Moc07g26900 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc07g26900
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectinesterase
Locationchr7: 20056677 .. 20058464 (-)
RNA-Seq ExpressionMoc07g26900
SyntenyMoc07g26900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCGGCGATGCTGGTGACCAGAAAAAGAAGAAAATTGCAGTCGTGAGCGTCTCCGCTCTGATCTTGGTGGCGATGGTGGTGGCTGTTGCCGTCGGTGTCAGTAACCCTCACGACGTCGATCACGATGACGGTCACGATACGGAGTCCGACGATGGCCACCAAATTACAACGTCGACAAAATCAATCAAATCCATCTGCCAACCCACTGATTACAAAGACGTTTGCGAAGAAAGCCTTGCGACGGAGGGCGTAACCGCGACGGACCCGAAAGAGTTGATCAAGTCGGCGTTCAAAATAGCCACGAATCACATAAACGCGGCGATTCAAAACTCCACGACTTTGAAAGAGCTGGCCAAAGATCCACGAGCCAACCAAGCCTTACAAAACTGCCGCGAGCTCCTCGAATACGCCATCGACGACATCAAAGAGTCGTTCGAACGAATCGGCACATTTGAAGTGGGCAAAGTCAAAGAGTTCATTGACGATTTAAAAATCTGGCTGACTGGTGCCTTGACCTACGAGGACACTTGTTTGGATGGGTTCGAGAACACCACCGGCGAAGCGGGCGAGAAAATGAGGCAGTTTTTGAAGTCGTCTCAGCAACTTACCAGTAACAGTTTAGCCATGGTTACCGAATTGGCCAACAGTTTGGGCAATCTTCAGATTCCCACATTGGGCCGTCGGCTCATGGCCGAAAACGACGCCCCAGAATGGGTCAGCGACACCAATAGGCGACTGTTGCAGGCTGGGCCGGTGACGGTTAAGCCCAACGTAGTGGTAGCGCAGGACGGAAGTGGGAAATACAAGACCATTAATGAGGCTTTGGTTGAAATTCCCCAAAAGAGTAACGTCACGTTTGTGATCTACGTCAAGGCCGGTATTTATAAGGAAACTGTTGTCATTACGAATGCTATGACCCACGTCACCATGTACGGAGATGGCCCCACCAAAACCGTCGTCACTGGTAGCCTCAACTACATCGACGGTGTTCAAACTTTCAAGACCGCCACCTTCGGTAAGTCCTTTCTTTCTGGATATATTTATATGCTGATCATCGTTAAAAAAATTAATTAATTTAATTTGTGAAATGCAGCGACTATTGGTACCAATTTCTTTGCAAGAGATATGGGTTTCGAGAATAGCGCTGGGCCTGCGAAGCACCAGGCCGTGGCGCTGCGAGTCCAATCCGACAGATCGATCTTCTACAACTGTCGAATGGATGGATACCAAGACACGTTGTACGCACAGACTCACCGTCATTTCTATCGCGACTGCGTCATCACTGGCACCATCGACTTCGTGTTTGGAAACTCAGCCGCCATCTTCCAAAACTGCAAGCTAGTGGTCCGAAAGCCACTGGACAACCAGCAGTGCATCGTGACGGCTCATGGGCGGCTCGGGAAGCGTGAGCCGACTGCACTCGTGTTTCAGGGTTGTCATTTTACATCTGACCCAACGTACTACCCGCACCGAGCCGTGAACAAGGCGTACTTGGGGAGGCCATGGAAGGCATATTCTCGAACCATCATCATGCAGTCTCAAATCGACGACCTAATCCAACCCGAGGGATGGCTACCGTGGATGGGCAACTTTGGGCTGAACACATTGTTCTACGCTGAGTTGCAGAACACAGGACCGGGGGCAGACAAAAGCAAGAGAGTGAAGTGGCGCGGGATTAAAGAGATCACACCGAAACATGCCGTTGATTTCACCCCGAGACCCTTCATCGATGGTGACGCTTGGATCAAGCCTAAAGGAATCCCCTACTCATCTTACATGATGTAA

mRNA sequence

ATGTCCGGCGATGCTGGTGACCAGAAAAAGAAGAAAATTGCAGTCGTGAGCGTCTCCGCTCTGATCTTGGTGGCGATGGTGGTGGCTGTTGCCGTCGGTGTCAGTAACCCTCACGACGTCGATCACGATGACGGTCACGATACGGAGTCCGACGATGGCCACCAAATTACAACGTCGACAAAATCAATCAAATCCATCTGCCAACCCACTGATTACAAAGACGTTTGCGAAGAAAGCCTTGCGACGGAGGGCGTAACCGCGACGGACCCGAAAGAGTTGATCAAGTCGGCGTTCAAAATAGCCACGAATCACATAAACGCGGCGATTCAAAACTCCACGACTTTGAAAGAGCTGGCCAAAGATCCACGAGCCAACCAAGCCTTACAAAACTGCCGCGAGCTCCTCGAATACGCCATCGACGACATCAAAGAGTCGTTCGAACGAATCGGCACATTTGAAGTGGGCAAAGTCAAAGAGTTCATTGACGATTTAAAAATCTGGCTGACTGGTGCCTTGACCTACGAGGACACTTGTTTGGATGGGTTCGAGAACACCACCGGCGAAGCGGGCGAGAAAATGAGGCAGTTTTTGAAGTCGTCTCAGCAACTTACCAGTAACAGTTTAGCCATGGTTACCGAATTGGCCAACAGTTTGGGCAATCTTCAGATTCCCACATTGGGCCGTCGGCTCATGGCCGAAAACGACGCCCCAGAATGGGTCAGCGACACCAATAGGCGACTGTTGCAGGCTGGGCCGGTGACGGTTAAGCCCAACGTAGTGGTAGCGCAGGACGGAAGTGGGAAATACAAGACCATTAATGAGGCTTTGGTTGAAATTCCCCAAAAGAGTAACGTCACGTTTGTGATCTACGTCAAGGCCGGTATTTATAAGGAAACTGTTGTCATTACGAATGCTATGACCCACGTCACCATGTACGGAGATGGCCCCACCAAAACCGTCGTCACTGGTAGCCTCAACTACATCGACGGTGTTCAAACTTTCAAGACCGCCACCTTCGCGACTATTGGTACCAATTTCTTTGCAAGAGATATGGGTTTCGAGAATAGCGCTGGGCCTGCGAAGCACCAGGCCGTGGCGCTGCGAGTCCAATCCGACAGATCGATCTTCTACAACTGTCGAATGGATGGATACCAAGACACGTTGTACGCACAGACTCACCGTCATTTCTATCGCGACTGCGTCATCACTGGCACCATCGACTTCGTGTTTGGAAACTCAGCCGCCATCTTCCAAAACTGCAAGCTAGTGGTCCGAAAGCCACTGGACAACCAGCAGTGCATCGTGACGGCTCATGGGCGGCTCGGGAAGCGTGAGCCGACTGCACTCGTGTTTCAGGGTTGTCATTTTACATCTGACCCAACGTACTACCCGCACCGAGCCGTGAACAAGGCGTACTTGGGGAGGCCATGGAAGGCATATTCTCGAACCATCATCATGCAGTCTCAAATCGACGACCTAATCCAACCCGAGGGATGGCTACCGTGGATGGGCAACTTTGGGCTGAACACATTGTTCTACGCTGAGTTGCAGAACACAGGACCGGGGGCAGACAAAAGCAAGAGAGTGAAGTGGCGCGGGATTAAAGAGATCACACCGAAACATGCCGTTGATTTCACCCCGAGACCCTTCATCGATGGTGACGCTTGGATCAAGCCTAAAGGAATCCCCTACTCATCTTACATGATGTAA

Coding sequence (CDS)

ATGTCCGGCGATGCTGGTGACCAGAAAAAGAAGAAAATTGCAGTCGTGAGCGTCTCCGCTCTGATCTTGGTGGCGATGGTGGTGGCTGTTGCCGTCGGTGTCAGTAACCCTCACGACGTCGATCACGATGACGGTCACGATACGGAGTCCGACGATGGCCACCAAATTACAACGTCGACAAAATCAATCAAATCCATCTGCCAACCCACTGATTACAAAGACGTTTGCGAAGAAAGCCTTGCGACGGAGGGCGTAACCGCGACGGACCCGAAAGAGTTGATCAAGTCGGCGTTCAAAATAGCCACGAATCACATAAACGCGGCGATTCAAAACTCCACGACTTTGAAAGAGCTGGCCAAAGATCCACGAGCCAACCAAGCCTTACAAAACTGCCGCGAGCTCCTCGAATACGCCATCGACGACATCAAAGAGTCGTTCGAACGAATCGGCACATTTGAAGTGGGCAAAGTCAAAGAGTTCATTGACGATTTAAAAATCTGGCTGACTGGTGCCTTGACCTACGAGGACACTTGTTTGGATGGGTTCGAGAACACCACCGGCGAAGCGGGCGAGAAAATGAGGCAGTTTTTGAAGTCGTCTCAGCAACTTACCAGTAACAGTTTAGCCATGGTTACCGAATTGGCCAACAGTTTGGGCAATCTTCAGATTCCCACATTGGGCCGTCGGCTCATGGCCGAAAACGACGCCCCAGAATGGGTCAGCGACACCAATAGGCGACTGTTGCAGGCTGGGCCGGTGACGGTTAAGCCCAACGTAGTGGTAGCGCAGGACGGAAGTGGGAAATACAAGACCATTAATGAGGCTTTGGTTGAAATTCCCCAAAAGAGTAACGTCACGTTTGTGATCTACGTCAAGGCCGGTATTTATAAGGAAACTGTTGTCATTACGAATGCTATGACCCACGTCACCATGTACGGAGATGGCCCCACCAAAACCGTCGTCACTGGTAGCCTCAACTACATCGACGGTGTTCAAACTTTCAAGACCGCCACCTTCGCGACTATTGGTACCAATTTCTTTGCAAGAGATATGGGTTTCGAGAATAGCGCTGGGCCTGCGAAGCACCAGGCCGTGGCGCTGCGAGTCCAATCCGACAGATCGATCTTCTACAACTGTCGAATGGATGGATACCAAGACACGTTGTACGCACAGACTCACCGTCATTTCTATCGCGACTGCGTCATCACTGGCACCATCGACTTCGTGTTTGGAAACTCAGCCGCCATCTTCCAAAACTGCAAGCTAGTGGTCCGAAAGCCACTGGACAACCAGCAGTGCATCGTGACGGCTCATGGGCGGCTCGGGAAGCGTGAGCCGACTGCACTCGTGTTTCAGGGTTGTCATTTTACATCTGACCCAACGTACTACCCGCACCGAGCCGTGAACAAGGCGTACTTGGGGAGGCCATGGAAGGCATATTCTCGAACCATCATCATGCAGTCTCAAATCGACGACCTAATCCAACCCGAGGGATGGCTACCGTGGATGGGCAACTTTGGGCTGAACACATTGTTCTACGCTGAGTTGCAGAACACAGGACCGGGGGCAGACAAAAGCAAGAGAGTGAAGTGGCGCGGGATTAAAGAGATCACACCGAAACATGCCGTTGATTTCACCCCGAGACCCTTCATCGATGGTGACGCTTGGATCAAGCCTAAAGGAATCCCCTACTCATCTTACATGATGTAA

Protein sequence

MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWVSDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVVITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAKHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYSRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKHAVDFTPRPFIDGDAWIKPKGIPYSSYMM
Homology
BLAST of Moc07g26900 vs. NCBI nr
Match: XP_022138440.1 (putative pectinesterase/pectinesterase inhibitor 28 [Momordica charantia])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 569/569 (100.00%), Postives = 569/569 (100.00%), Query Frame = 0

Query: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60
           MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST
Sbjct: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60

Query: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120
           KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK
Sbjct: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120

Query: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180
           DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD
Sbjct: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180

Query: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240
           GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV
Sbjct: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240

Query: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300
           SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV
Sbjct: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300

Query: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360
           VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA
Sbjct: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360

Query: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420
           KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC
Sbjct: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420

Query: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480
           KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY
Sbjct: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480

Query: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540
           SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK
Sbjct: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540

Query: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
           HAVDFTPRPFIDGDAWIKPKGIPYSSYMM
Sbjct: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 569

BLAST of Moc07g26900 vs. NCBI nr
Match: XP_022138455.1 (putative pectinesterase/pectinesterase inhibitor 28 isoform X1 [Momordica charantia])

HSP 1 Score: 964.1 bits (2491), Expect = 5.1e-277
Identity = 474/569 (83.30%), Postives = 517/569 (90.86%), Query Frame = 0

Query: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60
           MSGD G   KK IA+VSV AL++VAMVVAV V  S            + +  G +ITTST
Sbjct: 1   MSGDDGG--KKTIAIVSVCALVVVAMVVAVVVTNSK-----------SAAPSGGEITTST 60

Query: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120
           K+IK+ICQPTDYK++CEESL  EGVT+TDPKEL+KSAFKIAT+++N AIQNSTTLKELAK
Sbjct: 61  KAIKAICQPTDYKEICEESLTKEGVTSTDPKELVKSAFKIATDYVNGAIQNSTTLKELAK 120

Query: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180
           DPRANQALQNCRELLE+AIDDIKESFERIGTF++G+VK F++DLK+WL+GA+TYEDTCLD
Sbjct: 121 DPRANQALQNCRELLEFAIDDIKESFERIGTFQMGEVKTFVEDLKVWLSGAMTYEDTCLD 180

Query: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240
           GFENTTGEAGEKM+QFLKSSQQLTSN LAMVTEL+ +LG+LQ+PTLGRRLM E+D PEWV
Sbjct: 181 GFENTTGEAGEKMKQFLKSSQQLTSNGLAMVTELSKTLGDLQLPTLGRRLMGEDDIPEWV 240

Query: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300
           SDTNRRLLQAGP  VKPNVVVAQDGSGKYKTINEAL EIP+KSN+TFVI+VKAGIYKETV
Sbjct: 241 SDTNRRLLQAGPPAVKPNVVVAQDGSGKYKTINEALAEIPKKSNITFVIHVKAGIYKETV 300

Query: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360
           VIT AMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFA IG NFFARDMGFENSAGP 
Sbjct: 301 VITKAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFAAIGANFFARDMGFENSAGPT 360

Query: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420
           KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHR FYRDCVITGTIDFVFGNSAA+FQNC
Sbjct: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRQFYRDCVITGTIDFVFGNSAAVFQNC 420

Query: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480
           +LVVRKP+DNQQCIVTA GRL +REPTALVFQ CHFTSDP YYPHRAVNKAYLGRPWKAY
Sbjct: 421 QLVVRKPMDNQQCIVTAQGRLEEREPTALVFQSCHFTSDPAYYPHRAVNKAYLGRPWKAY 480

Query: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540
           SRTIIMQSQIDDLIQPEGWLPWMG FGL+TLFYAELQNTGPG+DKSKRVKW GIKEITP+
Sbjct: 481 SRTIIMQSQIDDLIQPEGWLPWMGTFGLDTLFYAELQNTGPGSDKSKRVKWAGIKEITPE 540

Query: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
            A+ FTP+PFIDGDAWIK K +PYSS MM
Sbjct: 541 QALAFTPKPFIDGDAWIKDKRLPYSSNMM 556

BLAST of Moc07g26900 vs. NCBI nr
Match: XP_022138456.1 (putative pectinesterase/pectinesterase inhibitor 28 isoform X2 [Momordica charantia])

HSP 1 Score: 941.0 bits (2431), Expect = 4.7e-270
Identity = 461/569 (81.02%), Postives = 509/569 (89.46%), Query Frame = 0

Query: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60
           MSGD G   KK IA+VSV AL++VAMVVAV V  S            + +  G +ITTST
Sbjct: 1   MSGDDGG--KKTIAIVSVCALVVVAMVVAVVVTNSK-----------SAAPSGGEITTST 60

Query: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120
           K+IK+ICQPTDYK++CEESL  EGVT+TDPKEL+KSAFKIAT+++N AIQNSTTLKELAK
Sbjct: 61  KAIKAICQPTDYKEICEESLTKEGVTSTDPKELVKSAFKIATDYVNGAIQNSTTLKELAK 120

Query: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180
           DPRANQALQNCRELLE+AIDDIKESFERIGTF++G+VK F++DLK+WL+GA+TYEDTCLD
Sbjct: 121 DPRANQALQNCRELLEFAIDDIKESFERIGTFQMGEVKTFVEDLKVWLSGAMTYEDTCLD 180

Query: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240
           GFENTTGEAGEKM+QFLKSSQQLTSN LAMVTEL+ +LG+LQ+PTLGRRLM E+   EWV
Sbjct: 181 GFENTTGEAGEKMKQFLKSSQQLTSNGLAMVTELSKTLGDLQLPTLGRRLMGEDGISEWV 240

Query: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300
           SDTNRRLLQAGP  VKPNVVVAQDGSGKYKTINEAL EIP+ SNVTFVI+VKAGIYKE V
Sbjct: 241 SDTNRRLLQAGPPAVKPNVVVAQDGSGKYKTINEALAEIPKNSNVTFVIHVKAGIYKEHV 300

Query: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360
           VIT AMTHVTMYGDGPTKTVVTGSLNY+DG+QTFKTA+FA IG NFFARDMGFEN+AGP 
Sbjct: 301 VITKAMTHVTMYGDGPTKTVVTGSLNYVDGIQTFKTASFAAIGANFFARDMGFENTAGPT 360

Query: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420
           KHQAVALRVQSDRSIFYNCRMDGYQDTLY Q HR FYRDCVITGTIDFVFGN+AA+FQNC
Sbjct: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYTQAHRQFYRDCVITGTIDFVFGNAAAVFQNC 420

Query: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480
           +LVVRKP+DNQQCIVTA GRL +REPTALVFQ CHFTSDP YYPHRAVNKAYLGRPWKAY
Sbjct: 421 QLVVRKPMDNQQCIVTAQGRLEEREPTALVFQSCHFTSDPAYYPHRAVNKAYLGRPWKAY 480

Query: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540
           SRTIIMQS+IDDLIQPEGWLPWMG FGL+TLFY E QNTGPGA+KSKRV+W GIKEITP+
Sbjct: 481 SRTIIMQSKIDDLIQPEGWLPWMGTFGLDTLFYVEFQNTGPGANKSKRVEWAGIKEITPQ 540

Query: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
             + FTP+PFIDGDAWIK KGIPYSS MM
Sbjct: 541 QILAFTPKPFIDGDAWIKDKGIPYSSNMM 556

BLAST of Moc07g26900 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 866.3 bits (2237), Expect = 1.5e-247
Identity = 415/567 (73.19%), Postives = 488/567 (86.07%), Query Frame = 0

Query: 6   GDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSIKS 65
           GD+KKKK+A++SVS+LILVAMVVAV VGV+N    +H DGH  E+   H+I+++TK+I++
Sbjct: 10  GDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPKEEHGDGHGGET---HEISSTTKAIQA 69

Query: 66  ICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPRAN 125
           ICQPTDYKDVCE++L  E    TDPKEL K   +IAT ++N AI+NSTTL+ELAKDPR N
Sbjct: 70  ICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQELAKDPRTN 129

Query: 126 QALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFENT 185
           QALQNCRELLEYAIDD+ ESFERIGTF++ K+ EF+ DLKIWL+GALTYE TCLDGFENT
Sbjct: 130 QALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENT 189

Query: 186 TGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDA---PEWVSD 245
           TG+AG +M++FLKSSQQ+T+N LA+V EL+  LG+LQIP +  R + E+DA   P WVS+
Sbjct: 190 TGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQIPGMTGRRLLEDDADEMPSWVSE 249

Query: 246 TNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVVI 305
             RRL+QA   ++KP++VVAQDGSGKYKTIN AL ++P KSN TFVIYVKAGIYKE VVI
Sbjct: 250 GKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGIYKEIVVI 309

Query: 306 TNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAKH 365
             AMTH+TMYGDGPTKT+VTGSLN+IDG+QTFKTATFA IG NF+ARDMGFEN+AG AKH
Sbjct: 310 PKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDMGFENTAGAAKH 369

Query: 366 QAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCKL 425
           QAVALRVQSDRSIFYNC++DGYQDTLYA  HR FYRDC+I+GTIDFVFGN+A IFQNCKL
Sbjct: 370 QAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFGNAATIFQNCKL 429

Query: 426 VVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYSR 485
           VVRKP+DNQQCIVTAHGRL ++EPTAL+FQ CHF  DP Y P + +NKAYLGRPWK YSR
Sbjct: 430 VVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKAYLGRPWKQYSR 489

Query: 486 TIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKHA 545
           TII+ SQIDD+IQPEGWLPWMG+FGLNTLFYAE+QN GPGAD+SKRVKWRGI+ ITP+HA
Sbjct: 490 TIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKWRGIRHITPQHA 549

Query: 546 VDFTPRPFIDGDAWIKPKGIPYSSYMM 570
            D+TPR FIDGDAWI  KGIPYSS MM
Sbjct: 550 ADYTPRRFIDGDAWIPAKGIPYSSGMM 573

BLAST of Moc07g26900 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 856.3 bits (2211), Expect = 1.5e-244
Identity = 417/569 (73.29%), Postives = 487/569 (85.59%), Query Frame = 0

Query: 6   GDQKKKKIAVVSVSALILVAMVVAVAVGVSN-PHDVDH-DDGHDTESDDGHQITTSTKSI 65
           GD+KKKKIA++SVS+LILVAMVVAV VGV+N PH  +H DD H +E+    +IT+STK+I
Sbjct: 10  GDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTP--EITSSTKAI 69

Query: 66  KSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPR 125
           ++ICQPTDYK+ CE+SL  E    TDPKEL+K  FKIAT  +N AI+NSTTLKELAKDPR
Sbjct: 70  QAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKELAKDPR 129

Query: 126 ANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFE 185
            NQALQNCRELLEYAIDD+ +SFERIGTF++ K+ +F+ DLKIWL+GALTYE TCLDGFE
Sbjct: 130 TNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGFE 189

Query: 186 NTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTL-GRRLMAEND--APEWV 245
           NTTG+AG +M++FLKS+QQ+T+N L +V EL+  LG+LQ+P + GRRL+ ++D   P WV
Sbjct: 190 NTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDDREMPSWV 249

Query: 246 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 305
           SD  RRL+QAG   +KP++VVAQDGSGKYKTIN AL ++P KSN TFVI+VKAGIYKE V
Sbjct: 250 SDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKAGIYKEIV 309

Query: 306 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 365
           VI   MTH+TMYGDGPTKTVVTGSLN+IDG+QTFKTATF+ IG NF+ARDMGFEN+AG A
Sbjct: 310 VIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGAA 369

Query: 366 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 425
           KHQAVALRVQSDRSIF+NC++DGYQDTLYA  HR FYRDC I+GTIDFVFGN+A  FQNC
Sbjct: 370 KHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNC 429

Query: 426 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 485
           KLVVRKPLDNQQCIVTAHGRL ++EPTAL+FQ CHF  DP Y P +A+NKAYLGRPWK Y
Sbjct: 430 KLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEY 489

Query: 486 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 545
           SRTII+ S IDD+IQPEGWLPWMG+FGLNTLFYAE+QN G GAD+SKRVKWRGIK ITP+
Sbjct: 490 SRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQ 549

Query: 546 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
           HA DFTPR FIDGDAWI  KGIPYSS MM
Sbjct: 550 HAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of Moc07g26900 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 3.3e-178
Identity = 298/561 (53.12%), Postives = 399/561 (71.12%), Query Frame = 0

Query: 4   DAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSI 63
           D   ++KK+  ++S+S+++L++MVVAV +GVS    V+  D    E     +ITTS K+I
Sbjct: 7   DEDAKRKKRYVIISISSVLLISMVVAVTIGVS----VNKSDNAGDE-----EITTSVKAI 66

Query: 64  KSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPR 123
           K +C PTDYK+ CE++L  +    +DP EL+K+AF      I+   + S T+ EL KDPR
Sbjct: 67  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 126

Query: 124 ANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFE 183
           A  AL  C+EL++YAI ++ +SFE +G FE  KV E +  L+IWL+  +++E TCLDGF+
Sbjct: 127 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 186

Query: 184 NTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWVSDT 243
            T G AGE +++ LK++ QLT N LAMVTE++N LG +QIP +  R +   + P W+   
Sbjct: 187 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDAR 246

Query: 244 NRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVVIT 303
            RRLL A    VKP++VVAQDGSG+YKTINEAL  +P+K N TFV+++K GIYKE V + 
Sbjct: 247 ARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVN 306

Query: 304 NAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAKHQ 363
            +MTH+   GDGP KTV++GS +Y DG+ T+KTAT A +G +F A+++ FEN+AG  KHQ
Sbjct: 307 RSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQ 366

Query: 364 AVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCKLV 423
           AVA+RV +D SIFYNC+ DGYQDTLYA +HR FYRDC I+GTIDF+FG++AA+FQNC L+
Sbjct: 367 AVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLL 426

Query: 424 VRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYSRT 483
           VRKPL NQ C +TAHGR   RE T  V QGC    +P Y   +  +K YLGRPWK YSRT
Sbjct: 427 VRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRT 486

Query: 484 IIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKHAV 543
           IIM + I D + PEGW PW+G FGLNTLFY+E+QNTGPGA  +KRV W GIK+++ +  +
Sbjct: 487 IIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEIL 546

Query: 544 DFTPRPFIDGDAWIKPKGIPY 565
            FTP  +I GDAWI  KG+PY
Sbjct: 547 KFTPAQYIQGDAWIPGKGVPY 558

BLAST of Moc07g26900 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 618.2 bits (1593), Expect = 9.1e-176
Identity = 286/568 (50.35%), Postives = 402/568 (70.77%), Query Frame = 0

Query: 4   DAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSI 63
           D   ++K++  V+++S+++L++MVVAV VGVS        + HD +S    ++  S K++
Sbjct: 6   DDESKRKRRYIVITISSVLLISMVVAVTVGVSL-------NKHDGDSKGKAEVNASVKAV 65

Query: 64  KSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPR 123
           K +C PTDY+  CE++L   G   TDP EL+K+AF +    I  A + S T+ EL KD R
Sbjct: 66  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 125

Query: 124 ANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFE 183
              AL  C+EL++YA+D++  SFE +G FE   + E + +L+IWL+ A+++E+TCL+GF+
Sbjct: 126 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 185

Query: 184 NTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWVSDT 243
            T G AGE M++ LK++ +LT N LA+++E++N +G +QIP L  R +     P WV   
Sbjct: 186 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQR 245

Query: 244 NRRLLQAGPV--TVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVV 303
            R+LLQA      VKP++VVAQDGSG+YKTINEAL  +P+K N TFV+++KAG+YKE V 
Sbjct: 246 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 305

Query: 304 ITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAK 363
           +   M+H+   GDGP KT+++G+ NY DG+ T++TAT A +G  F A+++GFEN+AG  K
Sbjct: 306 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 365

Query: 364 HQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCK 423
           HQAVA+RVQSD SIF+NCR DGYQDTLY  +HR F+RDC I+GTIDF+FG++AA+FQNC 
Sbjct: 366 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 425

Query: 424 LVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYS 483
           L+VRKPL NQ C +TAHGR   RE T  VFQGC    +P Y   +  +KAYLGRPWK YS
Sbjct: 426 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 485

Query: 484 RTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKH 543
           RTIIM + I D +QP+GW PW+G+FGL TLFY+E+QNTGPG+  + RV W GIK ++ + 
Sbjct: 486 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 545

Query: 544 AVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
            + FTP  +I GD WI  KG+PY++ ++
Sbjct: 546 ILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Moc07g26900 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 5.0e-158
Identity = 273/573 (47.64%), Postives = 383/573 (66.84%), Query Frame = 0

Query: 3   GDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKS 62
           G  G+ KKKK  +  V   +LV MV  VAVG++   +  H     +E     QI T+T +
Sbjct: 2   GVDGELKKKKCIIAGVITALLVLMV--VAVGITTSRNTSH-----SEKIVPVQIKTATTA 61

Query: 63  IKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTT--LKELAK 122
           ++++C PTDYK+ C  SL      +T P +LIK  F +    I  +I+ ++     + A 
Sbjct: 62  VEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAN 121

Query: 123 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 182
           D     AL+ C +L+  A DD+K+  +    F + ++++F++DL++WL+G++ Y+ TC+D
Sbjct: 122 DKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMD 181

Query: 183 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIP----TLG----RRLMA 242
            FE T  +  + M++  K+S++LTSN LAM+T ++N LG   +      LG    + L A
Sbjct: 182 TFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA 241

Query: 243 ENDAPEWVSDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVK 302
           E+  P WV    RRL+ A    VK NVVVA DGSG+YKTINEAL  +P+ +   FVIY+K
Sbjct: 242 EDGIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIK 301

Query: 303 AGIYKETVVITNAMTHVTMYGDGPTKTVVTGSLNYIDG-VQTFKTATFATIGTNFFARDM 362
            G+Y E V +T  MTHVT  GDGPTKT +TGSLNY  G V+T+ TAT A  G NF A+++
Sbjct: 302 QGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNI 361

Query: 363 GFENSAGPAKHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFG 422
           GFEN+AGP  HQAVALRV +D ++FYNC++DGYQDTLY  +HR F+RDC ++GT+DF+FG
Sbjct: 362 GFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFG 421

Query: 423 NSAAIFQNCKLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKA 482
           +   + QNC +VVRKP+ +Q C++TA GR  KRE T LV Q CH T +P Y P +++NKA
Sbjct: 422 DGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKA 481

Query: 483 YLGRPWKAYSRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKW 542
           YLGRPWK +SRTIIM + IDD+I P GWLPW G+F LNTL+YAE +N GPG+++++RVKW
Sbjct: 482 YLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKW 541

Query: 543 RGIKEITPKHAVDFTPRPFIDGDAWIKPKGIPY 565
            GIK+++PK A+ FTP  F+ G+ WI P  +PY
Sbjct: 542 PGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Moc07g26900 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 271/578 (46.89%), Postives = 383/578 (66.26%), Query Frame = 0

Query: 3   GDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKS 62
           G  GD+KKK I   SVS   LV MVV+VAV  S          H  + D+ H I  +TK+
Sbjct: 2   GSDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSK---------HSPKDDENH-IRKTTKA 61

Query: 63  IKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQN-STTLKELA-K 122
           ++++C PTD+KD C  SL      + DP +LIK  FK+    IN +++  S  +K  A K
Sbjct: 62  VQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADK 121

Query: 123 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 182
           +P A  A + C +L+  AIDD+K+  +    F V +++ F++DL++WL+G++ ++ TC+D
Sbjct: 122 NPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMD 181

Query: 183 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTL-------GRRLMAE 242
            F        + M +  K+S++L+SNSLAMVT ++  + N  +  L        R+L++ 
Sbjct: 182 SFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST 241

Query: 243 NDA-PEWVSDTNRRLLQA---GPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVI 302
            D+ P WV    RRL+ A   GP  VK N VVAQDG+G++KTI +AL  +P+ + V F+I
Sbjct: 242 EDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFII 301

Query: 303 YVKAGIYKETVVITNAMTHVTMYGDGPTKTVVTGSLNY-IDGVQTFKTATFATIGTNFFA 362
           ++K GIYKE V +T  M HVT  GDGP KT++TGSLN+ I  V+TF TAT    G +F A
Sbjct: 302 HIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTA 361

Query: 363 RDMGFENSAGPAKHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDF 422
           +++G EN+AGP   QAVALRV +D ++F++C++DG+QDTLY  +HR FYRDC ++GT+DF
Sbjct: 362 KNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDF 421

Query: 423 VFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAV 482
           +FG++  I QNCK+VVRKP   Q C+VTA GR   RE T LV  GCH T DP Y P ++V
Sbjct: 422 IFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSV 481

Query: 483 NKAYLGRPWKAYSRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKR 542
           NKAYLGRPWK +SRTIIM++ IDD+I P GWLPW G+F L TL+YAE  NTGPG+++++R
Sbjct: 482 NKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQR 541

Query: 543 VKWRGIKEITPKHAVDFTPRPFIDGDAWIKPKGIPYSS 567
           VKW GIK++TP+ A+ +T   F+ GD WI    +PY++
Sbjct: 542 VKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Moc07g26900 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 3.1e-131
Identity = 228/446 (51.12%), Postives = 303/446 (67.94%), Query Frame = 0

Query: 128 LQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFENTTG 187
           ++ C E+L+YA+D I +S   +  F+  K+ E+  DLK+WLTG L+++ TCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 188 EAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIP--TLGRRLMAENDAPEWVSDTNR 247
           +AGE M + LK+S +L+SN++ M+  ++  L        ++ RRL++++  P WV+D +R
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGIPSWVNDGHR 120

Query: 248 RLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVVITNA 307
           RLL  G   V+PN VVAQDGSG++KT+ +AL  +P K+ V FVI+VKAG+YKETV +   
Sbjct: 121 RLLAGG--NVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVAKE 180

Query: 308 MTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAKHQAV 367
           M +VT+ GDGPTKT  TGSLNY DG+ T+ TATF   G NF A+D+GFEN+AG  KHQAV
Sbjct: 181 MNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAV 240

Query: 368 ALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCKLVVR 427
           ALRV +D++IFYNC+MDG+QDTLY Q+ R FYRDC I+GTIDFVFG    +FQNCKLV R
Sbjct: 241 ALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCR 300

Query: 428 KPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYSRTII 487
            P   QQC+VTA GR  +   +ALVFQ  HFT +P          +YLGRPWK YS+ +I
Sbjct: 301 LPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKVVI 360

Query: 488 MQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKHAVDF 547
           M S ID +  PEG++PWMG+    T  Y E  N GPGAD + RVKW G+K +T   A ++
Sbjct: 361 MDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAEY 420

Query: 548 TPRPFID------GDAWIKPKGIPYS 566
            P  F +       D WI   G+PYS
Sbjct: 421 YPGKFFEIVNATARDTWIVKSGVPYS 443

BLAST of Moc07g26900 vs. ExPASy TrEMBL
Match: A0A6J1C9G4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009613 PE=3 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 569/569 (100.00%), Postives = 569/569 (100.00%), Query Frame = 0

Query: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60
           MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST
Sbjct: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60

Query: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120
           KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK
Sbjct: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120

Query: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180
           DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD
Sbjct: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180

Query: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240
           GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV
Sbjct: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240

Query: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300
           SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV
Sbjct: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300

Query: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360
           VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA
Sbjct: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360

Query: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420
           KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC
Sbjct: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420

Query: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480
           KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY
Sbjct: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480

Query: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540
           SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK
Sbjct: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540

Query: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
           HAVDFTPRPFIDGDAWIKPKGIPYSSYMM
Sbjct: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 569

BLAST of Moc07g26900 vs. ExPASy TrEMBL
Match: A0A6J1C9H6 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009624 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.5e-277
Identity = 474/569 (83.30%), Postives = 517/569 (90.86%), Query Frame = 0

Query: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60
           MSGD G   KK IA+VSV AL++VAMVVAV V  S            + +  G +ITTST
Sbjct: 1   MSGDDGG--KKTIAIVSVCALVVVAMVVAVVVTNSK-----------SAAPSGGEITTST 60

Query: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120
           K+IK+ICQPTDYK++CEESL  EGVT+TDPKEL+KSAFKIAT+++N AIQNSTTLKELAK
Sbjct: 61  KAIKAICQPTDYKEICEESLTKEGVTSTDPKELVKSAFKIATDYVNGAIQNSTTLKELAK 120

Query: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180
           DPRANQALQNCRELLE+AIDDIKESFERIGTF++G+VK F++DLK+WL+GA+TYEDTCLD
Sbjct: 121 DPRANQALQNCRELLEFAIDDIKESFERIGTFQMGEVKTFVEDLKVWLSGAMTYEDTCLD 180

Query: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240
           GFENTTGEAGEKM+QFLKSSQQLTSN LAMVTEL+ +LG+LQ+PTLGRRLM E+D PEWV
Sbjct: 181 GFENTTGEAGEKMKQFLKSSQQLTSNGLAMVTELSKTLGDLQLPTLGRRLMGEDDIPEWV 240

Query: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300
           SDTNRRLLQAGP  VKPNVVVAQDGSGKYKTINEAL EIP+KSN+TFVI+VKAGIYKETV
Sbjct: 241 SDTNRRLLQAGPPAVKPNVVVAQDGSGKYKTINEALAEIPKKSNITFVIHVKAGIYKETV 300

Query: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360
           VIT AMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFA IG NFFARDMGFENSAGP 
Sbjct: 301 VITKAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFAAIGANFFARDMGFENSAGPT 360

Query: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420
           KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHR FYRDCVITGTIDFVFGNSAA+FQNC
Sbjct: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRQFYRDCVITGTIDFVFGNSAAVFQNC 420

Query: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480
           +LVVRKP+DNQQCIVTA GRL +REPTALVFQ CHFTSDP YYPHRAVNKAYLGRPWKAY
Sbjct: 421 QLVVRKPMDNQQCIVTAQGRLEEREPTALVFQSCHFTSDPAYYPHRAVNKAYLGRPWKAY 480

Query: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540
           SRTIIMQSQIDDLIQPEGWLPWMG FGL+TLFYAELQNTGPG+DKSKRVKW GIKEITP+
Sbjct: 481 SRTIIMQSQIDDLIQPEGWLPWMGTFGLDTLFYAELQNTGPGSDKSKRVKWAGIKEITPE 540

Query: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
            A+ FTP+PFIDGDAWIK K +PYSS MM
Sbjct: 541 QALAFTPKPFIDGDAWIKDKRLPYSSNMM 556

BLAST of Moc07g26900 vs. ExPASy TrEMBL
Match: A0A6J1C9S1 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009624 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.3e-270
Identity = 461/569 (81.02%), Postives = 509/569 (89.46%), Query Frame = 0

Query: 1   MSGDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTST 60
           MSGD G   KK IA+VSV AL++VAMVVAV V  S            + +  G +ITTST
Sbjct: 1   MSGDDGG--KKTIAIVSVCALVVVAMVVAVVVTNSK-----------SAAPSGGEITTST 60

Query: 61  KSIKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAK 120
           K+IK+ICQPTDYK++CEESL  EGVT+TDPKEL+KSAFKIAT+++N AIQNSTTLKELAK
Sbjct: 61  KAIKAICQPTDYKEICEESLTKEGVTSTDPKELVKSAFKIATDYVNGAIQNSTTLKELAK 120

Query: 121 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 180
           DPRANQALQNCRELLE+AIDDIKESFERIGTF++G+VK F++DLK+WL+GA+TYEDTCLD
Sbjct: 121 DPRANQALQNCRELLEFAIDDIKESFERIGTFQMGEVKTFVEDLKVWLSGAMTYEDTCLD 180

Query: 181 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWV 240
           GFENTTGEAGEKM+QFLKSSQQLTSN LAMVTEL+ +LG+LQ+PTLGRRLM E+   EWV
Sbjct: 181 GFENTTGEAGEKMKQFLKSSQQLTSNGLAMVTELSKTLGDLQLPTLGRRLMGEDGISEWV 240

Query: 241 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 300
           SDTNRRLLQAGP  VKPNVVVAQDGSGKYKTINEAL EIP+ SNVTFVI+VKAGIYKE V
Sbjct: 241 SDTNRRLLQAGPPAVKPNVVVAQDGSGKYKTINEALAEIPKNSNVTFVIHVKAGIYKEHV 300

Query: 301 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 360
           VIT AMTHVTMYGDGPTKTVVTGSLNY+DG+QTFKTA+FA IG NFFARDMGFEN+AGP 
Sbjct: 301 VITKAMTHVTMYGDGPTKTVVTGSLNYVDGIQTFKTASFAAIGANFFARDMGFENTAGPT 360

Query: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 420
           KHQAVALRVQSDRSIFYNCRMDGYQDTLY Q HR FYRDCVITGTIDFVFGN+AA+FQNC
Sbjct: 361 KHQAVALRVQSDRSIFYNCRMDGYQDTLYTQAHRQFYRDCVITGTIDFVFGNAAAVFQNC 420

Query: 421 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 480
           +LVVRKP+DNQQCIVTA GRL +REPTALVFQ CHFTSDP YYPHRAVNKAYLGRPWKAY
Sbjct: 421 QLVVRKPMDNQQCIVTAQGRLEEREPTALVFQSCHFTSDPAYYPHRAVNKAYLGRPWKAY 480

Query: 481 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 540
           SRTIIMQS+IDDLIQPEGWLPWMG FGL+TLFY E QNTGPGA+KSKRV+W GIKEITP+
Sbjct: 481 SRTIIMQSKIDDLIQPEGWLPWMGTFGLDTLFYVEFQNTGPGANKSKRVEWAGIKEITPQ 540

Query: 541 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
             + FTP+PFIDGDAWIK KGIPYSS MM
Sbjct: 541 QILAFTPKPFIDGDAWIKDKGIPYSSNMM 556

BLAST of Moc07g26900 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 7.3e-245
Identity = 417/569 (73.29%), Postives = 487/569 (85.59%), Query Frame = 0

Query: 6   GDQKKKKIAVVSVSALILVAMVVAVAVGVSN-PHDVDH-DDGHDTESDDGHQITTSTKSI 65
           GD+KKKKIA++SVS+LILVAMVVAV VGV+N PH  +H DD H +E+    +IT+STK+I
Sbjct: 10  GDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTP--EITSSTKAI 69

Query: 66  KSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPR 125
           ++ICQPTDYK+ CE+SL  E    TDPKEL+K  FKIAT  +N AI+NSTTLKELAKDPR
Sbjct: 70  QAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKELAKDPR 129

Query: 126 ANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFE 185
            NQALQNCRELLEYAIDD+ +SFERIGTF++ K+ +F+ DLKIWL+GALTYE TCLDGFE
Sbjct: 130 TNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTCLDGFE 189

Query: 186 NTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTL-GRRLMAEND--APEWV 245
           NTTG+AG +M++FLKS+QQ+T+N L +V EL+  LG+LQ+P + GRRL+ ++D   P WV
Sbjct: 190 NTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDDREMPSWV 249

Query: 246 SDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETV 305
           SD  RRL+QAG   +KP++VVAQDGSGKYKTIN AL ++P KSN TFVI+VKAGIYKE V
Sbjct: 250 SDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKAGIYKEIV 309

Query: 306 VITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPA 365
           VI   MTH+TMYGDGPTKTVVTGSLN+IDG+QTFKTATF+ IG NF+ARDMGFEN+AG A
Sbjct: 310 VIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGAA 369

Query: 366 KHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNC 425
           KHQAVALRVQSDRSIF+NC++DGYQDTLYA  HR FYRDC I+GTIDFVFGN+A  FQNC
Sbjct: 370 KHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNC 429

Query: 426 KLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAY 485
           KLVVRKPLDNQQCIVTAHGRL ++EPTAL+FQ CHF  DP Y P +A+NKAYLGRPWK Y
Sbjct: 430 KLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYLGRPWKEY 489

Query: 486 SRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPK 545
           SRTII+ S IDD+IQPEGWLPWMG+FGLNTLFYAE+QN G GAD+SKRVKWRGIK ITP+
Sbjct: 490 SRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRGIKHITPQ 549

Query: 546 HAVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
           HA DFTPR FIDGDAWI  KGIPYSS MM
Sbjct: 550 HAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of Moc07g26900 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.4e-243
Identity = 410/567 (72.31%), Postives = 485/567 (85.54%), Query Frame = 0

Query: 6   GDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSIKS 65
           GD+KKKKIA++SVS+LILVAMVVAV VG++N    +H +G D  S    +I++STK+I++
Sbjct: 10  GDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEG-DQGSKTTPEISSSTKAIQA 69

Query: 66  ICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPRAN 125
           ICQPTDYK+ CE+SL  E    TDPKEL+K  FKIAT  +N AI+NSTTL+EL+KDPR N
Sbjct: 70  ICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELSKDPRTN 129

Query: 126 QALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFENT 185
           QALQNCRELLEYAIDD+ ESFERIGTF++ K+ EF+ DLKIWL+GALTYE TCLDGFENT
Sbjct: 130 QALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENT 189

Query: 186 TGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTL-GRRLMAEND--APEWVSD 245
           TG+AG +M++FLKS+Q++T+N L +V EL+  LG+LQIP + GRRL+ ++D   P W+SD
Sbjct: 190 TGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDHEMPSWISD 249

Query: 246 TNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVVI 305
             RRL+QAG   +KP+++VAQDGSGKYKTIN AL ++P KSN TFVIYVKAGIYKE VVI
Sbjct: 250 GKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAGIYKEIVVI 309

Query: 306 TNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAKH 365
              MTH+TMYGDGPTKTVVTGSLN+IDG+QTFKTATF+ IG NF+ARDMGFEN+AG AKH
Sbjct: 310 PKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGSAKH 369

Query: 366 QAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCKL 425
           QAVALRVQSDRSIFYNC++DGYQDTLYA  HR FYRDC I+GTIDFVFGN+A  FQNCKL
Sbjct: 370 QAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKL 429

Query: 426 VVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYSR 485
           VVRKP+DNQQCIVTAHGR+ ++EPTAL+FQGCHF  DP Y P +++NKAYLGRPWK YSR
Sbjct: 430 VVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLGRPWKQYSR 489

Query: 486 TIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKHA 545
           TII+ SQIDD+IQPEGWLPWMG+FGLNTLFYAE+QN GPGA ++KRVKWRGIK ITP+HA
Sbjct: 490 TIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGIKHITPQHA 549

Query: 546 VDFTPRPFIDGDAWIKPKGIPYSSYMM 570
            DFTPR FIDGDAWI  KGIPYSS MM
Sbjct: 550 ADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of Moc07g26900 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 626.3 bits (1614), Expect = 2.4e-179
Identity = 298/561 (53.12%), Postives = 399/561 (71.12%), Query Frame = 0

Query: 4   DAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSI 63
           D   ++KK+  ++S+S+++L++MVVAV +GVS    V+  D    E     +ITTS K+I
Sbjct: 7   DEDAKRKKRYVIISISSVLLISMVVAVTIGVS----VNKSDNAGDE-----EITTSVKAI 66

Query: 64  KSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPR 123
           K +C PTDYK+ CE++L  +    +DP EL+K+AF      I+   + S T+ EL KDPR
Sbjct: 67  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 126

Query: 124 ANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFE 183
           A  AL  C+EL++YAI ++ +SFE +G FE  KV E +  L+IWL+  +++E TCLDGF+
Sbjct: 127 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 186

Query: 184 NTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWVSDT 243
            T G AGE +++ LK++ QLT N LAMVTE++N LG +QIP +  R +   + P W+   
Sbjct: 187 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDAR 246

Query: 244 NRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVVIT 303
            RRLL A    VKP++VVAQDGSG+YKTINEAL  +P+K N TFV+++K GIYKE V + 
Sbjct: 247 ARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVN 306

Query: 304 NAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAKHQ 363
            +MTH+   GDGP KTV++GS +Y DG+ T+KTAT A +G +F A+++ FEN+AG  KHQ
Sbjct: 307 RSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQ 366

Query: 364 AVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCKLV 423
           AVA+RV +D SIFYNC+ DGYQDTLYA +HR FYRDC I+GTIDF+FG++AA+FQNC L+
Sbjct: 367 AVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLL 426

Query: 424 VRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYSRT 483
           VRKPL NQ C +TAHGR   RE T  V QGC    +P Y   +  +K YLGRPWK YSRT
Sbjct: 427 VRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRT 486

Query: 484 IIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKHAV 543
           IIM + I D + PEGW PW+G FGLNTLFY+E+QNTGPGA  +KRV W GIK+++ +  +
Sbjct: 487 IIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEIL 546

Query: 544 DFTPRPFIDGDAWIKPKGIPY 565
            FTP  +I GDAWI  KG+PY
Sbjct: 547 KFTPAQYIQGDAWIPGKGVPY 558

BLAST of Moc07g26900 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 618.2 bits (1593), Expect = 6.5e-177
Identity = 286/568 (50.35%), Postives = 402/568 (70.77%), Query Frame = 0

Query: 4   DAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSI 63
           D   ++K++  V+++S+++L++MVVAV VGVS        + HD +S    ++  S K++
Sbjct: 6   DDESKRKRRYIVITISSVLLISMVVAVTVGVSL-------NKHDGDSKGKAEVNASVKAV 65

Query: 64  KSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTTLKELAKDPR 123
           K +C PTDY+  CE++L   G   TDP EL+K+AF +    I  A + S T+ EL KD R
Sbjct: 66  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 125

Query: 124 ANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFE 183
              AL  C+EL++YA+D++  SFE +G FE   + E + +L+IWL+ A+++E+TCL+GF+
Sbjct: 126 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 185

Query: 184 NTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTLGRRLMAENDAPEWVSDT 243
            T G AGE M++ LK++ +LT N LA+++E++N +G +QIP L  R +     P WV   
Sbjct: 186 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQR 245

Query: 244 NRRLLQAGPV--TVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVKAGIYKETVV 303
            R+LLQA      VKP++VVAQDGSG+YKTINEAL  +P+K N TFV+++KAG+YKE V 
Sbjct: 246 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 305

Query: 304 ITNAMTHVTMYGDGPTKTVVTGSLNYIDGVQTFKTATFATIGTNFFARDMGFENSAGPAK 363
           +   M+H+   GDGP KT+++G+ NY DG+ T++TAT A +G  F A+++GFEN+AG  K
Sbjct: 306 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 365

Query: 364 HQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFGNSAAIFQNCK 423
           HQAVA+RVQSD SIF+NCR DGYQDTLY  +HR F+RDC I+GTIDF+FG++AA+FQNC 
Sbjct: 366 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 425

Query: 424 LVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKAYLGRPWKAYS 483
           L+VRKPL NQ C +TAHGR   RE T  VFQGC    +P Y   +  +KAYLGRPWK YS
Sbjct: 426 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 485

Query: 484 RTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKWRGIKEITPKH 543
           RTIIM + I D +QP+GW PW+G+FGL TLFY+E+QNTGPG+  + RV W GIK ++ + 
Sbjct: 486 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 545

Query: 544 AVDFTPRPFIDGDAWIKPKGIPYSSYMM 570
            + FTP  +I GD WI  KG+PY++ ++
Sbjct: 546 ILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of Moc07g26900 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 559.3 bits (1440), Expect = 3.6e-159
Identity = 273/573 (47.64%), Postives = 383/573 (66.84%), Query Frame = 0

Query: 3   GDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKS 62
           G  G+ KKKK  +  V   +LV MV  VAVG++   +  H     +E     QI T+T +
Sbjct: 2   GVDGELKKKKCIIAGVITALLVLMV--VAVGITTSRNTSH-----SEKIVPVQIKTATTA 61

Query: 63  IKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQNSTT--LKELAK 122
           ++++C PTDYK+ C  SL      +T P +LIK  F +    I  +I+ ++     + A 
Sbjct: 62  VEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAN 121

Query: 123 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 182
           D     AL+ C +L+  A DD+K+  +    F + ++++F++DL++WL+G++ Y+ TC+D
Sbjct: 122 DKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMD 181

Query: 183 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIP----TLG----RRLMA 242
            FE T  +  + M++  K+S++LTSN LAM+T ++N LG   +      LG    + L A
Sbjct: 182 TFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA 241

Query: 243 ENDAPEWVSDTNRRLLQAGPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVIYVK 302
           E+  P WV    RRL+ A    VK NVVVA DGSG+YKTINEAL  +P+ +   FVIY+K
Sbjct: 242 EDGIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIK 301

Query: 303 AGIYKETVVITNAMTHVTMYGDGPTKTVVTGSLNYIDG-VQTFKTATFATIGTNFFARDM 362
            G+Y E V +T  MTHVT  GDGPTKT +TGSLNY  G V+T+ TAT A  G NF A+++
Sbjct: 302 QGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNI 361

Query: 363 GFENSAGPAKHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDFVFG 422
           GFEN+AGP  HQAVALRV +D ++FYNC++DGYQDTLY  +HR F+RDC ++GT+DF+FG
Sbjct: 362 GFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFG 421

Query: 423 NSAAIFQNCKLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAVNKA 482
           +   + QNC +VVRKP+ +Q C++TA GR  KRE T LV Q CH T +P Y P +++NKA
Sbjct: 422 DGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKA 481

Query: 483 YLGRPWKAYSRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKRVKW 542
           YLGRPWK +SRTIIM + IDD+I P GWLPW G+F LNTL+YAE +N GPG+++++RVKW
Sbjct: 482 YLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKW 541

Query: 543 RGIKEITPKHAVDFTPRPFIDGDAWIKPKGIPY 565
            GIK+++PK A+ FTP  F+ G+ WI P  +PY
Sbjct: 542 PGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of Moc07g26900 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 528.1 bits (1359), Expect = 8.8e-150
Identity = 271/578 (46.89%), Postives = 383/578 (66.26%), Query Frame = 0

Query: 3   GDAGDQKKKKIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKS 62
           G  GD+KKK I   SVS   LV MVV+VAV  S          H  + D+ H I  +TK+
Sbjct: 2   GSDGDKKKKFIVAGSVSG-FLVIMVVSVAVVTSK---------HSPKDDENH-IRKTTKA 61

Query: 63  IKSICQPTDYKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAIQN-STTLKELA-K 122
           ++++C PTD+KD C  SL      + DP +LIK  FK+    IN +++  S  +K  A K
Sbjct: 62  VQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADK 121

Query: 123 DPRANQALQNCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLD 182
           +P A  A + C +L+  AIDD+K+  +    F V +++ F++DL++WL+G++ ++ TC+D
Sbjct: 122 NPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMD 181

Query: 183 GFENTTGEAGEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIPTL-------GRRLMAE 242
            F        + M +  K+S++L+SNSLAMVT ++  + N  +  L        R+L++ 
Sbjct: 182 SFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST 241

Query: 243 NDA-PEWVSDTNRRLLQA---GPVTVKPNVVVAQDGSGKYKTINEALVEIPQKSNVTFVI 302
            D+ P WV    RRL+ A   GP  VK N VVAQDG+G++KTI +AL  +P+ + V F+I
Sbjct: 242 EDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFII 301

Query: 303 YVKAGIYKETVVITNAMTHVTMYGDGPTKTVVTGSLNY-IDGVQTFKTATFATIGTNFFA 362
           ++K GIYKE V +T  M HVT  GDGP KT++TGSLN+ I  V+TF TAT    G +F A
Sbjct: 302 HIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTA 361

Query: 363 RDMGFENSAGPAKHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDCVITGTIDF 422
           +++G EN+AGP   QAVALRV +D ++F++C++DG+QDTLY  +HR FYRDC ++GT+DF
Sbjct: 362 KNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDF 421

Query: 423 VFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDPTYYPHRAV 482
           +FG++  I QNCK+VVRKP   Q C+VTA GR   RE T LV  GCH T DP Y P ++V
Sbjct: 422 IFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSV 481

Query: 483 NKAYLGRPWKAYSRTIIMQSQIDDLIQPEGWLPWMGNFGLNTLFYAELQNTGPGADKSKR 542
           NKAYLGRPWK +SRTIIM++ IDD+I P GWLPW G+F L TL+YAE  NTGPG+++++R
Sbjct: 482 NKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQR 541

Query: 543 VKWRGIKEITPKHAVDFTPRPFIDGDAWIKPKGIPYSS 567
           VKW GIK++TP+ A+ +T   F+ GD WI    +PY++
Sbjct: 542 VKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Moc07g26900 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 448.4 bits (1152), Expect = 8.8e-126
Identity = 266/706 (37.68%), Postives = 368/706 (52.12%), Query Frame = 0

Query: 12  KIAVVSVSALILVAMVVAVAVGVSNPHDVDHDDGHDTESDDGHQITTSTKSIKSICQPTD 71
           K  ++ V+ALI+ AMV+ V     N      ++ H    +    IT +T SI  IC  TD
Sbjct: 3   KYVLLGVTALIM-AMVICVEANDGNSPSRKMEEVH---RESKLMITKTTVSI--ICASTD 62

Query: 72  YKDVCEESLATEGVTATDPKELIKSAFKIATNHINAAI-QNSTTLKELA-KDPRANQALQ 131
           YK  C  SLAT  V + DP+ LI+SAF +A   I + I +    LK  A  D    +AL 
Sbjct: 63  YKQDCTTSLAT--VRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALN 122

Query: 132 NCRELLEYAIDDIKESFERIGTFEVGKVKEFIDDLKIWLTGALTYEDTCLDGFENTTGEA 191
            CREL++ AIDD++++ ++   F   ++ +F++DL +WL+G++TY+ TC+DGFE    EA
Sbjct: 123 TCRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEA 182

Query: 192 GEKMRQFLKSSQQLTSNSLAMVTELANSLGNLQIP------------TLG---------- 251
              M + ++  Q LTSN LA+   L   L   +IP             LG          
Sbjct: 183 AVMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVG 242

Query: 252 ------------------------------------------------------------ 311
                                                                       
Sbjct: 243 SSQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPK 302

Query: 312 ------------------------------------------------------------ 371
                                                                       
Sbjct: 303 KSAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLD 362

Query: 372 ----RRLMAENDAPEWVSDTNRRLLQAGPVT--VKPNVVVAQDGSGKYKTINEALVEIPQ 431
               R L  E + P WV+  +RRLL   P    +K NVVVA+DGSGK KTI +AL  +P 
Sbjct: 363 SLKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPM 422

Query: 432 KSNVTFVIYVKAGIYKETVVITNAMTHVTMYGDGPTKTVVTGSLNYI-DGVQTFKTATFA 491
           K+   FVI++K G+YKE V +T  M HV   GDGPTKTV+TG + ++ D V T++TA+ A
Sbjct: 423 KNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVA 482

Query: 492 TIGTNFFARDMGFENSAGPAKHQAVALRVQSDRSIFYNCRMDGYQDTLYAQTHRHFYRDC 551
             G  F A+D+GFEN+AG A+HQAVALRV +D ++F+NC M+GYQDTLY  THR FYR+C
Sbjct: 483 VNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNC 542

Query: 552 VITGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLGKREPTALVFQGCHFTSDP 567
            ++GTIDFVFG++ A+FQNC+ V+R+P+++QQCIVTA GR  +RE T +V      T D 
Sbjct: 543 RVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDA 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138440.10.0e+00100.00putative pectinesterase/pectinesterase inhibitor 28 [Momordica charantia][more]
XP_022138455.15.1e-27783.30putative pectinesterase/pectinesterase inhibitor 28 isoform X1 [Momordica charan... [more]
XP_022138456.14.7e-27081.02putative pectinesterase/pectinesterase inhibitor 28 isoform X2 [Momordica charan... [more]
XP_038875843.11.5e-24773.19probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida][more]
XP_004150503.11.5e-24473.29probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.... [more]
Match NameE-valueIdentityDescription
Q3E8Z83.3e-17853.12Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX869.1e-17650.35Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ215.0e-15847.64Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA11.2e-14846.89Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
Q429203.1e-13151.12Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C9G40.0e+00100.00Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009613 PE=3 SV=1[more]
A0A6J1C9H62.5e-27783.30Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009624 PE=3 SV=1[more]
A0A6J1C9S12.3e-27081.02Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009624 PE=3 SV=1[more]
A0A0A0KD247.3e-24573.29Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1[more]
A0A1S3C7B61.4e-24372.31Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27870.12.4e-17953.12Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.16.5e-17750.35Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.13.6e-15947.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.18.8e-15046.89Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.18.8e-12637.68Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 58..210
e-value: 3.5E-41
score: 152.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 62..210
e-value: 1.4E-28
score: 100.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 59..214
e-value: 1.1E-26
score: 91.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 258..554
e-value: 2.2E-136
score: 453.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 57..216
e-value: 1.8E-36
score: 127.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 61..214
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 252..568
e-value: 1.6E-116
score: 391.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..57
NoneNo IPR availablePANTHERPTHR31707:SF337PECTINESTERASEcoord: 13..566
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 13..566
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 65..216
e-value: 2.19112E-47
score: 160.682
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 402..411
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 283..302
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 254..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc07g26900.1Moc07g26900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity