Moc08g04390 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g04390
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUPF0481 protein At3g47200-like
Locationchr8: 3201646 .. 3202878 (-)
RNA-Seq ExpressionMoc08g04390
SyntenyMoc08g04390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTGGACAAAATTGGCAACGTTCCGGCAGCTCTGTTCGAAATGAAACCAGAAGCTTATATTCCCCAATTCATCTTCATCGGCCATCCTGATCCTACTGTCGAGCATTTTGTAAGTGGAAATAATGATAAGATCATGAAAGGCTGCTTTGTCGGAAAGTTTTCTTCCGTTGCGAAAGTGGAGTTGAATGAGATTATAGGACGAGTCATAGGTTGGGAGCAAACAGCTCGTGATTGTTATTTGTTCATAGGTCGGAGAAAATTGGACACAGTCCAGTTTGTGAAGTTTCTAGTCATGGATGGTTGTTTCGTGGTCATGTATATGCTGGTTTCTGTGTTCCCGGAGTTTCAGGACATCGACACGTCATCGTTCTTTTGGAGATTCAACGATGCAGTATTCAGAGATCTGTTACTGTTTCAAAACCAACTTCCTTTCTTTCTTCTCCGGTCTCTATACGACCTGTGCGTCTCCAATAATCAAACTATACTTGGAAACACCTCTTTCATTCAACTTACTCATCAATTTTTTATTGACCGTGAAGGGATCGGTTATCTTGGAAAGGATTTTAGGGTAGAGGAAGACAAATTAGAAGTGAAGCATCTTATTCATTTTCTAAGTTGTTATATGAACTTTCCTCATCAAGACGATTCTTCAAAATCATTGGACGGCACCCCACTCATGGTTTCTGTTTGGCCACCCACTGCCACTGAGCTTTACGATTACGGCATTTCTTTCGAGAAGAAATCACATTATTCTCAAAAGATGTTTGATGAACGTACCGGCATTCTCAGAGTGCCTCACATCATAATAAATGAGACTTTCGAAAGCACGATGAGAAACATCATAGCTTACGAGTATACAATTCGCAAGAGTCCAGGCGTAAGCAACTTCTTGATGTTCATGCGTTTCTTGTTGAACTCCGACAACGATGTAAATTTGCTCATAAAGGAGGGGATTATCCACAACCATTTGGAAAGCGCAAAGGAAGTTACTAAGTTGTTCCAGGACCTTTGTAAGAACGTTGTGGCCGAAAGAAATTTGTACAACTATGAATGTCAGAAAATGAGAAAATACTGCAAGCACCGCCGCCATCGATGGATGGCTTCGTTGAAACACGACTATTTTAACACGCCGTGGGCTTTGATCTCCTTCATTGCTGCCGTCGTCCTGCTTTTACTCACTCTCATGCGAGCGGTGGTAGCTGTACTCTCCATGCCTAAGCCTAAGTGA

mRNA sequence

ATGGAGTTGGACAAAATTGGCAACGTTCCGGCAGCTCTGTTCGAAATGAAACCAGAAGCTTATATTCCCCAATTCATCTTCATCGGCCATCCTGATCCTACTGTCGAGCATTTTGTAAGTGGAAATAATGATAAGATCATGAAAGGCTGCTTTGTCGGAAAGTTTTCTTCCGTTGCGAAAGTGGAGTTGAATGAGATTATAGGACGAGTCATAGGTTGGGAGCAAACAGCTCGTGATTGTTATTTGTTCATAGGTCGGAGAAAATTGGACACAGTCCAGTTTGTGAAGTTTCTAGTCATGGATGGTTGTTTCGTGGTCATGTATATGCTGGTTTCTGTGTTCCCGGAGTTTCAGGACATCGACACGTCATCGTTCTTTTGGAGATTCAACGATGCAGTATTCAGAGATCTGTTACTGTTTCAAAACCAACTTCCTTTCTTTCTTCTCCGGTCTCTATACGACCTGTGCGTCTCCAATAATCAAACTATACTTGGAAACACCTCTTTCATTCAACTTACTCATCAATTTTTTATTGACCGTGAAGGGATCGGTTATCTTGGAAAGGATTTTAGGGTAGAGGAAGACAAATTAGAAGTGAAGCATCTTATTCATTTTCTAAGTTGTTATATGAACTTTCCTCATCAAGACGATTCTTCAAAATCATTGGACGGCACCCCACTCATGGTTTCTGTTTGGCCACCCACTGCCACTGAGCTTTACGATTACGGCATTTCTTTCGAGAAGAAATCACATTATTCTCAAAAGATGTTTGATGAACGTACCGGCATTCTCAGAGTGCCTCACATCATAATAAATGAGACTTTCGAAAGCACGATGAGAAACATCATAGCTTACGAGTATACAATTCGCAAGAGTCCAGGCGTAAGCAACTTCTTGATGTTCATGCGTTTCTTGTTGAACTCCGACAACGATGTAAATTTGCTCATAAAGGAGGGGATTATCCACAACCATTTGGAAAGCGCAAAGGAAGTTACTAAGTTGTTCCAGGACCTTTGTAAGAACGTTGTGGCCGAAAGAAATTTGTACAACTATGAATGTCAGAAAATGAGAAAATACTGCAAGCACCGCCGCCATCGATGGATGGCTTCGTTGAAACACGACTATTTTAACACGCCGTGGGCTTTGATCTCCTTCATTGCTGCCGTCGTCCTGCTTTTACTCACTCTCATGCGAGCGGTGGTAGCTGTACTCTCCATGCCTAAGCCTAAGTGA

Coding sequence (CDS)

ATGGAGTTGGACAAAATTGGCAACGTTCCGGCAGCTCTGTTCGAAATGAAACCAGAAGCTTATATTCCCCAATTCATCTTCATCGGCCATCCTGATCCTACTGTCGAGCATTTTGTAAGTGGAAATAATGATAAGATCATGAAAGGCTGCTTTGTCGGAAAGTTTTCTTCCGTTGCGAAAGTGGAGTTGAATGAGATTATAGGACGAGTCATAGGTTGGGAGCAAACAGCTCGTGATTGTTATTTGTTCATAGGTCGGAGAAAATTGGACACAGTCCAGTTTGTGAAGTTTCTAGTCATGGATGGTTGTTTCGTGGTCATGTATATGCTGGTTTCTGTGTTCCCGGAGTTTCAGGACATCGACACGTCATCGTTCTTTTGGAGATTCAACGATGCAGTATTCAGAGATCTGTTACTGTTTCAAAACCAACTTCCTTTCTTTCTTCTCCGGTCTCTATACGACCTGTGCGTCTCCAATAATCAAACTATACTTGGAAACACCTCTTTCATTCAACTTACTCATCAATTTTTTATTGACCGTGAAGGGATCGGTTATCTTGGAAAGGATTTTAGGGTAGAGGAAGACAAATTAGAAGTGAAGCATCTTATTCATTTTCTAAGTTGTTATATGAACTTTCCTCATCAAGACGATTCTTCAAAATCATTGGACGGCACCCCACTCATGGTTTCTGTTTGGCCACCCACTGCCACTGAGCTTTACGATTACGGCATTTCTTTCGAGAAGAAATCACATTATTCTCAAAAGATGTTTGATGAACGTACCGGCATTCTCAGAGTGCCTCACATCATAATAAATGAGACTTTCGAAAGCACGATGAGAAACATCATAGCTTACGAGTATACAATTCGCAAGAGTCCAGGCGTAAGCAACTTCTTGATGTTCATGCGTTTCTTGTTGAACTCCGACAACGATGTAAATTTGCTCATAAAGGAGGGGATTATCCACAACCATTTGGAAAGCGCAAAGGAAGTTACTAAGTTGTTCCAGGACCTTTGTAAGAACGTTGTGGCCGAAAGAAATTTGTACAACTATGAATGTCAGAAAATGAGAAAATACTGCAAGCACCGCCGCCATCGATGGATGGCTTCGTTGAAACACGACTATTTTAACACGCCGTGGGCTTTGATCTCCTTCATTGCTGCCGTCGTCCTGCTTTTACTCACTCTCATGCGAGCGGTGGTAGCTGTACTCTCCATGCCTAAGCCTAAGTGA

Protein sequence

MELDKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAKVELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDREGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELYDYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPKPK
Homology
BLAST of Moc08g04390 vs. NCBI nr
Match: XP_022131636.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 833.9 bits (2153), Expect = 5.8e-238
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MELDKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAK 60
           MELDKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAK
Sbjct: 1   MELDKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAK 60

Query: 61  VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI 120
           VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI
Sbjct: 61  VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI 120

Query: 121 DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDR 180
           DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDR
Sbjct: 121 DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDR 180

Query: 181 EGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELY 240
           EGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELY
Sbjct: 181 EGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELY 240

Query: 241 DYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLM 300
           DYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLM
Sbjct: 241 DYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLM 300

Query: 301 FMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYC 360
           FMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYC
Sbjct: 301 FMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYC 360

Query: 361 KHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPKPK 411
           KHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPKPK
Sbjct: 361 KHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPKPK 410

BLAST of Moc08g04390 vs. NCBI nr
Match: XP_022132033.1 (UPF0481 protein At3g47200-like [Momordica charantia] >XP_022132034.1 UPF0481 protein At3g47200-like [Momordica charantia] >XP_022132035.1 UPF0481 protein At3g47200-like [Momordica charantia] >XP_022132036.1 UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 496.9 bits (1278), Expect = 1.7e-136
Identity = 275/414 (66.43%), Postives = 314/414 (75.85%), Query Frame = 0

Query: 4   DKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKI---MKGCFVGKFSSVAK 63
           D I  VP AL+   P A+IPQFI IG   P      S   D I   MK  F   F+S  +
Sbjct: 23  DGIFRVPLALWRANPNAFIPQFIPIG---PVHGFHNSYGYDPIIRKMKFYFFRNFAS-TE 82

Query: 64  VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI 123
           VELN+I+  VIG E+ A + Y    R  +   +F++ L++D CFVVMY+L SV P FQD 
Sbjct: 83  VELNKIVRLVIGCEKRATEFYKIDIR--MGRAEFLESLILDCCFVVMYILSSVVPLFQDF 142

Query: 124 DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNN-QTILGNTSFIQLTHQFFID 183
           + SSF WRFNDA+FRD++LF+NQLPFFLL+SLY+LC  NN ++ILG  SFIQLTH+FF+ 
Sbjct: 143 EMSSFSWRFNDAIFRDMVLFENQLPFFLLQSLYELCAFNNYRSILGEFSFIQLTHKFFMA 202

Query: 184 REGIGYLGKDFRV-EEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMV----SVWPP 243
           REGIGYLGKDFRV  EDKLEV H +HFLS YMN         SLD T   V    ++WPP
Sbjct: 203 REGIGYLGKDFRVLHEDKLEVNHFVHFLSYYMN---------SLDHTRWRVGPSLAIWPP 262

Query: 244 TATELYDYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPG 303
           TATELYDYGISFEKKSHYSQKMFDER GILR+PHIIINETFES +RNIIA+E+  RK   
Sbjct: 263 TATELYDYGISFEKKSHYSQKMFDERAGILRLPHIIINETFESMIRNIIAFEHCHRKRQV 322

Query: 304 VSNFLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQ 363
           VSNF +FMRFLLNSDNDV LLIKEGIIHNHLES K VTKLF DLC+NVV E NLYNYEC+
Sbjct: 323 VSNFAIFMRFLLNSDNDVILLIKEGIIHNHLESTKGVTKLFHDLCENVVVETNLYNYECK 382

Query: 364 KMRKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           +MR+Y KHRRHRWMASLK DYFNTPWALISFIAAVVLLLLTLM+ VVAVLSMPK
Sbjct: 383 RMREYGKHRRHRWMASLKRDYFNTPWALISFIAAVVLLLLTLMQTVVAVLSMPK 421

