Moc08g04550 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g04550
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionL-ascorbate peroxidase
Locationchr8: 3286076 .. 3289651 (-)
RNA-Seq ExpressionMoc08g04550
SyntenyMoc08g04550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCACCTTCCTCAACTCCGCGACGCCGTGCAATGCCGCCGTCGTGTCCTCTTCCTCTCTCAGATTCAAGTTCAAACTTAAATTTCCCGCCAAAGCACCGCTACCGTCTCCTCCGACGGTCCTGTTTCGCGCCAAAACTCCCGTTACTTGTTTTATCCCCGCCCACGGCGATGCTTCTGGTAAACCATTAGTTATCTTCTTTCTTCTTTTTTTTCGTTTGTGCAAAACCTTGGTGGATTGGGAGAGATGCAGCATTGCCCGGTTCGGTTTTCATTACTTGAATTTGACTGTTGTTATCTACCCTGTGTATATTTGCTATAAACGAACAAATTGGTTTGCGGCTGAAACCGAATTCTGTATTTTGCATCATGGAATGGCGAATTGAACCCTACTATTCGCGGAACTAGTAGTTGTTATCTTTATATTGTTTCTTTCGGTCCCTAAAATACTGTTTGAAATTGAATTTACAGTTCAGAATGATTTCTGCTTTCTGAAGAAGAGGGAATTTGTCTTTTCTGCCGCGCTGCCATTTCTTCTTATTTTGCATGGATCCGTGGAAGGTTTTGGAGCTGAGGCGGCAGAGTAAGATGAGGATGGTGCTATTAATTACATGCGATGAAATTTTTAAGTATTTAGCATCTAGCTTTCTGTATTTTTAACTCTGAACATTATCGAGAATTCATGAAGAATGTGGTCCATCTCCATTCATCAGTTAACATTATATATTCCATATATTTTTAGATCCTAGTAGAGGGACCATTAACAACAAATTCTGTCTATTTTCTATTCTGCTCAGAAGATTGGATAAAAGTGAAGATGAACGGATAAGAGAAGAAGTACGAAAGGTGGTAACCAAATACAATGCTGCTGGTTTGCTTCGTCTGGTATTTCACGATGCAGGAACTTTTGAAACGAATGATAATTCAGGTTCATATTCTTATTTTAGGAGAGAGATTTTTAGTTTTGAATGCCGGACAGTGGACTATCAAAACTCGAACAACACTGGATGTGTTGGATTTTAGTTTTTGTTTGCATCATACGCCACTTTTACTGAGATTATGGAGGGCAGTAATGTTCCTTCTTTATAGGCTTTTCGATATGTTTGTCATTTTAGCAAGCTTTGTAATTCTTATGTAGGTGGTATGAATGGGTCTATTATTCACGAACTTGATAGACCTGAAAACACAGGTCTCAAGAAACCAGTTAAGGTGATTTTCTCTTTTATTGGGCCCTCGGTGTTTTATGGATCTCTTCCCAGCAAGCTCATTACTGGTTGCACTGTTTTTAGATTCTTGGGAAAGCAAAGAGTACACTGGATTTAATAAGACCAGGTATATTTCCTCTCTATTTCTCCATTACTGTCAAAGGGAAATAAAGTTTTCATAATAGAAGGTACTAACCAGGCTAACTTCTTGAGAAATTGGCTTGTGAATAGTATCTTGGGCAGACATGATATCAGTGGCTGGCACTGAAGCAGTTTCAATCTGTGGAGGTCCAAGTATTGCTGTTGAGTTGGGTAGATTAGACTCGGGGTAAGGTGAAGGTGGCTGTTTCTATTGTTATATGATGAATTAACATTTTACTTGAGATGCAATCTTTTAACTTGTTTCTCTTTGCTGTTGTTTTTCCATGAGCTTTATGCACGAGAATGGTTCATTTGGTTTTCTGGACAATAATGACTAATGAGTATATTCTCCGCATTCTAACCTTTCCCATGAGCTTTATGAACAAGGGAGAGTTCCGATTTCTGAGAATCTCCCTCTCTAATATTCAACTAGTTGATTTCCTGTTCCACATTATAGTTTGATCCGTCCTTACTATATGCACACTCTCTCTTCTTCATCAATTATACCTTATGCTAACTAGCTTCTAATTGAGTTAGTCCTCATGTGCAGTAGACCAGTCTAGCTAACTAACTTCCTGTGTTCCCTTTACTGAAATTTTTGTCTCCCATTATTGCATTCTTTTTGTCATATAAGTAAAAATTGGCTATGAGGTTGGTCGTATCATATTAATGTTAGATATGTATGGGTGTGTGTTTTTTTTTCCTTTCATAATTTTTTCCCTCCTTTTCCAAATAGGAAGCCAGATCCTGAAGGAAAACTTCCAGAGGAATCTTTGGATGCTTTTGGATTGAAGCAAAGCTTCAGCAGAAAAGGATTTGCGTGAGTGCATCTGCTATCTCATTTTGACCCTGCCGTTTGAAGGAATTGTTTATTTCTTTTAAATGATCTTCTGCAAAGTCACTAAACTGTTTTATATTTGGCTTTTATTTTCATTTGCATCTTTTTTTCTTTGCCAATATATTCTATGTTACTGCATCTCTCTCATTCATATATTCACTTCCTAATGTGGCTGAGTTGTGATTATGGTTTCTAAATTATTATTTGGATTCCTTTTCTGCTACATCTGTTTTTGAGAATACAGAAAGTGACTCAAAGTCAATTATCAAAATAAACATTTAGTAATGGGAAATACTAGTTCTACATTTGATTGCACGACACAATCCTTTTAAATATGTGTTGAAAAATCTTGTTAATTATGAAAACCAACTACCTCCTAGTACCAACAAGTATAACATGGACAGTAGAAGAGAAGTTAACAATGATGCTGTCACTCTTGAAATCTGCCCATTATCTGTCAAATCCTTCACCCAGACACAAAAGTGCAAATACCAAACAATTCATAATGGATGCTGTTTTTCTTTGCAGAACACAGGAACTAGTCGCTCTATCTGGAGCCCATACCATTGGCAGTAAAGGTTTTGGAAGCCCTGTTGTTTTTGATAATTCGTATTTCAAAATCCTTTTGGAGAAGCCATGGTCGGATACTGGTAATCAATCTCATGACAAAATTTCCATTCTTCTCATGTTGTTCGAATCAATCCGTCCTTGGCTACATGAGGCAATGCCTCAACTCATGCTCTAATCATATTTAATTCTGAACTGCTTAGATAGGTTTTGGTACTATCTTAGCGAGTAAATGAAATATTATCAATTTATCATTATGCTATTCTGGCTGAACTGAGCTAAGAAATTCTTGTGATTTCTGGGAACTGAGTTGTTAGTTTTGCTATTGATTAATAATACAAGGAACTGCTCTGAATTAGTTAATACATATGTCCACTCAGATCTTATTTTACTGTTTATTTTTAGGTGGTATGTCCAGTATGATTGGGCTGCCTTCAGATCGTGCTCTCACTGACGACGATGAATGCTTGAGGTTCTTCTCTTTCATTCAAACCTATTCTACTTTCACCTAAATTTTCTACACCACTGTAAAATGATGGCATTTCTGGTCTCTTAAGTAGAATTTTAAGAATGAAATCAATTGGTTTGGCTAGAGTAGAAAACTTCCTTAAAAGTTAAACTTTCCAACCTGGCAACCACCGAGATCCAACCAATAGAGAGAAAATTAGCTGATTGTTACTCTTTATCTGCTCAACTGAAGTGGATTACTTTGAACTCGTAGGTGGATCAAAGAGTACGCCAACAACCAGAATGTGTTCTTTGAAGATTTCAAGAAGGCTTATATCAAGCTAGTAAATTCTGGTGCAAAGTGGAAAAGCTAG

