Moc08g04320 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc08g04320 vs. NCBI nr
Match: XP_022132066.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 901.7 bits (2329), Expect = 2.4e-258
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MEDDHIETYDLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAY 60
           MEDDHIETYDLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAY
Sbjct: 1   MEDDHIETYDLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAY 60

Query: 61  TPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEH 120
           TPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEH
Sbjct: 61  TPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEH 120

Query: 121 IDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQL 180
           IDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQL
Sbjct: 121 IDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQL 180

Query: 181 PFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFLSFYYALPT 240
           PFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFLSFYYALPT
Sbjct: 181 PFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFLSFYYALPT 240

Query: 241 VTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAF 300
           VTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAF
Sbjct: 241 VTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAF 300

Query: 301 ETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVS 360
           ETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVS
Sbjct: 301 ETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVS 360

Query: 361 KLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATF 420
           KLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATF
Sbjct: 361 KLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATF 420

Query: 421 LVLLTSMQAIYSAISYHKSKP 442
           LVLLTSMQAIYSAISYHKSKP
Sbjct: 421 LVLLTSMQAIYSAISYHKSKP 441

BLAST of Moc08g04320 vs. NCBI nr
Match: XP_022131631.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 558.5 bits (1438), Expect = 5.0e-155
Identity = 280/401 (69.83%), Postives = 323/401 (80.55%), Query Frame = 0

Query: 39  ISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMG 98
           ISEECSIYR+SK LHNIND AYTPQAISIGPFHHG++E MAME+LKL FLD YL +VGM 
Sbjct: 32  ISEECSIYRISKLLHNINDKAYTPQAISIGPFHHGRQELMAMERLKLTFLDCYLSQVGME 91

Query: 99  IEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLN 158
            + AFEIA+GWE RAR+CYAE I+M S +FVK++LVD AFLV  + +             
Sbjct: 92  FDAAFEIARGWEARARQCYAEPINMNSADFVKLLLVDAAFLVMIVDV---------VESG 151

Query: 159 YTLFQAIHVDIYRDLILLENQLPFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISD 218
             LF+AI +DIYRDLILLENQLPFFIL  L +KC++S PFV+F   FCRWY GARELI D
Sbjct: 152 TALFKAIRIDIYRDLILLENQLPFFILVSLFEKCTNSIPFVVFARNFCRWYMGARELIPD 211

Query: 219 KLLTKKPNHLVDFLSFYYALPTVTGKND--KLKYNKRESPPTATELWEAGVEFQKATEDK 278
            LL +KPNHLVDFLSFYYALP  T  ND  K +  K  +PPTATELWEAGV FQKA EDK
Sbjct: 212 GLLARKPNHLVDFLSFYYALPIATENNDHHKNENKKLVNPPTATELWEAGVRFQKAAEDK 271

Query: 279 RLIMDIRF--KDGVLSIPHLEIHDAFETYVRNLLAYEHYHIGDDERCLIQYVYFLDELIS 338
            LIMDI F  ++GVL+IPHLEIHDAFETY+RNLLAYEHYH+GDDERC+IQY+ FLD+LIS
Sbjct: 272 -LIMDIHFQEEEGVLAIPHLEIHDAFETYMRNLLAYEHYHVGDDERCIIQYILFLDDLIS 331

Query: 339 TERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCETWW 398
           TERDVSLLVKAGIITN IGGN+E+VSK+FN+LCKD+++ CDFYYY  ISM L KYCET W
Sbjct: 332 TERDVSLLVKAGIITNGIGGNDEEVSKMFNNLCKDVSVYCDFYYYNQISMTLRKYCETPW 391

Query: 399 HRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAIS 436
           HR  ASL+RDYFN+PW  ISFLAAT  +LLT +Q +YS IS
Sbjct: 392 HRWRASLKRDYFNSPWTSISFLAATVAILLTVVQTVYSVIS 422

BLAST of Moc08g04320 vs. NCBI nr
Match: XP_022155136.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 491.5 bits (1264), Expect = 7.6e-135
Identity = 271/453 (59.82%), Postives = 332/453 (73.29%), Query Frame = 0

Query: 1   MEDDHIETYDLNK-----------KIDEVELEQPHVT---ISMKNMLEKLHPISEECSIY 60
           MED+H+ T+  NK           K++  +L   H +    S+K ML  LHP++EECSIY
Sbjct: 1   MEDNHVGTHQQNKDDGCYGIANIYKVEVGQLSTQHNSNHVSSIKKMLSDLHPMTEECSIY 60

Query: 61  RVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIA 120
           RVSKRLHNI+DMA+ PQ ISIGPFHHG+KE MAMEQLKLRFL  YLRRVGM ++ AF+IA
Sbjct: 61  RVSKRLHNIHDMAFAPQDISIGPFHHGRKELMAMEQLKLRFLHGYLRRVGMEVKAAFDIA 120

