Moc08g00200 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g00200
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptiontype 2 DNA topoisomerase 6 subunit B-like isoform X1
Locationchr8: 114650 .. 123635 (+)
RNA-Seq ExpressionMoc08g00200
SyntenyMoc08g00200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAAAACAAGTATATCCATCGCCCAGAATGAGAGGAACGATGTTCTAGAAGCATTTAATCCTGCAATATCCAATTTCCTTGCGCAGCTTTCTCAGAAAGTTCAAAATTCTCTGAAGGTTGGTTAGTTATCTTTATGATATTAAATAAACTTCTGTTTGCAAACAAGTATAAGAAATAAAATTGAAACAAAAATGTTTAAAGTTTTTGTGTGGATGTTGGACAGAAATGATAAGATAATGAACATTTTGTTGTTATGATTAGTTACCTTTTAAATTGAAATGCAATCTGGAAATTTTAGACTCTTTTATATATATATGTATGTATGTATTTAACAAAATACTTCTATATAAAGGTGGCCATGTAACAAAAGGAGCAATACACTTCAAATCTATTATAGGAAGTTCACTCTCTCCCTCTGTTTGTTTTTATTTATTTATTTCTTTTTTTTTTTTTTTTTTGGTCGCAGCCACAGCTTAATAGATTGGCAAATGAAGGTCAGAAATTGACGTCAATGAGTCCATTCGCAAAAGGGGAGAGGAAGGGATCTTCCACATCATCCTTGAAGCTTGATGTGGAGAAGCAACTGCAGGCATGGAGAGAGAACCCTTCATGGACTGACAAACCTCCACAAATAAAGGTGCGCAATATAAAGATAGAACTAGAAGGATATGCTTTCCATTATAGACCTGATGTTCGGAAAATTGGTTATCCTTTTCTGAACTTGCAGTTGTAAGAAAAGAATATGGTCTTGCAAGGGTCCCTTTCCCCCTCCAATGTATTCATGAAGTGCATTGTCGATCAAGTGTTATGTGTTATGTATTGTGAATCTGAAGTTGACATGGGGAGGGGAATGGAATTTACTCTTCCAAGCTCTTTATCTGCTTCAGTTAGGATTTCATTAAATAGAGCATTACCTAACAACTCAGTTTTTAAAATTTAAGGGAGAACAATATTTTCTCCCTTAAAATGTCATCCTTTCAGTTGTAATTTTGAAATGCACTCCGGGGCCTTTCATCTTTATTTAATTTTGAAGGTTAAGCCTTTGATATTATTTGTATGCATTTGAGTCATTAAGGATGAATTCTCTTGCAACTAAATTTCTGCCTTTGTCATTTAAGCTATTGATTTGGATTCTTAGCCATAGAGCATTTTTTAATTTCATTTGAAGAAGGATATTTGATTCATTTTTTAAGTATGGCTTTTTTTTTACAATTTAAATTATTGAATTCATGGTCTAAAATCACGTGAGTGAGTGAAATTCTGTTGCATGTCATGTGCCTAGTTTCAACATAAATGTGATTGATGTGACAAACAGGTTTAAAATGTAAAGAAGTAAAAGTTTAAGGACATAGTTAAAACAGAAATAAAAAGGTCAAATCTGGAACATATCATTGATGGAAGAATAAAATGAAGTTCATCTCAAATTTTAATGGTAAGTAAACACATGGTTTCTGTATTTGGCTTTAAGTTGATTGCATGCATATGCAGTTGGCTCGTAAATAATTCTGAAAACTTAAGCTAGAAGATTGATTGTAAGTGGTGGTTAGTAACAAATGCATTAGATGCCAGTATCAACCATTTTCTCACAGGATACTGAGGTTGGTCAGGTTTAAATGAGACTCTTTGCAGGTAACTGTACCGAAAGATACCCTTAGCAGACTCAATGCAAAGGTTGATGTTGGTTTGCCACCAGATGCTGTCTATAATATCGTGACAGACCCTGATAACAAGAGAGTTTTCAAGAATATTAAAGTAAGCTACACATTTTCAATCAGAAATCCTCTGTTTTCTGTCTTCTCAGATAAGCATATATATATATATATATATATATAATATGGGCATCAGTTAGTGTTCTGCATCTTGTTATAACTATATCACCTTCTGAGTTTAGAAGATTAATGTCTGTTTCAGGAGGTGATATCCAGAAAGGTTTTGATTGATGAAGGCTCGAGACAAGTGGTTGAGGTGGAGCAAGCAGCTTTGTGGAGATTTCTTTGGTGGTCCGGGACCATTTCAGTTCATGTTCTAGTAGATCAAAACAGAGCGGATCACTCAGTAAGCACTTAACATTTCTTTTGTTCTTAAGAATATACAGACTACTTCTAGGATATTTTAAAGGAAGAAAGCAAATTCTCCATTTCCTAATGAGTGTGAAATAGCATATCCATTTACTTTCAGGAGTTCCTTATTTGAATGCTTGCTAGTTCAATACTTGATATTAATGCCTCAAATTGAACTTTTTCATCCAACACATGGATACAGTTTAGACCGTAGCGCTTTTTACAGTCATATTTTGTTGTGACTTCAATGATGATCATCGGCCTAACAATGAAAGATTAGTGAAAAGAAAACTTGTTGATTCAATTATGGATTCAATATTTTTAGATGAAGTTCAAGCAACTGAAGACTGGATTTATGAAGAAATTCGAAGGCTGCTGGAGAGTAGAGCCTATATTTGTTGATGAAAGCGTCTGCTTTCCAGTAAAACCCAAGAATTTGACAGATTATCATGCTTGTACAAAGGGCAAGGGAAGAATAGGGTCAAGGGTGAGCCTCGAACAGCTGATCCAGCCAGCCATTGTTCCACCACCGCCCATCTCCTGGTATCTTCGCGGAATTACCACCAGGACCACTGAGATGCTCATTCTTGATCTGCTTGCTGAAGCCAAAAGGATAAGGGAAGATGCCAAAGGTCAAACTTTGAACAATGAGCTTGATATTTCTCAGGGAACGTGTGATAGCAACTTATTAGATAGCATTCTTGATATTAAAGAAAGATGGGCAATGCATAGGAGGAATGCGAAGCCATGTCGTAGGAGGCTCGGTGCTACCGAATGAATTTGTAATAGCTTACATCCATTTTTAAGCCTGTAGTATGTATTACAAACGGATTTCACTATACCTTTTCCAGCTATTTGTCACTCCTGCATGGTCAAACTGACTGGTCTGAAATTTGGAGATCAGTAGAACAGCCTCTAGGACGACAAGGAGCTTTTCACATAAAATATTTTCTTGTCAAAGCAAGCATAATTAAGTAGTAATTGACATATATCTCCAATCAAGGGGTGAGTTCAATCCTCCATCCCACGTATTGTACTAAAAAAAACATAAAATATTTTCTTTTACATGCTACAAAAACTTCCACTACTTCCGTTAATGAGTAACTTGTGCCTCAAAGTAAAATGAAATGAAAAAAAAATCACTTGTACGCATCTATGGTTCCAAATAAATGACACTGTATTCCTCCTTAGAAAGGAACGACAATTTCAACTATTATAAGAAGTAGAACATATGTGATTATTAATTAAAAAGAAATAAGGAAAAAATACTTGAAAGAAAGGTTATTCAAATATATATAATTTTCGTAGTTCTTTCTTGTCTTTCCCCTCCCTTTTTCTAAGTTTTTTTCTCATTCTTTTCACTCGTGCGTTTAATTGCCTTTGCAAGAACCTGAATCTGGGATCAAAGGCCAGTAAACATCAGTGATCAGAGTTGGTTGGGTGGGCCCTATTGGAAACTTTGCTTGTGTTAGCGAGCCGAGCCGAGCCGGGCCCAACTCAGAGCGTTCTTAAAAATCATAACTACTCTTTGAATTAGAAGCAGTTGTGCATGTTTATTTGCAGGAGCGCGGGAGTCTGTTTCTAGTCTTCTCCCAAAACTTCTACCATGGAGTTTAATTCGCTTCAAGACCTCTTCCTTCTTGTAAGTTCAATCTTGAGGAACCGTGACCGATTTCTTTCAATAAGGAAATAGAGACTCCAAGCACGAGAGACATTTTAAAGAAACTTCTTCCTCTCAACTGTCTATTGCAGCTAATTTCTTCAGCAATACGGAGATGCATCTGCTCCGAACGACTTTGCAGACTCTCAGTTCTTCTGCAACGCTCTGCCGCTTCCGATCCTCCCCTAGTCTGTATTTCTAGTGAGTGATCTCTCGCTCTCTTTCTCGGCGGTGGTTGTCCAAGTACTCGTAATCTCATTCTAGTTCTATTCAATTTTGGTTTATCAAGAATTTCGACGACTTTGTTCTCAGTCGGTTCATGAACTACTTTCGATGAATTTAATTTTCTGGTCCTCCACTAGTTTGCATTTCAAGTGAGGGATGTCTCGCGCTCTTTCTCGTCGGTGATTGTCGAGATACTCGTTATCTCATTTTAGTTCTATTCAATTTTGGTTTATCAAACATTTTGACGACTTTAGGTCTTAGTCAGTTCATTAACTACTTTCGATTCAAAGAATTTTAATGAGGCATTGAAACTTCAAAAGTTACAAAGAAACCTTCTAATGAGAGTAAATCCTGGAATTTTTCGATCTAGTTTCAGATACCGGCCTTGGAAGCTGCCTAGAGGAGTTTCAGGATTTTAAGTGTCCCGTTGAAGGTATCCTTGCTGAACAGTGGGGTGAGTTGCTGACTCTGCTCCACTAGCTCTCTGGCAGTACTTGATAAACAGTAAAATGCTTTAATTAATTCCTCTACGTTATTTGATTACCGTTTGTGTACTATTTTGTTGATTTTTTCGTCTGCATTACGTTCATTGCAGTAGCTCATGTACAGAGGTAGCTACCTTATTCTTTTCCTGAGCATATGCTCATATGGTTAGCTGCCTTCTCACTATAAGGCAACGCGTGCATACATCGTTGTACAAACGATTCCTTATATTTCCTTCTCAGTCAAATAAGCTAACCTGAGACTTTCCACAAACAGCAAGTCTGCAATCATCCTCCCTAAAAGCTGCATTCAGCAAACCAAATTTCATTACATTTTTATTTGTTGGCTGTAGTTATCTCGGTTATATCATCTTCACGTTGAAATGCCATTTAGATATTTTGCAGTAGGATTAGACTACCGTACCATTGTTATGAGGTTGAGTATTAATACATTTCTGGTATTTTATGCAGATGGAATTGTTTCTTTGAGAACTACTAGTAAGTTCCATTTTCTCAAAATTAAACTATTTTTAATTAATATCCTGGGGAATAATGTAATTAAGATCTTCATCGACACCTCCTAGGAAGAGTCAACAGCTACACCATAGAATGTGAGGTCCCTTCCTCTTTGTGGCCCAAGATCAAAGTGATTTAAGCCTTTCTGGATTTCATGGAATACTGAGTTTCTAGATCACTTGCTAAGTGTGCATAGTAGTATAGGCGTAGAATAAGATTAGTGCGTTACAAATAATAAATAAAAAGAAGTTAGAATGCCGTAGAAGAATATTTCACGGAATACTGAGTGTCTAGATCATTTTGAGTTGTTTTCCGCTCTCAAGTTGTACTTTGTTGTTTTAAATCTAGTACCATTTTTTAGTGCATATACTAACGTCCTTTGGAATTTTCAGATTTTTGTGATAATGAGATAAGTTGCTATCAGTTGAATCTGAAAGAAAATGTCACTATCAGGGAGCCAATTTGGCTACCTTCAAACGTTAAGCATGGTGTGAAATTCAGGCATGGAATCACTGGTACTTTTTGGTCACTTCTCATTAATTTTTTTTTATTGGAGGTAATTGGATTTTTTTTTTTTAGTGGAACCGAAGTGCGTTTGTCTGTTTCTGAAGCTGTTGATGTTTTGCTAGCGGAGATCAATTGCTTCTTTCAGAAGGTCCCTTCCCCCTTTATTTATCGCTGAACTCAATCTAGTCTTAACTTTTGGCTTAGTGAAACATTTTACTGGATCTTATCTAATCTGTTTTACTGTATTTCAATTATTATACAGCTTAAAATAATGTTCATTAGTTGTGCGTTATTAACCACAAAAGTAACACGATAGTTGGTTCAAAATATTTCAATCATGAAGACTAATGCTTTTGTCATGCTTTCTCTTCTTTGTTCCCTTTGCTACAATACTAAGGTTTTGGTTGCTGTCTGCACAAAATGAATAAGGCAATATGCACGATATGCACAATGCTTCCATATAGCCTCTTATAGATAACAAAATGAATTAATCCTGAGATGAAATATCAAGTTCCAGAGTCTTGGGTAATTCTAGACTGCTGCTCTTGTCTTCCACTCAGTCCTATCATGGACTGTTACTGTCTATAAAACACGGACACGGCAAAAAAGGAGAAGTATCTGTGTCGGACACGTGTCGGATACGGATACGTCCGGACACGCTCTTAATATTTTACAGTAACCTTCTAACTCAAACGAATTTGAAGGCCGGCCTTGACGAAATAGAAAGTGAAAGTGAAGCATTGATTCAAAAGATAGTTTTTAGATTTAGATAATGGAAGTTTATTAAGGTTTTAGGATACTCTATTGTATTCTCGGTACAAGCAACGTAGTCTCCTCTAGGTAATAAAATATTAAGAGCCAGTAGTAAGAAGTTCAGTTTTGTAATTATCCTAATTGAGATGAACCTGATTAACCATTAATGTTGATCTTTTTGAAAGTCCAGGGTAGAGCAAACTGCTTACTCTCTGTAACTTTAGACTTGTAACTTTATTTATTTACTCTCAAGTTTTATTATTTCTTATCCTAGTGATATCGAACAGCCTTTAGTATCAAATCAACCATGTAGTTTATATTTCTCAAGGTGATTGTTTCCTGAATCATCGTTACATATCTGACCCAAGTTTATCCCGCTTTATTTTGCAGATTCGCATTCTAAAGATCCCTGTAAGTGGAGCTTGTTACTCTATTCCACGCTTCAAATATTATATGCTAACAAAAATTTTCAATGAAACTCCGCAAAAGAACTGCATTTTATGGAACTGGTTTTGTCAACATAATATCTCTTATGTAATTATATATTTAGTCATGCATGGCTTGGCAAGAATTTTCATATTAGAAACCTAAATTTTGGAGAAGAATCTACACGAACAGATATATATTGCAAATTGCCTTACTATGGTGATGCATTTGGCACTAAAGGAACTCATAAGCTTTTCAATTTCATAAGAATGTTGCTATGGAGGTTGTGGTCGAGTGCCAGGATATCCCAGGGTCACGAAATGATGCAGTCTTCCTGGAAAACTTGTCCTCTAGTTCCTCTTTCACAGCCTCAACCCTGGATCATCTGAAATTGGGCCTTGAGGATTATGTATCAAGGCATGGATCTAGTTTGACGTGTGACTCTTGCTTCCCTAACAGGTGTGTTTGTCACCTTAATTCATGCAAACTCAGTTTTTAGACTCAACTTCCTTTGTTTTTAGGTTATTGTAAAAAGAAGAAATAATTTCCTGATGAGATATAAAGCAAGTTTGTTCCACATTTTTCCTGGATTAAGTGAAGCTTTCCCAATTTCGCACTCAGATTTTAATAATTAATTGCTGAACTTGCCTTTGATGCGCATTATATATATACTTCTTTAATTTCGAACTTTAGGGATAATCTGAAGAGTGGGGGTGGAATGATTTGTGAAGAAAAGCGTAAAACGACCACACTGGCAGTGGAGGCAGCGATTGTGATAAGTGAATTATCAAATCCGACCACTAATTGCTTGGGAGCACGGTTCTCCGATACAGAGGTTGTTTGACATATTAAGTATTATCATTCGGTTCCTTCCTGTTCTTTCCGTTCCAGAAAGTAGCAAGCAAGTTTTCCAAATACGAACTATATTCCGAAGCATTCAGACCACATCTCTGTACTACAATCAAAGAGTGCTATCTGTTTCAGGAACTTATTTTTATTAGTCTTCCTTTTCTCTTGGTGATCGCAACTTTGCCTTAGCTTTTACTATGTCACATGATTCCGCAGGTTTTTTGTTTTAAAGATTTTGCACCTTGCTCGATCTCTGAGGCATTTCTAAAGGCATTAAGAGGCATTGACTGGAAACGTTATGGTTTGTCTTTGGAAAGTGCTACCAATCAAAGAAGCCATGCATTACTAAAGTGGGAACACGTGCCCCTATCTTTTCATATTCATATTGTTGTCCACTGCTACCAGAAGGTATATTATTCATTAGATCTTATGTTTAAAGGTTAACAATGATTATTGTGATCTTTATTTTCTATGGTATGTAAAGTTAGTTTTCTGCCTTAGCTTGTGGCCGAGGTCATGCCATTGGTGCAGAAGACTCAGTTTGATAAAAAACTCATAAGTAAAGCAGTTAAGCTTGCACTGGACGATTTGAAGAATAAATATGCGGGATTTCTTCTCAGTGCCAATACCCTGAAGGTAAACGTATGTTTCACAGTCTCGGGTGCACAGACTAAATTGATCATTAAGTTCTAAGTTTCAAAATGGAAGTCCTTTTTTGTGGCAAACAATAAAAAAATTCTTCCATCACGCATTTTTTATTATCCCACACAGTCACGAAGCCAAACACAGTTTAGTGAAGAATTCTGCTAAGCTTTCAGGTTTGGTAACCAATTTTTGACTATAGTGAATTCTTTTGGAAATGCTAGCAATACAATGTAGTTTCTTTTGTATGTGTAATTGTTTGCAGCAATTAAATATAACTTTTTCTAAAATCGTATCTTGGTATTTTTTGTTTTTGCTTGTTTCTTCTGGTTTTTAAAAACAAAAAGAACATATTTGGTATTTGCCATTGAAGTTGGATCAAATTAATCACACTCATGTCATCCTATCTCACAAAAAGAGAAAAAGGATTAGAGCTTGCTTTCTTATAATTTTAGGTTTCCATGTTCAGGGTATTTACATATTATTCATGAAACTGTTTAGGCTTCTCGTAGGAATCAAGTGAAGTTGACAGCTTCCTGAACTTGCCTGTATTATTCATCTTCAGATCAGTAGGTTTGCCCCTGATCTTGCCAAAACGATTGCTGGCCTAGTTTTATACTCCAATGACTTGGATTTCCAAGAAGAATGCCTTGCAATTCTTGGCTTGCAACCTCAACAATCAGAAGGGGAAATTGTTGAAGAAAATATTAAGGAAAGGATAATTTCAGTCATTGAGGTGAATGACGGAAGGCCAAAAGGAACAAAAGAAGTTGCCCCTCTCTTGTTTGGAGATGGTCGCCACCAACTACAGTTTGTGGACGATGAATGTGATGAAGATGGATTTGATCCAATGGAGTTGTAA

mRNA sequence

ATGACAAAAACAAGTATATCCATCGCCCAGAATGAGAGGAACGATGTTCTAGAAGCATTTAATCCTGCAATATCCAATTTCCTTGCGCAGCTTTCTCAGAAAGTTCAAAATTCTCTGAAGCCACAGCTTAATAGATTGGCAAATGAAGGTCAGAAATTGACGTCAATGAGTCCATTCGCAAAAGGGGAGAGGAAGGGATCTTCCACATCATCCTTGAAGCTTGATGTGGAGAAGCAACTGCAGGCATGGAGAGAGAACCCTTCATGGACTGACAAACCTCCACAAATAAAGGTAACTGTACCGAAAGATACCCTTAGCAGACTCAATGCAAAGGTTGATGTTGGTTTGCCACCAGATGCTGTCTATAATATCGTGACAGACCCTGATAACAAGAGAGTTTTCAAGAATATTAAAGAGGTGATATCCAGAAAGGTTTTGATTGATGAAGGCTCGAGACAAGTGGTTGAGGTGGAGCAAGCAGCTTTGTGGAGATTTCTTTGGTGGTCCGGGACCATTTCAGTTCATGTTCTAGTAGATCAAAACAGAGCGGATCACTCAATGAAGTTCAAGCAACTGAAGACTGGATTTATGAAGAAATTCGAAGGCTGCTGGAGAGTAGAGCCTATATTTGTTGATGAAAGCGTCTGCTTTCCAGTAAAACCCAAGAATTTGACAGATTATCATGCTTGTACAAAGGGCAAGGGAAGAATAGGGTCAAGGGTGAGCCTCGAACAGCTGATCCAGCCAGCCATTGTTCCACCACCGCCCATCTCCTGGTATCTTCGCGGAATTACCACCAGGACCACTGAGATGCTCATTCTTGATCTGCTTGCTGAAGCCAAAAGGATAAGGGAAGATGCCAAAGGTCAAACTTTGAACAATGAGCTTGATATTTCTCAGGGAACGTGTGATAGCAACTTATTAGATAGCATTCTTGATATTAAAGAAAGATGGGCAATGCATAGGAGGAATGCGAAGCCATGTCGAGCGCGGGAGTCTGTTTCTAGTCTTCTCCCAAAACTTCTACCATGGAGTTTAATTCGCTTCAAGACCTCTTCCTTCTTCAATACGGAGATGCATCTGCTCCGAACGACTTTGCAGACTCTCAGTTCTTCTGCAACGCTCTGCCGCTTCCGATCCTCCCCTATTTCAGATACCGGCCTTGGAAGCTGCCTAGAGGAGTTTCAGGATTTTAAGTGTCCCGTTGAAGGTATCCTTGCTGAACAGTGGGTAGCTCATGTACAGAGATATTTTGCAGTAGGATTAGACTACCGTACCATTGTTATGAGGTTGAGTATTAATACATTTCTGGTATTTTATGCAGATGGAATTGTTTCTTTGAGAACTACTAATTTTTGTGATAATGAGATAAGTTGCTATCAGTTGAATCTGAAAGAAAATGTCACTATCAGGGAGCCAATTTGGCTACCTTCAAACGTTAAGCATGGTGTGAAATTCAGGCATGGAATCACTGCTGTTGATGTTTTGCTAGCGGAGATCAATTGCTTCTTTCAGAAGATTCGCATTCTAAAGATCCCTAATGTTGCTATGGAGGTTGTGGTCGAGTGCCAGGATATCCCAGGGTCACGAAATGATGCAGTCTTCCTGGAAAACTTGTCCTCTAGTTCCTCTTTCACAGCCTCAACCCTGGATCATCTGAAATTGGGCCTTGAGGATTATGTATCAAGGCATGGATCTAGTTTGACGTGTGACTCTTGCTTCCCTAACAGGGATAATCTGAAGAGTGGGGGTGGAATGATTTGTGAAGAAAAGCGTAAAACGACCACACTGGCAGTGGAGGCAGCGATTGTGATAAGTGAATTATCAAATCCGACCACTAATTGCTTGGGAGCACGGTTCTCCGATACAGAGGTTTTTTGTTTTAAAGATTTTGCACCTTGCTCGATCTCTGAGGCATTTCTAAAGGCATTAAGAGGCATTGACTGGAAACGTTATGGTTTGTCTTTGGAAAGTGCTACCAATCAAAGAAGCCATGCATTACTAAAGTGGGAACACGTGCCCCTATCTTTTCATATTCATATTGTTGTCCACTGCTACCAGAAGCTTGTGGCCGAGGTCATGCCATTGGTGCAGAAGACTCAGTTTGATAAAAAACTCATAAGTAAAGCAGTTAAGCTTGCACTGGACGATTTGAAGAATAAATATGCGGGATTTCTTCTCAGTGCCAATACCCTGAAGATCAGTAGGTTTGCCCCTGATCTTGCCAAAACGATTGCTGGCCTAGTTTTATACTCCAATGACTTGGATTTCCAAGAAGAATGCCTTGCAATTCTTGGCTTGCAACCTCAACAATCAGAAGGGGAAATTGTTGAAGAAAATATTAAGGAAAGGATAATTTCAGTCATTGAGGTGAATGACGGAAGGCCAAAAGGAACAAAAGAAGTTGCCCCTCTCTTGTTTGGAGATGGTCGCCACCAACTACAGTTTGTGGACGATGAATGTGATGAAGATGGATTTGATCCAATGGAGTTGTAA

Coding sequence (CDS)

ATGACAAAAACAAGTATATCCATCGCCCAGAATGAGAGGAACGATGTTCTAGAAGCATTTAATCCTGCAATATCCAATTTCCTTGCGCAGCTTTCTCAGAAAGTTCAAAATTCTCTGAAGCCACAGCTTAATAGATTGGCAAATGAAGGTCAGAAATTGACGTCAATGAGTCCATTCGCAAAAGGGGAGAGGAAGGGATCTTCCACATCATCCTTGAAGCTTGATGTGGAGAAGCAACTGCAGGCATGGAGAGAGAACCCTTCATGGACTGACAAACCTCCACAAATAAAGGTAACTGTACCGAAAGATACCCTTAGCAGACTCAATGCAAAGGTTGATGTTGGTTTGCCACCAGATGCTGTCTATAATATCGTGACAGACCCTGATAACAAGAGAGTTTTCAAGAATATTAAAGAGGTGATATCCAGAAAGGTTTTGATTGATGAAGGCTCGAGACAAGTGGTTGAGGTGGAGCAAGCAGCTTTGTGGAGATTTCTTTGGTGGTCCGGGACCATTTCAGTTCATGTTCTAGTAGATCAAAACAGAGCGGATCACTCAATGAAGTTCAAGCAACTGAAGACTGGATTTATGAAGAAATTCGAAGGCTGCTGGAGAGTAGAGCCTATATTTGTTGATGAAAGCGTCTGCTTTCCAGTAAAACCCAAGAATTTGACAGATTATCATGCTTGTACAAAGGGCAAGGGAAGAATAGGGTCAAGGGTGAGCCTCGAACAGCTGATCCAGCCAGCCATTGTTCCACCACCGCCCATCTCCTGGTATCTTCGCGGAATTACCACCAGGACCACTGAGATGCTCATTCTTGATCTGCTTGCTGAAGCCAAAAGGATAAGGGAAGATGCCAAAGGTCAAACTTTGAACAATGAGCTTGATATTTCTCAGGGAACGTGTGATAGCAACTTATTAGATAGCATTCTTGATATTAAAGAAAGATGGGCAATGCATAGGAGGAATGCGAAGCCATGTCGAGCGCGGGAGTCTGTTTCTAGTCTTCTCCCAAAACTTCTACCATGGAGTTTAATTCGCTTCAAGACCTCTTCCTTCTTCAATACGGAGATGCATCTGCTCCGAACGACTTTGCAGACTCTCAGTTCTTCTGCAACGCTCTGCCGCTTCCGATCCTCCCCTATTTCAGATACCGGCCTTGGAAGCTGCCTAGAGGAGTTTCAGGATTTTAAGTGTCCCGTTGAAGGTATCCTTGCTGAACAGTGGGTAGCTCATGTACAGAGATATTTTGCAGTAGGATTAGACTACCGTACCATTGTTATGAGGTTGAGTATTAATACATTTCTGGTATTTTATGCAGATGGAATTGTTTCTTTGAGAACTACTAATTTTTGTGATAATGAGATAAGTTGCTATCAGTTGAATCTGAAAGAAAATGTCACTATCAGGGAGCCAATTTGGCTACCTTCAAACGTTAAGCATGGTGTGAAATTCAGGCATGGAATCACTGCTGTTGATGTTTTGCTAGCGGAGATCAATTGCTTCTTTCAGAAGATTCGCATTCTAAAGATCCCTAATGTTGCTATGGAGGTTGTGGTCGAGTGCCAGGATATCCCAGGGTCACGAAATGATGCAGTCTTCCTGGAAAACTTGTCCTCTAGTTCCTCTTTCACAGCCTCAACCCTGGATCATCTGAAATTGGGCCTTGAGGATTATGTATCAAGGCATGGATCTAGTTTGACGTGTGACTCTTGCTTCCCTAACAGGGATAATCTGAAGAGTGGGGGTGGAATGATTTGTGAAGAAAAGCGTAAAACGACCACACTGGCAGTGGAGGCAGCGATTGTGATAAGTGAATTATCAAATCCGACCACTAATTGCTTGGGAGCACGGTTCTCCGATACAGAGGTTTTTTGTTTTAAAGATTTTGCACCTTGCTCGATCTCTGAGGCATTTCTAAAGGCATTAAGAGGCATTGACTGGAAACGTTATGGTTTGTCTTTGGAAAGTGCTACCAATCAAAGAAGCCATGCATTACTAAAGTGGGAACACGTGCCCCTATCTTTTCATATTCATATTGTTGTCCACTGCTACCAGAAGCTTGTGGCCGAGGTCATGCCATTGGTGCAGAAGACTCAGTTTGATAAAAAACTCATAAGTAAAGCAGTTAAGCTTGCACTGGACGATTTGAAGAATAAATATGCGGGATTTCTTCTCAGTGCCAATACCCTGAAGATCAGTAGGTTTGCCCCTGATCTTGCCAAAACGATTGCTGGCCTAGTTTTATACTCCAATGACTTGGATTTCCAAGAAGAATGCCTTGCAATTCTTGGCTTGCAACCTCAACAATCAGAAGGGGAAATTGTTGAAGAAAATATTAAGGAAAGGATAATTTCAGTCATTGAGGTGAATGACGGAAGGCCAAAAGGAACAAAAGAAGTTGCCCCTCTCTTGTTTGGAGATGGTCGCCACCAACTACAGTTTGTGGACGATGAATGTGATGAAGATGGATTTGATCCAATGGAGTTGTAA

Protein sequence

MTKTSISIAQNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFAKGERKGSSTSSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDAVYNIVTDPDNKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQNRADHSMKFKQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSRVSLEQLIQPAIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQGTCDSNLLDSILDIKERWAMHRRNAKPCRARESVSSLLPKLLPWSLIRFKTSSFFNTEMHLLRTTLQTLSSSATLCRFRSSPISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYADGIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFRHGITAVDVLLAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLGLEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCLGARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLSFHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISRFAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRPKGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL
Homology
BLAST of Moc08g00200 vs. NCBI nr
Match: XP_022131879.1 (type 2 DNA topoisomerase 6 subunit B-like isoform X1 [Momordica charantia])

HSP 1 Score: 807.0 bits (2083), Expect = 1.5e-229
Identity = 414/455 (90.99%), Postives = 416/455 (91.43%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           ISDTGLGSCLEEFQDFKCPVEGILAEQW                               D
Sbjct: 49  ISDTGLGSCLEEFQDFKCPVEGILAEQW-------------------------------D 108

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF-----RHGIT-AVDVL 502
           GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF     R  ++ AVDVL
Sbjct: 109 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFSGTEVRLSVSEAVDVL 168

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG
Sbjct: 169 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 228

Query: 563 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 622
           LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL
Sbjct: 229 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 288

Query: 623 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 682
           GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS
Sbjct: 289 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 348

Query: 683 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 742
           FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR
Sbjct: 349 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 408

Query: 743 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 802
           FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP
Sbjct: 409 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 468

Query: 803 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 832
           KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL
Sbjct: 469 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 472

BLAST of Moc08g00200 vs. NCBI nr
Match: XP_022131880.1 (type 2 DNA topoisomerase 6 subunit B-like isoform X2 [Momordica charantia])

HSP 1 Score: 805.1 bits (2078), Expect = 5.8e-229
Identity = 413/455 (90.77%), Postives = 416/455 (91.43%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           IS+TGLGSCLEEFQDFKCPVEGILAEQW                               D
Sbjct: 47  ISNTGLGSCLEEFQDFKCPVEGILAEQW-------------------------------D 106

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF-----RHGIT-AVDVL 502
           GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF     R  ++ AVDVL
Sbjct: 107 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFSGTEVRLSVSEAVDVL 166

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG
Sbjct: 167 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 226

Query: 563 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 622
           LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL
Sbjct: 227 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 286

Query: 623 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 682
           GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS
Sbjct: 287 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 346

Query: 683 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 742
           FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR
Sbjct: 347 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 406

Query: 743 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 802
           FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP
Sbjct: 407 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 466

Query: 803 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 832
           KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL
Sbjct: 467 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 470

BLAST of Moc08g00200 vs. NCBI nr
Match: KAG7029197.1 (Type 2 DNA topoisomerase 6 subunit B-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 678.3 bits (1749), Expect = 8.2e-191
Identity = 350/484 (72.31%), Postives = 391/484 (80.79%), Query Frame = 0

Query: 351 TSSFFNTEMHLLRTTLQTLSSSATLCRFRSSPISDTGLGSCLEEFQDFKCPVEGILAEQW 410
           T+ F NTEMHLL TTLQTLSSSATL RFRSS ISDTGLGSC+EEFQ+ KCPVEGILAE W
Sbjct: 33  TNFFGNTEMHLLGTTLQTLSSSATLSRFRSSTISDTGLGSCIEEFQNLKCPVEGILAETW 92

Query: 411 VAHVQRYFAVGLDYRTIVMRLSINTFLVFYADGIVSLRTT---NFCDNEISCYQLNLKEN 470
                                          +GIV +RTT   + CD EISCY+LNLKEN
Sbjct: 93  -------------------------------NGIVYVRTTKIADICDKEISCYELNLKEN 152

Query: 471 VTIREPIWLPSNVKHGVKFRHGITAVDVLLAEINCFFQKIRILKIPNVAMEVVVECQDIP 530
           VT R+PI LPSN+KH         +VDVLL E NCFFQKI +LK+PN+AMEV+ + QD+P
Sbjct: 153 VTTRKPILLPSNIKH--VCLSVSESVDVLLEEFNCFFQKILVLKVPNIAMEVMADGQDVP 212

Query: 531 GSRNDAVFLENLSSSSSFTASTLDHLKLGLEDYVSRHGSSLTCDSCFPNRDNLKSGGGMI 590
           GSRNDAVFL+  S SSSF ASTLDHLKLGLE YV RHGSSL CDSCFPNRDNL+SGGGM+
Sbjct: 213 GSRNDAVFLQKFSPSSSFAASTLDHLKLGLESYVLRHGSSLACDSCFPNRDNLRSGGGMV 272

Query: 591 CEEKRKTTTLAVEAAIVISELSNPTTNCLGARFSDTEVFCFKDFAPCSISEAFLKALRGI 650
           CE+K KTT L VEAA+V SE SNP+ NC GA  SDT+V CFKDFAPCSISEA LKAL GI
Sbjct: 273 CEDKHKTTKLVVEAAVVTSESSNPSNNCFGAGCSDTKVLCFKDFAPCSISEALLKALTGI 332

Query: 651 DWKRYGLSLESATNQRSHALLKWEHVPLSFHIHIVVHCYQKLVAEVMPLVQKTQFDKKLI 710
           DWKRYGL+LE A +QRSHALLKWEH+PLSFHIHIVVHCY KLV E MPL+QKT+FDKKLI
Sbjct: 333 DWKRYGLALECAIDQRSHALLKWEHMPLSFHIHIVVHCYDKLVFEAMPLLQKTRFDKKLI 392

Query: 711 SKAVKLALDDLKNKYAGFLLSANTLKISRFAPDLAKTIAGLVLYSNDLDFQEECLAILGL 770
            KAVKLALDD KNK+AG LLSA+TLKIS+FAPDLAK+IAGLVLYS+D+DF+EECLAILGL
Sbjct: 393 RKAVKLALDDFKNKHAGVLLSADTLKISKFAPDLAKSIAGLVLYSSDMDFKEECLAILGL 452

Query: 771 QPQQSEGEIVEENIKERIISVIEVNDGRPKGTKEVAPLLFGDGRHQLQFVDDECDEDGFD 830
           QP QSEGEIV ENIK++IIS IEVND R +GTKEVAPLLF +GRH+LQFVDDECDEDGFD
Sbjct: 453 QPHQSEGEIVGENIKKKIISAIEVNDRRQQGTKEVAPLLF-EGRHELQFVDDECDEDGFD 482

Query: 831 PMEL 832
           PM+L
Sbjct: 513 PMDL 482

BLAST of Moc08g00200 vs. NCBI nr
Match: XP_022132255.1 (uncharacterized protein LOC111005155 [Momordica charantia] >XP_022132256.1 uncharacterized protein LOC111005155 [Momordica charantia])

HSP 1 Score: 664.8 bits (1714), Expect = 9.4e-187
Identity = 330/331 (99.70%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MTKTSISIAQNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFA 60
           MTKTSISIAQNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFA
Sbjct: 1   MTKTSISIAQNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFA 60

Query: 61  KGERKGSSTSSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDA 120
           KGERKGSSTSSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDA
Sbjct: 61  KGERKGSSTSSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDA 120

Query: 121 VYNIVTDPDNKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQ 180
           VYNIVTDPDNKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQ
Sbjct: 121 VYNIVTDPDNKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQ 180

Query: 181 NRADHSMKFKQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSR 240
           NRADHSMKFKQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSR
Sbjct: 181 NRADHSMKFKQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSR 240

Query: 241 VSLEQLIQPAIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQ 300
           VSLEQLIQPAIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQ
Sbjct: 241 VSLEQLIQPAIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQ 300

Query: 301 GTCDSNLLDSILDIKERWAMHRRNAKPCRAR 332
           GTCDSNLLDSILDIKERWAMHRRNAKPCR R
Sbjct: 301 GTCDSNLLDSILDIKERWAMHRRNAKPCRRR 331

BLAST of Moc08g00200 vs. NCBI nr
Match: XP_023545219.1 (type 2 DNA topoisomerase 6 subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.9 bits (1696), Expect = 1.2e-184
Identity = 331/455 (72.75%), Postives = 371/455 (81.54%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           ISDTGLGSC+EEFQ+ KCPVEGILAE W                               +
Sbjct: 49  ISDTGLGSCIEEFQNLKCPVEGILAETW-------------------------------N 108

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFR------HGITAVDVL 502
           GIV +RTTN CD EISCY+LNLKENVT R+PI LPSN+KHGVKF           +VDVL
Sbjct: 109 GIVYVRTTNICDKEISCYELNLKENVTTRKPILLPSNIKHGVKFSGTEVCLSVSESVDVL 168

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           L E NCFFQKI +LK+PN+AMEV+ + QD+PGSRNDAVFLE +S+SSSF ASTLDHLKLG
Sbjct: 169 LEEFNCFFQKILVLKVPNIAMEVMADGQDVPGSRNDAVFLEKISTSSSFAASTLDHLKLG 228

Query: 563 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 622
           LE YV RHGSSL CDSCFPNRDNL+SGGGM+CE+K KTT L VEAA+V SE+SNPT NC 
Sbjct: 229 LESYVLRHGSSLACDSCFPNRDNLRSGGGMVCEDKHKTTKLVVEAAVVTSEISNPTNNCF 288

Query: 623 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 682
           GA  SDT+V CFKDFAPCSISEA LKAL GIDWKRYGL+LE A +QRSHALLKWEH+PLS
Sbjct: 289 GAGCSDTKVLCFKDFAPCSISEALLKALTGIDWKRYGLALECAIDQRSHALLKWEHMPLS 348

Query: 683 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 742
           FHIHIVVHCY KLV E MPL+QKT+FDKKLI KAVKLALDD KNK+AG LLSA+TLKIS+
Sbjct: 349 FHIHIVVHCYDKLVFEAMPLLQKTRFDKKLIRKAVKLALDDFKNKHAGVLLSADTLKISK 408

Query: 743 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 802
           FAPDLAK+IAGLVLYS+D+DF+EECLAILGLQP QSEGEIV ENIK++IIS IEVND R 
Sbjct: 409 FAPDLAKSIAGLVLYSSDMDFKEECLAILGLQPHQSEGEIVGENIKKKIISAIEVNDRRQ 468

Query: 803 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 832
           +GTKEVAPLLF +GRH+LQFVDDECDEDGFDPM+L
Sbjct: 469 QGTKEVAPLLF-EGRHELQFVDDECDEDGFDPMDL 471

BLAST of Moc08g00200 vs. ExPASy Swiss-Prot
Match: Q5Q0E6 (Type 2 DNA topoisomerase 6 subunit B-like OS=Arabidopsis thaliana OX=3702 GN=MTOPVIB PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 6.8e-79
Identity = 174/447 (38.93%), Postives = 250/447 (55.93%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           I+DTG+G  LEEFQ+ +CP E   A+ W                               D
Sbjct: 50  IADTGIGCNLEEFQNLRCPREFNGAKIW-------------------------------D 109

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFR------HGITAVDVL 502
           G++S++TT F D+E+  Y +NL E +  +     PS  K+G KF           ++DVL
Sbjct: 110 GLLSVKTTCFTDDEVYYYHINLDEYIANKRLKRQPSQAKNGAKFSGTEVSLSVFGSMDVL 169

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           +A I  FFQKI +L+I NV ++++V+    PG++   VF  N   +  FTAS L+ LK G
Sbjct: 170 VAPIIGFFQKIIVLQILNVTLDLMVKQGTSPGNQTQYVFAVNADKTPCFTASNLERLKSG 229

Query: 563 LEDYVSRHGSSL--TCDSCFPNRDNLKSGGGMIC-EEKRKTTTLAVEAAIVISELSNPTT 622
           LEDYV RH + L   CD CF +R++LK G G +C E+K K     +E  IVIS+L   T 
Sbjct: 230 LEDYVLRHANCLDTMCDYCFSDREHLKVGSGTVCQEDKHKRVGGTMEVVIVISDLLESTQ 289

Query: 623 NCLGARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHV 682
           +C  +    TEV  F +F P  +    L AL+ IDWK+YGL L +  +Q  H  L+W++ 
Sbjct: 290 HCSRSCNGKTEVLYFDNFLPSPVPHLALSALKKIDWKKYGLILANVNDQDGHVFLEWDNF 349

Query: 683 PLSFHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLK 742
           P    I I +H Y        P  QK      L+ K +K ALD+LK K+ GFLLS+++ K
Sbjct: 350 PSYVQIQIALHWYH----NQYPTRQKNGPGISLLKKGIKNALDNLKAKHEGFLLSSHSRK 409

Query: 743 ISRFAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVND 802
           I  + PDLA++IAGL+  S DLDFQ +CL++LG Q Q+ E + VE  I+ +I++VI +N+
Sbjct: 410 ICSYVPDLARSIAGLIFSSTDLDFQGDCLSVLGFQTQEVERDTVENYIQRKIVTVIGMNE 461

Query: 803 GRPKGTKEVAPLLFGDGRHQLQFVDDE 821
            +P+  +E AP LF DG  +  F +DE
Sbjct: 470 RKPQKDQEAAPFLFFDGESETSFFEDE 461

BLAST of Moc08g00200 vs. ExPASy TrEMBL
Match: A0A6J1BRH4 (type 2 DNA topoisomerase 6 subunit B-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004913 PE=4 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 7.4e-230
Identity = 414/455 (90.99%), Postives = 416/455 (91.43%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           ISDTGLGSCLEEFQDFKCPVEGILAEQW                               D
Sbjct: 49  ISDTGLGSCLEEFQDFKCPVEGILAEQW-------------------------------D 108

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF-----RHGIT-AVDVL 502
           GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF     R  ++ AVDVL
Sbjct: 109 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFSGTEVRLSVSEAVDVL 168

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG
Sbjct: 169 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 228

Query: 563 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 622
           LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL
Sbjct: 229 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 288

Query: 623 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 682
           GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS
Sbjct: 289 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 348

Query: 683 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 742
           FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR
Sbjct: 349 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 408

Query: 743 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 802
           FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP
Sbjct: 409 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 468

Query: 803 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 832
           KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL
Sbjct: 469 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 472

BLAST of Moc08g00200 vs. ExPASy TrEMBL
Match: A0A6J1BQX4 (type 2 DNA topoisomerase 6 subunit B-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004913 PE=4 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 2.8e-229
Identity = 413/455 (90.77%), Postives = 416/455 (91.43%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           IS+TGLGSCLEEFQDFKCPVEGILAEQW                               D
Sbjct: 47  ISNTGLGSCLEEFQDFKCPVEGILAEQW-------------------------------D 106

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF-----RHGIT-AVDVL 502
           GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKF     R  ++ AVDVL
Sbjct: 107 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFSGTEVRLSVSEAVDVL 166

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG
Sbjct: 167 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 226

Query: 563 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 622
           LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL
Sbjct: 227 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 286

Query: 623 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 682
           GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS
Sbjct: 287 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 346

Query: 683 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 742
           FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR
Sbjct: 347 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 406

Query: 743 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 802
           FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP
Sbjct: 407 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 466

Query: 803 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 832
           KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL
Sbjct: 467 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 470

BLAST of Moc08g00200 vs. ExPASy TrEMBL
Match: A0A6J1BRY3 (uncharacterized protein LOC111005155 OS=Momordica charantia OX=3673 GN=LOC111005155 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 4.6e-187
Identity = 330/331 (99.70%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MTKTSISIAQNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFA 60
           MTKTSISIAQNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFA
Sbjct: 1   MTKTSISIAQNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFA 60

Query: 61  KGERKGSSTSSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDA 120
           KGERKGSSTSSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDA
Sbjct: 61  KGERKGSSTSSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDA 120

Query: 121 VYNIVTDPDNKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQ 180
           VYNIVTDPDNKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQ
Sbjct: 121 VYNIVTDPDNKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQ 180

Query: 181 NRADHSMKFKQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSR 240
           NRADHSMKFKQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSR
Sbjct: 181 NRADHSMKFKQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSR 240

Query: 241 VSLEQLIQPAIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQ 300
           VSLEQLIQPAIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQ
Sbjct: 241 VSLEQLIQPAIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQ 300

Query: 301 GTCDSNLLDSILDIKERWAMHRRNAKPCRAR 332
           GTCDSNLLDSILDIKERWAMHRRNAKPCR R
Sbjct: 301 GTCDSNLLDSILDIKERWAMHRRNAKPCRRR 331

BLAST of Moc08g00200 vs. ExPASy TrEMBL
Match: A0A6J1HCF2 (type 2 DNA topoisomerase 6 subunit B-like isoform X6 OS=Cucurbita moschata OX=3662 GN=LOC111462807 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 3.1e-183
Identity = 328/455 (72.09%), Postives = 368/455 (80.88%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           ISDTGLGSC+EEFQ+ KCPVEGILAE W                               +
Sbjct: 49  ISDTGLGSCIEEFQNLKCPVEGILAETW-------------------------------N 108

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFR------HGITAVDVL 502
           GIV +RTTN CD EISCY+LNLKENVT R+PI LPSN+KHGVKF           +VDVL
Sbjct: 109 GIVYVRTTNICDKEISCYELNLKENVTTRKPILLPSNIKHGVKFSGTEVCLSVSESVDVL 168

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           L E NCFFQKI +LK+PN+AMEV+ + QD+PGSRNDAVFLE  S SSSF ASTLDHLKLG
Sbjct: 169 LEEFNCFFQKILVLKVPNIAMEVMADGQDVPGSRNDAVFLEKFSPSSSFAASTLDHLKLG 228

Query: 563 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 622
           LE YV RHGSSL CDSCFPNRDNL+SGGGM+CE+K KTT L VEAA+V SE+ NPT NC 
Sbjct: 229 LESYVLRHGSSLACDSCFPNRDNLRSGGGMVCEDKHKTTKLVVEAAVVTSEIPNPTNNCF 288

Query: 623 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 682
           GA  SDT+V CFKDFAPCSISEA LKAL GIDWKRYGL+LE A +QRSHALLKWEH+PLS
Sbjct: 289 GAGCSDTKVLCFKDFAPCSISEALLKALTGIDWKRYGLALECAIDQRSHALLKWEHMPLS 348

Query: 683 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 742
           FHIHIVVHCY KLV E MPL+QKT+FDKKLI KAVKLALDD KNK+AG LLSA+TLKIS+
Sbjct: 349 FHIHIVVHCYDKLVFEAMPLLQKTRFDKKLIRKAVKLALDDFKNKHAGVLLSADTLKISK 408

Query: 743 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 802
           FAPDLAK+IAGLVLYS+D+DF+EECLAILGLQP QSEGEIV ENIK++IIS IEVND R 
Sbjct: 409 FAPDLAKSIAGLVLYSSDMDFKEECLAILGLQPHQSEGEIVGENIKKKIISAIEVNDRRQ 468

Query: 803 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 832
           +GT+EVAPLLF +GRH+LQ+VDDECDEDGFDPM+L
Sbjct: 469 QGTREVAPLLF-EGRHELQYVDDECDEDGFDPMDL 471

BLAST of Moc08g00200 vs. ExPASy TrEMBL
Match: A0A6J1K810 (type 2 DNA topoisomerase 6 subunit B-like isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111491995 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 8.3e-181
Identity = 325/455 (71.43%), Postives = 364/455 (80.00%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           ISDTGLGSC+EEFQ+ KCPVEGILAE W                               +
Sbjct: 49  ISDTGLGSCIEEFQNLKCPVEGILAETW-------------------------------N 108

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFR------HGITAVDVL 502
           GIV +RTTN CD EISCYQLNLKENVT R+PI LPSN+KHGVKF           ++DVL
Sbjct: 109 GIVYVRTTNICDKEISCYQLNLKENVTTRKPILLPSNIKHGVKFSGTEVCLSVSESIDVL 168

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           L E NCFFQKI +LK+PN+AMEV+ + QD+PGSRNDAVFLE  S SSSF ASTLDHLK G
Sbjct: 169 LEEFNCFFQKILVLKVPNIAMEVMADGQDVPGSRNDAVFLEKFSPSSSFAASTLDHLKFG 228

Query: 563 LEDYVSRHGSSLTCDSCFPNRDNLKSGGGMICEEKRKTTTLAVEAAIVISELSNPTTNCL 622
           LE YV RHGSSL CDSCFPNRDNL+SGGGM+CE+K KTT L VEAA+V +E+SNPT NC 
Sbjct: 229 LESYVLRHGSSLACDSCFPNRDNLRSGGGMVCEDKHKTTKLVVEAAVVTTEISNPTNNCF 288

Query: 623 GARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHVPLS 682
            A  S+T+V CFKDFAPCSISEA LKAL GIDWKRYGL+LE A +QRSHALLKWEH+PLS
Sbjct: 289 RAGCSNTKVLCFKDFAPCSISEALLKALTGIDWKRYGLALECAIDQRSHALLKWEHMPLS 348

Query: 683 FHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLKISR 742
           FHIHIVVHCY K V E MPL+QKT+FDKKLI KAVKLALDD KNK+AG LLSA+TLKIS+
Sbjct: 349 FHIHIVVHCYDKHVFEAMPLLQKTRFDKKLIRKAVKLALDDFKNKHAGVLLSADTLKISK 408

Query: 743 FAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVNDGRP 802
           FAPDLAK+IAGLVLYS+D+DF+EECLAILGLQP QSEGEIV ENIK +IIS IEVND R 
Sbjct: 409 FAPDLAKSIAGLVLYSSDMDFKEECLAILGLQPHQSEGEIVGENIKNKIISAIEVNDRRQ 468

Query: 803 KGTKEVAPLLFGDGRHQLQFVDDECDEDGFDPMEL 832
           +GTKEVAPLLF +GRH+LQFVDDECDEDG DPM+L
Sbjct: 469 QGTKEVAPLLF-EGRHELQFVDDECDEDGLDPMDL 471

BLAST of Moc08g00200 vs. TAIR 10
Match: AT1G70480.1 (Domain of unknown function (DUF220) )

HSP 1 Score: 383.6 bits (984), Expect = 3.9e-106
Identity = 195/317 (61.51%), Postives = 244/317 (76.97%), Query Frame = 0

Query: 10  QNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFAKGERKGSST 69
           +N+ N V   F+PA+S F+   SQK+Q  LK QL  L ++   + S    +  + KG+S+
Sbjct: 10  RNKGNVVSSVFDPALSRFVG-FSQKLQTRLKSQLKNLTDDKHGIGS----SWRQDKGNSS 69

Query: 70  SSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDAVYNIVTDPD 129
            S+++D+EKQL  WRENPSWTD+ P +KV +PK +L  L A+V+VGLPPDAVYNIV DPD
Sbjct: 70  ISMQIDLEKQLDCWRENPSWTDQIPVVKVGIPKGSLCNLKAEVNVGLPPDAVYNIVIDPD 129

Query: 130 NKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQNRADHSMKF 189
           N+RVFKNIKEV+SRKVL+D+G RQVVEVEQAALWRFLWWSGTISVHVLVDQ+RADHSMKF
Sbjct: 130 NRRVFKNIKEVMSRKVLVDDGLRQVVEVEQAALWRFLWWSGTISVHVLVDQDRADHSMKF 189

Query: 190 KQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSRVSLEQLIQP 249
           KQ+K+GFMK+FEG W+V+P+FVDE +C  +KPK L +Y+ CT GKGRIGS+V+L+QLIQP
Sbjct: 190 KQVKSGFMKRFEGSWQVKPLFVDEHMCDRLKPKTLEEYNRCTGGKGRIGSKVTLDQLIQP 249

Query: 250 AIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQGTCDSNLLD 309
           AIVPPPPISWYLRGIT +TTEMLI DLLAE  RIR  A G   +        + D   + 
Sbjct: 250 AIVPPPPISWYLRGITAKTTEMLIHDLLAETARIR--AAGVMEDGH------SPDEQRIG 309

Query: 310 SILDIKERWAMHRRNAK 327
           +  DIKERW+ HRR ++
Sbjct: 310 TPGDIKERWSAHRRTSR 313

BLAST of Moc08g00200 vs. TAIR 10
Match: AT1G70480.2 (Domain of unknown function (DUF220) )

HSP 1 Score: 383.6 bits (984), Expect = 3.9e-106
Identity = 195/317 (61.51%), Postives = 244/317 (76.97%), Query Frame = 0

Query: 10  QNERNDVLEAFNPAISNFLAQLSQKVQNSLKPQLNRLANEGQKLTSMSPFAKGERKGSST 69
           +N+ N V   F+PA+S F+   SQK+Q  LK QL  L ++   + S    +  + KG+S+
Sbjct: 28  RNKGNVVSSVFDPALSRFVG-FSQKLQTRLKSQLKNLTDDKHGIGS----SWRQDKGNSS 87

Query: 70  SSLKLDVEKQLQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDAVYNIVTDPD 129
            S+++D+EKQL  WRENPSWTD+ P +KV +PK +L  L A+V+VGLPPDAVYNIV DPD
Sbjct: 88  ISMQIDLEKQLDCWRENPSWTDQIPVVKVGIPKGSLCNLKAEVNVGLPPDAVYNIVIDPD 147

Query: 130 NKRVFKNIKEVISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQNRADHSMKF 189
           N+RVFKNIKEV+SRKVL+D+G RQVVEVEQAALWRFLWWSGTISVHVLVDQ+RADHSMKF
Sbjct: 148 NRRVFKNIKEVMSRKVLVDDGLRQVVEVEQAALWRFLWWSGTISVHVLVDQDRADHSMKF 207

Query: 190 KQLKTGFMKKFEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSRVSLEQLIQP 249
           KQ+K+GFMK+FEG W+V+P+FVDE +C  +KPK L +Y+ CT GKGRIGS+V+L+QLIQP
Sbjct: 208 KQVKSGFMKRFEGSWQVKPLFVDEHMCDRLKPKTLEEYNRCTGGKGRIGSKVTLDQLIQP 267

Query: 250 AIVPPPPISWYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQGTCDSNLLD 309
           AIVPPPPISWYLRGIT +TTEMLI DLLAE  RIR  A G   +        + D   + 
Sbjct: 268 AIVPPPPISWYLRGITAKTTEMLIHDLLAETARIR--AAGVMEDGH------SPDEQRIG 327

Query: 310 SILDIKERWAMHRRNAK 327
           +  DIKERW+ HRR ++
Sbjct: 328 TPGDIKERWSAHRRTSR 331

BLAST of Moc08g00200 vs. TAIR 10
Match: AT1G23560.1 (Domain of unknown function (DUF220) )

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-81
Identity = 152/308 (49.35%), Postives = 206/308 (66.88%), Query Frame = 0

Query: 21  NPAISNFLAQLSQKVQNSLKPQLNRLANEGQ-KLTSMSPFAKGERKGSSTSSLKLDVEKQ 80
           N     FL  ++Q +Q  LK +  RL N  +  L S+S       +      L    EKQ
Sbjct: 24  NVVFPGFLVWMNQTIQEPLKAEFKRLRNVKELSLKSVSKIETAYEEHRDEEKL----EKQ 83

Query: 81  LQAWRENPSWTDKPPQIKVTVPKDTLSRLNAKVDVGLPPDAVYNIVTDPDNKRVFKNIKE 140
           LQAWR+NPSW D+PP++ V         LN + DVGLPP+ VYNI T PDNKR FKNIKE
Sbjct: 84  LQAWRDNPSWIDQPPKVVVKSQNGLFCHLNIEADVGLPPELVYNIFTHPDNKRYFKNIKE 143

Query: 141 VISRKVLIDEGSRQVVEVEQAALWRFLWWSGTISVHVLVDQNRADHSMKFKQLKTGFMKK 200
            ISRKVLIDEG +Q VEV+QAA W+FLWW GT  +H++V++NR + + K+KQ  T FMK 
Sbjct: 144 NISRKVLIDEGPKQTVEVKQAAAWKFLWWDGTCPIHLIVEENRKNLTSKYKQETTMFMKV 203

Query: 201 FEGCWRVEPIFVDESVCFPVKPKNLTDYHACTKGKGRIGSRVSLEQLIQP-AIVPPPPIS 260
           FEGCW+VEP+F+DE +C   KPK+  DYH+C+ G+GRIGS+V+++Q+ QP A++ PPP+S
Sbjct: 204 FEGCWKVEPLFIDEHLCDRSKPKSQKDYHSCSNGRGRIGSKVTMDQMFQPSALLTPPPLS 263

Query: 261 WYLRGITTRTTEMLILDLLAEAKRIREDAKGQTLNNELDISQGTCDSNLLDSILDIKERW 320
           WY+RGIT +TTE +I DL AEA R+R    G  ++++ + +  T + +  D   DIKERW
Sbjct: 264 WYIRGITIKTTESMIEDLFAEATRLRGGKGGGYIDDQGENNVVT-EKSKAD---DIKERW 323

Query: 321 AMHRRNAK 327
             HRR+ +
Sbjct: 324 RSHRRSKR 323

BLAST of Moc08g00200 vs. TAIR 10
Match: AT1G60460.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; Has 23 Blast hits to 23 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 298.1 bits (762), Expect = 2.2e-80
Identity = 176/451 (39.02%), Postives = 252/451 (55.88%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           I+DTG+G  LEEFQ+ +CP E   A+ W                               D
Sbjct: 50  IADTGIGCNLEEFQNLRCPREFNGAKIW-------------------------------D 109

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFR------HGITAVDVL 502
           G++S++TT F D+E+  Y +NL E +  +     PS  K+G KF           ++DVL
Sbjct: 110 GLLSVKTTCFTDDEVYYYHINLDEYIANKRLKRQPSQAKNGAKFSGTEVSLSVFGSMDVL 169

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           +A I  FFQKI +L+I NV ++++V+    PG++   VF  N   +  FTAS L+ LK G
Sbjct: 170 VAPIIGFFQKIIVLQILNVTLDLMVKQGTSPGNQTQYVFAVNADKTPCFTASNLERLKSG 229

Query: 563 LEDYVSRHGSSL--TCDSCFPNRDNLKSGGGMIC-EEKRKTTTLAVEAAIVISELSNPTT 622
           LEDYV RH + L   CD CF +R++LK G G +C E+K K     +E  IVIS+L   T 
Sbjct: 230 LEDYVLRHANCLDTMCDYCFSDREHLKVGSGTVCQEDKHKRVGGTMEVVIVISDLLESTQ 289

Query: 623 NCLGARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHV 682
           +C  +    TEV  F +F P  +    L AL+ IDWK+YGL L +  +Q  H  L+W++ 
Sbjct: 290 HCSRSCNGKTEVLYFDNFLPSPVPHLALSALKKIDWKKYGLILANVNDQDGHVFLEWDNF 349

Query: 683 PLSFHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLK 742
           P    I I +H Y        P  QK      L+ K +K ALD+LK K+ GFLLS+++ K
Sbjct: 350 PSYVQIQIALHWYH----NQYPTRQKNGPGISLLKKGIKNALDNLKAKHEGFLLSSHSRK 409

Query: 743 ISRFAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVND 802
           I  + PDLA++IAGL+  S DLDFQ +CL++LG Q Q+ E + VE  I+ +I++VI +N+
Sbjct: 410 ICSYVPDLARSIAGLIFSSTDLDFQGDCLSVLGFQTQEVERDTVENYIQRKIVTVIGMNE 465

Query: 803 GRPKGTKEVAPLLFGDGRHQLQFVDDECDED 825
            +P+  +E AP LF DG  +  F +DE  ED
Sbjct: 470 RKPQKDQEAAPFLFFDGESETSFFEDEEVED 465

BLAST of Moc08g00200 vs. TAIR 10
Match: AT1G60460.2 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 297.0 bits (759), Expect = 4.8e-80
Identity = 174/447 (38.93%), Postives = 250/447 (55.93%), Query Frame = 0

Query: 383 ISDTGLGSCLEEFQDFKCPVEGILAEQWVAHVQRYFAVGLDYRTIVMRLSINTFLVFYAD 442
           I+DTG+G  LEEFQ+ +CP E   A+ W                               D
Sbjct: 39  IADTGIGCNLEEFQNLRCPREFNGAKIW-------------------------------D 98

Query: 443 GIVSLRTTNFCDNEISCYQLNLKENVTIREPIWLPSNVKHGVKFR------HGITAVDVL 502
           G++S++TT F D+E+  Y +NL E +  +     PS  K+G KF           ++DVL
Sbjct: 99  GLLSVKTTCFTDDEVYYYHINLDEYIANKRLKRQPSQAKNGAKFSGTEVSLSVFGSMDVL 158

Query: 503 LAEINCFFQKIRILKIPNVAMEVVVECQDIPGSRNDAVFLENLSSSSSFTASTLDHLKLG 562
           +A I  FFQKI +L+I NV ++++V+    PG++   VF  N   +  FTAS L+ LK G
Sbjct: 159 VAPIIGFFQKIIVLQILNVTLDLMVKQGTSPGNQTQYVFAVNADKTPCFTASNLERLKSG 218

Query: 563 LEDYVSRHGSSL--TCDSCFPNRDNLKSGGGMIC-EEKRKTTTLAVEAAIVISELSNPTT 622
           LEDYV RH + L   CD CF +R++LK G G +C E+K K     +E  IVIS+L   T 
Sbjct: 219 LEDYVLRHANCLDTMCDYCFSDREHLKVGSGTVCQEDKHKRVGGTMEVVIVISDLLESTQ 278

Query: 623 NCLGARFSDTEVFCFKDFAPCSISEAFLKALRGIDWKRYGLSLESATNQRSHALLKWEHV 682
           +C  +    TEV  F +F P  +    L AL+ IDWK+YGL L +  +Q  H  L+W++ 
Sbjct: 279 HCSRSCNGKTEVLYFDNFLPSPVPHLALSALKKIDWKKYGLILANVNDQDGHVFLEWDNF 338

Query: 683 PLSFHIHIVVHCYQKLVAEVMPLVQKTQFDKKLISKAVKLALDDLKNKYAGFLLSANTLK 742
           P    I I +H Y        P  QK      L+ K +K ALD+LK K+ GFLLS+++ K
Sbjct: 339 PSYVQIQIALHWYH----NQYPTRQKNGPGISLLKKGIKNALDNLKAKHEGFLLSSHSRK 398

Query: 743 ISRFAPDLAKTIAGLVLYSNDLDFQEECLAILGLQPQQSEGEIVEENIKERIISVIEVND 802
           I  + PDLA++IAGL+  S DLDFQ +CL++LG Q Q+ E + VE  I+ +I++VI +N+
Sbjct: 399 ICSYVPDLARSIAGLIFSSTDLDFQGDCLSVLGFQTQEVERDTVENYIQRKIVTVIGMNE 450

Query: 803 GRPKGTKEVAPLLFGDGRHQLQFVDDE 821
            +P+  +E AP LF DG  +  F +DE
Sbjct: 459 RKPQKDQEAAPFLFFDGESETSFFEDE 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131879.11.5e-22990.99type 2 DNA topoisomerase 6 subunit B-like isoform X1 [Momordica charantia][more]
XP_022131880.15.8e-22990.77type 2 DNA topoisomerase 6 subunit B-like isoform X2 [Momordica charantia][more]
KAG7029197.18.2e-19172.31Type 2 DNA topoisomerase 6 subunit B-like protein, partial [Cucurbita argyrosper... [more]
XP_022132255.19.4e-18799.70uncharacterized protein LOC111005155 [Momordica charantia] >XP_022132256.1 uncha... [more]
XP_023545219.11.2e-18472.75type 2 DNA topoisomerase 6 subunit B-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q5Q0E66.8e-7938.93Type 2 DNA topoisomerase 6 subunit B-like OS=Arabidopsis thaliana OX=3702 GN=MTO... [more]
Match NameE-valueIdentityDescription
A0A6J1BRH47.4e-23090.99type 2 DNA topoisomerase 6 subunit B-like isoform X1 OS=Momordica charantia OX=3... [more]
A0A6J1BQX42.8e-22990.77type 2 DNA topoisomerase 6 subunit B-like isoform X2 OS=Momordica charantia OX=3... [more]
A0A6J1BRY34.6e-18799.70uncharacterized protein LOC111005155 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1HCF23.1e-18372.09type 2 DNA topoisomerase 6 subunit B-like isoform X6 OS=Cucurbita moschata OX=36... [more]
A0A6J1K8108.3e-18171.43type 2 DNA topoisomerase 6 subunit B-like isoform X5 OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT1G70480.13.9e-10661.51Domain of unknown function (DUF220) [more]
AT1G70480.23.9e-10661.51Domain of unknown function (DUF220) [more]
AT1G23560.11.5e-8149.35Domain of unknown function (DUF220) [more]
AT1G60460.12.2e-8039.02unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G60460.24.8e-8038.93unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae ... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003863Protein of unknown function DUF220PFAMPF02713DUF220coord: 169..240
e-value: 6.8E-30
score: 103.0
IPR023393START-like domain superfamilyGENE3D3.30.530.20coord: 98..215
e-value: 2.9E-7
score: 32.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..71
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 108..209
IPR034566Type 2 DNA topoisomerase 6 subunit B-like, plantsPANTHERPTHR36722TYPE 2 DNA TOPOISOMERASE 6 SUBUNIT B-LIKEcoord: 364..824

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g00200.1Moc08g00200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042138 meiotic DNA double-strand break formation
molecular_function GO:0016853 isomerase activity