Moc08g00390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g00390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	pectinesterase inhibitor 12-like
Location	chr8: 244238 .. 244777 (+)
RNA-Seq Expression	Moc08g00390
Synteny	Moc08g00390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTCAAGCTTGTTTGTTTTTTGTGGCACTACTAATGGCCGGTATGTGGTACGGCGGGCATGCACAGGGCCAGAGCCAGGAGCGCGAGCGCGAGCTTCTGGGTCTGAGTATCAATTTGAATCTGGGATTTGACATTTGCCGGAAGGCGGACTACCCAGCCATGTGTCGGTCCGTGGTGAAGGGGCTTAATAACCCGAGGGTTGCCTTCGAAGCTGCGGTTCGGCAATTGATGTTCCAGACCAGCCGGGCGAAGAGTGTGGCCCAGAGGAACAAAGACAAGTCGTCGGCCATGGAAGTGTGCTTCGAGGTCTATGATGACGCCTACTCCAACCTGGAGACCGGCCTTTCAAACCTCAAGAGCCACGACAAGTCTAGCCTCAACGTTGACCTCTCTGCCGTTCTCACCGATTATGTCACCTGCGACGATGCCATCAACGAAGAAGGCATTACTTCTCCCGTTACCCGCACCAACGCTATACTCAGCCAAATGGCCTCCAATTGTCTCTATTTCTCCAGTTTGATTCACTTACGCTAA

mRNA sequence

ATGGCTTCTCAAGCTTGTTTGTTTTTTGTGGCACTACTAATGGCCGGTATGTGGTACGGCGGGCATGCACAGGGCCAGAGCCAGGAGCGCGAGCGCGAGCTTCTGGGTCTGAGTATCAATTTGAATCTGGGATTTGACATTTGCCGGAAGGCGGACTACCCAGCCATGTGTCGGTCCGTGGTGAAGGGGCTTAATAACCCGAGGGTTGCCTTCGAAGCTGCGGTTCGGCAATTGATGTTCCAGACCAGCCGGGCGAAGAGTGTGGCCCAGAGGAACAAAGACAAGTCGTCGGCCATGGAAGTGTGCTTCGAGGTCTATGATGACGCCTACTCCAACCTGGAGACCGGCCTTTCAAACCTCAAGAGCCACGACAAGTCTAGCCTCAACGTTGACCTCTCTGCCGTTCTCACCGATTATGTCACCTGCGACGATGCCATCAACGAAGAAGGCATTACTTCTCCCGTTACCCGCACCAACGCTATACTCAGCCAAATGGCCTCCAATTGTCTCTATTTCTCCAGTTTGATTCACTTACGCTAA

Coding sequence (CDS)

ATGGCTTCTCAAGCTTGTTTGTTTTTTGTGGCACTACTAATGGCCGGTATGTGGTACGGCGGGCATGCACAGGGCCAGAGCCAGGAGCGCGAGCGCGAGCTTCTGGGTCTGAGTATCAATTTGAATCTGGGATTTGACATTTGCCGGAAGGCGGACTACCCAGCCATGTGTCGGTCCGTGGTGAAGGGGCTTAATAACCCGAGGGTTGCCTTCGAAGCTGCGGTTCGGCAATTGATGTTCCAGACCAGCCGGGCGAAGAGTGTGGCCCAGAGGAACAAAGACAAGTCGTCGGCCATGGAAGTGTGCTTCGAGGTCTATGATGACGCCTACTCCAACCTGGAGACCGGCCTTTCAAACCTCAAGAGCCACGACAAGTCTAGCCTCAACGTTGACCTCTCTGCCGTTCTCACCGATTATGTCACCTGCGACGATGCCATCAACGAAGAAGGCATTACTTCTCCCGTTACCCGCACCAACGCTATACTCAGCCAAATGGCCTCCAATTGTCTCTATTTCTCCAGTTTGATTCACTTACGCTAA

Protein sequence

MASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR

Homology

[Close]

BLAST of Moc08g00390 vs. NCBI nr
Match: XP_022131971.1 (pectinesterase inhibitor 12-like [Momordica charantia])

HSP 1 Score: 354.0 bits (907), Expect = 7.7e-94
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSV 60
           MASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSV
Sbjct: 1   MASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSV 60

Query: 61  VKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNL 120
           VKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNL
Sbjct: 61  VKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNL 120

Query: 121 KSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 180
           KSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR
Sbjct: 121 KSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 179

[Close]

BLAST of Moc08g00390 vs. NCBI nr
Match: XP_023536926.1 (uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 199.9 bits (507), Expect = 1.9e-47
Identity = 108/174 (62.07%), Postives = 132/174 (75.86%), Query Frame = 0


Query: 6   CLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSVVKGLN 65
           CL  VA  MAGMW GG  QGQ QE+ERELL L+++ N+G D+C KA+YP +CRSVVKGL+
Sbjct: 11  CLLLVA--MAGMWEGG--QGQGQEQERELLSLNLDPNMG-DLCGKAEYPVLCRSVVKGLS 70

Query: 66  NPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDK 125
            P VA E+A+RQLM QT  A++ A+R + KS  MEVC E Y DA+ NL+T LS LK+ DK
Sbjct: 71  KPSVALESAIRQLMLQTKMARTTAKRLR-KSPGMEVCSETYKDAFGNLKTCLSTLKTGDK 130

Query: 126 SSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 180
            +LN++LSA LT YVTCDDA+ E G TSPVTRTN  L QMA+NCLY SSLI +R
Sbjct: 131 PTLNINLSAALTGYVTCDDAVAERGGTSPVTRTNLTLRQMATNCLYLSSLIGVR 178

[Close]

BLAST of Moc08g00390 vs. NCBI nr
Match: XP_038886537.1 (uncharacterized protein LOC120076712 [Benincasa hispida])

HSP 1 Score: 196.1 bits (497), Expect = 2.7e-46
Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 0


Query: 39  INLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSA 98
           I+LNL  D+C++AD+PA+CRSVVKG+++P VA E+ ++QLMFQT +A +VA+R   KSSA
Sbjct: 32  ISLNLSLDLCQRADFPALCRSVVKGIHDPSVAIESTIKQLMFQTKQAMNVARRR--KSSA 91

Query: 99  MEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRT 158
           M+VC EVYDDAYSNLET LS+L+SHDK +LN++LSA LTDYVTCDDA+ E G+ SPVTRT
Sbjct: 92  MDVCIEVYDDAYSNLETCLSSLQSHDKGTLNINLSAALTDYVTCDDAVAEIGLISPVTRT 151

Query: 159 NAILSQMASNCLYFSSLIHLR 180
           N ++ +MASNCLY SSLIHLR
Sbjct: 152 NDLMRRMASNCLYLSSLIHLR 170

[Close]

BLAST of Moc08g00390 vs. NCBI nr
Match: KAG6585173.1 (hypothetical protein SDJN03_17906, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 192.2 bits (487), Expect = 3.9e-45
Identity = 103/166 (62.05%), Postives = 125/166 (75.30%), Query Frame = 0


Query: 14  MAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEA 73
           MAGMW G    GQ QE+ERELL L++  N+G D+C KADYP +CRSVVKGL+ P VA E+
Sbjct: 1   MAGMWEG----GQGQEQERELLSLNLYPNMG-DLCGKADYPVLCRSVVKGLSRPSVALES 60

Query: 74  AVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLS 133
           A+RQLM QT  A++ A+R + KS  MEVC E Y DA+ NL+T LS LK+ DK +LN++LS
Sbjct: 61  AIRQLMLQTKMARTTAKRLR-KSPGMEVCSETYQDAFRNLKTCLSTLKTGDKPTLNINLS 120

Query: 134 AVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 180
           A LT YVTCDDA+ E G TSPVTRTN  L QMA+NCLY SSLI +R
Sbjct: 121 AALTGYVTCDDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLIGVR 160

[Close]

BLAST of Moc08g00390 vs. NCBI nr
Match: XP_004143062.2 (pectinesterase inhibitor 12 [Cucumis sativus] >KGN62312.1 hypothetical protein Csa_018682 [Cucumis sativus])

HSP 1 Score: 191.8 bits (486), Expect = 5.0e-45
Identity = 94/142 (66.20%), Postives = 117/142 (82.39%), Query Frame = 0


Query: 37  LSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKS 96
           L++NLNL  D+C+KADYPA+CRSVVKGL +P  A E +++QLM +T +A SVA+R   KS
Sbjct: 38  LTLNLNLSLDLCKKADYPALCRSVVKGLVDPSTAMETSIKQLMVETKQAMSVAKR--QKS 97

Query: 97  SAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVT 156
           SAM+VC EVYDDAYSNLET LS+LKSHDK +LN++LSA LTDYVTC DAI E+G++SP+T
Sbjct: 98  SAMDVCIEVYDDAYSNLETCLSSLKSHDKGTLNINLSAALTDYVTCQDAIAEKGLSSPIT 157

Query: 157 RTNAILSQMASNCLYFSSLIHL 179
           R N +LSQM +NCLY S LI L
Sbjct: 158 RNNNLLSQMTTNCLYLSGLIRL 177

[Close]

BLAST of Moc08g00390 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 4.3e-07
Identity = 44/172 (25.58%), Postives = 79/172 (45.93%), Query Frame = 0


Query: 8   FFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKA---DYPAMCRSVVKGL 67
           F V+L++  ++  G A  Q+  ++      + +  L +D C  +   D  +   + ++ L
Sbjct: 3   FLVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESL 62

Query: 68  NNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHD 127
                   AA    +          QR +D    +++C   Y+DA  +L T L+N+KS D
Sbjct: 63  VLASTKTAAAKITNLKGIVAQDLKDQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSRD 122

Query: 128 KSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLI 177
               N++LSA L     C+D   E   TSP+T  N+IL +     L F++++
Sbjct: 123 YQGANINLSAALDVPGNCEDDFKEAKKTSPITNENSILFKTILIPLAFTNML 174

[Close]

BLAST of Moc08g00390 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 30/76 (39.47%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 102 CFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINE-EGITSPVTRTNA 161
           C  +Y DA S+++  +++ KS D +S NV +SA L D VTC+D   E +GI SPVT+ N 
Sbjct: 102 CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENK 161

Query: 162 ILSQMASNCLYFSSLI 177
              Q+ +  L  + L+
Sbjct: 162 DYVQLTAISLAITKLL 177

[Close]

BLAST of Moc08g00390 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 30/76 (39.47%), Postives = 46/76 (60.53%), Query Frame = 0


Query: 102 CFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINE-EGITSPVTRTNA 161
           C  +Y DA S+++  +++ KS D +S NV +SA L D VTC+D   E +GI SPVT+ N 
Sbjct: 93  CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENK 152

Query: 162 ILSQMASNCLYFSSLI 177
              Q+ +  L  + L+
Sbjct: 153 DYVQLTAISLAITKLL 168

[Close]

BLAST of Moc08g00390 vs. ExPASy TrEMBL
Match: A0A6J1BQY5 (pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 3.7e-94
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSV 60
           MASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSV
Sbjct: 1   MASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSV 60

Query: 61  VKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNL 120
           VKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNL
Sbjct: 61  VKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNL 120

Query: 121 KSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 180
           KSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR
Sbjct: 121 KSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 179

[Close]

BLAST of Moc08g00390 vs. ExPASy TrEMBL
Match: A0A0A0LNC2 (Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3659 GN=Csa_2G348980 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.4e-45
Identity = 94/142 (66.20%), Postives = 117/142 (82.39%), Query Frame = 0


Query: 37  LSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKS 96
           L++NLNL  D+C+KADYPA+CRSVVKGL +P  A E +++QLM +T +A SVA+R   KS
Sbjct: 38  LTLNLNLSLDLCKKADYPALCRSVVKGLVDPSTAMETSIKQLMVETKQAMSVAKR--QKS 97

Query: 97  SAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVT 156
           SAM+VC EVYDDAYSNLET LS+LKSHDK +LN++LSA LTDYVTC DAI E+G++SP+T
Sbjct: 98  SAMDVCIEVYDDAYSNLETCLSSLKSHDKGTLNINLSAALTDYVTCQDAIAEKGLSSPIT 157

Query: 157 RTNAILSQMASNCLYFSSLIHL 179
           R N +LSQM +NCLY S LI L
Sbjct: 158 RNNNLLSQMTTNCLYLSGLIRL 177

[Close]

BLAST of Moc08g00390 vs. ExPASy TrEMBL
Match: A0A5A7V5S8 (Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002070 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.2e-45
Identity = 94/142 (66.20%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 37  LSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKS 96
           L++NLNL  D+C+KADYPA+CRSVVKGL +P  A E ++++LM +T +A SVA+R   KS
Sbjct: 35  LTLNLNLSLDLCKKADYPALCRSVVKGLTDPSTAMETSIKKLMVETKQAMSVAKR--QKS 94

Query: 97  SAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVT 156
           SAM+VC EVYDDAYSNLET LS+LKSHDK +LN++LSA LTDYVTCDDAI E G TSPVT
Sbjct: 95  SAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNINLSASLTDYVTCDDAIAERGSTSPVT 154

Query: 157 RTNAILSQMASNCLYFSSLIHL 179
           R N +L +M +NCLY S LI L
Sbjct: 155 RNNKLLREMTTNCLYLSGLIRL 174

[Close]

BLAST of Moc08g00390 vs. ExPASy TrEMBL
Match: A0A1S3BAX0 (uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.2e-45
Identity = 94/142 (66.20%), Postives = 115/142 (80.99%), Query Frame = 0


Query: 37  LSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKS 96
           L++NLNL  D+C+KADYPA+CRSVVKGL +P  A E ++++LM +T +A SVA+R   KS
Sbjct: 35  LTLNLNLSLDLCKKADYPALCRSVVKGLTDPSTAMETSIKKLMVETKQAMSVAKR--QKS 94

Query: 97  SAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVT 156
           SAM+VC EVYDDAYSNLET LS+LKSHDK +LN++LSA LTDYVTCDDAI E G TSPVT
Sbjct: 95  SAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNINLSASLTDYVTCDDAIAERGSTSPVT 154

Query: 157 RTNAILSQMASNCLYFSSLIHL 179
           R N +L +M +NCLY S LI L
Sbjct: 155 RNNKLLREMTTNCLYLSGLIRL 174

[Close]

BLAST of Moc08g00390 vs. ExPASy TrEMBL
Match: A0A6J1KPW1 (pectinesterase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111496527 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 4.6e-44
Identity = 107/174 (61.49%), Postives = 129/174 (74.14%), Query Frame = 0


Query: 6   CLFFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSVVKGLN 65
           CL  VA   AGMW GG  QGQ    ERELL    +LN+G D+C KA+YP +CRS+VKGL+
Sbjct: 11  CLLLVA--TAGMWQGG--QGQ----ERELL----SLNMG-DLCGKANYPVLCRSMVKGLS 70

Query: 66  NPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDK 125
           NP VA E+A+RQLM QT  A++ A+R + KS  MEVC E Y DA+SNL+T LS LK+ DK
Sbjct: 71  NPSVALESAIRQLMLQTKMARTTARRLR-KSPGMEVCSETYQDAFSNLKTCLSTLKTGDK 130

Query: 126 SSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 180
            +LN++LSA LTDYVTCDDA+ E G TSPVTRTN  L QMA+NCLY SSLI +R
Sbjct: 131 PTLNINLSAALTDYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 170

[Close]

BLAST of Moc08g00390 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.3e-24
Identity = 54/140 (38.57%), Postives = 88/140 (62.86%), Query Frame = 0


Query: 37  LSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKS 96
           LSI+L    D C  +D+PA+CRS +KG  N   A + A+++LM +T +AK +A++   + 
Sbjct: 21  LSISL---ADPCTVSDFPALCRSTIKGQTNVNAATDVAIKELMKRTRQAKKIAEKELKRD 80

Query: 97  SAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVT 156
             +  C   ++ A+ NLE  L+N+K +D  SLN++LSA LTDY TC D++ E    + + 
Sbjct: 81  GGVATCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDYDTCSDSMKETKEVNVIY 140

Query: 157 RTNAILSQMASNCLYFSSLI 177
           ++  +L +MA NCL  S+L+
Sbjct: 141 KSAGVLYRMADNCLALSTLV 157

[Close]

BLAST of Moc08g00390 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 3.4e-23
Identity = 52/133 (39.10%), Postives = 82/133 (61.65%), Query Frame = 0


Query: 44  GFDICRKADYPAMCRSVVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCF 103
           G D C  +D+  +CRSVVKG  N   A E ++R+LM +T +AK  A+ ++     ++ C+
Sbjct: 33  GRDPCSVSDFKVLCRSVVKGQKNVNAATEVSIRELMKRTIKAKEAAKISRKSGGGLKTCY 92

Query: 104 EVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILS 163
             YD A  NL+  L N+K +D  SLN++LSA LTD+ TC+DA+     ++   ++ + L 
Sbjct: 93  SNYDSALENLQKALKNIKQNDGFSLNINLSASLTDFDTCNDAMGGGTASNVFAKSTSTLH 152

Query: 164 QMASNCLYFSSLI 177
           +MA NCL  S+L+
Sbjct: 153 EMADNCLALSTLV 165

[Close]

BLAST of Moc08g00390 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 2.6e-15
Identity = 52/132 (39.39%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 47  ICRKADYPAMCRSVVKGLNNPRV-AFEAAVRQLMFQTS-RAKSVAQRNKDKSSAMEVCFE 106
           IC K D P +C S V  L  P++    ++V  L  Q S  A   A    +K  A + C E
Sbjct: 133 ICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEKVDASD-CQE 192

Query: 107 VYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQ 166
           +YDDA  NLE  ++ +KS D +++N +LSA +TDY TC+D   E G  +P+      L++
Sbjct: 193 LYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAYVADKLTK 252

Query: 167 MASNCLYFSSLI 177
           M SNCL  S+LI
Sbjct: 253 MVSNCLAISTLI 263

[Close]

BLAST of Moc08g00390 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 3.1e-08
Identity = 44/172 (25.58%), Postives = 79/172 (45.93%), Query Frame = 0


Query: 8   FFVALLMAGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKA---DYPAMCRSVVKGL 67
           F V+L++  ++  G A  Q+  ++      + +  L +D C  +   D  +   + ++ L
Sbjct: 3   FLVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESL 62

Query: 68  NNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHD 127
                   AA    +          QR +D    +++C   Y+DA  +L T L+N+KS D
Sbjct: 63  VLASTKTAAAKITNLKGIVAQDLKDQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSRD 122

Query: 128 KSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLI 177
               N++LSA L     C+D   E   TSP+T  N+IL +     L F++++
Sbjct: 123 YQGANINLSAALDVPGNCEDDFKEAKKTSPITNENSILFKTILIPLAFTNML 174

[Close]

BLAST of Moc08g00390 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 2.7e-04
Identity = 38/155 (24.52%), Postives = 64/155 (41.29%), Query Frame = 0


Query: 46  DICRKADYPAMC-RSVVKGLNNP----------------RVAFEAAVRQLMFQTSRAKSV 105
           D+C+  DY   C  +++  L                   R+  E+ +++       AK V
Sbjct: 128 DLCKNTDYNNECIAAILPDLQKQAEGEGGDGGIAPKDVMRMEAESLLKKANATLDYAKRV 187

Query: 106 AQRNKDK---SSAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSAVLTDYVTC-DD 165
            +  K       AM VC E YD   S LE     ++  D   L+  LSA ++D  TC D+
Sbjct: 188 VEDRKTPMPVKEAMSVCVENYDSLISGLEDAKMAMEEGDYGRLDSVLSAAISDVSTCSDN 247

Query: 166 AINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 180
            ++   + SP    + ++ ++ SN L  S  +  R
Sbjct: 248 FVDIPDVDSPTASLDDLMKKLCSNVLAMSQKVQNR 282

The following BLAST results are available for this feature:

BLAST of Moc08g00390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131971.1	7.7e-94	100.00	pectinesterase inhibitor 12-like [Momordica charantia]	[more]
XP_023536926.1	1.9e-47	62.07	uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo]	[more]
XP_038886537.1	2.7e-46	67.38	uncharacterized protein LOC120076712 [Benincasa hispida]	[more]
KAG6585173.1	3.9e-45	62.05	hypothetical protein SDJN03_17906, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_004143062.2	5.0e-45	66.20	pectinesterase inhibitor 12 [Cucumis sativus] >KGN62312.1 hypothetical protein C...	[more]

BLAST of Moc08g00390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJR5	4.3e-07	25.58	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
Q8GT41	1.6e-06	39.47	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	1.6e-06	39.47	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]

BLAST of Moc08g00390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQY5	3.7e-94	100.00	pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 ...	[more]
A0A0A0LNC2	2.4e-45	66.20	Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3...	[more]
A0A5A7V5S8	3.2e-45	66.20	Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa ...	[more]
A0A1S3BAX0	3.2e-45	66.20	uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=...	[more]
A0A6J1KPW1	4.6e-44	61.49	pectinesterase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111496527 PE=4 SV=1	[more]

BLAST of Moc08g00390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23350.1	2.3e-24	38.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70540.1	3.4e-23	39.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	2.6e-15	39.39	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	3.1e-08	25.58	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47340.1	2.7e-04	24.52	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 39..172 e-value: 2.5E-20 score: 83.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 77..176 e-value: 1.0E-13 score: 49.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 46..170 e-value: 1.1E-18 score: 68.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 45..177 e-value: 3.5E-22 score: 80.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 46..176
None	No IPR available	PANTHER	PTHR31707:SF270	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 43..176
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 43..176
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 46..176 e-value: 3.14609E-44 score: 141.728

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g00390.1	Moc08g00390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity