Moc07g23090 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc07g23090
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRING-type E3 ubiquitin transferase
Locationchr7: 17046105 .. 17047694 (-)
RNA-Seq ExpressionMoc07g23090
SyntenyMoc07g23090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAAATCCACCAAAACCCTCATCCTCCGCCGTGGCCGCCACCGCCGGCCGCCGTTCCTCCATATTTCCGGTGCCCTATTTCCATGGAAGTGATGCAAGATCCGGTCACCATTTCCACCGGCGTCACCTTCGAAAGAGCCAACATAGAGAGGTGGTTCTTCACTTACAACAAGAGAACTTGCCCTGCCACCATGCAAACCATCCTCAACTTTGATATCACCCCTAATTATACCCTCAAGAGGCTAATACTTTCTTGGAATATGAAAGAATCTTGCTCGTCGTCATCGTCGTCGTCATCGTTGGTGGAGTACGACGATGTCGGGGCTCTCTTGAAGAACGTCGAGTCGTCGCCATTCAAGGTGAGTTCTTTGAGGAAACTACGACTGCTCGCGGCGGTGGATGATGTTGCTGCTAATGGAGTGAAATCCGTCTTTTCTCGAGCAAATGGGTTTGAAGTTCTGGTCGGAATAGTCAATCAAGTCGTTGCCTCTGAAAGCTTCGATTTTGCTAACTTCGAAGCTTGTCAGGAGGCTTTAGGGGTAATATGGCTTAAGTTGATAGATTAGGATGTTTTTTAATCTTTTATTTATATACTTAACACTCTCCCCTCTCGACCTTGAGAAATTTAGCACAAATTTAGCACAAGAGACGTGTAGAGTTTGTTTAATTTTTTATTTTATTATTACTAGGTATTATCTCACTTTTCATTTTCCAAAAGAGATGAGAAGTTATTTGAGATGCTATCCAAACCCGAATCCATCAAATCAATGGCGGTTGTTTTACAAAGAGGAAGCATAGAAGGACGGTTTTACGCAATGGAAATGCTTCAAAAGATGTGCCAAAACGGCTACGATTGGAACTTGCTAACTAGATATCAAGCCATTGACTTGTTTAAATCGATCTTCGAGCTCGCATCAGATGAGATGAACAAGCAAGCAAGTACCCTTATGTTAAGGGATTTCTCTACAATTCCAAAATGGTATGATTATCCACTTTGGACTTAAAGCCACACATGATGCGTAGATCCCTTTTTTAAGTTTCACCCTAAAAGAATGGAGAGATATTATCTTACCTCCTTAATCTTTTCATTTTCTAGTCGATGTGAAACATTGTTCTCCTCCACCCTAAAGCTTTTGATAGAGATCATGGAGTCCTCGAAGAAGAGTAGGTTGAGATCGATCGAAGCCGGTGCCGTCTGCATCATGATCGACCTCCTCCCAGACTTGAACCGATCGAAGTGCGAGAGAACATTACACATACTCAAACTTCTGAGCGAGTGCGCAGAGGGCAGGTCGGCCATTGCGGAGCACGCCATGGGAATAGCAATAGTGACGAAGAAAATGAGTGCGTCGAACGCAGCGACAAAAATCGGGGTGAAGATCTTGTGGTTGATGTGCAACTATCATCCTGGCGAGAGGGTTTTGGAGGAGATGATGGGGTGTGGGGCAGTGAAGAAGCTTTTGGGGATCTTGAATAATAATGGCGATGGCAGATCTTCAACAAAGGAGAAGGCAAGGAAGATCATGAACATTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGGGATTAA

mRNA sequence

ATGGAGGAAATCCACCAAAACCCTCATCCTCCGCCGTGGCCGCCACCGCCGGCCGCCGTTCCTCCATATTTCCGGTGCCCTATTTCCATGGAAGTGATGCAAGATCCGGTCACCATTTCCACCGGCGTCACCTTCGAAAGAGCCAACATAGAGAGGTGGTTCTTCACTTACAACAAGAGAACTTGCCCTGCCACCATGCAAACCATCCTCAACTTTGATATCACCCCTAATTATACCCTCAAGAGGCTAATACTTTCTTGGAATATGAAAGAATCTTGCTCGTCGTCATCGTCGTCGTCATCGTTGGTGGAGTACGACGATGTCGGGGCTCTCTTGAAGAACGTCGAGTCGTCGCCATTCAAGGTGAGTTCTTTGAGGAAACTACGACTGCTCGCGGCGGTGGATGATGTTGCTGCTAATGGAGTGAAATCCGTCTTTTCTCGAGCAAATGGGTTTGAAGTTCTGGTCGGAATAGTCAATCAAGTCGTTGCCTCTGAAAGCTTCGATTTTGCTAACTTCGAAGCTTGTCAGGAGGCTTTAGGGGTATTATCTCACTTTTCATTTTCCAAAAGAGATGAGAAGTTATTTGAGATGCTATCCAAACCCGAATCCATCAAATCAATGGCGGTTGTTTTACAAAGAGGAAGCATAGAAGGACGGTTTTACGCAATGGAAATGCTTCAAAAGATGTGCCAAAACGGCTACGATTGGAACTTGCTAACTAGATATCAAGCCATTGACTTGTTTAAATCGATCTTCGAGCTCGCATCAGATGAGATGAACAAGCAAGCAAGTACCCTTATGTTAAGGGATTTCTCTACAATTCCAAAATGTCGATGTGAAACATTGTTCTCCTCCACCCTAAAGCTTTTGATAGAGATCATGGAGTCCTCGAAGAAGAGTAGGTTGAGATCGATCGAAGCCGGTGCCGTCTGCATCATGATCGACCTCCTCCCAGACTTGAACCGATCGAAGTGCGAGAGAACATTACACATACTCAAACTTCTGAGCGAGTGCGCAGAGGGCAGGTCGGCCATTGCGGAGCACGCCATGGGAATAGCAATAGTGACGAAGAAAATGAGTGCGTCGAACGCAGCGACAAAAATCGGGGTGAAGATCTTGTGGTTGATGTGCAACTATCATCCTGGCGAGAGGGTTTTGGAGGAGATGATGGGGTGTGGGGCAGTGAAGAAGCTTTTGGGGATCTTGAATAATAATGGCGATGGCAGATCTTCAACAAAGGAGAAGGCAAGGAAGATCATGAACATTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGGGATTAA

Coding sequence (CDS)

ATGGAGGAAATCCACCAAAACCCTCATCCTCCGCCGTGGCCGCCACCGCCGGCCGCCGTTCCTCCATATTTCCGGTGCCCTATTTCCATGGAAGTGATGCAAGATCCGGTCACCATTTCCACCGGCGTCACCTTCGAAAGAGCCAACATAGAGAGGTGGTTCTTCACTTACAACAAGAGAACTTGCCCTGCCACCATGCAAACCATCCTCAACTTTGATATCACCCCTAATTATACCCTCAAGAGGCTAATACTTTCTTGGAATATGAAAGAATCTTGCTCGTCGTCATCGTCGTCGTCATCGTTGGTGGAGTACGACGATGTCGGGGCTCTCTTGAAGAACGTCGAGTCGTCGCCATTCAAGGTGAGTTCTTTGAGGAAACTACGACTGCTCGCGGCGGTGGATGATGTTGCTGCTAATGGAGTGAAATCCGTCTTTTCTCGAGCAAATGGGTTTGAAGTTCTGGTCGGAATAGTCAATCAAGTCGTTGCCTCTGAAAGCTTCGATTTTGCTAACTTCGAAGCTTGTCAGGAGGCTTTAGGGGTATTATCTCACTTTTCATTTTCCAAAAGAGATGAGAAGTTATTTGAGATGCTATCCAAACCCGAATCCATCAAATCAATGGCGGTTGTTTTACAAAGAGGAAGCATAGAAGGACGGTTTTACGCAATGGAAATGCTTCAAAAGATGTGCCAAAACGGCTACGATTGGAACTTGCTAACTAGATATCAAGCCATTGACTTGTTTAAATCGATCTTCGAGCTCGCATCAGATGAGATGAACAAGCAAGCAAGTACCCTTATGTTAAGGGATTTCTCTACAATTCCAAAATGTCGATGTGAAACATTGTTCTCCTCCACCCTAAAGCTTTTGATAGAGATCATGGAGTCCTCGAAGAAGAGTAGGTTGAGATCGATCGAAGCCGGTGCCGTCTGCATCATGATCGACCTCCTCCCAGACTTGAACCGATCGAAGTGCGAGAGAACATTACACATACTCAAACTTCTGAGCGAGTGCGCAGAGGGCAGGTCGGCCATTGCGGAGCACGCCATGGGAATAGCAATAGTGACGAAGAAAATGAGTGCGTCGAACGCAGCGACAAAAATCGGGGTGAAGATCTTGTGGTTGATGTGCAACTATCATCCTGGCGAGAGGGTTTTGGAGGAGATGATGGGGTGTGGGGCAGTGAAGAAGCTTTTGGGGATCTTGAATAATAATGGCGATGGCAGATCTTCAACAAAGGAGAAGGCAAGGAAGATCATGAACATTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGGGATTAA

Protein sequence

MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSSSLVEYDDVGALLKNVESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKIMNIHGSFWRRFPCFPYEFRD
Homology
BLAST of Moc07g23090 vs. NCBI nr
Match: XP_022155721.1 (E3 ubiquitin-protein ligase PUB23-like [Momordica charantia])

HSP 1 Score: 870.2 bits (2247), Expect = 7.8e-249
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKR 60
           MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKR
Sbjct: 1   MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKR 60

Query: 61  TCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSSSLVEYDDVGALLKNVESSPF 120
           TCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSSSLVEYDDVGALLKNVESSPF
Sbjct: 61  TCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSSSLVEYDDVGALLKNVESSPF 120

Query: 121 KVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEAL 180
           KVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEAL
Sbjct: 121 KVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEAL 180

Query: 181 GVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLL 240
           GVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLL
Sbjct: 181 GVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLL 240

Query: 241 TRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKK 300
           TRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKK
Sbjct: 241 TRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKK 300

Query: 301 SRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKM 360
           SRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKM
Sbjct: 301 SRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKM 360

Query: 361 SASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKI 420
           SASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKI
Sbjct: 361 SASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKI 420

Query: 421 MNIHGSFWRRFPCFPYEFRD 441
           MNIHGSFWRRFPCFPYEFRD
Sbjct: 421 MNIHGSFWRRFPCFPYEFRD 440

BLAST of Moc07g23090 vs. NCBI nr
Match: XP_004145072.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical protein Csa_004999 [Cucumis sativus])

HSP 1 Score: 662.5 bits (1708), Expect = 2.5e-186
Identity = 344/446 (77.13%), Postives = 382/446 (85.65%), Query Frame = 0

Query: 1   MEEIHQNPHPPPWPPPPAAV--PPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYN 60
           ME+IHQNP PPP  P P  V  PP+FRCPISME+MQDPVTISTGV+FERANIE+WFFTYN
Sbjct: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KRTCPATMQTILNFDITPNYTLKRLILSWNMK-ESCSSSSS---SSSLVEYDDVGALLKN 120
           K+TCPATMQTI+NFDITPNYTLKRLILSW MK +SCSS+SS   SSS+  +D V  LLKN
Sbjct: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120

Query: 121 VESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFE 180
           +ESSPFK SSL+KLRLL AVDDVAA G K VF R NGFEVL+GIV QV   ES DFANFE
Sbjct: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180

Query: 181 ACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNG 240
           AC+EALGVLS F FSK  EK FE+LS+PE+IKSMA+VLQRGSIEGRFYAMEMLQ++ + G
Sbjct: 181 ACEEALGVLSQFPFSK-TEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEI 300
           YDWN LT++Q IDLFKSI ELASDE+ K+AS +   D S +  CR  TLFSS L+LLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIA 360
           MESSKKSRL SIEAGAVC+MIDLLPD NRSKCE+ LHILKLLSECAEGRSA+ EH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360

Query: 361 IVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTK 420
            VTKKMS SN ATKIG+KILWL+CNYHP ERVLEEMMGCG VKKLLGIL NNGDGRSSTK
Sbjct: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL-NNGDGRSSTK 420

Query: 421 EKARKIMNIHGSFWRRFPCFPYEFRD 441
           EKA+K+M +HGSFWRRFPCFPYE+RD
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRD 444

BLAST of Moc07g23090 vs. NCBI nr
Match: XP_038875803.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 662.1 bits (1707), Expect = 3.2e-186
Identity = 346/446 (77.58%), Postives = 384/446 (86.10%), Query Frame = 0

Query: 1   MEEIHQNPHPPPWPPPPAAV----PPYFRCPISMEVMQDPVTISTGVTFERANIERWFFT 60
           ME+IHQN  PPP PPPP+      PPYFRCPISME+MQDPVTISTGV+FERANIE+WFFT
Sbjct: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKRTCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSS-SSLVEYDDVGA-LLKN 120
           YNK+TCPATMQTI+NFDITPNYTLKRLILSW  KES SSSSSS SS ++YDDV A LLKN
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLKN 120

Query: 121 VESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFE 180
           +ES+PFK SSL+KLRLL A+DDVAA G K VF RANGF+VLVGI+ QV   ES DFANFE
Sbjct: 121 IESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFE 180

Query: 181 ACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNG 240
           AC+EALGVLS F FSK  EK FE+LS+PE+IKSMA+VLQRGS+EGRFYAMEMLQ++ +  
Sbjct: 181 ACEEALGVLSQFPFSK-TEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKE 240

Query: 241 YDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEI 300
           YDWN LT++  IDLFKSI ELASDE+ K+AST+   +FS I +CR  TLFSS L+LLIEI
Sbjct: 241 YDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIA 360
           MESSKKSRL SIEAGAVC+MIDLLPD NRSKCE+ LHILKLLSECAEGRSA+ EH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360

Query: 361 IVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTK 420
            VTKKMS SN ATKIG+KILWL+CNYHP ERVLEEMMGCG VKKLLGIL  NGDGRSSTK
Sbjct: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL--NGDGRSSTK 420

Query: 421 EKARKIMNIHGSFWRRFPCFPYEFRD 441
           EKA KIM +HGSFWRRFPCFPYEFRD
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRD 443

BLAST of Moc07g23090 vs. NCBI nr
Match: XP_008460073.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 650.6 bits (1677), Expect = 9.7e-183
Identity = 339/447 (75.84%), Postives = 380/447 (85.01%), Query Frame = 0

Query: 1   MEEIHQNPHPP---PWPPPPAAV--PPYFRCPISMEVMQDPVTISTGVTFERANIERWFF 60
           ME+IHQ+P PP   P PPP A+V  PP+FRCPISME+MQDPVTISTGV+FERANIE+WFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKRTCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSS--SSSSLVEYDDVGALLK 120
           TYNK+TCPATMQTI+NFDITPNYTLKRLILSW MKESCSS+S   SSS+  +D V  LLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NVESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANF 180
           N+ESSPFK SSL+KLRLL AVDDVAA G K VF R NGFE+LV IV QV   E  DFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQN 240
           EAC+EALGVLS F FSK  EK FE+LS+PE+IKSMA++LQRGSIEGRFYAMEMLQ++ + 
Sbjct: 181 EACEEALGVLSQFPFSK-TEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240

Query: 241 GYDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIE 300
           GYDWN LT++Q IDLFKSI ELASDE+ K+AS +   D STI +CR  TLFSS L+LLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGI 360
           IMESSKKSRL SIEAGAVC+MIDLLPD NRSKCE+ L+ILKLLSECAEGRSA+ EH MGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGI 360

Query: 361 AIVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSST 420
           A VTKKMS SN ATKIG+KILW +C+YHP ERVLEEMMGCG VKKLLGIL  NGDG SST
Sbjct: 361 ATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGIL--NGDGSSST 420

Query: 421 KEKARKIMNIHGSFWRRFPCFPYEFRD 441
           KEKA K+M +HGSFWRRFPCFPYE+RD
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRD 444

BLAST of Moc07g23090 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649.8 bits (1675), Expect = 1.7e-182
Identity = 341/448 (76.12%), Postives = 381/448 (85.04%), Query Frame = 0

Query: 1   MEEIHQNPHPP--PWPPPPAA--VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFT 60
           ME+I QN  PP  P PPPPA   +PPYFRCPISMEVM DPVTISTGV+FERANIE+WFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKRTCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSS---SLVEYDDVGALLK 120
           YNK+TCPATMQ I+NFDITPNYTLKRLILSW M ESCSS+SSSS     +EYDDV ALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120

Query: 121 NVESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANF 180
           N+E SPFK SSL+KLRLL A+DDVAA G K +F +ANGFEVLVG+V QV   ES +FANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQN 240
           EAC+EALGVLS F FSK  EKLFE+LS+PE+IKSMA+VLQRGSIEGRFYAME+LQ++ + 
Sbjct: 181 EACEEALGVLSQFPFSK-TEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIE 300
           GYD+N LT++ AIDLFKSI ELASDE+ K+AST+ + D S   +CR  TLFSS L+LLIE
Sbjct: 241 GYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGI 360
           IMESSKK RL  IEAGAVCIMIDLLPD NRSKCE+ LH+LKLLS+CAEGR A+AEH MGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 AIVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGR-SS 420
           A VTKKMS SN ATKIGVKILWL+CNYHP ERVLEEMMGCG VKKL+GIL  NGDGR SS
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGIL--NGDGRSSS 420

Query: 421 TKEKARKIMNIHGSFWRRFPCFPYEFRD 441
           TKEKA KIM +HGSFWRRFPCFPYEFRD
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRD 445

BLAST of Moc07g23090 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.1e-43
Identity = 129/426 (30.28%), Postives = 213/426 (50.00%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           +PP+F CPIS+E+M+DPV +STG+T++R +IE+W F   K +CP T Q I + D+TPN+T
Sbjct: 12  IPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNHT 71

Query: 80  LKRLILSW---NMKESCSSSSSSSSLVEYDDVGALLKNVESS-PFKVSSLRKLRLLAAVD 139
           L+RLI SW   N         +    +   ++  L+++  SS   +V  L++LR +  V 
Sbjct: 72  LRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI--VS 131

Query: 140 DVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFSFSKRDEK- 199
           + A N  K     A   E L  IV       S D  N     EAL +L H   S+   K 
Sbjct: 132 ENATN--KRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLKN 191

Query: 200 LFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLFKSIFE 259
           L         +KS+  ++QRG  E R YA  +L+ + +       +T     ++F  + +
Sbjct: 192 LLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVVQ 251

Query: 260 LASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKKSRLRSIEAGAVCIM 319
           +  D ++++A+                    + + +L+ I     ++R +++EAG + ++
Sbjct: 252 ILDDRISQKAT-------------------KAAMHILVNICPWG-RNRHKAVEAGVISVI 311

Query: 320 IDLLPD----LNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKK-MSASNAATKI 379
           I+LL D      R   E  + +L LL +CAEGR+    H   IA+V KK +  S  A+  
Sbjct: 312 IELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDR 371

Query: 380 GVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKIMNIHGSFWR 436
            V++L  +  +     +L EM+  G V KL  +L  +  G+  TKEKA++++ +H   W+
Sbjct: 372 AVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGK--TKEKAKELLKLHARVWK 402

BLAST of Moc07g23090 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 6.6e-41
Identity = 130/453 (28.70%), Postives = 220/453 (48.57%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           +P +F CPIS+++M+DPV +STG+T++R +IE+W F+  K +CP T Q I   D+TPN+T
Sbjct: 7   IPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNHT 66

Query: 80  LKRLILSW---NMKESCSSSSSSSSLVEYDDVGALLKNVESSPF-KVSSLRKLRLLAAVD 139
           L+RLI SW   N         +    +   ++  L+K   SS   +V  L++LR +  V 
Sbjct: 67  LRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI--VS 126

Query: 140 DVAANGVKSVFSRANGFEVLVGIVNQVV-----ASESFDFANF-EACQ------------ 199
           +   N  K     A   E L  IV+  V      S S   +N  + CQ            
Sbjct: 127 ENTTN--KRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 186

Query: 200 ----EALGVLSHFSFSKRDEK-LFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQ 259
               EAL VL H   S+   K L         +K++  ++QRG  E R YA  +L+K+ +
Sbjct: 187 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 246

Query: 260 NGYDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLI 319
                 ++   +  +LF  + ++  D+++ +A+                    S +++L+
Sbjct: 247 VADPMQIILLER--ELFGEVIQILHDQISHKAT-------------------RSAMQILV 306

Query: 320 EIMESSKKSRLRSIEAGAVCIMIDLLPD----LNRSKCERTLHILKLLSECAEGRSAIAE 379
            I     ++R +++E G + ++I+LL D      R   E  + +L +L +CAEGR+    
Sbjct: 307 -ITCPWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLN 366

Query: 380 HAMGIAIVTKK-MSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNG 439
           H   IA+V+KK +  S   ++  V++L  +  +     +L+EM+  G V KL  +L  + 
Sbjct: 367 HGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSC 426

Query: 440 DGRSSTKEKARKIMNIHGSFWRRFPCFPYEFRD 441
             +  TKEKA++++ +H   WR  PC P    D
Sbjct: 427 GNK--TKEKAKELLKLHARVWRESPCVPRNLYD 431

BLAST of Moc07g23090 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.0e-29
Identity = 119/440 (27.05%), Postives = 203/440 (46.14%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTI-LNFDITPNY 79
           +P YF CPIS+E+M+DPVT  +G+T++R NI +W       +CP T Q + L+ D+TPN+
Sbjct: 10  IPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPNH 69

Query: 80  TLKRLILSWNMKESCSSSSSSSS-------LVEYDDVGALLKNVESSPFKVSSLRKLRLL 139
            L+RLI  W ++         S+       L   +++  L K  + +  +  +L+KL +L
Sbjct: 70  MLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEVL 129

Query: 140 AAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFSFSKR 199
           A          + +       + L+  V +  + +       +   E+L +L        
Sbjct: 130 A-----MDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSN 189

Query: 200 DEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLFKS 259
           D K   ++     ++S+  VL +     + Y + +L+ + +  Y  + +      ++FK 
Sbjct: 190 DAKTI-LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKG 249

Query: 260 IFELASDEMNK-------QASTLMLRDFSTIPKCRCETLFSSTL------KLLIEIMESS 319
           I     D +N           T+      ++ K     L  S +        L+ ++E+S
Sbjct: 250 IIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETS 309

Query: 320 KKSRLRS--IEAGAVCIMIDLLPDLNRSK--CERTLHILKLLSECAEGRSAIAEHAMGIA 379
             SR RS  ++ GAV  +I+L       K   E  L +L  L  CA GR+ I  H  GIA
Sbjct: 310 SWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIA 369

Query: 380 IVTKK-MSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSST 434
           +VTK+ +  S AA    + IL  +  + P   V+EEM+  G V+KL  +L    D   + 
Sbjct: 370 VVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGM--DCGLNL 429

BLAST of Moc07g23090 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.2e-28
Identity = 122/427 (28.57%), Postives = 197/427 (46.14%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           +P +FRCPIS+E+MQDPVT+ TG T++RA+IE W    N  TCP T   + +F + PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 80  LKRLILSW---NMKESCSSSSSSSSLVEYDDVGALLKNVE-------SSPFKVSSLRKLR 139
           L+RLI  W   N         +     +   V ALL           S   + ++LR+LR
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLR 133

Query: 140 LLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFSFS 199
             A   D      + + +  N  E+L+ I+     S        E   E+L +L     +
Sbjct: 134 GFARDSDKN----RVLIAAHNATEILIKILFSETTSS-------ELVSESLALLVMLPIT 193

Query: 200 KRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLF 259
           + ++    + S P  ++ +  +L   SIE R  A  +++ +       +L     +I   
Sbjct: 194 EPNQ-FVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL---KGSISNS 253

Query: 260 KSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKKSRLRSIEAG 319
           +S+FE   D +    S+          K   +TLF+         + S K +R  +I AG
Sbjct: 254 ESVFEGVLDLLRNPISS------RRALKIGIKTLFA---------LCSVKSTRHIAITAG 313

Query: 320 AVCIMID-LLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKK-MSASNAAT 379
           A  I+ID L  D +R   ER L  ++LL    EG +A  EHA+ + ++ K  +  S+ AT
Sbjct: 314 APEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRAT 373

Query: 380 KIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKIMNIHGSF 435
           +     L  +C     ER  EE  G G V +LL ++ +    R+  K+KA+K++ +    
Sbjct: 374 EYAAGALLALCTAE--ERWREEAAGAGVVVQLLLMVQSECTERA--KKKAQKLLKLLRDS 406

BLAST of Moc07g23090 vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 3.2e-27
Identity = 105/394 (26.65%), Postives = 187/394 (47.46%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           VP +F+CPIS++VM+ PV++STGVT++R +I+RW    N  TCPATMQ + N +  PN T
Sbjct: 11  VPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVPNLT 70

Query: 80  LKRLILSW--NMKESCSSSSSSSSLVEYDDVGALLKNVESSPFKVSSLRKLRLLAAVDDV 139
           L RLI  W  ++     S S  S     D++ A ++      F++ +  + ++L    + 
Sbjct: 71  LHRLIDHWSDSINRRADSESPESDTPTRDEINAAIER-----FRIENDARSKILRFARES 130

Query: 140 AANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFS---FSKRDEK 199
             N  +   +  + F  +  +V+ +  S +F  +      EA+ +LS      F +R   
Sbjct: 131 DEN--REFLAGKDDFVAM--LVDLISDSRNFSDSQLLLVGEAVKILSMIRRKIFDRRRLS 190

Query: 200 LFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLFKSIFE 259
              + +  + + S  ++++RG+ + +               D + +  + A+D    +  
Sbjct: 191 NLILTNGGDCLTSFFLLIKRGNPKLKI--------------DCSAVLEFIAVDAESKLII 250

Query: 260 LASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKKSRLRSIEAGAVCIM 319
              + +  +   L+  D  +       +L  + L LLI I  SSK+ +L  I    V  +
Sbjct: 251 AKGEGLVTEIIKLISSDSDS-------SLIEANLSLLIAI-ASSKRVKLALIREKLVTKL 310

Query: 320 IDLL--PDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKMSASNAATKIGVK 379
             LL  P  + S  E+ L +L+ +S C EGRS I +      +V K M  S AAT+  V 
Sbjct: 311 TSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMKVSTAATEHAVT 370

Query: 380 ILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNN 407
           +LW +C     ++  + ++    V K+L +L +N
Sbjct: 371 VLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

BLAST of Moc07g23090 vs. ExPASy TrEMBL
Match: A0A6J1DSJ3 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022780 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 3.8e-249
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKR 60
           MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKR
Sbjct: 1   MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKR 60

Query: 61  TCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSSSLVEYDDVGALLKNVESSPF 120
           TCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSSSLVEYDDVGALLKNVESSPF
Sbjct: 61  TCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSSSLVEYDDVGALLKNVESSPF 120

Query: 121 KVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEAL 180
           KVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEAL
Sbjct: 121 KVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEAL 180

Query: 181 GVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLL 240
           GVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLL
Sbjct: 181 GVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLL 240

Query: 241 TRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKK 300
           TRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKK
Sbjct: 241 TRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKK 300

Query: 301 SRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKM 360
           SRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKM
Sbjct: 301 SRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKM 360

Query: 361 SASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKI 420
           SASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKI
Sbjct: 361 SASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKI 420

Query: 421 MNIHGSFWRRFPCFPYEFRD 441
           MNIHGSFWRRFPCFPYEFRD
Sbjct: 421 MNIHGSFWRRFPCFPYEFRD 440

BLAST of Moc07g23090 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.2e-186
Identity = 344/446 (77.13%), Postives = 382/446 (85.65%), Query Frame = 0

Query: 1   MEEIHQNPHPPPWPPPPAAV--PPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYN 60
           ME+IHQNP PPP  P P  V  PP+FRCPISME+MQDPVTISTGV+FERANIE+WFFTYN
Sbjct: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KRTCPATMQTILNFDITPNYTLKRLILSWNMK-ESCSSSSS---SSSLVEYDDVGALLKN 120
           K+TCPATMQTI+NFDITPNYTLKRLILSW MK +SCSS+SS   SSS+  +D V  LLKN
Sbjct: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLLKN 120

Query: 121 VESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFE 180
           +ESSPFK SSL+KLRLL AVDDVAA G K VF R NGFEVL+GIV QV   ES DFANFE
Sbjct: 121 IESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANFE 180

Query: 181 ACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNG 240
           AC+EALGVLS F FSK  EK FE+LS+PE+IKSMA+VLQRGSIEGRFYAMEMLQ++ + G
Sbjct: 181 ACEEALGVLSQFPFSK-TEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEI 300
           YDWN LT++Q IDLFKSI ELASDE+ K+AS +   D S +  CR  TLFSS L+LLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIA 360
           MESSKKSRL SIEAGAVC+MIDLLPD NRSKCE+ LHILKLLSECAEGRSA+ EH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360

Query: 361 IVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTK 420
            VTKKMS SN ATKIG+KILWL+CNYHP ERVLEEMMGCG VKKLLGIL NNGDGRSSTK
Sbjct: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL-NNGDGRSSTK 420

Query: 421 EKARKIMNIHGSFWRRFPCFPYEFRD 441
           EKA+K+M +HGSFWRRFPCFPYE+RD
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRD 444

BLAST of Moc07g23090 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.7e-183
Identity = 339/447 (75.84%), Postives = 380/447 (85.01%), Query Frame = 0

Query: 1   MEEIHQNPHPP---PWPPPPAAV--PPYFRCPISMEVMQDPVTISTGVTFERANIERWFF 60
           ME+IHQ+P PP   P PPP A+V  PP+FRCPISME+MQDPVTISTGV+FERANIE+WFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKRTCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSS--SSSSLVEYDDVGALLK 120
           TYNK+TCPATMQTI+NFDITPNYTLKRLILSW MKESCSS+S   SSS+  +D V  LLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NVESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANF 180
           N+ESSPFK SSL+KLRLL AVDDVAA G K VF R NGFE+LV IV QV   E  DFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQN 240
           EAC+EALGVLS F FSK  EK FE+LS+PE+IKSMA++LQRGSIEGRFYAMEMLQ++ + 
Sbjct: 181 EACEEALGVLSQFPFSK-TEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKK 240

Query: 241 GYDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIE 300
           GYDWN LT++Q IDLFKSI ELASDE+ K+AS +   D STI +CR  TLFSS L+LLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGI 360
           IMESSKKSRL SIEAGAVC+MIDLLPD NRSKCE+ L+ILKLLSECAEGRSA+ EH MGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGI 360

Query: 361 AIVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSST 420
           A VTKKMS SN ATKIG+KILW +C+YHP ERVLEEMMGCG VKKLLGIL  NGDG SST
Sbjct: 361 ATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGIL--NGDGSSST 420

Query: 421 KEKARKIMNIHGSFWRRFPCFPYEFRD 441
           KEKA K+M +HGSFWRRFPCFPYE+RD
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRD 444

BLAST of Moc07g23090 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.9e-180
Identity = 335/448 (74.78%), Postives = 378/448 (84.38%), Query Frame = 0

Query: 1   MEEIHQN--PHPPPWPPPPAA--VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFT 60
           ME+I QN  P P P PPPP    +PPYFRCPISMEVM DPVTISTGV+FERANIE+WFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKRTCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSS---SLVEYDDVGALLK 120
           YNK+TCPATMQ I+NFDITPNYTLKRLILSW M ESCSS+SSSS     +EYDDV ALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NVESSPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANF 180
           N+E SPFK SSL+KLRLL A+DDV A G K +F +ANGFEVLVG+V Q+   ES +FANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACQEALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQN 240
           EAC+EALGVLS F FSK  EKLFE+L++PE+IKSMA+VLQRGSIEGRFYAME+LQ++ + 
Sbjct: 181 EACEEALGVLSQFPFSK-IEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIE 300
           GYD+N L+++ AIDLFKSI ELASDE+ K+AST+ + D S    CR  TLFSS L+LLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGI 360
           IMESSKK RL  IEAGAVCIMIDLLPD NRSKCE+ LH+LKLLS+CAEGR A+AEH MGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 AIVTKKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGR-SS 420
           A VTKKMS SN ATKIGVKILWL+CNYHP ERVLEEM+GCG VKKL+GIL  NGDGR SS
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGIL--NGDGRSSS 420

Query: 421 TKEKARKIMNIHGSFWRRFPCFPYEFRD 441
           TKEKA KIM +HGSFWRRFPCFPYEFRD
Sbjct: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRD 445

BLAST of Moc07g23090 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 6.4e-180
Identity = 334/444 (75.23%), Postives = 374/444 (84.23%), Query Frame = 0

Query: 1   MEEIHQNPHPPPWPPPPAAVPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKR 60
           ME+I QN  PPP PP    +PPYFRCPISMEVM DPVTISTGV+FERANIE+WFFTYNK+
Sbjct: 1   MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKK 60

Query: 61  TCPATMQTILNFDITPNYTLKRLILSWNMKESCSSSSSSS---SLVEYDDVGALLKNVES 120
           TCPATMQ I+NFDITPNYTLKRLILSW M ESCS +SSSS     VEYDDV ALLKN+E 
Sbjct: 61  TCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIEL 120

Query: 121 SPFKVSSLRKLRLLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQ 180
           SPFK SSL+KLRLL A+DDVAA   K++F +ANGFEVLVG+V QV   ES +FANFEAC+
Sbjct: 121 SPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACE 180

Query: 181 EALGVLSHFSFSKRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDW 240
           EALGVLS F FSK  EKLFE+LSK E+IKSM +VLQRGSIEGRFYAME+LQ++ + GYD+
Sbjct: 181 EALGVLSQFPFSK-TEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDY 240

Query: 241 NLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMES 300
           N L+++ AIDLFKSI ELASDE+ K+AST+   D S   +CR  TLFSS L+LLIEIMES
Sbjct: 241 NSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMES 300

Query: 301 SKKSRLRSIEAGAVCIMIDLLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVT 360
           SKK RL  IEAGA+CIMIDLLPD NRSKCE+ LH+LKLLS+CAEGR A+AEH MGIA VT
Sbjct: 301 SKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVT 360

Query: 361 KKMSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGR-SSTKEK 420
           KKMS SN ATKIGVKILWL+CNYHP ERVLEEMMGCG VKKL+GIL  NGDGR SSTKEK
Sbjct: 361 KKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGIL--NGDGRSSSTKEK 420

Query: 421 ARKIMNIHGSFWRRFPCFPYEFRD 441
           A KIM +HGSFWRRFPCFPYEFRD
Sbjct: 421 AMKIMKLHGSFWRRFPCFPYEFRD 441

BLAST of Moc07g23090 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 176.4 bits (446), Expect = 5.0e-44
Identity = 129/426 (30.28%), Postives = 213/426 (50.00%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           +PP+F CPIS+E+M+DPV +STG+T++R +IE+W F   K +CP T Q I + D+TPN+T
Sbjct: 12  IPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNHT 71

Query: 80  LKRLILSW---NMKESCSSSSSSSSLVEYDDVGALLKNVESS-PFKVSSLRKLRLLAAVD 139
           L+RLI SW   N         +    +   ++  L+++  SS   +V  L++LR +  V 
Sbjct: 72  LRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI--VS 131

Query: 140 DVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFSFSKRDEK- 199
           + A N  K     A   E L  IV       S D  N     EAL +L H   S+   K 
Sbjct: 132 ENATN--KRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLKN 191

Query: 200 LFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLFKSIFE 259
           L         +KS+  ++QRG  E R YA  +L+ + +       +T     ++F  + +
Sbjct: 192 LLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVVQ 251

Query: 260 LASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKKSRLRSIEAGAVCIM 319
           +  D ++++A+                    + + +L+ I     ++R +++EAG + ++
Sbjct: 252 ILDDRISQKAT-------------------KAAMHILVNICPWG-RNRHKAVEAGVISVI 311

Query: 320 IDLLPD----LNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKK-MSASNAATKI 379
           I+LL D      R   E  + +L LL +CAEGR+    H   IA+V KK +  S  A+  
Sbjct: 312 IELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDR 371

Query: 380 GVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKIMNIHGSFWR 436
            V++L  +  +     +L EM+  G V KL  +L  +  G+  TKEKA++++ +H   W+
Sbjct: 372 AVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGK--TKEKAKELLKLHARVWK 402

BLAST of Moc07g23090 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 169.9 bits (429), Expect = 4.7e-42
Identity = 130/453 (28.70%), Postives = 220/453 (48.57%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           +P +F CPIS+++M+DPV +STG+T++R +IE+W F+  K +CP T Q I   D+TPN+T
Sbjct: 7   IPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNHT 66

Query: 80  LKRLILSW---NMKESCSSSSSSSSLVEYDDVGALLKNVESSPF-KVSSLRKLRLLAAVD 139
           L+RLI SW   N         +    +   ++  L+K   SS   +V  L++LR +  V 
Sbjct: 67  LRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI--VS 126

Query: 140 DVAANGVKSVFSRANGFEVLVGIVNQVV-----ASESFDFANF-EACQ------------ 199
           +   N  K     A   E L  IV+  V      S S   +N  + CQ            
Sbjct: 127 ENTTN--KRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 186

Query: 200 ----EALGVLSHFSFSKRDEK-LFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQ 259
               EAL VL H   S+   K L         +K++  ++QRG  E R YA  +L+K+ +
Sbjct: 187 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 246

Query: 260 NGYDWNLLTRYQAIDLFKSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLI 319
                 ++   +  +LF  + ++  D+++ +A+                    S +++L+
Sbjct: 247 VADPMQIILLER--ELFGEVIQILHDQISHKAT-------------------RSAMQILV 306

Query: 320 EIMESSKKSRLRSIEAGAVCIMIDLLPD----LNRSKCERTLHILKLLSECAEGRSAIAE 379
            I     ++R +++E G + ++I+LL D      R   E  + +L +L +CAEGR+    
Sbjct: 307 -ITCPWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLN 366

Query: 380 HAMGIAIVTKK-MSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNG 439
           H   IA+V+KK +  S   ++  V++L  +  +     +L+EM+  G V KL  +L  + 
Sbjct: 367 HGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSC 426

Query: 440 DGRSSTKEKARKIMNIHGSFWRRFPCFPYEFRD 441
             +  TKEKA++++ +H   WR  PC P    D
Sbjct: 427 GNK--TKEKAKELLKLHARVWRESPCVPRNLYD 431

BLAST of Moc07g23090 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 131.7 bits (330), Expect = 1.4e-30
Identity = 119/440 (27.05%), Postives = 203/440 (46.14%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTI-LNFDITPNY 79
           +P YF CPIS+E+M+DPVT  +G+T++R NI +W       +CP T Q + L+ D+TPN+
Sbjct: 24  IPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPNH 83

Query: 80  TLKRLILSWNMKESCSSSSSSSS-------LVEYDDVGALLKNVESSPFKVSSLRKLRLL 139
            L+RLI  W ++         S+       L   +++  L K  + +  +  +L+KL +L
Sbjct: 84  MLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEVL 143

Query: 140 AAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFSFSKR 199
           A          + +       + L+  V +  + +       +   E+L +L        
Sbjct: 144 A-----MDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSN 203

Query: 200 DEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLFKS 259
           D K   ++     ++S+  VL +     + Y + +L+ + +  Y  + +      ++FK 
Sbjct: 204 DAKTI-LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKG 263

Query: 260 IFELASDEMNK-------QASTLMLRDFSTIPKCRCETLFSSTL------KLLIEIMESS 319
           I     D +N           T+      ++ K     L  S +        L+ ++E+S
Sbjct: 264 IIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETS 323

Query: 320 KKSRLRS--IEAGAVCIMIDLLPDLNRSK--CERTLHILKLLSECAEGRSAIAEHAMGIA 379
             SR RS  ++ GAV  +I+L       K   E  L +L  L  CA GR+ I  H  GIA
Sbjct: 324 SWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIA 383

Query: 380 IVTKK-MSASNAATKIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSST 434
           +VTK+ +  S AA    + IL  +  + P   V+EEM+  G V+KL  +L    D   + 
Sbjct: 384 VVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGM--DCGLNL 443

BLAST of Moc07g23090 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-29
Identity = 122/427 (28.57%), Postives = 197/427 (46.14%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           +P +FRCPIS+E+MQDPVT+ TG T++RA+IE W    N  TCP T   + +F + PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 80  LKRLILSW---NMKESCSSSSSSSSLVEYDDVGALLKNVE-------SSPFKVSSLRKLR 139
           L+RLI  W   N         +     +   V ALL           S   + ++LR+LR
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLR 133

Query: 140 LLAAVDDVAANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFSFS 199
             A   D      + + +  N  E+L+ I+     S        E   E+L +L     +
Sbjct: 134 GFARDSDKN----RVLIAAHNATEILIKILFSETTSS-------ELVSESLALLVMLPIT 193

Query: 200 KRDEKLFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLF 259
           + ++    + S P  ++ +  +L   SIE R  A  +++ +       +L     +I   
Sbjct: 194 EPNQ-FVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL---KGSISNS 253

Query: 260 KSIFELASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKKSRLRSIEAG 319
           +S+FE   D +    S+          K   +TLF+         + S K +R  +I AG
Sbjct: 254 ESVFEGVLDLLRNPISS------RRALKIGIKTLFA---------LCSVKSTRHIAITAG 313

Query: 320 AVCIMID-LLPDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKK-MSASNAAT 379
           A  I+ID L  D +R   ER L  ++LL    EG +A  EHA+ + ++ K  +  S+ AT
Sbjct: 314 APEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRAT 373

Query: 380 KIGVKILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNNGDGRSSTKEKARKIMNIHGSF 435
           +     L  +C     ER  EE  G G V +LL ++ +    R+  K+KA+K++ +    
Sbjct: 374 EYAAGALLALCTAE--ERWREEAAGAGVVVQLLLMVQSECTERA--KKKAQKLLKLLRDS 406

BLAST of Moc07g23090 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 2.3e-28
Identity = 105/394 (26.65%), Postives = 187/394 (47.46%), Query Frame = 0

Query: 20  VPPYFRCPISMEVMQDPVTISTGVTFERANIERWFFTYNKRTCPATMQTILNFDITPNYT 79
           VP +F+CPIS++VM+ PV++STGVT++R +I+RW    N  TCPATMQ + N +  PN T
Sbjct: 11  VPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVPNLT 70

Query: 80  LKRLILSW--NMKESCSSSSSSSSLVEYDDVGALLKNVESSPFKVSSLRKLRLLAAVDDV 139
           L RLI  W  ++     S S  S     D++ A ++      F++ +  + ++L    + 
Sbjct: 71  LHRLIDHWSDSINRRADSESPESDTPTRDEINAAIER-----FRIENDARSKILRFARES 130

Query: 140 AANGVKSVFSRANGFEVLVGIVNQVVASESFDFANFEACQEALGVLSHFS---FSKRDEK 199
             N  +   +  + F  +  +V+ +  S +F  +      EA+ +LS      F +R   
Sbjct: 131 DEN--REFLAGKDDFVAM--LVDLISDSRNFSDSQLLLVGEAVKILSMIRRKIFDRRRLS 190

Query: 200 LFEMLSKPESIKSMAVVLQRGSIEGRFYAMEMLQKMCQNGYDWNLLTRYQAIDLFKSIFE 259
              + +  + + S  ++++RG+ + +               D + +  + A+D    +  
Sbjct: 191 NLILTNGGDCLTSFFLLIKRGNPKLKI--------------DCSAVLEFIAVDAESKLII 250

Query: 260 LASDEMNKQASTLMLRDFSTIPKCRCETLFSSTLKLLIEIMESSKKSRLRSIEAGAVCIM 319
              + +  +   L+  D  +       +L  + L LLI I  SSK+ +L  I    V  +
Sbjct: 251 AKGEGLVTEIIKLISSDSDS-------SLIEANLSLLIAI-ASSKRVKLALIREKLVTKL 310

Query: 320 IDLL--PDLNRSKCERTLHILKLLSECAEGRSAIAEHAMGIAIVTKKMSASNAATKIGVK 379
             LL  P  + S  E+ L +L+ +S C EGRS I +      +V K M  S AAT+  V 
Sbjct: 311 TSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMKVSTAATEHAVT 370

Query: 380 ILWLMCNYHPGERVLEEMMGCGAVKKLLGILNNN 407
           +LW +C     ++  + ++    V K+L +L +N
Sbjct: 371 VLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155721.17.8e-249100.00E3 ubiquitin-protein ligase PUB23-like [Momordica charantia][more]
XP_004145072.22.5e-18677.13E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical pro... [more]
XP_038875803.13.2e-18677.58E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
XP_008460073.19.7e-18375.84PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo][more]
XP_023549278.11.7e-18276.12E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q84TG37.1e-4330.28E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC66.6e-4128.70E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF152.0e-2927.05E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT792.2e-2828.57U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9LXE33.2e-2726.65U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1DSJ33.8e-249100.00RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102278... [more]
A0A0A0KBP61.2e-18677.13RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE... [more]
A0A1S3CC544.7e-18375.84RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ... [more]
A0A6J1H6I82.9e-18074.78RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075... [more]
A0A6J1KWQ86.4e-18075.23RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P... [more]
Match NameE-valueIdentityDescription
AT2G35930.15.0e-4430.28plant U-box 23 [more]
AT3G52450.14.7e-4228.70plant U-box 22 [more]
AT3G11840.11.4e-3027.05plant U-box 24 [more]
AT3G19380.11.6e-2928.57plant U-box 25 [more]
AT5G09800.12.3e-2826.65ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 23..87
e-value: 1.7E-24
score: 97.4
IPR003613U box domainPFAMPF04564U-boxcoord: 20..91
e-value: 9.6E-17
score: 60.9
IPR003613U box domainPROSITEPS51698U_BOXcoord: 19..94
score: 29.157194
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 19..90
e-value: 8.5E-23
score: 82.1
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 91..422
e-value: 1.9E-10
score: 41.9
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 20..440
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 20..440
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 24..67
e-value: 7.67801E-22
score: 86.1334
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 15..91
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 146..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc07g23090.1Moc07g23090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity