Moc07g21280 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g21280
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 ubiquitin-protein like
Location	chr7: 15578928 .. 15579419 (+)
RNA-Seq Expression	Moc07g21280
Synteny	Moc07g21280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTTCCGGTGGGTTGCACGGAGGTATTCTTCCCGACAATTTTCCTCCACTTCCTCTCCCTCCTCGATTTCCTCAAAAAGCTCATTCTGACGCTCTTCCGCCTGCTGGGCCTGCCGGAGTTTATCGAGACGGACGGAATCTGGCCGGAGCCCCGATTGCCCGCCAACCCGCCCGCGTCGGCCGTCCTGATCCGCGAATTCCTGCCGGTCGTCAAATTCTCCGACGTCGCCGAGGCCTCCGGCGGCGAGAGCTGCGCCGTTTGCCTGTACGAGTTCGAGGCGGCGGAGGAAATCCGGTGGCTGACGAATTGCCGGCATATTTTTCACCGGGGGTGTCTGGACCGTTGGATGGATCACGATCAGAAGACGTGTCCGCTGTGCAGGACGCCGTTTGTCCCAGATGGGATGCAGGACGAGTTCAATCAACGGCTCTGGGCCGCCTCCGGAATCGCTGAGTTCGATCCCGAGTTCAACTCGGTTTTAGGTTAG

mRNA sequence

ATGGGTTTTCCGGTGGGTTGCACGGAGGTATTCTTCCCGACAATTTTCCTCCACTTCCTCTCCCTCCTCGATTTCCTCAAAAAGCTCATTCTGACGCTCTTCCGCCTGCTGGGCCTGCCGGAGTTTATCGAGACGGACGGAATCTGGCCGGAGCCCCGATTGCCCGCCAACCCGCCCGCGTCGGCCGTCCTGATCCGCGAATTCCTGCCGGTCGTCAAATTCTCCGACGTCGCCGAGGCCTCCGGCGGCGAGAGCTGCGCCGTTTGCCTGTACGAGTTCGAGGCGGCGGAGGAAATCCGGTGGCTGACGAATTGCCGGCATATTTTTCACCGGGGGTGTCTGGACCGTTGGATGGATCACGATCAGAAGACGTGTCCGCTGTGCAGGACGCCGTTTGTCCCAGATGGGATGCAGGACGAGTTCAATCAACGGCTCTGGGCCGCCTCCGGAATCGCTGAGTTCGATCCCGAGTTCAACTCGGTTTTAGGTTAG

Coding sequence (CDS)

ATGGGTTTTCCGGTGGGTTGCACGGAGGTATTCTTCCCGACAATTTTCCTCCACTTCCTCTCCCTCCTCGATTTCCTCAAAAAGCTCATTCTGACGCTCTTCCGCCTGCTGGGCCTGCCGGAGTTTATCGAGACGGACGGAATCTGGCCGGAGCCCCGATTGCCCGCCAACCCGCCCGCGTCGGCCGTCCTGATCCGCGAATTCCTGCCGGTCGTCAAATTCTCCGACGTCGCCGAGGCCTCCGGCGGCGAGAGCTGCGCCGTTTGCCTGTACGAGTTCGAGGCGGCGGAGGAAATCCGGTGGCTGACGAATTGCCGGCATATTTTTCACCGGGGGTGTCTGGACCGTTGGATGGATCACGATCAGAAGACGTGTCCGCTGTGCAGGACGCCGTTTGTCCCAGATGGGATGCAGGACGAGTTCAATCAACGGCTCTGGGCCGCCTCCGGAATCGCTGAGTTCGATCCCGAGTTCAACTCGGTTTTAGGTTAG

Protein sequence

MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPPASAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMDHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNSVLG

Homology

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BLAST of Moc07g21280 vs. NCBI nr
Match: XP_023000136.1 (probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima])

HSP 1 Score: 289.7 bits (740), Expect = 1.6e-74
Identity = 138/164 (84.15%), Postives = 147/164 (89.63%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETD-GIWPEPRLPANPP 60
           MGFPVGCTEVFFPTIFLH LSLLD LK+LIL LFRLLGLPEF+ +D  + PE   P NPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  ASAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMD 120
           ASAVL+REFLPVVKFSDV +A+  ESCAVCLYEFE AEEIRWLTNC+HIFHRGC+DRWMD
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAA-AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMD 120

Query: 121 HDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNSVLG 164
           HDQKTCPLCRTPFVPDGM DEFNQRLWAASGIAEFD EFNSVLG
Sbjct: 121 HDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVLG 163

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BLAST of Moc07g21280 vs. NCBI nr
Match: KAG6593657.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025989.1 putative E3 ubiquitin-protein ligase RHA1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 285.4 bits (729), Expect = 3.0e-73
Identity = 136/166 (81.93%), Postives = 147/166 (88.55%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETD-GIWPEPRLPANPP 60
           MGFPVGCTEVFFPTIFLH LSLLD LK+LIL LFRLLGLPEF+ +D  +  E  +P NPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSREVGIPLNPP 60

Query: 61  ASAVLIREFLPVVKFSDVAEA--SGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW 120
           ASAV++REFLPVVKFSDV +A  +  ESCAVCLYEFE AEEIRWLTNC+HIFHRGC+DRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNSVLG 164
           MDHDQKTCPLCRTPFVPDGM DEFNQRLWAASGIAEFD EFNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVLG 166

[Close]

BLAST of Moc07g21280 vs. NCBI nr
Match: XP_022964361.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata])

HSP 1 Score: 285.0 bits (728), Expect = 4.0e-73
Identity = 137/166 (82.53%), Postives = 147/166 (88.55%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETD-GIWPEPRLPANPP 60
           MGFPVGCTEVFFPTIFLH LSLLD LK+LIL LFRLLGLPEF+ +D  I  E  +P NPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLYSDAAISREGGIPLNPP 60

Query: 61  ASAVLIREFLPVVKFSDVAEA--SGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW 120
           ASAV++REFLPVVKFSDV +A  +  ESCAVCLYEFE AEEIRWLTNC+HIFHRGC+DRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNSVLG 164
           MDHDQKTCPLCRTPFVPDGM DEFNQRLWAASGIAEFD EFNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVLG 166

[Close]

BLAST of Moc07g21280 vs. NCBI nr
Match: XP_023514786.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 283.5 bits (724), Expect = 1.2e-72
Identity = 136/166 (81.93%), Postives = 146/166 (87.95%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETD-GIWPEPRLPANPP 60
           MGFPVGCTEVFFPTIFLH LSLLD LK+LIL LFRLLGLPEF+ +D  I  E  +P NPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDSAISREVGIPLNPP 60

Query: 61  ASAVLIREFLPVVKFSDVAEA--SGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW 120
           ASAV++REFLPVVKFSDV +A  +  ESCAVCLYEFE AEEIRWLTNC+HIFHRGC+DRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNSVLG 164
           MDHDQKTCPLCRTPFVPDGM DEFNQRLWAASGIAE D EFNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAELDSEFNSVLG 166

[Close]

BLAST of Moc07g21280 vs. NCBI nr
Match: XP_008458180.1 (PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo] >TYK03034.1 E3 ubiquitin-protein ligase RHA1B [Cucumis melo var. makuwa])

HSP 1 Score: 266.9 bits (681), Expect = 1.1e-67
Identity = 127/164 (77.44%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPPA 60
           MGFPVGCTEVFFPTIFLH LSLLD LK LIL+LFRLLGLPEF+ +D + PE + P NPP 
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMDH 120
           SA+L+RE LPVVKFSD+      E CAVCLYEFE AEEIRWLTNC+HIFHR CLDRWMDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPP--ERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDH 120

Query: 121 DQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAE-FDPEFNSVLG 164
           D  TCPLCRTPFVPDGM DEFNQRLWAASG+AE F+ +FNSVLG
Sbjct: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

[Close]

BLAST of Moc07g21280 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.7e-55
Identity = 100/162 (61.73%), Postives = 123/162 (75.93%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPE-----PRLP 60
           MGFPVG TEVF P +F+  LS+L F++ ++ ++FR LGL +F+E D  WP+      R+P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  -ANPPASAVLIREFLPVVKFSDVAEASGG--ESCAVCLYEFEAAEEIRWLTNCRHIFHRG 120
               P SA+LIRE LPV+KF ++  +     E+CAVCLYEFE  +EIRWL NCRHIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CLDRWMDHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEF 155
           CLDRWMDHDQKTCPLCRTPFVPD MQ+EFNQRLWAASG+ +F
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGVHDF 162

[Close]

BLAST of Moc07g21280 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-18
Identity = 55/162 (33.95%), Postives = 86/162 (53.09%), Query Frame = 0


Query: 1   MGFPVG-CTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPP 60
           MG P    TE+  P+  L  L ++ F + ++  L   +GLP F++ +             
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  ASAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW-M 120
            SA L  E +PVV+FSD+      + C VCL +FE+ +++R L  C H+FH  CLDRW +
Sbjct: 61  TSASLANELIPVVRFSDL-PTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWIV 120

Query: 121 DHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNS 161
           D+++  CP+CR  F+P   ++++ Q  W  SG   F  E  S
Sbjct: 121 DYNKMKCPVCRHRFLP---KEKYTQSDW-GSGSDWFSDEVES 157

[Close]

BLAST of Moc07g21280 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 51/150 (34.00%), Postives = 77/150 (51.33%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPPA 60
           MG P    E+  P   L  L ++ F + ++  L   +GLP F++ +              
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW-MD 120
           SA L  E +PVV+FSD+      + C VCL +F + ++IR L  C H+FH  CLDRW +D
Sbjct: 61  SATLANELIPVVRFSDLL-TDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVD 120

Query: 121 HDQKTCPLCRTPFVPDGMQDEFNQRLWAAS 150
            ++ TCP+CR  F+P+     F+   W  S
Sbjct: 121 CNKITCPICRNRFLPEEKSTPFD---WGTS 146

[Close]

BLAST of Moc07g21280 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 1.5e-11
Identity = 30/63 (47.62%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 67  EFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMDHDQKTCP 126
           E LP+ +FS +  +  G  C+VCL +FE+ E +R L  CRH FH GC+D+W++    TCP
Sbjct: 104 ESLPLFRFSALKGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLE-QHATCP 163

Query: 127 LCR 130
           LCR
Sbjct: 164 LCR 165

[Close]

BLAST of Moc07g21280 vs. ExPASy Swiss-Prot
Match: Q9SK92 (E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-11
Identity = 37/91 (40.66%), Postives = 45/91 (49.45%), Query Frame = 0


Query: 47  GIWPEPRLPANPPASAVLIREFLPVVKFSDVAEASGGE---SCAVCLYEFEAAEEIRWLT 106
           G W   R    P   A +I  F P   +S V     G+    C VCL EFE  E +R + 
Sbjct: 77  GNWLNVRQTTEPGLDASVIETF-PTFPYSTVKTLRIGKEALECPVCLNEFEDDETLRLIP 136

Query: 107 NCRHIFHRGCLDRWMDHDQKTCPLCRTPFVP 135
            C H+FH GC+D W+   Q TCPLCR   VP
Sbjct: 137 QCCHVFHPGCIDAWL-RSQTTCPLCRANLVP 165

[Close]

BLAST of Moc07g21280 vs. ExPASy TrEMBL
Match: A0A6J1KF16 (probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111494424 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 7.8e-75
Identity = 138/164 (84.15%), Postives = 147/164 (89.63%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETD-GIWPEPRLPANPP 60
           MGFPVGCTEVFFPTIFLH LSLLD LK+LIL LFRLLGLPEF+ +D  + PE   P NPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  ASAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMD 120
           ASAVL+REFLPVVKFSDV +A+  ESCAVCLYEFE AEEIRWLTNC+HIFHRGC+DRWMD
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAA-AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMD 120

Query: 121 HDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNSVLG 164
           HDQKTCPLCRTPFVPDGM DEFNQRLWAASGIAEFD EFNSVLG
Sbjct: 121 HDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVLG 163

[Close]

BLAST of Moc07g21280 vs. ExPASy TrEMBL
Match: A0A6J1HIP9 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111464396 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.9e-73
Identity = 137/166 (82.53%), Postives = 147/166 (88.55%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETD-GIWPEPRLPANPP 60
           MGFPVGCTEVFFPTIFLH LSLLD LK+LIL LFRLLGLPEF+ +D  I  E  +P NPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLYSDAAISREGGIPLNPP 60

Query: 61  ASAVLIREFLPVVKFSDVAEA--SGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW 120
           ASAV++REFLPVVKFSDV +A  +  ESCAVCLYEFE AEEIRWLTNC+HIFHRGC+DRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNSVLG 164
           MDHDQKTCPLCRTPFVPDGM DEFNQRLWAASGIAEFD EFNSVLG
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVLG 166

[Close]

BLAST of Moc07g21280 vs. ExPASy TrEMBL
Match: A0A5D3BV35 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001290 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.4e-68
Identity = 127/164 (77.44%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPPA 60
           MGFPVGCTEVFFPTIFLH LSLLD LK LIL+LFRLLGLPEF+ +D + PE + P NPP 
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMDH 120
           SA+L+RE LPVVKFSD+      E CAVCLYEFE AEEIRWLTNC+HIFHR CLDRWMDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPP--ERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDH 120

Query: 121 DQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAE-FDPEFNSVLG 164
           D  TCPLCRTPFVPDGM DEFNQRLWAASG+AE F+ +FNSVLG
Sbjct: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

[Close]

BLAST of Moc07g21280 vs. ExPASy TrEMBL
Match: A0A1S3C782 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.4e-68
Identity = 127/164 (77.44%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPPA 60
           MGFPVGCTEVFFPTIFLH LSLLD LK LIL+LFRLLGLPEF+ +D + PE + P NPP 
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMDH 120
           SA+L+RE LPVVKFSD+      E CAVCLYEFE AEEIRWLTNC+HIFHR CLDRWMDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPP--ERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDH 120

Query: 121 DQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAE-FDPEFNSVLG 164
           D  TCPLCRTPFVPDGM DEFNQRLWAASG+AE F+ +FNSVLG
Sbjct: 121 DHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

[Close]

BLAST of Moc07g21280 vs. ExPASy TrEMBL
Match: A0A0A0K874 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 5.1e-66
Identity = 125/164 (76.22%), Postives = 137/164 (83.54%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPPA 60
           MGFPVGCTEVFFPTIFLH LSLLD LK LIL+LFRLLGLPEF+ +D + PE + P NPP 
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDVLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRWMDH 120
           SA+L+RE LPVVKFSD+      E CAVCLYEFE AEEIRWLTNC+HIFHR C+D WMDH
Sbjct: 61  SALLLRELLPVVKFSDLPNPP--ERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDSWMDH 120

Query: 121 DQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAE-FDPEFNSVLG 164
           D  TCPLCRTPFVPD M DEFNQRLWAASGIAE F+ +FNSVLG
Sbjct: 121 DHDTCPLCRTPFVPDEMMDEFNQRLWAASGIAETFETDFNSVLG 162

[Close]

BLAST of Moc07g21280 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 216.9 bits (551), Expect = 1.2e-56
Identity = 100/162 (61.73%), Postives = 123/162 (75.93%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPE-----PRLP 60
           MGFPVG TEVF P +F+  LS+L F++ ++ ++FR LGL +F+E D  WP+      R+P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  -ANPPASAVLIREFLPVVKFSDVAEASGG--ESCAVCLYEFEAAEEIRWLTNCRHIFHRG 120
               P SA+LIRE LPV+KF ++  +     E+CAVCLYEFE  +EIRWL NCRHIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CLDRWMDHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEF 155
           CLDRWMDHDQKTCPLCRTPFVPD MQ+EFNQRLWAASG+ +F
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGVHDF 162

[Close]

BLAST of Moc07g21280 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 4.9e-45
Identity = 86/154 (55.84%), Postives = 112/154 (72.73%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGI---WPE-PRLPA 60
           MGFPVG +E+  P IF + LS L  ++KLI T+F+++GLP+F+E + +   WP+ P    
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPEPVSTSWPDPPPTLT 60

Query: 61  NPPASAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDR 120
            P ++A+L  E LPVV+FSD+      E CAVCLY+FE  +EIR LTNCRHIFHRGCLDR
Sbjct: 61  KPDSAAILAGEMLPVVRFSDINRPE-SECCAVCLYDFENDDEIRRLTNCRHIFHRGCLDR 120

Query: 121 W-MDHDQKTCPLCRTPFVPDGMQDEFNQRLWAAS 150
           W M ++Q TCPLCRT F+PD +Q EFNQRLW+ S
Sbjct: 121 WMMGYNQMTCPLCRTQFIPDHLQLEFNQRLWSQS 153

[Close]

BLAST of Moc07g21280 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 1.9e-41
Identity = 86/169 (50.89%), Postives = 111/169 (65.68%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGI-----WPEPRLP 60
           MG+PVG TE+  P IFLH LSLL  ++ LI T FR+LGLP+F+E+D +     W EP   
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  ANP-----------PASAVLIREFLPVVKFSDVAE---ASGGESCAVCLYEFEAAEEIRW 120
           +             P +A L  E LPV++FS++      SG + CAVCL+EFE  +EIR 
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LTNCRHIFHRGCLDRW-MDHDQKTCPLCRTPFVPDGMQDEFNQRLWAAS 150
           LTNC+HIFHR CLDRW M ++Q TCPLCRTPF+ D +Q  FNQR+W+ S
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVAFNQRVWSES 169

[Close]

BLAST of Moc07g21280 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 92.8 bits (229), Expect = 2.7e-19
Identity = 55/162 (33.95%), Postives = 86/162 (53.09%), Query Frame = 0


Query: 1   MGFPVG-CTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPP 60
           MG P    TE+  P+  L  L ++ F + ++  L   +GLP F++ +             
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  ASAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW-M 120
            SA L  E +PVV+FSD+      + C VCL +FE+ +++R L  C H+FH  CLDRW +
Sbjct: 61  TSASLANELIPVVRFSDL-PTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWIV 120

Query: 121 DHDQKTCPLCRTPFVPDGMQDEFNQRLWAASGIAEFDPEFNS 161
           D+++  CP+CR  F+P   ++++ Q  W  SG   F  E  S
Sbjct: 121 DYNKMKCPVCRHRFLP---KEKYTQSDW-GSGSDWFSDEVES 157

[Close]

BLAST of Moc07g21280 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 91.3 bits (225), Expect = 7.9e-19
Identity = 51/150 (34.00%), Postives = 77/150 (51.33%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDFLKKLILTLFRLLGLPEFIETDGIWPEPRLPANPPA 60
           MG P    E+  P   L  L ++ F + ++  L   +GLP F++ +              
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SAVLIREFLPVVKFSDVAEASGGESCAVCLYEFEAAEEIRWLTNCRHIFHRGCLDRW-MD 120
           SA L  E +PVV+FSD+      + C VCL +F + ++IR L  C H+FH  CLDRW +D
Sbjct: 61  SATLANELIPVVRFSDLL-TDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVD 120

Query: 121 HDQKTCPLCRTPFVPDGMQDEFNQRLWAAS 150
            ++ TCP+CR  F+P+     F+   W  S
Sbjct: 121 CNKITCPICRNRFLPEEKSTPFD---WGTS 146

The following BLAST results are available for this feature:

BLAST of Moc07g21280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000136.1	1.6e-74	84.15	probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima]	[more]
KAG6593657.1	3.0e-73	81.93	Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022964361.1	4.0e-73	82.53	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata]	[more]
XP_023514786.1	1.2e-72	81.93	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo]	[more]
XP_008458180.1	1.1e-67	77.44	PREDICTED: E3 ubiquitin-protein ligase RHA1B [Cucumis melo] >TYK03034.1 E3 ubiqu...	[more]

BLAST of Moc07g21280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	1.7e-55	61.73	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS4	3.8e-18	33.95	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SUS5	1.1e-17	34.00	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q5XF85	1.5e-11	47.62	E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 ...	[more]
Q9SK92	3.4e-11	40.66	E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 ...	[more]

BLAST of Moc07g21280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KF16	7.8e-75	84.15	probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1HIP9	1.9e-73	82.53	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A5D3BV35	5.4e-68	77.44	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3C782	5.4e-68	77.44	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 S...	[more]
A0A0A0K874	5.1e-66	76.22	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 P...	[more]

BLAST of Moc07g21280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61460.1	1.2e-56	61.73	brassinosteroid-responsive RING-H2	[more]
AT1G63840.1	4.9e-45	55.84	RING/U-box superfamily protein	[more]
AT5G41400.1	1.9e-41	50.89	RING/U-box superfamily protein	[more]
AT4G11370.1	2.7e-19	33.95	RING-H2 finger A1A	[more]
AT4G11360.1	7.9e-19	34.00	RING-H2 finger A1B	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 86..128 e-value: 2.4E-6 score: 37.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 85..129 e-value: 1.3E-12 score: 47.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 86..129 score: 12.474524
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 85..129 e-value: 0.0041 score: 10.5
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 65..135 e-value: 9.0E-20 score: 71.7
None	No IPR available	PANTHER	PTHR45969:SF10	BRH1 RING FINGER PROTEIN	coord: 1..155
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..155
None	No IPR available	CDD	cd16448	RING-H2	coord: 86..129 e-value: 5.06545E-14 score: 60.9287
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 85..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g21280.1	Moc07g21280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:1905421	regulation of plant organ morphogenesis
biological_process	GO:0009741	response to brassinosteroid
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding