Moc08g02680 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g02680
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionArgininosuccinate lyase
Locationchr8: 1874343 .. 1878899 (+)
RNA-Seq ExpressionMoc08g02680
SyntenyMoc08g02680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGAACTCGATGTTTCGTCCGCCCCTGAGGCTGCCGGAGCAACGTCCGCGGCCGGCTCCTCTGTCTCCAAGAAGGCAGAGACCAAAGAAATTAAGCTATGGGGCGGTCGCTTCCAGGAGAGCGTCACCGACGCCGTTGAACGCTTCACGGAGTCGATCTCGTACGATAAGGATCTTTACAAGCATGACATCAGTGGCAGTCGCGCTCATGCATCCATGCTCGCTAAGCAGGTCTATTCTGTCACCTCTCATTTTCATTTTGGTGATTTCTGTTCAGCTCTATTCTGTTTTGTTTCCGGAGAGGAATGTTTACTACTCATTTATGGCGTTTGTACTAACCTTATGAGGCTGAATCACAATGATGAGCTGAATTTTAACTCAATTTTGATCTATTATATCCTGGATTTTTCTCGGTTGCTATGCGAATTCATGATTATGGTTCGTGATTTCGGTTGCAGGGATTGATGAGTATCAGTGACAGGGACAGTATTCTAGCAGGTCTCGATGAGATCGAAAGGCGGATTCGAAACGGTGAGTTCGTCTGGAGAGCTGATAGGGAAGATGTTCACATGAACATTGAAGCCGCACTTACTGATTTGATTGGCGAACCTGCCAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTTGCGACTGATTTTCGCTTGTGGTGCCGCGATGCAATTGATTCGATCGCTGAGAGGATTAAAGATCTTCAGGTTGCAATTCTGGAATTGGCAATTAAGAATGCAGGAATTATTGTTCCAGGTTATACGCACATGCAGAGAGCACAACCCGTCCTACTTCAGCATCTTCTCTTATCATTCGTTGAGCAGGTATTTAACTACTATTCAAGGTCATCATCTTAAGTCGTGGCTGAAATCCTAAAATAAATTCTTGCTGTTCTGCTGGTAGTGATAGCTGCATGATTTCCATATTGCCTGAAACTAGAGATGAGAGTAATGGGACTTATTAACTGCATCCTTGACAAAGGAGATTGTGTTTGTCTGTGACTGCTTGGTCTACCTTAAACTTCTTGGAGGCGAAGCATCAGAAATTCTCCCCATACTTTCTCCATGGAAGCTTTGAACATTTCACTTGAATGGACAGTCCTCTTCATAAAACATTCTCCACATATTTTAACTTGATTTTAAAAGACATTCCTTCTTCACAGTCATAAACTCCCTTTAGAGACGGACTAGTGATGGTTGCTTATAGAACTATATAATGTTCTACATCATTTATCATTTGCATAATGGTTTTCCTTTTCCTTAGCTTGAGGAAAAAATCCTAAATTAGGATCAAAGATCTTTCTAGTACATCACTTTTACTAGAACCTACAAAACACTGATTCTGGACAGATGTTGTTACACGTTTTGTAGCTTGATCGGGATGCTGGTCGTTTACTGGACTGCAGAGTTAGGCTGAATTTCTGCCCCTTAGGAGCGTGTGCATTGGCTGGTACTGGCCTCCCAATTGATCGATTCATGACTTCAAGTGCTCTAGGATTCTCATCTCCTTTGAGAAACAGGTTTGTTGTCTAGTATTTTTCATAAAATTCACCGAAGGAATAGGATTTCTCATTGATTGATGTTTAGTCTCTGTATTTATACACTTGAGCATTTACAAACAGAAATTGGTTTATTGATATAACAATCCAACCAATTCTCAATTATAGAGACTCTACAGAAAGCTAGATACAAATAACAAACACTCAAAAAATGACTCGATCTACTGCCTTAACAATTTTTACTTGTCAAGGTTGTTAAAAGTAGTGATCCCGATGGTGAGAATATCTATGTTTTTCCCTCTGCAGGGTGTAATTTCGCACTTTCTCCCCTCCCGTGGTTTTTAGGGGAATCTGGAATAGATGTTAGGTCACTTGCACGTTATATGACTTTAATCTAAACTGTAGCAACATCATCGTGGATTTCCAAATTCCTATTTGTGATTACAAGTTGCTGGAGGAGAATTCTATTACCTCATTTTCAAACTTCATTTACACTCTTACTTCTTCTAAACCTTTGATCACATGGGTTATATAAACTAGCGTCGGTTTTCAAGCATTAAACTTGCTCGAATGGGCTTTTTGTGGCTTGGAGTAGCTTATTTCCTTTTGCGTATCTGTAACTCATCCTTCTGTAATTCCTTGTATTTTTTTCATCTTAGTCTGTTGAATAACTTGTAAATTTTGTTGTCTCGAGTAGTATTGATGCTGTTTCAGACCGGGACTTCGCTATGGAATTTCTTTCTGCCAATTCTATTACAGCCGTTCATCTTTCTCGTCTGGGGGAAGAATGGGTGCTGTGGTCCTCCGAGGAGTTTGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCGGATCCGATGGAACTTGTTCGTGGAAAGTCTGGTAGAGTCATAGGTGATTTAGTAACCCTTCTAACACTATGCAAAGGGCTTCCACTTGCATACAACCGTGATCTACAGGTTTGAACTTTTTGACAGCTCAAATTCCCTTTTCATGAAACTTCTTTCACCTTCCTTTTGATTGAATAAGCTGTTTGTGTTGAGACAGGAAGATAAGGAACCTGTATTTGATAGTGTTAAGGCAGTTTCAGGGATGCTTGAAGTTTCAGCTGAGTTTGCTCGGAACATCAGTTTCAATCAGGACAGAATAAAGAAGGCCTTACCTGCTGGTCATCTTGATGCGACCACACTTGCGGATTATCTCGTGCATAAGGTTAGTATATTGTTTTACTTTTCCTTGTTAGAAGAGCTTAAATTTCAGAGTATTTTAAAATATTGATTTTGCTTGCAAAAGAGAGACTGATTTCCACTTCCCTAGTCCCTACATCATTTTTTTTTTATAATTATTTCCTCTGCATCTTATTTTTTATTACTCTTTTTTGGGGAGGGGGTGTTCAAAACTATGTAGCTCATGAGCATGGATGTTGGAGACGAGGTGGGGGTTTGAAAATCACAGAAGGCTTTTGACTTGCAAAATTCTAAACACAATTGATAATTGAGAACTTTGAAGAATTTACAGCATAGATTTTTCTCTTAAAAATTTTAGATAAAAGATTGATAGTTGAAGACTCTGAAAAGTTGCAAGCAAACATAAACGACCAAAGAAATTAGAGGCGACTTAATTAAGGATAATAAAACTCAAAATTTTGCTTCTAGAAGTTAGTTTAATGCAATAGTCCAGATCTCCCAGCCCCTCCCTGTATTCCATCAATAAACTGTGTTAATATTTATCTATATAATCTGGACCGGAAAAAAATTGGATTAAATACTATTTTTGGTTTTTATACTTTGACTTTTATTAATTTTAGTTCAAGTATGAAACGGTTGTTTTGGACCCTATACTTTGAAAAAGTGGCTATTTTAGTCCCTCCATTATTTTTTATCTCATTTATTGGATCAAAATTTTAGCTTACTTGCGATTTTTGTTTTAGTACAATATGTGGAGTTTGAACCCATGACCTTTTGGTCGGAGATAGATGCTTTAACTAGTTATGCTCAAATTAGCTTACTAAATTTGTATCAAAGAATTTCTATTGTGTATAAAATTTATGTTAACATTTTAAAAATAGGAACTAAAATGATACTTTCTTAAAAGTACTGGAACCAAAATAGACGTATTAGACGTATTGCAATTATATAGACTAAAATGAACTAAAAAATTAAAATATAAAGACTAAAATGATCCTTTTAAAGTACGAATACCAAGATGAAAATAGCCGGGAAACCAAAAAAAATATGATGAGACATAATGTACATAAATCTTTTCTGCTTTGGTTCTTAGCTTAGCAATTAGCGTGCATTTCCCACGAAGTTCGTGCATGAAGCACGTGTATATTTCACATTGTATCAGTTAACAAAGATTCTTTCAAGGACCTCCAAATGTTGCAATTTGCAGCAGGCTCTCATAAAATAGGGCCTTCAACGTACGACCATGTGCTGCCCCAACGGCCACATGGTCGGCTCTAACAATTCTCGAGGGCTTATTTGGCAGTTGTTCTCAGAACAGTTCTTTTTATATTTGTTCTAGAAACTGTTTTCTAGAACATTTTTTAGTTGATTTGTAAAACCAAAGACTATTTTGTGTTAAAAATAACATTATTTATAAGTTTAGGCAAGAAAACAATCTCCATTATTTTAATAGATATGTCATCCATGAAATGAACAAAAAGAAAACAAAATGAACAACAGTTGTTCAAAACACTTTTTACTGAACTTTTATGGACATTATGATTCTCTGATTTCATTTCCCCTTTTTGATAGGGAATACCTTTCAGAACATCTCATGATATTGTAGGCAAGTCCGTTGCCATGTGCCTTGGAAGGAACTGCCAGCTGAAGGACCTGAGTCTCGATGATCTGCGGACGTTGAGTCCAATCTTCGACGACGATGTTTATGAATTTCTTGGAGTAGAAAATGCAGTCAACAAATTTTCATCGTATGGCTCCACAGGATCAGAATGTGTTGCCAGTCAACTCGAGCATTGGATCGAAAAACTTGGCATTAGTCAGTAG

