Moc08g00660 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g00660
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectate lyase
Locationchr8: 378460 .. 379961 (-)
RNA-Seq ExpressionMoc08g00660
SyntenyMoc08g00660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAATTAATCCCATCTTCGTCCTTCTGATAATCTTTGCCACTCTTCTTCTCCAAATGAGCTCCATTTCATCCTCTCCCGTGGCCGACCCCGAGACGATCGTTGAAGAAGTTCTCACGTAATTATTTAATTTCTTCTTTTTTCTTGATCTTTCTTGGGACTATTAATTAAAGTTGGGTTTGGGTTGAAAATAAAAACAAATATTGTCGTGGTTTGCAGGAGCATCAACAATGCGAGTAGAAGGAAGTTGGGGTTTCTGTCGTGTGGAACCGGGAACCCAATCGACGACTGCTGGCGGTGCGACCCCAACTGGGAGAAGAACCGGAAGCGGCTCGCGGACTGCGGCATTGGGTTCGGAAAGCGAGCCATTGGCGGCAAAGACGGAAAGTTTTACGTGGTGACTGACCCAAGCGACAAAGACGTGGTGAACCCGAAGCCGGGGACTCTACGCCACGCCGTGATCCAAAAGGAGCCGCTGTGGATAACCTTCAAGCGGGACATGGTGATAAAACTGAAGGCGGAGCTGTTGATGAACTCGTTCAAGACCATCGACGGGCGTGGGGCGTCGGTGCACATCGCGGGTGGGCCCTGCATAACCATACAATTCGTGACCAACATAATAATCCACGGCATCAACATCCACGACTGCAAGCAGGGGGGGAACACGTACGTCCGCGATTCGCCGGACCACTACGGCTGGAGAACTCTATCGGACGGCGACGGGGTTTCCATTTTTGGCGGCACCCATATTTGGATCGACCATTGTTCTCTGTCCAACTGCCGCGATGGGCTGATCGATGCCATTCATGGGTCCACTGCCATCACCATTTCTAACAATTACATGACCCACCACAACAAGGTCATGCTTTTGGGCCATAGCGATTCCTACACTCAAGACAAGAATATGCAAGTTACTATAGCTTTTAACCATTTTGGTGAAGGACTCGTACAGAGAATGCCCAGGTAACTGCACGATATCTATATATTAATTAAATTTACTAGTATTAAAATTTTGGGTTGATTTTGATTATGGGTGTTGTTTTTTGGCAGATGCCGACATGGGTATTTTCATGTGGTCAATAATGACTATACCCATTGGATAATGTACGCCATTGGTGGCAGTGCATCTCCCACCATCAACAGCCAAGGGAACCGTTACCTTGCTCCCAATGACCAAAACAACAAAGAGGTGAGAATTATAATTGGTAATAAATTTCCTTTTATTAAAAATTGAGTTTAATAGTAATTTAACATGTTATTTAATTAATTGCGGTGGCAGGTGACGAAGCATGAGGATGCACCGCAGAGCCAATGGAAGAATTGGAATTGGAGATCGAGTGGAGATTTAATGTTGAATGGTGCGTTCTTCACGATGTCGGGTGCTGGAGCATCGTCAAGCTACGCCAGGGCGTCGAGCTTGAGTGCGAAGTCGTCGTCTCTGGTGGGCTCCATGACGGCCACGGCCGGGGTGCTGAGATGTAGAAAAGGCTCTCGGTGCTAG

mRNA sequence

ATGGAAATTAATCCCATCTTCGTCCTTCTGATAATCTTTGCCACTCTTCTTCTCCAAATGAGCTCCATTTCATCCTCTCCCGTGGCCGACCCCGAGACGATCGTTGAAGAAGTTCTCACGAGCATCAACAATGCGAGTAGAAGGAAGTTGGGGTTTCTGTCGTGTGGAACCGGGAACCCAATCGACGACTGCTGGCGGTGCGACCCCAACTGGGAGAAGAACCGGAAGCGGCTCGCGGACTGCGGCATTGGGTTCGGAAAGCGAGCCATTGGCGGCAAAGACGGAAAGTTTTACGTGGTGACTGACCCAAGCGACAAAGACGTGGTGAACCCGAAGCCGGGGACTCTACGCCACGCCGTGATCCAAAAGGAGCCGCTGTGGATAACCTTCAAGCGGGACATGGTGATAAAACTGAAGGCGGAGCTGTTGATGAACTCGTTCAAGACCATCGACGGGCGTGGGGCGTCGGTGCACATCGCGGGTGGGCCCTGCATAACCATACAATTCGTGACCAACATAATAATCCACGGCATCAACATCCACGACTGCAAGCAGGGGGGGAACACGTACGTCCGCGATTCGCCGGACCACTACGGCTGGAGAACTCTATCGGACGGCGACGGGGTTTCCATTTTTGGCGGCACCCATATTTGGATCGACCATTGTTCTCTGTCCAACTGCCGCGATGGGCTGATCGATGCCATTCATGGGTCCACTGCCATCACCATTTCTAACAATTACATGACCCACCACAACAAGGTCATGCTTTTGGGCCATAGCGATTCCTACACTCAAGACAAGAATATGCAAGTTACTATAGCTTTTAACCATTTTGGTGAAGGACTCGTACAGAGAATGCCCAGATGCCGACATGGGTATTTTCATGTGGTCAATAATGACTATACCCATTGGATAATGTACGCCATTGGTGGCAGTGCATCTCCCACCATCAACAGCCAAGGGAACCGTTACCTTGCTCCCAATGACCAAAACAACAAAGAGGTGACGAAGCATGAGGATGCACCGCAGAGCCAATGGAAGAATTGGAATTGGAGATCGAGTGGAGATTTAATGTTGAATGGTGCGTTCTTCACGATGTCGGGTGCTGGAGCATCGTCAAGCTACGCCAGGGCGTCGAGCTTGAGTGCGAAGTCGTCGTCTCTGGTGGGCTCCATGACGGCCACGGCCGGGGTGCTGAGATGTAGAAAAGGCTCTCGGTGCTAG

Coding sequence (CDS)

ATGGAAATTAATCCCATCTTCGTCCTTCTGATAATCTTTGCCACTCTTCTTCTCCAAATGAGCTCCATTTCATCCTCTCCCGTGGCCGACCCCGAGACGATCGTTGAAGAAGTTCTCACGAGCATCAACAATGCGAGTAGAAGGAAGTTGGGGTTTCTGTCGTGTGGAACCGGGAACCCAATCGACGACTGCTGGCGGTGCGACCCCAACTGGGAGAAGAACCGGAAGCGGCTCGCGGACTGCGGCATTGGGTTCGGAAAGCGAGCCATTGGCGGCAAAGACGGAAAGTTTTACGTGGTGACTGACCCAAGCGACAAAGACGTGGTGAACCCGAAGCCGGGGACTCTACGCCACGCCGTGATCCAAAAGGAGCCGCTGTGGATAACCTTCAAGCGGGACATGGTGATAAAACTGAAGGCGGAGCTGTTGATGAACTCGTTCAAGACCATCGACGGGCGTGGGGCGTCGGTGCACATCGCGGGTGGGCCCTGCATAACCATACAATTCGTGACCAACATAATAATCCACGGCATCAACATCCACGACTGCAAGCAGGGGGGGAACACGTACGTCCGCGATTCGCCGGACCACTACGGCTGGAGAACTCTATCGGACGGCGACGGGGTTTCCATTTTTGGCGGCACCCATATTTGGATCGACCATTGTTCTCTGTCCAACTGCCGCGATGGGCTGATCGATGCCATTCATGGGTCCACTGCCATCACCATTTCTAACAATTACATGACCCACCACAACAAGGTCATGCTTTTGGGCCATAGCGATTCCTACACTCAAGACAAGAATATGCAAGTTACTATAGCTTTTAACCATTTTGGTGAAGGACTCGTACAGAGAATGCCCAGATGCCGACATGGGTATTTTCATGTGGTCAATAATGACTATACCCATTGGATAATGTACGCCATTGGTGGCAGTGCATCTCCCACCATCAACAGCCAAGGGAACCGTTACCTTGCTCCCAATGACCAAAACAACAAAGAGGTGACGAAGCATGAGGATGCACCGCAGAGCCAATGGAAGAATTGGAATTGGAGATCGAGTGGAGATTTAATGTTGAATGGTGCGTTCTTCACGATGTCGGGTGCTGGAGCATCGTCAAGCTACGCCAGGGCGTCGAGCTTGAGTGCGAAGTCGTCGTCTCTGGTGGGCTCCATGACGGCCACGGCCGGGGTGCTGAGATGTAGAAAAGGCTCTCGGTGCTAG

Protein sequence

MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNPIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC
Homology
BLAST of Moc08g00660 vs. NCBI nr
Match: XP_022131699.1 (probable pectate lyase 5 [Momordica charantia])

HSP 1 Score: 837.0 bits (2161), Expect = 6.8e-239
Identity = 407/407 (100.00%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNP 60
           MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNP
Sbjct: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           ITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLN 360

Query: 361 GAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           GAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC
Sbjct: 361 GAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 407

BLAST of Moc08g00660 vs. NCBI nr
Match: XP_022996659.1 (probable pectate lyase 5 [Cucurbita maxima])

HSP 1 Score: 769.2 bits (1985), Expect = 1.7e-218
Identity = 375/408 (91.91%), Postives = 393/408 (96.32%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGN 60
           MEI P F+ +I F TLLLQM SISSSPVADPE +V+EVL+SINNA +RR LGFLSCGTGN
Sbjct: 1   MEI-PSFLPIITF-TLLLQMCSISSSPVADPELVVQEVLSSINNATTRRNLGFLSCGTGN 60

Query: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHA 120
           PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGK+GKFYVVTDPSDKDVVNPKPGTLRHA
Sbjct: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHA 120

Query: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGIN 180
           VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQ+VTNIIIHGIN
Sbjct: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGIN 180

Query: 181 IHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGST 240
           IHDCKQGGNTYVRDSP+HYGWRTLSDGDGVSIFGG+H+WIDHCSLSNCRDGLIDAIHGST
Sbjct: 181 IHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGST 240

Query: 241 AITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300
           AIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 241 AITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300

Query: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLML 360
           DYTHWIMYAIGGSASPTINSQGNRYLAPN+++ KEVTK EDAPQS+WKNWNWRSSGDLML
Sbjct: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLML 360