Query: 121 QGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIH 180
           + WE RARKCYAE IDMKS++FV MMLVDG FLVE   M Y+++  TQ  L+Y LF AI 
Sbjct: 121 REWEIRARKCYAEPIDMKSEDFVTMMLVDGCFLVELFIMDYEFSG-TQTQLSYPLFSAIS 180

Query: 181 VDIYRDLILLENQLPFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPN 240
            D+  DLI LENQLP+FILECL  K S  +           WY GA  L   K+ T++ N
Sbjct: 181 TDLLHDLIPLENQLPYFILECLF-KISDYSCIDCIVGYLGGWYIGAHVL--PKVPTREAN 240

Query: 241 HLVDFLSFYYALPTVT--GKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRF 300
           HLVD LSFYYA+PTVT  G N +    + E PPTAT+L EAGV+FQKAT+ K  I DI F
Sbjct: 241 HLVDLLSFYYAIPTVTISGNNGQ----EWERPPTATQLKEAGVKFQKATDCKH-ITDISF 300

Query: 301 KDGVLSIPHLEIHDAFETYVRNLLAYEH-YHIGDDERCLIQYVYFLDELISTERDVSLLV 360
           +DGVL IP  +I   FET+VRNLLA+EH YH+G  +RCL QY  FLD+LI+TE+DV+LLV
Sbjct: 301 QDGVLKIPCFQITTTFETHVRNLLAFEHYYHLGHHKRCL-QYFSFLDDLINTEKDVNLLV 360

Query: 361 KAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRR 420
           +AGII+NNIGGNNE+++KLFND+ K  NI+   +YY+ IS++L KYC+T  HR MASLRR
Sbjct: 361 EAGIISNNIGGNNENIAKLFNDMVKHANITSYIFYYSQISLNLRKYCKTQCHRWMASLRR 420

Query: 421 DYFNTPWAFISFLAATFLVLLTSMQAIYSAISY 437
           DYFNTPW FISFLAATFL+LLT +Q +YSA+SY
Sbjct: 421 DYFNTPWTFISFLAATFLILLTVVQTLYSALSY 443

BLAST of Moc08g04320 vs. NCBI nr
Match: XP_022131632.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 446.8 bits (1148), Expect = 2.1e-121
Identity = 251/454 (55.29%), Postives = 308/454 (67.84%), Query Frame = 0

Query: 1   MEDDHIETY---------------DLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSI 60
           MED+HIET+               D N +++E +    HV +S++N L+KLHPI+EECSI
Sbjct: 1   MEDNHIETHQQKNNDDACYGISNMDENNEVEETQGNSIHV-VSIENRLKKLHPITEECSI 60

Query: 61  YRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEI 120
           YRVSKRLHNI+D A+ PQAISIGPFHHG++E MAMEQLKLRFL  YLRRVGM ++ AF+I
Sbjct: 61  YRVSKRLHNIHDTAFAPQAISIGPFHHGREELMAMEQLKLRFLHGYLRRVGMDVKAAFKI 120

Query: 121 AQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAI 180
           A+ WETRARKCYAE IDMKS++FV MMLVDG FLVEF  M Y+++               
Sbjct: 121 ARDWETRARKCYAEPIDMKSEDFVTMMLVDGCFLVEFFIMGYEFS--------------- 180

Query: 181 HVDIYRDLILLENQLPFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKP 240
                                       S T ++        WY G   L   K+  ++ 
Sbjct: 181 ---------------------------GSQTQYL------GGWYIGVARL--PKVPAREA 240

Query: 241 NHLVDFLSFYYALPTVT--GKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIR 300
           NHLVD LSFYYA+PTVT  G N+  K+   E PPTAT+L EAGV FQKA + K  I DI 
Sbjct: 241 NHLVDLLSFYYAIPTVTISGNNNGQKW---ERPPTATQLKEAGVRFQKAIDWKH-ITDIS 300

Query: 301 FKDGVLSIPHLEIHDAFETYVRNLLAYEH-YHIGDDERCLIQYVYFLDELISTERDVSLL 360
           F+DGVL IP  +I  +FET VRNLLA+EH YH+G D++CL QY  FLD+LISTE+DV LL
Sbjct: 301 FRDGVLKIPCFQITTSFETRVRNLLAFEHYYHLGRDKKCL-QYFSFLDDLISTEKDVGLL 360

Query: 361 VKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLR 420
           VKAGII+NNIGGN+ED++KLFND+ K  NIS  FYYY+ IS+DL KYC+T WHR  ASL+
Sbjct: 361 VKAGIISNNIGGNHEDIAKLFNDMVKYGNISSSFYYYSQISLDLRKYCDTPWHRWKASLK 398

Query: 421 RDYFNTPWAFISFLAATFLVLLTSMQAIYSAISY 437
           RDYFN+PW  ISFLAATFL+LLT +Q +YSA+ Y
Sbjct: 421 RDYFNSPWTSISFLAATFLILLTVVQTLYSALQY 398

BLAST of Moc08g04320 vs. NCBI nr
Match: XP_022158990.1 (UPF0481 protein At3g47200-like isoform X2 [Momordica charantia])

HSP 1 Score: 407.5 bits (1046), Expect = 1.4e-109
Identity = 232/429 (54.08%), Postives = 287/429 (66.90%), Query Frame = 0

Query: 25  VTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLK 84
           V  S+K ML++L P++EEC+I+RV +RL   N  AY PQ ISIGPFHHG+++ M MEQ K
Sbjct: 31  VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 90

Query: 85  LRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIR 144
           LRFLD YLRR   GIE    I + WET AR CYAE I+M SD FVKMMLVDG F+VE + 
Sbjct: 91  LRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELMM 150

Query: 145 MHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQLPFFILECLLDKCS--SSTPFVLFT 204
           M  +  + T+   +  LF A+  D+Y DLI+LENQLPFF+L+ L D+ S  +   F+  T
Sbjct: 151 MVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQLT 210

Query: 205 STFCRWYTGARELISDKLL---------TKKPNHLVDFLSFYY----ALPTVTGKNDKLK 264
             F   YT    LI  + L         T K NHLVDFLSFYY    A  + T  +  + 
Sbjct: 211 HMF---YTRG-PLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIS 270

Query: 265 YNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLA 324
             K   PPT TELWEAG+ F+KA   K  IMDI FKD VL IP LEI D FETYVRNL+A
Sbjct: 271 RKKCTFPPTVTELWEAGIVFKKAMRAKH-IMDISFKDRVLQIPPLEIGDVFETYVRNLMA 330

Query: 325 YEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKD 384
           +E YH  +D +  IQY  FL+ LIS E+DVSLLVKA IITN IGGNN++VS LFNDLCKD
Sbjct: 331 FEQYH--NDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKD 390

Query: 385 INISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQA 439
           + +  D   +  I+  LH++C   W++ MASLRRDYFNTPWAFISF+AA FL+LLT +Q 
Sbjct: 391 VIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQT 450

BLAST of Moc08g04320 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.9e-35
Identity = 116/426 (27.23%), Postives = 197/426 (46.24%), Query Frame = 0

Query: 41  EECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYL---RRVGM 100
           E C I+RV +    +N  AY P+ +SIGP+H+G+K    ++Q K R L  +L   ++  +
Sbjct: 44  ESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDV 103

Query: 101 GIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAF-LVEFIRMHYQWATMTQPN 160
                 +     E + RK Y+E +    D  + MM++DG F L+ F+ M         P 
Sbjct: 104 EENVLVKAVVDLEDKIRKSYSEELKTGHD-LMFMMVLDGCFILMVFLIMSGNIELSEDPI 163

Query: 161 LNYTLFQAIHVDIYRDLILLENQLPFFILECLL--DKCSSSTPFVLFTSTFCRWYTGARE 220
            +     +    I  DL+LLENQ+PFF+L+ L    K   S+        F +       
Sbjct: 164 FSIPWLLS---SIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFHFFKNPIDKEG 223

Query: 221 LISDKLLTKKPNHLVDFLSFYYALPTVTGKNDK---------LKYNKRESPP-------- 280
              +K    K  HL+D +   + LP  T ++DK         L   K  + P        
Sbjct: 224 SYWEKHRNYKAKHLLDLIRETF-LPN-TSESDKASSPHVQVQLHEGKSGNVPSVDSKAVP 283

Query: 281 ---TATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLAYEHYH 340
              +A  L   G++F+     +  I+++R K   L IP L       ++  N +A+E ++
Sbjct: 284 LILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFEQFY 343

Query: 341 IGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISC 400
             D    +  Y+ F+  L++ E DV+ L    +I  N  G+N +VS+ F  + KD+    
Sbjct: 344 T-DSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISKDVVFEV 403

Query: 401 DFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAI 441
           D  Y  ++   +++Y + W++   A  R  +F +PW F+S  A  F++LLT +Q+  + +
Sbjct: 404 DTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVILLTMLQSTVAIL 462

BLAST of Moc08g04320 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.1e-19
Identity = 108/481 (22.45%), Postives = 195/481 (40.54%), Query Frame = 0

Query: 44  SIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRV-GMGIEDA 103
           SI+ V K L   +  +YTP  +SIGP+H  + E   ME+ KL        +       D 
Sbjct: 44  SIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHDL 103

Query: 104 FEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLF 163
            E  Q  E + R CY ++I    +  + +M VD +FL+EF++++      T  N      
Sbjct: 104 VEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFLIEFLKIYSFRKVETLIN------ 163

Query: 164 QAIHVDIYRDLILLENQLPFFI----LECLLDKCSSSTPFVLFTST-FCRWYTGARELIS 223
           +  H +I RD++++ENQ+P F+    LE  L+   S+   +L   T  C+  +       
Sbjct: 164 RVGHNEILRDIMMIENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFD 223

Query: 224 DKLLTK----KPNHLVDFLSFYYALPTV----------------TGKN------DKLKYN 283
           D  + K    + NH++DFL +   +P +                 G N      D++K+ 
Sbjct: 224 DDQILKAQFQECNHILDFL-YQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKHQ 283

