Moc08g06250 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc08g06250 vs. NCBI nr
Match: XP_022131596.1 (subtilisin-like protease SBT1.7 [Momordica charantia])

HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 775/775 (100.00%), Postives = 775/775 (100.00%), Query Frame = 0

Query: 1   MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ 60
           MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ
Sbjct: 1   MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ 60

Query: 61  WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 120
           WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT
Sbjct: 61  WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 120

Query: 121 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 180
           PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF
Sbjct: 121 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 180

Query: 181 SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF 240
           SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF
Sbjct: 181 SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF 240

Query: 241 GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR 300
           GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR
Sbjct: 241 GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR 300

Query: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK 360
           DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK
Sbjct: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK 360

Query: 361 KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV 420
           KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV
Sbjct: 361 KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV 420

Query: 421 QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF 480
           QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF
Sbjct: 421 QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF 480

Query: 481 STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 540
           STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR
Sbjct: 481 STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 540

Query: 541 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 600
           RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG
Sbjct: 541 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 600

Query: 601 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK 660
           LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK
Sbjct: 601 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK 660

Query: 661 NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI 720
           NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI
Sbjct: 661 NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI 720

Query: 721 VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of Moc08g06250 vs. NCBI nr
Match: XP_038885098.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1369.8 bits (3544), Expect = 0.0e+00
Identity = 686/772 (88.86%), Postives = 730/772 (94.56%), Query Frame = 0

Query: 4   MKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYD 63
           MKMQTCR+S+ FLLFLI F S S+  A+K+N++LKKKTY+IHMDRTNMPQAFDDHF+WYD
Sbjct: 1   MKMQTCRLSQWFLLFLISFCSCSFTEAQKSNRQLKKKTYVIHMDRTNMPQAFDDHFKWYD 60

Query: 64  SSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEF 123
           SSLKSVS+SAQMLYSY+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KYELHTTRTPEF
Sbjct: 61  SSLKSVSNSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEF 120

Query: 124 LGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSS 183
           LGLGKS SFFPAS KV EVIIG+LDTGVWPELESFNDAGLGPVP SWKGECEVGKNF+SS
Sbjct: 121 LGLGKSVSFFPASEKVSEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSS 180

Query: 184 SCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 244 SGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV 303
           +GTARGMAA+ARVATYKVCWLGGCF SDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTARGMAAEARVATYKVCWLGGCFSSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFT 363
           AIGAFSAAAQGVFVSCSAGNGGPSSG+LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK T
Sbjct: 301 AIGAFSAAAQGVFVSCSAGNGGPSSGTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKIT 360

Query: 364 GESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKG 423
           GESLYSGKPLPDSL+PIVYA +ASNSTSGS CL+ TLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GESLYSGKPLPDSLLPIVYAGNASNSTSGSLCLSSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTG 483
           +VVKEAGG GMILANTE YGEE+LADAHL P AAVG+K+GDAIKSYISSDANPTA  STG
Sbjct: 421 LVVKEAGGVGMILANTEAYGEEQLADAHLTPTAAVGKKSGDAIKSYISSDANPTATISTG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVA 543
           TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTG AGPTGLD DKR VA
Sbjct: 481 TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPS 603
           FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQD+S+G PS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEVIQDVSNGSPS 600

Query: 604 TPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYK 663
           TPFDIGAGHVNP AALDPGLVYDTTTDDYF FLCALNY+SLQIKVI+K+DFTC+ +KNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVISKRDFTCNGNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVE 723
           LEDLNYPSFAVPLETPS RGG D APTTVKYTRTLTNK A STYKVS T+    VKIVVE
Sbjct: 661 LEDLNYPSFAVPLETPSTRGGEDVAPTTVKYTRTLTNKGAPSTYKVSVTAPK-EVKIVVE 720

Query: 724 PESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           PESLSFAE NEQKSYTVTFIASPMPSG+ESFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 PESLSFAEANEQKSYTVTFIASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 771

BLAST of Moc08g06250 vs. NCBI nr
Match: XP_004142884.1 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KGN62460.1 hypothetical protein Csa_018794 [Cucumis sativus])

HSP 1 Score: 1357.0 bits (3511), Expect = 0.0e+00
Identity = 677/770 (87.92%), Postives = 725/770 (94.16%), Query Frame = 0

Query: 6   MQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 65
           M+TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+TNMPQAFDDHFQWYDSS
Sbjct: 1   MKTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSS 60

Query: 66  LKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLG 125
           LKSVSDSAQMLYSY+TVIHGFSTRLTVEEA+LMEK+EGI+AVIPE KYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLG 120

Query: 126 LGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 185
           LGKS SFFPAS KV EVIIG+LDTGVWPELESF+DAGLGP+P SWKGECEVGKNF+SS+C
Sbjct: 121 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNC 180

Query: 186 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 245
           NRKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+G
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 246 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 305
           TARGMAA+ARVATYKVCWLGGCF SDILAA+DK+VEDG N+LS+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAI 300

Query: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 365
           GAFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGE
Sbjct: 301 GAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 366 SLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVV 425
           SLYSGKPLP+SL+PIV AA ASNS+SGS CL+GTLNPAKV GKIVVCDRGGNSRVQKGVV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVV 420

