Moc07g20320 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc07g20320
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon RE1
Locationchr7: 14615912 .. 14617122 (+)
RNA-Seq ExpressionMoc07g20320
SyntenyMoc07g20320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCGAAGGATTAGATACAACTTATAAGGTATGGAAATCTTTGGAAGAGACTCCCCTAGCCATTACCAAAGAAAATGAAATTCATATCAGTGAAGTTCTACACTATTTGAGGAAGGGGAATCTATCACTTGATGAATACATCAAGAAATTCAAATCCTTGAGTGACAAGCTTGCTGCCATGAAGAAACCATTGGATGATTTAAGCAAGGTATTCACTCTAGCTCGTGGCTTGGGCAATCAATATAAAGAATTTCGGATTGCTATGTTGTCAAAGACTCCATTTCCAACATTCAACCAATTTATCTTAGCACTAAAAGCTCATAGCAACTACATGTCCATGAAATAAAAGAAGAAAAGATCTCTCCTCCTGAAGCAAATCAAGCCTATTACAGTCAAAAGGGAAGAGGCAGAAGTAGAGGAGGATATTACAACCCAAGAGGAACGGGAAGAGGAATTCATTTCAACACCAGACAAAATGCTCAAAACCAAAATCCATCCAACTTTGCTGCTTCTAAATCAAATATTTACTCTCCCACTCAAGGATCAAATCAAGGTAACAATATACAATGTCAAATCTGTGGAAAGACTAACCACTCGGCTTTGAAATGTTGGAATAGATTTGATCATGCATATCAATCTGAAGAAATTACAAAAGCACTAGCAGCGTTGACATTTTAAGATGGAATGGACTCAAATATGTATGCTGACTCTGGTGCCACCTCTTACGTTGTCAATGATTTAGGTAAAGTTTCTAAAACTCAGAGATATATAGGGAGGAAAAACTATATGTTGGTAATGGAAATGGATTAAATATTACTCACATTGTCATGGAAAACTAAAATCTAGCCATGGTGATTTAGAATTCAATAATGTGTTAGTTGTTGATAAGTCTTGAATTTTAGGAAAAAGTGTTTACAAATTGTGCATGTTTAGTGTCTTCTGAAATTAAATACGTGTGTTTCATGCATTGAATCCGGTATAAATGGGACTATTTCTTAGAAAAGACTTGTACACTTGGTTTTGGATAGATAGATGGAATTATGAGGAAATATATATGTTTTATAGGGGAAAAAGGAAGGAAAAAGTAGCAATGCACCTGCTACATATGACACAGAGGCCATCTTAAAGCTAGCAGCGATGCGTCTGGCGCATCGCCAACACTCCAGAAACTTCAACAATGCGCTTGGCGCATTCTCCACCTACAAATAA

mRNA sequence

ATGATCGAAGGATTAGATACAACTTATAAGGTATGGAAATCTTTGGAAGAGACTCCCCTAGCCATTACCAAAGAAAATGAAATTCATATCAGTGAAGTTCTACACTATTTGAGGAAGGGGAATCTATCACTTGATGAATACATCAAGAAATTCAAATCCTTGAGTGACAAGCTTGCTGCCATGAAGAAACCATTGGATGATTTAAGCAAGGGGAAAAAGGAAGGAAAAAGTAGCAATGCACCTGCTACATATGACACAGAGGCCATCTTAAAGCTAGCAGCGATGCGTCTGGCGCATCGCCAACACTCCAGAAACTTCAACAATGCGCTTGGCGCATTCTCCACCTACAAATAA

Coding sequence (CDS)

ATGATCGAAGGATTAGATACAACTTATAAGGTATGGAAATCTTTGGAAGAGACTCCCCTAGCCATTACCAAAGAAAATGAAATTCATATCAGTGAAGTTCTACACTATTTGAGGAAGGGGAATCTATCACTTGATGAATACATCAAGAAATTCAAATCCTTGAGTGACAAGCTTGCTGCCATGAAGAAACCATTGGATGATTTAAGCAAGGGGAAAAAGGAAGGAAAAAGTAGCAATGCACCTGCTACATATGACACAGAGGCCATCTTAAAGCTAGCAGCGATGCGTCTGGCGCATCGCCAACACTCCAGAAACTTCAACAATGCGCTTGGCGCATTCTCCACCTACAAATAA

Protein sequence

MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAAMKKPLDDLSKGKKEGKSSNAPATYDTEAILKLAAMRLAHRQHSRNFNNALGAFSTYK
Homology
BLAST of Moc07g20320 vs. NCBI nr
Match: XP_022154021.1 (uncharacterized protein LOC111021379 [Momordica charantia] >XP_022154022.1 uncharacterized protein LOC111021379 [Momordica charantia])

HSP 1 Score: 92.8 bits (229), Expect = 2.1e-15
Identity = 44/70 (62.86%), Postives = 58/70 (82.86%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           ++EG +T  +VW SLEE  L +TKENEIH++E L  L+KG+LS+DEYI+KFK+L D+L A
Sbjct: 97  LLEGTETAKEVWHSLEELLLTMTKENEIHLNEALLTLKKGSLSMDEYIRKFKNLCDRLXA 156

Query: 61  MKKPLDDLSK 71
           MKKPLDDL+K
Sbjct: 157 MKKPLDDLTK 166

BLAST of Moc07g20320 vs. NCBI nr
Match: KAA0051410.1 (putative leucine-rich repeat receptor-like protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 92.8 bits (229), Expect = 2.1e-15
Identity = 47/69 (68.12%), Postives = 56/69 (81.16%), Query Frame = 0

Query: 1  MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
          M+EGL+  ++VW SLEE  L+ITKENEIHISE LH L+KG L L+EYIK F  LS+KLA 
Sbjct: 1  MLEGLENAHQVWNSLEEILLSITKENEIHISESLHALKKGLLILEEYIKCFMVLSEKLAV 60

Query: 61 MKKPLDDLS 70
          MKKPLDDL+
Sbjct: 61 MKKPLDDLT 69

BLAST of Moc07g20320 vs. NCBI nr
Match: RVX03989.1 (hypothetical protein CK203_021715 [Vitis vinifera])

HSP 1 Score: 74.7 bits (182), Expect = 5.9e-10
Identity = 34/70 (48.57%), Postives = 50/70 (71.43%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           +++G  T+Y VW SLEE  L +TKE E+ ++  L  L+KG  SLDEY+++FK + D LAA
Sbjct: 38  LLDGTKTSYDVWNSLEEKLLPMTKEKEVQLTNKLQGLKKGTRSLDEYLREFKGICDALAA 97

Query: 61  MKKPLDDLSK 71
           ++KP+ DL K
Sbjct: 98  VRKPVSDLDK 107

BLAST of Moc07g20320 vs. NCBI nr
Match: CAN74381.1 (hypothetical protein VITISV_007944 [Vitis vinifera])

HSP 1 Score: 71.2 bits (173), Expect = 6.5e-09
Identity = 32/70 (45.71%), Postives = 49/70 (70.00%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           +++G +T Y VW SL E  L +TKE E+ ++  L  ++KG  SLDEY+++FK + D LAA
Sbjct: 97  LLDGTETAYDVWNSLGEKLLPMTKEKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAA 156

Query: 61  MKKPLDDLSK 71
           ++KP+ DL K
Sbjct: 157 VRKPVSDLDK 166

BLAST of Moc07g20320 vs. NCBI nr
Match: RVW43526.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 71.2 bits (173), Expect = 6.5e-09
Identity = 32/70 (45.71%), Postives = 49/70 (70.00%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           +++G +T Y VW SL E  L +TKE E+ ++  L  ++KG  SLDEY+++FK + D LAA
Sbjct: 142 LLDGTETAYDVWNSLGEKLLPMTKEKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAA 201

Query: 61  MKKPLDDLSK 71
           ++KP+ DL K
Sbjct: 202 VRKPVSDLDK 211

BLAST of Moc07g20320 vs. ExPASy TrEMBL
Match: A0A6J1DMG5 (uncharacterized protein LOC111021379 OS=Momordica charantia OX=3673 GN=LOC111021379 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.0e-15
Identity = 44/70 (62.86%), Postives = 58/70 (82.86%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           ++EG +T  +VW SLEE  L +TKENEIH++E L  L+KG+LS+DEYI+KFK+L D+L A
Sbjct: 97  LLEGTETAKEVWHSLEELLLTMTKENEIHLNEALLTLKKGSLSMDEYIRKFKNLCDRLXA 156