Query: 284 -KR-------------------------------------------------------ES 343
            KR                                                       E 
Sbjct: 284 FKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIEK 343

Query: 344 P--------PTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNL 403
           P        P+ ++L +AGV F+         +      G   +P + +    ET +RNL
Sbjct: 344 PPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNL 403

Query: 404 LAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLC 429
           +AYE  +         +Y   ++ +I +E DV LL + G++ + +  ++++ ++++N + 
Sbjct: 404 VAYEATNT-SGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRL-KSDQEAAEMWNGMS 463

BLAST of Moc08g04320 vs. ExPASy TrEMBL
Match: A0A6J1BR71 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005028 PE=4 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.2e-258
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MEDDHIETYDLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAY 60
           MEDDHIETYDLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAY
Sbjct: 1   MEDDHIETYDLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAY 60

Query: 61  TPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEH 120
           TPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEH
Sbjct: 61  TPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEH 120

Query: 121 IDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQL 180
           IDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQL
Sbjct: 121 IDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQL 180

Query: 181 PFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFLSFYYALPT 240
           PFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFLSFYYALPT
Sbjct: 181 PFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFLSFYYALPT 240

Query: 241 VTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAF 300
           VTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAF
Sbjct: 241 VTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAF 300

Query: 301 ETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVS 360
           ETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVS
Sbjct: 301 ETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVS 360

Query: 361 KLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATF 420
           KLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATF
Sbjct: 361 KLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATF 420

Query: 421 LVLLTSMQAIYSAISYHKSKP 442
           LVLLTSMQAIYSAISYHKSKP
Sbjct: 421 LVLLTSMQAIYSAISYHKSKP 441

BLAST of Moc08g04320 vs. ExPASy TrEMBL
Match: A0A6J1BQ17 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004760 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.4e-155
Identity = 280/401 (69.83%), Postives = 323/401 (80.55%), Query Frame = 0

Query: 39  ISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMG 98
           ISEECSIYR+SK LHNIND AYTPQAISIGPFHHG++E MAME+LKL FLD YL +VGM 
Sbjct: 32  ISEECSIYRISKLLHNINDKAYTPQAISIGPFHHGRQELMAMERLKLTFLDCYLSQVGME 91

Query: 99  IEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLN 158
            + AFEIA+GWE RAR+CYAE I+M S +FVK++LVD AFLV  + +             
Sbjct: 92  FDAAFEIARGWEARARQCYAEPINMNSADFVKLLLVDAAFLVMIVDV---------VESG 151

Query: 159 YTLFQAIHVDIYRDLILLENQLPFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISD 218
             LF+AI +DIYRDLILLENQLPFFIL  L +KC++S PFV+F   FCRWY GARELI D
Sbjct: 152 TALFKAIRIDIYRDLILLENQLPFFILVSLFEKCTNSIPFVVFARNFCRWYMGARELIPD 211

Query: 219 KLLTKKPNHLVDFLSFYYALPTVTGKND--KLKYNKRESPPTATELWEAGVEFQKATEDK 278
            LL +KPNHLVDFLSFYYALP  T  ND  K +  K  +PPTATELWEAGV FQKA EDK
Sbjct: 212 GLLARKPNHLVDFLSFYYALPIATENNDHHKNENKKLVNPPTATELWEAGVRFQKAAEDK 271

Query: 279 RLIMDIRF--KDGVLSIPHLEIHDAFETYVRNLLAYEHYHIGDDERCLIQYVYFLDELIS 338
            LIMDI F  ++GVL+IPHLEIHDAFETY+RNLLAYEHYH+GDDERC+IQY+ FLD+LIS
Sbjct: 272 -LIMDIHFQEEEGVLAIPHLEIHDAFETYMRNLLAYEHYHVGDDERCIIQYILFLDDLIS 331

Query: 339 TERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCETWW 398
           TERDVSLLVKAGIITN IGGN+E+VSK+FN+LCKD+++ CDFYYY  ISM L KYCET W
Sbjct: 332 TERDVSLLVKAGIITNGIGGNDEEVSKMFNNLCKDVSVYCDFYYYNQISMTLRKYCETPW 391

Query: 399 HRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAIS 436
           HR  ASL+RDYFN+PW  ISFLAAT  +LLT +Q +YS IS
Sbjct: 392 HRWRASLKRDYFNSPWTSISFLAATVAILLTVVQTVYSVIS 422

BLAST of Moc08g04320 vs. ExPASy TrEMBL
Match: A0A6J1DQT2 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111022276 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 3.7e-135
Identity = 271/453 (59.82%), Postives = 332/453 (73.29%), Query Frame = 0

Query: 1   MEDDHIETYDLNK-----------KIDEVELEQPHVT---ISMKNMLEKLHPISEECSIY 60
           MED+H+ T+  NK           K++  +L   H +    S+K ML  LHP++EECSIY
Sbjct: 1   MEDNHVGTHQQNKDDGCYGIANIYKVEVGQLSTQHNSNHVSSIKKMLSDLHPMTEECSIY 60