mRNA sequence

ATGACTGAACTCGATGTTTCGTCCGCCCCTGAGGCTGCCGGAGCAACGTCCGCGGCCGGCTCCTCTGTCTCCAAGAAGGCAGAGACCAAAGAAATTAAGCTATGGGGCGGTCGCTTCCAGGAGAGCGTCACCGACGCCGTTGAACGCTTCACGGAGTCGATCTCGTACGATAAGGATCTTTACAAGCATGACATCAGTGGCAGTCGCGCTCATGCATCCATGCTCGCTAAGCAGGGATTGATGAGTATCAGTGACAGGGACAGTATTCTAGCAGGTCTCGATGAGATCGAAAGGCGGATTCGAAACGGTGAGTTCGTCTGGAGAGCTGATAGGGAAGATGTTCACATGAACATTGAAGCCGCACTTACTGATTTGATTGGCGAACCTGCCAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTTGCGACTGATTTTCGCTTGTGGTGCCGCGATGCAATTGATTCGATCGCTGAGAGGATTAAAGATCTTCAGGTTGCAATTCTGGAATTGGCAATTAAGAATGCAGGAATTATTGTTCCAGGTTATACGCACATGCAGAGAGCACAACCCGTCCTACTTCAGCATCTTCTCTTATCATTCGTTGAGCAGCTTGATCGGGATGCTGGTCGTTTACTGGACTGCAGAGTTAGGCTGAATTTCTGCCCCTTAGGAGCGTGTGCATTGGCTGGTACTGGCCTCCCAATTGATCGATTCATGACTTCAAGTGCTCTAGGATTCTCATCTCCTTTGAGAAACAGTATTGATGCTGTTTCAGACCGGGACTTCGCTATGGAATTTCTTTCTGCCAATTCTATTACAGCCGTTCATCTTTCTCGTCTGGGGGAAGAATGGGTGCTGTGGTCCTCCGAGGAGTTTGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCGGATCCGATGGAACTTGTTCGTGGAAAGTCTGGTAGAGTCATAGGTGATTTAGTAACCCTTCTAACACTATGCAAAGGGCTTCCACTTGCATACAACCGTGATCTACAGGAAGATAAGGAACCTGTATTTGATAGTGTTAAGGCAGTTTCAGGGATGCTTGAAGTTTCAGCTGAGTTTGCTCGGAACATCAGTTTCAATCAGGACAGAATAAAGAAGGCCTTACCTGCTGGTCATCTTGATGCGACCACACTTGCGGATTATCTCGTGCATAAGGGAATACCTTTCAGAACATCTCATGATATTGTAGGCAAGTCCGTTGCCATGTGCCTTGGAAGGAACTGCCAGCTGAAGGACCTGAGTCTCGATGATCTGCGGACGTTGAGTCCAATCTTCGACGACGATGTTTATGAATTTCTTGGAGTAGAAAATGCAGTCAACAAATTTTCATCGTATGGCTCCACAGGATCAGAATGTGTTGCCAGTCAACTCGAGCATTGGATCGAAAAACTTGGCATTAGTCAGTAG

Coding sequence (CDS)

ATGACTGAACTCGATGTTTCGTCCGCCCCTGAGGCTGCCGGAGCAACGTCCGCGGCCGGCTCCTCTGTCTCCAAGAAGGCAGAGACCAAAGAAATTAAGCTATGGGGCGGTCGCTTCCAGGAGAGCGTCACCGACGCCGTTGAACGCTTCACGGAGTCGATCTCGTACGATAAGGATCTTTACAAGCATGACATCAGTGGCAGTCGCGCTCATGCATCCATGCTCGCTAAGCAGGGATTGATGAGTATCAGTGACAGGGACAGTATTCTAGCAGGTCTCGATGAGATCGAAAGGCGGATTCGAAACGGTGAGTTCGTCTGGAGAGCTGATAGGGAAGATGTTCACATGAACATTGAAGCCGCACTTACTGATTTGATTGGCGAACCTGCCAAGAAACTGCATACTGCTCGGAGCCGCAACGACCAGGTTGCGACTGATTTTCGCTTGTGGTGCCGCGATGCAATTGATTCGATCGCTGAGAGGATTAAAGATCTTCAGGTTGCAATTCTGGAATTGGCAATTAAGAATGCAGGAATTATTGTTCCAGGTTATACGCACATGCAGAGAGCACAACCCGTCCTACTTCAGCATCTTCTCTTATCATTCGTTGAGCAGCTTGATCGGGATGCTGGTCGTTTACTGGACTGCAGAGTTAGGCTGAATTTCTGCCCCTTAGGAGCGTGTGCATTGGCTGGTACTGGCCTCCCAATTGATCGATTCATGACTTCAAGTGCTCTAGGATTCTCATCTCCTTTGAGAAACAGTATTGATGCTGTTTCAGACCGGGACTTCGCTATGGAATTTCTTTCTGCCAATTCTATTACAGCCGTTCATCTTTCTCGTCTGGGGGAAGAATGGGTGCTGTGGTCCTCCGAGGAGTTTGGATTTATCACACCTAATGACGCTGTTTCAACTGGAAGTAGCATAATGCCTCAGAAGAAAAATCCGGATCCGATGGAACTTGTTCGTGGAAAGTCTGGTAGAGTCATAGGTGATTTAGTAACCCTTCTAACACTATGCAAAGGGCTTCCACTTGCATACAACCGTGATCTACAGGAAGATAAGGAACCTGTATTTGATAGTGTTAAGGCAGTTTCAGGGATGCTTGAAGTTTCAGCTGAGTTTGCTCGGAACATCAGTTTCAATCAGGACAGAATAAAGAAGGCCTTACCTGCTGGTCATCTTGATGCGACCACACTTGCGGATTATCTCGTGCATAAGGGAATACCTTTCAGAACATCTCATGATATTGTAGGCAAGTCCGTTGCCATGTGCCTTGGAAGGAACTGCCAGCTGAAGGACCTGAGTCTCGATGATCTGCGGACGTTGAGTCCAATCTTCGACGACGATGTTTATGAATTTCTTGGAGTAGAAAATGCAGTCAACAAATTTTCATCGTATGGCTCCACAGGATCAGAATGTGTTGCCAGTCAACTCGAGCATTGGATCGAAAAACTTGGCATTAGTCAGTAG

Protein sequence

MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGIIVPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRFMTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLEHWIEKLGISQ
Homology
BLAST of Moc08g02680 vs. NCBI nr
Match: XP_022131833.1 (argininosuccinate lyase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 968.4 bits (2502), Expect = 2.4e-278
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

Query: 481 HWIEKLGISQ 491
           HWIEKLGISQ
Sbjct: 481 HWIEKLGISQ 490

BLAST of Moc08g02680 vs. NCBI nr
Match: XP_038886091.1 (argininosuccinate lyase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 910.6 bits (2352), Expect = 5.8e-261
Identity = 455/488 (93.24%), Postives = 471/488 (96.52%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT+LDV SA +  GATSAAGS  +KK   KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTKLDVPSASQPTGATSAAGSCATKKPGAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDISGSRAHASMLAKQGLMSISDRD+ILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDISGSRAHASMLAKQGLMSISDRDNILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI ERIKD QVA+++LAIKNAGII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAIKNAGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLL+FVEQLDRDAGRLLDCR RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSAL FSSPLRNSIDAVSDRDFAMEFLSANSI A+HLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALEFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLSLD+LRTLSPIFDDDVYEFLGVEN+VNKFSSYGSTGSECVA+QL+
Sbjct: 421 SVAMCLGKNCQLKDLSLDELRTLSPIFDDDVYEFLGVENSVNKFSSYGSTGSECVANQLQ 480

Query: 481 HWIEKLGI 489
           +WIEKLGI
Sbjct: 481 YWIEKLGI 488

BLAST of Moc08g02680 vs. NCBI nr
Match: XP_023546913.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899.4 bits (2323), Expect = 1.3e-257
Identity = 453/490 (92.45%), Postives = 468/490 (95.51%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT  DVSSA EA G TSAA    +KK E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTIFDVSSAAEATGETSAA----AKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           +KHDISGSRAHASMLAKQGLMSISDRD ILAGLDEIE+RIRNGEFVWRADREDVHMNIEA
Sbjct: 61  FKHDISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI ERIKD QVA+++LAIKN GII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIIERIKDFQVAMVDLAIKNKGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLL+FVEQLDRDAGRLLDCR RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSI A+HLSRLGEEWVLWSSEEFGFI P+
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPS 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGDLVTLL+LCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLSL +LR LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGKNCQLKDLSLAELRALSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

Query: 481 HWIEKLGISQ 491
           +WIEKLGISQ
Sbjct: 481 YWIEKLGISQ 486

BLAST of Moc08g02680 vs. NCBI nr
Match: XP_022997120.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 896.7 bits (2316), Expect = 8.7e-257
Identity = 451/490 (92.04%), Postives = 467/490 (95.31%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT  DVSSA EA G TSAA    +KK E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTIFDVSSAAEATGETSAA----AKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           +KHDISGSRAHASMLAKQGLMSISDRD ILAGLDEIE+RI NGEFVWRADREDVHMNIEA
Sbjct: 61  FKHDISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRILNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAID I +RIKD QVA+++LAIKN GII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDLIIKRIKDFQVALVDLAIKNKGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLL+FVEQLDRDAGRLLDCR RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSI A+HLSRLGEEWVLWSSEEFGFI P+
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPS 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGDLVTLL+LCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLSLD+LR LSPIF++DVYEFLGVENAVNKFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGKNCQLKDLSLDELRALSPIFNEDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

Query: 481 HWIEKLGISQ 491
           HWIEKLGISQ
Sbjct: 481 HWIEKLGISQ 486

BLAST of Moc08g02680 vs. NCBI nr
Match: KAG6598729.1 (Argininosuccinate lyase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 896.7 bits (2316), Expect = 8.7e-257
Identity = 452/490 (92.24%), Postives = 467/490 (95.31%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT  DVSSA EA G TSAA    +KK E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTIFDVSSAAEATGETSAA----AKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           +KHDISGSRAHASMLAKQGLMSISDRD ILAGLDEIE+RI NGEFVWRADREDVHMNIEA
Sbjct: 61  FKHDISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRILNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI ERIKD QVA+++LAIKN GII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIIERIKDFQVAMVDLAIKNKGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLL+FVEQLDRDAGRLLDCR RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSI A+HLSRLGEEWVLWSSEEFGFI P+
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPS 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGDLVTLL+LCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLSL +LR LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGKNCQLKDLSLAELRALSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

Query: 481 HWIEKLGISQ 491
           +WIEKLGISQ
Sbjct: 481 YWIEKLGISQ 486

BLAST of Moc08g02680 vs. ExPASy Swiss-Prot
Match: Q9LEU8 (Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10920 PE=2 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 7.2e-214
Identity = 350/461 (75.92%), Postives = 417/461 (90.46%), Query Frame = 0

Query: 29  TKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDISGSRAHASMLAKQGLMSISDRDS 88
           +KE+KLWGGRF+ESVT+ VE+FTESIS+DK LYK DI GS+AHASMLA QGL++ SD+DS
Sbjct: 55  SKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDS 114

Query: 89  ILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 148
           IL GLD+IER+I   +F WR DREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR
Sbjct: 115 ILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 174

Query: 149 LWCRDAIDSIAERIKDLQVAILELAIKNAGIIVPGYTHMQRAQPVLLQHLLLSFVEQLDR 208
           LWCRDAID+I  +I++LQ A++ELA+KN  +IVPGYTH+QRAQPVLL H+LL+FVEQL+R
Sbjct: 175 LWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLER 234

Query: 209 DAGRLLDCRVRLNFCPLGACALAGTGLPIDRFMTSSALGFSSPLRNSIDAVSDRDFAMEF 268
           DAGR +DCR RLNF PLGACALAGTGLPIDRFMT++ALGF+ P+RNSIDAVSDRDF +EF
Sbjct: 235 DAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEF 294

Query: 269 LSANSITAVHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGR 328
           L  N+ T +HLSRLGEEWVLW+SEEFGF+TP+D+VSTGSSIMPQKKNPDPMELVRGKS R
Sbjct: 295 LYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSAR 354

Query: 329 VIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKAVSGMLEVSAEFARNISFNQDRIKK 388
           VIGDLVT+LTLCKGLPLAYNRD QEDKEP+FDS K + GM++VSAEFA+N++FN+DRIKK
Sbjct: 355 VIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKK 414

Query: 389 ALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGRNCQLKDLSLDDLRTLSPIFD 448
           +LPAGHLDATTLADYLV KG+PFR+SHDIVGK V +C+ + C+L++LSL++++ LSP+F+
Sbjct: 415 SLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFE 474

Query: 449 DDVYEFLGVENAVNKFSSYGSTGSECVASQLEHWIEKLGIS 490
           +DV+ FLGVEN+VNKFSSYGSTGS CVA QL +W+ KL I+
Sbjct: 475 EDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNIT 515

BLAST of Moc08g02680 vs. ExPASy Swiss-Prot
Match: Q6AR60 (Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) OX=177439 GN=argH PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 7.7e-131
Identity = 229/444 (51.58%), Postives = 305/444 (68.69%), Query Frame = 0

Query: 28  ETKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDISGSRAHASMLAKQGLMSISDRD 87
           E K  KLWGGRF E++  +VE+F+ESISYD  LYK+DI+GS+AHA+ML+ QG++S  + +
Sbjct: 3   EQKSKKLWGGRFSEAMAASVEKFSESISYDVRLYKYDIAGSKAHATMLSSQGIISPEELE 62

Query: 88  SILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDF 147
            I+AGL  IE  I  G F ++ + EDVHMNIE AL D IG    +LH ARSRNDQ+A DF
Sbjct: 63  QIIAGLSSIEADIEAGVFEFKTEYEDVHMNIEQALVDRIGAAGSRLHAARSRNDQIALDF 122

Query: 148 RLWCRDAIDSIAERIKDLQVAILELAIKNAGIIVPGYTHMQRAQPVLLQHLLLSFVEQLD 207
           +++ RD  D + E +     A   +  K  G I+PGYTH QRAQPVL+ H +L++ E   
Sbjct: 123 KMYLRDQCDHLVELLDGACRAFTVVGRKYLGDIMPGYTHTQRAQPVLITHHMLAYYEMFR 182

Query: 208 RDAGRLLDCRVRLNFCPLGACALAGTGLPIDRFMTSSALGFSSPLRNSIDAVSDRDFAME 267
           RD  R+LDCR RLN  PLG  A+AGTGLPI+R   + ALGF+    NS+D  +DRD+A+E
Sbjct: 183 RDRDRILDCRKRLNLSPLGCAAMAGTGLPINREQVAKALGFAGVTANSMDTSADRDYAIE 242

Query: 268 FLSANSITAVHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSG 327
             S  ++  +HLSRL EE V WS+ E+ F+  +D+  TGSSIMPQKKNPD  EL+RGKSG
Sbjct: 243 LTSCLTMIQLHLSRLAEELVTWSTSEYKFVDISDSFCTGSSIMPQKKNPDIAELIRGKSG 302

Query: 328 RVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKAVSGMLEVSAEFARNISFNQDRIK 387
           RV+G L++L+T+ KGLPL YNRD QEDKEPVFD++  VS  L ++AE   ++ FN  R  
Sbjct: 303 RVVGSLISLITMMKGLPLTYNRDQQEDKEPVFDAIDTVSASLSITAEMMAHMKFNTARCA 362

Query: 388 KALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGRNCQLKDLSLDDLRTLSPIF 447
           +A   G + AT LADYLV K +PFR +H IVG +VA C+ + C+L DL+L +++  SP+ 
Sbjct: 363 EATETGFITATDLADYLVMKDVPFRQAHHIVGSAVAACIAKGCELPDLTLTEMQEFSPVI 422

Query: 448 DDDVYEFLGVENAVNKFSSYGSTG 472
           + DV+  L  E +VN   S G TG
Sbjct: 423 ESDVFAVLTAEGSVNSRVSTGGTG 446

BLAST of Moc08g02680 vs. ExPASy Swiss-Prot
Match: Q2LT96 (Argininosuccinate lyase OS=Syntrophus aciditrophicus (strain SB) OX=56780 GN=argH PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 7.2e-129
Identity = 237/454 (52.20%), Postives = 307/454 (67.62%), Query Frame = 0

Query: 26  KAETKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDISGSRAHASMLAKQGLMSISD 85
           K  T++ K WGGRFQE   + VE FT SIS+D+ LY++DI GS AHA ML +Q +++  +
Sbjct: 2   KDSTEKSKPWGGRFQEPTNELVEAFTASISFDRRLYRYDIEGSIAHARMLVRQEIINRKE 61

Query: 86  RDSILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVAT 145
             +I+ GL EI+R I  G F +    ED+HM IE  L   IG+   KLHT RSRNDQVA 
Sbjct: 62  GFAIVEGLKEIQRDIETGSFEFSPGDEDIHMAIEKDLIRRIGDAGAKLHTGRSRNDQVAL 121

Query: 146 DFRLWCRDAIDSIAERIKDLQVAILELAIKNAGIIVPGYTHMQRAQPVLLQHLLLSFVEQ 205
           D RL+ R  I  I E +K L+ A LELA K A  I+PGYTH+Q+AQPVLL H LL++ E 
Sbjct: 122 DVRLYLRAEIKEILEFMKVLKGAFLELAKKEAETILPGYTHLQKAQPVLLAHYLLAWREM 181

Query: 206 LDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRFMTSSALGFSSPLRNSIDAVSDRDFA 265
           LDRD  RL DC  R+N  PLGA ALAGT LPIDR   +  L F    RNS+D VSDRDF 
Sbjct: 182 LDRDESRLRDCYRRVNVLPLGAAALAGTSLPIDRAYVARLLKFPEISRNSMDTVSDRDFV 241

Query: 266 MEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGK 325
            EFL A+S+  +HLSR  E+ +LWSS EF F+  +DA +TGSSIMPQKKNPD  EL+RGK
Sbjct: 242 AEFLFASSLIMMHLSRFCEDLILWSSGEFNFVEISDAFTTGSSIMPQKKNPDVAELIRGK 301

Query: 326 SGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKAVSGMLEVSAEFARNISFNQDR 385
           +GRV G+L+ LLTL KGLP+ YNRDLQEDKEP+FD+V  V   L++ AE  RN+ FN+++
Sbjct: 302 TGRVYGNLIALLTLLKGLPMTYNRDLQEDKEPLFDTVDTVKACLQILAEMIRNMKFNREK 361

Query: 386 IKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGRNCQLKDLSLDDLRTLSP 445
           +++    G   AT LA+YLV KGIPFR +H IVGK V+ C+    +L  LS+++ +   P
Sbjct: 362 MRQEAEGGFSTATDLAEYLVMKGIPFREAHGIVGKLVSFCIECKKELAQLSMEEFQRFCP 421

Query: 446 IFDDDVYEFLGVENAVNKFSSYGSTGSECVASQL 480
           + ++D+++ L V N+V    SYG T    V  Q+
Sbjct: 422 VINEDIHDRLSVSNSVRSRKSYGGTACRQVMEQI 455

BLAST of Moc08g02680 vs. ExPASy Swiss-Prot
Match: B7KW75 (Argininosuccinate lyase OS=Methylorubrum extorquens (strain CM4 / NCIMB 13688) OX=440085 GN=argH PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.6e-128
Identity = 229/456 (50.22%), Postives = 310/456 (67.98%), Query Frame = 0

Query: 33  KLWGGRFQESVTDAVERFTESISYDKDLYKHDISGSRAHASMLAKQGLMSISDRDSILAG 92
           ++WGGRF     + +E    SI +D+ L   DI GS AH +ML  QG++   D  +I AG
Sbjct: 4   RMWGGRFASGPAEIMEEINASIGFDRRLASQDIRGSLAHVAMLGSQGILPAEDVAAIEAG 63

Query: 93  LDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCR 152
           L  +E  I  GEFV+R + ED+HM +E+ LT+++G  A +LHTARSRNDQVATD RLW R
Sbjct: 64  LKSVEAEIARGEFVFRRELEDIHMAVESRLTEIVGPAAGRLHTARSRNDQVATDMRLWVR 123

Query: 153 DAIDSIAERIKDLQVAILELAIKNAGIIVPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGR 212
           D +D++  ++ DLQ A+ E A+K+AG ++PG+TH+Q AQPV   H  L++VE L RD GR
Sbjct: 124 DTLDALDAQVADLQRALAETAVKHAGTVMPGFTHLQSAQPVTFGHHCLAYVEMLARDRGR 183

Query: 213 LLDCRVRLNFCPLGACALAGTGLPIDRFMTSSALGFSSPLRNSIDAVSDRDFAMEFLSAN 272
             D R RLN CPLGA ALAGT  PIDR  T++ALGF  P  NS+D+V+DRDFA+E LSA 
Sbjct: 184 FRDARARLNECPLGAAALAGTSFPIDRHATAAALGFDRPTANSLDSVADRDFALESLSAA 243

Query: 273 SITAVHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGRVIGD 332
           SI AVHLSR  EE V+W+S +FGF+  +D  +TGSSIMPQK+NPD  ELVR K+GR+IG 
Sbjct: 244 SICAVHLSRFAEELVVWTSAQFGFVRLSDGFTTGSSIMPQKRNPDAAELVRAKAGRIIGA 303

Query: 333 LVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKAVSGMLEVSAEFARNISFNQDRIKKALPA 392
           L  LL + KGLPLAY++D+QEDKE  FD+++++S  L   A   R++    + +K+A  +
Sbjct: 304 LTGLLIVMKGLPLAYSKDMQEDKEGTFDALQSLSLCLAAMAGMVRDLEPVAETLKRAAGS 363

Query: 393 GHLDATTLADYLVHK-GIPFRTSHDIVGKSVAMCLGRNCQLKDLSLDDLRTLSPIFDDDV 452
           G+  AT LAD+LV +  +PFR +H + G+ VA    R   L++LSL D++ +     D V
Sbjct: 364 GYATATDLADWLVRELNMPFRQAHHVTGRVVAAASERGIGLEELSLQDMQAVEAGITDAV 423

Query: 453 YEFLGVENAVNKFSSYGSTGSECVASQLEHWIEKLG 488
           +  LGVEN+V   +SYG T  + V  Q E W+EKLG
Sbjct: 424 FAVLGVENSVASRTSYGGTAPDNVRRQAEGWLEKLG 459

BLAST of Moc08g02680 vs. ExPASy Swiss-Prot
Match: Q3JDS2 (Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=argH PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.7e-128
Identity = 239/454 (52.64%), Postives = 305/454 (67.18%), Query Frame = 0

Query: 33  KLWGGRFQESVTDAVERFTESISYDKDLYKHDISGSRAHASMLAKQGLMSISDRDSILAG 92
           K W GRF E     VE FT SI +D  LY+ DI GS AHA MLAK G++S  + D+I+ G
Sbjct: 10  KPWSGRFHEPTDTFVEEFTASIGFDHRLYRQDIHGSIAHARMLAKVGVLSREECDAIIQG 69

Query: 93  LDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCR 152
           L+ I + I  G+F W    EDVHMNIEAALT+ IG   KKLHT RSRNDQ+ATD RL+ R
Sbjct: 70  LETIAQDISRGQFSWSVALEDVHMNIEAALTERIGPVGKKLHTGRSRNDQIATDIRLYLR 129

Query: 153 DAIDSIAERIKDLQVAILELAIKNAGIIVPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGR 212
           DAID I  +++ LQ  +L +A + A  I+PG+TH+Q AQPV   H L+++ E L RD GR
Sbjct: 130 DAIDLITAQLQRLQEGLLAIAEREAATIMPGFTHLQTAQPVTFGHHLMAWFEMLLRDTGR 189

Query: 213 LLDCRVRLNFCPLGACALAGTGLPIDRFMTSSALGFSSPLRNSIDAVSDRDFAMEFLSAN 272
           L DCR R+N  PLGA ALAGT  PIDR  T+  LGF     NS+DAVSDRDFA+EF +A 
Sbjct: 190 LADCRHRVNVLPLGAAALAGTTFPIDRAYTAELLGFDGIAENSLDAVSDRDFAIEFTAAG 249

Query: 273 SITAVHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGRVIGD 332
           ++   HLSR  EE VLWSS +F FIT  D   TGSSIMPQKKNPD  ELVRGK+GRV G 
Sbjct: 250 ALLMTHLSRFAEELVLWSSAQFDFITLPDRFCTGSSIMPQKKNPDVPELVRGKTGRVNGH 309

Query: 333 LVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKAVSGMLEVSAEFARNISFNQDRIKKALPA 392
           LV+LLTL KG PLAYN+D QEDKEP+FD+V  + G L   A+    I+ N D++++A   
Sbjct: 310 LVSLLTLMKGQPLAYNKDNQEDKEPLFDTVDTLLGCLRAFADMIPAITTNPDKMREAATK 369

Query: 393 GHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVY 452
           G+  AT LADYLV KG+ FR +H+IVGK+VA+ + ++  L  L L  L+  SP+ ++DV+
Sbjct: 370 GYATATDLADYLVRKGVSFRDAHEIVGKAVALAIEQSKDLAALKLATLQDFSPVIEEDVF 429

Query: 453 EFLGVENAVNKFSSYGSTGSECVASQLEHWIEKL 487
           + L +E +V   +  G T    V + ++   EKL
Sbjct: 430 DVLTLEGSVAARNHLGGTAPAQVRAAIQRGREKL 463

BLAST of Moc08g02680 vs. ExPASy TrEMBL
Match: A0A6J1BUL3 (argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111004891 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.1e-278
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

Query: 481 HWIEKLGISQ 491
           HWIEKLGISQ
Sbjct: 481 HWIEKLGISQ 490

BLAST of Moc08g02680 vs. ExPASy TrEMBL
Match: A0A6J1K8P1 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492127 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.2e-257
Identity = 451/490 (92.04%), Postives = 467/490 (95.31%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT  DVSSA EA G TSAA    +KK E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTIFDVSSAAEATGETSAA----AKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           +KHDISGSRAHASMLAKQGLMSISDRD ILAGLDEIE+RI NGEFVWRADREDVHMNIEA
Sbjct: 61  FKHDISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRILNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAID I +RIKD QVA+++LAIKN GII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDLIIKRIKDFQVALVDLAIKNKGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLL+FVEQLDRDAGRLLDCR RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSI A+HLSRLGEEWVLWSSEEFGFI P+
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPS 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGDLVTLL+LCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLSLD+LR LSPIF++DVYEFLGVENAVNKFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGKNCQLKDLSLDELRALSPIFNEDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

Query: 481 HWIEKLGISQ 491
           HWIEKLGISQ
Sbjct: 481 HWIEKLGISQ 486

BLAST of Moc08g02680 vs. ExPASy TrEMBL
Match: A0A6J1HD89 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462454 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 5.5e-257
Identity = 451/490 (92.04%), Postives = 467/490 (95.31%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT  DVSSA EA G TSAA    +KK E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTIFDVSSAAEATGETSAA----AKKPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           +KHDISGSRAHASMLAKQGLMSISDRD ILAGLDEIE+RI NGEFVWRADREDVHMNIEA
Sbjct: 61  FKHDISGSRAHASMLAKQGLMSISDRDCILAGLDEIEKRILNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI ERIKD QVA+++LAIKN GII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIIERIKDFQVAMVDLAIKNKGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLL+F+EQLDRDAGRLLDCR RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFIEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSI A+HLSRLGEEWVLWSSEEFGFI P+
Sbjct: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSIMAIHLSRLGEEWVLWSSEEFGFIVPS 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGDLVTLL+LCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTLLSLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK VSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLSL +LR LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQLE
Sbjct: 421 SVAMCLGKNCQLKDLSLAELRALSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480

Query: 481 HWIEKLGISQ 491
           +WIEKLGISQ
Sbjct: 481 YWIEKLGISQ 486

BLAST of Moc08g02680 vs. ExPASy TrEMBL
Match: A0A5A7VGX5 (Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004280 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.1e-256
Identity = 445/488 (91.19%), Postives = 468/488 (95.90%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT+LDV SA +A GA+SAA    SK+ E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTKLDVQSASQATGASSAA----SKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDI GSRAHASMLAKQGLMSISDRDSILAGLDEIERRI+NGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDIRGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIQNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI ERIKD QVA+++LA+KNAGII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVERIKDFQVAMVDLAVKNAGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQHLLL+FVEQLDRDAGRLLDCR RLNFCPLGACALAGTGLPIDRF
Sbjct: 181 VPGYTHLQRAQPVLLQHLLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIDRF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSAL FSSPLRNSIDAVSDRDFA+EFLSANS+ A+HLSRLGEEWVLW SEEFGFITPN
Sbjct: 241 MTSSALEFSSPLRNSIDAVSDRDFAIEFLSANSVMAIHLSRLGEEWVLWCSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK V GMLEVS EFARNISFNQDRIKKALPAGHLDATTLADYLVHKG+PFRTSHDIVGK
Sbjct: 361 SVKTVLGMLEVSTEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGMPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLS+D+LRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQL+
Sbjct: 421 SVAMCLGKNCQLKDLSIDELRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLQ 480

Query: 481 HWIEKLGI 489
           +W+EKLG+
Sbjct: 481 YWMEKLGL 484

BLAST of Moc08g02680 vs. ExPASy TrEMBL
Match: A0A0A0LPM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370510 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 6.1e-256
Identity = 446/488 (91.39%), Postives = 468/488 (95.90%), Query Frame = 0

Query: 1   MTELDVSSAPEAAGATSAAGSSVSKKAETKEIKLWGGRFQESVTDAVERFTESISYDKDL 60
           MT+LDV SA +A GATSAA    SK+ E KEIKLWGGRFQESVTDAVERFTESISYDKDL
Sbjct: 1   MTKLDVQSASQATGATSAA----SKQPEAKEIKLWGGRFQESVTDAVERFTESISYDKDL 60

Query: 61  YKHDISGSRAHASMLAKQGLMSISDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120
           YKHDI GSRAHASMLAKQGLMSI DRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA
Sbjct: 61  YKHDIRGSRAHASMLAKQGLMSIGDRDSILAGLDEIERRIRNGEFVWRADREDVHMNIEA 120

Query: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIAERIKDLQVAILELAIKNAGII 180
           ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSI  RIKD QVA+++LA+KNAGII
Sbjct: 121 ALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDSIVARIKDFQVAMVDLAVKNAGII 180

Query: 181 VPGYTHMQRAQPVLLQHLLLSFVEQLDRDAGRLLDCRVRLNFCPLGACALAGTGLPIDRF 240
           VPGYTH+QRAQPVLLQH+LL+FVEQLDRDAGRLLDCR RLNFCPLGACALAGTGLPI+RF
Sbjct: 181 VPGYTHLQRAQPVLLQHVLLAFVEQLDRDAGRLLDCRARLNFCPLGACALAGTGLPIERF 240

Query: 241 MTSSALGFSSPLRNSIDAVSDRDFAMEFLSANSITAVHLSRLGEEWVLWSSEEFGFITPN 300
           MTSSAL FSSPLRNSIDAVSDRDFA+EFLSANSI A+HLSRLGEEWVLWSSEEFGFITPN
Sbjct: 241 MTSSALEFSSPLRNSIDAVSDRDFAIEFLSANSIMAIHLSRLGEEWVLWSSEEFGFITPN 300

Query: 301 DAVSTGSSIMPQKKNPDPMELVRGKSGRVIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFD 360
           DAVSTGSSIMPQKKNPDPMELVRGKS RVIGD+VTLLTLCKGLPLAYNRDLQEDKEPVFD
Sbjct: 301 DAVSTGSSIMPQKKNPDPMELVRGKSARVIGDVVTLLTLCKGLPLAYNRDLQEDKEPVFD 360

Query: 361 SVKAVSGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420
           SVK V GMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK
Sbjct: 361 SVKTVLGMLEVSAEFARNISFNQDRIKKALPAGHLDATTLADYLVHKGIPFRTSHDIVGK 420

Query: 421 SVAMCLGRNCQLKDLSLDDLRTLSPIFDDDVYEFLGVENAVNKFSSYGSTGSECVASQLE 480
           SVAMCLG+NCQLKDLSL++LR+LSPIFD+DVYEFLGVENAVNKFSSYGSTGSECVASQL+
Sbjct: 421 SVAMCLGKNCQLKDLSLNELRSLSPIFDEDVYEFLGVENAVNKFSSYGSTGSECVASQLQ 480

Query: 481 HWIEKLGI 489
           +WIEKLG+
Sbjct: 481 YWIEKLGL 484

BLAST of Moc08g02680 vs. TAIR 10
Match: AT5G10920.1 (L-Aspartase-like family protein )

HSP 1 Score: 744.6 bits (1921), Expect = 5.1e-215
Identity = 350/461 (75.92%), Postives = 417/461 (90.46%), Query Frame = 0

Query: 29  TKEIKLWGGRFQESVTDAVERFTESISYDKDLYKHDISGSRAHASMLAKQGLMSISDRDS 88
           +KE+KLWGGRF+ESVT+ VE+FTESIS+DK LYK DI GS+AHASMLA QGL++ SD+DS
Sbjct: 55  SKEVKLWGGRFEESVTEKVEKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDS 114

Query: 89  ILAGLDEIERRIRNGEFVWRADREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 148
           IL GLD+IER+I   +F WR DREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR
Sbjct: 115 ILRGLDDIERQIEANKFEWRTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFR 174

Query: 149 LWCRDAIDSIAERIKDLQVAILELAIKNAGIIVPGYTHMQRAQPVLLQHLLLSFVEQLDR 208
           LWCRDAID+I  +I++LQ A++ELA+KN  +IVPGYTH+QRAQPVLL H+LL+FVEQL+R
Sbjct: 175 LWCRDAIDTIIVKIRNLQRALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLER 234

Query: 209 DAGRLLDCRVRLNFCPLGACALAGTGLPIDRFMTSSALGFSSPLRNSIDAVSDRDFAMEF 268
           DAGR +DCR RLNF PLGACALAGTGLPIDRFMT++ALGF+ P+RNSIDAVSDRDF +EF
Sbjct: 235 DAGRYVDCRARLNFSPLGACALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEF 294

Query: 269 LSANSITAVHLSRLGEEWVLWSSEEFGFITPNDAVSTGSSIMPQKKNPDPMELVRGKSGR 328
           L  N+ T +HLSRLGEEWVLW+SEEFGF+TP+D+VSTGSSIMPQKKNPDPMELVRGKS R
Sbjct: 295 LYTNANTGIHLSRLGEEWVLWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSAR 354

Query: 329 VIGDLVTLLTLCKGLPLAYNRDLQEDKEPVFDSVKAVSGMLEVSAEFARNISFNQDRIKK 388
           VIGDLVT+LTLCKGLPLAYNRD QEDKEP+FDS K + GM++VSAEFA+N++FN+DRIKK
Sbjct: 355 VIGDLVTVLTLCKGLPLAYNRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKK 414

Query: 389 ALPAGHLDATTLADYLVHKGIPFRTSHDIVGKSVAMCLGRNCQLKDLSLDDLRTLSPIFD 448
           +LPAGHLDATTLADYLV KG+PFR+SHDIVGK V +C+ + C+L++LSL++++ LSP+F+
Sbjct: 415 SLPAGHLDATTLADYLVKKGMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFE 474

Query: 449 DDVYEFLGVENAVNKFSSYGSTGSECVASQLEHWIEKLGIS 490
           +DV+ FLGVEN+VNKFSSYGSTGS CVA QL +W+ KL I+
Sbjct: 475 EDVFGFLGVENSVNKFSSYGSTGSNCVAEQLGYWVNKLNIT 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131833.12.4e-278100.00argininosuccinate lyase, chloroplastic-like [Momordica charantia][more]
XP_038886091.15.8e-26193.24argininosuccinate lyase, chloroplastic-like [Benincasa hispida][more]
XP_023546913.11.3e-25792.45argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022997120.18.7e-25792.04argininosuccinate lyase, chloroplastic-like [Cucurbita maxima][more]
KAG6598729.18.7e-25792.24Argininosuccinate lyase, chloroplastic, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
Q9LEU87.2e-21475.92Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10... [more]
Q6AR607.7e-13151.58Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) ... [more]
Q2LT967.2e-12952.20Argininosuccinate lyase OS=Syntrophus aciditrophicus (strain SB) OX=56780 GN=arg... [more]
B7KW751.6e-12850.22Argininosuccinate lyase OS=Methylorubrum extorquens (strain CM4 / NCIMB 13688) O... [more]
Q3JDS22.7e-12852.64Argininosuccinate lyase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BUL31.1e-278100.00argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1K8P14.2e-25792.04argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HD895.5e-25792.04argininosuccinate lyase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A5A7VGX52.1e-25691.19Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A0A0LPM36.1e-25691.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370510 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G10920.15.1e-21575.92L-Aspartase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 178..196
score: 39.74
coord: 306..322
score: 64.09
coord: 262..289
score: 27.97
coord: 133..151
score: 38.14
NoneNo IPR availablePRINTSPR00145ARGSUCLYASEcoord: 306..322
score: 81.13
coord: 224..240
score: 67.02
coord: 132..154
score: 72.51
coord: 173..193
score: 55.02
coord: 262..286
score: 52.92
coord: 341..360
score: 76.96
NoneNo IPR availableGENE3D1.10.40.30coord: 391..463
e-value: 1.6E-184
score: 615.7
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 150..479
e-value: 1.6E-184
score: 615.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR43814:SF4ARGININOSUCCINATE LYASEcoord: 23..489
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 37..331
e-value: 4.4E-67
score: 226.7
IPR029419Argininosuccinate lyase, C-terminalPFAMPF14698ASL_C2coord: 396..462
e-value: 2.2E-21
score: 76.4
IPR009049Argininosuccinate lyaseTIGRFAMTIGR00838TIGR00838coord: 34..483
e-value: 6.9E-173
score: 573.8
IPR009049Argininosuccinate lyasePANTHERPTHR43814ARGININOSUCCINATE LYASEcoord: 23..489
IPR009049Argininosuccinate lyaseHAMAPMF_00006Arg_succ_lyasecoord: 33..484
score: 40.097672
IPR009049Argininosuccinate lyaseCDDcd01359Argininosuccinate_lyasecoord: 53..486
e-value: 0.0
score: 656.157
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 53..260
e-value: 1.6E-184
score: 615.7
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 306..315
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 37..486

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g02680.1Moc08g02680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042450 arginine biosynthetic process via ornithine
cellular_component GO:0005829 cytosol
molecular_function GO:0004056 argininosuccinate lyase activity
molecular_function GO:0003824 catalytic activity