATCTTGTAAGGATATGATTTGAGGCATTGATGAATACTCTACCAGAGGATTGAAGGAAACTGATAGAAGATGGTAGAATTAAGGTACATGTGGCGATAAAGATTCCACGCAAGTAACAGATTTTATTGATAGAATCAAATGTTTCTTTAATACAATTACATATTTACGTGAAGGTATTGCGGTGTATGTGGAACTAGTAACAGGACAGCTGAGCAGAGAGTGAAAAATATCGAACTCAATAAAAATATGAAACACACCAAACCATTGGGCGGGTTAGTCAAGGGTCAGCAAGTGTTGGATTATGAGCTCCCTCTATGTTATTCTTCTACTCTTTGAAGTTTGTGGTACATCAAATCATTTTAGAATTTCTTCAGGAAGTTAACTTGCTTTGATAGCTTCATGCACCAACAGTTAGTAGAAAGAAGCATGCATCTTATATTCTCTTGATGTATTAAAATTGAAAGAATTGAAATTTAATCCTTTACATTGTTCTTATCTTTGAGTTTATACTTGTACAAAATTCAGTGTGAGAAATTTTGTTCAAATATTTTGTGCACTTCTTTAGTAGACATCTCTAACCTTTGTGCGGCTTATTTCAGAGATCATCGGTAGAGAAATAAGTTACACAGTTACTGCTGAGGTATGCCTTTTAAATAATAAAAATTTTCCCGATGCTTGTTCTATTTGTAGGTCTATGACATCTTCCCCTCTGCTACTACGCATGTCATAGGCGATCTCTTTGAACTTAAGCAAATGATCTGTTAACATACTCTGAAAATTTCTGCTACACATGTGATATTTGCTAGAGGATCGTCTCTAGATGTACCCATCTCAATTATGGGTTCAGCTAAGGAATCCGTAAATGTTCACTAGGCTAGGCCAAATCGATAACCCCAACCTGAACAGGAAAATTCATATTGGGGCGTAAAAAAAAGACCTTGACTTCAACCCCCACCTCATATCTGTAAATTTTTTCTTTTATTTTTTTTTGGGGGGGGGGGGTGGATTTAATAAAAATTGTGCGAGGAGGTAGGTTTGGGGGAGTAGATGGATATTGTCGAAACCCAAGACTCTAGCCTTGGAAAACCACGGAGCCAAACATCCCTTTTATTGTATGCAAGTTTCAATGATCTAAGCTTTGATATGCCTCCCTTCTATTTGATTGTTAGACATCCAAGAAATCTCATAATCTGGAAATCCTTGTGTAATAATTTGCAGGAAATTAGAAAGCAGTTGGGGATTTCATTAATTGATGAAGAGAAAGTGCAGCTATCAAAGGTAATTGGGAGAAAGAAGTCTATGATATGATATTTTGCCTCTTGAGATGTGTAAGGATCCACTAATATCAGGCTGTGTCATGCTTTAACAGGAAAGGAGGAGATGTGAAAAATGTGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGGTACTTTATAGGCATAAAAAGACTCTAATGCCTGCCCTTTAGTCTTTCTTTCTGTCTCCCATTAAATAGAGTACTGAGTGCGAGTGTGGTTTCAATTTCTGCAGATGAGATCGGCTGATGAAGGGCAGACCACCTTTTATACCTGCACCAAGTGCCATCATCAGACCAGAGAGAATTGA

mRNA sequence

ATGGCTTATTCTCGTGGACACGACTTCTTGTTTTGCAACTTGTGTGGGACTATGCTATCTTTCAGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCCAGGCGTAGTGCAAAAGAGATCATCGGTAGAGAAATAAGTTACACAGTTACTGCTGAGGAAATTAGAAAGCAGTTGGGGATTTCATTAATTGATGAAGAGAAAGTGCAGCTATCAAAGGAAAGGAGGAGATGTGAAAAATGTGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGGCAGACCACCTTTTATACCTGCACCAAGTGCCATCATCAGACCAGAGAGAATTGA

Coding sequence (CDS)

ATGGCTTATTCTCGTGGACACGACTTCTTGTTTTGCAACTTGTGTGGGACTATGCTATCTTTCAGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCCAGGCGTAGTGCAAAAGAGATCATCGGTAGAGAAATAAGTTACACAGTTACTGCTGAGGAAATTAGAAAGCAGTTGGGGATTTCATTAATTGATGAAGAGAAAGTGCAGCTATCAAAGGAAAGGAGGAGATGTGAAAAATGTGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGGCAGACCACCTTTTATACCTGCACCAAGTGCCATCATCAGACCAGAGAGAATTGA

Protein sequence

MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLGISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Homology
[Close]
BLAST of Moc08g08330 vs. NCBI nr
Match: XP_022144336.1 (DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia] >XP_022144337.1 DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia])

HSP 1 Score: 239.2 bits (609), Expect = 1.8e-59
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. NCBI nr
Match: KAG6598510.1 (DNA-directed RNA polymerase I subunit RPA12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 237.7 bits (605), Expect = 5.2e-59
Identity = 114/116 (98.28%), Postives = 116/116 (100.00%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEI+GREISYTVTAEEIRKQLG
Sbjct: 31  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 90

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 91  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 146

[Close]
BLAST of Moc08g08330 vs. NCBI nr
Match: XP_023546814.1 (DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita pepo subsp. pepo] >KAG7029447.1 DNA-directed RNA polymerase I subunit RPA12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 237.7 bits (605), Expect = 5.2e-59
Identity = 114/116 (98.28%), Postives = 116/116 (100.00%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEI+GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. NCBI nr
Match: XP_022997634.1 (DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima] >XP_022997635.1 DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima])

HSP 1 Score: 235.3 bits (599), Expect = 2.6e-58
Identity = 114/116 (98.28%), Postives = 115/116 (99.14%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK IIGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKGIIGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. NCBI nr
Match: XP_038886279.1 (DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] >XP_038886280.1 DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] >XP_038886281.1 DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] >XP_038886282.1 DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida])

HSP 1 Score: 235.3 bits (599), Expect = 2.6e-58
Identity = 113/116 (97.41%), Postives = 116/116 (100.00%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSFSSTKYV+CPLCKS+RSAKEIIGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVQCPLCKSQRSAKEIIGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. ExPASy Swiss-Prot
Match: Q5TM50 (DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta OX=9544 GN=POLR1H PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 5.5e-11
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 0 

Query: 8   DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLGISLIDE 67
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCTRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 68  EKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]
BLAST of Moc08g08330 vs. ExPASy Swiss-Prot
Match: Q1RMP0 (DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus OX=9913 GN=POLR1H PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 9.3e-11
Identity = 37/110 (33.64%), Postives = 60/110 (54.55%), Query Frame = 0 

Query: 8   DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLGISLIDE 67
           D  FC  CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 13  DLDFCPDCGSVLPLPGVQDAVACTRCGFSINVRDFEGKVVKTSVVFNKLGTAMPLSMEEG 72

Query: 68  EKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 73  PEFQGPVVDRRCSRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 122

[Close]
BLAST of Moc08g08330 vs. ExPASy Swiss-Prot
Match: Q9P1U0 (DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens OX=9606 GN=POLR1H PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 9.3e-11
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 0 

Query: 8   DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLGISLIDE 67
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 68  EKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]
BLAST of Moc08g08330 vs. ExPASy Swiss-Prot
Match: Q1XHV8 (DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes OX=9598 GN=POLR1H PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 9.3e-11
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 0 

Query: 8   DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLGISLIDE 67
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 68  EKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]
BLAST of Moc08g08330 vs. ExPASy Swiss-Prot
Match: O94703 (DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpa12 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.0e-10
Identity = 32/111 (28.83%), Postives = 59/111 (53.15%), Query Frame = 0 

Query: 10  LFCNLCGTMLSFSSTKYVECPLCK----SRRSAKEIIGREISYTVTAEEIRKQLGISLID 69
           +FC+ CG +L  ++ ++  C  C+    S + A  ++  + S +     ++ +  I  ++
Sbjct: 8   IFCSECGNLLESTTAQWTTCDQCQSVYPSEQFANLVVETKSSASAFPSALKLKHSIVQVE 67

Query: 70  EEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
            +K + +    +C KCGND   F + Q+RSADEG T FY C +C ++   N
Sbjct: 68  SQKEEAATIEEKCPKCGNDHMTFHTLQLRSADEGSTVFYECPRCAYKFSTN 118

[Close]
BLAST of Moc08g08330 vs. ExPASy TrEMBL
Match: A0A6J1CTE6 (DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC111014043 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 8.6e-60
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. ExPASy TrEMBL
Match: A0A6J1KC19 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111492509 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.2e-58
Identity = 114/116 (98.28%), Postives = 115/116 (99.14%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK IIGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKGIIGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. ExPASy TrEMBL
Match: A0A6J1HDS4 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111463020 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.6e-58
Identity = 113/116 (97.41%), Postives = 115/116 (99.14%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYS GHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEI+GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSGGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. ExPASy TrEMBL
Match: A0A0A0LKC4 (DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_2G345940 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-57
Identity = 111/116 (95.69%), Postives = 114/116 (98.28%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSF STKYVECP CKSRRSAKEI+GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLIDEEK+QL+KERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. ExPASy TrEMBL
Match: A0A1S3BBZ4 (DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103488399 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.5e-56
Identity = 110/116 (94.83%), Postives = 113/116 (97.41%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           MAYSRGHDFLFCNLCGTMLSF STKYVECPLCKS+RSAKEI GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSKRSAKEINGREISYTVTAEEIRKQLG 60

Query: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 117
           ISLI EEK+QL+KERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIGEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Moc08g08330 vs. TAIR 10
Match: AT3G25940.1 (TFIIB zinc-binding protein )

HSP 1 Score: 134.4 bits (337), Expect = 5.8e-32
Identity = 67/118 (56.78%), Postives = 87/118 (73.73%), Query Frame = 0 

Query: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
           M  SR  +FLFCNLCGTML   STKY ECP CK+ R+AK+II +EI+YTV+AE+IR++LG
Sbjct: 1   MEKSRESEFLFCNLCGTMLVLKSTKYAECPHCKTTRNAKDIIDKEIAYTVSAEDIRRELG 60

Query: 61  ISLIDEE---KVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 116
           ISL  E+   + +L K ++ CEKC + E  + +RQ RSADEGQTT+YTC  C H+  E
Sbjct: 61  ISLFGEKTQAEAELPKIKKACEKCQHPELVYTTRQTRSADEGQTTYYTCPNCAHRFTE 118

[Close]
BLAST of Moc08g08330 vs. TAIR 10
Match: AT1G20065.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: TFIIB zinc-binding protein (TAIR:AT3G25940.1). )

HSP 1 Score: 85.9 bits (211), Expect = 2.4e-17
Identity = 39/62 (62.90%), Postives = 48/62 (77.42%), Query Frame = 0 

Query: 1  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60
          M  SR  D LFCNLCGTML   STKY ECPLC++ R+ KEII + ++YTVT E+IR++LG
Sbjct: 1  MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGKEIIDKNLAYTVTTEDIRRELG 60

Query: 61 IS 63
          IS
Sbjct: 61 IS 62

[Close]
BLAST of Moc08g08330 vs. TAIR 10
Match: AT1G20065.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: TFIIB zinc-binding protein (TAIR:AT3G25940.1). )

HSP 1 Score: 73.6 bits (179), Expect = 1.2e-13
Identity = 33/53 (62.26%), Postives = 39/53 (73.58%), Query Frame = 0 

Query: 1  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAE 54
          M  SR  D LFCNLCGTML   STKY ECPLC++ R+ KEII + ++YTVT E
Sbjct: 1  MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGKEIIDKNLAYTVTTE 53

[Close]
BLAST of Moc08g08330 vs. TAIR 10
Match: AT4G07950.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 49.7 bits (117), Expect = 1.9e-06
Identity = 31/110 (28.18%), Postives = 56/110 (50.91%), Query Frame = 0 

Query: 11  FCNLCGTMLSF---SSTKYV--ECPLCKSRRSAKEIIGREISYTVTAEEIRKQLGISLID 70
           FC  CG +L +    S+++    CP   +     EI  +++        ++K +   +  
Sbjct: 3   FCPTCGNLLRYEGGGSSRFFCSTCPYVANIERRVEIKKKQLL-------VKKSIEPVVTK 62

Query: 71  EEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 116
           ++    ++    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  DDIPTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

[Close]
BLAST of Moc08g08330 vs. TAIR 10
Match: AT1G01210.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 49.3 bits (116), Expect = 2.4e-06
Identity = 30/107 (28.04%), Postives = 50/107 (46.73%), Query Frame = 0 

Query: 11  FCNLCGTMLSF--SSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLGISLIDEEK 70
           FC  CG +L +         C  C         I R++        ++K +   +  ++ 
Sbjct: 3   FCPTCGNLLRYEGGGNSRFFCSTC----PYVAYIQRQVEIKKKQLLVKKSIEAVVTKDDI 62

Query: 71  VQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 116
              ++    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  PTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

The following BLAST results are available for this feature:
BLAST of Moc08g08330 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022144336.11.8e-59100.00DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia] >XP_02214... [more]
KAG6598510.15.2e-5998.28DNA-directed RNA polymerase I subunit RPA12, partial [Cucurbita argyrosperma sub... [more]
XP_023546814.15.2e-5998.28DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita pepo subsp. pepo] >K... [more]
XP_022997634.12.6e-5898.28DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima] >XP_02299763... [more]
XP_038886279.12.6e-5897.41DNA-directed RNA polymerase I subunit RPA12-like isoform X1 [Benincasa hispida] ... [more]
BLAST of Moc08g08330 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q5TM505.5e-1133.64DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta OX=9544 GN=POLR1H ... [more]
Q1RMP09.3e-1133.64DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus OX=9913 GN=POLR1H PE=2... [more]
Q9P1U09.3e-1133.64DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens OX=9606 GN=POLR1H PE... [more]
Q1XHV89.3e-1133.64DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes OX=9598 GN=POLR1H... [more]
O947036.0e-1028.83DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain... [more]
BLAST of Moc08g08330 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CTE68.6e-60100.00DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC1110140... [more]
A0A6J1KC191.2e-5898.28DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111492509 ... [more]
A0A6J1HDS41.6e-5897.41DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC11146302... [more]
A0A0A0LKC41.1e-5795.69DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_2G345940 P... [more]
A0A1S3BBZ41.5e-5694.83DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103488399 PE=3... [more]
BLAST of Moc08g08330 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G25940.15.8e-3256.78TFIIB zinc-binding protein [more]
AT1G20065.12.4e-1762.90FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT1G20065.21.2e-1362.26FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT4G07950.11.9e-0628.18DNA-directed RNA polymerase, subunit M, archaeal [more]
AT1G01210.12.4e-0628.04DNA-directed RNA polymerase, subunit M, archaeal [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001222Zinc finger, TFIIS-typeSMARTSM00440Cys4_2coord: 76..115
e-value: 3.7E-12
score: 56.4
IPR001222Zinc finger, TFIIS-typePFAMPF01096TFIIS_Ccoord: 77..112
e-value: 8.5E-16
score: 57.5
IPR001222Zinc finger, TFIIS-typePROSITEPS51133ZF_TFIIS_2coord: 74..114
score: 12.826397
IPR012164DNA-directed RNA polymerase subunit/transcription factor SPIRSFPIRSF005586RNApol_RpoMcoord: 9..116
e-value: 3.0E-27
score: 93.3
IPR012164DNA-directed RNA polymerase subunit/transcription factor SPANTHERPTHR11239DNA-DIRECTED RNA POLYMERASEcoord: 1..114
NoneNo IPR availableGENE3D2.20.25.10coord: 62..116
e-value: 1.7E-16
score: 61.4
NoneNo IPR availablePANTHERPTHR11239:SF19DNA-DIRECTED RNA POLYMERASE SUBUNITcoord: 1..114
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 55..116
IPR034004Pol I subunit A12, C-terminal zinc ribbonCDDcd10507Zn-ribbon_RPA12coord: 76..116
e-value: 9.60256E-21
score: 76.4728

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g08330.1Moc08g08330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006379 mRNA cleavage
biological_process GO:0006363 termination of RNA polymerase I transcription
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding