Moc08g03360 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc08g03360 vs. NCBI nr
Match: XP_022131900.1 (ER membrane protein complex subunit 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1936.8 bits (5016), Expect = 0.0e+00
Identity = 985/985 (100.00%), Postives = 985/985 (100.00%), Query Frame = 0

Query: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
           MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV
Sbjct: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60

Query: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
           VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ
Sbjct: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120

Query: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
           MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV
Sbjct: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180

Query: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
           EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV
Sbjct: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240

Query: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
           LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL
Sbjct: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300

Query: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
           IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE
Sbjct: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360

Query: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
           ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII
Sbjct: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420

Query: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
           NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS
Sbjct: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480

Query: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
           KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP
Sbjct: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540

Query: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
           HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE
Sbjct: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600

Query: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
           QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD
Sbjct: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660

Query: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
           YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA
Sbjct: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720

Query: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
           PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780

Query: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
           HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840

Query: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
           VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT
Sbjct: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900

Query: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
           YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT
Sbjct: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960

Query: 961 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALVVAIFVTWGLSERKELQDKWK
Sbjct: 961 IVALVVAIFVTWGLSERKELQDKWK 985

BLAST of Moc08g03360 vs. NCBI nr
Match: XP_022131901.1 (ER membrane protein complex subunit 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1871.3 bits (4846), Expect = 0.0e+00
Identity = 949/949 (100.00%), Postives = 949/949 (100.00%), Query Frame = 0

Query: 37  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 96
           RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA
Sbjct: 21  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 80

Query: 97  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 156
           LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR
Sbjct: 81  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 140

Query: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 216
           SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE
Sbjct: 141 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 200

Query: 217 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 276
           LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS
Sbjct: 201 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 260

Query: 277 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 336
           GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL
Sbjct: 261 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 320

Query: 337 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 396
           VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME
Sbjct: 321 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 380

Query: 397 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 456
           DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG
Sbjct: 381 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 440

Query: 457 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 516
           TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL
Sbjct: 441 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 500

Query: 517 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 576
           LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK
Sbjct: 501 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 560

Query: 577 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 636
           EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY
Sbjct: 561 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 620

Query: 637 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 696
           SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA
Sbjct: 621 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 680

Query: 697 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 756
           KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH
Sbjct: 681 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 740

Query: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 816
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS
Sbjct: 741 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 800

Query: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 801 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 860

Query: 877 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 936
           NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF
Sbjct: 861 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 920

Query: 937 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 986
           TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK
Sbjct: 921 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 969

BLAST of Moc08g03360 vs. NCBI nr
Match: KAG6585394.1 (ER membrane protein complex subunit 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1783.1 bits (4617), Expect = 0.0e+00
Identity = 884/985 (89.75%), Postives = 940/985 (95.43%), Query Frame = 0

Query: 1    MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
            MVL +KVYLLL + LFAS ANYGFSLYEDQVGLMDWRQQYLGK KHALFHS KSGRKRVV
Sbjct: 35   MVLTVKVYLLLFVTLFASLANYGFSLYEDQVGLMDWRQQYLGKAKHALFHSSKSGRKRVV 94

Query: 61   VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
            VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF LGKY++SLSSEGNFLRAWNLPDGQ
Sbjct: 95   VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFVLGKYVVSLSSEGNFLRAWNLPDGQ 154

Query: 121  MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
            MVWESF+QGT PSKSLLLVPKSLK NQETVILVFG SCLHAVSSLDGEVIWKIDLTENSV
Sbjct: 155  MVWESFLQGTNPSKSLLLVPKSLKANQETVILVFGSSCLHAVSSLDGEVIWKIDLTENSV 214

Query: 181  EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
            EIQ+IIQHH+SD IYAVGFSSPTQFD+FQIN+KSGEL+KH T TF+GGFSGELVSVSDDV
Sbjct: 215  EIQKIIQHHESDTIYAVGFSSPTQFDRFQINVKSGELVKHHTATFSGGFSGELVSVSDDV 274

Query: 241  LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
            LVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE SGS+EIVPSKLSG+ A++V S L L
Sbjct: 275  LVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELSGSIEIVPSKLSGLFAVKVDSRLTL 334

Query: 301  IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
            +RVKGEGELEVVDKIH QATLSD+LLVSE Q A ALVQ +GS++ L+VKLID+WSS+F++
Sbjct: 335  VRVKGEGELEVVDKIHSQATLSDALLVSEGQHAAALVQHEGSHVRLTVKLIDNWSSNFID 394

Query: 361  ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
            ENIVID  RG   KVFL+SYIRTDRSHGFRAL+VMEDHSLLLVQQGE+VWSREDGLASI+
Sbjct: 395  ENIVIDGHRGTFQKVFLHSYIRTDRSHGFRALLVMEDHSLLLVQQGEVVWSREDGLASIV 454

Query: 421  NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
            NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS
Sbjct: 455  NVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 514

Query: 481  KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
            K+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS LLHP  KSEAC PRWLNIYQWQDP
Sbjct: 515  KMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSRLLHPSHKSEACAPRWLNIYQWQDP 574

Query: 541  HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
            HHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+EI SSSQ+HSV+QVIPLP TDSTE
Sbjct: 575  HHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGREISSSSQIHSVLQVIPLPFTDSTE 634

Query: 601  QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
            QRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWYSVEADSGVIKGH+LKRNCI ++DD
Sbjct: 635  QRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWYSVEADSGVIKGHSLKRNCIGVVDD 694

Query: 661  YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
            YCFESKDVWSI+LPSE+EKII+TA R LNEVVHTQAKV ADQDVMYKYISKNLLFLATVA
Sbjct: 695  YCFESKDVWSIILPSETEKIIATAARKLNEVVHTQAKVVADQDVMYKYISKNLLFLATVA 754

Query: 721  PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
            PKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 755  PKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 814

Query: 781  HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
            HRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 815  HRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 874

Query: 841  VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
            VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINP+QAEREEGVIPLTDSLPIIPQT
Sbjct: 875  VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPTQAEREEGVIPLTDSLPIIPQT 934

Query: 901  YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
            YATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLL+T
Sbjct: 935  YATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLIT 994

Query: 961  IVALVVAIFVTWGLSERKELQDKWK 986
            IVALV+AIF TW  SERKELQDKWK
Sbjct: 995  IVALVIAIFATWVFSERKELQDKWK 1019

BLAST of Moc08g03360 vs. NCBI nr
Match: XP_022951478.1 (ER membrane protein complex subunit 1 [Cucurbita moschata])

HSP 1 Score: 1782.7 bits (4616), Expect = 0.0e+00
Identity = 885/985 (89.85%), Postives = 939/985 (95.33%), Query Frame = 0

Query: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
           MVL +KVYLLL + LFAS ANYGFSLYEDQVGLMDWRQQYLGK KHALFHS KSGRKRVV
Sbjct: 1   MVLTVKVYLLLFVTLFASLANYGFSLYEDQVGLMDWRQQYLGKAKHALFHSSKSGRKRVV 60

Query: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
           VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF LGKY++SLSSEGNFLRAWNLPDGQ
Sbjct: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFVLGKYVVSLSSEGNFLRAWNLPDGQ 120

Query: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
           MVWE+F+QGT PSKSLLLVPKSLK N ETVILVFG SCLHAVSSLDGEVIWKIDLTENSV
Sbjct: 121 MVWETFLQGTNPSKSLLLVPKSLKANHETVILVFGSSCLHAVSSLDGEVIWKIDLTENSV 180

Query: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
           EIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGEL+KH T TF+GGFSGELVSVSDDV
Sbjct: 181 EIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGELVKHHTATFSGGFSGELVSVSDDV 240

Query: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
           LVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE SGS+EIVPSKLSG+ A++V S L L
Sbjct: 241 LVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELSGSIEIVPSKLSGLFAVKVDSRLTL 300

Query: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
           +RVKGEGELEVVDKIH QATLSD+LLVSE Q A ALVQ +GS++HL+VKLID+WSS+F++
Sbjct: 301 VRVKGEGELEVVDKIHSQATLSDALLVSEGQLAAALVQHEGSHVHLTVKLIDNWSSNFID 360

Query: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
           ENIVID  RG V KVFL+SYIRTDRSHGFRAL+VMEDHSLLLVQQGE+VWSREDGLASI+
Sbjct: 361 ENIVIDGHRGTVQKVFLHSYIRTDRSHGFRALLVMEDHSLLLVQQGEVVWSREDGLASIV 420

Query: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
           NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS
Sbjct: 421 NVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480

Query: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
           K+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS LL    KSEAC PRWLNIYQWQDP
Sbjct: 481 KMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSRLLQASHKSEACAPRWLNIYQWQDP 540

Query: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
           HHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+EI SSSQ+HSV+QVIPLP TDSTE
Sbjct: 541 HHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGREISSSSQIHSVLQVIPLPFTDSTE 600

Query: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
           QRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWYSVEADSGVIKGH+LKRNCID+ DD
Sbjct: 601 QRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWYSVEADSGVIKGHSLKRNCIDVADD 660

Query: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
           YCFESKDVWSI+LPSESEKII+TA R LNEVVHTQAKV ADQDVMYKYISKNLLFLATVA
Sbjct: 661 YCFESKDVWSIILPSESEKIIATAARKLNEVVHTQAKVVADQDVMYKYISKNLLFLATVA 720

Query: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
           PKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 721 PKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780

Query: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
           HRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 781 HRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840

Query: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
           VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINP+QAEREEGVIPLTDSLPIIPQT
Sbjct: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPTQAEREEGVIPLTDSLPIIPQT 900

Query: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
           YATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLL+T
Sbjct: 901 YATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLIT 960

Query: 961 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALV+AIF TW  SERKELQDKWK
Sbjct: 961 IVALVIAIFATWVFSERKELQDKWK 985

BLAST of Moc08g03360 vs. NCBI nr
Match: XP_023538602.1 (ER membrane protein complex subunit 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1772.3 bits (4589), Expect = 0.0e+00
Identity = 879/985 (89.24%), Postives = 938/985 (95.23%), Query Frame = 0

Query: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
           MVL +KVYLLL + LFAS ANYGFSLYEDQVGLMDWRQQYLGK KHALFHS KSGRKRVV
Sbjct: 1   MVLTVKVYLLLFVTLFASLANYGFSLYEDQVGLMDWRQQYLGKAKHALFHSSKSGRKRVV 60

Query: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
           VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF LGKY++SLSSEGNFLRAWNLPDGQ
Sbjct: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFVLGKYVVSLSSEGNFLRAWNLPDGQ 120

Query: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
           MVWE+F+QGT PSKSLLLVPKSLK NQETVILVFG +CLHAVSSLDGEVIWKIDLTENSV
Sbjct: 121 MVWETFLQGTNPSKSLLLVPKSLKANQETVILVFGSNCLHAVSSLDGEVIWKIDLTENSV 180

Query: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
           EIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGEL+KH T TF+GGFSGELVSVSDDV
Sbjct: 181 EIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGELVKHHTATFSGGFSGELVSVSDDV 240

Query: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
           LVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+D  SGS+EIVPSKLSG+ A++V S L L
Sbjct: 241 LVTLDATKSNLVIINLRNGEIGILQTPIAHLIDVLSGSIEIVPSKLSGLFAVKVDSRLTL 300

Query: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
           +RVKGEGELEVVDKIH QATLS++LLVSE Q A ALVQ +GS++ L+VKLID+WSS+F++
Sbjct: 301 VRVKGEGELEVVDKIHSQATLSEALLVSEGQHAAALVQHEGSHVRLTVKLIDNWSSNFID 360

Query: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
           ENIVID  RG V KVFL+SYIRTDRSHGFRAL+VMEDHSLLLVQQGE+VWSREDGLASI+
Sbjct: 361 ENIVIDGHRGTVQKVFLHSYIRTDRSHGFRALLVMEDHSLLLVQQGEVVWSREDGLASIV 420

Query: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
           NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKS+DKS
Sbjct: 421 NVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSTDKS 480

Query: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
           K+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS LL    KSEAC PRWLNIYQWQDP
Sbjct: 481 KMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSRLLQASHKSEACPPRWLNIYQWQDP 540

Query: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
           HHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+EI SSSQ+HSV+QVIPLP TDSTE
Sbjct: 541 HHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGREIRSSSQIHSVLQVIPLPFTDSTE 600

Query: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
           QRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWYSVEADSGVIKGH+LKRNCID+ DD
Sbjct: 601 QRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWYSVEADSGVIKGHSLKRNCIDVADD 660

Query: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
           YCFESKDVWSI+LPSE+EKII+TA R LNEVVHTQAKV ADQDVMYKYISKNLLFLATVA
Sbjct: 661 YCFESKDVWSIILPSETEKIIATAARKLNEVVHTQAKVVADQDVMYKYISKNLLFLATVA 720

Query: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
           PKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 721 PKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780

Query: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
           HRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 781 HRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840

Query: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
           VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINP+QAE+EEGVIPLTDSLPIIPQT
Sbjct: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPTQAEKEEGVIPLTDSLPIIPQT 900

Query: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
           YATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLL+T
Sbjct: 901 YATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLIT 960

Query: 961 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALV+AIF TW  SERKELQDKWK
Sbjct: 961 IVALVIAIFATWVFSERKELQDKWK 985

BLAST of Moc08g03360 vs. ExPASy Swiss-Prot
Match: Q5ZL00 (ER membrane protein complex subunit 1 OS=Gallus gallus OX=9031 GN=EMC1 PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 8.2e-108
Identity = 294/1037 (28.35%), Postives = 504/1037 (48.60%), Query Frame = 0

Query: 15  LFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLR 74
           L+A       ++YEDQVG  DWRQQY+GK+K A   + + G K+++V TE+NV+A+L+ R
Sbjct: 5   LWALLLPLAAAVYEDQVGKFDWRQQYVGKLKFASLEAAQ-GSKKLLVGTEKNVVAALNSR 64

Query: 75  HGEIFWRHV--LGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQMVWESFIQ-GTK 134
            GEI WRH     P  +ID +    G+  I++SS G  LR+W    G + WE+ +  G+ 
Sbjct: 65  SGEILWRHADKATPEGAIDAM-LIHGQDAITVSSAGRILRSWETNIGGLNWETSLDTGSF 124

Query: 135 PSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDS 194
            + SL+ +  ++K      +L      LH +S  +G   W   L E+     Q++    +
Sbjct: 125 QTASLVGLQDAVK---YVAVLKKAAISLHYLS--NGHQKWVEHLPESENTQYQMLYSRGA 184

Query: 195 DIIYAVGFSSPTQFDQFQINIKSGELIKHS--TETFTGGFSGELVSVSDDVLVTLDATRS 254
            +I+ +G    +      ++++ GE+I+ +     +    +G    V + VLV  D    
Sbjct: 185 GVIHVLGVVPQSHLKVLTLSVEDGEVIEQTKVAAPWLKSLNGACGVVGEAVLVCADTATH 244

Query: 255 NLVILSLRDGEIGIQQSPIAHLVDEFSGSVE----------IVPSKLSGILAIEVG---- 314
           +L + SL + E  ++Q P+  L  EF+   +          I  S+    L +  G    
Sbjct: 245 SLYVCSL-ETEQEMKQIPLQSLDLEFADGFQPRVLATQPSVINASRTQFFLQLSPGHFSL 304

Query: 315 ----------------SVLILIRVKGEGELEVV------------DKIHGQATLSDSLLV 374
                           + L+     GE  +  V            D +HG A       +
Sbjct: 305 LQCKQGLLSHLRDFQQAALVSFATTGEKTVAAVLTCRNELKPASSDGLHGNA-------L 364

Query: 375 SESQRAVALVQRKGSY------MHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYI 434
            +SQ+  AL     +Y      +    +L+D+  +  LE+N    +Q      +++  ++
Sbjct: 365 EDSQKQEALTCSNQTYNINLYLVETGQRLLDTTITFNLEQNGAKPEQ------LYIQVFL 424

Query: 435 RTDRSHGFRALIVMEDHSLLLVQQ-GEIVWSREDGLASIINVVTSELPVEKKGVSIAKVE 494
           + D S G+RAL+  EDH L+ +QQ G++VWSRE+ LA ++++   +LP+      +    
Sbjct: 425 KKDDSVGYRALVQTEDHMLMFLQQPGKVVWSREESLAEVVSLEMVDLPLTGAQAELEGEF 484

Query: 495 NNLIEWLQGHLLKLKGTLMIASPEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFR 554
               + L G  LK   + +I      A +  M     + +S  K++     L+RD    +
Sbjct: 485 GKKADGLLGMFLKRLSSQLILLQAWTAHLWKMFYDARKPRSQIKNEINIDNLARDEFNLQ 544

Query: 555 KLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPS 614
           K+++++T SGKL+ + S  G I+W   L   R   +     L +   +   H       +
Sbjct: 545 KMMVMVTASGKLFGIESSSGTILWKQYLRNVRPGAS-----LKLMVQRTTAHFPHPPQCT 604

Query: 615 VLIVGRCGQSMGGPGFLSFVDTYTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILID 674
           +L+  +  +      FL   +   GK  ++        V+Q + LP+ D    ++ +LID
Sbjct: 605 LLVKDKETKM----SFLYVFNPIFGKRSQVAPPVLKRPVLQTLLLPIMDQDYAKVLLLID 664

Query: 675 AESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDV 734
            E +   +P T   +  L+    +I++Y V+A+ G + G  LK++           +++ 
Sbjct: 665 DEYKVTAFPATKNVLRQLEEMAHSIFFYLVDAEQGKLSGFRLKKD---------LTTEES 724

Query: 735 WSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIG 794
           W + +P+E ++I++   +  +E VH+Q +V  D+ V+YK ++ NLL + T       E  
Sbjct: 725 WQVAIPTEVQRIVTVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVT-------EST 784

Query: 795 TTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVV 854
            T  E +++ +YLID V GRI+H      + GPVH V SENWVVY Y+N KA R E +V+
Sbjct: 785 DTHHERTFVGIYLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVL 844

Query: 855 EIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAISVTSTSKGI 914
           E+Y+ +   N   +       +L  P+     P++L   QSY F  ++ A+  T T +GI
Sbjct: 845 ELYEGTEQYNATAFS------SLDRPL----LPQVL--QQSYIFPSAISAMEATITERGI 904

Query: 915 TSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQV 974
           TS+ LLIG  +  IL+L K  LDPRR   P++  REE +IP +  + I  + +  +   V
Sbjct: 905 TSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTV 964

Query: 975 EGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLTIVALVVAI 986
             +RG+ T P+ LEST L  AYG+D++ TR+ PS+ +D L +D+ Y L+   +  LV A 
Sbjct: 965 SQIRGIYTSPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFAT 983

BLAST of Moc08g03360 vs. ExPASy Swiss-Prot
Match: Q8N766 (ER membrane protein complex subunit 1 OS=Homo sapiens OX=9606 GN=EMC1 PE=1 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.2e-100
Identity = 291/1031 (28.23%), Postives = 497/1031 (48.21%), Query Frame = 0

Query: 15  LFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLR 74
           L+A+      ++YEDQVG  DWRQQY+GKVK A       G K++VV+TE+NVIA+L+ R
Sbjct: 11  LWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEF-SPGSKKLVVATEKNVIAALNSR 70

Query: 75  HGEIFWRHV-LGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQMVWE-SFIQGTKP 134
            GEI WRHV  G  +         G+ +I++S+ G  +R+W    G + WE +   G+  
Sbjct: 71  TGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQ 130

Query: 135 SKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSD 194
           +  L+ + +S++      +L      LH +SS  G + W   L E+     Q++  + S 
Sbjct: 131 ALGLVGLQESVR---YIAVLKKTTLALHHLSS--GHLKWVEHLPESDSIHYQMVYSYGSG 190

Query: 195 IIYAVGFSSPTQFDQFQINIKSGELIK--HSTETFTGGFSGELVSVSDDVLVTLDATRSN 254
           +++A+G    +  +  + N++ GE+++    +  +    SG    V + VLV  D +  +
Sbjct: 191 VVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRS 250

Query: 255 LVILSLRDGEIGIQQSPIAHLVDEFSGSVE--IVPSKLSGILAIEVGSVLIL------IR 314
           L  L+L + E  ++Q P+  L  EF    +  ++P++ + + A      L L      + 
Sbjct: 251 LQTLAL-ETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALL 310

Query: 315 VKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLEEN 374
               G L ++      A +S     +  ++ VA V    + +  S    D     F E++
Sbjct: 311 QYHYGTLSLLKNFPQTALVS---FATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKS 370

Query: 375 IVIDDQRGF--------------------------------VHKVFLNSYIRTDRSHGFR 434
              D    F                                  ++++  +++ D S G+R
Sbjct: 371 SSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYR 430

Query: 435 ALIVMEDHSLLLVQQ--GEIV-WSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWL 494
           AL+  EDH LL +QQ  G++V WSRE+ LA ++ +   +LP+      +        + L
Sbjct: 431 ALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGL 490

Query: 495 QGHLLKLKGTLMIASPEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFRKLLIVLT 554
            G  LK   + +I      + +  M     + +S  K++     L+RD    +K+++++T
Sbjct: 491 LGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVT 550

Query: 555 KSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRC 614
            SGKL+ + S  G I+W   L P  K ++     +       PH       P   ++ + 
Sbjct: 551 ASGKLFGIESSSGTILWKQYL-PNVKPDSSFKLMVQRTTAHFPH------PPQCTLLVKD 610

Query: 615 GQSMGGPGFLSFVDTYTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHL 674
            +S  G   L   +   GK  ++        ++Q + LP+ D    ++ +LID E +   
Sbjct: 611 KES--GMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTA 670

Query: 675 YPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPS 734
           +P T   +  L     +I++Y V+A+ G + G+ L+++    L          W + +P 
Sbjct: 671 FPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELS---------WELTIPP 730

Query: 735 ESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDS 794
           E ++I+    +  +E VH+Q +V  D+ V+YK ++ NLL + T       E      E +
Sbjct: 731 EVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVT-------ESTDAHHERT 790

Query: 795 WLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSR 854
           ++ ++LID V GRI+H      + GPVH V SENWVVY Y+N KA R E +V+E+Y+ + 
Sbjct: 791 FIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTE 850

Query: 855 ADNIDVWKLILGKHNLTSPISSYSRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSKQLL 914
                       ++N T+  SS  RP++     QSY F  S+ A+  T T +GITS+ LL
Sbjct: 851 ------------QYNATA-FSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLL 910

Query: 915 IGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGV 974
           IG  +  IL+L K  LDPRR   P++  REE +IP +  + I  + +  +   V  +RG+
Sbjct: 911 IGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGI 970

Query: 975 VTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGL 986
            T P+ LEST L  AYG+D++ TR+ PS+ +D L +D+ Y L+   +  LV A  +T  L
Sbjct: 971 YTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRL 993

BLAST of Moc08g03360 vs. ExPASy Swiss-Prot
Match: Q6NRB9 (ER membrane protein complex subunit 1 OS=Xenopus laevis OX=8355 GN=emc1 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.4e-99
Identity = 284/1034 (27.47%), Postives = 488/1034 (47.20%), Query Frame = 0

Query: 9   LLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVVVSTEENVI 68
           L  L  L AS A  G ++YEDQVG  DWRQ+Y+G++K A   S   G K+++  T++N+I
Sbjct: 5   LCWLSLLLASLALSG-AVYEDQVGKFDWRQEYVGRIKFASLES-GLGAKKLIAVTDKNII 64

Query: 69  ASLDLRHGEIFWRHVLGPNDSIDGIEFAL---GKYIISLSSEGNFLRAWNLPDGQMVWES 128
           A+L+ R G++ WRHV    D+ +G   AL   G+  I++S  G  LR+W    G + WE+
Sbjct: 65  AALNSRTGDLLWRHV--DKDTSEGTVDALMMIGQDAITVSG-GRLLRSWETNIGALNWEA 124

Query: 129 FIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSVEIQQI 188
            ++      S   V  +   +    + V   S L      +G + W   L E+     Q+
Sbjct: 125 ALE----PGSFQAVSFAGSQDTARYVAVLKNSALSLYFLSNGHLKWSESLPESDTVQYQL 184

Query: 189 IQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFT---GGFSGELVSVSDDVLV 248
           +       ++ VG    +       +++ G  I H     T       G    + + VLV
Sbjct: 185 LYSPYKGSVHVVGLVPHSHLTILTFSLEDGS-ISHQVRVLTPWLRTLHGTCGVIGEGVLV 244

Query: 249 TLDATRSNLVILSLRDGE----IGIQQSPIAHLVDEFSGSVEIVPSK-LSGILA---IEV 308
             D   +++ I+SL  GE      +Q   I    D     V   P   + G L+   +++
Sbjct: 245 CGDVPMASVHIVSLLSGEETTRYSVQSLDIELAEDPTQLDVITAPQNGIGGSLSQFFLQI 304

Query: 309 GSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRK-------------- 368
                L+    +G L  + +   Q +L +     E +  VA++Q K              
Sbjct: 305 APRRFLLMHYHDGVLTPL-RDFSQVSLVNFATTGE-KTVVAVMQCKTEGNPKSGAESEYL 364

Query: 369 -------------GSYMHLSVKLIDSWSSDFLEE--NIVIDDQRGFVHKVFLNSYIRTDR 428
                        G    +++ + DS     LE   +  +D         +L +++R D 
Sbjct: 365 TGQNCAQEPWYCPGHTYSINLYMADS-GRRLLETTMSFTLDQICVRPDSFYLQTFLRKDD 424

Query: 429 SHGFRALIVMEDHSLLLVQQ-GEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLI 488
           S G+RAL+  ED+ LL +QQ G+++W RE+ LA ++ + T +LP+      +        
Sbjct: 425 SVGYRALVQTEDNQLLFLQQPGKLIWLREESLADVVTMETVDLPLTGAQAELEGEFGKKA 484

Query: 489 EWLQGHLLKLKGTLMIASPEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFRKLLI 548
           + L G +LK   + +I      A +  M     + +S  +++     L+RD    +K+++
Sbjct: 485 DGLIGMVLKRLSSQLILLQSWSAHLWKMFCDARKPRSQIRNEINVDTLARDDFNLQKMMV 544

Query: 549 VLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIV 608
           ++T SGKL+ + S  G I+W   LH      +       +   +   H       ++L+ 
Sbjct: 545 MVTASGKLFGIESSSGSILWKFYLHGVHPGSS-----FKLLVQRTTAHFPHPPQCTLLVK 604

Query: 609 GRCGQSMGGPGFLSFVDTYTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESR 668
            +  +       +   +   GK  ++        ++Q + LP+ D+   ++ +L+D + +
Sbjct: 605 DKVTEKSA----MYVFNPIFGKLSQLAPPPLQRPILQSLLLPIMDNDYAKVLLLLDDQHK 664

Query: 669 AHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIM 728
              +P T   +  LQ   S I++Y V+ + G + G  L ++           ++++W ++
Sbjct: 665 VIAFPATKYVLQQLQELHSTIFFYLVDVEKGKLSGLRLNKD---------LSTEEIWEVL 724

Query: 729 LPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTP 788
           LP++ ++I     +  NE VH+Q +V  D+ V+YKY++ NLL L T       E   T P
Sbjct: 725 LPADQQRITVVKGKRSNEHVHSQGRVMGDRSVLYKYLNPNLLVLVT-------ESTDTHP 784

Query: 789 EDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYD 848
           E  ++ +YLID V GRI+H      + GPV  + SENWVVY Y+N KA R E++V+E+Y+
Sbjct: 785 ERCFIGIYLIDGVTGRIIHSSVQRRARGPVQIIHSENWVVYQYWNSKARRNELTVLELYE 844

Query: 849 QSRADNIDVWKLILGKHNLTSPISSYSRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSK 908
            +   N             ++  SS  RP +     QSY F  +++A+  T T +GITS+
Sbjct: 845 GTEQYN-------------STNFSSLDRPLLPHVLQQSYIFPSAIRAMQATITERGITSR 904

Query: 909 QLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGL 968
            +LIG  +  IL+L K  LDPRR   P++  REE +IP T  + I  + +  +   +  +
Sbjct: 905 HILIGLPSGAILSLPKALLDPRRPEIPNEYTREENLIPYTPDIQIHAERFINYNQTISRM 964

Query: 969 RGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVT 986
           RG+ T P+ LEST L  AYG+DL+ TR+ PS+ +D L +D+ Y L+   ++ LV A  +T
Sbjct: 965 RGIYTAPSGLESTCLVVAYGLDLYQTRVYPSKQFDVLKDDYDYILISSVLIGLVFATMIT 987

BLAST of Moc08g03360 vs. ExPASy Swiss-Prot
Match: Q5R7K6 (ER membrane protein complex subunit 1 OS=Pongo abelii OX=9601 GN=EMC1 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 4.1e-99
Identity = 289/1031 (28.03%), Postives = 508/1031 (49.27%), Query Frame = 0

Query: 25  SLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHV- 84
           ++YEDQVG  DWRQQY+GK+K A       G K++VV+TE+NVIA+L+ R GEI WRHV 
Sbjct: 21  AVYEDQVGKFDWRQQYVGKLKFASLEF-SPGSKKLVVATEKNVIAALNSRTGEILWRHVD 80

Query: 85  LGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQMVWESFIQ-GTKPSKSLLLVPKS 144
            G  +         G+ +I++S+ G  +R+W    G + WE  +  G+  +  L+ + +S
Sbjct: 81  KGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDTGSFQALGLVGLQES 140

Query: 145 LKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSP 204
           ++      +L      LH +SS  G + W   L E+     Q++  + S +++A+G    
Sbjct: 141 VR---YIAVLKKTTLALHHLSS--GHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPF 200

Query: 205 TQFDQFQINIKSGELIK--HSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGE 264
           +  +  + N++ GE+++    +  +    SG    V + VLV  D +  +L  L+L + E
Sbjct: 201 SHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLAL-ETE 260

Query: 265 IGIQQSPIAHLVDEFSGSVE--IVPSKLS-------------------------GILAIE 324
             ++Q P+  L  EF    +  ++P++ +                         GIL++ 
Sbjct: 261 WELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGILSLL 320

Query: 325 VG---SVLILIRVKGEGELEVV----DKIHGQATLSDSLLVSESQRAV---ALVQRKGSY 384
                + L+     GE  +  V    +++   +   D  + S S+++    +L     +Y
Sbjct: 321 KNFPQTALVSFATTGEKTVAAVMACRNEVQKTSNSEDGSMGSFSEKSSSKDSLACFNQTY 380

Query: 385 ------MHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMED 444
                 +    +L+D+ ++  LE++    +      ++++  +++ D S G+RAL+  ED
Sbjct: 381 TINLYLVETGRRLLDTTTTFSLEQSGTRPE------RLYIQVFLKKDDSVGYRALVQTED 440

Query: 445 HSLLLVQQ--GEIV-WSREDGLASIINVVTSELPVEKKGVSI-------AKVENNLIEWL 504
           H LL +QQ  G++V WSRE+ LA ++ +   +LP+      +       A +++ L+   
Sbjct: 441 HLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLL--- 500

Query: 505 QGHLLKLKGTLMIASPEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFRKLLIVLT 564
            G  LK   + +I      + +  M     + +S  K++     L+RD    +K+++++T
Sbjct: 501 -GMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVT 560

Query: 565 KSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRC 624
            SGKL+ + S  G I+W   L P  K ++     +       PH       P   ++ + 
Sbjct: 561 ASGKLFGIESSSGTILWKQYL-PSVKPDSSFKLMVQRTTAHFPH------PPQCTLLVKD 620

Query: 625 GQSMGGPGFLSFVDTYTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHL 684
            +S  G   L   +   GK  ++        ++Q + LP+ D    ++ +LID E +   
Sbjct: 621 KES--GMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTA 680

Query: 685 YPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPS 744
           +P T   +  L     +I++Y V+A+ G + G+ L+++    L          W + +P 
Sbjct: 681 FPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELS---------WELTIPP 740

Query: 745 ESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDS 804
           E  +I+    +  +E VH+Q +V  D+ V+YK ++ NLL + T       E      E +
Sbjct: 741 EVRRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVT-------ESTDAHHERT 800

Query: 805 WLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSR 864
           ++ ++LID V GRI+H      + GPVH V SENWVVY Y+N KA R E +V+E+Y+ + 
Sbjct: 801 FIGIFLIDGVTGRIIHSSAQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTE 860

Query: 865 ADNIDVWKLILGKHNLTSPISSYSRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSKQLL 924
                       ++N T+  SS  RP++     QSY F  S+ A+  T T +GITS+ LL
Sbjct: 861 ------------QYNATA-FSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLL 920

Query: 925 IGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGV 984
           IG  +  IL+L K  LDPRR   P++  REE +IP +  + I  + +  +   V  +RG+
Sbjct: 921 IGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGI 980

Query: 985 VTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGL 986
            T P+ LEST L  AYG+D++ TR+ PS+ +D L +D+ Y L+   +  LV A  +T  L
Sbjct: 981 YTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRL 996

BLAST of Moc08g03360 vs. ExPASy Swiss-Prot
Match: Q8C7X2 (ER membrane protein complex subunit 1 OS=Mus musculus OX=10090 GN=Emc1 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.8e-97
Identity = 284/1029 (27.60%), Postives = 496/1029 (48.20%), Query Frame = 0

Query: 25  SLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHV- 84
           ++YEDQVG  DWRQQY+GK+K A       G K++VV+TE+NVIA+L+ R GEI WRHV 
Sbjct: 22  AVYEDQVGKFDWRQQYVGKIKFASLEF-SPGSKKLVVATEKNVIAALNSRTGEILWRHVD 81

Query: 85  LGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQMVWESFIQ-GTKPSKSLLLVPKS 144
            G  +         G+  I++S+ G  +R+W    G + WE  +  G+  +  L+ + +S
Sbjct: 82  KGTAEGAVDAMLVHGQDAITVSNGGRLMRSWETNIGGLNWEITLDTGSFQALGLVGLQES 141

Query: 145 LKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSP 204
           ++      +L      LH +SS  G + W   L E+   + Q++  + S +++A+G    
Sbjct: 142 VR---YIAVLKKTTLTLHHLSS--GHLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPF 201

Query: 205 TQFDQFQINIKSGELIKHST--ETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGE 264
           +  +  + N++ GE+++       +    +G    V + VLV  D +  +L  L+L + E
Sbjct: 202 SHVNIVKFNVEDGEIVQQVRVWTPWLQHLTGACGVVDEAVLVCPDPSSHSLHTLAL-ETE 261

Query: 265 IGIQQSPIAHLVDEFSGSV--EIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQ 324
             ++Q P+     EF      +++P++ S +        L L          ++   HG 
Sbjct: 262 WELRQIPLQSPDLEFGSGFQPQVLPTQPSPVAPSRAQFFLQL----SPSHYALLHYHHGA 321

Query: 325 ATL----SDSLLVS---ESQRAVALVQRKGSYMHLSVKLIDSWSSDFLEENIVID----- 384
            TL      + LVS     ++ VA V    + +   V   D   + F E +   D     
Sbjct: 322 VTLLKNFPQATLVSFATTGEKTVAAVMTCRTEVQKPVSAGDGSVASFPETSGAQDSLACF 381

Query: 385 --------------------------DQRGF-VHKVFLNSYIRTDRSHGFRALIVMEDHS 444
                                     +Q+G    ++++  +++ D S G+RAL+  +DH 
Sbjct: 382 NQTYTINLYLVETGRRLLDTSISFSLEQKGTRPEQLYIQVFLKKDDSVGYRALVQTQDHL 441

Query: 445 LLLVQQ--GEIV-WSREDGLASIINVVTSELPVEKKGVSI-------AKVENNLIEWLQG 504
            L +QQ  G++V WSRE+ LA ++ +   +LP+      +       A +++ L+    G
Sbjct: 442 QLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLL----G 501

Query: 505 HLLKLKGTLMIASPEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFRKLLIVLTKS 564
             LK   + +I      + +  M     + +S  K++     L+RD    +K+++ +T S
Sbjct: 502 MFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVTVTAS 561

Query: 565 GKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQ 624
           GKL+ + S  G I+W   L P  K ++     +       PH       P   ++ +  +
Sbjct: 562 GKLFGIESSSGTILWKQYL-PNVKPDSSFKLMVQRTTAHFPH------PPQCTLLVKDKE 621

Query: 625 SMGGPGFLSFVDTYTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYP 684
           +  G   L   +   GK  ++        ++Q + LP+ D    ++ +L+D E +   +P
Sbjct: 622 T--GMSSLFVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLVDDEYKVTAFP 681

Query: 685 QTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSES 744
            T   +  L     +I++Y V+A+ G + G+ L+++    L          W + +P E 
Sbjct: 682 ATRNVLRQLHELAPSIFFYLVDAEQGRLSGYQLRKDLTTELS---------WELTIPPEV 741

Query: 745 EKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWL 804
           ++++    +  +E VH+Q +V  D+ V+YK ++ NLL + T       E      E +++
Sbjct: 742 QRVVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVT-------ESTDVHHERTFI 801

Query: 805 VVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRAD 864
            ++LID V GRI+H      + GPVH V SENWVVY Y+N KA R E++ +E+Y+ +   
Sbjct: 802 GIFLIDGVTGRIIHSSVQKKARGPVHLVHSENWVVYQYWNSKARRNELTALELYEGTE-- 861

Query: 865 NIDVWKLILGKHNLTSPISSYSRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSKQLLIG 924
                     ++N T+  SS  RP++     QSY F  S+ A+  T T +GITS+ LLIG
Sbjct: 862 ----------QYNATA-FSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIG 921

Query: 925 TINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT 984
             +  IL+L K  LDPRR   P++  REE +IP +  + +  + +  +   V  +RG+ T
Sbjct: 922 LPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQVHAERFINYNQTVSRMRGIYT 981

Query: 985 LPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSE 986
            P+ LEST L  AYG+D++ TR+ PS+ +D L +D+ Y L+   +  LV A  +T  L++
Sbjct: 982 APSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQ 997

BLAST of Moc08g03360 vs. ExPASy TrEMBL
Match: A0A6J1BQZ1 (ER membrane protein complex subunit 1 OS=Momordica charantia OX=3673 GN=LOC111004927 PE=3 SV=1)

HSP 1 Score: 1936.8 bits (5016), Expect = 0.0e+00
Identity = 985/985 (100.00%), Postives = 985/985 (100.00%), Query Frame = 0

Query: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
           MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV
Sbjct: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60

Query: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
           VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ
Sbjct: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120

Query: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
           MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV
Sbjct: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180

Query: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
           EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV
Sbjct: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240

Query: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
           LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL
Sbjct: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300

Query: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
           IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE
Sbjct: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360

Query: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
           ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII
Sbjct: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420

Query: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
           NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS
Sbjct: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480

Query: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
           KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP
Sbjct: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540

Query: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
           HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE
Sbjct: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600

Query: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
           QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD
Sbjct: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660

Query: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
           YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA
Sbjct: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720

Query: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
           PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780

Query: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
           HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840

Query: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
           VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT
Sbjct: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900

Query: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
           YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT
Sbjct: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960

Query: 961 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALVVAIFVTWGLSERKELQDKWK
Sbjct: 961 IVALVVAIFVTWGLSERKELQDKWK 985

BLAST of Moc08g03360 vs. ExPASy TrEMBL
Match: A0A6J1BQR7 (ER membrane protein complex subunit 1 OS=Momordica charantia OX=3673 GN=LOC111004927 PE=3 SV=1)

HSP 1 Score: 1871.3 bits (4846), Expect = 0.0e+00
Identity = 949/949 (100.00%), Postives = 949/949 (100.00%), Query Frame = 0

Query: 37  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 96
           RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA
Sbjct: 21  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 80

Query: 97  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 156
           LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR
Sbjct: 81  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 140

Query: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 216
           SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE
Sbjct: 141 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 200

Query: 217 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 276
           LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS
Sbjct: 201 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 260

Query: 277 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 336
           GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL
Sbjct: 261 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 320

Query: 337 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 396
           VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME
Sbjct: 321 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 380

Query: 397 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 456
           DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG
Sbjct: 381 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 440

Query: 457 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 516
           TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL
Sbjct: 441 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 500

Query: 517 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 576
           LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK
Sbjct: 501 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 560

Query: 577 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 636
           EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY
Sbjct: 561 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 620

Query: 637 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 696
           SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA
Sbjct: 621 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 680

Query: 697 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 756
           KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH
Sbjct: 681 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 740

Query: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 816
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS
Sbjct: 741 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 800

Query: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 801 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 860

Query: 877 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 936
           NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF
Sbjct: 861 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 920

Query: 937 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 986
           TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK
Sbjct: 921 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 969

BLAST of Moc08g03360 vs. ExPASy TrEMBL
Match: A0A6J1GHT1 (ER membrane protein complex subunit 1 OS=Cucurbita moschata OX=3662 GN=LOC111454284 PE=3 SV=1)

HSP 1 Score: 1782.7 bits (4616), Expect = 0.0e+00
Identity = 885/985 (89.85%), Postives = 939/985 (95.33%), Query Frame = 0

Query: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
           MVL +KVYLLL + LFAS ANYGFSLYEDQVGLMDWRQQYLGK KHALFHS KSGRKRVV
Sbjct: 1   MVLTVKVYLLLFVTLFASLANYGFSLYEDQVGLMDWRQQYLGKAKHALFHSSKSGRKRVV 60

Query: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
           VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF LGKY++SLSSEGNFLRAWNLPDGQ
Sbjct: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFVLGKYVVSLSSEGNFLRAWNLPDGQ 120

Query: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
           MVWE+F+QGT PSKSLLLVPKSLK N ETVILVFG SCLHAVSSLDGEVIWKIDLTENSV
Sbjct: 121 MVWETFLQGTNPSKSLLLVPKSLKANHETVILVFGSSCLHAVSSLDGEVIWKIDLTENSV 180

Query: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
           EIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGEL+KH T TF+GGFSGELVSVSDDV
Sbjct: 181 EIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGELVKHHTATFSGGFSGELVSVSDDV 240

Query: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
           LVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE SGS+EIVPSKLSG+ A++V S L L
Sbjct: 241 LVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELSGSIEIVPSKLSGLFAVKVDSRLTL 300

Query: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
           +RVKGEGELEVVDKIH QATLSD+LLVSE Q A ALVQ +GS++HL+VKLID+WSS+F++
Sbjct: 301 VRVKGEGELEVVDKIHSQATLSDALLVSEGQLAAALVQHEGSHVHLTVKLIDNWSSNFID 360

Query: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
           ENIVID  RG V KVFL+SYIRTDRSHGFRAL+VMEDHSLLLVQQGE+VWSREDGLASI+
Sbjct: 361 ENIVIDGHRGTVQKVFLHSYIRTDRSHGFRALLVMEDHSLLLVQQGEVVWSREDGLASIV 420

Query: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
           NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS
Sbjct: 421 NVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480

Query: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
           K+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS LL    KSEAC PRWLNIYQWQDP
Sbjct: 481 KMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSRLLQASHKSEACAPRWLNIYQWQDP 540

Query: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
           HHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+EI SSSQ+HSV+QVIPLP TDSTE
Sbjct: 541 HHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGREISSSSQIHSVLQVIPLPFTDSTE 600

Query: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
           QRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWYSVEADSGVIKGH+LKRNCID+ DD
Sbjct: 601 QRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWYSVEADSGVIKGHSLKRNCIDVADD 660

Query: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
           YCFESKDVWSI+LPSESEKII+TA R LNEVVHTQAKV ADQDVMYKYISKNLLFLATVA
Sbjct: 661 YCFESKDVWSIILPSESEKIIATAARKLNEVVHTQAKVVADQDVMYKYISKNLLFLATVA 720

Query: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
           PKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 721 PKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780

Query: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
           HRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 781 HRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840

Query: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
           VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINP+QAEREEGVIPLTDSLPIIPQT
Sbjct: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPTQAEREEGVIPLTDSLPIIPQT 900

Query: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
           YATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLL+T
Sbjct: 901 YATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLIT 960

Query: 961 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALV+AIF TW  SERKELQDKWK
Sbjct: 961 IVALVIAIFATWVFSERKELQDKWK 985

BLAST of Moc08g03360 vs. ExPASy TrEMBL
Match: A0A6J1KSW0 (ER membrane protein complex subunit 1 OS=Cucurbita maxima OX=3661 GN=LOC111496131 PE=3 SV=1)

HSP 1 Score: 1770.7 bits (4585), Expect = 0.0e+00
Identity = 879/985 (89.24%), Postives = 935/985 (94.92%), Query Frame = 0

Query: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
           MV  +KVYLLL + LFAS ANYGFSLYEDQVGLMDWRQQYLGK KHALFHS KSGRKRVV
Sbjct: 1   MVFTVKVYLLLFVTLFASLANYGFSLYEDQVGLMDWRQQYLGKAKHALFHSSKSGRKRVV 60

Query: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
           VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF LGKY++SLSSEGNFLRAWNLPDGQ
Sbjct: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFVLGKYVVSLSSEGNFLRAWNLPDGQ 120

Query: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
           MVWESF+QGT PSKSLLLVPKSLK NQETVILVFG SCLHAVSSLDGEVIWKIDLTENSV
Sbjct: 121 MVWESFLQGTNPSKSLLLVPKSLKANQETVILVFGSSCLHAVSSLDGEVIWKIDLTENSV 180

Query: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
           EIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGEL+KH T TF+GGFSGELVSVSDDV
Sbjct: 181 EIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGELVKHHTATFSGGFSGELVSVSDDV 240

Query: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
           LVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE SG +EIV SKLSG+ A++V S L L
Sbjct: 241 LVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELSGLIEIVISKLSGLFAVKVDSRLTL 300

Query: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
           +RVKGEGELEVVDKIH QATLSD+LLVSE Q A ALVQ +GS++ L+VKLID+WSS+F++
Sbjct: 301 VRVKGEGELEVVDKIHSQATLSDALLVSEGQHAAALVQHEGSHVRLTVKLIDNWSSNFID 360

Query: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
           ENIVID  RG V K FL+SYIR DRSHGFRAL+VMEDHSLLLVQQGE+VWSREDGLASI+
Sbjct: 361 ENIVIDGHRGTVQKFFLHSYIRKDRSHGFRALLVMEDHSLLLVQQGEVVWSREDGLASIV 420

Query: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
           NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS
Sbjct: 421 NVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480

Query: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
           K+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS LL P  KSEAC PRWLNIYQWQDP
Sbjct: 481 KMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSRLLQPSHKSEACAPRWLNIYQWQDP 540

Query: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
           HHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+EI SSSQ+HSV+QVIPLP TDSTE
Sbjct: 541 HHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGREISSSSQIHSVLQVIPLPFTDSTE 600

Query: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
           QRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWYSVEADSGVIKGH+LKRNCID++DD
Sbjct: 601 QRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWYSVEADSGVIKGHSLKRNCIDVVDD 660

Query: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
           YCFESKDVWSI+LPSE+EKI +TA R LNEVVHTQAKV ADQDVMYKYISKNLLFLATVA
Sbjct: 661 YCFESKDVWSIILPSETEKITATAERKLNEVVHTQAKVVADQDVMYKYISKNLLFLATVA 720

Query: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
           PKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 721 PKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780

Query: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
           HRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 781 HRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840

Query: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
           VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINP+QAE+EEGVIPLTDSLPIIPQT
Sbjct: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPTQAEKEEGVIPLTDSLPIIPQT 900

Query: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
           YATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLL+T
Sbjct: 901 YATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLIT 960

Query: 961 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALV+AIF TW  SERKELQDKWK
Sbjct: 961 IVALVIAIFATWVFSERKELQDKWK 985

BLAST of Moc08g03360 vs. ExPASy TrEMBL
Match: A0A1S3BCL2 (ER membrane protein complex subunit 1 OS=Cucumis melo OX=3656 GN=LOC103488192 PE=3 SV=1)

HSP 1 Score: 1761.5 bits (4561), Expect = 0.0e+00
Identity = 874/985 (88.73%), Postives = 930/985 (94.42%), Query Frame = 0

Query: 1   MVLAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVV 60
           MVLAIK+  LLLL LF+SFANYGFSLYEDQVGLMDWRQQYLGK KHALFHS KSGRKRVV
Sbjct: 1   MVLAIKLSFLLLLTLFSSFANYGFSLYEDQVGLMDWRQQYLGKAKHALFHSSKSGRKRVV 60

Query: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQ 120
           VSTEENVIASLDLRHGEIFWRHVLGPND IDGIEF LGKY++SLSSEGNFLRAWNLPDGQ
Sbjct: 61  VSTEENVIASLDLRHGEIFWRHVLGPNDPIDGIEFVLGKYVVSLSSEGNFLRAWNLPDGQ 120

Query: 121 MVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSV 180
           M WESF+QGT PSKSLLLVPKSLK NQETVILVF RSCLHAVSSLDGEVIWKIDLTENSV
Sbjct: 121 MAWESFLQGTSPSKSLLLVPKSLKANQETVILVFSRSCLHAVSSLDGEVIWKIDLTENSV 180

Query: 181 EIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDV 240
           EIQ+IIQ HDSDIIYAVGFSSPTQ DQFQIN+KSGEL+KH T TF+GGFSGELVSVSDDV
Sbjct: 181 EIQKIIQLHDSDIIYAVGFSSPTQLDQFQINVKSGELLKHQTATFSGGFSGELVSVSDDV 240

Query: 241 LVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLIL 300
           LVT+D  RSNLVI++ ++GEIGI QSPIA ++DEFSG +EIVPSKLSG+LA++V S+L L
Sbjct: 241 LVTVDTARSNLVIINFKNGEIGILQSPIAPVIDEFSGLMEIVPSKLSGLLAVKVNSLLTL 300

Query: 301 IRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLE 360
           +RVKGEGELEVVDKI GQAT+SD+LLVSE+Q A AL   +GS+MHL+VKLID+WS+ F++
Sbjct: 301 VRVKGEGELEVVDKIPGQATVSDALLVSENQHAAALAHHEGSHMHLTVKLIDNWSTSFID 360

Query: 361 ENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASII 420
           ENIVID QRG V KVFLNSYIRTDRSHGFRAL+VMEDHSLLLVQQGEIVWSREDGLASI+
Sbjct: 361 ENIVIDKQRGSVQKVFLNSYIRTDRSHGFRALLVMEDHSLLLVQQGEIVWSREDGLASIV 420

Query: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKS 480
           NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDV AIQNMRLKSSDKS
Sbjct: 421 NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVVAIQNMRLKSSDKS 480

Query: 481 KLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEACTPRWLNIYQWQDP 540
           K+SRDHNGFRKLLIVLTKSGKL+ALHSGDGR+VWS LL PF KS+ C PRWLNIYQWQDP
Sbjct: 481 KMSRDHNGFRKLLIVLTKSGKLFALHSGDGRVVWSRLLQPFHKSKDCAPRWLNIYQWQDP 540

Query: 541 HHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTE 600
           HHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTGKEI SSSQ HS+V+VIPLP TDSTE
Sbjct: 541 HHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGKEISSSSQTHSIVKVIPLPFTDSTE 600

Query: 601 QRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCIDILDD 660
           +RLHILIDAE RAHLYPQTSEAI ILQSEFSNIYWYSVEADSG+IKGHAL R C+D++DD
Sbjct: 601 RRLHILIDAEGRAHLYPQTSEAIGILQSEFSNIYWYSVEADSGIIKGHALMRKCVDVVDD 660

Query: 661 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 720
           YCFESKDVW IMLPS+SEKII++ATR LNEVVHTQAKV ADQDVMYKYISKNLLFLATVA
Sbjct: 661 YCFESKDVWLIMLPSDSEKIIASATRKLNEVVHTQAKVVADQDVMYKYISKNLLFLATVA 720

Query: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 780
           PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRM HHGSTGPVHAVFSENWVVYHYFNLKA
Sbjct: 721 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMIHHGSTGPVHAVFSENWVVYHYFNLKA 780

Query: 781 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 840
           HRYEMSVVEIYDQSRADNIDVWKLI+GKHNLT+PISSYSRPEILAKSQSYFFTHSVKAIS
Sbjct: 781 HRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTTPISSYSRPEILAKSQSYFFTHSVKAIS 840

Query: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900
           VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT
Sbjct: 841 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 900

Query: 901 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 960
           Y THALQVEGLRG++T+PAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLL+T
Sbjct: 901 YVTHALQVEGLRGIMTIPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLIT 960

Query: 961 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALV+AIF TW LSERKELQDKWK
Sbjct: 961 IVALVIAIFATWVLSERKELQDKWK 985

BLAST of Moc08g03360 vs. TAIR 10
Match: AT5G11560.1 (catalytics )

HSP 1 Score: 1304.3 bits (3374), Expect = 0.0e+00
Identity = 634/985 (64.37%), Postives = 803/985 (81.52%), Query Frame = 0

Query: 3   LAIKVYLLLLLALFASFANYGFSLYEDQVGLMDWRQQYLGKVKHALFHSQKSGRKRVVVS 62
           +AI+V+L LL  LF S A   FSLYEDQ GL DW Q+Y+GKVKHA+FH+QK+GRKRV+VS
Sbjct: 1   MAIRVFLTLL--LFLSSAILSFSLYEDQAGLTDWHQRYIGKVKHAVFHTQKTGRKRVIVS 60

Query: 63  TEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFALGKYIISLSSEGNFLRAWNLPDGQMV 122
           TEENV+ASLDLRHGEIFWRHVLG  D+IDG+  ALGKY+I+LSSEG+ LRAWNLPDGQMV
Sbjct: 61  TEENVVASLDLRHGEIFWRHVLGTKDAIDGVGIALGKYVITLSSEGSTLRAWNLPDGQMV 120

Query: 123 WESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRSCLHAVSSLDGEVIWKIDLTENSVEI 182
           WE+ +   + SKSLL VP +LK +++  I VFG   LHAVS++DGEV+WK D T    E+
Sbjct: 121 WETSLHTAQHSKSLLSVPINLKVDKDYPITVFGGGYLHAVSAIDGEVLWKKDFTAEGFEV 180

Query: 183 QQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGELIKHSTETFTGGFSGELVSVSDDVLV 242
           Q+++Q   S IIY +GF   ++   +QI+ KSGE++   +  F GGFSGE+ SVS D +V
Sbjct: 181 QRVLQAPGSSIIYVLGFLHSSEAVVYQIDSKSGEVVAQKSTVFPGGFSGEISSVSSDKVV 240

Query: 243 TLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSGSVEIVPSKLSGILAIEVGSVLILIR 302
            LD+TRS LV +   DG+I  Q++PI+ LV++ SG+ EI+   LS +LA++V    I + 
Sbjct: 241 VLDSTRSILVTIGFIDGDISFQKTPISDLVED-SGTAEILSPLLSNMLAVKVNKRTIFVN 300

Query: 303 VKGEGELEVVDKIHGQATLSDSLLVSESQRAVALVQRKGSYMHLSVKLIDSWSSDFLEEN 362
           V  +G+LEVVD +  +  +SDSL V++ Q A A V  +GS +HL VKL++  ++  L E 
Sbjct: 301 VGDKGKLEVVDSLSDETAMSDSLPVADDQEAFASVHHEGSRIHLMVKLVNDLNNVLLRET 360

Query: 363 IVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQGEIVWSREDGLASIINV 422
           I +D  RG VHKVF+N+YIRTDRS+GFRALIVMEDHSLLL+QQG IVWSRE+GLAS+ +V
Sbjct: 361 IQMDQNRGRVHKVFMNNYIRTDRSNGFRALIVMEDHSLLLLQQGAIVWSREEGLASVTDV 420

Query: 423 VTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQNMRLKSSDKSKL 482
            T+ELP+EK GVS+AKVE+ L EWL+GH+LKLKG+L++ASPEDV AIQ++R+KSS K+KL
Sbjct: 421 TTAELPLEKDGVSVAKVEHTLFEWLKGHVLKLKGSLLLASPEDVVAIQDLRVKSSGKNKL 480

Query: 483 SRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHPFRKSEAC-TPRWLNIYQWQDPH 542
           +RDHNGFRKL++ LT++GKL+ALH+GDGRIVWS+LL+   +S++C  P  +++YQWQ PH
Sbjct: 481 TRDHNGFRKLILALTRAGKLFALHTGDGRIVWSMLLNSPSQSQSCERPNGVSLYQWQVPH 540

Query: 543 HRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGKEIGSSSQVHSVVQVIPLPLTDSTEQ 602
           H AMDENPSVL+VG+CG     PG LSFVD YTGKEI SS   HSVVQV+PLP+TDS EQ
Sbjct: 541 HHAMDENPSVLVVGKCGSDSSAPGVLSFVDVYTGKEISSSDIGHSVVQVMPLPITDSKEQ 600

Query: 603 RLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSVEADSGVIKGHALKRNCI-DILDD 662
           RLH++ D     HLYP+TSEA+SI Q EF N+YWY+VEAD G+I+GH +K +C  +  D+
Sbjct: 601 RLHLIADTVGHVHLYPKTSEALSIFQREFQNVYWYTVEADDGIIRGHVMKGSCSGETADE 660

Query: 663 YCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKVAADQDVMYKYISKNLLFLATVA 722
           YCF ++++W+++ PSESEKIIST TR  NEVVHTQAKV  DQD++YKY+S+NLLF+ATV+
Sbjct: 661 YCFTTRELWTVVFPSESEKIISTLTRKPNEVVHTQAKVNTDQDLLYKYVSRNLLFVATVS 720

Query: 723 PKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGSTGPVHAVFSENWVVYHYFNLKA 782
           PK +GEIG+ TPE+S LVVYLID + GRILHR++H G  GPVHAVFSENWVVYHYFNL+A
Sbjct: 721 PKGAGEIGSVTPEESSLVVYLIDTITGRILHRLSHQGCQGPVHAVFSENWVVYHYFNLRA 780

Query: 783 HRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSYSRPEILAKSQSYFFTHSVKAIS 842
           H+YE++VVEIYDQSRA+N +VWKLILGKHNLT+PI+SYSRPE+  KSQSYFF  SVK I+
Sbjct: 781 HKYEVTVVEIYDQSRAENKNVWKLILGKHNLTAPITSYSRPEVFTKSQSYFFAQSVKTIA 840

Query: 843 VTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQT 902
           VTST+KGITSKQLLIGTI DQILALDKR++DPRR++NPSQAE+EEG+IPLTD+LPIIPQ 
Sbjct: 841 VTSTAKGITSKQLLIGTIGDQILALDKRFVDPRRTLNPSQAEKEEGIIPLTDTLPIIPQA 900

Query: 903 YATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFFTRITPSRTYDSLTEDFSYALLLLT 962
           Y TH+ +VEGLRG+VT P+KLESTT  FAYGVDLF+TR+ PS+TYDSLT+DFSYALLL+T
Sbjct: 901 YVTHSHKVEGLRGIVTAPSKLESTTHVFAYGVDLFYTRLAPSKTYDSLTDDFSYALLLIT 960

Query: 963 IVALVVAIFVTWGLSERKELQDKWK 986
           IVALV AI++TW LSE+KEL +KW+
Sbjct: 961 IVALVAAIYITWVLSEKKELSEKWR 982