Moc08g05650 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g05650
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionmitogen-activated protein kinase kinase kinase 17-like
Locationchr8: 4133326 .. 4134284 (+)
RNA-Seq ExpressionMoc08g05650
SyntenyMoc08g05650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCAATAAGGTCCTTCAAAGAATATGATCTCTCAGCCTTCGATTGGGACAAAACTCCAGTTTTAGGCAAAGGTTCTCATGGCAAAGTCTTCCTAGGAGAACTCGATCTCGGCAGCGTCGCCATCAAAGTCGCTCCATGTCGAGCAGCTTCTTCACTCGTCAAAGAGAAAGCGATTCTCAAACAGTTCATCGGATGCTCAAAAATCGTTCAGTACATTGGCGACACCGTTACTAAGAAAGGAGACGACGTTGTTTACAGTCTGATATTGGAGTATGCTACTGGAGGTACTTTGGGTGACATTATCAGAAAACAGGAGAAAATTTCAGAGGAAGATGCCAAAAAGTACCTTCGAATGATACTTCAAGGGCTTTCTTGCATTCACGCTAAAGGGTTTGTGCATGCTGATCTCAAATCGGACAATATTCTTGCGTTCCGCTCAAAGAGTGGAAGAATGAAGCTGAAAATAGCAGATTTTGGACTTTCTAGGATGTATGATGAGAAAGAAGAGAAAAGTTTATGTTGGAATTATTACAAGATGAGCTTTGTAGGGACAGCAAAATACATGTCTCCAGAATCTGTCTTTGACATCGTTAAACCCGCACTCGATATTTGGTCTCTTGGTTGCATTTTTGTTAACATGATGACCGGGAGACACGTTTGGAATGATTGCAAAGATAATAAAGAGCTGATTTCGAAGCTTATATATGAAAGAGAAATACCAACAATACCAAAAGAATTATCCGAGCTAGGAAAGGATTTTCTCGAAAAGTGCTTTGAGAGAAATTACGAAAAAAGATGGTCAGCAGATATGCTACTTCGACATCCTTATTTGGTAGAAGGAAAAAAAGTTACGAACAAAATCAGCTGTAGAAGTCTTTCTATTTTGCTTTGTACAGGGGCCTTAGTCATTCTTCTTTTAGATTGCTTTCTTATTTTTCAGTTGTTTTCTCTTAA

mRNA sequence

ATGGCACCAATAAGGTCCTTCAAAGAATATGATCTCTCAGCCTTCGATTGGGACAAAACTCCAGTTTTAGGCAAAGGTTCTCATGGCAAAGTCTTCCTAGGAGAACTCGATCTCGGCAGCGTCGCCATCAAAGTCGCTCCATGTCGAGCAGCTTCTTCACTCGTCAAAGAGAAAGCGATTCTCAAACAGTTCATCGGATGCTCAAAAATCGTTCAGTACATTGGCGACACCGTTACTAAGAAAGGAGACGACGTTGTTTACAGTCTGATATTGGAGTATGCTACTGGAGGTACTTTGGGTGACATTATCAGAAAACAGGAGAAAATTTCAGAGGAAGATGCCAAAAAGTACCTTCGAATGATACTTCAAGGGCTTTCTTGCATTCACGCTAAAGGGTTTGTGCATGCTGATCTCAAATCGGACAATATTCTTGCGTTCCGCTCAAAGAGTGGAAGAATGAAGCTGAAAATAGCAGATTTTGGACTTTCTAGGATGTATGATGAGAAAGAAGAGAAAAGTTTATGTTGGAATTATTACAAGATGAGCTTTGTAGGGACAGCAAAATACATGTCTCCAGAATCTGTCTTTGACATCGTTAAACCCGCACTCGATATTTGGTCTCTTGGTTGCATTTTTGTTAACATGATGACCGGGAGACACGTTTGGAATGATTGCAAAGATAATAAAGAGCTGATTTCGAAGCTTATATATGAAAGAGAAATACCAACAATACCAAAAGAATTATCCGAGCTAGGAAAGGATTTTCTCGAAAAGTGCTTTGAGAGAAATTACGAAAAAAGATGGGGCCTTAGTCATTCTTCTTTTAGATTGCTTTCTTATTTTTCAGTTGTTTTCTCTTAA

Coding sequence (CDS)

ATGGCACCAATAAGGTCCTTCAAAGAATATGATCTCTCAGCCTTCGATTGGGACAAAACTCCAGTTTTAGGCAAAGGTTCTCATGGCAAAGTCTTCCTAGGAGAACTCGATCTCGGCAGCGTCGCCATCAAAGTCGCTCCATGTCGAGCAGCTTCTTCACTCGTCAAAGAGAAAGCGATTCTCAAACAGTTCATCGGATGCTCAAAAATCGTTCAGTACATTGGCGACACCGTTACTAAGAAAGGAGACGACGTTGTTTACAGTCTGATATTGGAGTATGCTACTGGAGGTACTTTGGGTGACATTATCAGAAAACAGGAGAAAATTTCAGAGGAAGATGCCAAAAAGTACCTTCGAATGATACTTCAAGGGCTTTCTTGCATTCACGCTAAAGGGTTTGTGCATGCTGATCTCAAATCGGACAATATTCTTGCGTTCCGCTCAAAGAGTGGAAGAATGAAGCTGAAAATAGCAGATTTTGGACTTTCTAGGATGTATGATGAGAAAGAAGAGAAAAGTTTATGTTGGAATTATTACAAGATGAGCTTTGTAGGGACAGCAAAATACATGTCTCCAGAATCTGTCTTTGACATCGTTAAACCCGCACTCGATATTTGGTCTCTTGGTTGCATTTTTGTTAACATGATGACCGGGAGACACGTTTGGAATGATTGCAAAGATAATAAAGAGCTGATTTCGAAGCTTATATATGAAAGAGAAATACCAACAATACCAAAAGAATTATCCGAGCTAGGAAAGGATTTTCTCGAAAAGTGCTTTGAGAGAAATTACGAAAAAAGATGGGGCCTTAGTCATTCTTCTTTTAGATTGCTTTCTTATTTTTCAGTTGTTTTCTCTTAA

Protein sequence

MAPIRSFKEYDLSAFDWDKTPVLGKGSHGKVFLGELDLGSVAIKVAPCRAASSLVKEKAILKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTIPKELSELGKDFLEKCFERNYEKRWGLSHSSFRLLSYFSVVFS
Homology
BLAST of Moc08g05650 vs. NCBI nr
Match: XP_022131646.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 549.7 bits (1415), Expect = 1.5e-152
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MAPIRSFKEYDLSAFDWDKTPVLGKGSHGKVFLGELDLGSVAIKVAPCRAASSLVKEKAI 60
           MAPIRSFKEYDLSAFDWDKTPVLGKGSHGKVFLGELDLGSVAIKVAPCRAASSLVKEKAI
Sbjct: 1   MAPIRSFKEYDLSAFDWDKTPVLGKGSHGKVFLGELDLGSVAIKVAPCRAASSLVKEKAI 60

Query: 61  LKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRM 120
           LKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRM
Sbjct: 61  LKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRM 120

Query: 121 ILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYK 180
           ILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYK
Sbjct: 121 ILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYK 180

Query: 181 MSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYERE 240
           MSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYERE
Sbjct: 181 MSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYERE 240

Query: 241 IPTIPKELSELGKDFLEKCFERNYEKRW 269
           IPTIPKELSELGKDFLEKCFERNYEKRW
Sbjct: 241 IPTIPKELSELGKDFLEKCFERNYEKRW 268

BLAST of Moc08g05650 vs. NCBI nr
Match: XP_022131647.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 371.3 bits (952), Expect = 7.4e-99
Identity = 186/276 (67.39%), Postives = 222/276 (80.43%), Query Frame = 0

Query: 1   MAPIRSF-KEYDLSAFDWDKTPVLGKGSHGKVFLG---ELDLGSVAIKVAPC-RAASSLV 60
           MAP   F +E D+S FDW+K   LGKGS+GKVFLG   + DLG VAIKVAP  +  SSL+
Sbjct: 1   MAPTIPFTEEDDISVFDWNKAQFLGKGSYGKVFLGTPTKPDLGFVAIKVAPLWQKTSSLI 60

Query: 61  KEKAILKQFIGCSKIVQYIGDTVTKKGDD-VVYSLILEYATGGTLGDIIRKQEKISEEDA 120
           KEK +L+QFIGC +IVQYIGD VT K DD +VY+LILEYA GGTL DII+KQ+K+ EEDA
Sbjct: 61  KEKVVLEQFIGCPEIVQYIGDAVTIKRDDHLVYNLILEYAAGGTLADIIKKQKKLQEEDA 120

Query: 121 KKYLRMILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSL 180
           K+YLRMIL+GLSCIHAK FVHADLK  NILAF  K GRM+LKIADFGL+R+Y +KEE+  
Sbjct: 121 KQYLRMILRGLSCIHAKSFVHADLKPTNILAFPLKDGRMQLKIADFGLARIYYKKEERDF 180

Query: 181 CWNYYKMSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISK 240
            W   KM FVGT +YMSPESV   + P+LDIWSLGCIF+NM+TG  VWNDC   ++LI+K
Sbjct: 181 YWKNNKMKFVGTTEYMSPESVVGNIDPSLDIWSLGCIFLNMITGECVWNDCLTYEQLITK 240

Query: 241 LIYEREIPTIPKELSELGKDFLEKCFERNYEKRWGL 271
           L+YE E PTIP+ELS+LGKDFL+KCFERNYE+RW +
Sbjct: 241 LVYEGETPTIPEELSDLGKDFLQKCFERNYEERWSV 276

BLAST of Moc08g05650 vs. NCBI nr
Match: XP_038885921.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 278.9 bits (712), Expect = 5.0e-71
Identity = 143/263 (54.37%), Postives = 191/263 (72.62%), Query Frame = 0

Query: 12  LSAFDWDKTPVLGKGSHGKVFLGELD---LGSVAIKVAPCRAASSLVKEKAILKQFIGCS 71
           +S  +WDK  VLG+GS G V+LG  +   L +VAIK AP + A SL++EK +L++FIGC 
Sbjct: 10  ISISNWDKGSVLGRGSFGHVYLGVPNKPYLANVAIKSAPYQTACSLIREKVVLQKFIGCP 69

Query: 72  KIVQYIGDTVTKKGDD-VVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLSC 131
           +IVQYIG+ +T   DD +VY LILEYA  G+L  +I+ + ++ E + K YLRMILQGLSC
Sbjct: 70  EIVQYIGEALTVNTDDNIVYDLILEYAASGSLAHLIKTRGRLLEREVKVYLRMILQGLSC 129

Query: 132 IHAKGFVHADLKSDNILAFR-SKSGRMKLKIADFGLS-RMYDEKEEKSLCWNYYKMSFVG 191
           IHA+G+VH DLK +NIL F      +MKLKIADF LS R + +K E+S      K+ F G
Sbjct: 130 IHARGYVHCDLKPENILVFPIGDRKKMKLKIADFRLSERYFGKKLEES-----GKLKFRG 189

Query: 192 TAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTIP 251
           T KYMSPES+   +KP+LDIWSLGC+ VNM++G+ VWNDC   +E++ KL++E + P IP
Sbjct: 190 TPKYMSPESLIGKIKPSLDIWSLGCVLVNMISGKCVWNDCTTREEVLLKLMFEMKTPNIP 249

Query: 252 KELSELGKDFLEKCFERNYEKRW 269
           +ELS  GKDFL+KCFERN ++RW
Sbjct: 250 EELSFQGKDFLQKCFERNPKERW 267

BLAST of Moc08g05650 vs. NCBI nr
Match: XP_022150204.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 273.5 bits (698), Expect = 2.1e-69
Identity = 142/259 (54.83%), Postives = 180/259 (69.50%), Query Frame = 0

Query: 16  DWDKTPVLGKGSHGKVFLGELDLGSV---AIKVAPCRAASSLVKEKAILKQFIGCSKIVQ 75
           +W +   LGKGS+GKVFL +    S+   A+KVA  +  SSL+ EK ILK F+G  +IVQ
Sbjct: 3   NWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQ 62

Query: 76  YIGDTVTKKG---DDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLSCIH 135
             G  +  +G   D   YSL+LEYA GGTL D+IRKQ+K+SE   K+YL+MIL+GLSCIH
Sbjct: 63  CFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSCIH 122

Query: 136 AKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKY 195
             GFVH DLK  NILAF    G+MKLKIADFGLSR  ++K E +     +K+ F GT  Y
Sbjct: 123 KLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECN-----FKLKFRGTPMY 182

Query: 196 MSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTIPKELS 255
           MSPES++  V   LDIWSLGCI V M+TG+H W+DC D ++L+ KL    E P IP+ELS
Sbjct: 183 MSPESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELS 242

Query: 256 ELGKDFLEKCFERNYEKRW 269
           + GKDFLEKCF+RN ++RW
Sbjct: 243 KEGKDFLEKCFDRNPKRRW 256

BLAST of Moc08g05650 vs. NCBI nr
Match: XP_022154080.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 261.9 bits (668), Expect = 6.3e-66
Identity = 137/257 (53.31%), Postives = 182/257 (70.82%), Query Frame = 0

Query: 17  WDKTPVLGKGSHGKVFLGELDLGS---VAIKVAPCRAASSLVKEKAILKQFIGCSKIVQY 76
           W K   LG+GS+GKVFL +  +G    VA+KVAP   +SSL  EK IL+   GCS+IVQ 
Sbjct: 4   WTKVKELGRGSYGKVFLAKSTIGPIDYVAVKVAPVERSSSLELEKDILQHLRGCSEIVQC 63

Query: 77  IGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLSCIHAKGF 136
           IG  +T     V Y LILEYA GGTL ++IRKQ+K++E   K+YLRMIL+GLSCIH +GF
Sbjct: 64  IGSEMT----SVCYYLILEYAGGGTLAELIRKQKKLTESKVKEYLRMILRGLSCIHRRGF 123

Query: 137 VHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKYMSPE 196
           VHADLK  NILAF   +G+MKLKIADFGLS+ +   ++++    + +  F GTA+YMSPE
Sbjct: 124 VHADLKPANILAFPRPNGKMKLKIADFGLSKRWVVGQDEAA--EHSEPRFRGTARYMSPE 183

Query: 197 S-VFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPT-IPKELSEL 256
           S +F  + P LDIWSLGCIF+ M+TG  VWN C+   +L+ KL+ E E+PT + K+LS+ 
Sbjct: 184 SIIFGKISPPLDIWSLGCIFIEMITGSPVWNSCRTYDQLLKKLVNETELPTDVLKKLSKE 243

Query: 257 GKDFLEKCFERNYEKRW 269
           G+DFL  C +R+ ++RW
Sbjct: 244 GRDFLASCLDRDSKRRW 254

BLAST of Moc08g05650 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.1e-33
Identity = 93/265 (35.09%), Postives = 138/265 (52.08%), Query Frame = 0

Query: 16  DWDKTPVLGKGSHGKVFLGELDLGS------VAIKVAPCRAASSLVKEKAILKQFIGCSK 75
           +W +   +G G+   V        S      +A+K      A+SL  EK++L     C +
Sbjct: 2   EWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPE 61

Query: 76  IVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRK--QEKISEEDAKKYLRMILQGLSC 135
           I++  G+  T +  + +++L+LEYA+ G+L   ++K   E + E   +++   +L+GL  
Sbjct: 62  IIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRH 121

Query: 136 IHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTA 195
           IHAKGF H D+K  NIL F   S    +KIADFGL+   D      L      +   GT 
Sbjct: 122 IHAKGFAHCDIKLANILLFNDGS----VKIADFGLAMRVD----GDLTALRKSVEIRGTP 181

Query: 196 KYMSPESVFD-IVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLI---YEREIPT 255
            YM+PE V D     A D+W+LGC  V M +G+  W+  K+    +S LI      E+P 
Sbjct: 182 LYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWS-VKEGSHFMSLLIRIGVGDELPK 241

Query: 256 IPKELSELGKDFLEKCFERNYEKRW 269
           IP+ LSE GKDFL KCF ++  KRW
Sbjct: 242 IPEMLSEEGKDFLSKCFVKDPAKRW 257

BLAST of Moc08g05650 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 6.4e-29
Identity = 84/261 (32.18%), Postives = 132/261 (50.57%), Query Frame = 0

Query: 16  DWDKTPVLGKGSHGKVF--LGELDLGSVAIKVAPCRAASSLVKEKAILKQFIGCSKIVQY 75
           +W +  +LG+GS   V+   G      +A+K +    +  L +E  IL   +    ++ Y
Sbjct: 2   EWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSS-LSSPYVIGY 61

Query: 76  IGDTVTKKGDDVV-YSLILEYATGGTLGDIIRKQ-EKISEEDAKKYLRMILQGLSCIHAK 135
            G    ++ + VV Y+L++EYA  GTL D   K   ++ E    KY R IL+GL  IH+K
Sbjct: 62  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 121

Query: 136 GFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKYMS 195
           G VH D+K  N++     S + + KIADFG ++  D           ++   +GT  +M+
Sbjct: 122 GIVHCDVKGSNVVI----SEKGEAKIADFGCAKRVDP---------VFESPVMGTPAFMA 181

Query: 196 PE-SVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLI---YEREIPTIPKE 255
           PE +  +      DIW++GC  + M+TG   W      ++ +S L    Y  E P +P  
Sbjct: 182 PEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCL 241

Query: 256 LSELGKDFLEKCFERNYEKRW 269
           L+E  KDFLEKC +R   +RW
Sbjct: 242 LAEEAKDFLEKCLKREANERW 248

BLAST of Moc08g05650 vs. ExPASy Swiss-Prot
Match: Q01389 (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BCK1 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.3e-28
Identity = 93/279 (33.33%), Postives = 142/279 (50.90%), Query Frame = 0

Query: 15   FDWDKTPVLGKGSHGKVFL------GELDLGSVAIKVAPCRAASS-----------LVKE 74
            F W K  ++GKGS G V+L      GE+    +A+K       SS           L  E
Sbjct: 1173 FAWMKGEMIGKGSFGAVYLCLNVTTGEM----MAVKQVEVPKYSSQNEAILSTVEALRSE 1232

Query: 75   KAILKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKY 134
             + LK  +    IVQY+G     +  + +YSL LEY  GG++G +IR   +  E   K  
Sbjct: 1233 VSTLKD-LDHLNIVQYLG----FENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHL 1292

Query: 135  LRMILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWN 194
               +L+GL+ +H+KG +H D+K+DN+L  +        KI+DFG+SR   +    S    
Sbjct: 1293 TTQVLKGLAYLHSKGILHRDMKADNLLLDQDGI----CKISDFGISRKSKDIYSNS---- 1352

Query: 195  YYKMSFVGTAKYMSPESV--FDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELIS-- 254
               M+  GT  +M+PE V         +DIWSLGCI + M  G+  W+    N E+++  
Sbjct: 1353 --DMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKRPWS----NLEVVAAM 1412

Query: 255  -KLIYEREIPTIPKE----LSELGKDFLEKCFERNYEKR 268
             K+   +  P IP++    +S++G++FL+ CFE N EKR
Sbjct: 1413 FKIGKSKSAPPIPEDTLPLISQIGRNFLDACFEINPEKR 1428

BLAST of Moc08g05650 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.6e-27
Identity = 88/263 (33.46%), Postives = 136/263 (51.71%), Query Frame = 0

Query: 17  WDKTPVLGKGSHGKVFLG-ELDLGSVAI--KVAPC-------RAASSLVKEKAILKQFIG 76
           W K  +LG GS G V+LG   + G +    +V  C        +A  L +E ++L + + 
Sbjct: 400 WKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSR-LR 459

Query: 77  CSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLS 136
              IVQY G       DD +Y + LEY +GG++  ++++  +  E   + Y + IL GL+
Sbjct: 460 HQNIVQYYGSETV---DDKLY-IYLEYVSGGSIYKLLQEYGQFGENAIRNYTQQILSGLA 519

Query: 137 CIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGT 196
            +HAK  VH D+K  NIL      GR  +K+ADFG++        K +      +SF G+
Sbjct: 520 YLHAKNTVHRDIKGANILV--DPHGR--VKVADFGMA--------KHITAQSGPLSFKGS 579

Query: 197 AKYMSPESVFDI--VKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTI 256
             +M+PE + +      A+DIWSLGC  + M T +  W+   +    + K+   +E+P I
Sbjct: 580 PYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQ-YEGVPAMFKIGNSKELPDI 639

Query: 257 PKELSELGKDFLEKCFERNYEKR 268
           P  LSE GKDF+ KC +RN   R
Sbjct: 640 PDHLSEEGKDFVRKCLQRNPANR 644

BLAST of Moc08g05650 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 3.0e-26
Identity = 83/263 (31.56%), Postives = 132/263 (50.19%), Query Frame = 0

Query: 17  WDKTPVLGKGSHGKVFLG-ELDLGSV-AIKVAPCRAASSLVKEKAILKQF---------I 76
           W K  +LG+G +G V+LG   D G + A+K       +S  K K ++  F         +
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSL 229

Query: 77  GCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGL 136
               IV+Y+G ++    D    S+ LEY  GG++  ++ K    SE   K Y + ILQGL
Sbjct: 230 RHDNIVRYLGTSL----DQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGL 289

Query: 137 SCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVG 196
           S +HA   +H D+K  NIL       +  +K++DFG S+ +      S   + +K S  G
Sbjct: 290 SFLHANSIIHRDIKGANILI----DTKGIVKLSDFGCSKSF------SGIVSQFK-SMQG 349

Query: 197 TAKYMSPESVFDIVK-PALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTI 256
           T  +M+PE +       + DIWSLGC+ V M T +  W++  +   ++  +     IP I
Sbjct: 350 TPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNI 409

Query: 257 PKELSELGKDFLEKCFERNYEKR 268
           P  +S+   DFL  CF+R+ ++R
Sbjct: 410 PSHMSQEAFDFLNLCFKRDPKER 417

BLAST of Moc08g05650 vs. ExPASy TrEMBL
Match: A0A6J1BQ31 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111004774 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.4e-153
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MAPIRSFKEYDLSAFDWDKTPVLGKGSHGKVFLGELDLGSVAIKVAPCRAASSLVKEKAI 60
           MAPIRSFKEYDLSAFDWDKTPVLGKGSHGKVFLGELDLGSVAIKVAPCRAASSLVKEKAI
Sbjct: 1   MAPIRSFKEYDLSAFDWDKTPVLGKGSHGKVFLGELDLGSVAIKVAPCRAASSLVKEKAI 60

Query: 61  LKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRM 120
           LKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRM
Sbjct: 61  LKQFIGCSKIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRM 120

Query: 121 ILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYK 180
           ILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYK
Sbjct: 121 ILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYK 180

Query: 181 MSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYERE 240
           MSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYERE
Sbjct: 181 MSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYERE 240

Query: 241 IPTIPKELSELGKDFLEKCFERNYEKRW 269
           IPTIPKELSELGKDFLEKCFERNYEKRW
Sbjct: 241 IPTIPKELSELGKDFLEKCFERNYEKRW 268

BLAST of Moc08g05650 vs. ExPASy TrEMBL
Match: A0A6J1BRL7 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111004775 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.6e-99
Identity = 186/276 (67.39%), Postives = 222/276 (80.43%), Query Frame = 0

Query: 1   MAPIRSF-KEYDLSAFDWDKTPVLGKGSHGKVFLG---ELDLGSVAIKVAPC-RAASSLV 60
           MAP   F +E D+S FDW+K   LGKGS+GKVFLG   + DLG VAIKVAP  +  SSL+
Sbjct: 1   MAPTIPFTEEDDISVFDWNKAQFLGKGSYGKVFLGTPTKPDLGFVAIKVAPLWQKTSSLI 60

Query: 61  KEKAILKQFIGCSKIVQYIGDTVTKKGDD-VVYSLILEYATGGTLGDIIRKQEKISEEDA 120
           KEK +L+QFIGC +IVQYIGD VT K DD +VY+LILEYA GGTL DII+KQ+K+ EEDA
Sbjct: 61  KEKVVLEQFIGCPEIVQYIGDAVTIKRDDHLVYNLILEYAAGGTLADIIKKQKKLQEEDA 120

Query: 121 KKYLRMILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSL 180
           K+YLRMIL+GLSCIHAK FVHADLK  NILAF  K GRM+LKIADFGL+R+Y +KEE+  
Sbjct: 121 KQYLRMILRGLSCIHAKSFVHADLKPTNILAFPLKDGRMQLKIADFGLARIYYKKEERDF 180

Query: 181 CWNYYKMSFVGTAKYMSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISK 240
            W   KM FVGT +YMSPESV   + P+LDIWSLGCIF+NM+TG  VWNDC   ++LI+K
Sbjct: 181 YWKNNKMKFVGTTEYMSPESVVGNIDPSLDIWSLGCIFLNMITGECVWNDCLTYEQLITK 240

Query: 241 LIYEREIPTIPKELSELGKDFLEKCFERNYEKRWGL 271
           L+YE E PTIP+ELS+LGKDFL+KCFERNYE+RW +
Sbjct: 241 LVYEGETPTIPEELSDLGKDFLQKCFERNYEERWSV 276

BLAST of Moc08g05650 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 1.0e-69
Identity = 142/259 (54.83%), Postives = 180/259 (69.50%), Query Frame = 0

Query: 16  DWDKTPVLGKGSHGKVFLGELDLGSV---AIKVAPCRAASSLVKEKAILKQFIGCSKIVQ 75
           +W +   LGKGS+GKVFL +    S+   A+KVA  +  SSL+ EK ILK F+G  +IVQ
Sbjct: 3   NWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQ 62

Query: 76  YIGDTVTKKG---DDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLSCIH 135
             G  +  +G   D   YSL+LEYA GGTL D+IRKQ+K+SE   K+YL+MIL+GLSCIH
Sbjct: 63  CFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSCIH 122

Query: 136 AKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKY 195
             GFVH DLK  NILAF    G+MKLKIADFGLSR  ++K E +     +K+ F GT  Y
Sbjct: 123 KLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECN-----FKLKFRGTPMY 182

Query: 196 MSPESVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTIPKELS 255
           MSPES++  V   LDIWSLGCI V M+TG+H W+DC D ++L+ KL    E P IP+ELS
Sbjct: 183 MSPESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELS 242

Query: 256 ELGKDFLEKCFERNYEKRW 269
           + GKDFLEKCF+RN ++RW
Sbjct: 243 KEGKDFLEKCFDRNPKRRW 256

BLAST of Moc08g05650 vs. ExPASy TrEMBL
Match: A0A6J1DIL5 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111021423 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 3.1e-66
Identity = 137/257 (53.31%), Postives = 182/257 (70.82%), Query Frame = 0

Query: 17  WDKTPVLGKGSHGKVFLGELDLGS---VAIKVAPCRAASSLVKEKAILKQFIGCSKIVQY 76
           W K   LG+GS+GKVFL +  +G    VA+KVAP   +SSL  EK IL+   GCS+IVQ 
Sbjct: 4   WTKVKELGRGSYGKVFLAKSTIGPIDYVAVKVAPVERSSSLELEKDILQHLRGCSEIVQC 63

Query: 77  IGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLSCIHAKGF 136
           IG  +T     V Y LILEYA GGTL ++IRKQ+K++E   K+YLRMIL+GLSCIH +GF
Sbjct: 64  IGSEMT----SVCYYLILEYAGGGTLAELIRKQKKLTESKVKEYLRMILRGLSCIHRRGF 123

Query: 137 VHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKYMSPE 196
           VHADLK  NILAF   +G+MKLKIADFGLS+ +   ++++    + +  F GTA+YMSPE
Sbjct: 124 VHADLKPANILAFPRPNGKMKLKIADFGLSKRWVVGQDEAA--EHSEPRFRGTARYMSPE 183

Query: 197 S-VFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPT-IPKELSEL 256
           S +F  + P LDIWSLGCIF+ M+TG  VWN C+   +L+ KL+ E E+PT + K+LS+ 
Sbjct: 184 SIIFGKISPPLDIWSLGCIFIEMITGSPVWNSCRTYDQLLKKLVNETELPTDVLKKLSKE 243

Query: 257 GKDFLEKCFERNYEKRW 269
           G+DFL  C +R+ ++RW
Sbjct: 244 GRDFLASCLDRDSKRRW 254

BLAST of Moc08g05650 vs. ExPASy TrEMBL
Match: A0A6J1DHA9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111020049 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 2.6e-65
Identity = 133/260 (51.15%), Postives = 175/260 (67.31%), Query Frame = 0

Query: 17  WDKTPVLGKGSHGKVFLGELDLGS---VAIKVAPCRAASSLVKEKAILKQFIGCSKIVQY 76
           W +  +LG+GS GKVFL +  +G    VA+K A    +SSL+ EK IL+ F+GCS+I+Q 
Sbjct: 15  WTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSEIIQC 74

Query: 77  IGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQEKISEEDAKKYLRMILQGLSCIHAKGF 136
           IGD +T +   + Y+LILEYA GGTL ++I+KQ+ + E + K+YL+MIL+GLSCIH KGF
Sbjct: 75  IGDEITLEESSLCYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLSCIHDKGF 134

Query: 137 VHADLKSDNILAFRSKSGRMKLKIADFGLSRM---YDEKEEKSLCWNYYKMSFVGTAKYM 196
           VH DLK  NILAF    G+MKLKIADFGLS+     D +          K  F GT  YM
Sbjct: 135 VHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKFKGTPIYM 194

Query: 197 SPESV-FDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPT-IPKEL 256
           SPESV F  + P  DIWSLGCI V M+TG+ VW  C+ + + I KL+YE E+P  IP+EL
Sbjct: 195 SPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSGQFIKKLVYEMELPADIPEEL 254

Query: 257 SELGKDFLEKCFERNYEKRW 269
           S  G+DFL  CF+ + ++RW
Sbjct: 255 SREGRDFLASCFDHDPKRRW 274

BLAST of Moc08g05650 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 158.3 bits (399), Expect = 9.2e-39
Identity = 93/257 (36.19%), Postives = 144/257 (56.03%), Query Frame = 0

Query: 16  DWDKTPVLGKGSHGKVFLGELDLGS-VAIKVAPCRAASSLVKEKAILKQFIGCSKIVQYI 75
           +W + P++G+GS   V LG  + G   A+K A   +++ L +E++IL + +    IV+YI
Sbjct: 5   NWIRGPIIGRGSTATVSLGITNSGDFFAVKSAEFSSSAFLQREQSILSK-LSSPYIVKYI 64

