Moc08g02800 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g02800
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionEukaryotic aspartyl protease family protein
Locationchr8: 1966773 .. 1970000 (+)
RNA-Seq ExpressionMoc08g02800
SyntenyMoc08g02800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTCAAAATTTATTTCCTCTTTGTCCTCGCGATTCTAATTCTGGGTTTGAAATCCAACGCTTCCGATCCCTGCCCCTCAAAAGCCGACGGCTCCGATGATATCTCCGTCATCCCCATCTACGGCAAATGCTCGCCGTTCACGGCGCCGAAAACCGAGTCGTGGGTGAACACGGTGATGAACATGGCGGCGAAAGACGCGGGCCGAGTGAAGTACCTGTGGAGCCTCGGCGCCCAGAAGAGCGTCGCGGCGCCGATCGCCTCAGGGCAGCAGGTGCTGAATATTGGGAATTACGTGGTCCGAGTTCAACTCGGGACTCCGGGTCAAGTAATGTACATGGTTCTTGACACCAGCAACGACGCCGCATGGGTGCCGTGCTCCGGCTGCGCCGGCTGCTCCGCCGTCACTTTTTCCTCAAAAACTTCCTCCTCGTTTGCCACTTTGGATTGCTCCAAGCCAGAATGCAGCCAGGTTTCTTAAATCATCACATTTTCTCGAAGAATTGCATTTTAATTTGGTCTTGAATTTATATTTTTGTAACATTAAACAAAACTAAATGATTACCTAGGTAAGTAATTTCGTTTAACAAATTAACGTACTAATTTTAAAAAGGAAAATACCTCACTCCTTAATTAGGGGTGAATGAATTTGGTGAGAAATGCAAAAATAAATATTTTTTTAAATAGAATGAATTGTCAAAAATTTTCAACAGTAGTAAAAACAATATTTGGTTAGCCAAATGTTATTCTTTTGAAGATGTATAATCTCCTGTTACACAGACATCTACTAAAATCATTATAGACTAAATGGTATTGAGTGAATAAATATTAAATGACAAGATGATAATAACGACAAAATATAGTTATTTGGCCTTTTTTTTTTGAATTTAGGGAAAAAATTAAACGTTTTAATAGTTGAATAACTCAGAACATTTTGCTCTTTTTTTTTTGGCTAAAAGATGACGTTGCATAATGTTAAATCTACAAATTTAAAGAGAAATTTAAGTTTACACAGTGTAAATTCAAAATTTATTTTAAGAAATGTCAGTTTTTCATTATTTTCAAATATAATATAAATGTCTCCTTTTTGGTACATTTTACATTACACTTGAATAGGTTGTATCATGCTATTTTCTTTTAACTTGAAAAGGCCTTAAAATCATTTTACACCAAAAGAGATGCATAATAATATTCAATTTTCTTCTAATGAAAGATAGCTATATAGATCAATTAATTTCATTTTAGCCTTGAAACGTTTTCCCCCAACTTCCTCCAGAAAAATGTTTCAAATTTAAAAATACCCTTTCTTGCAAATAGATCAGATTTCAATTTACATTATGAGAAATTTATTTATATTTAAAATTCACATTCTTAACAGGTCAGGGGTCTCTCTTGTCCGACCACCGGCTCCGCCGAGTGCCTTTTCAACCAAACTTACGGCGGCGACTCCTCTTTTTCCGCCACCCTCGTTCAAGACTCTCTCCATTTAGGCGGCGACGTAATCCCAAATTTCTCCTTCGGCTGCCTCAGCTCCGCCTCCGGCAGCTCCATTCCGCCGCAAGGCCTAATGGGCCTCGGCCGCGGGCCCCTCTCTCTAATCTCCCAATCCGGTTCACTCTACTCCGGTCTATTCTCCTACTGCCTTCCCAGTTTCAAATCCTACTACTTCTCCGGTTCACTCAAACTCGGCCCGGTCGGCCAGCCAAAGGCAATCCGAACCACCCCGCTTCTGAAGAACCCCCACCGGCCATCGCTGTACTACGTCAACCTGACAGGAATCAGCGTCGGCCGGGTCCTCGTCCCGGTTCAGCCCGAGCATCTCGCATTCGACCCGAACACTGGAGCTGGAACCATAATCGACTCGGGAACCGTAATAACCCGGTTCGTCTCACCGGTCTACGCGGCGGTTCGGGACGAGTTTAGGAAGCAAGTGGGTGGTTCGTTCTCGCCGCTGGGAGCGTTCGACACGTGTTTCGGGACGAGTAACGAAGTTTCGGCGCCGAGTATTACGTTCCATTTCAGCGGCTTGGATTTGAAACTGCCGATGGAGAATAGTTTGATTCACAGCAGCGCCGGGGCGCTGGCTTGCCTGGCCATGGCGGCGGCGCCGAATAATGTGAACTCGGTGCTGAATGTGATTGCCAACTTGCAGCAGCAGAATCATCGGATTTTGTTCGATGTTGCGAATTCTAAATTGGGGATTGCTCGTGAGCTCTGTAATTAAACTGAATCCATAGCCGACGTGTCGTTTCGGGATTATGAATTAATAATTTGGGAAGTGTATGAATATTATTAGGATTGATTTGTGAGTTTTGCTTTTTAGGGGATGGATTCCGCTCTTGGATCCAATTATGTTTGTGTATGGTTAATGCATTTCTGAAGATAATGGTAATAGCATCGTTTTAATGGGCTTGGCCCATTGCATTTTGGGCCTAAGGCCTACAAACTGAAAACTTCTTTTTTATGTTTTTATTTTGAGATAACTTTTTCGGTTTTAGAAACTTATTTATGCCTAAAATTTTCTAATTTTACACTTTCATTCGTGCACGTAGAATTTTATTTTAATTCTGATTTTAACCTTTATGTATTGTATTTACATTTATGGTTATTTATTTTTTTTATTTTTTTAAATATAAAAGATTTAATTCCCTTAATTCACTCCCATATTTGTGCTCAATCTTCAACGACAATTCAAAATCACATAAACATCTTTTGAAGTCTCAATAAACATATATCCGCATATTTAAAAAAATATTCAAAATCATAGTATATATCCACATATATACATTCAAAATCACTATAGTATATATCCGTATATCTCCATATATAAAAGTTGAGTTATAAGATATATCCACTTATATATCCGCATATATACATTCAAAATAACTATAGTATATATCCGCATATCTGCATATATACAAGTTGAGTTATAAGATATATCATAATCAAATCACTATACTATATATGCTCATATATACAATATTTCACACGAAATCAGATTGGAATGCAAACCTTTCAATCTGGAGATGAACTGTTATGGCGACTAAGAAAAATGGAGGCAACCGAAAATCAAGGCAGGCCGACGTCGGCCGAAGACAAAGGCTTTCAGAAACAACTGGAGAATAAGAATCGACAGAAATCTCCTTTCGAAAGCGGTCGCGAGATGAAATGCTCTTGCGACAAAGAAAAGTGGAGCCGACCGAAGAGGAAGACAGACAGCGACTGA

mRNA sequence

ATGGATCTCAAAATTTATTTCCTCTTTGTCCTCGCGATTCTAATTCTGGGTTTGAAATCCAACGCTTCCGATCCCTGCCCCTCAAAAGCCGACGGCTCCGATGATATCTCCGTCATCCCCATCTACGGCAAATGCTCGCCGTTCACGGCGCCGAAAACCGAGTCGTGGGTGAACACGGTGATGAACATGGCGGCGAAAGACGCGGGCCGAGTGAAGTACCTGTGGAGCCTCGGCGCCCAGAAGAGCGTCGCGGCGCCGATCGCCTCAGGGCAGCAGGTGCTGAATATTGGGAATTACGTGGTCCGAGTTCAACTCGGGACTCCGGGTCAAGTAATGTACATGGTTCTTGACACCAGCAACGACGCCGCATGGGTGCCGTGCTCCGGCTGCGCCGGCTGCTCCGCCGTCACTTTTTCCTCAAAAACTTCCTCCTCGTTTGCCACTTTGGATTGCTCCAAGCCAGAATGCAGCCAGGTCAGGGGTCTCTCTTGTCCGACCACCGGCTCCGCCGAGTGCCTTTTCAACCAAACTTACGGCGGCGACTCCTCTTTTTCCGCCACCCTCGTTCAAGACTCTCTCCATTTAGGCGGCGACGTAATCCCAAATTTCTCCTTCGGCTGCCTCAGCTCCGCCTCCGGCAGCTCCATTCCGCCGCAAGGCCTAATGGGCCTCGGCCGCGGGCCCCTCTCTCTAATCTCCCAATCCGGTTCACTCTACTCCGGTCTATTCTCCTACTGCCTTCCCAGTTTCAAATCCTACTACTTCTCCGGTTCACTCAAACTCGGCCCGGTCGGCCAGCCAAAGGCAATCCGAACCACCCCGCTTCTGAAGAACCCCCACCGGCCATCGCTGTACTACGTCAACCTGACAGGAATCAGCGTCGGCCGGGTCCTCGTCCCGGTTCAGCCCGAGCATCTCGCATTCGACCCGAACACTGGAGCTGGAACCATAATCGACTCGGGAACCGTAATAACCCGGTTCGTCTCACCGGTCTACGCGGCGGTTCGGGACGAGTTTAGGAAGCAAGTGGGTGGTTCGTTCTCGCCGCTGGGAGCGTTCGACACGTGTTTCGGGACGAGTAACGAAGTTTCGGCGCCGAGTATTACGTTCCATTTCAGCGGCTTGGATTTGAAACTGCCGATGGAGAATAGTTTGATTCACAGCAGCGCCGGGGCGCTGGCTTGCCTGGCCATGGCGGCGGCGCCGAATAATGTGAACTCGGTGCTGAATGTGATTGCCAACTTGCAGCAGCAGAATCATCGGATTTTGTTCGATGTTGCGAATTCTAAATTGGGGATTGCTCGTGAGCTCTGGGATGGATTCCGCTCTTGGATCCAATTATGTTTGTGTATGGTTAATGCATTTCTGAAGATAATGATTGGAATGCAAACCTTTCAATCTGGAGATGAACTGTTATGGCGACTAAGAAAAATGGAGGCAACCGAAAATCAAGGCAGGCCGACGTCGGCCGAAGACAAAGGCTTTCAGAAACAACTGGAGAATAAGAATCGACAGAAATCTCCTTTCGAAAGCGGTCGCGAGATGAAATGCTCTTGCGACAAAGAAAAGTGGAGCCGACCGAAGAGGAAGACAGACAGCGACTGA

Coding sequence (CDS)

ATGGATCTCAAAATTTATTTCCTCTTTGTCCTCGCGATTCTAATTCTGGGTTTGAAATCCAACGCTTCCGATCCCTGCCCCTCAAAAGCCGACGGCTCCGATGATATCTCCGTCATCCCCATCTACGGCAAATGCTCGCCGTTCACGGCGCCGAAAACCGAGTCGTGGGTGAACACGGTGATGAACATGGCGGCGAAAGACGCGGGCCGAGTGAAGTACCTGTGGAGCCTCGGCGCCCAGAAGAGCGTCGCGGCGCCGATCGCCTCAGGGCAGCAGGTGCTGAATATTGGGAATTACGTGGTCCGAGTTCAACTCGGGACTCCGGGTCAAGTAATGTACATGGTTCTTGACACCAGCAACGACGCCGCATGGGTGCCGTGCTCCGGCTGCGCCGGCTGCTCCGCCGTCACTTTTTCCTCAAAAACTTCCTCCTCGTTTGCCACTTTGGATTGCTCCAAGCCAGAATGCAGCCAGGTCAGGGGTCTCTCTTGTCCGACCACCGGCTCCGCCGAGTGCCTTTTCAACCAAACTTACGGCGGCGACTCCTCTTTTTCCGCCACCCTCGTTCAAGACTCTCTCCATTTAGGCGGCGACGTAATCCCAAATTTCTCCTTCGGCTGCCTCAGCTCCGCCTCCGGCAGCTCCATTCCGCCGCAAGGCCTAATGGGCCTCGGCCGCGGGCCCCTCTCTCTAATCTCCCAATCCGGTTCACTCTACTCCGGTCTATTCTCCTACTGCCTTCCCAGTTTCAAATCCTACTACTTCTCCGGTTCACTCAAACTCGGCCCGGTCGGCCAGCCAAAGGCAATCCGAACCACCCCGCTTCTGAAGAACCCCCACCGGCCATCGCTGTACTACGTCAACCTGACAGGAATCAGCGTCGGCCGGGTCCTCGTCCCGGTTCAGCCCGAGCATCTCGCATTCGACCCGAACACTGGAGCTGGAACCATAATCGACTCGGGAACCGTAATAACCCGGTTCGTCTCACCGGTCTACGCGGCGGTTCGGGACGAGTTTAGGAAGCAAGTGGGTGGTTCGTTCTCGCCGCTGGGAGCGTTCGACACGTGTTTCGGGACGAGTAACGAAGTTTCGGCGCCGAGTATTACGTTCCATTTCAGCGGCTTGGATTTGAAACTGCCGATGGAGAATAGTTTGATTCACAGCAGCGCCGGGGCGCTGGCTTGCCTGGCCATGGCGGCGGCGCCGAATAATGTGAACTCGGTGCTGAATGTGATTGCCAACTTGCAGCAGCAGAATCATCGGATTTTGTTCGATGTTGCGAATTCTAAATTGGGGATTGCTCGTGAGCTCTGGGATGGATTCCGCTCTTGGATCCAATTATGTTTGTGTATGGTTAATGCATTTCTGAAGATAATGATTGGAATGCAAACCTTTCAATCTGGAGATGAACTGTTATGGCGACTAAGAAAAATGGAGGCAACCGAAAATCAAGGCAGGCCGACGTCGGCCGAAGACAAAGGCTTTCAGAAACAACTGGAGAATAAGAATCGACAGAAATCTCCTTTCGAAAGCGGTCGCGAGATGAAATGCTCTTGCGACAAAGAAAAGTGGAGCCGACCGAAGAGGAAGACAGACAGCGACTGA

Protein sequence

MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTVMNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSNDAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYSGLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPVQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTSNEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNHRILFDVANSKLGIARELWDGFRSWIQLCLCMVNAFLKIMIGMQTFQSGDELLWRLRKMEATENQGRPTSAEDKGFQKQLENKNRQKSPFESGREMKCSCDKEKWSRPKRKTDSD
Homology
BLAST of Moc08g02800 vs. NCBI nr
Match: XP_022132317.1 (aspartyl protease AED3 [Momordica charantia])

HSP 1 Score: 868.2 bits (2242), Expect = 3.6e-248
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0

Query: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60
           MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV
Sbjct: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60

Query: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120
           MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN
Sbjct: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120

Query: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180
           DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG
Sbjct: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180

Query: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240
           DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS
Sbjct: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240

Query: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300
           GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP
Sbjct: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300

Query: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360
           VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS
Sbjct: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360

Query: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420
           NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH
Sbjct: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420

Query: 421 RILFDVANSKLGIAREL 438
           RILFDVANSKLGIAREL
Sbjct: 421 RILFDVANSKLGIAREL 437

BLAST of Moc08g02800 vs. NCBI nr
Match: XP_038886172.1 (aspartyl protease AED3 [Benincasa hispida])