Query: 61  MKKPLDDLSK 71
           MKKPLDDL+K
Sbjct: 157 MKKPLDDLTK 166

BLAST of Moc07g20320 vs. ExPASy TrEMBL
Match: A0A5A7U8B5 (Putative leucine-rich repeat receptor-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55G001530 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.0e-15
Identity = 47/69 (68.12%), Postives = 56/69 (81.16%), Query Frame = 0

Query: 1  MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
          M+EGL+  ++VW SLEE  L+ITKENEIHISE LH L+KG L L+EYIK F  LS+KLA 
Sbjct: 1  MLEGLENAHQVWNSLEEILLSITKENEIHISESLHALKKGLLILEEYIKCFMVLSEKLAV 60

Query: 61 MKKPLDDLS 70
          MKKPLDDL+
Sbjct: 61 MKKPLDDLT 69

BLAST of Moc07g20320 vs. ExPASy TrEMBL
Match: A0A438J4W0 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_021715 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.8e-10
Identity = 34/70 (48.57%), Postives = 50/70 (71.43%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           +++G  T+Y VW SLEE  L +TKE E+ ++  L  L+KG  SLDEY+++FK + D LAA
Sbjct: 38  LLDGTKTSYDVWNSLEEKLLPMTKEKEVQLTNKLQGLKKGTRSLDEYLREFKGICDALAA 97

Query: 61  MKKPLDDLSK 71
           ++KP+ DL K
Sbjct: 98  VRKPVSDLDK 107

BLAST of Moc07g20320 vs. ExPASy TrEMBL
Match: A0A2K3PNP5 (Copia-like polyprotein (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g013628 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.1e-09
Identity = 36/70 (51.43%), Postives = 47/70 (67.14%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           MI G DT Y +W SL E  L  T++ E  +   L+ L KGNLSLDEYI+KFK L +KL+A
Sbjct: 915 MILGGDTAYYIWSSLHEQLLPNTEDGEAQLKNSLYALSKGNLSLDEYIRKFKELCNKLSA 974

Query: 61  MKKPLDDLSK 71
           + KP+ D+ K
Sbjct: 975 IGKPVSDVDK 984

BLAST of Moc07g20320 vs. ExPASy TrEMBL
Match: A5AU03 (Reverse transcriptase Ty1/copia-type domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_013750 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.1e-09
Identity = 32/70 (45.71%), Postives = 49/70 (70.00%), Query Frame = 0

Query: 1   MIEGLDTTYKVWKSLEETPLAITKENEIHISEVLHYLRKGNLSLDEYIKKFKSLSDKLAA 60
           +++G +T Y VW SL E  L +TKE E+ ++  L  ++KG  SLDEY+++FK + D LAA
Sbjct: 523 LLDGTETAYDVWNSLGEKLLPMTKEKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAA 582

Query: 61  MKKPLDDLSK 71
           ++KP+ DL K
Sbjct: 583 VRKPVSDLDK 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154021.12.1e-1562.86uncharacterized protein LOC111021379 [Momordica charantia] >XP_022154022.1 uncha... [more]
KAA0051410.12.1e-1568.12putative leucine-rich repeat receptor-like protein kinase [Cucumis melo var. mak... [more]
RVX03989.15.9e-1048.57hypothetical protein CK203_021715 [Vitis vinifera][more]
CAN74381.16.5e-0945.71hypothetical protein VITISV_007944 [Vitis vinifera][more]
RVW43526.16.5e-0945.71Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DMG51.0e-1562.86uncharacterized protein LOC111021379 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A5A7U8B51.0e-1568.12Putative leucine-rich repeat receptor-like protein kinase OS=Cucumis melo var. m... [more]
A0A438J4W02.8e-1048.57Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_021715 PE=4 SV=1[more]
A0A2K3PNP53.1e-0951.43Copia-like polyprotein (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g013628... [more]
A5AU033.1e-0945.71Reverse transcriptase Ty1/copia-type domain-containing protein OS=Vitis vinifera... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 2..68
e-value: 8.2E-7
score: 28.9
NoneNo IPR availablePANTHERPTHR47481:SF2SUBFAMILY NOT NAMEDcoord: 1..70
NoneNo IPR availablePANTHERPTHR47481FAMILY NOT NAMEDcoord: 1..70

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc07g20320.1Moc07g20320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0016310 phosphorylation
biological_process GO:0044238 primary metabolic process
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0016301 kinase activity