Query: 426 VKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTT 485
           VKEAGG GMILANTE YGEE+LADAHL+P AAVGQK GDAIK+YISSD+NPTA  STGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTT 480

Query: 486 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 545
           RLGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 546 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 605
           IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTP
Sbjct: 541 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTP 600

Query: 606 FDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLE 665
           FDIGAGHVNP AALDPGLVYDTTTDDY  FLCALNY+SLQIKVI+KKDFTC+ +KNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 666 DLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPE 725
           DLNYPSFAVPLETPS RGG + APTT+KYTRTLTNK ASSTYKVS T+KS SVKIVVEPE
Sbjct: 661 DLNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPE 720

Query: 726 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           SLSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Moc08g06250 vs. NCBI nr
Match: KAG7029314.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 678/773 (87.71%), Postives = 726/773 (93.92%), Query Frame = 0

Query: 4   MKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYD 63
           MK+QTCRVS+ FLLFLI   S S+  A+K++Q+LKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 64  SSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEF 123
           SSLKSVSDSAQ LY Y+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KY+LHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 124 LGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSS 183
           LGL KS SFFPASVKVGEVI+G+LDTGV PELESF+D GLGPVP SWKGECEVGKNFSSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 184 SCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 244 SGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV 303
           +GTA+GMAA+ARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFT 363
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP Y+TLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 364 GESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKG 423
           G+SLY+GKPL DSL+PIVYAAHASNSTSGS CLT TLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTG 483
           +VVKEAGGAGMILANTETYGEE+LADAHLLPAAAVGQKTGDAIKSYIS++ANPTA  S G
Sbjct: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVA 543
           TTRLGVQPSP+VAAFSSRGPNLLTP ILKPDLIAPGVNILAGW G  GPTGLD DKR VA
Sbjct: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPS 603
           FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GEAIQD+SSGLPS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 604 TPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYK 663
           TPFDIGAGHVNP AALDPGLVYD T +DYF FLCALNY+S QIKVI+KKDFTCS +KNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSVRGGGD-AAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVV 723
           LEDLNYPSFAV LETPS +GGG+  APTTVKYTRTLTNK A STYKVS TSKSPSVKI+V
Sbjct: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 724 EPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           EPESLSFA+ NEQKSYTVTF+AS MPSG+ESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Moc08g06250 vs. NCBI nr
Match: KAG6598344.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1351.7 bits (3497), Expect = 0.0e+00
Identity = 678/773 (87.71%), Postives = 726/773 (93.92%), Query Frame = 0

Query: 4   MKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYD 63
           MK+QTCRVS+ FLLFLI   S S+  A+K++Q+LKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 64  SSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEF 123
           SSLKSVSDSAQ LY Y+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KY+LHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 124 LGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSS 183
           LGL KS SFFPASVKVGEVI+G+LDTGV PELESF+D GLGPVP SWKGECEVGKNFSSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 184 SCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 244 SGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV 303
           +GTA+GMAA+ARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFT 363
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP Y+TLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 364 GESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKG 423
           G+SLY+GKPL DSL+PIVYAAHASNSTSGS CLT TLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTG 483
           +VVKEAGGAGMILANTETYGEE+LADAHLLPAAAVGQKTGDAIKSYIS++ANPTA  S G
Sbjct: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVA 543
           TTRLGVQPSP+VAAFSSRGPNLLTP ILKPDLIAPGVNILAGW G  GPTGLD DKR VA
Sbjct: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPS 603
           FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GEAIQD+SSGLPS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 604 TPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYK 663
           TPFDIGAGHVNP AALDPGLVYD T +DYF FLCALNY+S QIKVI+KKDFTCS +KNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSRNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSVRGGGD-AAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVV 723
           LEDLNYPSFAV LETPS +GGG+  APTTVKYTRTLTNK A STYKVS TSKSPSVKI+V
Sbjct: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 724 EPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           EPESLSFA+ NEQKSYTVTF+AS MPSG+ESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Moc08g06250 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 6.4e-289
Identity = 494/760 (65.00%), Postives = 602/760 (79.21%), Query Frame = 0

Query: 18  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLY 77
           FL++   + +V +  ++Q     TYI+HM ++ MP +FD H  WYDSSL+S+SDSA++LY
Sbjct: 12  FLLLCLGFCHVSSSSSDQ----GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLY 71

Query: 78  SYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGK-SDSFFPAS 137
           +Y   IHGFSTRLT EEA+ +  + G+++V+PE +YELHTTRTP FLGL + +   FP +
Sbjct: 72  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 131

Query: 138 VKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFA 197
               +V++G+LDTGVWPE +S++D G GP+P+SWKG CE G NF++S CNRKLIGAR+FA
Sbjct: 132 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 191

Query: 198 KGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARV 257
           +GYES  GPIDES+ES+SP+DDDGHGTHTS+TAAGS V GA+L GYASGTARGMA +ARV
Sbjct: 192 RGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARV 251

Query: 258 ATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVF 317
           A YKVCWLGGCF SDILAAIDKA+ D VNVLS+SLGG   DYYRD VAIGAF+A  +G+ 
Sbjct: 252 AVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGIL 311

Query: 318 VSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDS 377
           VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFPA   LGNGK FTG SL+ G+ LPD 
Sbjct: 312 VSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDK 371

Query: 378 LVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMIL 437
           L+P +YA +ASN+T+G+ C+TGTL P KV GKIV+CDRG N+RVQKG VVK AGG GMIL
Sbjct: 372 LLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMIL 431

Query: 438 ANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVA 497
           ANT   GEE +ADAHLLPA  VG+K GD I+ Y+++D NPTA  S   T +GV+PSPVVA
Sbjct: 432 ANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVA 491

Query: 498 AFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPH 557
           AFSSRGPN +TP+ILKPDLIAPGVNILA WTGAAGPTGL  D RRV FNIISGTSMSCPH
Sbjct: 492 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 551

Query: 558 ISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPG 617
           +SGLAAL+K+ HP+WSPAAIRSALMTTAY TYK+G+ + DI++G PSTPFD GAGHV+P 
Sbjct: 552 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPT 611

Query: 618 AALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPL 677
            A +PGL+YD TT+DY  FLCALNYTS QI+ ++++++TC  SK+Y + DLNYPSFAV  
Sbjct: 612 TATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV-- 671

Query: 678 ETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPESLSFAEENEQK 737
              +V G G       KYTRT+T+   + TY V  TS++  VKI VEP  L+F E NE+K
Sbjct: 672 ---NVDGVG-----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 731

Query: 738 SYTVTF-IASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           SYTVTF + S  PSG+ SF  +EWSDGKH+VGSP+A +WT
Sbjct: 732 SYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Moc08g06250 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 3.3e-221
Identity = 417/741 (56.28%), Postives = 513/741 (69.23%), Query Frame = 0

Query: 39  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAE-L 98
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y+T  HGFS  L   EA+ L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 99  MEKREGILAVIPEAKYELHTTRTPEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELES 158
           +     IL +  +  Y LHTTRTPEFLGL               VIIG+LDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 159 FNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFAKGYESAF-GPIDESQESKSPK 218
           F+D  +  +P+ WKGECE G +F S  CN+KLIGAR F+KG++ A  G     +ES SP+
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 219 DDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARVATYKVCWLGGCFGSDILAAI 278
           D DGHGTHTSTTAAGSAV  A+  GYA+GTARGMA +ARVATYKVCW  GCFGSDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 279 DKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 338
           D+A+ DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 339 WITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCL 398
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  +  NS+S + CL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 399 TGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEELADAHLLPAA 458
            G+L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 459 AVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLI 518
           AVG+KTGD ++ Y+ SD+ PTA      T L V+PSPVVAAFSSRGPN +TP ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 519 APGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAI 578
            PGVNILAGW+ A GPTGLD D RR  FNI+SGTSMSCPHISGLA L+KAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 579 RSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFL 638
           +SALMTTAY        + D +    S P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 639 CALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTR 698
           C+L+YT   I  I K+     + K      LNYPSF       SV  GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF-------SVLFGG---KRVVRYTR 686

Query: 699 TLTN-KAASSTYKVSETSKSPSVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTE--S 758
            +TN  AASS YKV+  + +PSV I V+P  LSF    E+K YTVTF++    S T    
Sbjct: 687 EVTNVGAASSVYKVT-VNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 759 FARLEWSDGKHIVGSPIAFTW 775
           F  + WS+ +H V SP+AF+W
Sbjct: 747 FGSITWSNPQHEVRSPVAFSW 751

BLAST of Moc08g06250 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 8.5e-217
Identity = 403/775 (52.00%), Postives = 519/775 (66.97%), Query Frame = 0

Query: 17  LFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 76
           LF+I+  +  ++ AE T Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 77  ---DSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLG 136
              ++ ++LY+Y T  HG + +LT EEAE +E+ +G++AVIPE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 137 KSDS--FFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 196
           + +S   +   V   +V++G+LDTG+WPE ESFND G+ PVP +W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 197 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 256
           NRK++GAR F +GYE+A G IDE  E KSP+D DGHGTHT+ T AGS V GANLFG+A G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 257 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 316
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 317 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 376
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA + +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 377 SLYSGKPL--PDSLVPIVY-AAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQK 436
           SLY G+ +   +   P+VY   +AS+    S CL G L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 437 GVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFST 496
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  +    TA    
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 497 GTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRV 556
             TR+G++PSPVVAAFSSRGPN L+  ILKPDL+APGVNILA WTG   P+ L  D RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 557 AFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLP 616
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 617 STPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAK-KDFTCSASKN 676
           S+P+D GAGH++P  A DPGLVYD    +YF FLC  + +  Q+KV  K  + TC  +  
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 677 YKLEDLNYPSF-AVPLETPSVRGGGDAAPTTVKYTRTLTNKAAS-STYKVSETSKSPSVK 736
               +LNYP+  A+  E   V+         +   RT+TN     S+YKVS  S      
Sbjct: 672 KNPGNLNYPAISALFPENTHVK--------AMTLRRTVTNVGPHISSYKVS-VSPFKGAS 731

Query: 737 IVVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTW 775
           + V+P++L+F  ++++ SYTVTF  +        F  L W    H V SP+  TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Moc08g06250 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 1.3e-209
Identity = 398/788 (50.51%), Postives = 522/788 (66.24%), Query Frame = 0

Query: 6   MQTCRVSRLFLLF--LIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYD 65
           M    +S +F +F  L+ F+S S   ++        ++YI+H+ R++ P  F  H  W+ 
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGL------ESYIVHVQRSHKPSLFSSHNNWHV 60

Query: 66  SSLKSVSDSAQ---MLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 125
           S L+S+  S Q   +LYSYS  +HGFS RL+  +   + +   +++VIP+   E+HTT T
Sbjct: 61  SLLRSLPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHT 120

Query: 126 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 185
           P FLG  ++   +  S    +VI+G+LDTG+WPE  SF+D+GLGP+P++WKGECE+G +F
Sbjct: 121 PAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 186 SSSSCNRKLIGARYFAKGYESAFGPIDE--SQESKSPKDDDGHGTHTSTTAAGSAVTGAN 245
            +SSCNRKLIGAR F +GY +      +  ++ES+SP+D +GHGTHT++TAAGS V  A+
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 246 LFGYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLG--GSSP 305
           L+ YA GTA GMA++AR+A YK+CW GGC+ SDILAA+D+AV DGV+V+SLS+G  GS+P
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 306 DYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITL 365
           +Y+ D++AIGAF A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 366 GNGKKFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGG 425
           G+GK FTG SLY+G+ LPDS + +VY    S       C  G LN + V GKIV+CDRGG
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGG 420

Query: 426 NSRVQKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANP 485
           N+RV+KG  VK AGGAGMILANT   GEE  AD+HL+PA  VG K GD I+ YI +  +P
Sbjct: 421 NARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSP 480

Query: 486 TAKFSTGTTRLGVQ-PSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGL 545
           TAK S   T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT L
Sbjct: 481 TAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDL 540

Query: 546 DGDKRRVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQ 605
           D D RRV FNIISGTSMSCPH+SGLAAL++ AHPDWSPAAI+SAL+TTAY    +GE I+
Sbjct: 541 DIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIE 600

Query: 606 DISSGLPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDF- 665
           D+++G  S  F  GAGHV+P  AL+PGLVYD    +Y  FLCA+ Y    I V  +    
Sbjct: 601 DLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTL 660

Query: 666 --TCSASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSET 725
              C  SK     DLNYPSF+V   +             VKY R + N  ++        
Sbjct: 661 YDACETSKLRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVG 720

Query: 726 SKSP-SVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTES-----FARLEWSDGKHIV 775
            KSP +V+I V P  L+F++E     Y VTF +  +  G  S     F  +EW+DG+H+V
Sbjct: 721 VKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVV 769

BLAST of Moc08g06250 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 5.2e-198
Identity = 377/774 (48.71%), Postives = 497/774 (64.21%), Query Frame = 0

Query: 18  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDS-AQML 77
           F   F+  +      +       TYI+H+D    P  F  HF WY SSL S++ S   ++
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSII 63

Query: 78  YSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGKSD--SFFP 137
           ++Y TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  +D      
Sbjct: 64  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 123

Query: 138 ASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARY 197
            S    +++IG++DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S+CNRKL+GAR+
Sbjct: 124 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 183

Query: 198 FAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQA 257
           F  GYE+  G ++E+ E +SP+D DGHGTHT++ +AG  V  A+  GYA G A GMA +A
Sbjct: 184 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 243

Query: 258 RVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQG 317
           R+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    YY D +AIGAF A  +G
Sbjct: 244 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRG 303

Query: 318 VFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPL- 377
           +FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA + LGNGK  +G S+Y G  L 
Sbjct: 304 IFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLD 363

Query: 378 PDSLVPIVYAAH--ASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGG 437
           P  + P+VY       +  S S CL G+L+P  V GKIV+CDRG NSR  KG +V++ GG
Sbjct: 364 PGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGG 423

Query: 438 AGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYIS------SDANPTAKFSTGTT 497
            GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTA      T
Sbjct: 424 LGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGT 483

Query: 498 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 557
           RLG++P+PVVA+FS+RGPN  TP ILKPD+IAPG+NILA W    GP+G+  D RR  FN
Sbjct: 484 RLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFN 543

Query: 558 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 617
           I+SGTSM+CPH+SGLAAL+KAAHPDWSPAAIRSAL+TTAY+   +GE + D S+G  S+ 
Sbjct: 544 ILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSV 603

Query: 618 FDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNY-KL 677
            D G+GHV+P  A+DPGLVYD T+ DY  FLC  NYT   I  I ++   C  ++    +
Sbjct: 604 MDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV 663

Query: 678 EDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEP 737
            +LNYPSF+V  +       G++  +T  + RT+TN   S +    +        + VEP
Sbjct: 664 GNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEP 723

Query: 738 ESLSFAEENEQKSY-----TVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 774
           E LSF    ++ S+     T     SP  +  E+   + WSDGK  V SP+  T
Sbjct: 724 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET-GHIVWSDGKRNVTSPLVVT 770

BLAST of Moc08g06250 vs. ExPASy TrEMBL
Match: A0A6J1BPX8 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111004737 PE=3 SV=1)

HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 775/775 (100.00%), Postives = 775/775 (100.00%), Query Frame = 0

Query: 1   MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ 60
           MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ
Sbjct: 1   MKKMKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQ 60

Query: 61  WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 120
           WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT
Sbjct: 61  WYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 120

Query: 121 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 180
           PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF
Sbjct: 121 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 180

Query: 181 SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF 240
           SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF
Sbjct: 181 SSSSCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLF 240

Query: 241 GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR 300
           GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR
Sbjct: 241 GYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYR 300

Query: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK 360
           DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK
Sbjct: 301 DNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGK 360

Query: 361 KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV 420
           KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV
Sbjct: 361 KFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRV 420

Query: 421 QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF 480
           QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF
Sbjct: 421 QKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKF 480

Query: 481 STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 540
           STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR
Sbjct: 481 STGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKR 540

Query: 541 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 600
           RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG
Sbjct: 541 RVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSG 600

Query: 601 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK 660
           LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK
Sbjct: 601 LPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASK 660

Query: 661 NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI 720
           NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI
Sbjct: 661 NYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKI 720

Query: 721 VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 VVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of Moc08g06250 vs. ExPASy TrEMBL
Match: A0A0A0LKN0 (Xylem serine proteinase 1 OS=Cucumis sativus OX=3659 GN=Csa_2G354860 PE=3 SV=1)

HSP 1 Score: 1357.0 bits (3511), Expect = 0.0e+00
Identity = 677/770 (87.92%), Postives = 725/770 (94.16%), Query Frame = 0

Query: 6   MQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 65
           M+TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+TNMPQAFDDHFQWYDSS
Sbjct: 1   MKTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSS 60

Query: 66  LKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLG 125
           LKSVSDSAQMLYSY+TVIHGFSTRLTVEEA+LMEK+EGI+AVIPE KYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLG 120

Query: 126 LGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 185
           LGKS SFFPAS KV EVIIG+LDTGVWPELESF+DAGLGP+P SWKGECEVGKNF+SS+C
Sbjct: 121 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNC 180

Query: 186 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 245
           NRKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+G
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 246 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 305
           TARGMAA+ARVATYKVCWLGGCF SDILAA+DK+VEDG N+LS+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAI 300

Query: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 365
           GAFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGE
Sbjct: 301 GAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 366 SLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVV 425
           SLYSGKPLP+SL+PIV AA ASNS+SGS CL+GTLNPAKV GKIVVCDRGGNSRVQKGVV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVV 420

Query: 426 VKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTT 485
           VKEAGG GMILANTE YGEE+LADAHL+P AAVGQK GDAIK+YISSD+NPTA  STGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTT 480

Query: 486 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 545
           RLGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 546 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 605
           IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTP
Sbjct: 541 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTP 600

Query: 606 FDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLE 665
           FDIGAGHVNP AALDPGLVYDTTTDDY  FLCALNY+SLQIKVI+KKDFTC+ +KNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 666 DLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPE 725
           DLNYPSFAVPLETPS RGG + APTT+KYTRTLTNK ASSTYKVS T+KS SVKIVVEPE
Sbjct: 661 DLNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPE 720

Query: 726 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           SLSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Moc08g06250 vs. ExPASy TrEMBL
Match: A0A6J1HBB4 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111462526 PE=3 SV=1)

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 674/773 (87.19%), Postives = 724/773 (93.66%), Query Frame = 0

Query: 4   MKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYD 63
           MK+QTCRVS+ FLLFLI   S S+  A+K++Q+LKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 64  SSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEF 123
           SSLKSVSDSAQ LY Y+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KY+LHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 124 LGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSS 183
           LGL KS SFFPASVKVGEVI+G+LDTGV PELESF+D GLGPVP SWKGECEVGKNFSSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 184 SCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYA 243
           SCNRKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+ 
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFG 240

Query: 244 SGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV 303
           +GTA+GMAA+ARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFT 363
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP Y+TLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 364 GESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKG 423
           G+SLY+GKPL DSL+PIVYAAHASNSTSGS CLT TLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 424 VVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTG 483
           +VVK+AGGAGMILANTETYGEE+LADAHLLPAAAVGQKTGDAIKSYIS++ANPTA  S G
Sbjct: 421 LVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 484 TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVA 543
           +TRLGVQPSP+VAAFSSRGPNLLTP ILKPDLIAPGVNILAGWT   GPTGLD DKR VA
Sbjct: 481 STRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHVA 540

Query: 544 FNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPS 603
           FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GEAIQD+SSGLPS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 604 TPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYK 663
           TPFDIGAGHVNP AALDPGLVYD T +DYF FLCALNY+S QIKVI+KKDFTCS +KNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 664 LEDLNYPSFAVPLETPSVRGGGD-AAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVV 723
           LEDLNYPSFAV LETPS +GG +  APTTVKYTRTLTNK A STYKVS TSKSPSVKI+V
Sbjct: 661 LEDLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 724 EPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           EPESLSFA+ NEQKSYTVTF+AS MPSG+ESFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Moc08g06250 vs. ExPASy TrEMBL
Match: A0A1S3BA54 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103487850 PE=3 SV=1)

HSP 1 Score: 1340.1 bits (3467), Expect = 0.0e+00
Identity = 669/770 (86.88%), Postives = 720/770 (93.51%), Query Frame = 0

Query: 6   MQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 65
           M+TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+T+MPQAFDDHFQWYD+S
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTS 60

Query: 66  LKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLG 125
           LKSVSDSAQMLYSY+ VIHGFST LTVEEA+LMEK+EGI+AV+PE KYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 126 LGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 185
           LGKS SFFPAS KV EVIIGILDTGVWPELESF+D GLGP+P SWKGECEVGKNF+SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 186 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 245
           NRKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+G
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 246 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 305
           TARGMAA+ARVATYKVCWLGGCF SDILAAIDK+VEDG N++S+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 365
           GAFSAAAQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGE
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 366 SLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVV 425
           SLYSGKPLP+SL+PIV AA ASNS+SGS CL+GTLNPAKVAGKIVVCDRGGNSRVQKG+V
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 426 VKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTT 485
           VKEAGG GMILANTE YGEE+LADAHL+P AAVGQ+ GDAIKSYISSD+NPTA  STGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQEAGDAIKSYISSDSNPTATISTGTT 480

Query: 486 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 545
           RLGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 546 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 605
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 606 FDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLE 665
           FDIGAGHVNP AALDPGLVYDTTTDDY  FLCALNY+SLQIKVI+KKDFTC+ +KNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 666 DLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPE 725
           DLNYPSFAVPLETPS + G + APTTVKYTRTLTNK A STYKVS T+K  SVKIVV PE
Sbjct: 661 DLNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKISSVKIVVAPE 720

Query: 726 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           SLSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Moc08g06250 vs. ExPASy TrEMBL
Match: A0A5A7UYG7 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00750 PE=3 SV=1)

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 668/770 (86.75%), Postives = 718/770 (93.25%), Query Frame = 0

Query: 6   MQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 65
           M+TCRVS+ FLLFLI F S S+  A+K+NQ+LKKKTYIIHMD+T+MPQAFDDHFQWYD+ 
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTC 60

Query: 66  LKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLG 125
           LKSVSDSAQMLYSY+ VIHGFST LTVEEA+LMEK+EGI+AV+PE KYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 126 LGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 185
           LGKS SFFPAS KV EVIIGILDTGVWPELESF+D GLGP+P SWKGECEVGKNF+SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 186 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 245
           NRKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+G
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 246 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 305
           TARGMAA+ARVATYKVCWLGGCF SDILAAIDK+VEDG N++S+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 365
           GAFSAAAQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGE
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 366 SLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVV 425
           SLYSGKPLP+SL+PIV AA ASNS+SGS CL+GTLNPAKVAGKIVVCDRGGNSRVQKG+V
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 426 VKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTT 485
           VKEAGG GMILANTE YGEE+LADAHL+P AAVGQK GDAIKSYISSD+NPTA  STGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKSYISSDSNPTATISTGTT 480

Query: 486 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 545
           RLGVQPSPVVAAFSSRGPNLLTP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 546 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 605
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 606 FDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLE 665
           FDIGAGHVNP AALDPGLVYDTTTDDY  FLCALNY+SLQIKVI+KKDFTC+ +KNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 666 DLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPE 725
           DLNYPSFAVPLETPS + G + APTTVKYTRTLTNK   STYKVS T+K  SVKIVV PE
Sbjct: 661 DLNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGVPSTYKVSVTAKISSVKIVVAPE 720

Query: 726 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           SLSF E NEQKSYTVTFIASPMPSG++SFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Moc08g06250 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 994.6 bits (2570), Expect = 4.5e-290
Identity = 494/760 (65.00%), Postives = 602/760 (79.21%), Query Frame = 0

Query: 18  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLY 77
           FL++   + +V +  ++Q     TYI+HM ++ MP +FD H  WYDSSL+S+SDSA++LY
Sbjct: 12  FLLLCLGFCHVSSSSSDQ----GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLY 71

Query: 78  SYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGK-SDSFFPAS 137
           +Y   IHGFSTRLT EEA+ +  + G+++V+PE +YELHTTRTP FLGL + +   FP +
Sbjct: 72  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 131

Query: 138 VKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFA 197
               +V++G+LDTGVWPE +S++D G GP+P+SWKG CE G NF++S CNRKLIGAR+FA
Sbjct: 132 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 191

Query: 198 KGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARV 257
           +GYES  GPIDES+ES+SP+DDDGHGTHTS+TAAGS V GA+L GYASGTARGMA +ARV
Sbjct: 192 RGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARV 251

Query: 258 ATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVF 317
           A YKVCWLGGCF SDILAAIDKA+ D VNVLS+SLGG   DYYRD VAIGAF+A  +G+ 
Sbjct: 252 AVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGIL 311

Query: 318 VSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDS 377
           VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFPA   LGNGK FTG SL+ G+ LPD 
Sbjct: 312 VSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDK 371

Query: 378 LVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMIL 437
           L+P +YA +ASN+T+G+ C+TGTL P KV GKIV+CDRG N+RVQKG VVK AGG GMIL
Sbjct: 372 LLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMIL 431

Query: 438 ANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVA 497
           ANT   GEE +ADAHLLPA  VG+K GD I+ Y+++D NPTA  S   T +GV+PSPVVA
Sbjct: 432 ANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVA 491

Query: 498 AFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPH 557
           AFSSRGPN +TP+ILKPDLIAPGVNILA WTGAAGPTGL  D RRV FNIISGTSMSCPH
Sbjct: 492 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 551

Query: 558 ISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPG 617
           +SGLAAL+K+ HP+WSPAAIRSALMTTAY TYK+G+ + DI++G PSTPFD GAGHV+P 
Sbjct: 552 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPT 611

Query: 618 AALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPL 677
            A +PGL+YD TT+DY  FLCALNYTS QI+ ++++++TC  SK+Y + DLNYPSFAV  
Sbjct: 612 TATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV-- 671

Query: 678 ETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPESLSFAEENEQK 737
              +V G G       KYTRT+T+   + TY V  TS++  VKI VEP  L+F E NE+K
Sbjct: 672 ---NVDGVG-----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 731

Query: 738 SYTVTF-IASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 776
           SYTVTF + S  PSG+ SF  +EWSDGKH+VGSP+A +WT
Sbjct: 732 SYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Moc08g06250 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 769.6 bits (1986), Expect = 2.4e-222
Identity = 417/741 (56.28%), Postives = 513/741 (69.23%), Query Frame = 0

Query: 39  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAE-L 98
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y+T  HGFS  L   EA+ L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 99  MEKREGILAVIPEAKYELHTTRTPEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELES 158
           +     IL +  +  Y LHTTRTPEFLGL               VIIG+LDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 159 FNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARYFAKGYESAF-GPIDESQESKSPK 218
           F+D  +  +P+ WKGECE G +F S  CN+KLIGAR F+KG++ A  G     +ES SP+
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 219 DDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQARVATYKVCWLGGCFGSDILAAI 278
           D DGHGTHTSTTAAGSAV  A+  GYA+GTARGMA +ARVATYKVCW  GCFGSDILAA+
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 279 DKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAP 338
           D+A+ DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 339 WITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCL 398
           W+ TVGAGTLDRDFPA+  LGNGK+ TG SLYSG  +    + +VY  +  NS+S + CL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 399 TGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGGAGMILANTETYGEEELADAHLLPAA 458
            G+L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 459 AVGQKTGDAIKSYISSDANPTAKFSTGTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLI 518
           AVG+KTGD ++ Y+ SD+ PTA      T L V+PSPVVAAFSSRGPN +TP ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 519 APGVNILAGWTGAAGPTGLDGDKRRVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAI 578
            PGVNILAGW+ A GPTGLD D RR  FNI+SGTSMSCPHISGLA L+KAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 579 RSALMTTAYSTYKNGEAIQDISSGLPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFL 638
           +SALMTTAY        + D +    S P+  G+GHV+P  AL PGLVYD +T++Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 639 CALNYTSLQIKVIAKKDFTCSASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTR 698
           C+L+YT   I  I K+     + K      LNYPSF       SV  GG      V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF-------SVLFGG---KRVVRYTR 686

Query: 699 TLTN-KAASSTYKVSETSKSPSVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTE--S 758
            +TN  AASS YKV+  + +PSV I V+P  LSF    E+K YTVTF++    S T    
Sbjct: 687 EVTNVGAASSVYKVT-VNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 759 FARLEWSDGKHIVGSPIAFTW 775
           F  + WS+ +H V SP+AF+W
Sbjct: 747 FGSITWSNPQHEVRSPVAFSW 751

BLAST of Moc08g06250 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 755.0 bits (1948), Expect = 6.0e-218
Identity = 403/775 (52.00%), Postives = 519/775 (66.97%), Query Frame = 0

Query: 17  LFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 76
           LF+I+  +  ++ AE T Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 77  ---DSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLG 136
              ++ ++LY+Y T  HG + +LT EEAE +E+ +G++AVIPE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 137 KSDS--FFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 196
           + +S   +   V   +V++G+LDTG+WPE ESFND G+ PVP +W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 197 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 256
           NRK++GAR F +GYE+A G IDE  E KSP+D DGHGTHT+ T AGS V GANLFG+A G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 257 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 316
           TARGMA +ARVA YKVCW+GGCF SDIL+A+D+AV DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 317 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 376
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFPA + +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 377 SLYSGKPL--PDSLVPIVY-AAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQK 436
           SLY G+ +   +   P+VY   +AS+    S CL G L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 437 GVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFST 496
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  +    TA    
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 497 GTTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRV 556
             TR+G++PSPVVAAFSSRGPN L+  ILKPDL+APGVNILA WTG   P+ L  D RRV
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 557 AFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLP 616
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 617 STPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAK-KDFTCSASKN 676
           S+P+D GAGH++P  A DPGLVYD    +YF FLC  + +  Q+KV  K  + TC  +  
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 677 YKLEDLNYPSF-AVPLETPSVRGGGDAAPTTVKYTRTLTNKAAS-STYKVSETSKSPSVK 736
               +LNYP+  A+  E   V+         +   RT+TN     S+YKVS  S      
Sbjct: 672 KNPGNLNYPAISALFPENTHVK--------AMTLRRTVTNVGPHISSYKVS-VSPFKGAS 731

Query: 737 IVVEPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTW 775
           + V+P++L+F  ++++ SYTVTF  +        F  L W    H V SP+  TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Moc08g06250 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 731.1 bits (1886), Expect = 9.3e-211
Identity = 398/788 (50.51%), Postives = 522/788 (66.24%), Query Frame = 0

Query: 6   MQTCRVSRLFLLF--LIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYD 65
           M    +S +F +F  L+ F+S S   ++        ++YI+H+ R++ P  F  H  W+ 
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGL------ESYIVHVQRSHKPSLFSSHNNWHV 60

Query: 66  SSLKSVSDSAQ---MLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRT 125
           S L+S+  S Q   +LYSYS  +HGFS RL+  +   + +   +++VIP+   E+HTT T
Sbjct: 61  SLLRSLPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHT 120

Query: 126 PEFLGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNF 185
           P FLG  ++   +  S    +VI+G+LDTG+WPE  SF+D+GLGP+P++WKGECE+G +F
Sbjct: 121 PAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 186 SSSSCNRKLIGARYFAKGYESAFGPIDE--SQESKSPKDDDGHGTHTSTTAAGSAVTGAN 245
            +SSCNRKLIGAR F +GY +      +  ++ES+SP+D +GHGTHT++TAAGS V  A+
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 246 LFGYASGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLG--GSSP 305
           L+ YA GTA GMA++AR+A YK+CW GGC+ SDILAA+D+AV DGV+V+SLS+G  GS+P
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 306 DYYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITL 365
           +Y+ D++AIGAF A   G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 366 GNGKKFTGESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGG 425
           G+GK FTG SLY+G+ LPDS + +VY    S       C  G LN + V GKIV+CDRGG
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGG 420

Query: 426 NSRVQKGVVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANP 485
           N+RV+KG  VK AGGAGMILANT   GEE  AD+HL+PA  VG K GD I+ YI +  +P
Sbjct: 421 NARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSP 480

Query: 486 TAKFSTGTTRLGVQ-PSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGL 545
           TAK S   T +G   PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGWTG  GPT L
Sbjct: 481 TAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDL 540

Query: 546 DGDKRRVAFNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQ 605
           D D RRV FNIISGTSMSCPH+SGLAAL++ AHPDWSPAAI+SAL+TTAY    +GE I+
Sbjct: 541 DIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIE 600

Query: 606 DISSGLPSTPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDF- 665
           D+++G  S  F  GAGHV+P  AL+PGLVYD    +Y  FLCA+ Y    I V  +    
Sbjct: 601 DLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTL 660

Query: 666 --TCSASKNYKLEDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSET 725
              C  SK     DLNYPSF+V   +             VKY R + N  ++        
Sbjct: 661 YDACETSKLRTAGDLNYPSFSVVFASTG---------EVVKYKRVVKNVGSNVDAVYEVG 720

Query: 726 SKSP-SVKIVVEPESLSFAEENEQKSYTVTFIASPMPSGTES-----FARLEWSDGKHIV 775
            KSP +V+I V P  L+F++E     Y VTF +  +  G  S     F  +EW+DG+H+V
Sbjct: 721 VKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVV 769

BLAST of Moc08g06250 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 692.6 bits (1786), Expect = 3.7e-199
Identity = 377/774 (48.71%), Postives = 497/774 (64.21%), Query Frame = 0

Query: 18  FLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDS-AQML 77
           F   F+  +      +       TYI+H+D    P  F  HF WY SSL S++ S   ++
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSII 63

Query: 78  YSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLGLGKSD--SFFP 137
           ++Y TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  +D      
Sbjct: 64  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 123

Query: 138 ASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSCNRKLIGARY 197
            S    +++IG++DTGVWPE  SF+D GLGPVP  WKG+C   ++F  S+CNRKL+GAR+
Sbjct: 124 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 183

Query: 198 FAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASGTARGMAAQA 257
           F  GYE+  G ++E+ E +SP+D DGHGTHT++ +AG  V  A+  GYA G A GMA +A
Sbjct: 184 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 243

Query: 258 RVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAIGAFSAAAQG 317
           R+A YKVCW  GC+ SDILAA D AV DGV+V+SLS+GG    YY D +AIGAF A  +G
Sbjct: 244 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRG 303

Query: 318 VFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGESLYSGKPL- 377
           +FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFPA + LGNGK  +G S+Y G  L 
Sbjct: 304 IFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLD 363

Query: 378 PDSLVPIVYAAH--ASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVVVKEAGG 437
           P  + P+VY       +  S S CL G+L+P  V GKIV+CDRG NSR  KG +V++ GG
Sbjct: 364 PGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGG 423

Query: 438 AGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYIS------SDANPTAKFSTGTT 497
            GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      S  +PTA      T
Sbjct: 424 LGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGT 483

Query: 498 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 557
           RLG++P+PVVA+FS+RGPN  TP ILKPD+IAPG+NILA W    GP+G+  D RR  FN
Sbjct: 484 RLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFN 543

Query: 558 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 617
           I+SGTSM+CPH+SGLAAL+KAAHPDWSPAAIRSAL+TTAY+   +GE + D S+G  S+ 
Sbjct: 544 ILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSV 603

Query: 618 FDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNY-KL 677
            D G+GHV+P  A+DPGLVYD T+ DY  FLC  NYT   I  I ++   C  ++    +
Sbjct: 604 MDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV 663

Query: 678 EDLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEP 737
            +LNYPSF+V  +       G++  +T  + RT+TN   S +    +        + VEP
Sbjct: 664 GNLNYPSFSVVFQQ-----YGESKMST-HFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEP 723

Query: 738 ESLSFAEENEQKSY-----TVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFT 774
           E LSF    ++ S+     T     SP  +  E+   + WSDGK  V SP+  T
Sbjct: 724 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET-GHIVWSDGKRNVTSPLVVT 770