Query: 76  GDTVTKKGDDVVYSLILEYATGGTLGDIIRKQ-EKISEEDAKKYLRMILQGLSCIHAKGF 135
           G  VTK+ D ++Y+L++EY +GG+L D+I+    K+ E   + Y R IL+GL  +H +G 
Sbjct: 65  GSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGI 124

Query: 136 VHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKYMSPE 195
           VH D+KS N++      G    KI D G ++  +E E          + F GT  +MSPE
Sbjct: 125 VHCDVKSQNVMI-----GGEIAKIVDLGCAKTVEENE---------NLEFSGTPAFMSPE 184

Query: 196 SVFDIVK--PALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTIPKELSEL 255
                 +  PA D+W+LGC  + M TG   W +  D    I K+ +  E P IP  LSE 
Sbjct: 185 VARGEEQSFPA-DVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEK 244

Query: 256 GKDFLEKCFERNYEKRW 269
           G+DFL KC  ++ ++RW
Sbjct: 245 GQDFLRKCLRKDPKQRW 245

BLAST of Moc08g05650 vs. TAIR 10
Match: AT2G42550.1 (Protein kinase superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 3.5e-38
Identity = 102/255 (40.00%), Postives = 144/255 (56.47%), Query Frame = 0

Query: 42  AIKVAPCRAASSLVKEKAILKQFIGCSKIVQYIGDTVTKKGDDV----VYSLILEYATGG 101
           A+K A C   +SL +E  IL +  GC +IVQ  G+   ++  DV    VY +++EYA  G
Sbjct: 37  AVKTAECEDYNSLEREIQILSKLEGCRRIVQCYGNYTLEEDFDVGGFRVYKMVMEYAAAG 96

Query: 102 TLGDII--RKQEKISEEDAKKYLRMILQGLSCIHAKGFVHADLKSDNILAFRSKSGRMKL 161
           +L   +   K  K+ E   K + RMILQGL  +H  G+VH DLK DN+L F  +    +L
Sbjct: 97  SLFSFMDSYKDRKLPETMIKDFTRMILQGLVSVHRLGYVHCDLKPDNLLVFPCRQS-YEL 156

Query: 162 KIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKYMSPESV-FDIVKPALDIWSLGCIFVN 221
           KI+DFG SR   E  +   CW+   + FVGT  YMSPESV   + + ALD+WSLGCI + 
Sbjct: 157 KISDFGSSRKVGEYSD---CWD-VDLPFVGTPVYMSPESVRSGVAEKALDLWSLGCIVLE 216

Query: 222 MMTGRHVWNDCKDNKELISKLIYEREIPTIPKELSELGKDFLEKCFERNYEKRWG----L 281
           M TG   W++ +   E ++  + + + P IPK L    + FLE CF RN ++R      L
Sbjct: 217 MYTGVIPWSEVE--FEDLAPALSKGKAPEIPKSLPCDARKFLETCFSRNPKERGSASDLL 276

Query: 282 SHSSFR--LLSYFSV 284
           SH   R  ++S FS+
Sbjct: 277 SHQFLRGEVVSGFSL 284

BLAST of Moc08g05650 vs. TAIR 10
Match: AT2G41930.1 (Protein kinase superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 4.6e-38
Identity = 106/266 (39.85%), Postives = 150/266 (56.39%), Query Frame = 0

Query: 22  VLGKGSHGKV--FLGELDLGSV---AIKVAPCRAASSLVKEKAILKQFIGCSKIVQYIGD 81
           VLGKG++G V  F  + + GS+   A+K+       S+ +E  IL +  GC  IVQ  G+
Sbjct: 10  VLGKGTYGSVELFSHKQNDGSLLYNAVKIMDSENYGSIDQEFRILSELRGCPCIVQLCGN 69

Query: 82  TVTKKGD---DVVYSLILEYATGGTLGDII-RKQEKISEEDAKKYLRMILQGLSCIHAKG 141
           ++ +  D     VY + +EYA  GTL + I R + K+S+   K + RMILQGL  IH  G
Sbjct: 70  SLVQGIDCNGKKVYMMSMEYAAAGTLTNFIKRNRTKLSDSVIKDFTRMILQGLVSIHNHG 129

Query: 142 FVHADLKSDNILAF-----RSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTA 201
           +VH DLK DNIL F      + +   +LKI+DFG+S       +KS CW   +  +VGT+
Sbjct: 130 YVHCDLKPDNILLFPLYDKDTWNCSYELKISDFGIS---TRAGDKSGCWRVDE-PWVGTS 189

Query: 202 KYMSPESVFD--IVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTIP 261
            YMSPESV D   V+  LD+WSLGCI + M TG+  W   + +   +  L+  ++ P IP
Sbjct: 190 IYMSPESVSDGTTVEKTLDLWSLGCIVLKMYTGKRPWLGFEKD---VKSLLLNQKAPEIP 249

Query: 262 KELSELGKDFLEKCFERNYEKRWGLS 272
           + L    + FLEKCF R  E+R   S
Sbjct: 250 ETLPCDARLFLEKCFSRKPEERGSAS 268

BLAST of Moc08g05650 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 153.7 bits (387), Expect = 2.3e-37
Identity = 87/256 (33.98%), Postives = 144/256 (56.25%), Query Frame = 0

Query: 16  DWDKTPVLGKGSHGKVFLGELDLGSV-AIKVAPCRAASSLVKEKAILKQFIGCSKIVQYI 75
           +W + P++G+GS   V +     G + A+K A   ++S L KE++IL   +    +V+YI
Sbjct: 4   NWTRGPIIGRGSTATVSIAISSSGELFAVKSADLSSSSLLQKEQSILST-LSSPHMVKYI 63

Query: 76  GDTVTKKGDDVVYSLILEYATGGTLGDIIRKQ-EKISEEDAKKYLRMILQGLSCIHAKGF 135
           G  +T++ + +VY++++EY +GG L D+I+    K+ E + + Y R IL GL  +H +G 
Sbjct: 64  GTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHERGI 123

Query: 136 VHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTAKYMSPE 195
           VH DLKS N+L    ++G   LKIAD G ++  D+ E            F GT  +M+PE
Sbjct: 124 VHCDLKSHNVLV--EENG--VLKIADMGCAKSVDKSE------------FSGTPAFMAPE 183

Query: 196 -SVFDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDNKELISKLIYEREIPTIPKELSELG 255
            +  +  +   D+W+LGC  + MMTG   W +  D    + K+ +  E P IP  +S+  
Sbjct: 184 VARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKA 242

Query: 256 KDFLEKCFERNYEKRW 269
           KDFL+ C + + ++RW
Sbjct: 244 KDFLKNCLKEDQKQRW 242

BLAST of Moc08g05650 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 148.3 bits (373), Expect = 9.5e-36
Identity = 93/269 (34.57%), Postives = 145/269 (53.90%), Query Frame = 0

Query: 16  DWDKTPVLGKGSHGKVFLGELDLGS-------VAIKVAPCRAASSLVKEKAILKQFIGCS 75
           +W +   +G GS   V L      S       +A+K +    +++L  E+ +L     CS
Sbjct: 2   EWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCS 61

Query: 76  KIVQYIGDTVTKKGDDVVYSLILEYATGGTLGDIIRKQ-EKISEEDAKKYLRMILQGLSC 135
           +IV+  G+  T +  + +Y+L LEYA+GG+L D I+   E + E + +++ R I++GL  
Sbjct: 62  EIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLCH 121

Query: 136 IHAKGFVHADLKSDNILAFRSKSGRMKLKIADFGLSRMYDEKEEKSLCWNYYKMSFVGTA 195
           IH  GF H D+K +N+L F    G   +KI+DFGL++    +    +C     +   GT 
Sbjct: 122 IHGNGFTHCDIKLENVLVF----GDGDVKISDFGLAK----RRSGEVC-----VEIRGTP 181

Query: 196 KYMSPESV--FDIVKPALDIWSLGCIFVNMMTGRHVWNDCKDN------KELISKLIYER 255
            YM+PESV   +   PA DIW+LGC  V M +G+  W  C ++        L+ ++    
Sbjct: 182 LYMAPESVNHGEFESPA-DIWALGCSVVEMSSGKTAW--CLEDGVMNNVMSLLVRIGSGD 241

Query: 256 EIPTIPKELSELGKDFLEKCFERNYEKRW 269
           E+P IP ELSE GKDF+ KCF +N  +RW
Sbjct: 242 EVPRIPVELSEEGKDFVSKCFVKNAAERW 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131646.11.5e-152100.00mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_022131647.17.4e-9967.39mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_038885921.15.0e-7154.37mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
XP_022150204.12.1e-6954.83mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_022154080.16.3e-6653.31mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SND61.1e-3335.09Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808886.4e-2932.18Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q013899.3e-2833.33Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (str... [more]
Q9CAD54.6e-2733.46Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
Q54R823.0e-2631.56Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1BQ317.4e-153100.00mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A6J1BRL73.6e-9967.39mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A6J1D9A61.0e-6954.83mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A6J1DIL53.1e-6653.31mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A6J1DHA92.6e-6551.15mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
Match NameE-valueIdentityDescription
AT5G55090.19.2e-3936.19mitogen-activated protein kinase kinase kinase 15 [more]
AT2G42550.13.5e-3840.00Protein kinase superfamily protein [more]
AT2G41930.14.6e-3839.85Protein kinase superfamily protein [more]
AT4G26890.12.3e-3733.98mitogen-activated protein kinase kinase kinase 16 [more]
AT4G36950.19.5e-3634.57mitogen-activated protein kinase kinase kinase 21 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 17..281
e-value: 1.5E-53
score: 193.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 20..271
e-value: 2.1E-48
score: 165.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 17..281
score: 36.018391
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 10..279
e-value: 1.1E-55
score: 191.1
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 41..267
e-value: 2.9E-13
score: 47.3
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 16..269
NoneNo IPR availablePANTHERPTHR48011:SF51PROTEIN KINASE SUPERFAMILY PROTEINcoord: 16..269
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 23..44
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 11..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g05650.1Moc08g05650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds
molecular_function GO:0004672 protein kinase activity