Query: 361 NGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           NGAFFT SGAG+SSSYARASSLSAKSSSLV +MTA AGVLRCRKGSRC
Sbjct: 361 NGAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of Moc08g00660 vs. NCBI nr
Match: KAG7029228.1 (putative pectate lyase 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 768.1 bits (1982), Expect = 3.9e-218
Identity = 374/408 (91.67%), Postives = 393/408 (96.32%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGN 60
           MEI P F+ +I F TLLLQM +ISSSPVADPE +V+EVL+SINNA +RR LGFLSCGTGN
Sbjct: 1   MEI-PSFLPIITF-TLLLQMCAISSSPVADPELVVQEVLSSINNATTRRNLGFLSCGTGN 60

Query: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHA 120
           PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGK+GKFYVVTDPSDKDVVNPKPGTLRHA
Sbjct: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHA 120

Query: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGIN 180
           VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQ+VTNIIIHGIN
Sbjct: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGIN 180

Query: 181 IHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGST 240
           IHDCKQGGNTYVRDSP+HYGWRTLSDGDGVSIFGG+H+WIDHCSLSNCRDGLIDAIHGST
Sbjct: 181 IHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGST 240

Query: 241 AITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300
           AIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 241 AITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300

Query: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLML 360
           DYTHWIMYAIGGSASPTINSQGNRYLAPN+++ KEVTK EDAPQS+WKNWNWRSSGDLML
Sbjct: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLML 360

Query: 361 NGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           NGAFFT SGAG+SSSYARASSLSAKSSSLV +MTA AGVLRCRKGSRC
Sbjct: 361 NGAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of Moc08g00660 vs. NCBI nr
Match: XP_023545498.1 (probable pectate lyase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.9 bits (1979), Expect = 8.6e-218
Identity = 373/408 (91.42%), Postives = 393/408 (96.32%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGN 60
           MEI P F+ +I F TLLLQM +ISSSPV+DPE +V+EVL+SINNA +RR LGFLSCGTGN
Sbjct: 1   MEI-PSFLPIITF-TLLLQMCAISSSPVSDPELVVQEVLSSINNATTRRNLGFLSCGTGN 60

Query: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHA 120
           PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGK+GKFYVVTDPSDKDVVNPKPGTLRHA
Sbjct: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHA 120

Query: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGIN 180
           VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQ+VTNIIIHGIN
Sbjct: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGIN 180

Query: 181 IHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGST 240
           IHDCKQGGNTYVRDSP+HYGWRTLSDGDGVSIFGG+H+WIDHCSLSNCRDGLIDAIHGST
Sbjct: 181 IHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGST 240

Query: 241 AITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300
           AIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 241 AITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300

Query: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLML 360
           DYTHWIMYAIGGSASPTINSQGNRYLAPN+++ KEVTK EDAPQS+WKNWNWRSSGDLML
Sbjct: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLML 360

Query: 361 NGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           NGAFFT SGAG+SSSYARASSLSAKSSSLV +MTA AGVLRCRKGSRC
Sbjct: 361 NGAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of Moc08g00660 vs. NCBI nr
Match: XP_022962436.1 (probable pectate lyase 5 [Cucurbita moschata])

HSP 1 Score: 766.9 bits (1979), Expect = 8.6e-218
Identity = 373/408 (91.42%), Postives = 393/408 (96.32%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGN 60
           MEI P F+ +I F TLLLQM +ISSSPVADP+ +V+EVL+SINNA +RR LGFLSCGTGN
Sbjct: 1   MEI-PSFLPIITF-TLLLQMCAISSSPVADPQLVVQEVLSSINNATTRRNLGFLSCGTGN 60

Query: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHA 120
           PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGK+GKFYVVTDPSDKDVVNPKPGTLRHA
Sbjct: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHA 120

Query: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGIN 180
           VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQ+VTNIIIHGIN
Sbjct: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGIN 180

Query: 181 IHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGST 240
           IHDCKQGGNTYVRDSP+HYGWRTLSDGDGVSIFGG+H+WIDHCSLSNCRDGLIDAIHGST
Sbjct: 181 IHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGST 240

Query: 241 AITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300
           AIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 241 AITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300

Query: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLML 360
           DYTHWIMYAIGGSASPTINSQGNRYLAPN+++ KEVTK EDAPQS+WKNWNWRSSGDLML
Sbjct: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLML 360

Query: 361 NGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           NGAFFT SGAG+SSSYARASSLSAKSSSLV +MTA AGVLRCRKGSRC
Sbjct: 361 NGAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of Moc08g00660 vs. ExPASy Swiss-Prot
Match: Q9FXD8 (Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2)

HSP 1 Score: 680.2 bits (1754), Expect = 1.4e-194
Identity = 327/409 (79.95%), Postives = 361/409 (88.26%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSS--ISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTG 60
           M +  + + L +F+ LLL +S   I+S+PV++PE +V+EV   I NASRR LG LSCGTG
Sbjct: 1   MRMTLVHLSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKI-NASRRNLGVLSCGTG 60

Query: 61  NPIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRH 120
           NPIDDCWRCDP WEKNR+RLADC IGFGK AIGG+DGK YVVTD SDKDVVNPKPGTLRH
Sbjct: 61  NPIDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRH 120

Query: 121 AVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGI 180
           AVIQ EPLWI F RDMVIKLK EL+MNSFKTIDGRGASVHIAGG CIT+Q+VTNIIIHG+
Sbjct: 121 AVIQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGV 180

Query: 181 NIHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGS 240
           NIHDCK+ GN YVRDSP HYGWRT SDGD VSIFGG+H+W+DHCSLSNC DGLIDAIHGS
Sbjct: 181 NIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGS 240