BLAST of Moc08g04390 vs. NCBI nr
Match: KAA0064930.1 (UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 350.9 bits (899), Expect = 1.5e-92
Identity = 197/409 (48.17%), Postives = 272/409 (66.50%), Query Frame = 0

Query: 9   VPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNND---KIMKGCFVGKFSSVAKVELNE 68
           VP  L    P+AY PQ I IG       H+    ND   K  KG +V  F +VAK+  NE
Sbjct: 55  VPKLLRNGNPKAYSPQVISIG-----PLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNE 114

Query: 69  IIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSF 128
           +I + + WE+ AR+ Y  +   K++  +F++ L+ D CFVVMY++ S+  EF+D+DT SF
Sbjct: 115 MINKFLCWEERARNYY--VETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDT-SF 174

Query: 129 FWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFID-REGIG 188
            WRF++ +F+DLLL +NQLPFFLL  LY+LC +  Q  L + SFI+L   +F + REG+ 
Sbjct: 175 LWRFSNGIFKDLLLLENQLPFFLLHHLYNLC-AFAQPSLKDISFIELLRGYFTEVREGMS 234

Query: 189 YLGKDFRVEEDKLEVKHLIHFLSCYM----NFPHQDDSSKSLDGTPLMVSVWPPTATELY 248
           Y+ + + ++ D  EV HL+ FL  ++    + PH  D S         +S WP TATEL+
Sbjct: 235 YVNEGY-LDIDANEVNHLVDFLRIHLTQPRHIPHFLDFS-----VDDFLSSWPLTATELH 294

Query: 249 DYGISFEKKSHYSQKM-FDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFL 308
           D GISF  +      + F ER G+L++P III+++FE   RN+IAYEY   KS  VSNF 
Sbjct: 295 DCGISFHGQKRCMMNVNFRERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKDVSNFG 354

Query: 309 MFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKY 368
           MFM FL+N++ DV+LL+ +GII NHL S +E+  LF DLCKN++ ERNLY+ EC+KM++Y
Sbjct: 355 MFMHFLINTNKDVSLLVDDGIIQNHLGSTREIVVLFNDLCKNIIVERNLYSIECRKMKEY 414

Query: 369 CKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           CKHRRHRWM SLK DYF TPWA ISF+AAV+LLLLTL++ VVA +++ K
Sbjct: 415 CKHRRHRWMTSLKRDYFGTPWAFISFVAAVLLLLLTLLQTVVAFVALYK 448

BLAST of Moc08g04390 vs. NCBI nr
Match: XP_008445209.1 (PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 350.1 bits (897), Expect = 2.5e-92
Identity = 197/409 (48.17%), Postives = 272/409 (66.50%), Query Frame = 0

Query: 9   VPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNND---KIMKGCFVGKFSSVAKVELNE 68
           VP  L    P+AY PQ I IG       H+    ND   K  KG +V  F +VAK+  NE
Sbjct: 55  VPKLLRNGNPKAYSPQVISIG-----PLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNE 114

Query: 69  IIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSF 128
           +I + + WE+ AR+ Y  +   K++  +F++ L+ D CFVVMY++ S+  EF+D+DT SF
Sbjct: 115 MINKFLCWEERARNYY--VETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDT-SF 174

Query: 129 FWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFID-REGIG 188
            WRF++ +F+DLLL +NQLPFFLL  LY+LC +  Q  L + SFI+L   +F + REG+ 
Sbjct: 175 LWRFSNGIFKDLLLLENQLPFFLLHHLYNLC-AFAQPSLKDISFIELLRGYFTEVREGMS 234

Query: 189 YLGKDFRVEEDKLEVKHLIHFLSCYM----NFPHQDDSSKSLDGTPLMVSVWPPTATELY 248
           Y+ + + ++ D  EV HL+ FL  ++    + PH  D S         +S WP TATEL+
Sbjct: 235 YVNEGY-LDIDANEVNHLVDFLRIHLTQPRHIPHFLDFS-----VDDFLSSWPLTATELH 294

Query: 249 DYGISFEKKSHYSQKM-FDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFL 308
           D GISF  +      + F ER G+L++P III+++FE   RN+IAYEY   KS  VSNF 
Sbjct: 295 DCGISFHGQKRCMMNVNFRERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKDVSNFG 354

Query: 309 MFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKY 368
           MFM FL+N++ DV+LL+ +GII NHL S +E+  LF DLCKN++ ERNLY+ EC+KM++Y
Sbjct: 355 MFMHFLINTNKDVSLLVDDGIIQNHLGSTREIVVLFNDLCKNIMVERNLYSIECRKMKEY 414

Query: 369 CKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           CKHRRHRWM SLK DYF TPWA ISF+AAV+LLLLTL++ VVA +++ K
Sbjct: 415 CKHRRHRWMTSLKRDYFGTPWAFISFVAAVLLLLLTLLQTVVAFVALYK 448

BLAST of Moc08g04390 vs. NCBI nr
Match: XP_004138858.1 (UPF0481 protein At3g47200 [Cucumis sativus] >KGN62944.1 hypothetical protein Csa_022605 [Cucumis sativus])

HSP 1 Score: 339.3 bits (869), Expect = 4.5e-89
Identity = 193/404 (47.77%), Postives = 262/404 (64.85%), Query Frame = 0

Query: 9   VPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNND--KIMKGCFVGKFSSVAKVELNEI 68
           VP  L +  P+AY PQ I IG       H+    ND  K  KG +V  F +VAK++ NE+
Sbjct: 56  VPKLLRKGNPKAYSPQVISIG-----PLHYYRTQNDLIKEKKGSYVLNFLTVAKLDWNEM 115

Query: 69  IGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSFF 128
           I + + WE+ AR+ Y+     K D  +F++ L+ D CFVVMY++ S+  EF+D+DT SF 
Sbjct: 116 IKKFLSWEERARNYYVETIEMKRD--EFIQLLIYDSCFVVMYVIGSMVAEFRDLDT-SFL 175

Query: 129 WRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFID-REGIGY 188
           WRF++ +F+DLLL +NQLPFFLL  LY+LC S  Q  L + SFI+L   +F   REG+ Y
Sbjct: 176 WRFSNGIFKDLLLLENQLPFFLLNHLYNLCAS-AQPSLKDISFIELLRGYFSKVREGMSY 235

Query: 189 LGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELYDYGIS 248
           + K+   + D   V HL+ FL  ++  P        L     + S WP TATEL++ GIS
Sbjct: 236 V-KEGYFDIDASAVNHLVDFLRIHLTQPRHIPHFFGLSVDDFL-SSWPLTATELHECGIS 295

Query: 249 FE-KKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLMFMRF 308
           F  +K       F ER G+L++P III+++FE   RN+IAYEY   KS   SNF MFM F
Sbjct: 296 FHGQKKCMMNVSFKERRGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKDASNFGMFMHF 355

Query: 309 LLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYCKHRR 368
           L+N++ DV+LL+ +GII N L S KE+  LF DLCKN++ ERN Y+  C +M++YCKHRR
Sbjct: 356 LINTNEDVSLLVDDGIIQNQLGSTKEIVVLFSDLCKNIMIERNFYSIACWRMKEYCKHRR 415

Query: 369 HRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           HRWM SLK DYF TPWA ISF+AAV+LLLLTL++ VVA +++ K
Sbjct: 416 HRWMTSLKRDYFGTPWAFISFVAAVLLLLLTLLQTVVAFIALYK 448

BLAST of Moc08g04390 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.4e-21
Identity = 111/433 (25.64%), Postives = 203/433 (46.88%), Query Frame = 0

Query: 6   IGNVPAALFEMKPEAYIPQFIFIG--HPDPTVEHFVSGNNDKIMKGCFVGKFSSVAKVEL 65
           I  VP +   + P+AY P+ + IG  H        +  +  ++++  F+ + +    VE 
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQ-LFLDE-AKKKDVEE 107

Query: 66  NEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKF-LVMDGCFVVM-YMLVSVFPEFQDID 125
           N ++  V+  E   R  Y      +L T   + F +V+DGCF++M ++++S   E  +  
Sbjct: 108 NVLVKAVVDLEDKIRKSY----SEELKTGHDLMFMMVLDGCFILMVFLIMSGNIELSEDP 167

Query: 126 TSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFF---I 185
             S  W  + ++  DLLL +NQ+PFF+L++LY         I  ++   ++   FF   I
Sbjct: 168 IFSIPWLLS-SIQSDLLLLENQVPFFVLQTLY-----VGSKIGVSSDLNRIAFHFFKNPI 227

Query: 186 DREGIGYLGKDFRVEEDKLEVKHLIHFL----------SCYMNFPH--------QDDSSK 245
           D+EG       +  +    + KHL+  +          S   + PH        +  +  
Sbjct: 228 DKEG------SYWEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLHEGKSGNVP 287

Query: 246 SLD--GTPLMVSVWPPTATELYDYGISFEKKSHYSQKMFDER--TGILRVPHIIINETFE 305
           S+D    PL++S     A  L   GI F  +      + + R     L++P +  +    
Sbjct: 288 SVDSKAVPLILS-----AKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFIS 347

Query: 306 STMRNIIAYE-YTIRKSPGVSNFLMFMRFLLNSDNDVNLLIKEG-IIHNHLESAKEVTKL 365
           S   N +A+E +    S  ++ +++FM  LLN++ DV  L  +  II NH  S  EV++ 
Sbjct: 348 SFFLNCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEF 407

Query: 366 FQDLCKNVVAE--RNLYNYECQKMRKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLL 406
           F+ + K+VV E   +  N   + + +Y K   +   A  +H +F +PW  +S  A + ++
Sbjct: 408 FKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVI 457

BLAST of Moc08g04390 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 5.6e-10
Identity = 47/188 (25.00%), Postives = 100/188 (53.19%), Query Frame = 0

Query: 222 LDGTPLMVSVWPPTATELYDYGISFEKKSH--YSQKMFDERTGILRVPHIIINETFESTM 281
           ++  PL+  +  P+ ++L+  G+ F+  +H   S   FD  +G   +P I ++   E+ +
Sbjct: 334 IEKPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVL 393

Query: 282 RNIIAYEYTIRKSPGV-SNFLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDL 341
           RN++AYE T    P V + +   +  +++S+ DV LL ++G++ + L+S +E  +++  +
Sbjct: 394 RNLVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGM 453

Query: 342 CKNV-VAERNLYNYECQKMRKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLM 401
            K+V + +    +   + + +Y   R    +  L   Y    W +++F+AAV+LL+L  +
Sbjct: 454 SKSVRLTKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSL 513

Query: 402 RAVVAVLS 406
           +    V S
Sbjct: 514 QLFSLVFS 521

BLAST of Moc08g04390 vs. ExPASy TrEMBL
Match: A0A6J1BQ21 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004765 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 2.8e-238
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MELDKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAK 60
           MELDKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAK
Sbjct: 1   MELDKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAK 60

Query: 61  VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI 120
           VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI
Sbjct: 61  VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI 120

Query: 121 DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDR 180
           DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDR
Sbjct: 121 DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDR 180

Query: 181 EGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELY 240
           EGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELY
Sbjct: 181 EGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELY 240

Query: 241 DYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLM 300
           DYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLM
Sbjct: 241 DYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLM 300

Query: 301 FMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYC 360
           FMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYC
Sbjct: 301 FMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYC 360

Query: 361 KHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPKPK 411
           KHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPKPK
Sbjct: 361 KHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPKPK 410

BLAST of Moc08g04390 vs. ExPASy TrEMBL
Match: A0A6J1BR42 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005004 PE=4 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 8.1e-137
Identity = 275/414 (66.43%), Postives = 314/414 (75.85%), Query Frame = 0

Query: 4   DKIGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKI---MKGCFVGKFSSVAK 63
           D I  VP AL+   P A+IPQFI IG   P      S   D I   MK  F   F+S  +
Sbjct: 23  DGIFRVPLALWRANPNAFIPQFIPIG---PVHGFHNSYGYDPIIRKMKFYFFRNFAS-TE 82