Query: 61  RVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIA 120
           RVSKRLHNI+DMA+ PQ ISIGPFHHG+KE MAMEQLKLRFL  YLRRVGM ++ AF+IA
Sbjct: 61  RVSKRLHNIHDMAFAPQDISIGPFHHGRKELMAMEQLKLRFLHGYLRRVGMEVKAAFDIA 120

Query: 121 QGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAIH 180
           + WE RARKCYAE IDMKS++FV MMLVDG FLVE   M Y+++  TQ  L+Y LF AI 
Sbjct: 121 REWEIRARKCYAEPIDMKSEDFVTMMLVDGCFLVELFIMDYEFSG-TQTQLSYPLFSAIS 180

Query: 181 VDIYRDLILLENQLPFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPN 240
            D+  DLI LENQLP+FILECL  K S  +           WY GA  L   K+ T++ N
Sbjct: 181 TDLLHDLIPLENQLPYFILECLF-KISDYSCIDCIVGYLGGWYIGAHVL--PKVPTREAN 240

Query: 241 HLVDFLSFYYALPTVT--GKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRF 300
           HLVD LSFYYA+PTVT  G N +    + E PPTAT+L EAGV+FQKAT+ K  I DI F
Sbjct: 241 HLVDLLSFYYAIPTVTISGNNGQ----EWERPPTATQLKEAGVKFQKATDCKH-ITDISF 300

Query: 301 KDGVLSIPHLEIHDAFETYVRNLLAYEH-YHIGDDERCLIQYVYFLDELISTERDVSLLV 360
           +DGVL IP  +I   FET+VRNLLA+EH YH+G  +RCL QY  FLD+LI+TE+DV+LLV
Sbjct: 301 QDGVLKIPCFQITTTFETHVRNLLAFEHYYHLGHHKRCL-QYFSFLDDLINTEKDVNLLV 360

Query: 361 KAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRR 420
           +AGII+NNIGGNNE+++KLFND+ K  NI+   +YY+ IS++L KYC+T  HR MASLRR
Sbjct: 361 EAGIISNNIGGNNENIAKLFNDMVKHANITSYIFYYSQISLNLRKYCKTQCHRWMASLRR 420

Query: 421 DYFNTPWAFISFLAATFLVLLTSMQAIYSAISY 437
           DYFNTPW FISFLAATFL+LLT +Q +YSA+SY
Sbjct: 421 DYFNTPWTFISFLAATFLILLTVVQTLYSALSY 443

BLAST of Moc08g04320 vs. ExPASy TrEMBL
Match: A0A6J1BRK2 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004762 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.0e-121
Identity = 251/454 (55.29%), Postives = 308/454 (67.84%), Query Frame = 0

Query: 1   MEDDHIETY---------------DLNKKIDEVELEQPHVTISMKNMLEKLHPISEECSI 60
           MED+HIET+               D N +++E +    HV +S++N L+KLHPI+EECSI
Sbjct: 1   MEDNHIETHQQKNNDDACYGISNMDENNEVEETQGNSIHV-VSIENRLKKLHPITEECSI 60

Query: 61  YRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEI 120
           YRVSKRLHNI+D A+ PQAISIGPFHHG++E MAMEQLKLRFL  YLRRVGM ++ AF+I
Sbjct: 61  YRVSKRLHNIHDTAFAPQAISIGPFHHGREELMAMEQLKLRFLHGYLRRVGMDVKAAFKI 120

Query: 121 AQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLFQAI 180
           A+ WETRARKCYAE IDMKS++FV MMLVDG FLVEF  M Y+++               
Sbjct: 121 ARDWETRARKCYAEPIDMKSEDFVTMMLVDGCFLVEFFIMGYEFS--------------- 180

Query: 181 HVDIYRDLILLENQLPFFILECLLDKCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKP 240
                                       S T ++        WY G   L   K+  ++ 
Sbjct: 181 ---------------------------GSQTQYL------GGWYIGVARL--PKVPAREA 240

Query: 241 NHLVDFLSFYYALPTVT--GKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIR 300
           NHLVD LSFYYA+PTVT  G N+  K+   E PPTAT+L EAGV FQKA + K  I DI 
Sbjct: 241 NHLVDLLSFYYAIPTVTISGNNNGQKW---ERPPTATQLKEAGVRFQKAIDWKH-ITDIS 300

Query: 301 FKDGVLSIPHLEIHDAFETYVRNLLAYEH-YHIGDDERCLIQYVYFLDELISTERDVSLL 360
           F+DGVL IP  +I  +FET VRNLLA+EH YH+G D++CL QY  FLD+LISTE+DV LL
Sbjct: 301 FRDGVLKIPCFQITTSFETRVRNLLAFEHYYHLGRDKKCL-QYFSFLDDLISTEKDVGLL 360

Query: 361 VKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLR 420
           VKAGII+NNIGGN+ED++KLFND+ K  NIS  FYYY+ IS+DL KYC+T WHR  ASL+
Sbjct: 361 VKAGIISNNIGGNHEDIAKLFNDMVKYGNISSSFYYYSQISLDLRKYCDTPWHRWKASLK 398

Query: 421 RDYFNTPWAFISFLAATFLVLLTSMQAIYSAISY 437
           RDYFN+PW  ISFLAATFL+LLT +Q +YSA+ Y
Sbjct: 421 RDYFNSPWTSISFLAATFLILLTVVQTLYSALQY 398

BLAST of Moc08g04320 vs. ExPASy TrEMBL
Match: A0A6J1DXD6 (UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 7.0e-110
Identity = 232/429 (54.08%), Postives = 287/429 (66.90%), Query Frame = 0

Query: 25  VTISMKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLK 84
           V  S+K ML++L P++EEC+I+RV +RL   N  AY PQ ISIGPFHHG+++ M MEQ K
Sbjct: 31  VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 90

Query: 85  LRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIR 144
           LRFLD YLRR   GIE    I + WET AR CYAE I+M SD FVKMMLVDG F+VE + 
Sbjct: 91  LRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELMM 150

Query: 145 MHYQWATMTQPNLNYTLFQAIHVDIYRDLILLENQLPFFILECLLDKCS--SSTPFVLFT 204
           M  +  + T+   +  LF A+  D+Y DLI+LENQLPFF+L+ L D+ S  +   F+  T
Sbjct: 151 MVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAGLSFLQLT 210

Query: 205 STFCRWYTGARELISDKLL---------TKKPNHLVDFLSFYY----ALPTVTGKNDKLK 264
             F   YT    LI  + L         T K NHLVDFLSFYY    A  + T  +  + 
Sbjct: 211 HMF---YTRG-PLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLAIS 270

Query: 265 YNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLA 324
             K   PPT TELWEAG+ F+KA   K  IMDI FKD VL IP LEI D FETYVRNL+A
Sbjct: 271 RKKCTFPPTVTELWEAGIVFKKAMRAKH-IMDISFKDRVLQIPPLEIGDVFETYVRNLMA 330

Query: 325 YEHYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKD 384
           +E YH  +D +  IQY  FL+ LIS E+DVSLLVKA IITN IGGNN++VS LFNDLCKD
Sbjct: 331 FEQYH--NDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDLCKD 390

Query: 385 INISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQA 439
           + +  D   +  I+  LH++C   W++ MASLRRDYFNTPWAFISF+AA FL+LLT +Q 
Sbjct: 391 VIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTFLQT 450

BLAST of Moc08g04320 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 252.3 bits (643), Expect = 7.2e-67
Identity = 160/420 (38.10%), Postives = 237/420 (56.43%), Query Frame = 0

Query: 28  SMKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRF 87
           S+K  L  L  +S +C IY+V  +L  +N  AYTP+ +S GP H G++E  AME  K R+
Sbjct: 278 SIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQKYRY 337

Query: 88  LDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVE-FIRMH 147
           L +++ R    +ED   +A+ WE  AR CYAE + + SD FV+M++VDG+FLVE  +R H
Sbjct: 338 LLSFIPRTNSSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLVELLLRSH 397

Query: 148 YQWATMTQPNL---NYTLF--QAIHVDIYRDLILLENQLPFFILE----CLLDKCSSSTP 207
           Y       P L   N  +F    +  D+ RD+IL+ENQLPFF+++     LL+     TP
Sbjct: 398 Y-------PRLRGENDRIFGNSMMITDVCRDMILIENQLPFFVVKEIFLLLLNYYQQGTP 457

Query: 208 FVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFLSFYYALPTVTGKNDKLKYN--KRES 267
            ++  +   R ++     I D+    +P H VD L   Y LP       KL+Y   K ++
Sbjct: 458 SIIQLAQ--RHFSYFLSRIDDEKFITEPEHFVDLLRSCY-LPQFP---IKLEYTTVKVDN 517

Query: 268 PPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLAYEHYHI 327
            P ATEL  AGV F K  E    ++DI F DGVL IP + + D  E+  +N++ +E    
Sbjct: 518 APEATELHTAGVRF-KPAETSSCLLDISFADGVLKIPTIVVDDLTESLYKNIIGFEQCRC 577

Query: 328 GDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCD 387
            +  +  + Y+  L   I +  D  LL+ +GII N + GN+ DVS LFN + K++ I   
Sbjct: 578 SN--KNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYL-GNSVDVSNLFNSISKEV-IYDR 637

Query: 388 FYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAIS 436
            +Y++ +S +L  YC T W+R  A LRRDYF+ PWA  S  AA  L+LLT +Q++ S ++
Sbjct: 638 RFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLTFIQSVCSILA 679

BLAST of Moc08g04320 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 203.4 bits (516), Expect = 3.8e-52
Identity = 140/454 (30.84%), Postives = 234/454 (51.54%), Query Frame = 0

Query: 27  ISMKNMLEKLHPISE-----ECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAME 86
           IS+ + LE+ H   +     +  IYRV   L   ++ +Y PQ +S+GP+HHG+K   +M+
Sbjct: 82  ISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRSMD 141

Query: 87  QLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVE 146
           + K R ++  L+R   GI+   +  +  E +AR CY   + + S+ F++M+++DG F++E
Sbjct: 142 RHKWRAVNRVLKRTNQGIKMYIDAMRELEEKARACYEGPLSLSSNEFIEMLVLDGCFVLE 201

Query: 147 FIRMHYQWATMTQPNLNYTLFQ---AIHVDIYRDLILLENQLPFFILECLLD-KCSSSTP 206
             R   +  T      N  +F    ++H  I RD+++LENQLP F+L  LL+ +  +   
Sbjct: 202 LFRGAVEGFTELGYARNDPVFAMRGSMH-SIQRDMVMLENQLPLFVLNRLLELQLGTRNQ 261

Query: 207 FVLFTSTFCRWY--------------------TGARELISDKLLTKKPNHLVDFLSFYYA 266
             L      R++                    + AR+   D        H +D   F  +
Sbjct: 262 TGLVAQLAIRFFDPLMPTDEPLTKSGQSKLENSLARDKSFDPFADMGELHCLDV--FRRS 321

Query: 267 LPTVTGKNDKLKYNKRESPPT-------------ATELWEAGVEFQKATEDKRLIMDIRF 326
           L   + K +     KR S  T              TEL EAG++F++   D+    D++F
Sbjct: 322 LLRSSPKPEPRLTRKRWSRNTRVADKRRQQLIHCVTELKEAGIKFRRRKTDR--FWDMQF 381

Query: 327 KDGVLSIPHLEIHDAFETYVRNLLAYEHYHIGDDERCLIQYVYFLDELISTERDVSLLVK 386
           K+G L IP L IHD  ++   NL+A+E  HI D    +  Y+ F+D LI +  DVS L  
Sbjct: 382 KNGYLEIPRLLIHDGTKSLFLNLIAFEQCHI-DSSNDITSYIIFMDNLIDSHEDVSYLHY 441

Query: 387 AGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCETWWHRSMASLRRD 439
            GII + +G ++E V+ LFN LC+++    +  Y + +S+++++Y +  W+   A+L+  
Sbjct: 442 CGIIEHWLGSDSE-VADLFNRLCQEVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHK 501

BLAST of Moc08g04320 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 196.4 bits (498), Expect = 4.7e-50
Identity = 148/470 (31.49%), Postives = 228/470 (48.51%), Query Frame = 0

Query: 17  EVELEQPHVT------ISMKNMLEKLH-----PISEECSIYRVSKRLHNINDMAYTPQAI 76
           EV  E+P  T      IS+++ LE+        I  +  IYRV   L   +  +Y PQ +
Sbjct: 76  EVVEERPEETTGDSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKKSYFPQTV 135

Query: 77  SIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKS 136
           S+GP+HHG+K    ME+ K R L+  L+R+   IE      +  E +AR CY   I +  
Sbjct: 136 SLGPYHHGKKRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKARACYEGPISLSR 195

Query: 137 DNFVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLF--QAIHVDIYRDLILLENQLPFF 196
           + F +M+++DG F++E  R   +  T      N  +F  + +   I RD+I+LENQLP F
Sbjct: 196 NEFTEMLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAMRGLMHSIQRDMIMLENQLPLF 255

Query: 197 ILECLLD---KCSSSTPFVLFT--------------------STFCRWYTGARELISDK- 256
           +L+ LL+      + T  V                       S    W   + + + DK 
Sbjct: 256 VLDRLLELQLGTQNQTGIVAHVAVKFFDPLMPTGEALTKPDQSKLMNWLEKSLDTLGDKG 315

Query: 257 -----------LLTKKPNHLVDFLSFYYALPTVTGKNDKLKYNKRESPPTATELWEAGVE 316
                      LL   P      L     L  +T     +   +++     TEL EAGV+
Sbjct: 316 ELHCLDVFRRSLLQSSPTPNTRSL-----LKRLTRNTRVVDKRQQQLVHCVTELREAGVK 375

Query: 317 FQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLAYEHYHIGDDERCLIQYVYF 376
           F+K   D+    DI FK+G L IP L IHD  ++   NL+A+E  HI +    +  Y+ F
Sbjct: 376 FRKRKTDR--FWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHI-ESSNHITSYIIF 435

Query: 377 LDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHK 436
           +D LI++  DVS L   GII + +G ++E V+ LFN LC+++       + + +S D+++
Sbjct: 436 MDNLINSSEDVSYLHYCGIIEHWLGSDSE-VADLFNRLCQEVVFDPKDSHLSRLSGDVNR 495

Query: 437 YCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAISYHK 439
           Y    W+   A+L   YFN PWA+ SF AA  L+LLT  Q+ Y+  +Y+K
Sbjct: 496 YYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYAVYAYYK 536

BLAST of Moc08g04320 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 196.1 bits (497), Expect = 6.1e-50
Identity = 153/471 (32.48%), Postives = 235/471 (49.89%), Query Frame = 0

Query: 4   DHIETYDLNKKIDEVELEQPHVT-----ISMKNMLEKLHPIS-----EECSIYRVSKRLH 63
           +H+ETY    KI EV+ E+P  T     IS+K+ +EK          ++  IYRV   L 
Sbjct: 42  NHVETYVEPSKI-EVKEEKPRETREEWVISIKDKMEKALSYDATNSWDKLCIYRVPFYLQ 101

Query: 64  NINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRA 123
             +  +Y PQ +SIGP+HHG+     ME+ K R ++  + R    IE   +  +  E  A
Sbjct: 102 ENDKKSYLPQTVSIGPYHHGKVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMKELEEEA 161

Query: 124 RKCYAEHIDMKSDN-FVKMMLVDGAFLVEFIRMHYQWATMTQPNLNYTLF--QAIHVDIY 183
           R CY   IDMK+ N F +M+++DG F++E  +   Q         N  +F  + +   I 
Sbjct: 162 RACYQGPIDMKNSNEFTEMLVLDGCFVLELFKGTIQGFQKIGYARNDPVFAKRGLMHSIQ 221

Query: 184 RDLILLENQLPFFILECLLDKCSSSTP-----FVLFTSTFCRWYTGARELI--------- 243
           RD+I+LENQLP F+L+ LL    + TP            F +      E++         
Sbjct: 222 RDMIMLENQLPLFVLDRLLG-LQTGTPNQTGIVAEVAVRFFKTLMPTSEVLTKSERSLDS 281

Query: 244 ---SDKLLTKKPNHLVDFL--SFYYALPTV---TGKNDKLKYNKRES-PPTATELWEAGV 303
              SD+L      H +D    S   +  T    T   D     K++      TEL  AGV
Sbjct: 282 QEKSDELGDNGGLHCLDVFHRSLIQSSETTNQGTPYEDMSMVEKQQQLIHCVTELRGAGV 341

Query: 304 EFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLAYEHYHIGDDERCLIQYVY 363
            F +  ++   + DI FK+G L IP L IHD  ++   NL+A+E  H       +  Y+ 
Sbjct: 342 NFMR--KETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNN-ITSYII 401

Query: 364 FLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLH 423
           F+D LI++ +DVS L   GII + +G ++E V+ LFN LCK++       Y + +S +++
Sbjct: 402 FMDNLINSSQDVSYLHHDGIIEHWLGSDSE-VADLFNRLCKEVIFDPKDGYLSQLSREVN 461

Query: 424 KYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAISYHK 439
           +Y    W+   A+LR+ YFN PWA+ SF AA  L+ LT  Q+ ++  +Y+K
Sbjct: 462 RYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVYAYYK 506

BLAST of Moc08g04320 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 195.7 bits (496), Expect = 8.0e-50
Identity = 134/418 (32.06%), Postives = 215/418 (51.44%), Query Frame = 0

Query: 29  MKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRFL 88
           MK + +      +   IYRV   L   +  +Y PQ +SIGP+HHG K  M ME+ K R +
Sbjct: 90  MKTLGDNATTSWDNLCIYRVPPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAV 149

Query: 89  DAYLRRVGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMHYQ 148
           +  + R    IE   +  +  E +AR CY   I+M  + F++M+++DG F++E  +   +
Sbjct: 150 NMVMARAKHDIEMYIDAMKELEEKARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSE 209

Query: 149 WATMT--QPNLNYTLFQAIHVDIYRDLILLENQLPFFILECLLDKCSSSTPFVLFTSTFC 208
                   PN      + +   I RD+++LENQLP+ +L+ LL          +    F 
Sbjct: 210 GFQEIGYAPNDPVFGMRGLMQSIRRDMVMLENQLPWSVLKGLLQLQRPDVLDKVNVQLFQ 269

Query: 209 RWYTGARELISDKLLTKKPN-HLVDFLSFYYALPTVTGKNDKLKYNKRESP--PTATELW 268
            ++     L + ++LT++   H +D L       + T   D    NK+        TEL 
Sbjct: 270 PFFQPL--LPTREVLTEEGGLHCLDVLRRGLLQSSGTSDEDMSMVNKQPQQLIHCVTELR 329

Query: 269 EAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLAYEHYHIGDDERCLI 328
            AGVEF +  ++     DI FK+G L IP L IHD  ++   NL+A+E  HI   ++ + 
Sbjct: 330 NAGVEFMR--KETGHFWDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFEQCHIKSSKK-IT 389

Query: 329 QYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADIS 388
            Y+ F+D LI++  DVS L   GII N +G ++E VS LFN L K++    +  Y + ++
Sbjct: 390 SYIIFMDNLINSSEDVSYLHHYGIIENWLGSDSE-VSDLFNGLGKEVIFDPNDGYLSALT 449

Query: 389 MDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAISYHKSKP 442
            +++ Y    W+   A+LR  YFN PWA+ SF+AA  L++ T  Q+ ++  +Y K  P
Sbjct: 450 GEVNIYYRRKWNYLKATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFAVFAYFKPPP 501