mRNA sequence

ATGGCTTCCACCTTCCTCAACTCCGCGACGCCGTGCAATGCCGCCGTCGTGTCCTCTTCCTCTCTCAGATTCAAGTTCAAACTTAAATTTCCCGCCAAAGCACCGCTACCGTCTCCTCCGACGGTCCTGTTTCGCGCCAAAACTCCCGTTACTTGTTTTATCCCCGCCCACGGCGATGCTTCTGTTCAGAATGATTTCTGCTTTCTGAAGAAGAGGGAATTTGTCTTTTCTGCCGCGCTGCCATTTCTTCTTATTTTGCATGGATCCGTGGAAGGTTTTGGAGCTGAGGCGGCAGAAAGATTGGATAAAAGTGAAGATGAACGGATAAGAGAAGAAGTACGAAAGGTGGTAACCAAATACAATGCTGCTGGTTTGCTTCGTCTGGTATTTCACGATGCAGGAACTTTTGAAACGAATGATAATTCAGGTGGTATGAATGGGTCTATTATTCACGAACTTGATAGACCTGAAAACACAGGTCTCAAGAAACCAGTTAAGATTCTTGGGAAAGCAAAGAGTACACTGGATTTAATAAGACCAGTATCTTGGGCAGACATGATATCAGTGGCTGGCACTGAAGCAGTTTCAATCTGTGGAGGTCCAAGTATTGCTGTTGAGTTGGGTAGATTAGACTCGGGGAAGCCAGATCCTGAAGGAAAACTTCCAGAGGAATCTTTGGATGCTTTTGGATTGAAGCAAAGCTTCAGCAGAAAAGGATTTGCAACACAGGAACTAGTCGCTCTATCTGGAGCCCATACCATTGGCAGTAAAGGTTTTGGAAGCCCTGTTGTTTTTGATAATTCGTATTTCAAAATCCTTTTGGAGAAGCCATGGTCGGATACTGGTGGTATGTCCAGTATGATTGGGCTGCCTTCAGATCGTGCTCTCACTGACGACGATGAATGCTTGAGGTGGATCAAAGAGTACGCCAACAACCAGAATGTGTTCTTTGAAGATTTCAAGAAGGCTTATATCAAGCTAGTAAATTCTGGTGCAAAGTGGAAAAGCTAG

Coding sequence (CDS)

ATGGCTTCCACCTTCCTCAACTCCGCGACGCCGTGCAATGCCGCCGTCGTGTCCTCTTCCTCTCTCAGATTCAAGTTCAAACTTAAATTTCCCGCCAAAGCACCGCTACCGTCTCCTCCGACGGTCCTGTTTCGCGCCAAAACTCCCGTTACTTGTTTTATCCCCGCCCACGGCGATGCTTCTGTTCAGAATGATTTCTGCTTTCTGAAGAAGAGGGAATTTGTCTTTTCTGCCGCGCTGCCATTTCTTCTTATTTTGCATGGATCCGTGGAAGGTTTTGGAGCTGAGGCGGCAGAAAGATTGGATAAAAGTGAAGATGAACGGATAAGAGAAGAAGTACGAAAGGTGGTAACCAAATACAATGCTGCTGGTTTGCTTCGTCTGGTATTTCACGATGCAGGAACTTTTGAAACGAATGATAATTCAGGTGGTATGAATGGGTCTATTATTCACGAACTTGATAGACCTGAAAACACAGGTCTCAAGAAACCAGTTAAGATTCTTGGGAAAGCAAAGAGTACACTGGATTTAATAAGACCAGTATCTTGGGCAGACATGATATCAGTGGCTGGCACTGAAGCAGTTTCAATCTGTGGAGGTCCAAGTATTGCTGTTGAGTTGGGTAGATTAGACTCGGGGAAGCCAGATCCTGAAGGAAAACTTCCAGAGGAATCTTTGGATGCTTTTGGATTGAAGCAAAGCTTCAGCAGAAAAGGATTTGCAACACAGGAACTAGTCGCTCTATCTGGAGCCCATACCATTGGCAGTAAAGGTTTTGGAAGCCCTGTTGTTTTTGATAATTCGTATTTCAAAATCCTTTTGGAGAAGCCATGGTCGGATACTGGTGGTATGTCCAGTATGATTGGGCTGCCTTCAGATCGTGCTCTCACTGACGACGATGAATGCTTGAGGTGGATCAAAGAGTACGCCAACAACCAGAATGTGTTCTTTGAAGATTTCAAGAAGGCTTATATCAAGCTAGTAAATTCTGGTGCAAAGTGGAAAAGCTAG

Protein sequence

MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDASVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKYNAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGFATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS
Homology
BLAST of Moc08g04550 vs. NCBI nr
Match: XP_022131497.1 (putative L-ascorbate peroxidase 6 isoform X1 [Momordica charantia] >XP_022131499.1 putative L-ascorbate peroxidase 6 isoform X1 [Momordica charantia])

HSP 1 Score: 669.8 bits (1727), Expect = 1.2e-188
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA
Sbjct: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY
Sbjct: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
           NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP
Sbjct: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF
Sbjct: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD
Sbjct: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300

Query: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS
Sbjct: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 336

BLAST of Moc08g04550 vs. NCBI nr
Match: XP_022131500.1 (putative L-ascorbate peroxidase 6 isoform X2 [Momordica charantia])

HSP 1 Score: 663.7 bits (1711), Expect = 8.5e-187
Identity = 335/336 (99.70%), Postives = 335/336 (99.70%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA
Sbjct: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAE LDKSEDERIREEVRKVVTKY
Sbjct: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAE-LDKSEDERIREEVRKVVTKY 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
           NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP
Sbjct: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF
Sbjct: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD
Sbjct: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300

Query: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS
Sbjct: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 335

BLAST of Moc08g04550 vs. NCBI nr
Match: XP_022131501.1 (putative L-ascorbate peroxidase 6 isoform X3 [Momordica charantia])

HSP 1 Score: 602.8 bits (1553), Expect = 1.8e-168
Identity = 304/305 (99.67%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA
Sbjct: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY
Sbjct: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
           NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP
Sbjct: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF
Sbjct: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD
Sbjct: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300

Query: 301 ECLRW 306
           ECLR+
Sbjct: 301 ECLRF 305

BLAST of Moc08g04550 vs. NCBI nr
Match: XP_038885450.1 (putative L-ascorbate peroxidase 6 isoform X1 [Benincasa hispida] >XP_038885451.1 putative L-ascorbate peroxidase 6 isoform X1 [Benincasa hispida])

HSP 1 Score: 552.7 bits (1423), Expect = 2.1e-153
Identity = 285/336 (84.82%), Postives = 295/336 (87.80%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           M STF N A PCNAAVVSSS LRF F  KFPA  PL S  TV FRAKT V C +PAHGDA
Sbjct: 1   MTSTFFNPAPPCNAAVVSSSFLRFGFIPKFPAIIPLSSLSTVPFRAKTTVNCSLPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQ DFCFLKKREFVFSAALPFLLIL  SVEGF AEAAERLDK E ERIREEVRKVVTK 
Sbjct: 61  SVQTDFCFLKKREFVFSAALPFLLILAESVEGFKAEAAERLDKREVERIREEVRKVVTKG 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
            A GLLRLVFHDAGTFE ND SGGMNGSIIHELDRPEN GLKK VKIL +AKSTLDLIRP
Sbjct: 121 RAPGLLRLVFHDAGTFEMNDTSGGMNGSIIHELDRPENRGLKKSVKILEEAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWAD+I+VAG EAVS+CGGPSI+VELGRLDS KPDPEGKLPEESLDA GLKQ FSRKGF
Sbjct: 181 VSWADVIAVAGAEAVSVCGGPSISVELGRLDSEKPDPEGKLPEESLDAVGLKQIFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           AT+ELVALSGAHTIG KGFGSPVVFDN+YFKILLEKPWS  GGMSSMIGLPSD AL DDD
Sbjct: 241 ATRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDHALADDD 300

Query: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           ECLRWIKEYAN+QNVFFEDF+ AYIKLVNSGAKWKS
Sbjct: 301 ECLRWIKEYANDQNVFFEDFQNAYIKLVNSGAKWKS 336

BLAST of Moc08g04550 vs. NCBI nr
Match: XP_038885452.1 (putative L-ascorbate peroxidase 6 isoform X2 [Benincasa hispida])

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-151
Identity = 284/336 (84.52%), Postives = 294/336 (87.50%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           M STF N A PCNAAVVSSS LRF F  KFPA  PL S  TV FRAKT V C +PAHGDA
Sbjct: 1   MTSTFFNPAPPCNAAVVSSSFLRFGFIPKFPAIIPLSSLSTVPFRAKTTVNCSLPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQ DFCFLKKREFVFSAALPFLLIL  SVEGF AEAAE LDK E ERIREEVRKVVTK 
Sbjct: 61  SVQTDFCFLKKREFVFSAALPFLLILAESVEGFKAEAAE-LDKREVERIREEVRKVVTKG 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
            A GLLRLVFHDAGTFE ND SGGMNGSIIHELDRPEN GLKK VKIL +AKSTLDLIRP
Sbjct: 121 RAPGLLRLVFHDAGTFEMNDTSGGMNGSIIHELDRPENRGLKKSVKILEEAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWAD+I+VAG EAVS+CGGPSI+VELGRLDS KPDPEGKLPEESLDA GLKQ FSRKGF
Sbjct: 181 VSWADVIAVAGAEAVSVCGGPSISVELGRLDSEKPDPEGKLPEESLDAVGLKQIFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           AT+ELVALSGAHTIG KGFGSPVVFDN+YFKILLEKPWS  GGMSSMIGLPSD AL DDD
Sbjct: 241 ATRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDHALADDD 300

Query: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           ECLRWIKEYAN+QNVFFEDF+ AYIKLVNSGAKWKS
Sbjct: 301 ECLRWIKEYANDQNVFFEDFQNAYIKLVNSGAKWKS 335

BLAST of Moc08g04550 vs. ExPASy Swiss-Prot
Match: Q8GY91 (Putative L-ascorbate peroxidase 6 OS=Arabidopsis thaliana OX=3702 GN=APX6 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-96
Identity = 189/317 (59.62%), Postives = 224/317 (70.66%), Query Frame = 0

Query: 18  SSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDASVQNDFCFLKKREFVFS 77
           S SS +FK K + PAK  L SP T     ++     I    D    +      +R+ V  
Sbjct: 18  SFSSFKFKCKFESPAKTRLLSPATEKHVVRSSRAWRIRCLSDDPGSSHVFVASRRKMVVL 77

Query: 78  AALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKYNAAGLLRLVFHDAGTFE 137
            +   LL       G  AE    +        + E+RKVVTK  AAG+LRLVFHDAGTFE
Sbjct: 78  LSTVQLLSHMLPQNGNAAEIYPVM--------QNEIRKVVTKGKAAGVLRLVFHDAGTFE 137

Query: 138 TNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSI 197
            +D+SGG+NGSI +EL+RPEN GLKK +K+L KAK  +D I+PVSWADMISVAG+EAVSI
Sbjct: 138 LDDHSGGINGSIAYELERPENIGLKKSLKVLAKAKVKVDEIQPVSWADMISVAGSEAVSI 197

Query: 198 CGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGFATQELVALSGAHTIGSK 257
           CGGP+I V LGRLDS +PDPEGKLP E+L A GLK+ F RKGF+TQELVALSGAHTIGSK
Sbjct: 198 CGGPTIPVVLGRLDSAQPDPEGKLPPETLSASGLKECFKRKGFSTQELVALSGAHTIGSK 257

Query: 258 GFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFF 317
           GFG P VFDN+Y+KILLEKPW+ T  M+SM+GLPSD AL  DDECLRW+K YA +Q+ FF
Sbjct: 258 GFGDPTVFDNAYYKILLEKPWTSTSKMTSMVGLPSDHALVQDDECLRWVKRYAEDQDKFF 317

Query: 318 EDFKKAYIKLVNSGAKW 335
           EDF  AYIKLVNSGAKW
Sbjct: 318 EDFTNAYIKLVNSGAKW 326

BLAST of Moc08g04550 vs. ExPASy Swiss-Prot
Match: Q1PER6 (L-ascorbate peroxidase 2, cytosolic OS=Arabidopsis thaliana OX=3702 GN=APX2 PE=2 SV=3)

HSP 1 Score: 146.7 bits (369), Expect = 4.6e-34
Identity = 91/242 (37.60%), Postives = 138/242 (57.02%), Query Frame = 0

Query: 103 KSEDERIREEVRKVVT-KYNAAGLLRLVFHDAGTFETNDNSGGMNGSIIH--ELDRPENT 162
           K   +R + ++R ++  K+ A  +LRL +H AGTF+    +GG  G+I H  EL    N 
Sbjct: 14  KKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANN 73

Query: 163 GLKKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEG 222
           GL   V++L   K   +L   +S+AD   +AG  AV I GGP I    GRLD  +P PEG
Sbjct: 74  GLDIAVRLLDPIK---ELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEG 133

Query: 223 KLPEESLDAFGLKQSFSRKGFATQELVALSGAHTIG-----SKGF-----GSPVVFDNSY 282
           +LP+ +     L+  F R G   +++VALSG HT+G       GF      +P++FDNSY
Sbjct: 134 RLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSY 193

Query: 283 FKILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVN 332
           FK +L      +G    ++ LP+D+AL DD   L ++++YA +++ FFED+ +A++KL  
Sbjct: 194 FKEIL------SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSE 246

BLAST of Moc08g04550 vs. ExPASy Swiss-Prot
Match: P48534 (L-ascorbate peroxidase, cytosolic OS=Pisum sativum OX=3888 GN=APX1 PE=1 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-31
Identity = 89/239 (37.24%), Postives = 133/239 (55.65%), Query Frame = 0

Query: 107 ERIREEVRKVVTKYNAAGL-LRLVFHDAGTFETNDNSGGMNGSIIH--ELDRPENTGLKK 166
           E+ + ++R  + +   A L LRL +H AGTF++   +GG  G+I H  EL    N GL  
Sbjct: 17  EKAKRKLRGFIAEKKCAPLILRLAWHSAGTFDSKTKTGGPFGTIKHQAELAHGANNGLDI 76

Query: 167 PVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPE 226
            V++L   K    +   VS+AD   +AG  AV I GGP +    GR D  +P PEG+LP+
Sbjct: 77  AVRLLEPIKEQFPI---VSYADFYQLAGVVAVEITGGPEVPFHPGREDKPEPPPEGRLPD 136

Query: 227 ESLDAFGLKQSFSR-KGFATQELVALSGAHTIGS-----KGF-----GSPVVFDNSYFKI 286
            +  +  L+  F +  G + Q++VALSG HTIG+      GF      +P++FDNSYF  
Sbjct: 137 ATKGSDHLRDVFGKAMGLSDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTE 196

Query: 287 LLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVNSG 332
           LL      TG    ++ LPSD+AL  D      +++YA +++VFF D+ +A++KL   G
Sbjct: 197 LL------TGEKDGLLQLPSDKALLTDSVFRPLVEKYAADEDVFFADYAEAHLKLSELG 246

BLAST of Moc08g04550 vs. ExPASy Swiss-Prot
Match: A2XFC7 (L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=APX1 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.6e-31
Identity = 87/239 (36.40%), Postives = 136/239 (56.90%), Query Frame = 0

Query: 107 ERIREEVRKVVTKYNAAGL-LRLVFHDAGTFETNDNSGGMNGSI--IHELDRPENTGLKK 166
           E+ R+++R ++ + + A L LRL +H AGTF+ +  +GG  G++    EL    N GL  
Sbjct: 17  EKARQKLRALIAEKSCAPLMLRLAWHSAGTFDVSSKTGGPFGTMKTPAELSHAANAGLDI 76

Query: 167 PVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPE 226
            V++L   K   + I  +S+AD   +AG  AV + GGP++    GR D   P PEG+LP+
Sbjct: 77  AVRMLEPIK---EEIPTISYADFYQLAGVVAVEVSGGPAVPFHPGREDKPAPPPEGRLPD 136

Query: 227 ESLDAFGLKQSF-SRKGFATQELVALSGAHTIG-----SKGF-----GSPVVFDNSYFKI 286
            +  +  L+Q F ++ G + Q++VALSG HT+G       GF      +P+ FDNSYF  
Sbjct: 137 ATKGSDHLRQVFGAQMGLSDQDIVALSGGHTLGRCHKERSGFEGPWTRNPLQFDNSYFTE 196

Query: 287 LLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVNSG 332
           LL      +G    ++ LPSD+AL  D      +++YA ++  FFED+K+A++KL   G
Sbjct: 197 LL------SGDKEGLLQLPSDKALLSDPAFCPLVEKYAADEKAFFEDYKEAHLKLSELG 246

BLAST of Moc08g04550 vs. ExPASy Swiss-Prot
Match: Q10N21 (L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=APX1 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.7e-31
Identity = 87/239 (36.40%), Postives = 136/239 (56.90%), Query Frame = 0

Query: 107 ERIREEVRKVVTKYNAAGL-LRLVFHDAGTFETNDNSGGMNGSI--IHELDRPENTGLKK 166
           E+ R+++R ++ + + A L LRL +H AGTF+ +  +GG  G++    EL    N GL  
Sbjct: 17  EKARQKLRALIAEKSCAPLMLRLAWHSAGTFDVSSKTGGPFGTMKTPAELSHAANAGLDI 76

Query: 167 PVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPE 226
            V++L   K   + I  +S+AD   +AG  AV + GGP++    GR D   P PEG+LP+
Sbjct: 77  AVRMLEPIK---EEIPTISYADFYQLAGVVAVEVSGGPAVPFHPGREDKPAPPPEGRLPD 136

Query: 227 ESLDAFGLKQSF-SRKGFATQELVALSGAHTIG-----SKGF-----GSPVVFDNSYFKI 286
            +  +  L+Q F ++ G + Q++VALSG HT+G       GF      +P+ FDNSYF  
Sbjct: 137 ATKGSDHLRQVFGAQMGLSDQDIVALSGGHTLGRCHKERSGFEGPWTRNPLQFDNSYFTE 196

Query: 287 LLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVNSG 332
           LL      +G    ++ LPSD+AL  D      +++YA ++  FFED+K+A++KL   G
Sbjct: 197 LL------SGDKEGLLQLPSDKALLSDPAFRPLVEKYAADEKAFFEDYKEAHLKLSELG 246

BLAST of Moc08g04550 vs. ExPASy TrEMBL
Match: A0A6J1BR64 (L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111004683 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 5.7e-189
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA
Sbjct: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY
Sbjct: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
           NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP
Sbjct: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF
Sbjct: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD
Sbjct: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300

Query: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS
Sbjct: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 336

BLAST of Moc08g04550 vs. ExPASy TrEMBL
Match: A0A6J1BPV9 (L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111004683 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.1e-187
Identity = 335/336 (99.70%), Postives = 335/336 (99.70%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA
Sbjct: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAE LDKSEDERIREEVRKVVTKY
Sbjct: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAE-LDKSEDERIREEVRKVVTKY 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
           NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP
Sbjct: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF
Sbjct: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD
Sbjct: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300

Query: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS
Sbjct: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 335

BLAST of Moc08g04550 vs. ExPASy TrEMBL
Match: A0A6J1BPN9 (L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111004683 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 8.6e-169
Identity = 304/305 (99.67%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA
Sbjct: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY
Sbjct: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
           NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP
Sbjct: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF
Sbjct: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD
Sbjct: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300

Query: 301 ECLRW 306
           ECLR+
Sbjct: 301 ECLRF 305

BLAST of Moc08g04550 vs. ExPASy TrEMBL
Match: A0A0A0LQ87 (L-ascorbate peroxidase OS=Cucumis sativus OX=3659 GN=Csa_2G381780 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.1e-150
Identity = 280/338 (82.84%), Postives = 292/338 (86.39%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           M STF N A  CN AV+SSS LRFKF  KFP   P  S  TV FRAKT V C +PA G A
Sbjct: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAE--RLDKSEDERIREEVRKVVT 120
           SVQ DFCFLKKREFVFSAALPFLLILH SVEGF AEAAE  RLDK+E ERIREEVRKVVT
Sbjct: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120

Query: 121 KYNAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLI 180
           K  A GLLRLVFHDAGTFETND SGGMNGSI+HELDRPEN GLKK VKIL +AKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRK 240
           RPVSWAD+I VAG EAVSICGGPSIAV+LGRLDS KPDPEGKLPEESLDA GLKQSFSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240

Query: 241 GFATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTD 300
           GF+T+ELVALSGAHTIG KGFGSPVVFDN+YFKILLEKPWS  GGMSSMIGLPSDRAL D
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           DDECLRWIKEYA +QNVFFEDF  AYIKLVNSGAKW+S
Sbjct: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338

BLAST of Moc08g04550 vs. ExPASy TrEMBL
Match: A0A5A7V9T2 (L-ascorbate peroxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002410 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 281/336 (83.63%), Postives = 291/336 (86.61%), Query Frame = 0

Query: 1   MASTFLNSATPCNAAVVSSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDA 60
           M STF N A PCN AVVSSS LRFKF  KFPA  PL S  TV FRAKT V   + A GDA
Sbjct: 1   MISTFFNPAPPCNVAVVSSSFLRFKFIPKFPATVPLSSLSTVSFRAKTTVNRSLCAPGDA 60

Query: 61  SVQNDFCFLKKREFVFSAALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKY 120
           SVQ DFC LKKREFVFSAALPFLLILH SVEGF AEAAERLDK E ERIREEVRKVVTK 
Sbjct: 61  SVQTDFCLLKKREFVFSAALPFLLILHESVEGFKAEAAERLDKREVERIREEVRKVVTKG 120

Query: 121 NAAGLLRLVFHDAGTFETNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRP 180
            A GLLRLVFHDAGTFETND SGGMNGSIIHELDRPEN GLKK VKIL +AKSTLDLIRP
Sbjct: 121 RAPGLLRLVFHDAGTFETNDTSGGMNGSIIHELDRPENKGLKKSVKILQEAKSTLDLIRP 180

Query: 181 VSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGF 240
           VSWAD+I VAG EAVSICGGPSIAV+LGRLDS KPD EGKLPEESLDA GLKQ FSRKGF
Sbjct: 181 VSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDLEGKLPEESLDAVGLKQIFSRKGF 240

Query: 241 ATQELVALSGAHTIGSKGFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDD 300
           +T+ELVALSGAHTIG KGFGSPVVFDN+YFKILLEKPWS  GGMSSMIGLPSDRAL DDD
Sbjct: 241 STRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALADDD 300

Query: 301 ECLRWIKEYANNQNVFFEDFKKAYIKLVNSGAKWKS 337
           ECLRWIKEYA +QNVFFEDF+ AYIKLVNSGAKW+S
Sbjct: 301 ECLRWIKEYAKDQNVFFEDFQNAYIKLVNSGAKWRS 336

BLAST of Moc08g04550 vs. TAIR 10
Match: AT4G32320.1 (ascorbate peroxidase 6 )

HSP 1 Score: 354.8 bits (909), Expect = 7.9e-98
Identity = 189/317 (59.62%), Postives = 224/317 (70.66%), Query Frame = 0

Query: 18  SSSSLRFKFKLKFPAKAPLPSPPTVLFRAKTPVTCFIPAHGDASVQNDFCFLKKREFVFS 77
           S SS +FK K + PAK  L SP T     ++     I    D    +      +R+ V  
Sbjct: 18  SFSSFKFKCKFESPAKTRLLSPATEKHVVRSSRAWRIRCLSDDPGSSHVFVASRRKMVVL 77

Query: 78  AALPFLLILHGSVEGFGAEAAERLDKSEDERIREEVRKVVTKYNAAGLLRLVFHDAGTFE 137
            +   LL       G  AE    +        + E+RKVVTK  AAG+LRLVFHDAGTFE
Sbjct: 78  LSTVQLLSHMLPQNGNAAEIYPVM--------QNEIRKVVTKGKAAGVLRLVFHDAGTFE 137

Query: 138 TNDNSGGMNGSIIHELDRPENTGLKKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSI 197
            +D+SGG+NGSI +EL+RPEN GLKK +K+L KAK  +D I+PVSWADMISVAG+EAVSI
Sbjct: 138 LDDHSGGINGSIAYELERPENIGLKKSLKVLAKAKVKVDEIQPVSWADMISVAGSEAVSI 197

