Moc07g25300 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g25300
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	calmodulin-like protein 8
Location	chr7: 18868892 .. 18869696 (+)
RNA-Seq Expression	Moc07g25300
Synteny	Moc07g25300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAAAATCATGGGGGAAGGTCTGGCCAAAGAACAAATGGATGAGCTTGAAGAAGCTTTTTGTCTATTTGACAAGAACAGAGATGGTGGGTCAATTTCTTTTTCTTCTACAACCAACAATATCATAGTTTTCAATTCAATTCTGTTTTGAATATAGCTCAAATCAAACCATGTCTTGAATAATAATCAGGTTGCATCACCCCTGATGAACTAAGAACAGTGATGATCCGACAATTGGGTCTGAATCCGACAGAACAAGAACTCAAAGACATGATCAGAGAAGTTGATGCTGATGGAAATGGAACCATTGAGTTCGGGGAATTCCTCACCTTGATGTCCAAAATCATGAAGGTCTTTCATTTCCTTTCACCCCTTACTTTATTTTCCATTTTTCCTTTAGTTGAAGTAACGATTTAGTTCTTATACCTTTCGATTTTGTTTGTTAGGAAGAAGCGGAGGAGAAGCTGAAAGAGGCTTTCAAAGTGTTTGACAAGAACCAAGATGGGTATATATCAGCAAACGAGGTACAAGTGCTTGTTTTAATGGAAAAACATGAACAAAGGTCAAATTTTTTCCAATTTTTGAGGAAACCCATCAAAGAATTGATCCAAATATCATGAGATTTCTCTTGTGGTTTGCAGTTGAGCCATGTCCATGTGATGCTTAATCTTGGGGAGAAGTTAACAGAAGAGGAAGTTGTTCAGATGATCAGAGAGGCTGATTTGGATGGAGATGGCCGAGTTAACTATGATGAATTCGTCAAGATAATGACTGAGGGATGGAAAAAAACAATGTTTGCTTAG

mRNA sequence

ATGGACAAAATCATGGGGGAAGGTCTGGCCAAAGAACAAATGGATGAGCTTGAAGAAGCTTTTTGTCTATTTGACAAGAACAGAGATGGTTGCATCACCCCTGATGAACTAAGAACAGTGATGATCCGACAATTGGGTCTGAATCCGACAGAACAAGAACTCAAAGACATGATCAGAGAAGTTGATGCTGATGGAAATGGAACCATTGAGTTCGGGGAATTCCTCACCTTGATGTCCAAAATCATGAAGGAAGAAGCGGAGGAGAAGCTGAAAGAGGCTTTCAAAGTGTTTGACAAGAACCAAGATGGGTATATATCAGCAAACGAGTTGAGCCATGTCCATGTGATGCTTAATCTTGGGGAGAAGTTAACAGAAGAGGAAGTTGTTCAGATGATCAGAGAGGCTGATTTGGATGGAGATGGCCGAGTTAACTATGATGAATTCGTCAAGATAATGACTGAGGGATGGAAAAAAACAATGTTTGCTTAG

Coding sequence (CDS)

ATGGACAAAATCATGGGGGAAGGTCTGGCCAAAGAACAAATGGATGAGCTTGAAGAAGCTTTTTGTCTATTTGACAAGAACAGAGATGGTTGCATCACCCCTGATGAACTAAGAACAGTGATGATCCGACAATTGGGTCTGAATCCGACAGAACAAGAACTCAAAGACATGATCAGAGAAGTTGATGCTGATGGAAATGGAACCATTGAGTTCGGGGAATTCCTCACCTTGATGTCCAAAATCATGAAGGAAGAAGCGGAGGAGAAGCTGAAAGAGGCTTTCAAAGTGTTTGACAAGAACCAAGATGGGTATATATCAGCAAACGAGTTGAGCCATGTCCATGTGATGCTTAATCTTGGGGAGAAGTTAACAGAAGAGGAAGTTGTTCAGATGATCAGAGAGGCTGATTTGGATGGAGATGGCCGAGTTAACTATGATGAATTCGTCAAGATAATGACTGAGGGATGGAAAAAAACAATGTTTGCTTAG

Protein sequence

MDKIMGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDADGNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLTEEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKKTMFA

Homology

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BLAST of Moc07g25300 vs. NCBI nr
Match: XP_022138814.1 (calmodulin-like protein 8 [Momordica charantia])

HSP 1 Score: 325.9 bits (834), Expect = 2.0e-85
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0


Query: 1   MDKIMGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIRE 60
           MDKIMGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIRE
Sbjct: 1   MDKIMGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIRE 60

Query: 61  VDADGNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLG 120
           VDADGNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLG
Sbjct: 61  VDADGNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLG 120

Query: 121 EKLTEEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKKTMFA 163
           EKLTEEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKKTMFA
Sbjct: 121 EKLTEEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKKTMFA 162

[Close]

BLAST of Moc07g25300 vs. NCBI nr
Match: XP_023548669.1 (calmodulin-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 244.2 bits (622), Expect = 7.7e-61
Identity = 124/150 (82.67%), Postives = 136/150 (90.67%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGE L KEQMDEL EAF LFDKNRDGCIT DELRT  I++LG NPTE+ELKDMIREVDAD
Sbjct: 3   MGEALTKEQMDELREAFLLFDKNRDGCITLDELRT-EIKKLGQNPTEEELKDMIREVDAD 62

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLT 124
           GNGTIEF EF  LMSKIMKEE E+KLK+AFKVFDKNQDGYISANEL HVH+MLNLGEKLT
Sbjct: 63  GNGTIEFWEFQILMSKIMKEETEQKLKDAFKVFDKNQDGYISANELRHVHLMLNLGEKLT 122

Query: 125 EEEVVQMIREADLDGDGRVNYDEFVKIMTE 155
           EEEV+++IREADL+GDG+V+Y EFVKIMTE
Sbjct: 123 EEEVLEIIREADLNGDGQVDYPEFVKIMTE 151

[Close]

BLAST of Moc07g25300 vs. NCBI nr
Match: KAG6574954.1 (hypothetical protein SDJN03_25593, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 241.1 bits (614), Expect = 6.6e-60
Identity = 122/150 (81.33%), Postives = 135/150 (90.00%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGE L KEQMDEL EAF LFDKNRDGCIT DELRT  I++LG NPTE+ELKDMIREVDAD
Sbjct: 3   MGEALTKEQMDELREAFLLFDKNRDGCITLDELRT-EIKRLGQNPTEEELKDMIREVDAD 62

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLT 124
           GNGTIEF EF  LMS IMKEE E+KLK+AFKVFDKNQDGYISANEL HVH+MLNLGEKLT
Sbjct: 63  GNGTIEFWEFQILMSNIMKEETEQKLKDAFKVFDKNQDGYISANELRHVHLMLNLGEKLT 122

Query: 125 EEEVVQMIREADLDGDGRVNYDEFVKIMTE 155
           EEEV+++IREADL+GDG+V+Y EFVKIMT+
Sbjct: 123 EEEVLEIIREADLNGDGQVDYPEFVKIMTD 151

[Close]

BLAST of Moc07g25300 vs. NCBI nr
Match: XP_022958889.1 (calmodulin-like [Cucurbita moschata])

HSP 1 Score: 236.9 bits (603), Expect = 1.2e-58
Identity = 120/150 (80.00%), Postives = 134/150 (89.33%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGE L KEQMDEL EAF LFDKNRDGCIT DELRT  I++LG NPTE+ELKDMIREVDAD
Sbjct: 3   MGEALTKEQMDELREAFLLFDKNRDGCITLDELRT-EIKRLGQNPTEEELKDMIREVDAD 62

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLT 124
           GNGTIEF EF  LMS IMKEE E+KLK+AFKVFDKNQDGYISANEL HVH+MLNLGEKLT
Sbjct: 63  GNGTIEFWEFQILMSNIMKEETEQKLKDAFKVFDKNQDGYISANELRHVHLMLNLGEKLT 122

Query: 125 EEEVVQMIREADLDGDGRVNYDEFVKIMTE 155
           ++EV+++I EADL+GDG+V+Y EFVKIMTE
Sbjct: 123 DKEVLEIIGEADLNGDGQVDYPEFVKIMTE 151

[Close]

BLAST of Moc07g25300 vs. NCBI nr
Match: XP_038907158.1 (calmodulin-2/4-like [Benincasa hispida])

HSP 1 Score: 229.2 bits (583), Expect = 2.6e-56
Identity = 118/148 (79.73%), Postives = 129/148 (87.16%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGEGL KEQMD+L EAF  FDKNRDGCIT DELRT  IR LG NPTE+ELK MI EVDAD
Sbjct: 38  MGEGLTKEQMDQLIEAFLFFDKNRDGCITLDELRT-EIRNLGQNPTEEELKQMISEVDAD 97

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLT 124
           GNG I+F EF  LMSKIM+EE EEKLKEAFKVFDKNQDGYISANELSHV +MLNLGEKLT
Sbjct: 98  GNGKIDFWEFQKLMSKIMEEETEEKLKEAFKVFDKNQDGYISANELSHVCLMLNLGEKLT 157

Query: 125 EEEVVQMIREADLDGDGRVNYDEFVKIM 153
           +EEV+QMIR+ADLDGDG+V+Y EFV +M
Sbjct: 158 DEEVLQMIRDADLDGDGQVDYHEFVNMM 184

[Close]

BLAST of Moc07g25300 vs. ExPASy Swiss-Prot
Match: O23320 (Calmodulin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=CML8 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.7e-50
Identity = 104/145 (71.72%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 9   LAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDADGNGT 68
           L K+Q+ E +EAFCLFDK+ DGCIT +EL TV IR L  NPTEQEL D+I E+D+D NGT
Sbjct: 6   LTKDQITEFKEAFCLFDKDGDGCITVEELATV-IRSLDQNPTEQELHDIITEIDSDSNGT 65

Query: 69  IEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLTEEE 128
           IEF EFL LM+K ++E +AEE+LKEAFKVFDK+Q+GYISA+ELS  HVM+NLGEKLT+EE
Sbjct: 66  IEFAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELS--HVMINLGEKLTDEE 125

Query: 129 VVQMIREADLDGDGRVNYDEFVKIM 153
           V QMI+EADLDGDG+VNYDEFVK+M
Sbjct: 126 VEQMIKEADLDGDGQVNYDEFVKMM 147

[Close]

BLAST of Moc07g25300 vs. ExPASy Swiss-Prot
Match: Q9LIK5 (Calmodulin-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=CML11 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.7e-50
Identity = 103/145 (71.03%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 9   LAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDADGNGT 68
           L +EQ+ E +EAFCLFDK+ DGCIT DEL TV IR L  NPTEQEL+DMI E+D+DGNGT
Sbjct: 28  LTQEQIMEFKEAFCLFDKDGDGCITADELATV-IRSLDQNPTEQELQDMITEIDSDGNGT 87

Query: 69  IEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLTEEE 128
           IEF EFL LM+  ++E +A+E+LKEAFKVFDK+Q+GYISA+EL   HVM+NLGEKLT+EE
Sbjct: 88  IEFSEFLNLMANQLQETDADEELKEAFKVFDKDQNGYISASELR--HVMINLGEKLTDEE 147

Query: 129 VVQMIREADLDGDGRVNYDEFVKIM 153
           V QMI+EADLDGDG+VNYDEFV++M
Sbjct: 148 VDQMIKEADLDGDGQVNYDEFVRMM 169

[Close]

BLAST of Moc07g25300 vs. ExPASy Swiss-Prot
Match: P0DH95 (Calmodulin-1 OS=Arabidopsis thaliana OX=3702 GN=CAM1 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.1e-48
Identity = 102/149 (68.46%), Postives = 123/149 (82.55%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           M + L  EQ+ E +EAF LFDK+ DGCIT  EL TVM R LG NPTE EL+DMI EVDAD
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVM-RSLGQNPTEAELQDMINEVDAD 60

Query: 65  GNGTIEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKL 124
           GNGTI+F EFL LM+K MK+ ++EE+LKEAF+VFDK+Q+G+ISA EL   HVM NLGEKL
Sbjct: 61  GNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELR--HVMTNLGEKL 120

Query: 125 TEEEVVQMIREADLDGDGRVNYDEFVKIM 153
           T+EEV +MIREAD+DGDG++NY+EFVKIM
Sbjct: 121 TDEEVEEMIREADVDGDGQINYEEFVKIM 146

[Close]

BLAST of Moc07g25300 vs. ExPASy Swiss-Prot
Match: P0DH96 (Calmodulin-4 OS=Arabidopsis thaliana OX=3702 GN=CAM4 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.1e-48
Identity = 102/149 (68.46%), Postives = 123/149 (82.55%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           M + L  EQ+ E +EAF LFDK+ DGCIT  EL TVM R LG NPTE EL+DMI EVDAD
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVM-RSLGQNPTEAELQDMINEVDAD 60

Query: 65  GNGTIEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKL 124
           GNGTI+F EFL LM+K MK+ ++EE+LKEAF+VFDK+Q+G+ISA EL   HVM NLGEKL
Sbjct: 61  GNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELR--HVMTNLGEKL 120

Query: 125 TEEEVVQMIREADLDGDGRVNYDEFVKIM 153
           T+EEV +MIREAD+DGDG++NY+EFVKIM
Sbjct: 121 TDEEVEEMIREADVDGDGQINYEEFVKIM 146

[Close]

BLAST of Moc07g25300 vs. ExPASy Swiss-Prot
Match: Q7DMN9 (Calmodulin-5/6/7/8 OS=Solanum tuberosum OX=4113 GN=PCM5 PE=1 SV=3)

HSP 1 Score: 192.6 bits (488), Expect = 3.5e-48
Identity = 100/149 (67.11%), Postives = 124/149 (83.22%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           M + L ++Q+ E +EAF LFDK+ DGCIT  EL TVM R LG NPTE EL+DMI EVDAD
Sbjct: 1   MADQLTEDQISEFKEAFSLFDKDGDGCITTKELGTVM-RSLGQNPTEAELQDMINEVDAD 60

Query: 65  GNGTIEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKL 124
           GNGTI+F EFL LM++ MK+ ++EE+LKEAF+VFDK+Q+G+ISA EL   HVM NLGEKL
Sbjct: 61  GNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELR--HVMTNLGEKL 120

Query: 125 TEEEVVQMIREADLDGDGRVNYDEFVKIM 153
           T+EEV +MIREAD+DGDG++NYDEFVK+M
Sbjct: 121 TDEEVDEMIREADVDGDGQINYDEFVKVM 146

[Close]

BLAST of Moc07g25300 vs. ExPASy TrEMBL
Match: A0A6J1CB59 (calmodulin-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111009891 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 9.8e-86
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0


Query: 1   MDKIMGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIRE 60
           MDKIMGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIRE
Sbjct: 1   MDKIMGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIRE 60

Query: 61  VDADGNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLG 120
           VDADGNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLG
Sbjct: 61  VDADGNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLG 120

Query: 121 EKLTEEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKKTMFA 163
           EKLTEEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKKTMFA
Sbjct: 121 EKLTEEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKKTMFA 162

[Close]

BLAST of Moc07g25300 vs. ExPASy TrEMBL
Match: A0A6J1H6E9 (calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460040 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.0e-59
Identity = 120/150 (80.00%), Postives = 134/150 (89.33%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGE L KEQMDEL EAF LFDKNRDGCIT DELRT  I++LG NPTE+ELKDMIREVDAD
Sbjct: 3   MGEALTKEQMDELREAFLLFDKNRDGCITLDELRT-EIKRLGQNPTEEELKDMIREVDAD 62

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLT 124
           GNGTIEF EF  LMS IMKEE E+KLK+AFKVFDKNQDGYISANEL HVH+MLNLGEKLT
Sbjct: 63  GNGTIEFWEFQILMSNIMKEETEQKLKDAFKVFDKNQDGYISANELRHVHLMLNLGEKLT 122

Query: 125 EEEVVQMIREADLDGDGRVNYDEFVKIMTE 155
           ++EV+++I EADL+GDG+V+Y EFVKIMTE
Sbjct: 123 DKEVLEIIGEADLNGDGQVDYPEFVKIMTE 151

[Close]

BLAST of Moc07g25300 vs. ExPASy TrEMBL
Match: A0A0A0LLM7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G215510 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.1e-55
Identity = 119/151 (78.81%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGE L KEQMD+L+EAF LFDKN+DG IT DELR   IR LG NPTE+ELK+MIREVDAD
Sbjct: 1   MGEALTKEQMDQLKEAFFLFDKNKDGRITIDELR-AEIRNLGHNPTEEELKEMIREVDAD 60

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLG-EKL 124
           GNGTIEFGEF  LMSKIMKEE E+KLKEAFKVFDKNQDGYISANELSHV+ MLN G EKL
Sbjct: 61  GNGTIEFGEFQNLMSKIMKEETEKKLKEAFKVFDKNQDGYISANELSHVYWMLNSGEEKL 120

Query: 125 TEEEVVQMIREADLDGDGRVNYDEFVKIMTE 155
           T+EEV  MI EADL+GDG V+Y EFVKIMT+
Sbjct: 121 TDEEVFHMISEADLNGDGHVDYHEFVKIMTQ 150

[Close]

BLAST of Moc07g25300 vs. ExPASy TrEMBL
Match: A0A6J1KG52 (calmodulin-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111494791 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-52
Identity = 110/154 (71.43%), Postives = 127/154 (82.47%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGEGL ++QMD+L+E F LFD+N DGCIT DELRT  I++   N TE+ELKDMI EVDAD
Sbjct: 3   MGEGLTRQQMDQLQEVFHLFDRNGDGCITLDELRT-EIQKFDYNLTEEELKDMINEVDAD 62

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLT 124
           GNGTIEFGE   LMSK  KEE EEKL+EAFK+FD+NQDGYISANELS V  MLNLGE+LT
Sbjct: 63  GNGTIEFGELRNLMSKTFKEETEEKLEEAFKLFDENQDGYISANELSSVLRMLNLGERLT 122

Query: 125 EEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKK 159
            EE+ QMI +ADLDGDG+V+Y EFV +MTE WKK
Sbjct: 123 YEEIQQMINDADLDGDGQVDYHEFVNMMTEYWKK 155

[Close]

BLAST of Moc07g25300 vs. ExPASy TrEMBL
Match: A0A6J1EWT4 (calmodulin-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111436855 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-52
Identity = 110/154 (71.43%), Postives = 126/154 (81.82%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           MGEGL ++QMD+L+E F LFD+N DGCIT DELRT  I++   N TE+ELKDMI EVDAD
Sbjct: 3   MGEGLTRQQMDQLQEVFHLFDRNGDGCITLDELRT-EIQKFDYNLTEEELKDMINEVDAD 62

Query: 65  GNGTIEFGEFLTLMSKIMKEEAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLT 124
           GNGTIEFGE   LMSK  KEE EEKL+EAFK+FD+NQDGYISANELS V  MLNLGE+LT
Sbjct: 63  GNGTIEFGELRNLMSKTFKEETEEKLEEAFKLFDENQDGYISANELSSVLRMLNLGERLT 122

Query: 125 EEEVVQMIREADLDGDGRVNYDEFVKIMTEGWKK 159
            EE+ QMI +ADLDGDG V+Y EFV +MTE WKK
Sbjct: 123 YEEIQQMINDADLDGDGHVDYHEFVNMMTEYWKK 155

[Close]

BLAST of Moc07g25300 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 200.3 bits (508), Expect = 1.2e-51
Identity = 104/145 (71.72%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 9   LAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDADGNGT 68
           L K+Q+ E +EAFCLFDK+ DGCIT +EL TV IR L  NPTEQEL D+I E+D+D NGT
Sbjct: 6   LTKDQITEFKEAFCLFDKDGDGCITVEELATV-IRSLDQNPTEQELHDIITEIDSDSNGT 65

Query: 69  IEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLTEEE 128
           IEF EFL LM+K ++E +AEE+LKEAFKVFDK+Q+GYISA+ELS  HVM+NLGEKLT+EE
Sbjct: 66  IEFAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELS--HVMINLGEKLTDEE 125

Query: 129 VVQMIREADLDGDGRVNYDEFVKIM 153
           V QMI+EADLDGDG+VNYDEFVK+M
Sbjct: 126 VEQMIKEADLDGDGQVNYDEFVKMM 147

[Close]

BLAST of Moc07g25300 vs. TAIR 10
Match: AT3G22930.1 (calmodulin-like 11 )

HSP 1 Score: 199.1 bits (505), Expect = 2.7e-51
Identity = 103/145 (71.03%), Postives = 125/145 (86.21%), Query Frame = 0


Query: 9   LAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDADGNGT 68
           L +EQ+ E +EAFCLFDK+ DGCIT DEL TV IR L  NPTEQEL+DMI E+D+DGNGT
Sbjct: 28  LTQEQIMEFKEAFCLFDKDGDGCITADELATV-IRSLDQNPTEQELQDMITEIDSDGNGT 87

Query: 69  IEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKLTEEE 128
           IEF EFL LM+  ++E +A+E+LKEAFKVFDK+Q+GYISA+EL   HVM+NLGEKLT+EE
Sbjct: 88  IEFSEFLNLMANQLQETDADEELKEAFKVFDKDQNGYISASELR--HVMINLGEKLTDEE 147

Query: 129 VVQMIREADLDGDGRVNYDEFVKIM 153
           V QMI+EADLDGDG+VNYDEFV++M
Sbjct: 148 VDQMIKEADLDGDGQVNYDEFVRMM 169

[Close]

BLAST of Moc07g25300 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 193.4 bits (490), Expect = 1.5e-49
Identity = 102/149 (68.46%), Postives = 123/149 (82.55%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           M + L  EQ+ E +EAF LFDK+ DGCIT  EL TVM R LG NPTE EL+DMI EVDAD
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVM-RSLGQNPTEAELQDMINEVDAD 60

Query: 65  GNGTIEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKL 124
           GNGTI+F EFL LM+K MK+ ++EE+LKEAF+VFDK+Q+G+ISA EL   HVM NLGEKL
Sbjct: 61  GNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELR--HVMTNLGEKL 120

Query: 125 TEEEVVQMIREADLDGDGRVNYDEFVKIM 153
           T+EEV +MIREAD+DGDG++NY+EFVKIM
Sbjct: 121 TDEEVEEMIREADVDGDGQINYEEFVKIM 146

[Close]

BLAST of Moc07g25300 vs. TAIR 10
Match: AT5G37780.1 (calmodulin 1 )

HSP 1 Score: 193.4 bits (490), Expect = 1.5e-49
Identity = 102/149 (68.46%), Postives = 123/149 (82.55%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           M + L  EQ+ E +EAF LFDK+ DGCIT  EL TVM R LG NPTE EL+DMI EVDAD
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVM-RSLGQNPTEAELQDMINEVDAD 60

Query: 65  GNGTIEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKL 124
           GNGTI+F EFL LM+K MK+ ++EE+LKEAF+VFDK+Q+G+ISA EL   HVM NLGEKL
Sbjct: 61  GNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELR--HVMTNLGEKL 120

Query: 125 TEEEVVQMIREADLDGDGRVNYDEFVKIM 153
           T+EEV +MIREAD+DGDG++NY+EFVKIM
Sbjct: 121 TDEEVEEMIREADVDGDGQINYEEFVKIM 146

[Close]

BLAST of Moc07g25300 vs. TAIR 10
Match: AT3G43810.1 (calmodulin 7 )

HSP 1 Score: 190.3 bits (482), Expect = 1.2e-48
Identity = 99/149 (66.44%), Postives = 123/149 (82.55%), Query Frame = 0


Query: 5   MGEGLAKEQMDELEEAFCLFDKNRDGCITPDELRTVMIRQLGLNPTEQELKDMIREVDAD 64
           M + L  +Q+ E +EAF LFDK+ DGCIT  EL TVM R LG NPTE EL+DMI EVDAD
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVM-RSLGQNPTEAELQDMINEVDAD 60

Query: 65  GNGTIEFGEFLTLMSKIMKE-EAEEKLKEAFKVFDKNQDGYISANELSHVHVMLNLGEKL 124
           GNGTI+F EFL LM++ MK+ ++EE+LKEAF+VFDK+Q+G+ISA EL   HVM NLGEKL
Sbjct: 61  GNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELR--HVMTNLGEKL 120

Query: 125 TEEEVVQMIREADLDGDGRVNYDEFVKIM 153
           T+EEV +MIREAD+DGDG++NY+EFVK+M
Sbjct: 121 TDEEVDEMIREADVDGDGQINYEEFVKVM 146

The following BLAST results are available for this feature:

BLAST of Moc07g25300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138814.1	2.0e-85	100.00	calmodulin-like protein 8 [Momordica charantia]	[more]
XP_023548669.1	7.7e-61	82.67	calmodulin-1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6574954.1	6.6e-60	81.33	hypothetical protein SDJN03_25593, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022958889.1	1.2e-58	80.00	calmodulin-like [Cucurbita moschata]	[more]
XP_038907158.1	2.6e-56	79.73	calmodulin-2/4-like [Benincasa hispida]	[more]

BLAST of Moc07g25300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23320	1.7e-50	71.72	Calmodulin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=CML8 PE=1 SV=1	[more]
Q9LIK5	3.7e-50	71.03	Calmodulin-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=CML11 PE=2 SV=1	[more]
P0DH95	2.1e-48	68.46	Calmodulin-1 OS=Arabidopsis thaliana OX=3702 GN=CAM1 PE=1 SV=1	[more]
P0DH96	2.1e-48	68.46	Calmodulin-4 OS=Arabidopsis thaliana OX=3702 GN=CAM4 PE=1 SV=1	[more]
Q7DMN9	3.5e-48	67.11	Calmodulin-5/6/7/8 OS=Solanum tuberosum OX=4113 GN=PCM5 PE=1 SV=3	[more]

BLAST of Moc07g25300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CB59	9.8e-86	100.00	calmodulin-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111009891 PE=4 SV...	[more]
A0A6J1H6E9	6.0e-59	80.00	calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460040 PE=4 SV=1	[more]
A0A0A0LLM7	3.1e-55	78.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G215510 PE=4 SV=1	[more]
A0A6J1KG52	1.1e-52	71.43	calmodulin-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111494791 PE=4 SV=1	[more]
A0A6J1EWT4	1.4e-52	71.43	calmodulin-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111436855 PE=4 SV=...	[more]

BLAST of Moc07g25300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14640.1	1.2e-51	71.72	calmodulin 8	[more]
AT3G22930.1	2.7e-51	71.03	calmodulin-like 11	[more]
AT1G66410.1	1.5e-49	68.46	calmodulin 4	[more]
AT5G37780.1	1.5e-49	68.46	calmodulin 1	[more]
AT3G43810.1	1.2e-48	66.44	calmodulin 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 16..44 e-value: 5.3E-6 score: 36.0 coord: 127..155 e-value: 3.3E-6 score: 36.6 coord: 89..117 e-value: 1.3E-4 score: 31.3 coord: 53..81 e-value: 2.3E-8 score: 43.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 87..152 e-value: 1.1E-18 score: 67.5 coord: 15..79 e-value: 3.8E-14 score: 52.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 85..120 score: 14.987627
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 49..84 score: 14.84815
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 12..47 score: 14.84815
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 123..158 score: 13.341799
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 16..79 e-value: 3.83491E-18 score: 71.8101
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 94..153 e-value: 1.79157E-17 score: 70.2693
None	No IPR available	GENE3D	1.10.238.10		coord: 82..161 e-value: 5.2E-28 score: 99.3
None	No IPR available	GENE3D	1.10.238.10		coord: 1..81 e-value: 1.6E-28 score: 100.9
None	No IPR available	PANTHER	PTHR23050:SF435	CALMODULIN-LIKE PROTEIN 8	coord: 7..154
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 7..154
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 25..37
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 98..110
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 62..74
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 136..148
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 11..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g25300.1	Moc07g25300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)+] activity
molecular_function	GO:0005509	calcium ion binding