HSP 1 Score: 767.7 bits (1981), Expect = 6.6e-218
Identity = 385/437 (88.10%), Postives = 406/437 (92.91%), Query Frame = 0

Query: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60
           M LKI+F F LA+L+  L +NASD C S +DG  D+SVIPIYGKCSPFTAPK+ESWVNTV
Sbjct: 1   MALKIFFFFFLALLV--LNTNASDLCASGSDG--DLSVIPIYGKCSPFTAPKSESWVNTV 60

Query: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120
           ++MA+KD  R+KYL +L AQK+VAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN
Sbjct: 61  IDMASKDPARIKYLSTLAAQKTVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120

Query: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180
           DAAW PCSGC GCSA TFSS  SS+FATLDCSKP+CSQ RGLSCPTTG+A+CLFNQTYGG
Sbjct: 121 DAAWAPCSGCTGCSATTFSSNYSSTFATLDCSKPQCSQARGLSCPTTGNADCLFNQTYGG 180

Query: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240
           DSSFSATLVQDSLHLG DVIPNFSFGC+SSASGSSIPPQGLMGLGRGPLSLISQSGSLYS
Sbjct: 181 DSSFSATLVQDSLHLGTDVIPNFSFGCISSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240

Query: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300
           GLFSYCLPSFKSYYFSGSLKLGP GQPK IRTTPLL NPHRPSLYYVNLTGISVGRVLVP
Sbjct: 241 GLFSYCLPSFKSYYFSGSLKLGPAGQPKLIRTTPLLHNPHRPSLYYVNLTGISVGRVLVP 300

Query: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360
           + PEHLAFDPNTGAGTIIDSGTVITRFVSPVY AVRDEFRKQVGGSFSPLGAFDTCF TS
Sbjct: 301 ISPEHLAFDPNTGAGTIIDSGTVITRFVSPVYTAVRDEFRKQVGGSFSPLGAFDTCFATS 360

Query: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420
           NEVSAP ITFH SGLDLKLPMENSLIHSSAG+LACLAMAAAPNNVNSVLNVIANLQQQNH
Sbjct: 361 NEVSAPGITFHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNH 420

Query: 421 RILFDVANSKLGIAREL 438
           RILFD+ NSKLGIAREL
Sbjct: 421 RILFDITNSKLGIAREL 433

BLAST of Moc08g02800 vs. NCBI nr
Match: XP_022961622.1 (aspartyl protease AED3 [Cucurbita moschata])

HSP 1 Score: 755.0 bits (1948), Expect = 4.5e-214
Identity = 376/437 (86.04%), Postives = 402/437 (91.99%), Query Frame = 0

Query: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60
           M +K  F F LA  +L L SNASD C + +DGS D+SVIPIYGKCSPFTAPK+ESWVNTV
Sbjct: 1   MAVKFVFFFFLA--LLALNSNASDLCAAGSDGSGDLSVIPIYGKCSPFTAPKSESWVNTV 60

Query: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120
           ++MA+KD  R+KYL SL AQK+VAAPIASGQ  LNIGNYVVRVQLGTPGQ MYMVLDTS+
Sbjct: 61  IDMASKDPARIKYLSSLAAQKTVAAPIASGQHALNIGNYVVRVQLGTPGQAMYMVLDTSS 120

Query: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180
           DAAW PCSGC+GCSA TF +K SS+FATLDCSKP+CSQ RGLSCPTTGS +CLFNQTYGG
Sbjct: 121 DAAWAPCSGCSGCSATTFLAKNSSTFATLDCSKPQCSQARGLSCPTTGSVDCLFNQTYGG 180

Query: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240
           DSSFSATLVQD+LHLG DVIPNFSFGC+SSASGSSIPPQGL+GLGRGPLSLISQS SLYS
Sbjct: 181 DSSFSATLVQDTLHLGSDVIPNFSFGCISSASGSSIPPQGLLGLGRGPLSLISQSTSLYS 240

Query: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300
           GLFSYCLPSFKSYYFSGSLKLGPVGQPK+IRTTPLLKNPHRPSLYYVNLTGISVGRVLVP
Sbjct: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300

Query: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360
           + PE LAFDPNTGAGTIIDSGTVITRFV PVY AVRDEFRKQVGGSFSPLGAFDTCF TS
Sbjct: 301 IPPETLAFDPNTGAGTIIDSGTVITRFVDPVYTAVRDEFRKQVGGSFSPLGAFDTCFTTS 360

Query: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420
           NE++AP ITFH SGLDLKLPMENSLIHSSAG+LACLAMAAAPNNVNSVLNVIANLQQQNH
Sbjct: 361 NEMAAPGITFHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNH 420

Query: 421 RILFDVANSKLGIAREL 438
           RILFD+ANSKLGIAREL
Sbjct: 421 RILFDIANSKLGIAREL 435

BLAST of Moc08g02800 vs. NCBI nr
Match: XP_023547058.1 (aspartyl protease AED3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 754.6 bits (1947), Expect = 5.8e-214
Identity = 377/437 (86.27%), Postives = 402/437 (91.99%), Query Frame = 0

Query: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60
           M +K  F F LA  +L L SNASD C + +DGS D+SVIPIYGKCSPFTAPK+ESWVNTV
Sbjct: 1   MAVKFVFFFFLA--LLALNSNASDLCAAGSDGSGDLSVIPIYGKCSPFTAPKSESWVNTV 60

Query: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120
           ++MA+KD  R+KYL SL AQK+VAAPIASGQ  LNIGNYVVRVQLGTPGQ MYMVLDTS+
Sbjct: 61  IDMASKDPARIKYLSSLAAQKTVAAPIASGQHALNIGNYVVRVQLGTPGQAMYMVLDTSS 120

Query: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180
           DAAW PCSGC+GCSA TF SK SS+FATLDCSKP+CSQ RGLSCPTTGS +CLFNQTYGG
Sbjct: 121 DAAWAPCSGCSGCSATTFLSKNSSTFATLDCSKPQCSQARGLSCPTTGSVDCLFNQTYGG 180

Query: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240
           DSSFSATLVQD+LHLG DVIPNFSFGC+SSASGSSIPPQGL+GLGRGPLSLISQS SLYS
Sbjct: 181 DSSFSATLVQDTLHLGTDVIPNFSFGCISSASGSSIPPQGLLGLGRGPLSLISQSTSLYS 240

Query: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300
           GLFSYCLPSFKSYYFSGSLKLGPVGQPK+IRTTPLLKNPHRPSLYYVNLTGISVGRVLVP
Sbjct: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300

Query: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360
           + PE LAFDPNTGAGTIIDSGTVITRFV PVY AVRDEFRKQVGGSFSPLGAFDTCF TS
Sbjct: 301 IPPETLAFDPNTGAGTIIDSGTVITRFVYPVYTAVRDEFRKQVGGSFSPLGAFDTCFTTS 360

Query: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420
           NE++AP ITFH SGLDLKLPMENSLIHSSAG+LACLAMAAAPNNVNSVLNVIANLQQQNH
Sbjct: 361 NEMAAPGITFHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNH 420

Query: 421 RILFDVANSKLGIAREL 438
           RILFD+ANSKLGIAREL
Sbjct: 421 RILFDIANSKLGIAREL 435

BLAST of Moc08g02800 vs. NCBI nr
Match: KAG6598739.1 (Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 754.2 bits (1946), Expect = 7.6e-214
Identity = 376/437 (86.04%), Postives = 402/437 (91.99%), Query Frame = 0

Query: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60
           M +K  F F LA  +L L SNASD C + +DGS D+SVIPIYGKCSPFTAPK+ESWVNTV
Sbjct: 1   MAVKFVFFFFLA--LLALNSNASDLCAAGSDGSGDLSVIPIYGKCSPFTAPKSESWVNTV 60

Query: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120
           ++MA+KD  R+KYL SL AQK+VAAPIASGQ  LNIGNYVVRVQLGTPGQ MYMVLDTS+
Sbjct: 61  IDMASKDPARIKYLSSLAAQKTVAAPIASGQHALNIGNYVVRVQLGTPGQAMYMVLDTSS 120

Query: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180
           DAAW PCSGC+GCSA TF +K SS+FATLDCSKP+CSQ RGLSCPTTGS +CLFNQTYGG
Sbjct: 121 DAAWAPCSGCSGCSATTFLAKNSSTFATLDCSKPQCSQARGLSCPTTGSVDCLFNQTYGG 180

Query: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240
           DSSFSATLVQD+LHLG DVIPNFSFGC+SSASGSSIPPQGL+GLGRGPLSLISQS SLYS
Sbjct: 181 DSSFSATLVQDTLHLGSDVIPNFSFGCISSASGSSIPPQGLLGLGRGPLSLISQSTSLYS 240

Query: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300
           GLFSYCLPSFKSYYFSGSLKLGPVGQPK+IRTTPLLKNPHRPSLYYVNLTGISVGRVLVP
Sbjct: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300

Query: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360
           + PE LAFDPNTGAGTIIDSGTVITRFV PVY AVRDEFRKQVGGSFSPLGAFDTCF TS
Sbjct: 301 IPPETLAFDPNTGAGTIIDSGTVITRFVYPVYTAVRDEFRKQVGGSFSPLGAFDTCFTTS 360

Query: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420
           NE++AP ITFH SGLDLKLPMENSLIHSSAG+LACLAMAAAPNNVNSVLNVIANLQQQNH
Sbjct: 361 NEMAAPGITFHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNH 420

Query: 421 RILFDVANSKLGIAREL 438
           RILFD+ANSKLGIAREL
Sbjct: 421 RILFDIANSKLGIAREL 435

BLAST of Moc08g02800 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.7e-155
Identity = 283/440 (64.32%), Postives = 347/440 (78.86%), Query Frame = 0

Query: 6   YFLFVLAILILGLKSNASDPCPSKA-DGSDDISVIPIYGKCSPFTAPK-TESWVNTVMNM 65
           +F F+  +L     +   D C + A DGSDD+S+IPI  KCSPF     + S ++TV++M
Sbjct: 8   FFFFLTLLLPFTFTTATRDTCATAAPDGSDDLSIIPINAKCSPFAPTHVSASVIDTVLHM 67

Query: 66  AAKDAGRVKYLWSL--GAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSND 125
           A+ D+ R+ YL SL  G  K  + P+ASG Q L+IGNYVVR +LGTP Q+M+MVLDTSND
Sbjct: 68  ASSDSHRLTYLSSLVAGKPKPTSVPVASGNQ-LHIGNYVVRAKLGTPPQLMFMVLDTSND 127

Query: 126 AAWVPCSGCAGCS--AVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTG--SAECLFNQT 185
           A W+PCSGC+GCS  + +F++ +SS+++T+ CS  +C+Q RGL+CP++    + C FNQ+
Sbjct: 128 AVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSVCSFNQS 187

Query: 186 YGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGS 245
           YGGDSSFSA+LVQD+L L  DVIPNFSFGC++SASG+S+PPQGLMGLGRGP+SL+SQ+ S
Sbjct: 188 YGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVSQTTS 247

Query: 246 LYSGLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRV 305
           LYSG+FSYCLPSF+S+YFSGSLKLG +GQPK+IR TPLL+NP RPSLYYVNLTG+SVG V
Sbjct: 248 LYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGVSVGSV 307

Query: 306 LVPVQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVG-GSFSPLGAFDTC 365
            VPV P +L FD N+GAGTIIDSGTVITRF  PVY A+RDEFRKQV   SFS LGAFDTC
Sbjct: 308 QVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTLGAFDTC 367

Query: 366 FGTSNEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQ 425
           F   NE  AP IT H + LDLKLPMEN+LIHSSAG L CL+MA    N N+VLNVIANLQ
Sbjct: 368 FSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQ 427

Query: 426 QQNHRILFDVANSKLGIARE 437
           QQN RILFDV NS++GIA E
Sbjct: 428 QQNLRILFDVPNSRIGIAPE 446

BLAST of Moc08g02800 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.0e-108
Identity = 217/407 (53.32%), Postives = 286/407 (70.27%), Query Frame = 0

Query: 51  PKTESWVNTVMNMAAKDAGRVKYLWSLGAQKSV-AAPIASGQQVLNIGNYVVRVQLGTPG 110
           P + S + +++ +A  D  R+ +L S  A   V +AP+ASGQ      +YVVR  LG+P 
Sbjct: 33  PSSPSPLESIIALARDDDARLLFLSSKAATAGVSSAPVASGQAP---PSYVVRAGLGSPS 92

Query: 111 QVMYMVLDTSNDAAWVPCSGCAGC-SAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTT- 170
           Q + + LDTS DA W  CS C  C S+  F+   SSS+A+L CS   C   +G +CP   
Sbjct: 93  QQLLLALDTSADATWAHCSPCGTCPSSSLFAPANSSSYASLPCSSSWCPLFQGQACPAPQ 152

Query: 171 --GSA--------ECLFNQTYGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASG--SS 230
             G A         C F++ +  D+SF A L  D+L LG D IPN++FGC+SS +G  ++
Sbjct: 153 GGGDAAPPPATLPTCAFSKPF-ADASFQAALASDTLRLGKDAIPNYTFGCVSSVTGPTTN 212

Query: 231 IPPQGLMGLGRGPLSLISQSGSLYSGLFSYCLPSFKSYYFSGSLKLGP-VGQPKAIRTTP 290
           +P QGL+GLGRGP++L+SQ+GSLY+G+FSYCLPS++SYYFSGSL+LG   GQP+++R TP
Sbjct: 213 MPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLRLGAGGGQPRSVRYTP 272

Query: 291 LLKNPHRPSLYYVNLTGISVGRVLVPVQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAA 350
           +L+NPHR SLYYVN+TG+SVG   V V     AFD  TGAGT++DSGTVITR+ +PVYAA
Sbjct: 273 MLRNPHRSSLYYVNVTGLSVGHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAA 332

Query: 351 VRDEFRKQVG--GSFSPLGAFDTCFGTSNEVS--APSITFHF-SGLDLKLPMENSLIHSS 410
           +R+EFR+QV     ++ LGAFDTCF T    +  AP++T H   G+DL LPMEN+LIHSS
Sbjct: 333 LREEFRRQVAAPSGYTSLGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSS 392

Query: 411 AGALACLAMAAAPNNVNSVLNVIANLQQQNHRILFDVANSKLGIARE 437
           A  LACLAMA AP NVNSV+NVIANLQQQN R++FDVANS++G A+E
Sbjct: 393 ATPLACLAMAEAPQNVNSVVNVIANLQQQNIRVVFDVANSRVGFAKE 435

BLAST of Moc08g02800 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.3e-51
Identity = 136/395 (34.43%), Postives = 189/395 (47.85%), Query Frame = 0

Query: 66  KDAGRVKYLWSLGAQ----------------KSVAAPIASGQQVLNIGNYVVRVQLGTPG 125
           +D+ RVK + +L AQ                 SV + ++ G      G Y  R+ +GTP 
Sbjct: 98  RDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGS-----GEYFTRLGVGTPA 157

Query: 126 QVMYMVLDTSNDAAWVPCSGCAGC---SAVTFSSKTSSSFATLDCSKPECSQVRGLSCPT 185
           + +YMVLDT +D  W+ C+ C  C   S   F  + S ++AT+ CS P C ++    C  
Sbjct: 158 RYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGC-N 217

Query: 186 TGSAECLFNQTYGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGR 245
           T    CL+  +YG  S        ++L    + +   + GC     G  +   GL+GLG+
Sbjct: 218 TRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGK 277

Query: 246 GPLSLISQSGSLYSGLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYY 305
           G LS   Q+G  ++  FSYCL    +     S+  G     +  R TPLL NP   + YY
Sbjct: 278 GKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYY 337

Query: 306 VNLTGISVGRVLVP-VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGG 365
           V L GISVG   VP V       D     G IIDSGT +TR + P Y A+RD FR  VG 
Sbjct: 338 VGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFR--VGA 397

Query: 366 S----FSPLGAFDTCFGTS--NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMA 425
                      FDTCF  S  NEV  P++  HF G D+ LP  N LI        C A A
Sbjct: 398 KTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFA 457

Query: 426 AAPNNVNSVLNVIANLQQQNHRILFDVANSKLGIA 435
                    L++I N+QQQ  R+++D+A+S++G A
Sbjct: 458 GTMGG----LSIIGNIQQQGFRVVYDLASSRVGFA 480

BLAST of Moc08g02800 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.7e-47
Identity = 133/356 (37.36%), Postives = 181/356 (50.84%), Query Frame = 0

Query: 97  GNYVVRVQLGTPGQVMYMVLDTSNDAAWVPCSGCAGC---SAVTFSSKTSSSFATLDCSK 156
           G Y++ + +GTP Q    ++DT +D  W  C  C  C   S   F+ + SSSF+TL CS 
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSS 152

Query: 157 PECSQVRGLSCPTTGSAECLFNQTYGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASG 216
             C   + LS PT  +  C +   YG  S    ++  ++L  G   IPN +FGC  +  G
Sbjct: 153 QLC---QALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQG 212

Query: 217 -SSIPPQGLMGLGRGPLSLISQSGSLYSGLFSYCL----PSFKSYYFSGSLKLG-PVGQP 276
                  GL+G+GRGPLSL SQ   L    FSYC+     S  S    GSL      G P
Sbjct: 213 FGQGNGAGLVGMGRGPLSLPSQ---LDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSP 272

Query: 277 KAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPVQPEHLAFDPNTG-AGTIIDSGTVITR 336
                T L+++   P+ YY+ L G+SVG   +P+ P   A + N G  G IIDSGT +T 
Sbjct: 273 ----NTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 332

Query: 337 FVSPVYAAVRDEFRKQ-----VGGSFSPLGAFDTCFGTSNEVS---APSITFHFSGLDLK 396
           FV+  Y +VR EF  Q     V GS S    FD CF T ++ S    P+   HF G DL+
Sbjct: 333 FVNNAYQSVRQEFISQINLPVVNGSSS---GFDLCFQTPSDPSNLQIPTFVMHFDGGDLE 392

Query: 397 LPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNHRILFDVANSKLGIA 435
           LP EN  I  S G L CLAM ++       +++  N+QQQN  +++D  NS +  A
Sbjct: 393 LPSENYFISPSNG-LICLAMGSSSQG----MSIFGNIQQQNMLVVYDTGNSVVSFA 430

BLAST of Moc08g02800 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.7e-44
Identity = 114/344 (33.14%), Postives = 164/344 (47.67%), Query Frame = 0

Query: 97  GNYVVRVQLGTPGQVMYMVLDTSNDAAWVPCSGCAGC---SAVTFSSKTSSSFATLDCSK 156
           G Y VR+ +G+P +  YMV+D+ +D  WV C  C  C   S   F    S S+  + C  
Sbjct: 129 GEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGS 188

Query: 157 PECSQVRGLSCPTTGSAECLFNQTYGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASG 216
             C ++    C + G   C +   YG  S    TL  ++L     V+ N + GC     G
Sbjct: 189 SVCDRIENSGCHSGG---CRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRG 248

Query: 217 SSIPPQGLMGLGRGPLSLISQSGSLYSGLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTT 276
             I   GL+G+G G +S + Q      G F YCL S +    +GSL  G    P      
Sbjct: 249 MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVS-RGTDSTGSLVFGREALPVGASWV 308

Query: 277 PLLKNPHRPSLYYVNLTGISVGRVLVPVQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYA 336
           PL++NP  PS YYV L G+ VG V +P+             G ++D+GT +TR  +  Y 
Sbjct: 309 PLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYV 368

Query: 337 AVRDEFRKQVGG--SFSPLGAFDTCFGTSNEVS--APSITFHFS-GLDLKLPMENSLIHS 396
           A RD F+ Q       S +  FDTC+  S  VS   P+++F+F+ G  L LP  N L+  
Sbjct: 369 AFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPV 428

Query: 397 SAGALACLAMAAAPNNVNSVLNVIANLQQQNHRILFDVANSKLG 433
                 C A AA+P      L++I N+QQ+  ++ FD AN  +G
Sbjct: 429 DDSGTYCFAFAASPTG----LSIIGNIQQEGIQVSFDGANGFVG 464

BLAST of Moc08g02800 vs. ExPASy TrEMBL
Match: A0A6J1BTI5 (aspartyl protease AED3 OS=Momordica charantia OX=3673 GN=LOC111005199 PE=4 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.7e-248
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0

Query: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60
           MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV
Sbjct: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60

Query: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120
           MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN
Sbjct: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120

Query: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180
           DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG
Sbjct: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180

Query: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240
           DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS
Sbjct: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240

Query: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300
           GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP
Sbjct: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300

Query: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360
           VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS
Sbjct: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360

Query: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420
           NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH
Sbjct: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420

Query: 421 RILFDVANSKLGIAREL 438
           RILFDVANSKLGIAREL
Sbjct: 421 RILFDVANSKLGIAREL 437

BLAST of Moc08g02800 vs. ExPASy TrEMBL
Match: A0A6J1HAN7 (aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111462342 PE=4 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 2.2e-214
Identity = 376/437 (86.04%), Postives = 402/437 (91.99%), Query Frame = 0

Query: 1   MDLKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTV 60
           M +K  F F LA  +L L SNASD C + +DGS D+SVIPIYGKCSPFTAPK+ESWVNTV
Sbjct: 1   MAVKFVFFFFLA--LLALNSNASDLCAAGSDGSGDLSVIPIYGKCSPFTAPKSESWVNTV 60

Query: 61  MNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSN 120
           ++MA+KD  R+KYL SL AQK+VAAPIASGQ  LNIGNYVVRVQLGTPGQ MYMVLDTS+
Sbjct: 61  IDMASKDPARIKYLSSLAAQKTVAAPIASGQHALNIGNYVVRVQLGTPGQAMYMVLDTSS 120

Query: 121 DAAWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGG 180
           DAAW PCSGC+GCSA TF +K SS+FATLDCSKP+CSQ RGLSCPTTGS +CLFNQTYGG
Sbjct: 121 DAAWAPCSGCSGCSATTFLAKNSSTFATLDCSKPQCSQARGLSCPTTGSVDCLFNQTYGG 180

Query: 181 DSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYS 240
           DSSFSATLVQD+LHLG DVIPNFSFGC+SSASGSSIPPQGL+GLGRGPLSLISQS SLYS
Sbjct: 181 DSSFSATLVQDTLHLGSDVIPNFSFGCISSASGSSIPPQGLLGLGRGPLSLISQSTSLYS 240

Query: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300
           GLFSYCLPSFKSYYFSGSLKLGPVGQPK+IRTTPLLKNPHRPSLYYVNLTGISVGRVLVP
Sbjct: 241 GLFSYCLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLKNPHRPSLYYVNLTGISVGRVLVP 300

Query: 301 VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTS 360
           + PE LAFDPNTGAGTIIDSGTVITRFV PVY AVRDEFRKQVGGSFSPLGAFDTCF TS
Sbjct: 301 IPPETLAFDPNTGAGTIIDSGTVITRFVDPVYTAVRDEFRKQVGGSFSPLGAFDTCFTTS 360

Query: 361 NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNH 420
           NE++AP ITFH SGLDLKLPMENSLIHSSAG+LACLAMAAAPNNVNSVLNVIANLQQQNH
Sbjct: 361 NEMAAPGITFHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNH 420

Query: 421 RILFDVANSKLGIAREL 438
           RILFD+ANSKLGIAREL
Sbjct: 421 RILFDIANSKLGIAREL 435

BLAST of Moc08g02800 vs. ExPASy TrEMBL
Match: A0A6J1K469 (aspartyl protease AED3 OS=Cucurbita maxima OX=3661 GN=LOC111492140 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 6.3e-214
Identity = 373/435 (85.75%), Postives = 401/435 (92.18%), Query Frame = 0

Query: 3   LKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTVMN 62
           + + F F + + +L L SNASD C + +DGS D+SVIPIYGKCSPFTAPK+ESWVNTV++
Sbjct: 1   MAVKFFFFVFLALLALNSNASDLCAAGSDGSGDLSVIPIYGKCSPFTAPKSESWVNTVID 60

Query: 63  MAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSNDA 122
           MA+KD  R+KYL SL AQK+VAAPIASGQ  LNIGNYVVRVQLGTPGQ MYMVLDTS+DA
Sbjct: 61  MASKDPARIKYLSSLAAQKTVAAPIASGQHALNIGNYVVRVQLGTPGQAMYMVLDTSSDA 120

Query: 123 AWVPCSGCAGCSAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGGDS 182
           AW PCSGC+GCSA TF SK SS+FATLDCSKP+CSQ RGLSCPTTGS +CLFNQTYGGDS
Sbjct: 121 AWAPCSGCSGCSATTFLSKNSSTFATLDCSKPQCSQARGLSCPTTGSVDCLFNQTYGGDS 180

Query: 183 SFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYSGL 242
           SFSATLVQD+LHLG DVIPNFSFGC+SSASGSSIPPQGL+GLGRGPLSLISQS SLYSGL
Sbjct: 181 SFSATLVQDTLHLGTDVIPNFSFGCISSASGSSIPPQGLLGLGRGPLSLISQSTSLYSGL 240

Query: 243 FSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPVQ 302
           FSYCLPSFKSYYFSGSLKLGPVGQPK+IRTTPLLKNPHRPSLYYVNLTGISVGRVLVP+ 
Sbjct: 241 FSYCLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPIP 300

Query: 303 PEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTSNE 362
           PE LAFDPNTGAGTIIDSGTVITRFV PVY AVRDEFRKQVGGSFSPLGAFDTCF TSNE
Sbjct: 301 PETLAFDPNTGAGTIIDSGTVITRFVYPVYTAVRDEFRKQVGGSFSPLGAFDTCFTTSNE 360

Query: 363 VSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNHRI 422
           ++AP ITFH SGLDLKLPMENSLIHSSAG+LACLAMAAAPNNVNSVLNVIANLQQQNHRI
Sbjct: 361 MAAPGITFHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNHRI 420

Query: 423 LFDVANSKLGIAREL 438
           LFD+ANSKLGIAREL
Sbjct: 421 LFDIANSKLGIAREL 435

BLAST of Moc08g02800 vs. ExPASy TrEMBL
Match: A0A5A7VCS8 (Aspartyl protease AED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004150 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 6.5e-211
Identity = 369/436 (84.63%), Postives = 403/436 (92.43%), Query Frame = 0

Query: 3   LKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTVMN 62
           + + F FVL++ +L L SNA D C S +DG  D+SVIPIYGKCSPFTAPK+ESW+NTV++
Sbjct: 1   MALKFFFVLSLALLLLNSNAFDLCASGSDG--DLSVIPIYGKCSPFTAPKSESWMNTVID 60

Query: 63  MAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSNDA 122
           MA+KD  R++YL SL AQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQ MYMVLDTSNDA
Sbjct: 61  MASKDPARIRYLSSLTAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQTMYMVLDTSNDA 120

Query: 123 AWVPCSGCAGCSA-VTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGGD 182
           AW PCSGC GCS   TFS+K SS+FATLDCSKP+CSQ RGLSCPTTG+A+CLFNQTYGGD
Sbjct: 121 AWAPCSGCTGCSGPATFSAKNSSTFATLDCSKPQCSQARGLSCPTTGNADCLFNQTYGGD 180

Query: 183 SSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYSG 242
           SSFSATLV+DSLHLG +VIPNFSFGC+SSASGSSIPPQGLMGLGRGPLSLISQSGSLYSG
Sbjct: 181 SSFSATLVEDSLHLGSNVIPNFSFGCISSASGSSIPPQGLMGLGRGPLSLISQSGSLYSG 240

Query: 243 LFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPV 302
           +FSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLL+NPHRPSLYYVNLTGISVGRVLVP+
Sbjct: 241 IFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLRNPHRPSLYYVNLTGISVGRVLVPI 300

Query: 303 QPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTSN 362
            PE LAFDPNTGAGTIIDSGTVITRFV PVY A+RDEFRKQVGGSFSPLGAFDTCF TSN
Sbjct: 301 SPELLAFDPNTGAGTIIDSGTVITRFVPPVYTAIRDEFRKQVGGSFSPLGAFDTCFATSN 360

Query: 363 EVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNHR 422
           EVSAP++T H SGLDL+LPMENSLIHSSAG+LACLAMAAAPNNVN+V+NVIANLQQQNHR
Sbjct: 361 EVSAPAVTLHLSGLDLRLPMENSLIHSSAGSLACLAMAAAPNNVNAVVNVIANLQQQNHR 420

Query: 423 ILFDVANSKLGIAREL 438
           ILFD+ N+KLGIAREL
Sbjct: 421 ILFDINNAKLGIAREL 434

BLAST of Moc08g02800 vs. ExPASy TrEMBL
Match: A0A1S3BCB0 (aspartyl protease AED3 OS=Cucumis melo OX=3656 GN=LOC103488136 PE=4 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 6.5e-211
Identity = 369/436 (84.63%), Postives = 403/436 (92.43%), Query Frame = 0

Query: 3   LKIYFLFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTVMN 62
           + + F FVL++ +L L SNA D C S +DG  D+SVIPIYGKCSPFTAPK+ESW+NTV++
Sbjct: 1   MALKFFFVLSLALLLLNSNAFDLCASGSDG--DLSVIPIYGKCSPFTAPKSESWMNTVID 60

Query: 63  MAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSNDA 122
           MA+KD  R++YL SL AQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQ MYMVLDTSNDA
Sbjct: 61  MASKDPARIRYLSSLTAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQTMYMVLDTSNDA 120

Query: 123 AWVPCSGCAGCSA-VTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGGD 182
           AW PCSGC GCS   TFS+K SS+FATLDCSKP+CSQ RGLSCPTTG+A+CLFNQTYGGD
Sbjct: 121 AWAPCSGCTGCSGPATFSAKNSSTFATLDCSKPQCSQARGLSCPTTGNADCLFNQTYGGD 180

Query: 183 SSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYSG 242
           SSFSATLV+DSLHLG +VIPNFSFGC+SSASGSSIPPQGLMGLGRGPLSLISQSGSLYSG
Sbjct: 181 SSFSATLVEDSLHLGSNVIPNFSFGCISSASGSSIPPQGLMGLGRGPLSLISQSGSLYSG 240

Query: 243 LFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPV 302
           +FSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLL+NPHRPSLYYVNLTGISVGRVLVP+
Sbjct: 241 IFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLRNPHRPSLYYVNLTGISVGRVLVPI 300

Query: 303 QPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGSFSPLGAFDTCFGTSN 362
            PE LAFDPNTGAGTIIDSGTVITRFV PVY A+RDEFRKQVGGSFSPLGAFDTCF TSN
Sbjct: 301 SPELLAFDPNTGAGTIIDSGTVITRFVPPVYTAIRDEFRKQVGGSFSPLGAFDTCFATSN 360

Query: 363 EVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNHR 422
           EVSAP++T H SGLDL+LPMENSLIHSSAG+LACLAMAAAPNNVN+V+NVIANLQQQNHR
Sbjct: 361 EVSAPAVTLHLSGLDLRLPMENSLIHSSAGSLACLAMAAAPNNVNAVVNVIANLQQQNHR 420

Query: 423 ILFDVANSKLGIAREL 438
           ILFD+ N+KLGIAREL
Sbjct: 421 ILFDINNAKLGIAREL 434

BLAST of Moc08g02800 vs. TAIR 10
Match: AT1G09750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.2e-156
Identity = 283/440 (64.32%), Postives = 347/440 (78.86%), Query Frame = 0

Query: 6   YFLFVLAILILGLKSNASDPCPSKA-DGSDDISVIPIYGKCSPFTAPK-TESWVNTVMNM 65
           +F F+  +L     +   D C + A DGSDD+S+IPI  KCSPF     + S ++TV++M
Sbjct: 8   FFFFLTLLLPFTFTTATRDTCATAAPDGSDDLSIIPINAKCSPFAPTHVSASVIDTVLHM 67

Query: 66  AAKDAGRVKYLWSL--GAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSND 125
           A+ D+ R+ YL SL  G  K  + P+ASG Q L+IGNYVVR +LGTP Q+M+MVLDTSND
Sbjct: 68  ASSDSHRLTYLSSLVAGKPKPTSVPVASGNQ-LHIGNYVVRAKLGTPPQLMFMVLDTSND 127

Query: 126 AAWVPCSGCAGCS--AVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTG--SAECLFNQT 185
           A W+PCSGC+GCS  + +F++ +SS+++T+ CS  +C+Q RGL+CP++    + C FNQ+
Sbjct: 128 AVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSVCSFNQS 187

Query: 186 YGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGS 245
           YGGDSSFSA+LVQD+L L  DVIPNFSFGC++SASG+S+PPQGLMGLGRGP+SL+SQ+ S
Sbjct: 188 YGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVSQTTS 247

Query: 246 LYSGLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRV 305
           LYSG+FSYCLPSF+S+YFSGSLKLG +GQPK+IR TPLL+NP RPSLYYVNLTG+SVG V
Sbjct: 248 LYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGVSVGSV 307

Query: 306 LVPVQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVG-GSFSPLGAFDTC 365
            VPV P +L FD N+GAGTIIDSGTVITRF  PVY A+RDEFRKQV   SFS LGAFDTC
Sbjct: 308 QVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTLGAFDTC 367

Query: 366 FGTSNEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQ 425
           F   NE  AP IT H + LDLKLPMEN+LIHSSAG L CL+MA    N N+VLNVIANLQ
Sbjct: 368 FSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQ 427

Query: 426 QQNHRILFDVANSKLGIARE 437
           QQN RILFDV NS++GIA E
Sbjct: 428 QQNLRILFDVPNSRIGIAPE 446

BLAST of Moc08g02800 vs. TAIR 10
Match: AT3G54400.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 441.8 bits (1135), Expect = 7.8e-124
Identity = 244/431 (56.61%), Postives = 298/431 (69.14%), Query Frame = 0

Query: 8   LFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTVMNMAAKD 67
           L +L I +L LKS + + C  K+  S D+ V  I   CSPF    + SW +T++    +D
Sbjct: 5   LLILLISLLILKSESIN-CNEKSH-SSDLRVFHINSLCSPFKT--SVSWADTLL----QD 64

Query: 68  AGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSNDAAWVPC 127
             R  YL SL   +  + PIASG+ ++    Y+VR  +GTP Q M + LDTSNDAAW+PC
Sbjct: 65  KARFLYLSSLAGVRKSSVPIASGRAIVQSPTYIVRANIGTPAQPMLVALDTSNDAAWIPC 124

Query: 128 SGCAGC-SAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGGDSSFSA 187
           SGC GC S+V F    SSS  TL C  P+C Q    SC  T S  C FN TYGG S+  A
Sbjct: 125 SGCVGCSSSVLFDPSKSSSSRTLQCEAPQCKQAPNPSC--TVSKSCGFNMTYGG-STIEA 184

Query: 188 TLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLISQSGSLYSGLFSYC 247
            L QD+L L  DVIPN++FGC++ ASG+S+P QGLMGLGRGPLSLISQS +LY   FSYC
Sbjct: 185 YLTQDTLTLASDVIPNYTFGCINKASGTSLPAQGLMGLGRGPLSLISQSQNLYQSTFSYC 244

Query: 248 LPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPVQPEHL 307
           LP+ KS  FSGSL+LGP  QP  I+TTPLLKNP R SLYYVNL GI VG  +V +    L
Sbjct: 245 LPNSKSSNFSGSLRLGPKNQPIRIKTTPLLKNPRRSSLYYVNLVGIRVGNKIVDIPTSAL 304

Query: 308 AFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQV-GGSFSPLGAFDTCFGTSNEVSA 367
           AFDP TGAGTI DSGTV TR V P Y AVR+EFR++V   + + LG FDTC+  S  V  
Sbjct: 305 AFDPATGAGTIFDSGTVYTRLVEPAYVAVRNEFRRRVKNANATSLGGFDTCY--SGSVVF 364

Query: 368 PSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNHRILFD 427
           PS+TF F+G+++ LP +N LIHSSAG L+CLAMAAAP NVNSVLNVIA++QQQNHR+L D
Sbjct: 365 PSVTFMFAGMNVTLPPDNLLIHSSAGNLSCLAMAAAPVNVNSVLNVIASMQQQNHRVLID 422

Query: 428 VANSKLGIARE 437
           V NS+LGI+RE
Sbjct: 425 VPNSRLGISRE 422

BLAST of Moc08g02800 vs. TAIR 10
Match: AT5G07030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 417.2 bits (1071), Expect = 2.0e-116
Identity = 223/435 (51.26%), Postives = 293/435 (67.36%), Query Frame = 0

Query: 8   LFVLAILILGLKSNASDPCPSKADGSDDISVIPIYGKCSPFTAPKTESWVNTVMNMAAKD 67
           LF +  L LGL     D   ++  GS  + +  I   CSPF +    SW   V+   A+D
Sbjct: 26  LFSILPLALGLNHPNCDLTKTQDQGS-TLRIFHIDSPCSPFKSSSPLSWEARVLQTLAQD 85

Query: 68  AGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVLDTSNDAAWVPC 127
             R++YL SL A +SV  PIASG+Q+L    Y+V+  +GTP Q + + +DTS+D AW+PC
Sbjct: 86  QARLQYLSSLVAGRSV-VPIASGRQMLQSTTYIVKALIGTPAQPLLLAMDTSSDVAWIPC 145

Query: 128 SGCAGC-SAVTFSSKTSSSFATLDCSKPECSQVRGLSCPTTGSAECLFNQTYGGDSSFSA 187
           SGC GC S   FS   S+SF  + CS P+C QV     PT G+  C FN TY G SS +A
Sbjct: 146 SGCVGCPSNTAFSPAKSTSFKNVSCSAPQCKQVPN---PTCGARACSFNLTY-GSSSIAA 205

Query: 188 TLVQDSLHLGGDVIPNFSFGCLSSASGSSI--PPQGLMGLGRGPLSLISQSGSLYSGLFS 247
            L QD++ L  D I  F+FGC++  +G     PPQGL+GLGRGPLSL+SQ+ S+Y   FS
Sbjct: 206 NLSQDTIRLAADPIKAFTFGCVNKVAGGGTIPPPQGLLGLGRGPLSLMSQAQSIYKSTFS 265

Query: 248 YCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVNLTGISVGRVLVPVQPE 307
           YCLPSF+S  FSGSL+LGP  QP+ ++ T LL+NP R SLYYVNL  I VGR +V + P 
Sbjct: 266 YCLPSFRSLTFSGSLRLGPTSQPQRVKYTQLLRNPRRSSLYYVNLVAIRVGRKVVDLPPA 325

Query: 308 HLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGS---FSPLGAFDTCFGTSN 367
            +AF+P+TGAGTI DSGTV TR   PVY AVR+EFRK+V  +    + LG FDTC+  S 
Sbjct: 326 AIAFNPSTGAGTIFDSGTVYTRLAKPVYEAVRNEFRKRVKPTTAVVTSLGGFDTCY--SG 385

Query: 368 EVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAAPNNVNSVLNVIANLQQQNHR 427
           +V  P+ITF F G+++ +P +N ++HS+AG+ +CLAMAAAP NVNSV+NVIA++QQQNHR
Sbjct: 386 QVKVPTITFMFKGVNMTMPADNLMLHSTAGSTSCLAMAAAPENVNSVVNVIASMQQQNHR 445

Query: 428 ILFDVANSKLGIARE 437
           +L DV N +LG+ARE
Sbjct: 446 VLIDVPNGRLGLARE 452

BLAST of Moc08g02800 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 205.7 bits (522), Expect = 9.4e-53
Identity = 136/395 (34.43%), Postives = 189/395 (47.85%), Query Frame = 0

Query: 66  KDAGRVKYLWSLGAQ----------------KSVAAPIASGQQVLNIGNYVVRVQLGTPG 125
           +D+ RVK + +L AQ                 SV + ++ G      G Y  R+ +GTP 
Sbjct: 98  RDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGS-----GEYFTRLGVGTPA 157

Query: 126 QVMYMVLDTSNDAAWVPCSGCAGC---SAVTFSSKTSSSFATLDCSKPECSQVRGLSCPT 185
           + +YMVLDT +D  W+ C+ C  C   S   F  + S ++AT+ CS P C ++    C  
Sbjct: 158 RYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGC-N 217

Query: 186 TGSAECLFNQTYGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGR 245
           T    CL+  +YG  S        ++L    + +   + GC     G  +   GL+GLG+
Sbjct: 218 TRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGK 277

Query: 246 GPLSLISQSGSLYSGLFSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYY 305
           G LS   Q+G  ++  FSYCL    +     S+  G     +  R TPLL NP   + YY
Sbjct: 278 GKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYY 337

Query: 306 VNLTGISVGRVLVP-VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGG 365
           V L GISVG   VP V       D     G IIDSGT +TR + P Y A+RD FR  VG 
Sbjct: 338 VGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFR--VGA 397

Query: 366 S----FSPLGAFDTCFGTS--NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMA 425
                      FDTCF  S  NEV  P++  HF G D+ LP  N LI        C A A
Sbjct: 398 KTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFA 457

Query: 426 AAPNNVNSVLNVIANLQQQNHRILFDVANSKLGIA 435
                    L++I N+QQQ  R+++D+A+S++G A
Sbjct: 458 GTMGG----LSIIGNIQQQGFRVVYDLASSRVGFA 480

BLAST of Moc08g02800 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 197.6 bits (501), Expect = 2.6e-50
Identity = 136/391 (34.78%), Postives = 186/391 (47.57%), Query Frame = 0

Query: 57  VNTVMNMAAKDAGRVKYLWSLGAQKSVAAPIASGQQVLNIGNYVVRVQLGTPGQVMYMVL 116
           V ++ ++AA   GR     +       +  + SG      G Y +R+ +GTP   +YMVL
Sbjct: 94  VKSITSLAAVSTGRNATKRTPRTAGGFSGAVISGLS-QGSGEYFMRLGVGTPATNVYMVL 153

Query: 117 DTSNDAAWVPCSGCAGCSAVT---FSSKTSSSFATLDCSKPECSQVRGLS-CPTTGSAEC 176
           DT +D  W+ CS C  C   T   F  K S +FAT+ C    C ++   S C T  S  C
Sbjct: 154 DTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTC 213

Query: 177 LFNQTYGGDSSFSATLVQDSLHLGGDVIPNFSFGCLSSASGSSIPPQGLMGLGRGPLSLI 236
           L+  +YG  S        ++L   G  + +   GC     G  +   GL+GLGRG LS  
Sbjct: 214 LYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFP 273

Query: 237 SQSGSLYSGLFSYCL----PSFKSYYFSGSLKLGPVGQPKAIRTTPLLKNPHRPSLYYVN 296
           SQ+ + Y+G FSYCL     S  S     ++  G    PK    TPLL NP   + YY+ 
Sbjct: 274 SQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQ 333

Query: 297 LTGISVGRVLVP-VQPEHLAFDPNTGAGTIIDSGTVITRFVSPVYAAVRDEFRKQVGGS- 356
           L GISVG   VP V       D     G IIDSGT +TR   P Y A+RD FR  +G + 
Sbjct: 334 LLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFR--LGATK 393

Query: 357 ---FSPLGAFDTCFGTS--NEVSAPSITFHFSGLDLKLPMENSLIHSSAGALACLAMAAA 416
                    FDTCF  S    V  P++ FHF G ++ LP  N LI  +     C A A  
Sbjct: 394 LKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAFAGT 453

Query: 417 PNNVNSVLNVIANLQQQNHRILFDVANSKLG 433
             +    L++I N+QQQ  R+ +D+  S++G
Sbjct: 454 MGS----LSIIGNIQQQGFRVAYDLVGSRVG 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132317.13.6e-248100.00aspartyl protease AED3 [Momordica charantia][more]
XP_038886172.16.6e-21888.10aspartyl protease AED3 [Benincasa hispida][more]
XP_022961622.14.5e-21486.04aspartyl protease AED3 [Cucurbita moschata][more]
XP_023547058.15.8e-21486.27aspartyl protease AED3 [Cucurbita pepo subsp. pepo][more]
KAG6598739.17.6e-21486.04Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
O044961.7e-15564.32Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1[more]
Q6F4N52.0e-10853.32Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1[more]
Q9LNJ31.3e-5134.43Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C35.7e-4737.36Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q9LHE32.7e-4433.14Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Match NameE-valueIdentityDescription
A0A6J1BTI51.7e-248100.00aspartyl protease AED3 OS=Momordica charantia OX=3673 GN=LOC111005199 PE=4 SV=1[more]
A0A6J1HAN72.2e-21486.04aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111462342 PE=4 SV=1[more]
A0A6J1K4696.3e-21485.75aspartyl protease AED3 OS=Cucurbita maxima OX=3661 GN=LOC111492140 PE=4 SV=1[more]
A0A5A7VCS86.5e-21184.63Aspartyl protease AED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A1S3BCB06.5e-21184.63aspartyl protease AED3 OS=Cucumis melo OX=3656 GN=LOC103488136 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09750.11.2e-15664.32Eukaryotic aspartyl protease family protein [more]
AT3G54400.17.8e-12456.61Eukaryotic aspartyl protease family protein [more]
AT5G07030.12.0e-11651.26Eukaryotic aspartyl protease family protein [more]
AT1G01300.19.4e-5334.43Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.6e-5034.78Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 264..444
e-value: 2.2E-45
score: 156.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 76..262
e-value: 5.0E-35
score: 123.2
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 95..438
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 99..262
e-value: 1.3E-33
score: 116.7
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 285..434
e-value: 7.6E-32
score: 110.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 493..534
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 482..534
NoneNo IPR availablePANTHERPTHR13683:SF839ASPARTYL PROTEASE AED3-LIKEcoord: 10..437
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 10..437
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 99..434
score: 36.442677

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g02800.1Moc08g02800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0043067 regulation of programmed cell death
molecular_function GO:0004190 aspartic-type endopeptidase activity