Moc08g03300 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc08g03300 vs. NCBI nr
Match: XP_022131335.1 (protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 566/568 (99.65%), Postives = 566/568 (99.65%), Query Frame = 0

Query: 1   MADSNGAAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYG 60
           MADSNGAAEEI AEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYG
Sbjct: 1   MADSNGAAEEIXAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYG 60

Query: 61  MSALQTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQ 120
           MSALQTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQ
Sbjct: 61  MSALQTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQ 120

Query: 121 ARPYCDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILES 180
           ARPYCDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILES
Sbjct: 121 ARPYCDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILES 180

Query: 181 FIGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPS 240
           FIGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPS
Sbjct: 181 FIGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPS 240

Query: 241 SSLFTSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGED 300
           SSLFTSLFQVVVA YRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGED
Sbjct: 241 SSLFTSLFQVVVAXYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGED 300

Query: 301 EERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNI 360
           EERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNI
Sbjct: 301 EERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNI 360

Query: 361 EIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASI 420
           EIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASI
Sbjct: 361 EIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASI 420

Query: 421 AVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKS 480
           AVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKS
Sbjct: 421 AVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKS 480

Query: 481 MSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGIC 540
           MSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGIC
Sbjct: 481 MSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGIC 540

Query: 541 FIDLLYFVACCRSYDGGDEEDETCKLQD 569
           FIDLLYFVACCRSYDGGDEEDETCKLQD
Sbjct: 541 FIDLLYFVACCRSYDGGDEEDETCKLQD 568

BLAST of Moc08g03300 vs. NCBI nr
Match: KAG2691852.1 (hypothetical protein I3760_08G025600 [Carya illinoinensis] >KAG6698566.1 hypothetical protein I3842_08G025800 [Carya illinoinensis])

HSP 1 Score: 594.3 bits (1531), Expect = 1.1e-165
Identity = 310/569 (54.48%), Postives = 401/569 (70.47%), Query Frame = 0

Query: 7   AAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQT 66
           A E+   +EPLL  G  KGG RTLPFII NE+ E   S GL  N   YL  +YGM     
Sbjct: 5   ADEKDMIKEPLL--GTPKGGFRTLPFIIANEAFERAASVGLLPNMILYLTRDYGMPTPTA 64

Query: 67  SNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCD 126
           +NFL+  S      P+LGAFLADS  GR+R I FG+  + LGM LLW+T+  PQA+  C 
Sbjct: 65  ANFLFLWSAATNFMPILGAFLADSCLGRYRMIGFGSIASFLGMVLLWLTTMTPQAQIPCT 124

Query: 127 KSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESFIG 186
              S C+ PT+FQL +LY  F LM IG GGI++SSLAFG DQL      +N  +L SF  
Sbjct: 125 LGSSSCNFPTSFQLLLLYFSFGLMAIGAGGIRSSSLAFGADQLDKRDNMRNAGMLGSFFS 184

Query: 187 CYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSL 246
            YYV T V +L  +T+IVYIQD + W++GFGIPA LM F   SF L SP ++K++ + SL
Sbjct: 185 WYYVSTSVAALIAVTLIVYIQDTMGWTVGFGIPALLMFFSAFSFFLASPFYLKLKATRSL 244

Query: 247 FTSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACII----TEGE 306
            T L QV+V+SYRNRH++L S  +  +YH    S    PS+KLRFLNKACII     +  
Sbjct: 245 LTGLAQVLVSSYRNRHLKLSSQATAEMYHQMKGSMPLMPSEKLRFLNKACIIRNPLQDLT 304

Query: 307 DEERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPN 366
            E ++ +PW +C V QVEDLK++I++IP+WSTGI+ S  F+QN+ P LQ ++M+RH+TPN
Sbjct: 305 PEGKASEPWSLCTVNQVEDLKALIRIIPLWSTGIMMSAIFSQNSFPVLQAASMNRHVTPN 364

Query: 367 IEIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVAS 426
            EIPAGSF MFL ISL +WI LYD +++PLASKI GKP RL   Q++G G+  SC+SVA+
Sbjct: 365 FEIPAGSFSMFLIISLTIWIALYDLVVLPLASKISGKPVRLGVKQRMGIGLVFSCMSVAA 424

Query: 427 IAVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPK 486
           +AVVE  RR +AI++GFS+E  AVV MSA WLLPHYVFA +A+A   IGQNEF+YSQLPK
Sbjct: 425 LAVVESIRRELAIEEGFSDEPEAVVSMSAMWLLPHYVFAGLAEAFNAIGQNEFYYSQLPK 484

Query: 487 SMSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGI 546
           +MSS++ +L G+ MS G+LVAGF+M+ VDDVT+ GGGESWVSSNINKAHY+YY+ L+ G+
Sbjct: 485 TMSSIAATLFGVGMSVGNLVAGFVMSTVDDVTRKGGGESWVSSNINKAHYDYYYWLLAGL 544

Query: 547 CFIDLLYFVACCRSYDGGDEEDETCKLQD 569
             I+L+YF+ CC++Y  G  E E C + D
Sbjct: 545 GSINLVYFLVCCKAY--GCCEVERCVVSD 569

BLAST of Moc08g03300 vs. NCBI nr
Match: KAG6670683.1 (hypothetical protein I3843_Q045400 [Carya illinoinensis])

HSP 1 Score: 590.5 bits (1521), Expect = 1.5e-164
Identity = 309/569 (54.31%), Postives = 400/569 (70.30%), Query Frame = 0

Query: 7   AAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQT 66
           A E+   +EPLL  G  KGG RTLPFII NE+ E   S GL  N   YL  +YGM     
Sbjct: 5   ADEKDMIKEPLL--GTPKGGFRTLPFIIANEAFERAASVGLLPNMILYLTRDYGMPTPTA 64

Query: 67  SNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCD 126
           +NFL+  S      P+LGAFLADS  GR+R I FG+  + LGM LLW+T+  PQA+  C 
Sbjct: 65  ANFLFLWSAATNFMPILGAFLADSCLGRYRMIGFGSIASFLGMVLLWLTTMTPQAQIPCT 124

Query: 127 KSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESFIG 186
              S C+ PT+FQL +LY  F LM IG GGI++SSLAFG DQL      +N  +L SF  
Sbjct: 125 LGSSSCNFPTSFQLLLLYFSFGLMAIGAGGIRSSSLAFGADQLDKRDNMRNAGMLGSFFS 184

Query: 187 CYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSL 246
            YYV T V +L  +T+IVYIQD + W++GFGIPA LM F   SF L SP ++K++ + SL
Sbjct: 185 WYYVSTSVAALIAVTLIVYIQDTMGWTVGFGIPALLMFFSAFSFFLASPFYLKLKATRSL 244

Query: 247 FTSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACII----TEGE 306
            T L QV+V+S RNRH++L S  +  +YH    S    PS+KLRFLNKACII     +  
Sbjct: 245 LTGLAQVLVSSDRNRHLKLSSQATAEMYHQMKGSMPLMPSEKLRFLNKACIIRNPLQDLT 304

Query: 307 DEERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPN 366
            E ++ +PW +C V QVEDLK++I++IP+WSTGI+ S  F+QN+ P LQ ++M+RH+TPN
Sbjct: 305 PEGKASEPWSLCTVNQVEDLKALIRIIPLWSTGIMMSAIFSQNSFPVLQAASMNRHVTPN 364

Query: 367 IEIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVAS 426
            EIPAGSF MFL ISL +WI LYD +++PLASKI GKP RL   Q++G G+  SC+SVA+
Sbjct: 365 FEIPAGSFSMFLIISLTIWIALYDLVVLPLASKISGKPVRLGVKQRMGIGLVFSCMSVAA 424

Query: 427 IAVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPK 486
           +AVVE  RR +AI++GFS+E  AVV MSA WLLPHYVFA +A+A   IGQNEF+YSQLPK
Sbjct: 425 LAVVESIRRELAIEEGFSDEPEAVVSMSAMWLLPHYVFAGLAEAFNAIGQNEFYYSQLPK 484

Query: 487 SMSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGI 546
           +MSS++ +L G+ MS G+LVAGF+M+ VDDVT+ GGGESWVSSNINKAHY+YY+ L+ G+
Sbjct: 485 TMSSIAATLFGVGMSVGNLVAGFVMSTVDDVTRKGGGESWVSSNINKAHYDYYYWLLAGL 544

Query: 547 CFIDLLYFVACCRSYDGGDEEDETCKLQD 569
             I+L+YF+ CC++Y  G  E E C + D
Sbjct: 545 GSINLVYFLVCCKAY--GCCEVERCVVSD 569

BLAST of Moc08g03300 vs. NCBI nr
Match: XP_042992281.1 (protein NRT1/ PTR FAMILY 1.2-like [Carya illinoinensis] >KAG6643997.1 hypothetical protein CIPAW_08G025100 [Carya illinoinensis])

HSP 1 Score: 590.1 bits (1520), Expect = 2.0e-164
Identity = 308/569 (54.13%), Postives = 400/569 (70.30%), Query Frame = 0

Query: 7   AAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQT 66
           A E+   +EPLL  G  KGG RTLPFII NE+ E   S GL  N   YL  +YGM     
Sbjct: 5   ADEKDMIKEPLL--GTPKGGFRTLPFIIANEAFERAASVGLLPNMILYLTRDYGMPTPTA 64

Query: 67  SNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCD 126
           +NFL+  S      P+LGAFLADS  GR+R I FG+  + LGM LLW+T+  PQA+  C 
Sbjct: 65  ANFLFLWSAATNFMPILGAFLADSCLGRYRMIGFGSIASFLGMVLLWLTTMTPQAQIPCT 124

Query: 127 KSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESFIG 186
              S C+ PT+FQL +LY  F LM IG GGI++SSLAFG DQL      +N  +L SF  
Sbjct: 125 LGSSSCNFPTSFQLLLLYFSFGLMAIGAGGIRSSSLAFGADQLDKRDNMRNAGMLGSFFS 184

Query: 187 CYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSL 246
            YYV T V +L  +T+IVYIQD + W++GFGIPA LM F   SF L SP ++K++ + SL
Sbjct: 185 WYYVSTSVAALIAVTLIVYIQDTMGWTVGFGIPALLMFFSAFSFFLASPFYLKLKATRSL 244

Query: 247 FTSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACII----TEGE 306
            T L QV+V+SYRNRH++L S  +  +YH    S    PS+KLRFLNKACII     +  
Sbjct: 245 LTGLAQVLVSSYRNRHLKLSSQATAEMYHQMKGSMPLMPSEKLRFLNKACIIRNPLQDLT 304

Query: 307 DEERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPN 366
            E ++ +PW +C V QVEDLK+++++IP+WSTGI+ S  F+QN+ P LQ ++M+RH+TPN
Sbjct: 305 PEGKASEPWSLCTVNQVEDLKALMRIIPLWSTGIMMSAIFSQNSFPVLQAASMNRHVTPN 364

Query: 367 IEIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVAS 426
            EIPAGSF MFL ISL +WI LYD +++PLASKI GKP RL   Q++G G+  SC+SVA+
Sbjct: 365 FEIPAGSFSMFLIISLTIWIALYDLVVLPLASKISGKPVRLGVKQRMGIGLVFSCMSVAA 424

Query: 427 IAVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPK 486
           +AVVE  RR +AI++GFS+E  AVV MSA WLLP YVFA +A+A   IGQNEF+YSQLPK
Sbjct: 425 LAVVESIRRELAIEEGFSDEPEAVVSMSAMWLLPRYVFAGLAEAFNAIGQNEFYYSQLPK 484

Query: 487 SMSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGI 546
           +MSS++ +L G+ MS G+LVAGF+M+ VDDVT+ GGGESWVSSNINKAHY+YY+ L+ G+
Sbjct: 485 TMSSIAATLFGVGMSVGNLVAGFVMSTVDDVTRKGGGESWVSSNINKAHYDYYYWLLAGL 544

Query: 547 CFIDLLYFVACCRSYDGGDEEDETCKLQD 569
             I+L+YF+ CC++Y  G  E E C + D
Sbjct: 545 GSINLVYFLVCCKAY--GCCEVERCVVSD 569

BLAST of Moc08g03300 vs. NCBI nr
Match: XP_030947173.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Quercus lobata])

HSP 1 Score: 570.5 bits (1469), Expect = 1.7e-158
Identity = 295/563 (52.40%), Postives = 391/563 (69.45%), Query Frame = 0

Query: 5   NGAAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSAL 64
           N + E+   +EPLL     KGG +TLPFII NE+ E   S GL  N   YL  E+GM   
Sbjct: 3   NPSDEKDMVKEPLLNTYKTKGGFKTLPFIIANEAFEGVASFGLMPNMILYLTREFGMQIA 62

Query: 65  QTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPY 124
             +N L+  S      P+LGA+LADSY GR+R I FG+  + LGM LLW+T+   QA+  
Sbjct: 63  SAANVLFIWSAATNFMPILGAYLADSYVGRYRMIGFGSIVSLLGMVLLWLTTLNSQAKIS 122

Query: 125 CDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESF 184
           C +S S C+  TAF+L  LYS F LM IG GG+++SSLAFG DQL      +N  +LESF
Sbjct: 123 CSQSSSSCNFSTAFKLLHLYSSFGLMAIGAGGLRSSSLAFGADQLDKRDNIRNAAMLESF 182

Query: 185 IGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSS 244
              YYV     +L  +T IVY+QD + W +GFGIPA LM   ++SF L SP ++K++  S
Sbjct: 183 FSWYYVSKTASALIAVTFIVYVQDKMGWMVGFGIPALLMSLSVLSFFLASPFYIKLKSDS 242

Query: 245 SLFTSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACII----TE 304
           SL T   QV+VASY+NR  RL S  +  +YHH + S    PS+KLRFLNKACII     +
Sbjct: 243 SLLTGFTQVLVASYKNRRTRLSSQATPEMYHHINGSMLLLPSEKLRFLNKACIIKNPLQD 302

Query: 305 GEDEERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHIT 364
              + R+ DPW +C V QVE+LK++I+VIP+WS GI+ SVS +QN+ P LQ ++MDRHIT
Sbjct: 303 LSPDGRATDPWSLCTVNQVEELKALIRVIPLWSAGIMMSVSISQNSFPVLQAASMDRHIT 362

Query: 365 PNIEIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISV 424
           P  EIPAGSF +FL I++ LWI LYDR+++PLASKI GKP RL   +++G G+F SC+S+
Sbjct: 363 PYFEIPAGSFSVFLVITITLWITLYDRVVLPLASKISGKPCRLNEKKRMGIGLFFSCMSM 422

Query: 425 ASIAVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQL 484
           A++AV E  RR +AI++GFS++  AVV MSA WLLP+Y+FA + +A   IGQNEF+YSQL
Sbjct: 423 AAMAVAESVRRELAIEEGFSDDPQAVVSMSAMWLLPYYIFAGLGEAFNAIGQNEFYYSQL 482

Query: 485 PKSMSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLIC 544
           PKSMSS++T+L GL MSA SLVA FI++ VD+VTK  GGESWVSSNINKAHY+YY+ L+ 
Sbjct: 483 PKSMSSIATTLFGLGMSAASLVASFILSTVDNVTKK-GGESWVSSNINKAHYDYYYWLLT 542

Query: 545 GICFIDLLYFVACCRSYDGGDEE 561
           G+C  +L+Y++ C ++Y   + E
Sbjct: 543 GLCSANLMYYLFCSKAYGPSEGE 564

BLAST of Moc08g03300 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.7e-137
Identity = 262/549 (47.72%), Postives = 362/549 (65.94%), Query Frame = 0

Query: 20  GGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFRSTLGGL 79
           G   KGGI T+PFII NE+ E   S GL  N   YL+ +Y     + +N L+  S     
Sbjct: 18  GKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNF 77

Query: 80  TPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPY-CDKSL--SRCDSPT 139
           TP+LGAFL+DSY GRF TI+  +  + LGM LLW+T+ +PQ +P  CD +   S C S T
Sbjct: 78  TPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSST 137

Query: 140 AFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESFIGCYYVFTLVGS 199
           A QL +LYS F L+ IG GGI+  SLAFG DQL      KN+ +LESF G YY  + V  
Sbjct: 138 ASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAV 197

Query: 200 LFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQVVVA 259
           L   T IVYIQ+++ W +GFG+PA LML   + F+L SPL+V    + SLFT L Q +VA
Sbjct: 198 LIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVA 257

Query: 260 SYRNRHVRLPS-DTSHALYHHGDASARQKPSDKLRFLNKACIITEGEDEERSG----DPW 319
           +Y+ R + LP    S   Y+H   S  + PS KLRFLNKAC+I+  E+E  S     +PW
Sbjct: 258 AYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPW 317

Query: 320 RVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHIT---PNIEIPAG 379
           R+C  ++VE+LK++IKVIPIWSTGI+ S++ +Q++   LQ ++MDR ++    + ++PAG
Sbjct: 318 RLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAG 377

Query: 380 SFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEI 439
           SF MF  I+L LW++LYDR ++PLASKI G+P RL    ++G G+FMS +++A  A+VE 
Sbjct: 378 SFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVES 437

Query: 440 KRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVS 499
            RR  AI QG++N S+AVV +SA WL+P YV   +A+A   IGQ EFFY++ PKSMSS++
Sbjct: 438 FRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIA 497

Query: 500 TSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLL 555
            SL GL M+  SL+A  ++  V+++T   G ESWVS NINK HY YY+ ++  + FI+++
Sbjct: 498 ASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVI 557

BLAST of Moc08g03300 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 456.1 bits (1172), Expect = 6.0e-127
Identity = 247/544 (45.40%), Postives = 347/544 (63.79%), Query Frame = 0

Query: 24  KGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFRSTLGGLTPVL 83
           KGG+ T+PFII NE  E   S GL  N   YLM +Y +  ++    L+         P++
Sbjct: 24  KGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLV 83

Query: 84  GAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPY-CDKSL-SRCDSPTAFQLF 143
           GAFL+DSY GRF TI   +  + LGM +LW+T+ +PQ +P  C  +  + C S T+ QL 
Sbjct: 84  GAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLA 143

Query: 144 VLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESFIGCYYVFTLVGSLFGMT 203
           +LY+ F L+ IG GGI+  SLAFG DQL      KN+ +LESF G YY  + V  L   T
Sbjct: 144 LLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFT 203

Query: 204 VIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQVVVASYRNR 263
           VIVYIQD++ W +GFGIPA LML     F+  SPL+VK + S SLFT L QVV A+Y  R
Sbjct: 204 VIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKR 263

Query: 264 HVRLPS-DTSHALYHHGDASARQKPSDKLRFLNKACIITEGEDEERSGD-----PWRVCR 323
           ++ LP    S   Y+    S  + PSDKLRFLNKAC I+   DE+   D      WR+C 
Sbjct: 264 NLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAIS-NRDEDLGSDGLALNQWRLCT 323

Query: 324 VEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPN--IEIPAGSFIMF 383
            +QVE LK+++KVIP+WSTGI+ S++ +QN+   LQ  +MDR ++ N   +IPAGSF MF
Sbjct: 324 TDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMF 383

Query: 384 LFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEIKRRAM 443
             I+L  W++LYDR ++PLASKI G+P R+    ++G G+F+S +++A  A VE  RR  
Sbjct: 384 TIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKT 443

Query: 444 AIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVSTSLSG 503
           AI QG +N++++ V +SA WL+P YV   +A+A   IGQ EFFY++ PKSMSS++ SL G
Sbjct: 444 AISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFG 503

Query: 504 LSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLLYFVAC 555
           L M+  +++A  I+  V + +K  G  SW+  NINK HY+YY+ ++  + F++++Y+V C
Sbjct: 504 LGMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVC 563

BLAST of Moc08g03300 vs. ExPASy Swiss-Prot
Match: Q9LYD5 (Protein NRT1/ PTR FAMILY 1.3 OS=Arabidopsis thaliana OX=3702 GN=NPF1.3 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 9.9e-90
Identity = 204/547 (37.29%), Postives = 296/547 (54.11%), Query Frame = 0

Query: 14  EEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFR 73
           E  L+G    K GI T+PFI+ ++++E     GL  N   +L  EYGM   + +N L+  
Sbjct: 4   EALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILFLW 63

Query: 74  STLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCDKSLSRCD 133
           S      P++GAF+ADSY GRF  I FG++ +  GM LLW+T+ I   RP CDK  + C 
Sbjct: 64  SAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNVCQ 123

Query: 134 SPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHI----LESFIGCYYVFT 193
             T  +  +LYS F L  IG GG+++S LAF  DQL  N+   +    LE+    YY   
Sbjct: 124 PTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYFSV 183

Query: 194 LVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQ 253
           +V      +++V++Q    W +GFG+  A M   +  F   SP +V+             
Sbjct: 184 MVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYVRF------------ 243

Query: 254 VVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGEDEERSGDPWR 313
                                         QKP+                    S +PW+
Sbjct: 244 ------------------------------QKPT------------------RNSRNPWK 303

Query: 314 VCRVEQVEDLKSIIKVIPIWSTGILFS-VSFNQNTLPYLQVSTMDRH-ITPNIEIPAGSF 373
           +CRV+QVEDLKS+I VIPIWSTGI+ S V+  Q +   LQ  TMDRH      EIP GS+
Sbjct: 304 LCRVQQVEDLKSLINVIPIWSTGIILSLVTACQVSFIVLQAKTMDRHTFIQGFEIPPGSY 363

Query: 374 IMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEIKR 433
            +FL IS  L++ LYD ++VPL S  L +P RL  + ++  G  +S + ++++A  E  R
Sbjct: 364 GIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISALAATEYAR 423

Query: 434 RAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVSTS 493
           R  A          +  K+SA WLLP+ +   IA+A   I QNEFFYS+LPK+MSSV+T+
Sbjct: 424 RKTA-------RDESGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKTMSSVATT 476

Query: 494 LSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLLYF 553
           LS L+M+A SL++ +I+T+V DVT  G   SW++ NI++ H +YY+ L+ G+  +++LYF
Sbjct: 484 LSSLNMAAASLISSWIITIV-DVTTYG---SWITENIDEGHLDYYYWLLVGLSLLNVLYF 476

Query: 554 VACCRSY 555
           V C +SY
Sbjct: 544 VWCKKSY 476

BLAST of Moc08g03300 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.2e-88
Identity = 186/544 (34.19%), Postives = 300/544 (55.15%), Query Frame = 0

Query: 23  KKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFRSTLGGLTPV 82
           K GG R + FI+GNE++E   S GL +NF  YL   + +  +  +N +   S    LTP+
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 83  LGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCDKSLS--RCDSPTAFQL 142
           +GA+++D+Y GRF+TIAF +  T LG+  + +T++ PQ  P    S     C  P   Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 143 FVLYSCFVLMCIGGGGIKASSLAFGGDQL--ATNKNKHILESFIGCYYVFTLVGSLFGMT 202
            VL      + +G GGI+  S+ FG DQ    T +    + SF   YY+   V  +   T
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 230

Query: 203 VIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQVVVASYRNR 262
           V+VYIQD +SW +GF IP  LM   +V F      +V ++P  S+F+ + QV+VA+ + R
Sbjct: 231 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 290

Query: 263 HVRLPSDTSHALYHHGDA-----SARQKPSDKLRFLNKACIITEGE--DEERSGDPWRVC 322
            ++LP++    + ++  A      ++   S++ R L+KA ++ EG+   E    D WR+C
Sbjct: 291 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGDLTPEGPPADKWRLC 350

Query: 323 RVEQVEDLKSIIKVIPIWSTGIL-FSVSFNQNTLPYLQVSTMDRHITPNIEIPAGSFIMF 382
            V++VE++K +I+++PIWS GI+  +    Q T    Q   MDR++ P  EIPAGS  + 
Sbjct: 351 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 410

Query: 383 LFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEIKRRAM 442
             +++G+++  YDR+ VP   +I G  + +  LQ+IG GI  +  S+    +VE  RR  
Sbjct: 411 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 470

Query: 443 AIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVSTSLSG 502
           +I  G   +   +  MS FWL P  +   + +A  +IGQ EFF SQ P+ M S++ SL  
Sbjct: 471 SINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFS 530

Query: 503 LSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLLYFVAC 555
           LS +  S ++ F++TVV   +       W++ N+N    +Y++ LI  +  ++L+YF  C
Sbjct: 531 LSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYC 590

BLAST of Moc08g03300 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.2e-87
Identity = 201/560 (35.89%), Postives = 311/560 (55.54%), Query Frame = 0

Query: 10  EIGAEEPLLGGGPK--KGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTS 69
           +I  EE  L G P   KGG+ T+PFI  NE  E     G  +N   YL  +  +   + +
Sbjct: 8   KISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAA 67

Query: 70  NFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCDK 129
           N L   +    LTP+LGAF+ADS+AGRF TI F +    +GMTLL I++ IP  RP   K
Sbjct: 68  NTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCK 127

Query: 130 SLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILE--SFIGCY 189
               C      QL +LY   +L  +G GGI+   +AFG DQ   +      +  ++   Y
Sbjct: 128 GEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWY 187

Query: 190 YVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFT 249
           Y       L  +TV+V+IQDN+ W LG GIP   M   +++F+    L+  + P+ S FT
Sbjct: 188 YFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFT 247

Query: 250 SLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKL------RFLNKACIITEGE 309
            L QV VA++R R +R+ SD S  LY + +  A      KL       FL+KA I+TE E
Sbjct: 248 RLIQVGVAAFRKRKLRMVSDPS-LLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTE-E 307

Query: 310 DEERSG---DPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSF-NQNTLPYLQVSTMDRH 369
           D  + G   + WR+  V +VE+LKS+I++ PI ++GIL   ++  Q T    Q  TM+RH
Sbjct: 308 DNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRH 367

Query: 370 ITPNIEIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCI 429
           +T + +IPAGS  +F  +++   I+ YDR+ V +A K  G    +  L ++G G  +S I
Sbjct: 368 LTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISII 427

Query: 430 SVASIAVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYS 489
           +      VE+KR+++AI+ G  ++ H +V +S  WL+P Y    +A+A   IG  EFFY 
Sbjct: 428 ATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYD 487

Query: 490 QLPKSMSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWV-SSNINKAHYEYYFL 549
           Q P+SM S +T+L  +++S G+ V+  ++T+V   +    G +W+  +N+N+   EY++ 
Sbjct: 488 QAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYW 547

Query: 550 LICGICFIDLLYFVACCRSY 555
           LI  +  ++L+Y++ C + Y
Sbjct: 548 LITVLQAVNLVYYLWCAKIY 565

BLAST of Moc08g03300 vs. ExPASy TrEMBL
Match: A0A6J1BPF3 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004589 PE=3 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 566/568 (99.65%), Postives = 566/568 (99.65%), Query Frame = 0

Query: 1   MADSNGAAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYG 60
           MADSNGAAEEI AEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYG
Sbjct: 1   MADSNGAAEEIXAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYG 60

Query: 61  MSALQTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQ 120
           MSALQTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQ
Sbjct: 61  MSALQTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQ 120

Query: 121 ARPYCDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILES 180
           ARPYCDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILES
Sbjct: 121 ARPYCDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILES 180

Query: 181 FIGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPS 240
           FIGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPS
Sbjct: 181 FIGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPS 240

Query: 241 SSLFTSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGED 300
           SSLFTSLFQVVVA YRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGED
Sbjct: 241 SSLFTSLFQVVVAXYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGED 300

Query: 301 EERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNI 360
           EERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNI
Sbjct: 301 EERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNI 360

Query: 361 EIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASI 420
           EIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASI
Sbjct: 361 EIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASI 420

Query: 421 AVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKS 480
           AVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKS
Sbjct: 421 AVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKS 480

Query: 481 MSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGIC 540
           MSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGIC
Sbjct: 481 MSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGIC 540

Query: 541 FIDLLYFVACCRSYDGGDEEDETCKLQD 569
           FIDLLYFVACCRSYDGGDEEDETCKLQD
Sbjct: 541 FIDLLYFVACCRSYDGGDEEDETCKLQD 568

BLAST of Moc08g03300 vs. ExPASy TrEMBL
Match: A0A7N2N3T0 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 8.0e-159
Identity = 295/563 (52.40%), Postives = 391/563 (69.45%), Query Frame = 0

Query: 5   NGAAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSAL 64
           N + E+   +EPLL     KGG +TLPFII NE+ E   S GL  N   YL  E+GM   
Sbjct: 3   NPSDEKDMVKEPLLNTYKTKGGFKTLPFIIANEAFEGVASFGLMPNMILYLTREFGMQIA 62

Query: 65  QTSNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPY 124
             +N L+  S      P+LGA+LADSY GR+R I FG+  + LGM LLW+T+   QA+  
Sbjct: 63  SAANVLFIWSAATNFMPILGAYLADSYVGRYRMIGFGSIVSLLGMVLLWLTTLNSQAKIS 122

Query: 125 CDKSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESF 184
           C +S S C+  TAF+L  LYS F LM IG GG+++SSLAFG DQL      +N  +LESF
Sbjct: 123 CSQSSSSCNFSTAFKLLHLYSSFGLMAIGAGGLRSSSLAFGADQLDKRDNIRNAAMLESF 182

Query: 185 IGCYYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSS 244
              YYV     +L  +T IVY+QD + W +GFGIPA LM   ++SF L SP ++K++  S
Sbjct: 183 FSWYYVSKTASALIAVTFIVYVQDKMGWMVGFGIPALLMSLSVLSFFLASPFYIKLKSDS 242

Query: 245 SLFTSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACII----TE 304
           SL T   QV+VASY+NR  RL S  +  +YHH + S    PS+KLRFLNKACII     +
Sbjct: 243 SLLTGFTQVLVASYKNRRTRLSSQATPEMYHHINGSMLLLPSEKLRFLNKACIIKNPLQD 302

Query: 305 GEDEERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHIT 364
              + R+ DPW +C V QVE+LK++I+VIP+WS GI+ SVS +QN+ P LQ ++MDRHIT
Sbjct: 303 LSPDGRATDPWSLCTVNQVEELKALIRVIPLWSAGIMMSVSISQNSFPVLQAASMDRHIT 362

Query: 365 PNIEIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISV 424
           P  EIPAGSF +FL I++ LWI LYDR+++PLASKI GKP RL   +++G G+F SC+S+
Sbjct: 363 PYFEIPAGSFSVFLVITITLWITLYDRVVLPLASKISGKPCRLNEKKRMGIGLFFSCMSM 422

Query: 425 ASIAVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQL 484
           A++AV E  RR +AI++GFS++  AVV MSA WLLP+Y+FA + +A   IGQNEF+YSQL
Sbjct: 423 AAMAVAESVRRELAIEEGFSDDPQAVVSMSAMWLLPYYIFAGLGEAFNAIGQNEFYYSQL 482

Query: 485 PKSMSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLIC 544
           PKSMSS++T+L GL MSA SLVA FI++ VD+VTK  GGESWVSSNINKAHY+YY+ L+ 
Sbjct: 483 PKSMSSIATTLFGLGMSAASLVASFILSTVDNVTKK-GGESWVSSNINKAHYDYYYWLLT 542

Query: 545 GICFIDLLYFVACCRSYDGGDEE 561
           G+C  +L+Y++ C ++Y   + E
Sbjct: 543 GLCSANLMYYLFCSKAYGPSEGE 564

BLAST of Moc08g03300 vs. ExPASy TrEMBL
Match: A0A6A1UVR4 (Protein NRT1/ PTR FAMILY 1.2 OS=Morella rubra OX=262757 GN=CJ030_MR8G014178 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.5e-157
Identity = 296/559 (52.95%), Postives = 383/559 (68.52%), Query Frame = 0

Query: 9   EEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSN 68
           EE   + PLL     KG  RTLPFII NE+ E   S GL  N   YL   YG+     +N
Sbjct: 8   EEDTIKHPLLRN--PKGCFRTLPFIIANEAFERAASFGLLPNMILYLTRVYGLETASAAN 67

Query: 69  FLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITS-AIPQARPYCDK 128
            ++         P++GA+LADSY GR+R I FG   + +GM L W+TS   PQA+  C K
Sbjct: 68  AIFLWQAASNFAPIIGAYLADSYVGRYRMIGFGCIVSLVGMVLFWLTSTTAPQAQVPCSK 127

Query: 129 SLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKH---ILESFIGC 188
           S S C  P+AFQL ++YS F LM IG GGI++ SLAFG DQL    N     +LESF   
Sbjct: 128 SSSSCIFPSAFQLLLVYSSFGLMAIGAGGIRSCSLAFGADQLEKRDNVRDAGMLESFFSW 187

Query: 189 YYVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLF 248
           YYV + V +LF +T IVYIQDN+ WS+GFGIPA  M    +SF L SP +VK++ +SSL 
Sbjct: 188 YYVSSAVSALFAVTFIVYIQDNLGWSVGFGIPAVFMFLSALSFFLASPYYVKLKANSSLL 247

Query: 249 TSLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEG----ED 308
           T   QVVV+SYRNRH+RLPS  +   YH    S    PS+K+RFLNKACII +       
Sbjct: 248 TGFAQVVVSSYRNRHIRLPSQATAEAYHQTKGSMLLMPSEKVRFLNKACIIRDPLQVLNP 307

Query: 309 EERSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNI 368
           E R+ DPW +C V QVE+LK++I++IP+WSTGI+ S + NQ++ P LQ ++M+RHITP  
Sbjct: 308 EGRASDPWSLCTVNQVEELKALIRIIPLWSTGIMISATMNQSSFPVLQATSMNRHITPYF 367

Query: 369 EIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASI 428
           EIPAGSF MFL ISL LWI LYDR+++PLASKI GKPT L    ++  G+ +SC+S+A++
Sbjct: 368 EIPAGSFSMFLIISLTLWIALYDRVVLPLASKICGKPTHLSEKNRMAIGLVLSCVSMAAM 427

Query: 429 AVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKS 488
           A+VE  RR +A ++GFS+   AVV MSA WLLPHYVFA +A+A   IGQNEF+YSQLP++
Sbjct: 428 AIVESIRRKVAAEEGFSDFPQAVVNMSAMWLLPHYVFAGVAEAFNAIGQNEFYYSQLPRT 487

Query: 489 MSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGIC 548
           MSS+++SL G+ MSAG+LVA FIM+ VDD TK  GGESWVSSNINK HY+YYF L+  + 
Sbjct: 488 MSSIASSLLGVGMSAGALVASFIMSTVDDSTKK-GGESWVSSNINKGHYDYYFWLLAFLG 547

Query: 549 FIDLLYFVACCRSYDGGDE 560
            I+++YF+AC + Y   +E
Sbjct: 548 LINVVYFLACSKVYGSCEE 563

BLAST of Moc08g03300 vs. ExPASy TrEMBL
Match: A0A6J1BUQ4 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004912 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 1.0e-153
Identity = 280/558 (50.18%), Postives = 384/558 (68.82%), Query Frame = 0

Query: 7   AAEEIGAEEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQT 66
           A E+   EEPLL     KGG+RTLPFII N ++E   S GL+ +   YL   YGM +   
Sbjct: 7   ADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAA 66

Query: 67  SNFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCD 126
           S  ++  S     TP++ AFLADSY GRF  IA G+  + LGM +LW+T+ IPQARP+CD
Sbjct: 67  STVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCD 126

Query: 127 KSLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILESFIGCYY 186
           +    C++P+  QL +LYS + LM IG G +++SS+AFG DQL   K+   L+++    Y
Sbjct: 127 ELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLL--KSSGTLKNYFSLVY 186

Query: 187 VFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTS 246
           +    G L GM+ IVYIQD++ W++GFG+P A MLF  ++FL  SPL+VK  PSSSL T 
Sbjct: 187 MAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTG 246

Query: 247 LFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGEDE----E 306
           L QVVVASY+ R +++PS  ++ +YHH + S    PSDKLRFLNKACII+  E+E     
Sbjct: 247 LVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEELTSDG 306

Query: 307 RSGDPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNIEI 366
           R+ +PW +C V+QVE LK++I+++P+WSTGIL +V  NQ +   LQ+++MDRH+TP  E+
Sbjct: 307 RASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQLASMDRHLTPTFEV 366

Query: 367 PAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAV 426
           PAGSF + L +SL +WI+LYDRL++PL SKI GK TRL    ++G GI     S+ + A+
Sbjct: 367 PAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVATAI 426

Query: 427 VEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMS 486
           VE  RRAMAI++GFS++  AVV MSAFW LP Y+F  +++    +GQ EFFY +LP++MS
Sbjct: 427 VETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMS 486

Query: 487 SVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFI 546
           SV+TSL GL MS G+L A FIMT+VD+ +K GGGESWVS+NIN  H +YY+ L+  + F 
Sbjct: 487 SVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVFA 546

Query: 547 DLLYFVACCRSYDGGDEE 561
           + LYF+ C +SY    EE
Sbjct: 547 NFLYFLVCSKSYGPSKEE 561

BLAST of Moc08g03300 vs. ExPASy TrEMBL
Match: A0A445KXH0 (Protein NRT1/ PTR FAMILY 1.2 isoform A OS=Glycine soja OX=3848 GN=D0Y65_009131 PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.9e-152
Identity = 277/549 (50.46%), Postives = 381/549 (69.40%), Query Frame = 0

Query: 14  EEPLLG--GGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLY 73
           EEPLL       KGG RTLPFII NE+ E   S GL  N   YL  EYGM     +NFL 
Sbjct: 12  EEPLLSKQNSNAKGGFRTLPFIIANEAFERLASLGLMPNMILYLTREYGMQTAGATNFLL 71

Query: 74  FRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCDKSLSR 133
             S     TP +GA L+DSY GR+  IAFG+  + LGM LLW+T+ IP ++P C++  + 
Sbjct: 72  LWSAASNFTPFVGAVLSDSYVGRYSMIAFGSIASLLGMVLLWLTTLIPLSKPLCNQFTNS 131

Query: 134 C-DSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLA-TNKNKHILESFIGCYYVFT 193
           C +SPT   L +L+S F LM IG GGI++SSLAFG DQL+  +KN  I ES+   YY   
Sbjct: 132 CNNSPTTIHLLILHSSFALMSIGAGGIRSSSLAFGVDQLSKRDKNAGIKESYFSWYYAIV 191

Query: 194 LVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQ 253
            + SL G+TV+VYIQDN+ W++GFGIP  LM    VSF L SP +V +E   ++ + L Q
Sbjct: 192 AMSSLIGLTVVVYIQDNMGWAVGFGIPVILMFVATVSFFLASPFYVMVEVKRNMLSGLAQ 251

Query: 254 VVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEG----EDEERSG 313
           V+VASY+NR ++LP +T + +YH    S    P++KLRFLNKAC+I         E R+ 
Sbjct: 252 VLVASYKNRLLQLPQETENGIYHLEKDSDLLMPTEKLRFLNKACLIRNSLQDLTPEGRAL 311

Query: 314 DPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPNIEIPAG 373
           +PW +C V+QVE+LK++IK++PIWSTGI+  V+ +Q +L  L+ S+MDRHIT N EIP+G
Sbjct: 312 NPWNLCTVDQVEELKALIKIVPIWSTGIMMGVNISQGSLLVLEASSMDRHITSNFEIPSG 371

Query: 374 SFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEI 433
           SF+ F+ +SL LW+++YDR+LVP+ASKI G P  +   QK+G G+   CI++AS+AVVE 
Sbjct: 372 SFVTFMIVSLVLWVIIYDRILVPVASKIKGSPACIGAKQKMGIGLLTGCIAIASLAVVED 431

Query: 434 KRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVS 493
            RR +AI++G+ ++  AVV MSA WLLP  +   +A+A  V+GQNEFF ++LP+SMSS++
Sbjct: 432 IRRKIAIEKGYEDQPQAVVNMSALWLLPRQILNGLAEALGVVGQNEFFLTELPQSMSSLA 491

Query: 494 TSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLL 553
           ++L+GL  S  +LVA FI++VVD+VT  GG ESW+SSNINK HY+YY+ LIC +CF++ +
Sbjct: 492 STLNGLGSSVANLVASFILSVVDNVTGGGGHESWLSSNINKGHYDYYYTLICALCFVNFV 551

Query: 554 YFVACCRSY 555
           YF+ C +SY
Sbjct: 552 YFLYCSKSY 560

BLAST of Moc08g03300 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 491.1 bits (1263), Expect = 1.2e-138
Identity = 262/549 (47.72%), Postives = 362/549 (65.94%), Query Frame = 0

Query: 20  GGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFRSTLGGL 79
           G   KGGI T+PFII NE+ E   S GL  N   YL+ +Y     + +N L+  S     
Sbjct: 18  GKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNF 77

Query: 80  TPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPY-CDKSL--SRCDSPT 139
           TP+LGAFL+DSY GRF TI+  +  + LGM LLW+T+ +PQ +P  CD +   S C S T
Sbjct: 78  TPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSST 137

Query: 140 AFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESFIGCYYVFTLVGS 199
           A QL +LYS F L+ IG GGI+  SLAFG DQL      KN+ +LESF G YY  + V  
Sbjct: 138 ASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAV 197

Query: 200 LFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQVVVA 259
           L   T IVYIQ+++ W +GFG+PA LML   + F+L SPL+V    + SLFT L Q +VA
Sbjct: 198 LIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVA 257

Query: 260 SYRNRHVRLPS-DTSHALYHHGDASARQKPSDKLRFLNKACIITEGEDEERSG----DPW 319
           +Y+ R + LP    S   Y+H   S  + PS KLRFLNKAC+I+  E+E  S     +PW
Sbjct: 258 AYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPW 317

Query: 320 RVCRVEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHIT---PNIEIPAG 379
           R+C  ++VE+LK++IKVIPIWSTGI+ S++ +Q++   LQ ++MDR ++    + ++PAG
Sbjct: 318 RLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAG 377

Query: 380 SFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEI 439
           SF MF  I+L LW++LYDR ++PLASKI G+P RL    ++G G+FMS +++A  A+VE 
Sbjct: 378 SFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVES 437

Query: 440 KRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVS 499
            RR  AI QG++N S+AVV +SA WL+P YV   +A+A   IGQ EFFY++ PKSMSS++
Sbjct: 438 FRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIA 497

Query: 500 TSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLL 555
            SL GL M+  SL+A  ++  V+++T   G ESWVS NINK HY YY+ ++  + FI+++
Sbjct: 498 ASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVI 557

BLAST of Moc08g03300 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 456.1 bits (1172), Expect = 4.2e-128
Identity = 247/544 (45.40%), Postives = 347/544 (63.79%), Query Frame = 0

Query: 24  KGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFRSTLGGLTPVL 83
           KGG+ T+PFII NE  E   S GL  N   YLM +Y +  ++    L+         P++
Sbjct: 24  KGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLV 83

Query: 84  GAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPY-CDKSL-SRCDSPTAFQLF 143
           GAFL+DSY GRF TI   +  + LGM +LW+T+ +PQ +P  C  +  + C S T+ QL 
Sbjct: 84  GAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLA 143

Query: 144 VLYSCFVLMCIGGGGIKASSLAFGGDQLATN---KNKHILESFIGCYYVFTLVGSLFGMT 203
           +LY+ F L+ IG GGI+  SLAFG DQL      KN+ +LESF G YY  + V  L   T
Sbjct: 144 LLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFT 203

Query: 204 VIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQVVVASYRNR 263
           VIVYIQD++ W +GFGIPA LML     F+  SPL+VK + S SLFT L QVV A+Y  R
Sbjct: 204 VIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKR 263

Query: 264 HVRLPS-DTSHALYHHGDASARQKPSDKLRFLNKACIITEGEDEERSGD-----PWRVCR 323
           ++ LP    S   Y+    S  + PSDKLRFLNKAC I+   DE+   D      WR+C 
Sbjct: 264 NLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAIS-NRDEDLGSDGLALNQWRLCT 323

Query: 324 VEQVEDLKSIIKVIPIWSTGILFSVSFNQNTLPYLQVSTMDRHITPN--IEIPAGSFIMF 383
            +QVE LK+++KVIP+WSTGI+ S++ +QN+   LQ  +MDR ++ N   +IPAGSF MF
Sbjct: 324 TDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMF 383

Query: 384 LFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEIKRRAM 443
             I+L  W++LYDR ++PLASKI G+P R+    ++G G+F+S +++A  A VE  RR  
Sbjct: 384 TIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKT 443

Query: 444 AIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVSTSLSG 503
           AI QG +N++++ V +SA WL+P YV   +A+A   IGQ EFFY++ PKSMSS++ SL G
Sbjct: 444 AISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFG 503

Query: 504 LSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLLYFVAC 555
           L M+  +++A  I+  V + +K  G  SW+  NINK HY+YY+ ++  + F++++Y+V C
Sbjct: 504 LGMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVC 563

BLAST of Moc08g03300 vs. TAIR 10
Match: AT5G11570.1 (Major facilitator superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 7.1e-91
Identity = 204/547 (37.29%), Postives = 296/547 (54.11%), Query Frame = 0

Query: 14  EEPLLGGGPKKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFR 73
           E  L+G    K GI T+PFI+ ++++E     GL  N   +L  EYGM   + +N L+  
Sbjct: 4   EALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILFLW 63

Query: 74  STLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCDKSLSRCD 133
           S      P++GAF+ADSY GRF  I FG++ +  GM LLW+T+ I   RP CDK  + C 
Sbjct: 64  SAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNVCQ 123

Query: 134 SPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHI----LESFIGCYYVFT 193
             T  +  +LYS F L  IG GG+++S LAF  DQL  N+   +    LE+    YY   
Sbjct: 124 PTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYFSV 183

Query: 194 LVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQ 253
           +V      +++V++Q    W +GFG+  A M   +  F   SP +V+             
Sbjct: 184 MVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYVRF------------ 243

Query: 254 VVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKLRFLNKACIITEGEDEERSGDPWR 313
                                         QKP+                    S +PW+
Sbjct: 244 ------------------------------QKPT------------------RNSRNPWK 303

Query: 314 VCRVEQVEDLKSIIKVIPIWSTGILFS-VSFNQNTLPYLQVSTMDRH-ITPNIEIPAGSF 373
           +CRV+QVEDLKS+I VIPIWSTGI+ S V+  Q +   LQ  TMDRH      EIP GS+
Sbjct: 304 LCRVQQVEDLKSLINVIPIWSTGIILSLVTACQVSFIVLQAKTMDRHTFIQGFEIPPGSY 363

Query: 374 IMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEIKR 433
            +FL IS  L++ LYD ++VPL S  L +P RL  + ++  G  +S + ++++A  E  R
Sbjct: 364 GIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISALAATEYAR 423

Query: 434 RAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVSTS 493
           R  A          +  K+SA WLLP+ +   IA+A   I QNEFFYS+LPK+MSSV+T+
Sbjct: 424 RKTA-------RDESGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKTMSSVATT 476

Query: 494 LSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLLYF 553
           LS L+M+A SL++ +I+T+V DVT  G   SW++ NI++ H +YY+ L+ G+  +++LYF
Sbjct: 484 LSSLNMAAASLISSWIITIV-DVTTYG---SWITENIDEGHLDYYYWLLVGLSLLNVLYF 476

Query: 554 VACCRSY 555
           V C +SY
Sbjct: 544 VWCKKSY 476

BLAST of Moc08g03300 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 327.4 bits (838), Expect = 2.3e-89
Identity = 186/544 (34.19%), Postives = 300/544 (55.15%), Query Frame = 0

Query: 23  KKGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTSNFLYFRSTLGGLTPV 82
           K GG R + FI+GNE++E   S GL +NF  YL   + +  +  +N +   S    LTP+
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 83  LGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCDKSLS--RCDSPTAFQL 142
           +GA+++D+Y GRF+TIAF +  T LG+  + +T++ PQ  P    S     C  P   Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 143 FVLYSCFVLMCIGGGGIKASSLAFGGDQL--ATNKNKHILESFIGCYYVFTLVGSLFGMT 202
            VL      + +G GGI+  S+ FG DQ    T +    + SF   YY+   V  +   T
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 230

Query: 203 VIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFTSLFQVVVASYRNR 262
           V+VYIQD +SW +GF IP  LM   +V F      +V ++P  S+F+ + QV+VA+ + R
Sbjct: 231 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 290

Query: 263 HVRLPSDTSHALYHHGDA-----SARQKPSDKLRFLNKACIITEGE--DEERSGDPWRVC 322
            ++LP++    + ++  A      ++   S++ R L+KA ++ EG+   E    D WR+C
Sbjct: 291 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEGDLTPEGPPADKWRLC 350

Query: 323 RVEQVEDLKSIIKVIPIWSTGIL-FSVSFNQNTLPYLQVSTMDRHITPNIEIPAGSFIMF 382
            V++VE++K +I+++PIWS GI+  +    Q T    Q   MDR++ P  EIPAGS  + 
Sbjct: 351 SVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVI 410

Query: 383 LFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCISVASIAVVEIKRRAM 442
             +++G+++  YDR+ VP   +I G  + +  LQ+IG GI  +  S+    +VE  RR  
Sbjct: 411 SLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIR 470

Query: 443 AIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYSQLPKSMSSVSTSLSG 502
           +I  G   +   +  MS FWL P  +   + +A  +IGQ EFF SQ P+ M S++ SL  
Sbjct: 471 SINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFS 530

Query: 503 LSMSAGSLVAGFIMTVVDDVTKAGGGESWVSSNINKAHYEYYFLLICGICFIDLLYFVAC 555
           LS +  S ++ F++TVV   +       W++ N+N    +Y++ LI  +  ++L+YF  C
Sbjct: 531 LSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYC 590

BLAST of Moc08g03300 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 8.6e-89
Identity = 201/560 (35.89%), Postives = 311/560 (55.54%), Query Frame = 0

Query: 10  EIGAEEPLLGGGPK--KGGIRTLPFIIGNESIESCVSSGLSSNFTPYLMGEYGMSALQTS 69
           +I  EE  L G P   KGG+ T+PFI  NE  E     G  +N   YL  +  +   + +
Sbjct: 8   KISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAA 67

Query: 70  NFLYFRSTLGGLTPVLGAFLADSYAGRFRTIAFGAAFTSLGMTLLWITSAIPQARPYCDK 129
           N L   +    LTP+LGAF+ADS+AGRF TI F +    +GMTLL I++ IP  RP   K
Sbjct: 68  NTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCK 127

Query: 130 SLSRCDSPTAFQLFVLYSCFVLMCIGGGGIKASSLAFGGDQLATNKNKHILE--SFIGCY 189
               C      QL +LY   +L  +G GGI+   +AFG DQ   +      +  ++   Y
Sbjct: 128 GEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWY 187

Query: 190 YVFTLVGSLFGMTVIVYIQDNISWSLGFGIPAALMLFFLVSFLLVSPLFVKIEPSSSLFT 249
           Y       L  +TV+V+IQDN+ W LG GIP   M   +++F+    L+  + P+ S FT
Sbjct: 188 YFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFT 247

Query: 250 SLFQVVVASYRNRHVRLPSDTSHALYHHGDASARQKPSDKL------RFLNKACIITEGE 309
            L QV VA++R R +R+ SD S  LY + +  A      KL       FL+KA I+TE E
Sbjct: 248 RLIQVGVAAFRKRKLRMVSDPS-LLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTE-E 307

Query: 310 DEERSG---DPWRVCRVEQVEDLKSIIKVIPIWSTGILFSVSF-NQNTLPYLQVSTMDRH 369
           D  + G   + WR+  V +VE+LKS+I++ PI ++GIL   ++  Q T    Q  TM+RH
Sbjct: 308 DNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRH 367

Query: 370 ITPNIEIPAGSFIMFLFISLGLWILLYDRLLVPLASKILGKPTRLRPLQKIGFGIFMSCI 429
           +T + +IPAGS  +F  +++   I+ YDR+ V +A K  G    +  L ++G G  +S I
Sbjct: 368 LTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISII 427

Query: 430 SVASIAVVEIKRRAMAIQQGFSNESHAVVKMSAFWLLPHYVFAAIADASCVIGQNEFFYS 489
           +      VE+KR+++AI+ G  ++ H +V +S  WL+P Y    +A+A   IG  EFFY 
Sbjct: 428 ATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYD 487

Query: 490 QLPKSMSSVSTSLSGLSMSAGSLVAGFIMTVVDDVTKAGGGESWV-SSNINKAHYEYYFL 549
           Q P+SM S +T+L  +++S G+ V+  ++T+V   +    G +W+  +N+N+   EY++ 
Sbjct: 488 QAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYW 547

Query: 550 LICGICFIDLLYFVACCRSY 555
           LI  +  ++L+Y++ C + Y
Sbjct: 548 LITVLQAVNLVYYLWCAKIY 565