Query: 198 CGGPSIAVELGRLDSGKPDPEGKLPEESLDAFGLKQSFSRKGFATQELVALSGAHTIGSK 257
           CGGP+I V LGRLDS +PDPEGKLP E+L A GLK+ F RKGF+TQELVALSGAHTIGSK
Sbjct: 198 CGGPTIPVVLGRLDSAQPDPEGKLPPETLSASGLKECFKRKGFSTQELVALSGAHTIGSK 257

Query: 258 GFGSPVVFDNSYFKILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFF 317
           GFG P VFDN+Y+KILLEKPW+ T  M+SM+GLPSD AL  DDECLRW+K YA +Q+ FF
Sbjct: 258 GFGDPTVFDNAYYKILLEKPWTSTSKMTSMVGLPSDHALVQDDECLRWVKRYAEDQDKFF 317

Query: 318 EDFKKAYIKLVNSGAKW 335
           EDF  AYIKLVNSGAKW
Sbjct: 318 EDFTNAYIKLVNSGAKW 326

BLAST of Moc08g04550 vs. TAIR 10
Match: AT3G09640.1 (ascorbate peroxidase 2 )

HSP 1 Score: 146.7 bits (369), Expect = 3.3e-35
Identity = 91/242 (37.60%), Postives = 138/242 (57.02%), Query Frame = 0

Query: 103 KSEDERIREEVRKVVT-KYNAAGLLRLVFHDAGTFETNDNSGGMNGSIIH--ELDRPENT 162
           K   +R + ++R ++  K+ A  +LRL +H AGTF+    +GG  G+I H  EL    N 
Sbjct: 14  KKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANN 73

Query: 163 GLKKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEG 222
           GL   V++L   K   +L   +S+AD   +AG  AV I GGP I    GRLD  +P PEG
Sbjct: 74  GLDIAVRLLDPIK---ELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEG 133

Query: 223 KLPEESLDAFGLKQSFSRKGFATQELVALSGAHTIG-----SKGF-----GSPVVFDNSY 282
           +LP+ +     L+  F R G   +++VALSG HT+G       GF      +P++FDNSY
Sbjct: 134 RLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSY 193

Query: 283 FKILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVN 332
           FK +L      +G    ++ LP+D+AL DD   L ++++YA +++ FFED+ +A++KL  
Sbjct: 194 FKEIL------SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSE 246

BLAST of Moc08g04550 vs. TAIR 10
Match: AT3G09640.2 (ascorbate peroxidase 2 )

HSP 1 Score: 146.7 bits (369), Expect = 3.3e-35
Identity = 91/242 (37.60%), Postives = 138/242 (57.02%), Query Frame = 0

Query: 103 KSEDERIREEVRKVVT-KYNAAGLLRLVFHDAGTFETNDNSGGMNGSIIH--ELDRPENT 162
           K   +R + ++R ++  K+ A  +LRL +H AGTF+    +GG  G+I H  EL    N 
Sbjct: 14  KKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANN 73

Query: 163 GLKKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEG 222
           GL   V++L   K   +L   +S+AD   +AG  AV I GGP I    GRLD  +P PEG
Sbjct: 74  GLDIAVRLLDPIK---ELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEG 133

Query: 223 KLPEESLDAFGLKQSFSRKGFATQELVALSGAHTIG-----SKGF-----GSPVVFDNSY 282
           +LP+ +     L+  F R G   +++VALSG HT+G       GF      +P++FDNSY
Sbjct: 134 RLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSY 193

Query: 283 FKILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVN 332
           FK +L      +G    ++ LP+D+AL DD   L ++++YA +++ FFED+ +A++KL  
Sbjct: 194 FKEIL------SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSE 246

BLAST of Moc08g04550 vs. TAIR 10
Match: AT4G35970.1 (ascorbate peroxidase 5 )

HSP 1 Score: 132.9 bits (333), Expect = 4.9e-31
Identity = 87/240 (36.25%), Postives = 133/240 (55.42%), Query Frame = 0

Query: 105 EDERIREEVRKVVTKYNAAG-LLRLVFHDAGTFETNDNSGGMNGSI--IHELDRPENTGL 164
           E E+ R ++R +++  N A  +LRL +HDAGT++    +GG NGSI    EL+RP N GL
Sbjct: 12  EIEKTRRDLRALISSRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEELNRPHNKGL 71

Query: 165 KKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKL 224
           +K V    + K+       VS+AD+  +AG  AV + GGP+I    GR D+   D +G+L
Sbjct: 72  EKAVAFCEEVKAKHP---RVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSAD-DGEL 131

Query: 225 PEESLDAFGLKQSFSRKGFATQELVALSGAHTIG----------SKGFGSPVVFDNSYFK 284
           P  +  A  L+  FSR G   +++VALSG HT+G                P+ FDNSYF 
Sbjct: 132 PNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFDNSYFV 191

Query: 285 ILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVNSG 332
            LL+      G    ++ L +D+AL DD +   ++K YA ++++FF+ +  ++ KL   G
Sbjct: 192 ELLK------GETPGLLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSELG 241

BLAST of Moc08g04550 vs. TAIR 10
Match: AT4G35000.1 (ascorbate peroxidase 3 )

HSP 1 Score: 129.4 bits (324), Expect = 5.4e-30
Identity = 83/240 (34.58%), Postives = 128/240 (53.33%), Query Frame = 0

Query: 105 EDERIREEVRKVVTKYNAAG-LLRLVFHDAGTFETNDNSGGMNGSIIHELDRPE--NTGL 164
           E  + R E+R ++   N A  +LRL +HDAGT++    +GG NGSI +E +     N+GL
Sbjct: 13  EITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRNEEEHTHGANSGL 72

Query: 165 KKPVKILGKAKSTLDLIRPVSWADMISVAGTEAVSICGGPSIAVELGRLDSGKPDPEGKL 224
           K  + +    K+       +++AD+  +AG  AV + GGP I    GR DS     EG+L
Sbjct: 73  KIALDLCEGVKAKHP---KITYADLYQLAGVVAVEVTGGPDIVFVPGRKDSNVCPKEGRL 132

Query: 225 PEESLDAFGLKQSFSRKGFATQELVALSGAHTIG-----SKGFG-----SPVVFDNSYFK 284
           P+       L+  F R G + +++VALSG HT+G       GF       P+ FDNSYF 
Sbjct: 133 PDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEPLKFDNSYFV 192

Query: 285 ILLEKPWSDTGGMSSMIGLPSDRALTDDDECLRWIKEYANNQNVFFEDFKKAYIKLVNSG 332
            LL+      G    ++ LP+D+ L +D E  R ++ YA +++ FF D+ +++ KL   G
Sbjct: 193 ELLK------GESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSELG 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131497.11.2e-188100.00putative L-ascorbate peroxidase 6 isoform X1 [Momordica charantia] >XP_022131499... [more]
XP_022131500.18.5e-18799.70putative L-ascorbate peroxidase 6 isoform X2 [Momordica charantia][more]
XP_022131501.11.8e-16899.67putative L-ascorbate peroxidase 6 isoform X3 [Momordica charantia][more]
XP_038885450.12.1e-15384.82putative L-ascorbate peroxidase 6 isoform X1 [Benincasa hispida] >XP_038885451.1... [more]
XP_038885452.11.5e-15184.52putative L-ascorbate peroxidase 6 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8GY911.1e-9659.62Putative L-ascorbate peroxidase 6 OS=Arabidopsis thaliana OX=3702 GN=APX6 PE=2 S... [more]
Q1PER64.6e-3437.60L-ascorbate peroxidase 2, cytosolic OS=Arabidopsis thaliana OX=3702 GN=APX2 PE=2... [more]
P485341.2e-3137.24L-ascorbate peroxidase, cytosolic OS=Pisum sativum OX=3888 GN=APX1 PE=1 SV=2[more]
A2XFC73.6e-3136.40L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=AP... [more]
Q10N214.7e-3136.40L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1BR645.7e-189100.00L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111004683 PE=3 SV=1[more]
A0A6J1BPV94.1e-18799.70L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111004683 PE=3 SV=1[more]
A0A6J1BPN98.6e-16999.67L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111004683 PE=3 SV=1[more]
A0A0A0LQ873.1e-15082.84L-ascorbate peroxidase OS=Cucumis sativus OX=3659 GN=Csa_2G381780 PE=3 SV=1[more]
A0A5A7V9T21.2e-14983.63L-ascorbate peroxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
Match NameE-valueIdentityDescription
AT4G32320.17.9e-9859.62ascorbate peroxidase 6 [more]
AT3G09640.13.3e-3537.60ascorbate peroxidase 2 [more]
AT3G09640.23.3e-3537.60ascorbate peroxidase 2 [more]
AT4G35970.14.9e-3136.25ascorbate peroxidase 5 [more]
AT4G35000.15.4e-3034.58ascorbate peroxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002207Class I peroxidasePRINTSPR00459ASPEROXIDASEcoord: 180..198
score: 36.44
coord: 220..237
score: 26.28
coord: 238..259
score: 44.58
coord: 140..150
score: 34.97
coord: 199..211
score: 50.3
coord: 122..137
score: 53.61
coord: 308..332
score: 34.31
IPR002016Haem peroxidasePRINTSPR00458PEROXIDASEcoord: 199..211
score: 50.78
coord: 244..259
score: 48.9
coord: 122..136
score: 47.95
coord: 181..198
score: 36.99
IPR002016Haem peroxidasePFAMPF00141peroxidasecoord: 110..312
e-value: 2.6E-38
score: 132.0
IPR002016Haem peroxidasePROSITEPS50873PEROXIDASE_4coord: 122..336
score: 19.118118
NoneNo IPR availableGENE3D1.10.520.10coord: 110..331
e-value: 1.1E-64
score: 219.9
NoneNo IPR availableGENE3D1.10.420.10Peroxidase, domain 2coord: 222..324
e-value: 1.1E-64
score: 219.9
NoneNo IPR availablePIRSRPIRSR600823-3PIRSR600823-3coord: 109..256
e-value: 3.4E-20
score: 70.0
NoneNo IPR availablePANTHERPTHR31356:SF8L-ASCORBATE PEROXIDASE 6-RELATEDcoord: 15..335
NoneNo IPR availableCDDcd00314plant_peroxidase_likecoord: 107..329
e-value: 8.68986E-66
score: 206.236
IPR044831Heme-binding peroxidase Ccp1-likePANTHERPTHR31356THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATEDcoord: 15..335
IPR019793Peroxidases heam-ligand binding sitePROSITEPS00435PEROXIDASE_1coord: 244..254
IPR019794Peroxidase, active sitePROSITEPS00436PEROXIDASE_2coord: 122..133
IPR010255Haem peroxidase superfamilySUPERFAMILY48113Heme-dependent peroxidasescoord: 119..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g04550.1Moc08g04550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0034599 cellular response to oxidative stress
biological_process GO:0042744 hydrogen peroxide catabolic process
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0006979 response to oxidative stress
cellular_component GO:0005737 cytoplasm
molecular_function GO:0020037 heme binding
molecular_function GO:0016688 L-ascorbate peroxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004601 peroxidase activity