Query: 241 TAITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 300
           TAITISNNY++HHNKVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN
Sbjct: 241 TAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 300

Query: 301 NDYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLM 360
           NDYTHW MYAIGGSA+PTINSQGNR+LAPND   KEVTK+EDAP+S+WK WNWRS GDL 
Sbjct: 301 NDYTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLF 360

Query: 361 LNGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           LNGAFFT SG GASSSYA+ASSLSA+ SSLV S+T+ AG L CRKGSRC
Sbjct: 361 LNGAFFTPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of Moc08g00660 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.0e-189
Identity = 311/400 (77.75%), Postives = 356/400 (89.00%), Query Frame = 0

Query: 11  IIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGNPIDDCWRCDP 70
           +IF   +L  +  SS PV+DPE +VEEV   IN + SRRKLGF SCG+GNPIDDCWRCD 
Sbjct: 33  LIFFCCILFSALASSLPVSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDDCWRCDK 92

Query: 71  NWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLWIT 130
           +WEKNRKRLADCGIGFGK AIGG+DG+ YVVTDP + D VNP+PGTLR+AVIQ EPLWI 
Sbjct: 93  DWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWII 152

Query: 131 FKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNT 190
           FKRDM I+LK EL+MNSFKT+DGRGASVHI+GGPCITIQ+VTNIIIHG++IHDCKQGGNT
Sbjct: 153 FKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNT 212

Query: 191 YVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAITISNNYMT 250
           YVRDSP+HYG+RT+SDGDGVSIFGG+H+W+DHCSLSNC DGLIDAI GSTAITISNNY+T
Sbjct: 213 YVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLT 272

Query: 251 HHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAI 310
           HHNKVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAI
Sbjct: 273 HHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAI 332

Query: 311 GGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGAFFTMSGA 370
           GGSA+PTINSQGNR+LAP+D ++KEVTKHEDAP+ +W+NWNWRS GDL+LNGAFFT SGA
Sbjct: 333 GGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGA 392

Query: 371 G--ASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           G   SSSY++ASSL+A+ SS VG +T  +G L C++GS C
Sbjct: 393 GPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Moc08g00660 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 656.4 bits (1692), Expect = 2.2e-187
Identity = 311/409 (76.04%), Postives = 357/409 (87.29%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINN--ASRRKLGFLSCGTG 60
           M+   +F+ ++ F   LL      SSPV DPE++VEEV  SIN   A RRKLG+LSC TG
Sbjct: 3   MQTKKLFITIVSF---LLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTG 62

Query: 61  NPIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRH 120
           NPIDDCWRCDP+WE++R+RLADC IGFGK AIGG+DG+ YVVTD  + + V+PKPGTLRH
Sbjct: 63  NPIDDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRH 122

Query: 121 AVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGI 180
           AV+Q EPLWI F+RDM I+LK EL+MNSFKTIDGRGASVHI+GGPCITIQ+VTNIIIHGI
Sbjct: 123 AVVQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGI 182

Query: 181 NIHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGS 240
           +IHDCKQGGN  VR SP H+GWRT+SDGDGVSIFGG+H+W+DHCS SNC DGLIDAI GS
Sbjct: 183 HIHDCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGS 242

Query: 241 TAITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 300
           TAIT+SNN+MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN
Sbjct: 243 TAITLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 302

Query: 301 NDYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLM 360
           NDYTHW MYAIGGSA+PTINSQGNR+LAPN + +KEVTKHEDAP+S+WK WNWRSSGDL+
Sbjct: 303 NDYTHWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLL 362

Query: 361 LNGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           LNGAFFT SG  ASSSYA+ASSL AK SSLVG +T+T+G L CRKGSRC
Sbjct: 363 LNGAFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Moc08g00660 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 621.7 bits (1602), Expect = 5.9e-177
Identity = 295/401 (73.57%), Postives = 336/401 (83.79%), Query Frame = 0

Query: 7   FVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNPIDDCWR 66
           F+LL+     L   SS+    V DP  +V++V  S+ NASRR L +LSC TGNPIDDCWR
Sbjct: 13  FLLLLTIGNTLAFSSSLPDH-VQDPNLVVDDVNRSVFNASRRSLAYLSCRTGNPIDDCWR 72

Query: 67  CDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPL 126
           CDPNWE NR+RLADC IGFGK AIGG+ G+ YVVTDP++ D VNP+PGTLR+AV Q+EPL
Sbjct: 73  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 132

Query: 127 WITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHDCKQG 186
           WI FKRDMVI+LK EL++ SFKTIDGRG+SVHI  GPC+ I + TNIIIHGINIHDCK G
Sbjct: 133 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 192

Query: 187 GNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAITISNN 246
               ++D P H GW   SDGD V+IFGG H+WIDHCSLSNC DGLIDAIHGSTAITISNN
Sbjct: 193 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 252

Query: 247 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 306
           +MTHH+KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 253 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 312

Query: 307 YAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGAFFTM 366
           YAIGGSASPTI SQGNR+LAPN + NKEVTKHEDAP+S+W++WNWRS GD++LNGA+F  
Sbjct: 313 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 372

Query: 367 SGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           SGA A S+YARASSLSA+ SSLVGS+T TAG L CR+G RC
Sbjct: 373 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of Moc08g00660 vs. ExPASy Swiss-Prot
Match: O24554 (Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 1.3e-176
Identity = 301/395 (76.20%), Postives = 335/395 (84.81%), Query Frame = 0