Query: 64  VELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDI 123
           VELN+I+  VIG E+ A + Y    R  +   +F++ L++D CFVVMY+L SV P FQD 
Sbjct: 83  VELNKIVRLVIGCEKRATEFYKIDIR--MGRAEFLESLILDCCFVVMYILSSVVPLFQDF 142

Query: 124 DTSSFFWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNN-QTILGNTSFIQLTHQFFID 183
           + SSF WRFNDA+FRD++LF+NQLPFFLL+SLY+LC  NN ++ILG  SFIQLTH+FF+ 
Sbjct: 143 EMSSFSWRFNDAIFRDMVLFENQLPFFLLQSLYELCAFNNYRSILGEFSFIQLTHKFFMA 202

Query: 184 REGIGYLGKDFRV-EEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMV----SVWPP 243
           REGIGYLGKDFRV  EDKLEV H +HFLS YMN         SLD T   V    ++WPP
Sbjct: 203 REGIGYLGKDFRVLHEDKLEVNHFVHFLSYYMN---------SLDHTRWRVGPSLAIWPP 262

Query: 244 TATELYDYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPG 303
           TATELYDYGISFEKKSHYSQKMFDER GILR+PHIIINETFES +RNIIA+E+  RK   
Sbjct: 263 TATELYDYGISFEKKSHYSQKMFDERAGILRLPHIIINETFESMIRNIIAFEHCHRKRQV 322

Query: 304 VSNFLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQ 363
           VSNF +FMRFLLNSDNDV LLIKEGIIHNHLES K VTKLF DLC+NVV E NLYNYEC+
Sbjct: 323 VSNFAIFMRFLLNSDNDVILLIKEGIIHNHLESTKGVTKLFHDLCENVVVETNLYNYECK 382

Query: 364 KMRKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           +MR+Y KHRRHRWMASLK DYFNTPWALISFIAAVVLLLLTLM+ VVAVLSMPK
Sbjct: 383 RMREYGKHRRHRWMASLKRDYFNTPWALISFIAAVVLLLLTLMQTVVAVLSMPK 421

BLAST of Moc08g04390 vs. ExPASy TrEMBL
Match: A0A5A7VCL1 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002480 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 7.2e-93
Identity = 197/409 (48.17%), Postives = 272/409 (66.50%), Query Frame = 0

Query: 9   VPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNND---KIMKGCFVGKFSSVAKVELNE 68
           VP  L    P+AY PQ I IG       H+    ND   K  KG +V  F +VAK+  NE
Sbjct: 55  VPKLLRNGNPKAYSPQVISIG-----PLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNE 114

Query: 69  IIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSF 128
           +I + + WE+ AR+ Y  +   K++  +F++ L+ D CFVVMY++ S+  EF+D+DT SF
Sbjct: 115 MINKFLCWEERARNYY--VETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDT-SF 174

Query: 129 FWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFID-REGIG 188
            WRF++ +F+DLLL +NQLPFFLL  LY+LC +  Q  L + SFI+L   +F + REG+ 
Sbjct: 175 LWRFSNGIFKDLLLLENQLPFFLLHHLYNLC-AFAQPSLKDISFIELLRGYFTEVREGMS 234

Query: 189 YLGKDFRVEEDKLEVKHLIHFLSCYM----NFPHQDDSSKSLDGTPLMVSVWPPTATELY 248
           Y+ + + ++ D  EV HL+ FL  ++    + PH  D S         +S WP TATEL+
Sbjct: 235 YVNEGY-LDIDANEVNHLVDFLRIHLTQPRHIPHFLDFS-----VDDFLSSWPLTATELH 294

Query: 249 DYGISFEKKSHYSQKM-FDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFL 308
           D GISF  +      + F ER G+L++P III+++FE   RN+IAYEY   KS  VSNF 
Sbjct: 295 DCGISFHGQKRCMMNVNFRERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKDVSNFG 354

Query: 309 MFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKY 368
           MFM FL+N++ DV+LL+ +GII NHL S +E+  LF DLCKN++ ERNLY+ EC+KM++Y
Sbjct: 355 MFMHFLINTNKDVSLLVDDGIIQNHLGSTREIVVLFNDLCKNIIVERNLYSIECRKMKEY 414

Query: 369 CKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           CKHRRHRWM SLK DYF TPWA ISF+AAV+LLLLTL++ VVA +++ K
Sbjct: 415 CKHRRHRWMTSLKRDYFGTPWAFISFVAAVLLLLLTLLQTVVAFVALYK 448

BLAST of Moc08g04390 vs. ExPASy TrEMBL
Match: A0A1S3BD00 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488308 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 1.2e-92
Identity = 197/409 (48.17%), Postives = 272/409 (66.50%), Query Frame = 0

Query: 9   VPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNND---KIMKGCFVGKFSSVAKVELNE 68
           VP  L    P+AY PQ I IG       H+    ND   K  KG +V  F +VAK+  NE
Sbjct: 55  VPKLLRNGNPKAYSPQVISIG-----PLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNE 114

Query: 69  IIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSF 128
           +I + + WE+ AR+ Y  +   K++  +F++ L+ D CFVVMY++ S+  EF+D+DT SF
Sbjct: 115 MINKFLCWEERARNYY--VETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDT-SF 174

Query: 129 FWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFID-REGIG 188
            WRF++ +F+DLLL +NQLPFFLL  LY+LC +  Q  L + SFI+L   +F + REG+ 
Sbjct: 175 LWRFSNGIFKDLLLLENQLPFFLLHHLYNLC-AFAQPSLKDISFIELLRGYFTEVREGMS 234

Query: 189 YLGKDFRVEEDKLEVKHLIHFLSCYM----NFPHQDDSSKSLDGTPLMVSVWPPTATELY 248
           Y+ + + ++ D  EV HL+ FL  ++    + PH  D S         +S WP TATEL+
Sbjct: 235 YVNEGY-LDIDANEVNHLVDFLRIHLTQPRHIPHFLDFS-----VDDFLSSWPLTATELH 294

Query: 249 DYGISFEKKSHYSQKM-FDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFL 308
           D GISF  +      + F ER G+L++P III+++FE   RN+IAYEY   KS  VSNF 
Sbjct: 295 DCGISFHGQKRCMMNVNFRERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKDVSNFG 354

Query: 309 MFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKY 368
           MFM FL+N++ DV+LL+ +GII NHL S +E+  LF DLCKN++ ERNLY+ EC+KM++Y
Sbjct: 355 MFMHFLINTNKDVSLLVDDGIIQNHLGSTREIVVLFNDLCKNIMVERNLYSIECRKMKEY 414

Query: 369 CKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           CKHRRHRWM SLK DYF TPWA ISF+AAV+LLLLTL++ VVA +++ K
Sbjct: 415 CKHRRHRWMTSLKRDYFGTPWAFISFVAAVLLLLLTLLQTVVAFVALYK 448

BLAST of Moc08g04390 vs. ExPASy TrEMBL
Match: A0A0A0LPK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G381700 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.2e-89
Identity = 193/404 (47.77%), Postives = 262/404 (64.85%), Query Frame = 0

Query: 9   VPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNND--KIMKGCFVGKFSSVAKVELNEI 68
           VP  L +  P+AY PQ I IG       H+    ND  K  KG +V  F +VAK++ NE+
Sbjct: 56  VPKLLRKGNPKAYSPQVISIG-----PLHYYRTQNDLIKEKKGSYVLNFLTVAKLDWNEM 115

Query: 69  IGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSFF 128
           I + + WE+ AR+ Y+     K D  +F++ L+ D CFVVMY++ S+  EF+D+DT SF 
Sbjct: 116 IKKFLSWEERARNYYVETIEMKRD--EFIQLLIYDSCFVVMYVIGSMVAEFRDLDT-SFL 175

Query: 129 WRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFID-REGIGY 188
           WRF++ +F+DLLL +NQLPFFLL  LY+LC S  Q  L + SFI+L   +F   REG+ Y
Sbjct: 176 WRFSNGIFKDLLLLENQLPFFLLNHLYNLCAS-AQPSLKDISFIELLRGYFSKVREGMSY 235

Query: 189 LGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPPTATELYDYGIS 248
           + K+   + D   V HL+ FL  ++  P        L     + S WP TATEL++ GIS
Sbjct: 236 V-KEGYFDIDASAVNHLVDFLRIHLTQPRHIPHFFGLSVDDFL-SSWPLTATELHECGIS 295

Query: 249 FE-KKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSNFLMFMRF 308
           F  +K       F ER G+L++P III+++FE   RN+IAYEY   KS   SNF MFM F
Sbjct: 296 FHGQKKCMMNVSFKERRGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKDASNFGMFMHF 355

Query: 309 LLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLYNYECQKMRKYCKHRR 368
           L+N++ DV+LL+ +GII N L S KE+  LF DLCKN++ ERN Y+  C +M++YCKHRR
Sbjct: 356 LINTNEDVSLLVDDGIIQNQLGSTKEIVVLFSDLCKNIMIERNFYSIACWRMKEYCKHRR 415

Query: 369 HRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 409
           HRWM SLK DYF TPWA ISF+AAV+LLLLTL++ VVA +++ K
Sbjct: 416 HRWMTSLKRDYFGTPWAFISFVAAVLLLLLTLLQTVVAFIALYK 448

BLAST of Moc08g04390 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 165.6 bits (418), Expect = 8.3e-41
Identity = 127/410 (30.98%), Postives = 213/410 (51.95%), Query Frame = 0

Query: 6   IGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAKVELNE 65
           I  VP  L  + P+AY P+ +  G      E   +  + K     ++  F       L +
Sbjct: 295 IYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQKYR---YLLSFIPRTNSSLED 354

Query: 66  IIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSF 125
           ++     WEQ AR CY      KL + +FV+ LV+DG F+V  +L S +P  +  +   F
Sbjct: 355 LVRLARTWEQNARSCY--AEDVKLHSDEFVEMLVVDGSFLVELLLRSHYPRLRGENDRIF 414

Query: 126 --FWRFNDAVFRDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFFIDREGI 185
                  D V RD++L +NQLPFF+++ ++ L ++  Q   G  S IQL  + F      
Sbjct: 415 GNSMMITD-VCRDMILIENQLPFFVVKEIFLLLLNYYQQ--GTPSIIQLAQRHF------ 474

Query: 186 GYLGKDFRVEEDKL--EVKHLIHFL-SCYM-NFPHQDDSSKSLDGTPLMVSVWPPTATEL 245
            Y     R++++K   E +H +  L SCY+  FP +      L+ T + V    P ATEL
Sbjct: 475 SYFLS--RIDDEKFITEPEHFVDLLRSCYLPQFPIK------LEYTTVKVDN-APEATEL 534

Query: 246 YDYGISFEKKSHYSQKMFD--ERTGILRVPHIIINETFESTMRNIIAYEYTIRKSPGVSN 305
           +  G+ F K +  S  + D     G+L++P I++++  ES  +NII +E     +    +
Sbjct: 535 HTAGVRF-KPAETSSCLLDISFADGVLKIPTIVVDDLTESLYKNIIGFEQCRCSNKNFLD 594

Query: 306 FLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERNLY-NYECQKM 365
           ++M +   + S  D +LLI  GII N+L ++ +V+ LF  + K V+ +R  Y +   + +
Sbjct: 595 YIMLLGCFIKSPTDADLLIHSGIIVNYLGNSVDVSNLFNSISKEVIYDRRFYFSMLSENL 654

Query: 366 RKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSM 407
           + YC    +RW A L+ DYF+ PWA+ S  AA++LLLLT +++V ++L++
Sbjct: 655 QAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLTFIQSVCSILAL 680

BLAST of Moc08g04390 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 126.7 bits (317), Expect = 4.2e-29
Identity = 121/421 (28.74%), Postives = 197/421 (46.79%), Query Frame = 0

Query: 6   IGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAKVELNE 65
           I  VP  L E   ++Y+PQ + IG      +H +     K      V    + AK ++  
Sbjct: 106 IYRVPPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRA---VNMVMARAKHDIEM 165

Query: 66  IIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSF 125
            I  +   E+ AR CY   G   ++  +F++ LV+DG F++  +       FQ+I  +  
Sbjct: 166 YIDAMKELEEKARACYQ--GPINMNRNEFIEMLVLDGVFII-EIFKGTSEGFQEIGYAP- 225

Query: 126 FWRFNDAVF----------RDLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQ 185
               ND VF          RD+++ +NQLP+ +L+ L  L       +L   + +QL   
Sbjct: 226 ----NDPVFGMRGLMQSIRRDMVMLENQLPWSVLKGLLQL---QRPDVLDKVN-VQLFQP 285

Query: 186 FFIDREGIGYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGTPLMVSVWPP- 245
           FF          +      + L  +  +H L        Q  SS + D    MV+  P  
Sbjct: 286 FF----------QPLLPTREVLTEEGGLHCLDVLRRGLLQ--SSGTSDEDMSMVNKQPQQ 345

Query: 246 ---TATELYDYGISFEKKSHYSQKMFDERTGILRVPHIIINETFESTMRNIIAYEYT-IR 305
                TEL + G+ F +K        + + G L++P ++I++  +S   N+IA+E   I+
Sbjct: 346 LIHCVTELRNAGVEFMRKETGHFWDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFEQCHIK 405

Query: 306 KSPGVSNFLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQDLCKNVVAERN--L 365
            S  ++++++FM  L+NS  DV+ L   GII N L S  EV+ LF  L K V+ + N   
Sbjct: 406 SSKKITSYIIFMDNLINSSEDVSYLHHYGIIENWLGSDSEVSDLFNGLGKEVIFDPNDGY 465

Query: 366 YNYECQKMRKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLLLLTLMRAVVAVLSMPK 410
            +    ++  Y + + +   A+L+H YFN PWA  SFIAAV LL+ T  ++  AV +  K
Sbjct: 466 LSALTGEVNIYYRRKWNYLKATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFAVFAYFK 499

BLAST of Moc08g04390 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 124.8 bits (312), Expect = 1.6e-28
Identity = 123/437 (28.15%), Postives = 194/437 (44.39%), Query Frame = 0

Query: 6   IGNVPAALFEMKPEAYIPQFIFIGHPDPTVEHFVSGNNDKIMKGCFVGKFSSVAKVELNE 65
           I  VP  L E   ++Y PQ + +G      +   S +  K      V +        +  
Sbjct: 105 IYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRSMDRHKWRA---VNRVLKRTNQGIKM 164

Query: 66  IIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQDIDTSSF 125
            I  +   E+ AR CY   G   L + +F++ LV+DGCF V+ +       F ++     
Sbjct: 165 YIDAMRELEEKARACY--EGPLSLSSNEFIEMLVLDGCF-VLELFRGAVEGFTELG---- 224

Query: 126 FWRFNDAVF----------RDLLLFQNQLPFFLLRSLYDLCV-SNNQTILGNTSFIQLTH 185
            +  ND VF          RD+++ +NQLP F+L  L +L + + NQT L     I+   
Sbjct: 225 -YARNDPVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFD 284

Query: 186 QFFIDREGIGYLGKDFRVEEDKLEVKHLIHF-----LSCYMNFPHQDDSSKSLDGTPLMV 245
                 E +   G+  ++E      K    F     L C   F      S       L  
Sbjct: 285 PLMPTDEPLTKSGQS-KLENSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTR 344

Query: 246 SVWPPT--------------ATELYDYGISFEKKSHYSQKMFDERTGILRVPHIIINETF 305
             W                  TEL + GI F ++          + G L +P ++I++  
Sbjct: 345 KRWSRNTRVADKRRQQLIHCVTELKEAGIKFRRRKTDRFWDMQFKNGYLEIPRLLIHDGT 404

Query: 306 ESTMRNIIAYEYT-IRKSPGVSNFLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKL 365
           +S   N+IA+E   I  S  ++++++FM  L++S  DV+ L   GII + L S  EV  L
Sbjct: 405 KSLFLNLIAFEQCHIDSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEVADL 464

Query: 366 FQDLCKNVV--AERNLYNYECQKMRKYCKHRRHRWMASLKHDYFNTPWALISFIAAVVLL 410
           F  LC+ VV   E +  +    ++ +Y  H+ + W A+LKH YFN PWA++SF AAV+LL
Sbjct: 465 FNRLCQEVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILL 524

BLAST of Moc08g04390 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 123.2 bits (308), Expect = 4.7e-28
Identity = 126/435 (28.97%), Postives = 194/435 (44.60%), Query Frame = 0

Query: 6   IGNVPAALFEMKPEAYIPQFIFIG-------HPDPTVEHFVSGNNDKIMKGCFVGKFSSV 65
           I  VP  L E   ++Y+PQ + IG       H  P   H          K   V    + 
Sbjct: 92  IYRVPFYLQENDKKSYLPQTVSIGPYHHGKVHLRPMERH----------KWRAVNMIMAR 151

Query: 66  AKVELNEIIGRVIGWEQTARDCYLFIGRRKLDTVQFVKFLVMDGCFVVMYMLVSVFPEFQ 125
            K  +   I  +   E+ AR CY      K ++ +F + LV+DGCF V+ +       FQ
Sbjct: 152 TKHNIEMYIDAMKELEEEARACYQGPIDMK-NSNEFTEMLVLDGCF-VLELFKGTIQGFQ 211

Query: 126 DIDTSSFFWRFNDAVF----------RDLLLFQNQLPFFLLRSLYDLCVSN-NQTILGNT 185
            I      +  ND VF          RD+++ +NQLP F+L  L  L     NQT +   
Sbjct: 212 KIG-----YARNDPVFAKRGLMHSIQRDMIMLENQLPLFVLDRLLGLQTGTPNQTGIVAE 271

Query: 186 SFIQLTHQFFIDREGI--GYLGKDFRVEEDKLEVKHLIHFLSCYMNFPHQDDSSKSLDGT 245
             ++         E +       D + + D+L     +H L  +     Q  S  +  GT
Sbjct: 272 VAVRFFKTLMPTSEVLTKSERSLDSQEKSDELGDNGGLHCLDVFHRSLIQ-SSETTNQGT 331

Query: 246 PL----MVSVWPP---TATELYDYGISFEKKSHYSQKMFDERTGILRVPHIIINETFEST 305
           P     MV          TEL   G++F +K        + + G L++P ++I++  +S 
Sbjct: 332 PYEDMSMVEKQQQLIHCVTELRGAGVNFMRKETGQLWDIEFKNGYLKIPKLLIHDGTKSL 391

Query: 306 MRNIIAYEYT-IRKSPGVSNFLMFMRFLLNSDNDVNLLIKEGIIHNHLESAKEVTKLFQD 365
             N+IA+E    + S  ++++++FM  L+NS  DV+ L  +GII + L S  EV  LF  
Sbjct: 392 FSNLIAFEQCHTQSSNNITSYIIFMDNLINSSQDVSYLHHDGIIEHWLGSDSEVADLFNR 451

Query: 366 LCKNVVAERNLYNYECQKMRKYCKHRRHRW---MASLKHDYFNTPWALISFIAAVVLLLL 410
           LCK V+ +     Y  Q  R+  ++   +W    A+L+  YFN PWA  SF AAV+LL L
Sbjct: 452 LCKEVIFDPK-DGYLSQLSREVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFL 507

BLAST of Moc08g04390 vs. TAIR 10
Match: AT5G22550.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 119.0 bits (297), Expect = 8.9e-27
Identity = 115/457 (25.16%), Postives = 214/457 (46.83%), Query Frame = 0

Query: 6   IGNVPAALFEMKPEAYIPQFIFIG--HPDPTVEHF-VSGNNDKIMKGCFVGKFSSVAKVE 65
           I  +P  L ++  +AY P+ + IG  H     +H  +   + K     FV K +    V 
Sbjct: 46  IYRIPHTLKQVNDKAYAPKIVSIGPYHHSSDKQHLKMIEEHKKRYLEMFVSK-TKENGVY 105

Query: 66  LNEIIGRVIGWEQTARDCY---LFIGRRKLDTVQFVKFLVMDGCFVVM-YMLVSVFPEFQ 125
           L  ++  V G EQ  RD Y   L   ++KL     +K +++DGCF++M +++VS   E+ 
Sbjct: 106 LIHLVDLVSGLEQKIRDSYSENLEFSQQKL-----IKVMLLDGCFILMLFLVVSQKIEYT 165

Query: 126 DIDTSSFFWRFNDAVFR-DLLLFQNQLPFFLLRSLYDLCVSNNQTILGNTSFIQLTHQFF 185
           ++    F  R+     R DLLL +NQ+P FLL+ L +        +  +TS   L  +FF
Sbjct: 166 NLKDPIFKLRWILPTLRSDLLLLENQVPLFLLKVLLE-----TSKLAPSTSLNMLAFKFF 225

Query: 186 IDREGIGYLGKDFRVEEDKLEVKHLIHFL---------------SCYMN-------FPHQ 245
              +      + F  + + L  KHL+  +                C +N       +   
Sbjct: 226 ---DYSIKKPEGFWEKHNNLRAKHLLDLIRKTFIPAPPPSTTPRQCCINIFNGPREYSRT 285

Query: 246 DDS----------SKSLDGTPLMVSVWPP---------TATELYDYGISFEKKSHYSQKM 305
           + S          SK + G        PP         +A +L   GI F +K +    +
Sbjct: 286 ETSKNICLGKISCSKEITGAQTSSPPPPPRRPFLGLIVSARKLRLRGIKFMRKENVETPL 345

Query: 306 -FDERTGILRVPHIIINETFESTMRNIIAYE-YTIRKSPGVSNFLMFMRFLLNSDNDVNL 365
               ++G++ +P ++ ++   + + N +A+E + +  S  +++F++FM  L+N+++D   
Sbjct: 346 DISFKSGLVEIPLLVFDDFISNLLINCVAFEQFNMSCSTEITSFVIFMGCLINTEDDATF 405

Query: 366 LIKEGIIHNHLESAKEVTKLFQDLCKNV--VAERNLYNYECQKMRKYCKHRRHRWMASLK 410
           LI++GI+ N+  + +EV+  F+++ K++     ++  +   + + +Y     H   A  K
Sbjct: 406 LIEKGILENYFGTGEEVSLFFKNIGKDISFSISKSFLSNVFEGVNEYTSQGYHVHWAGFK 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131636.15.8e-238100.00UPF0481 protein At3g47200-like [Momordica charantia][more]
XP_022132033.11.7e-13666.43UPF0481 protein At3g47200-like [Momordica charantia] >XP_022132034.1 UPF0481 pro... [more]
KAA0064930.11.5e-9248.17UPF0481 protein [Cucumis melo var. makuwa][more]
XP_008445209.12.5e-9248.17PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo][more]
XP_004138858.14.5e-8947.77UPF0481 protein At3g47200 [Cucumis sativus] >KGN62944.1 hypothetical protein Csa... [more]
Match NameE-valueIdentityDescription
Q9SD531.4e-2125.64UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8975.6e-1025.00Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BQ212.8e-238100.00UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004765 PE... [more]
A0A6J1BR428.1e-13766.43UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005004 PE... [more]
A0A5A7VCL17.2e-9348.17UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00248... [more]
A0A1S3BD001.2e-9248.17UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488308 PE=4 SV=1[more]
A0A0A0LPK82.2e-8947.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G381700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31980.18.3e-4130.98unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50160.14.2e-2928.74Plant protein of unknown function (DUF247) [more]
AT3G50120.11.6e-2828.15Plant protein of unknown function (DUF247) [more]
AT3G50150.14.7e-2828.97Plant protein of unknown function (DUF247) [more]
AT5G22550.28.9e-2725.16Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 6..394
e-value: 1.3E-78
score: 265.0
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 8..405
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 8..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g04390.1Moc08g04390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane