Moc08g03250 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc08g03250 vs. NCBI nr
Match: XP_038885421.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Benincasa hispida])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 472/545 (86.61%), Postives = 508/545 (93.21%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNRLKRNVFPSPNFTVRSIASPQRHRPLP-VAPPPEIDPSGHR 60
           MAS+A GS I  PFN KTN        S NFT+RSIASP R RP P V P PEID +G  
Sbjct: 2   MASMAIGSSIISPFNFKTNHHPSKPTLS-NFTIRSIASPIR-RPSPIVVPLPEIDTTG-P 61

Query: 61  NNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKL 120
            NHVAWTS+RQ++WEGELSV+GQLP WLSGTYLRNGPG+WNIGDYNFRHLFDGYATIVKL
Sbjct: 62  PNHVAWTSVRQDRWEGELSVRGQLPRWLSGTYLRNGPGLWNIGDYNFRHLFDGYATIVKL 121

Query: 121 QFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASLTD 180
            FDDGR+IAGHRQIES+AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+LFSGASLTD
Sbjct: 122 HFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGASLTD 181

Query: 181 NSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDSEF 240
           N+NTGV+KLGDGRVVCLTETQKGSIMID  +LET+GKFE+SDSLGGLIHSAHPIVTD+EF
Sbjct: 182 NANTGVIKLGDGRVVCLTETQKGSIMIDPTTLETVGKFEYSDSLGGLIHSAHPIVTDTEF 241

Query: 241 LTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRY 300
           LTLLPDL NPGY VVRM+PG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPLRY
Sbjct: 242 LTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRY 301

Query: 301 CAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERDEE 360
           CAQNLLRAEPTPLYKFEWRPESKAF+HVMCKASG IV SVEVPL+ITFHFINAYEERDE+
Sbjct: 302 CAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGNIVASVEVPLFITFHFINAYEERDEK 361

Query: 361 GRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAA 420
           GRITAVIADCCEHNANPVILDRLRL+NLRR +LK ALPDARVGRFRIP+DGSG G LEAA
Sbjct: 362 GRITAVIADCCEHNANPVILDRLRLHNLRR-SLKYALPDARVGRFRIPLDGSGYGKLEAA 421

Query: 421 LEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGA 480
           L+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+EDGA
Sbjct: 422 LDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYEDGA 481

Query: 481 IPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHG 540
           IPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHG
Sbjct: 482 IPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHG 541

Query: 541 CWVPK 544
           CWVPK
Sbjct: 542 CWVPK 542

BLAST of Moc08g03250 vs. NCBI nr
Match: XP_023546621.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 967.6 bits (2500), Expect = 4.5e-278
Identity = 465/547 (85.01%), Postives = 503/547 (91.96%), Query Frame = 0

Query: 1   MASIAAGSCI--FPFNS--KTNRLKRNVFPSPNFTVRSIASPQRHRPLPVAPPPEIDPSG 60
           MAS+AAGSCI   P+N     +  KR V    NFT+RSIASP R  P  V P P+ID S 
Sbjct: 1   MASMAAGSCINFKPYNPCLPLHPPKRTV---SNFTIRSIASPIRPSPPVVVPLPDIDTS- 60

Query: 61  HRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIV 120
               HVAWTSIRQ++WEG+LSVQGQLP WL+GTYLRNGPGIWNIG+YNFRHLFDGYATIV
Sbjct: 61  TPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGIWNIGNYNFRHLFDGYATIV 120

Query: 121 KLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASL 180
           KL F+DGR+IAGHRQIES AYMAA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYMAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 181 TDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDS 240
           TDN+NTGVVKLGDGRVVCLTETQKGSI+I+  +LET+GKFE+SDSLGGLIHSAHPIVTDS
Sbjct: 181 TDNANTGVVKLGDGRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDS 240

Query: 241 EFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPL 300
           EFLTLLPDL NPGY VVRM+PG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERD 360
           RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 361 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLE 420
           E+GRITAVIADCCEHNANP+ILDRLRL+NLRRF  K ALPDARVGRFRIP+DGSG+G LE
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFTPKHALPDARVGRFRIPLDGSGHGKLE 420

Query: 421 AALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHED 480
           AAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E 
Sbjct: 421 AALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYES 480

Query: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540
           GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL
Sbjct: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540

Query: 541 HGCWVPK 544
           HGCW+PK
Sbjct: 541 HGCWIPK 543

BLAST of Moc08g03250 vs. NCBI nr
Match: KAG6598778.1 (Carotenoid cleavage dioxygenase 8-like B, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 965.3 bits (2494), Expect = 2.2e-277
Identity = 463/547 (84.64%), Postives = 503/547 (91.96%), Query Frame = 0

Query: 1   MASIAAGSCIF--PFNS--KTNRLKRNVFPSPNFTVRSIASPQRHRPLPVAPPPEIDPSG 60
           MAS+AAGSCI   P+N     +  KR V    NFT+RSIASP R  P  V P P+ID S 
Sbjct: 1   MASMAAGSCISFKPYNPCLPFHPPKRTV---SNFTIRSIASPIRPSPPVVVPLPDIDTS- 60

Query: 61  HRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIV 120
               HVAWTSIRQ++WEG+LSVQGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATIV
Sbjct: 61  IPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIV 120

Query: 121 KLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASL 180
           KL F+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 181 TDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDS 240
           TDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFE+SDSLGGLIHSAHPIVTDS
Sbjct: 181 TDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDS 240

Query: 241 EFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPL 300
           EFLTLLPDL NPGY VVRM+PG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERD 360
           RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 361 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLE 420
           E+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G LE
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLE 420

Query: 421 AALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHED 480
           AAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E 
Sbjct: 421 AALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYES 480

Query: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540
           GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL
Sbjct: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540

Query: 541 HGCWVPK 544
           HGCW+PK
Sbjct: 541 HGCWIPK 543

BLAST of Moc08g03250 vs. NCBI nr
Match: XP_023538517.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.1 bits (2491), Expect = 4.9e-277
Identity = 462/547 (84.46%), Postives = 506/547 (92.50%), Query Frame = 0

Query: 1   MASIAAGSCIFPFNSKT-NRLKRNVFPSPNFTVRSIASPQRHRPLPVAPPPEIDPSGHRN 60
           MAS+AAGSC+  FN K+ +  KR VF   NFT+RSIA+P R  P    P PEID   H  
Sbjct: 1   MASMAAGSCMSSFNFKSIHPPKRTVF---NFTIRSIATPIR-PPSVGVPLPEIDTPAH-P 60

Query: 61  NHVAWTSIRQEKWEGELSVQGQLPYWL---SGTYLRNGPGIWNIGDYNFRHLFDGYATIV 120
           +HVAWTS++QE+WEGELSVQG+LP WL   +GTYLRNGPGIW+IGDY+FRHLFDGYATIV
Sbjct: 61  SHVAWTSVQQERWEGELSVQGELPRWLVRFNGTYLRNGPGIWHIGDYDFRHLFDGYATIV 120

Query: 121 KLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASL 180
           KLQFDDGR+IAGHRQIES+AY AA++NQK+C+REFSEVPKANNFLAYVGELASLFSGASL
Sbjct: 121 KLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKANNFLAYVGELASLFSGASL 180

Query: 181 TDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDS 240
           TDN+NTGVVKLGDGRV+CLTETQKGSIMI+  +LET+GKFE+SDSLGGLIHSAHPIVTDS
Sbjct: 181 TDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTDS 240

Query: 241 EFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPL 300
           EFLTLLPDL NPGY VVRM+PG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERD 360
           RYCAQNLLRAEPTPLYKFEWRPESKA++HVMCKASGKIV SVEVPLYITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEERD 360

Query: 361 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLE 420
           E+GRITAVIADCCEHNANP+ILDRLRL+NLRRF   DALPDAR+GRFRIP+DGSG G LE
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFTPNDALPDARLGRFRIPLDGSGYGELE 420

Query: 421 AALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHED 480
           AAL+PEEHGRG+DMCSFNPA+LGKKY YAYACGA+RPCNFPNTLTKIDLV+K AKNWHE 
Sbjct: 421 AALDPEEHGRGLDMCSFNPAYLGKKYGYAYACGAQRPCNFPNTLTKIDLVKKTAKNWHET 480

Query: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540
           GAIPSEPFFVARPGATEEDDGVVISMVSG++GEGYALLLDGSTFEEIARAKFPYGLPYGL
Sbjct: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYGL 540

Query: 541 HGCWVPK 544
           HGCW+PK
Sbjct: 541 HGCWIPK 542

BLAST of Moc08g03250 vs. NCBI nr
Match: XP_022997653.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 964.1 bits (2491), Expect = 4.9e-277
Identity = 463/548 (84.49%), Postives = 503/548 (91.79%), Query Frame = 0

Query: 1   MASIAAGSCIFPFNSKTNRL-----KRNVFPSPNFTVRSIASPQRHRPLPVAPPPEIDPS 60
           MAS+AAGSCI  F +    L     KR+V    NFT+RSIASP R  P  V P P ID S
Sbjct: 1   MASMAAGSCI-SFKAYNPCLPLHPPKRSV---SNFTIRSIASPIRPSPPVVVPFPNIDTS 60

Query: 61  GHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATI 120
                HVAWTSIRQ++WEG+LS+QGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATI
Sbjct: 61  -TPPTHVAWTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATI 120

Query: 121 VKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGAS 180
           VKLQF+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG S
Sbjct: 121 VKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVS 180

Query: 181 LTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTD 240
           LTDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFE+SDSLGGLIHSAHPIVTD
Sbjct: 181 LTDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTD 240

Query: 241 SEFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMP 300
           SEFLTLLPDL NPGY VVRM+PG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 241 SEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEER
Sbjct: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEER 360

Query: 361 DEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTL 420
           DE+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G L
Sbjct: 361 DEKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKL 420

Query: 421 EAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHE 480
           EAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E
Sbjct: 421 EAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYE 480

Query: 481 DGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540
            GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG
Sbjct: 481 SGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540

Query: 541 LHGCWVPK 544
           LHGCW+PK
Sbjct: 541 LHGCWIPK 543

BLAST of Moc08g03250 vs. ExPASy Swiss-Prot
Match: Q8LIY8 (Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8B PE=1 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 1.8e-237
Identity = 377/512 (73.63%), Postives = 435/512 (84.96%), Query Frame = 0

Query: 43  PLPVAPPP--EIDPSGHRNNH---------VAWTSIRQEKWEGELSVQGQLPYWLSGTYL 102
           P PV  PP   +D    R            VAW S+RQE+WEG L V G+LP WL GTYL
Sbjct: 53  PAPVVAPPARPVDAPRRRGGRGGGGGGGELVAWKSVRQERWEGALEVDGELPLWLDGTYL 112

Query: 103 RNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREF 162
           RNGPG+WN+GDY FRHLFDGYAT+V++ F  GR +  HRQIESEAY AA  + KVC+REF
Sbjct: 113 RNGPGLWNLGDYGFRHLFDGYATLVRVSFRGGRAVGAHRQIESEAYKAARAHGKVCYREF 172

Query: 163 SEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLE 222
           SEVPK +NFL+YVG+LA+LFSG+SLTDNSNTGVV LGDGRV+CLTET KGSI +D D+L+
Sbjct: 173 SEVPKPDNFLSYVGQLATLFSGSSLTDNSNTGVVMLGDGRVLCLTETIKGSIQVDPDTLD 232

Query: 223 TLGKFEFSDSLGGLIHSAHPIVTDSEFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCR 282
           T+GKF+++D LGGLIHSAHPIVTD+EF TL+PDL  PGY V RMD G+NER+ +GRV+CR
Sbjct: 233 TVGKFQYTDKLGGLIHSAHPIVTDTEFWTLIPDLIRPGYVVARMDAGSNERQFVGRVDCR 292

Query: 283 GGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKAS 342
           GGPAPGWVHSFPVTE+YVVVPEMPLRYCA+NLLRAEPTPLYKFEW  ES ++MHVMCKAS
Sbjct: 293 GGPAPGWVHSFPVTEHYVVVPEMPLRYCAKNLLRAEPTPLYKFEWHLESGSYMHVMCKAS 352

Query: 343 GKIVTSVEVPLYITFHFINAYEERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFAL 402
           GKIV SVEVP ++TFHFINAYEE DEEGR+TA+IADCCEHNAN  ILD+LRL+NLR  + 
Sbjct: 353 GKIVASVEVPPFVTFHFINAYEETDEEGRVTAIIADCCEHNANTAILDKLRLHNLRSSSG 412

Query: 403 KDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAK 462
           +D LPDARVGRFRIP+DGS  G LE AL+PEEHGRGMDMCS NPA +G++YRYAYACGA+
Sbjct: 413 QDVLPDARVGRFRIPLDGSQFGELETALDPEEHGRGMDMCSINPAHVGREYRYAYACGAR 472

Query: 463 RPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGY 522
           RPCNFPNTLTK+DLVE+ AKNWHE+G++PSEPFFV RPGATEEDDGV ISMVS K+G GY
Sbjct: 473 RPCNFPNTLTKVDLVERTAKNWHEEGSVPSEPFFVPRPGATEEDDGVAISMVSAKDGSGY 532

Query: 523 ALLLDGSTFEEIARAKFPYGLPYGLHGCWVPK 544
           AL+LDG TFEE+ARAKFPYGLPYGLH CWVP+
Sbjct: 533 ALVLDGKTFEEVARAKFPYGLPYGLHCCWVPR 564

BLAST of Moc08g03250 vs. ExPASy Swiss-Prot
Match: Q8VY26 (Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD8 PE=1 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 1.4e-213
Identity = 344/518 (66.41%), Postives = 420/518 (81.08%), Query Frame = 0

Query: 33  RSIASPQRHRPLPVAPPPEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLR 92
           R++ +     PLP     E    G R  HVAWTS++QE WEGEL+VQG++P WL+GTYLR
Sbjct: 56  RAVINSAAPSPLPEKEKVE----GERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLR 115

Query: 93  NGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFS 152
           NGPG+WNIGD++FRHLFDGY+T+VKLQFD GR+ A HR +ES+AY AA ++ ++C+REFS
Sbjct: 116 NGPGLWNIGDHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFS 175

Query: 153 EVPKA----NNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSD 212
           E PK+     N  + +GE+  LFSG SLTDN+NTGV+KLGDGRV+CLTETQKGSI++D +
Sbjct: 176 ETPKSVIINKNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHE 235

Query: 213 SLETLGKFEFSDSLGG-LIHSAHPIVTDSEFLTLLPDLFNPGYQVVRMDPGTNERKVIGR 272
           +LET+GKFE+ D L   +I SAHPIVT++E  TL+PDL  PGY+VVRM+ G+N+R+V+GR
Sbjct: 236 TLETIGKFEYDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGR 295

Query: 273 VNCRGGP-APGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHV 332
           V CR G   PGWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HV
Sbjct: 296 VRCRSGSWGPGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHV 355

Query: 333 MCKASGKIVTSVEVPLYITFHFINAYEE-RDEEGRITAVIADCCEHNANPVILDRLRLNN 392
           M K +G++V SVEVP Y+TFHFINAYEE ++ +G+ T +IADCCEHNA+  ILD LRL+ 
Sbjct: 356 MSKLTGEVVASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDT 415

Query: 393 LRRFALKDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYA 452
           LR     D LPDAR+GRFRIP+DGS  G LE A+E E+HGR MDMCS NP +LG+KYRY 
Sbjct: 416 LRSSHGHDVLPDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYV 475

Query: 453 YACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMVSG 512
           YACGA+RPCNFPN L+K+D+VEKK KNWHE G IPSEPFFV RPGAT EDDGVVIS+VS 
Sbjct: 476 YACGAQRPCNFPNALSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSE 535

Query: 513 KNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWVPK 544
           +NG  +A+LLDGS+FEEIARAKFPYGLPYGLHGCW+PK
Sbjct: 536 ENGGSFAILLDGSSFEEIARAKFPYGLPYGLHGCWIPK 569

BLAST of Moc08g03250 vs. ExPASy Swiss-Prot
Match: Q93VD5 (Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8A PE=2 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 8.1e-198
Identity = 318/486 (65.43%), Postives = 390/486 (80.25%), Query Frame = 0

Query: 63  AWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDD 122
           A TS+RQE+WEG+L ++G LP WL+GTY+RNGPG+W++G++ F HLFDGYAT+V++ F  
Sbjct: 68  ASTSVRQERWEGDLPIEGCLPPWLNGTYIRNGPGMWDVGEHAFHHLFDGYATLVRVSFRG 127

Query: 123 G---RVIAGHRQIESEAYMAAVRNQKVCFREFSEVP-KANNFLAYVGELASLFSGASLTD 182
           G   R    HRQIESEAY AAV   +   REFS  P  A + L   G+L  L +GA+LTD
Sbjct: 128 GGGARATGAHRQIESEAYRAAVARGRPVLREFSHCPAPAKSLLHRFGDLVGLVTGAALTD 187

Query: 183 NSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDSEF 242
           N N+ V+ LGDGRV+CLTET K S++ID D+LET+G+F ++D LGG++ SAHPIVTD+EF
Sbjct: 188 NPNSAVLPLGDGRVMCLTETTKSSVLIDPDTLETVGRFRYTDRLGGMVQSAHPIVTDTEF 247

Query: 243 LTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRY 302
           LTLLPDL  PG+ VVRM+ G+NERKVIGR++CRGGP+PGW+HSF VTE Y VVPEMPLRY
Sbjct: 248 LTLLPDLVRPGHLVVRMEAGSNERKVIGRMDCRGGPSPGWLHSFAVTEKYAVVPEMPLRY 307

Query: 303 CAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERDEE 362
            + +LL +E  P Y F+W P S ++MHVMCK++GK V SVEVP ++  HFINAYE   EE
Sbjct: 308 SSASLLASELAPFYAFDWVPASGSYMHVMCKSTGKTVASVEVPPFMAIHFINAYE---EE 367

Query: 363 GRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAA 422
           G   AV+ DCCEH  +P I++ L L+ LR    KD LP+ARVGRFRIP+DGS  G LE A
Sbjct: 368 GDEAAVVVDCCEHYGDPAIIETLVLSRLRLLRGKDVLPNARVGRFRIPLDGSPFGELETA 427

Query: 423 LEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGA 482
           L+PEEHGRGMDMCS NPA LG+KY+YAYACGA+RPCNFPNTLTKIDLVEKKAK+WHE+G+
Sbjct: 428 LDPEEHGRGMDMCSINPARLGRKYQYAYACGARRPCNFPNTLTKIDLVEKKAKSWHEEGS 487

Query: 483 IPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHG 542
           +PSEPFFVARPGAT+EDDGVVIS+VS  +GEGYAL+LD +TFEEIAR +FPYGLPYG HG
Sbjct: 488 VPSEPFFVARPGATDEDDGVVISIVSSDDGEGYALVLDATTFEEIARVRFPYGLPYGFHG 547

Query: 543 CWVPKT 545
           CW+P T
Sbjct: 548 CWIPAT 550

BLAST of Moc08g03250 vs. ExPASy Swiss-Prot
Match: Q8HXG8 (Beta,beta-carotene 9',10'-oxygenase OS=Macaca fascicularis OX=9541 GN=BCO2 PE=2 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.9e-50
Identity = 148/521 (28.41%), Postives = 235/521 (45.11%), Query Frame = 0

Query: 78  VQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAY 137
           V G  P WL+G+ LR GPG +  G   + H FDG A + + +   G V    + ++S+ Y
Sbjct: 49  VWGHFPKWLNGSLLRIGPGKFEFGKDKYNHWFDGMALLHQFRMAKGTVTYRSKFLQSDTY 108

Query: 138 MAAVRNQKVCFREFSEV----PKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVV 197
            A     ++   EF  +    P  N F  ++         A++TDN+N   V+      +
Sbjct: 109 KANSAKNRIVMSEFGTLATPDPCKNVFERFMSRFELPGKAAAMTDNTNVNYVRYKGDYYL 168

Query: 198 CLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDSEFLTLLPDLFNP---GY 257
           C TET   +  +D ++LE   K ++S  +     +AHP          + + F P    Y
Sbjct: 169 C-TETNFMN-KVDIETLEKTEKVDWSKFIAVNGATAHPHYDPDGTAYNMGNSFGPFGFSY 228

Query: 258 QVVRMDP-------GTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNL 317
           +V+R+ P        T+  +VI  +       P + HSF +T NY++  E PL+    NL
Sbjct: 229 KVIRVPPEKVDLEETTHGAQVICSIAPTEKGKPSYYHSFGMTRNYIIFIEQPLK---MNL 288

Query: 318 LRAEPTPLY------KFEWRPESKAFMHVMCKASGKIVTS-VEVPLYITFHFINAYEERD 377
            +   + +          W P+     HV+ K +G+++        ++ FH INA+E++ 
Sbjct: 289 WKIATSKIRGKAFSDGISWEPQCNTRFHVVDKHTGQLLPGRYYSKPFVAFHHINAFEDQG 348

Query: 378 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFA-----LKDALPDARVGRFRIPMDGSG 437
                  +I  CC+ N    IL+  +L NLR+       + ++   +   RF +P++ S 
Sbjct: 349 -----CVIIDLCCQDNGR--ILEVYQLQNLRKAGEELDQVYNSAGRSFPRRFVLPLNVSL 408

Query: 438 NGTLEAALEP--------EEHGRGMDMCS--------------------FNPAFLGKKYR 497
           N      L P         +   G   CS                    +   F GKKYR
Sbjct: 409 NAPEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYGQFSGKKYR 468

Query: 498 YAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMV 543
           + Y CG +      ++L K+D+V K  K W EDG  PSEP FV  PG  EED GV++S+V
Sbjct: 469 FFYGCGFRHLVG--DSLIKVDVVNKTLKVWREDGFYPSEPVFVPVPGTNEEDGGVILSVV 528

BLAST of Moc08g03250 vs. ExPASy Swiss-Prot
Match: Q9BYV7 (Beta,beta-carotene 9',10'-oxygenase OS=Homo sapiens OX=9606 GN=BCO2 PE=1 SV=5)

HSP 1 Score: 198.4 bits (503), Expect = 2.1e-49
Identity = 146/521 (28.02%), Postives = 233/521 (44.72%), Query Frame = 0

Query: 78  VQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAY 137
           V G  P WL+G+ LR GPG +  G   + H FDG A + + +   G V    + ++S+ Y
Sbjct: 72  VWGHFPKWLNGSLLRIGPGKFEFGKDKYNHWFDGMALLHQFRMAKGTVTYRSKFLQSDTY 131

Query: 138 MAAVRNQKVCFREFSEV----PKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVV 197
            A     ++   EF  +    P  N F  ++         A++TDN+N   V+      +
Sbjct: 132 KANSAKNRIVISEFGTLALPDPCKNVFERFMSRFELPGKAAAMTDNTNVNYVRYKGDYYL 191

Query: 198 CLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDSEFLTLLPDLFNP---GY 257
           C TET   +  +D ++LE   K ++S  +     +AHP          + + F P    Y
Sbjct: 192 C-TETNFMN-KVDIETLEKTEKVDWSKFIAVNGATAHPHYDLDGTAYNMGNSFGPYGFSY 251

Query: 258 QVVRMDPGTNER-------KVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNL 317
           +V+R+ P   +        +VI  +       P + HSF +T NY++  E PL+    NL
Sbjct: 252 KVIRVPPEKVDLGETIHGVQVICSIASTEKGKPSYYHSFGMTRNYIIFIEQPLK---MNL 311

Query: 318 LRAEPTPLY------KFEWRPESKAFMHVMCKASGKIVTS-VEVPLYITFHFINAYEERD 377
            +   + +          W P+     HV+ K +G+++        ++TFH INA+E++ 
Sbjct: 312 WKIATSKIRGKAFSDGISWEPQCNTRFHVVEKRTGQLLPGRYYSKPFVTFHQINAFEDQG 371

Query: 378 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFA-----LKDALPDARVGRFRIPMDGSG 437
                  +I  CC+ N     L+  +L NLR+       + ++   +   RF +P++ S 
Sbjct: 372 -----CVIIDLCCQDNGR--TLEVYQLQNLRKAGEGLDQVHNSAAKSFPRRFVLPLNVSL 431

Query: 438 NGTLEAALEP--------EEHGRGMDMCS--------------------FNPAFLGKKYR 497
           N      L P         +   G   CS                    +   F GKKY 
Sbjct: 432 NAPEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYDRFSGKKYH 491

Query: 498 YAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMV 543
           + Y CG +      ++L K+D+V K  K W EDG  PSEP FV  PG  EED GV++S+V
Sbjct: 492 FFYGCGFRHLVG--DSLIKVDVVNKTLKVWREDGFYPSEPVFVPAPGTNEEDGGVILSVV 551

BLAST of Moc08g03250 vs. ExPASy TrEMBL
Match: A0A6J1KEJ8 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492518 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.4e-277
Identity = 463/548 (84.49%), Postives = 503/548 (91.79%), Query Frame = 0

Query: 1   MASIAAGSCIFPFNSKTNRL-----KRNVFPSPNFTVRSIASPQRHRPLPVAPPPEIDPS 60
           MAS+AAGSCI  F +    L     KR+V    NFT+RSIASP R  P  V P P ID S
Sbjct: 1   MASMAAGSCI-SFKAYNPCLPLHPPKRSV---SNFTIRSIASPIRPSPPVVVPFPNIDTS 60

Query: 61  GHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATI 120
                HVAWTSIRQ++WEG+LS+QGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATI
Sbjct: 61  -TPPTHVAWTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATI 120

Query: 121 VKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGAS 180
           VKLQF+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG S
Sbjct: 121 VKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVS 180

Query: 181 LTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTD 240
           LTDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFE+SDSLGGLIHSAHPIVTD
Sbjct: 181 LTDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTD 240

Query: 241 SEFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMP 300
           SEFLTLLPDL NPGY VVRM+PG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 241 SEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEER
Sbjct: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEER 360

Query: 361 DEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTL 420
           DE+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G L
Sbjct: 361 DEKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKL 420

Query: 421 EAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHE 480
           EAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E
Sbjct: 421 EAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYE 480

Query: 481 DGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540
            GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG
Sbjct: 481 SGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540

Query: 541 LHGCWVPK 544
           LHGCW+PK
Sbjct: 541 LHGCWIPK 543

BLAST of Moc08g03250 vs. ExPASy TrEMBL
Match: A0A6J1HGQ6 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462801 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.5e-276
Identity = 461/547 (84.28%), Postives = 502/547 (91.77%), Query Frame = 0

Query: 1   MASIAAGSCIF--PFNS--KTNRLKRNVFPSPNFTVRSIASPQRHRPLPVAPPPEIDPSG 60
           MAS+AAGSCI   P+N     +  KR V    +FT+RSIASP R  P  V P P+ID S 
Sbjct: 1   MASMAAGSCISFKPYNPCLPLHPPKRTV---SSFTIRSIASPIRPSPPVVVPLPDIDTS- 60

Query: 61  HRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIV 120
               HVAWTSIRQ++WEG+LSVQGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATIV
Sbjct: 61  IPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIV 120

Query: 121 KLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASL 180
           KL F+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 181 TDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVTDS 240
           TDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFE+SDSLGGLIHSAHPIVTD 
Sbjct: 181 TDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDL 240

Query: 241 EFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPL 300
           EFLTLLPDL NPGY VVRM+PG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERD 360
           RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 361 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLE 420
           E+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G LE
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLE 420

Query: 421 AALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHED 480
           AAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E 
Sbjct: 421 AALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYES 480

Query: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540
           GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL
Sbjct: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540

Query: 541 HGCWVPK 544
           HGCW+PK
Sbjct: 541 HGCWIPK 543

BLAST of Moc08g03250 vs. ExPASy TrEMBL
Match: A0A0A0LS17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373590 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 7.7e-276
Identity = 464/550 (84.36%), Postives = 501/550 (91.09%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNRLKRNVFPS-----PNFTVRSIASPQRHRPLPVAPP-PEID 60
           MAS+A GSCI  PFN KT      + PS      NFT+RSIASP  H P PV  P PEID
Sbjct: 2   MASMAMGSCIISPFNFKTKHPILPIQPSSKPTLSNFTIRSIASPV-HPPSPVVVPLPEID 61

Query: 61  PSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYA 120
            S   NNHVAWTSIRQ++WEGELS+QG LP WLSGTYLRNGPG+WNIGDYNFRHLFDGYA
Sbjct: 62  TS---NNHVAWTSIRQDRWEGELSIQGHLPSWLSGTYLRNGPGLWNIGDYNFRHLFDGYA 121

Query: 121 TIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSG 180
           TIVKL FD+GR+IAGHRQIES AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+LFSG
Sbjct: 122 TIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 181

Query: 181 ASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIV 240
           ASLTDN+NTGVVKLGDGRVVCLTETQKGSIMID D+LET+G+FE+SDSLGGLIHSAHPIV
Sbjct: 182 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGQFEYSDSLGGLIHSAHPIV 241

Query: 241 TDSEFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPE 300
           TDSEFLTLLPDL NPGY VVRM+P +NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPE
Sbjct: 242 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 301

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYE 360
           MPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYE
Sbjct: 302 MPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 361

Query: 361 ERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNG 420
           ERDE+GRITAVIADCCEHNA+P ILDRLRL+NLR  +LK  LPDARVGRFRIP+DGSG G
Sbjct: 362 ERDEKGRITAVIADCCEHNADPAILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYG 421

Query: 421 TLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNW 480
            LEAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGA+RPCNFPNTLTKIDLV+K AKNW
Sbjct: 422 KLEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKVAKNW 481

Query: 481 HEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP 540
           +E+G IPSEPFFVARPGATEEDDGVVISMVS +NGEGYALLLDGSTFEEIARAKFPYGLP
Sbjct: 482 YEEGTIPSEPFFVARPGATEEDDGVVISMVSAQNGEGYALLLDGSTFEEIARAKFPYGLP 541

Query: 541 YGLHGCWVPK 544
           YGLHGCWVPK
Sbjct: 542 YGLHGCWVPK 546

BLAST of Moc08g03250 vs. ExPASy TrEMBL
Match: A0A5A7VHT5 (Carotenoid cleavage dioxygenase 8-like protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003670 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 462/553 (83.54%), Postives = 499/553 (90.24%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNRLKRNVFP---------SPNFTVRSIASPQRHRPLPVAPPP 60
           MAS+A GSCI  PFN KT   K  + P           NFT+RSIASP R     V P P
Sbjct: 2   MASMAIGSCIISPFNFKT---KHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLP 61

Query: 61  EIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFD 120
           EID +    NHVAWTSIRQ++WEGELSVQG LP WLSGTYLRNGPG+WNIGDY+FRHLFD
Sbjct: 62  EID-TFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFD 121

Query: 121 GYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASL 180
           GYA IVKL FD+GR+IAGHRQIES AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+L
Sbjct: 122 GYAMIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANL 181

Query: 181 FSGASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAH 240
           FSGASLTDN+NTGVVKLGDGRVVCLTETQKGSIMID D+LET+GKFE+SDSLGGLIHSAH
Sbjct: 182 FSGASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAH 241

Query: 241 PIVTDSEFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVV 300
           PIVTDSEFLTLLPDL NPGY VVRM+P +NERKVIGRVNC+GGPAPGWVHSFPVTENYVV
Sbjct: 242 PIVTDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVV 301

Query: 301 VPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFIN 360
           VPEMPLRYCAQNLL+AEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFIN
Sbjct: 302 VPEMPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFIN 361

Query: 361 AYEERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGS 420
           AYEERDE+GRITAVIADCCEHNANPVILDRLRL+NLR  +LK  LPDARVGRFRIP+DGS
Sbjct: 362 AYEERDEKGRITAVIADCCEHNANPVILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGS 421

Query: 421 GNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKA 480
           G G LEAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGA+RPCNFPNTLTKIDLV+K A
Sbjct: 422 GYGELEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKMA 481

Query: 481 KNWHEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPY 540
           KNW+E+G +PSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPY
Sbjct: 482 KNWYEEGTVPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPY 541

Query: 541 GLPYGLHGCWVPK 544
           GLPYGLHGCWVPK
Sbjct: 542 GLPYGLHGCWVPK 549

BLAST of Moc08g03250 vs. ExPASy TrEMBL
Match: A0A1S3BBP5 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=CCD8 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 462/553 (83.54%), Postives = 499/553 (90.24%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNRLKRNVFP---------SPNFTVRSIASPQRHRPLPVAPPP 60
           MAS+A GSCI  PFN KT   K  + P           NFT+RSIASP R     V P P
Sbjct: 2   MASMAIGSCIISPFNFKT---KHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLP 61

Query: 61  EIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFD 120
           EID +    NHVAWTSIRQ++WEGELSVQG LP WLSGTYLRNGPG+WNIGDY+FRHLFD
Sbjct: 62  EID-TFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFD 121

Query: 121 GYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASL 180
           GYA IVKL FD+GR+IAGHRQIES AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+L
Sbjct: 122 GYAMIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANL 181

Query: 181 FSGASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEFSDSLGGLIHSAH 240
           FSGASLTDN+NTGVVKLGDGRVVCLTETQKGSIMID D+LET+GKFE+SDSLGGLIHSAH
Sbjct: 182 FSGASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAH 241

Query: 241 PIVTDSEFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVV 300
           PIVTDSEFLTLLPDL NPGY VVRM+P +NERKVIGRVNC+GGPAPGWVHSFPVTENYVV
Sbjct: 242 PIVTDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVV 301

Query: 301 VPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFIN 360
           VPEMPLRYCAQNLL+AEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFIN
Sbjct: 302 VPEMPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFIN 361

Query: 361 AYEERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGS 420
           AYEERDE+GRITAVIADCCEHNANPVILDRLRL+NLR  +LK  LPDARVGRFRIP+DGS
Sbjct: 362 AYEERDEKGRITAVIADCCEHNANPVILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGS 421

Query: 421 GNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKA 480
           G G LEAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGA+RPCNFPNTLTKIDLV+K A
Sbjct: 422 GYGELEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKMA 481

Query: 481 KNWHEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPY 540
           KNW+E+G +PSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPY
Sbjct: 482 KNWYEEGTVPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPY 541

Query: 541 GLPYGLHGCWVPK 544
           GLPYGLHGCWVPK
Sbjct: 542 GLPYGLHGCWVPK 549

BLAST of Moc08g03250 vs. TAIR 10
Match: AT4G32810.1 (carotenoid cleavage dioxygenase 8 )

HSP 1 Score: 743.8 bits (1919), Expect = 9.7e-215
Identity = 344/518 (66.41%), Postives = 420/518 (81.08%), Query Frame = 0

Query: 33  RSIASPQRHRPLPVAPPPEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLR 92
           R++ +     PLP     E    G R  HVAWTS++QE WEGEL+VQG++P WL+GTYLR
Sbjct: 56  RAVINSAAPSPLPEKEKVE----GERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLR 115

Query: 93  NGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFS 152
           NGPG+WNIGD++FRHLFDGY+T+VKLQFD GR+ A HR +ES+AY AA ++ ++C+REFS
Sbjct: 116 NGPGLWNIGDHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFS 175

Query: 153 EVPKA----NNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSD 212
           E PK+     N  + +GE+  LFSG SLTDN+NTGV+KLGDGRV+CLTETQKGSI++D +
Sbjct: 176 ETPKSVIINKNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHE 235

Query: 213 SLETLGKFEFSDSLGG-LIHSAHPIVTDSEFLTLLPDLFNPGYQVVRMDPGTNERKVIGR 272
           +LET+GKFE+ D L   +I SAHPIVT++E  TL+PDL  PGY+VVRM+ G+N+R+V+GR
Sbjct: 236 TLETIGKFEYDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGR 295

Query: 273 VNCRGGP-APGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHV 332
           V CR G   PGWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HV
Sbjct: 296 VRCRSGSWGPGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHV 355

Query: 333 MCKASGKIVTSVEVPLYITFHFINAYEE-RDEEGRITAVIADCCEHNANPVILDRLRLNN 392
           M K +G++V SVEVP Y+TFHFINAYEE ++ +G+ T +IADCCEHNA+  ILD LRL+ 
Sbjct: 356 MSKLTGEVVASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDT 415

Query: 393 LRRFALKDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYA 452
           LR     D LPDAR+GRFRIP+DGS  G LE A+E E+HGR MDMCS NP +LG+KYRY 
Sbjct: 416 LRSSHGHDVLPDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYV 475

Query: 453 YACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMVSG 512
           YACGA+RPCNFPN L+K+D+VEKK KNWHE G IPSEPFFV RPGAT EDDGVVIS+VS 
Sbjct: 476 YACGAQRPCNFPNALSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSE 535

Query: 513 KNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWVPK 544
           +NG  +A+LLDGS+FEEIARAKFPYGLPYGLHGCW+PK
Sbjct: 536 ENGGSFAILLDGSSFEEIARAKFPYGLPYGLHGCWIPK 569

BLAST of Moc08g03250 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-27
Identity = 135/527 (25.62%), Postives = 223/527 (42.31%), Query Frame = 0

Query: 34  SIASP-QRHRPLPVAPPPEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLR 93
           SI  P +++RPLP    P +  SG+      +  + +   +  L V GQ+P  L G Y+R
Sbjct: 80  SIVIPMEQNRPLPKPTDPAVQLSGN------FAPVNECPVQNGLEVVGQIPSCLKGVYIR 139

Query: 94  NGPGIWNIGDYNFRHLFDGYATI--VKLQFDDG-----RVIAGHRQIESEAYMAAVRNQK 153
           NG            HLFDG   I  V + FD+      R    +R ++  A   +V  + 
Sbjct: 140 NGANPM-FPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKP 199

Query: 154 VC-FREFSEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQ-KGSI 213
           +      S + +   F A  G    L  G      +N GVV   +GR++ ++E      +
Sbjct: 200 IGELHGHSGLARLALFTARAG--IGLVDGTRGMGVANAGVV-FFNGRLLAMSEDDLPYQV 259

Query: 214 MIDSD-SLETLGKFEFSDSLGGLIHSAHPIV--TDSEFLTLLPD-LFNPGYQVVRMDP-G 273
            ID    LET+G+F F D +   +  AHP V  T  +  TL  + L  P  + ++ +  G
Sbjct: 260 KIDGQGDLETIGRFGFDDQIDSSV-IAHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCG 319

Query: 274 TNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRP 333
              R V   +     P P  +H F +TEN+VV+P+  + +    ++R     +Y      
Sbjct: 320 KKTRDVEITL-----PEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYV----K 379

Query: 334 ESKAFMHVMCK--ASGKIVTSVEVPLYITFHFINAYEERDEEGRITAVIADCCEHNANPV 393
           E  A   V+ K   +G  +  V+VP    FH  NA+EER EEG    V+   C    + +
Sbjct: 380 EKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTI 439

Query: 394 ILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPA 453
                       F+         +   R+ M      T E+  +    G  ++    N +
Sbjct: 440 ------------FSESGEPTRVELSEIRLNM-----RTKESNRKVIVTGVNLEAGHINRS 499

Query: 454 FLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNW-HEDGAIPSEPFFVARPGATEED 513
           ++G+K ++ Y   A  P    + + K+D+       + +       EP FV   G  EED
Sbjct: 500 YVGRKSQFVYIAIAD-PWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE-GEGEED 559

Query: 514 DGVVISMV-SGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWV 542
            G V+  V   +  E   +++D +  +++A  + P  +PYG HG +V
Sbjct: 560 KGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFV 567

BLAST of Moc08g03250 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 107.5 bits (267), Expect = 3.5e-23
Identity = 120/523 (22.94%), Postives = 214/523 (40.92%), Query Frame = 0

Query: 35  IASPQRHRPLPVAPPPEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNG 94
           + S ++  PLP    P +  +G+      +  + ++     L V G+LP  + G Y+RNG
Sbjct: 104 LVSHEKLHPLPKTADPSVQIAGN------FAPVNEQPVRRNLPVVGKLPDSIKGVYVRNG 163

Query: 95  PGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFRE---- 154
               +       H FDG   +  ++F+ G      R  ++  ++   +  +  F +    
Sbjct: 164 ANPLH-EPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGE 223

Query: 155 -FSEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQ---KGSIMID 214
                  A   L Y    A +   A  T  +N G+V   +GR++ ++E     +  I  +
Sbjct: 224 LHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYF-NGRLLAMSEDDLPYQVQITPN 283

Query: 215 SDSLETLGKFEFSDSLGGLIHSAHPIV--TDSEFLTLLPDLFNPGY-QVVRMDP-GTNER 274
            D L+T+G+F+F   L   +  AHP V     E   L  D+ +  Y +  R  P GT   
Sbjct: 284 GD-LKTVGRFDFDGQLESTM-IAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSP 343

Query: 275 KVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKA 334
            V  +++      P  +H F +TEN+VVVP+  + +    ++R     +Y         A
Sbjct: 344 DVEIQLD-----QPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYD----KNKVA 403

Query: 335 FMHVMCK--ASGKIVTSVEVPLYITFHFINAYEERDEEGRITAVIADCCEHNANPVILDR 394
              ++ K       +  ++ P    FH  NA+EE + +     V+   C    + +    
Sbjct: 404 RFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETD---EVVVIGSCMTPPDSIF--- 463

Query: 395 LRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAFLGK 454
               N     LK  L + R+         +G  T    +  E+    ++    N   LG+
Sbjct: 464 ----NESDENLKSVLSEIRLNL------KTGESTRRPIISNEDQQVNLEAGMVNRNMLGR 523

Query: 455 KYRYAYACGAKRPCNFPNTLTKIDLVEKKA-KNWHEDGAIPSEPFFVARPGATEEDDGVV 514
           K ++AY   A+ P    +   K+DL   +  K+ + D     EP F+   G  EED+G +
Sbjct: 524 KTKFAYLALAE-PWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGG-EEDEGYI 583

Query: 515 ISMV-SGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWV 542
           +  V   K  +    +++  + E  A  K P  +PYG HG ++
Sbjct: 584 LCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFI 589

BLAST of Moc08g03250 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 91.7 bits (226), Expect = 2.0e-18
Identity = 123/556 (22.12%), Postives = 215/556 (38.67%), Query Frame = 0

Query: 14  NSKTNRLKRNVFPSPNFTVRSIASPQRHR-PLPVAPPPEIDPSGHRNNHVAWTSIRQEKW 73
           N K N ++R      +    ++ S +R R P P    P +  +G+      +  + ++  
Sbjct: 142 NPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGN------FFPVPEKPV 201

Query: 74  EGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQI 133
              L V G +P  + G Y+RNG    +    +  HLFDG   +  ++FD+G V    R  
Sbjct: 202 VHNLPVTGTVPECIQGVYVRNGANPLH-KPVSGHHLFDGDGMVHAVRFDNGSVSYACRFT 261

Query: 134 ESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKLGD--- 193
           E+   +     ++ C R     PKA      +GEL      A L   +  G+  L D   
Sbjct: 262 ETNRLV----QERECGRPV--FPKA------IGELHGHLGIAKLMLFNTRGLFGLVDPTG 321

Query: 194 -------------GRVVCLTETQ--KGSIMIDSDSLETLGKFEFSDSLGGLIHSAHPIVT 253
                        G ++ ++E        +  +  LET G+++F   L   +  AHP + 
Sbjct: 322 GLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTM-IAHPKID 381

Query: 254 DS--EFLTLLPDLFNPGYQVVRMDPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVP 313
               E   L  D+ +  Y          E+     +       P  +H F +TEN+VV+P
Sbjct: 382 PETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPL---DQPTMIHDFAITENFVVIP 441

Query: 314 EMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTS---VEVPLYITFHFI 373
           +  + +    ++R     +Y      E K     +   + K  +S   +EVP    FH  
Sbjct: 442 DQQVVFRLPEMIRGGSPVVY-----DEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLW 501

Query: 374 NAYEERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDG 433
           N++EE + +     V+   C    + +        N     L+  L + R+         
Sbjct: 502 NSWEEPETD---EVVVIGSCMTPPDSIF-------NEHDETLQSVLSEIRLNL------K 561

Query: 434 SGNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKK 493
           +G  T    +  + +   ++    N   LG+K RYAY      P    +   K+DL   +
Sbjct: 562 TGESTRRPVISEQVN---LEAGMVNRNLLGRKTRYAY-LALTEPWPKVSGFAKVDLSTGE 621

Query: 494 AKNW-HEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYAL-LLDGSTFEEIARAK 544
            + + + +G    EP F+   G  EED G ++  V  +      L L++    +  A   
Sbjct: 622 IRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVT 648