Query: 16  LLLQMSSI---SSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNPIDDCWRCDPNWE 75
           L+L +SS+   SSSP   P  IV EV  SI NASRR LG+LSCGTGNPIDDCWRCDPNW 
Sbjct: 8   LILFISSLAIASSSPSRTPHAIVNEVHKSI-NASRRNLGYLSCGTGNPIDDCWRCDPNWA 67

Query: 76  KNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLWITFKR 135
            NR+RLADC IGFGK A+GG++G+ YVVTDP + D VNP PGTLR+AVIQ EPLWI FKR
Sbjct: 68  NNRQRLADCAIGFGKNAMGGRNGRIYVVTDPGNDDPVNPVPGTLRYAVIQDEPLWIIFKR 127

Query: 136 DMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNTYVR 195
           DMVI+L+ EL+MNS KTIDGRG +VHI  GPCITI + +NIIIHGI+IHDCKQ GN  +R
Sbjct: 128 DMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIIIHGIHIHDCKQAGNGNIR 187

Query: 196 DSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAITISNNYMTHHN 255
           +SP H GW T SDGDG+SIF    IWIDH SLSNC DGLIDAIHGSTAITISNNYMTHH+
Sbjct: 188 NSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAIHGSTAITISNNYMTHHD 247

Query: 256 KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAIGGS 315
           KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAIGGS
Sbjct: 248 KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGS 307

Query: 316 ASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGAFFTMSGAGAS 375
           ASPTI SQGNR+LAPN + +KEVTKHE+AP+S+WKNWNWRS GDLMLNGA+F  SG  A+
Sbjct: 308 ASPTIYSQGNRFLAPNTRFDKEVTKHENAPESEWKNWNWRSEGDLMLNGAYFRESGGRAA 367

Query: 376 SSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           SS+ARASSLS + S+LV SMT +AG L CRKGSRC
Sbjct: 368 SSFARASSLSGRPSTLVASMTRSAGALVCRKGSRC 401

BLAST of Moc08g00660 vs. ExPASy TrEMBL
Match: A0A6J1BR07 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111004807 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.3e-239
Identity = 407/407 (100.00%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNP 60
           MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNP
Sbjct: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNP 60

Query: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAV 120
           IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAV
Sbjct: 61  IDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAV 120

Query: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINI 180
           IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINI
Sbjct: 121 IQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINI 180

Query: 181 HDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTA 240
           HDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTA
Sbjct: 181 HDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTA 240

Query: 241 ITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300
           ITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND
Sbjct: 241 ITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNND 300

Query: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLN 360
           YTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLN
Sbjct: 301 YTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLN 360

Query: 361 GAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           GAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC
Sbjct: 361 GAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 407

BLAST of Moc08g00660 vs. ExPASy TrEMBL
Match: A0A6J1K9C7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491836 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 8.4e-219
Identity = 375/408 (91.91%), Postives = 393/408 (96.32%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGN 60
           MEI P F+ +I F TLLLQM SISSSPVADPE +V+EVL+SINNA +RR LGFLSCGTGN
Sbjct: 1   MEI-PSFLPIITF-TLLLQMCSISSSPVADPELVVQEVLSSINNATTRRNLGFLSCGTGN 60

Query: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHA 120
           PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGK+GKFYVVTDPSDKDVVNPKPGTLRHA
Sbjct: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHA 120

Query: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGIN 180
           VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQ+VTNIIIHGIN
Sbjct: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGIN 180

Query: 181 IHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGST 240
           IHDCKQGGNTYVRDSP+HYGWRTLSDGDGVSIFGG+H+WIDHCSLSNCRDGLIDAIHGST
Sbjct: 181 IHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGST 240

Query: 241 AITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300
           AIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 241 AITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300

Query: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLML 360
           DYTHWIMYAIGGSASPTINSQGNRYLAPN+++ KEVTK EDAPQS+WKNWNWRSSGDLML
Sbjct: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLML 360

Query: 361 NGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           NGAFFT SGAG+SSSYARASSLSAKSSSLV +MTA AGVLRCRKGSRC
Sbjct: 361 NGAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of Moc08g00660 vs. ExPASy TrEMBL
Match: A0A6J1HD44 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111462874 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.2e-218
Identity = 373/408 (91.42%), Postives = 393/408 (96.32%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGN 60
           MEI P F+ +I F TLLLQM +ISSSPVADP+ +V+EVL+SINNA +RR LGFLSCGTGN
Sbjct: 1   MEI-PSFLPIITF-TLLLQMCAISSSPVADPQLVVQEVLSSINNATTRRNLGFLSCGTGN 60

Query: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHA 120
           PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGK+GKFYVVTDPSDKDVVNPKPGTLRHA
Sbjct: 61  PIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKNGKFYVVTDPSDKDVVNPKPGTLRHA 120

Query: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGIN 180
           VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQ+VTNIIIHGIN
Sbjct: 121 VIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQYVTNIIIHGIN 180

Query: 181 IHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGST 240
           IHDCKQGGNTYVRDSP+HYGWRTLSDGDGVSIFGG+H+WIDHCSLSNCRDGLIDAIHGST
Sbjct: 181 IHDCKQGGNTYVRDSPEHYGWRTLSDGDGVSIFGGSHVWIDHCSLSNCRDGLIDAIHGST 240

Query: 241 AITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300
           AIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 241 AITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 300

Query: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLML 360
           DYTHWIMYAIGGSASPTINSQGNRYLAPN+++ KEVTK EDAPQS+WKNWNWRSSGDLML
Sbjct: 301 DYTHWIMYAIGGSASPTINSQGNRYLAPNNRDRKEVTKREDAPQSKWKNWNWRSSGDLML 360

Query: 361 NGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           NGAFFT SGAG+SSSYARASSLSAKSSSLV +MTA AGVLRCRKGSRC
Sbjct: 361 NGAFFTRSGAGSSSSYARASSLSAKSSSLVSTMTAGAGVLRCRKGSRC 406

BLAST of Moc08g00660 vs. ExPASy TrEMBL
Match: A0A0A0LKL0 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G350210 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 3.8e-211
Identity = 354/403 (87.84%), Postives = 381/403 (94.54%), Query Frame = 0

Query: 8   VLLIIFATLLLQMSSI-SSSPVADPETIVEEVLTSINNA--SRRKLGFLSCGTGNPIDDC 67
           + L++ + ++LQMSS+ SSSPV DP+ + +EVLTSINNA  +RR LGFLSC TGNPIDDC
Sbjct: 13  IKLLVLSIVMLQMSSMSSSSPVPDPDLVAQEVLTSINNATITRRNLGFLSCKTGNPIDDC 72

Query: 68  WRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQKE 127
           WRCD NWEKNRK+LADC IGFGKRAIGGK+GK+Y+VTDPSD DVVNPKPGTLRHAVIQKE
Sbjct: 73  WRCDANWEKNRKKLADCAIGFGKRAIGGKNGKYYIVTDPSDNDVVNPKPGTLRHAVIQKE 132

Query: 128 PLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHDCK 187
           PLWITFKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQFVTNIIIHGINIHDCK
Sbjct: 133 PLWITFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNIIIHGINIHDCK 192

Query: 188 QGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAITIS 247
           QGGNTYVRDSP+HYGWRT+SDGDG+SIFGG+H+WIDHCSLSNCRDGLIDAIHGSTAIT+S
Sbjct: 193 QGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDAIHGSTAITVS 252

Query: 248 NNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307
           NNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 253 NNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 312

Query: 308 IMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGAFF 367
           IMYAIGGSASPTINSQGNRYLAPN+QN+KEVTK ED PQS+WKNWNWRS GDLMLNGAFF
Sbjct: 313 IMYAIGGSASPTINSQGNRYLAPNNQNSKEVTKREDTPQSRWKNWNWRSDGDLMLNGAFF 372

Query: 368 TMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
             SGAGASSSYARASSLSAKSSSL+ S+TA AGVL+CRKGSRC
Sbjct: 373 VRSGAGASSSYARASSLSAKSSSLINSITAGAGVLKCRKGSRC 415

BLAST of Moc08g00660 vs. ExPASy TrEMBL
Match: A0A1S3BB18 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487738 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.1e-209
Identity = 355/413 (85.96%), Postives = 383/413 (92.74%), Query Frame = 0

Query: 4   NPIFVLL-------IIFATLLLQMSSI-SSSPVADPETIVEEVLTSINNA-SRRKLGFLS 63
           NP FV++       ++F+ ++LQM SI SSSPV DP+ + +EVLTSINNA +RR LGFLS
Sbjct: 3   NPKFVVIMIISIKVLVFSMVMLQMCSISSSSPVPDPDLVAQEVLTSINNATTRRNLGFLS 62

Query: 64  CGTGNPIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPG 123
           C TGNPIDDCWRCD NWEKNRKRLA+C IGFGKRAIGGK+G++Y+VTDPSD DVVNPKPG
Sbjct: 63  CRTGNPIDDCWRCDANWEKNRKRLANCAIGFGKRAIGGKNGRYYIVTDPSDNDVVNPKPG 122

Query: 124 TLRHAVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNII 183
           TLRHAVIQKEPLWI FKRDMVIKLKAELLMNSFKTIDGRG SVHIAGGPCITIQFVTNII
Sbjct: 123 TLRHAVIQKEPLWIIFKRDMVIKLKAELLMNSFKTIDGRGVSVHIAGGPCITIQFVTNII 182

Query: 184 IHGINIHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDA 243
           IHGINIHDCKQGGNTYVRDSP+HYGWRT+SDGDG+SIFGG+H+WIDHCSLSNCRDGLIDA
Sbjct: 183 IHGINIHDCKQGGNTYVRDSPEHYGWRTVSDGDGISIFGGSHVWIDHCSLSNCRDGLIDA 242

Query: 244 IHGSTAITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 303
           IHGSTAIT+SNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF
Sbjct: 243 IHGSTAITVSNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 302

Query: 304 HVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSS 363
           HVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPN+Q+ KEVTK EDAPQS+WKNWNWRS 
Sbjct: 303 HVVNNDYTHWIMYAIGGSASPTINSQGNRYLAPNNQDRKEVTKREDAPQSKWKNWNWRSD 362

Query: 364 GDLMLNGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           GDLMLNGAFF  SGAGASS YARASSLSAKSSSLV S+T+ AGVL+CRKGSRC
Sbjct: 363 GDLMLNGAFFVRSGAGASSRYARASSLSAKSSSLVSSITSGAGVLKCRKGSRC 415

BLAST of Moc08g00660 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 680.2 bits (1754), Expect = 9.9e-196
Identity = 327/409 (79.95%), Postives = 361/409 (88.26%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSS--ISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTG 60
           M +  + + L +F+ LLL +S   I+S+PV++PE +V+EV   I NASRR LG LSCGTG
Sbjct: 1   MRMTLVHLSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKI-NASRRNLGVLSCGTG 60

Query: 61  NPIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRH 120
           NPIDDCWRCDP WEKNR+RLADC IGFGK AIGG+DGK YVVTD SDKDVVNPKPGTLRH
Sbjct: 61  NPIDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRH 120

Query: 121 AVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGI 180
           AVIQ EPLWI F RDMVIKLK EL+MNSFKTIDGRGASVHIAGG CIT+Q+VTNIIIHG+
Sbjct: 121 AVIQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGV 180

Query: 181 NIHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGS 240
           NIHDCK+ GN YVRDSP HYGWRT SDGD VSIFGG+H+W+DHCSLSNC DGLIDAIHGS
Sbjct: 181 NIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGS 240

Query: 241 TAITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 300
           TAITISNNY++HHNKVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN
Sbjct: 241 TAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 300

Query: 301 NDYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLM 360
           NDYTHW MYAIGGSA+PTINSQGNR+LAPND   KEVTK+EDAP+S+WK WNWRS GDL 
Sbjct: 301 NDYTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLF 360

Query: 361 LNGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           LNGAFFT SG GASSSYA+ASSLSA+ SSLV S+T+ AG L CRKGSRC
Sbjct: 361 LNGAFFTPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of Moc08g00660 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 664.1 bits (1712), Expect = 7.3e-191
Identity = 311/400 (77.75%), Postives = 356/400 (89.00%), Query Frame = 0

Query: 11  IIFATLLLQMSSISSSPVADPETIVEEVLTSINNA-SRRKLGFLSCGTGNPIDDCWRCDP 70
           +IF   +L  +  SS PV+DPE +VEEV   IN + SRRKLGF SCG+GNPIDDCWRCD 
Sbjct: 33  LIFFCCILFSALASSLPVSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDDCWRCDK 92

Query: 71  NWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPLWIT 130
           +WEKNRKRLADCGIGFGK AIGG+DG+ YVVTDP + D VNP+PGTLR+AVIQ EPLWI 
Sbjct: 93  DWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWII 152

Query: 131 FKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHDCKQGGNT 190
           FKRDM I+LK EL+MNSFKT+DGRGASVHI+GGPCITIQ+VTNIIIHG++IHDCKQGGNT
Sbjct: 153 FKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNT 212

Query: 191 YVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAITISNNYMT 250
           YVRDSP+HYG+RT+SDGDGVSIFGG+H+W+DHCSLSNC DGLIDAI GSTAITISNNY+T
Sbjct: 213 YVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLT 272

Query: 251 HHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIMYAI 310
           HHNKVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW MYAI
Sbjct: 273 HHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAI 332

Query: 311 GGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGAFFTMSGA 370
           GGSA+PTINSQGNR+LAP+D ++KEVTKHEDAP+ +W+NWNWRS GDL+LNGAFFT SGA
Sbjct: 333 GGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGA 392

Query: 371 G--ASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           G   SSSY++ASSL+A+ SS VG +T  +G L C++GS C
Sbjct: 393 GPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Moc08g00660 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-188
Identity = 311/409 (76.04%), Postives = 357/409 (87.29%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINN--ASRRKLGFLSCGTG 60
           M+   +F+ ++ F   LL      SSPV DPE++VEEV  SIN   A RRKLG+LSC TG
Sbjct: 3   MQTKKLFITIVSF---LLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTG 62

Query: 61  NPIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRH 120
           NPIDDCWRCDP+WE++R+RLADC IGFGK AIGG+DG+ YVVTD  + + V+PKPGTLRH
Sbjct: 63  NPIDDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRH 122

Query: 121 AVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGI 180
           AV+Q EPLWI F+RDM I+LK EL+MNSFKTIDGRGASVHI+GGPCITIQ+VTNIIIHGI
Sbjct: 123 AVVQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGI 182

Query: 181 NIHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGS 240
           +IHDCKQGGN  VR SP H+GWRT+SDGDGVSIFGG+H+W+DHCS SNC DGLIDAI GS
Sbjct: 183 HIHDCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGS 242

Query: 241 TAITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 300
           TAIT+SNN+MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN
Sbjct: 243 TAITLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 302

Query: 301 NDYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLM 360
           NDYTHW MYAIGGSA+PTINSQGNR+LAPN + +KEVTKHEDAP+S+WK WNWRSSGDL+
Sbjct: 303 NDYTHWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLL 362

Query: 361 LNGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           LNGAFFT SG  ASSSYA+ASSL AK SSLVG +T+T+G L CRKGSRC
Sbjct: 363 LNGAFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Moc08g00660 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-188
Identity = 311/409 (76.04%), Postives = 357/409 (87.29%), Query Frame = 0

Query: 1   MEINPIFVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINN--ASRRKLGFLSCGTG 60
           M+   +F+ ++ F   LL      SSPV DPE++VEEV  SIN   A RRKLG+LSC TG
Sbjct: 3   MQTKKLFITIVSF---LLYAPLFLSSPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTG 62

Query: 61  NPIDDCWRCDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRH 120
           NPIDDCWRCDP+WE++R+RLADC IGFGK AIGG+DG+ YVVTD  + + V+PKPGTLRH
Sbjct: 63  NPIDDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRH 122

Query: 121 AVIQKEPLWITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGI 180
           AV+Q EPLWI F+RDM I+LK EL+MNSFKTIDGRGASVHI+GGPCITIQ+VTNIIIHGI
Sbjct: 123 AVVQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGI 182

Query: 181 NIHDCKQGGNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGS 240
           +IHDCKQGGN  VR SP H+GWRT+SDGDGVSIFGG+H+W+DHCS SNC DGLIDAI GS
Sbjct: 183 HIHDCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGS 242

Query: 241 TAITISNNYMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 300
           TAIT+SNN+MTHH+KVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN
Sbjct: 243 TAITLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 302

Query: 301 NDYTHWIMYAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLM 360
           NDYTHW MYAIGGSA+PTINSQGNR+LAPN + +KEVTKHEDAP+S+WK WNWRSSGDL+
Sbjct: 303 NDYTHWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLL 362

Query: 361 LNGAFFTMSGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           LNGAFFT SG  ASSSYA+ASSL AK SSLVG +T+T+G L CRKGSRC
Sbjct: 363 LNGAFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Moc08g00660 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 621.7 bits (1602), Expect = 4.2e-178
Identity = 295/401 (73.57%), Postives = 336/401 (83.79%), Query Frame = 0

Query: 7   FVLLIIFATLLLQMSSISSSPVADPETIVEEVLTSINNASRRKLGFLSCGTGNPIDDCWR 66
           F+LL+     L   SS+    V DP  +V++V  S+ NASRR L +LSC TGNPIDDCWR
Sbjct: 13  FLLLLTIGNTLAFSSSLPDH-VQDPNLVVDDVNRSVFNASRRSLAYLSCRTGNPIDDCWR 72

Query: 67  CDPNWEKNRKRLADCGIGFGKRAIGGKDGKFYVVTDPSDKDVVNPKPGTLRHAVIQKEPL 126
           CDPNWE NR+RLADC IGFGK AIGG+ G+ YVVTDP++ D VNP+PGTLR+AV Q+EPL
Sbjct: 73  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 132

Query: 127 WITFKRDMVIKLKAELLMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGINIHDCKQG 186
           WI FKRDMVI+LK EL++ SFKTIDGRG+SVHI  GPC+ I + TNIIIHGINIHDCK G
Sbjct: 133 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 192

Query: 187 GNTYVRDSPDHYGWRTLSDGDGVSIFGGTHIWIDHCSLSNCRDGLIDAIHGSTAITISNN 246
               ++D P H GW   SDGD V+IFGG H+WIDHCSLSNC DGLIDAIHGSTAITISNN
Sbjct: 193 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 252

Query: 247 YMTHHNKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWIM 306
           +MTHH+KVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW M
Sbjct: 253 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 312

Query: 307 YAIGGSASPTINSQGNRYLAPNDQNNKEVTKHEDAPQSQWKNWNWRSSGDLMLNGAFFTM 366
           YAIGGSASPTI SQGNR+LAPN + NKEVTKHEDAP+S+W++WNWRS GD++LNGA+F  
Sbjct: 313 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 372

Query: 367 SGAGASSSYARASSLSAKSSSLVGSMTATAGVLRCRKGSRC 408
           SGA A S+YARASSLSA+ SSLVGS+T TAG L CR+G RC
Sbjct: 373 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131699.16.8e-239100.00probable pectate lyase 5 [Momordica charantia][more]
XP_022996659.11.7e-21891.91probable pectate lyase 5 [Cucurbita maxima][more]
KAG7029228.13.9e-21891.67putative pectate lyase 5 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023545498.18.6e-21891.42probable pectate lyase 5 [Cucurbita pepo subsp. pepo][more]
XP_022962436.18.6e-21891.42probable pectate lyase 5 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FXD81.4e-19479.95Probable pectate lyase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g67750 PE=2 SV=2[more]
Q93Z251.0e-18977.75Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Q9C5M82.2e-18776.04Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
Q9LTZ05.9e-17773.57Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2[more]
O245541.3e-17676.20Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BR073.3e-239100.00Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111004807 PE=3 SV=1[more]
A0A6J1K9C78.4e-21991.91Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491836 PE=3 SV=1[more]
A0A6J1HD444.2e-21891.42Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111462874 PE=3 SV=1[more]
A0A0A0LKL03.8e-21187.84Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G350210 PE=3 SV=1[more]
A0A1S3BB182.1e-20985.96Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487738 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G67750.19.9e-19679.95Pectate lyase family protein [more]
AT5G63180.17.3e-19177.75Pectin lyase-like superfamily protein [more]
AT4G24780.11.5e-18876.04Pectin lyase-like superfamily protein [more]
AT4G24780.21.5e-18876.04Pectin lyase-like superfamily protein [more]
AT3G27400.14.2e-17873.57Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 349..373
score: 52.19
coord: 378..401
score: 38.69
coord: 141..157
score: 69.75
coord: 81..98
score: 57.67
coord: 105..130
score: 66.3
coord: 285..304
score: 78.1
coord: 205..226
score: 61.26
coord: 307..326
score: 74.52
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 133..330
e-value: 3.4E-103
score: 358.8
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 144..322
e-value: 6.8E-19
score: 68.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 58..400
e-value: 3.8E-117
score: 393.1
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 10..404
NoneNo IPR availablePANTHERPTHR31683:SF151PECTATE LYASEcoord: 10..404
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 42..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g00660.1Moc08g00660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity