Moc07g04550 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g04550
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr7: 3923950 .. 3925194 (+)
RNA-Seq Expression	Moc07g04550
Synteny	Moc07g04550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGCGGACTTCATTAGGGGCAACTCACCACAAGACCCCAAGGAGCGCAGAAAGTTGGCAAGGCGGGCAGCTCGGTTCGTGGTCTGAGATGGGGCATTGTACCGACGTGGCTTTTCCCTGCCTCTGTTGAGATGCCTAACCCCTGAAGAGGGCCTGTACGTCCTCAGAGAGATCCACGAAGGAGTGTGCGGCAATCACTCAGGCGCCCGGTCGCTGTCAGCCAAAGTGATCCGACAAGGATACTATTGCCCGACCCTCAGCCAGGACGCCAAGAAGTTCGTTAGAACTTGCGACAATTGCCAACGCTACGGAACCATAATCCACCAACCTCCCGAGCTGCTCACCCCCATCTCGGCCCCGTGGCCATTCGCGCAGTGGGGGGTAGATATCATTGGTCCTTTCCCTTTGGGCAAGGGCCAGACCAAGTTCGCTGTGGTTGCTGTGGATTACTTCACCAAGTGGGCCGAGGCCGAGGCGCTCTCCCACATAACGGAATCCAGAGTCACGTCCTTCGTATGGACGAATATCATATGTCGCTTTGGTATACCGTAGGCCATCGTGACAGACAATGGGAAGCAGTTTGACAACGCCAAGTTCAAAGACTTTTGCAGCAAACTTGGCATAAGTCATCTTAGCTCGTCCCCCGCACATCCGCAAGCAAATGGACAGGTGGAGGCAGTCAACAAGATCATCAAGCGAGGCATCAAACTTAGACTGGACTCCAAGAAAGGCAGGTGGACCGAGGAGCTACCAGAGGTTCTATGGTTGTACCAGACCACCAAACGAGAGTCGACGGGTGAGACCCCGTTCTCCCTGGCCTTCGGCTCCGAAGCTGTAGTCCCGGTTGAGATCGGCATGCCATCTGACAGAGTAGAGCATTACGAACCTACGACAAATGAGGAAGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGAAGAGCAATGGCCCAGCTACGCCTGGCGGAATATTAGGGCAGAATGGCCAGACATTACAATGCTCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAACGCATGTGGGTGCCCTTGATTCGGCCTGGGAGGGCCCGTTTGAGGTCAAGGGCATAGTCCGACCTGGGACGTACGTATTGGCCGATCTACAAGGAGACGTCCTCGCGCACCCGTGGAACGCGGAGCACCTAAAGCGTTATTATCCTTGA

mRNA sequence

ATGGAGATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGCGGACTTCATTAGGGGCAACTCACCACAAGACCCCAAGGAGCGCAGAAAGTTGGCAAGGCGGGCAGCTCGGTTCGTGGGCAGAATGGCCAGACATTACAATGCTCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAACGCATGTGGGTGCCCTTGATTCGGCCTGGGAGGGCCCGTTTGAGGTCAAGGGCATAGTCCGACCTGGGACGTACGTATTGGCCGATCTACAAGGAGACGTCCTCGCGCACCCGTGGAACGCGGAGCACCTAAAGCGTTATTATCCTTGA

Coding sequence (CDS)

ATGGAGATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGCGGACTTCATTAGGGGCAACTCACCACAAGACCCCAAGGAGCGCAGAAAGTTGGCAAGGCGGGCAGCTCGGTTCGTGGGCAGAATGGCCAGACATTACAATGCTCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAACGCATGTGGGTGCCCTTGATTCGGCCTGGGAGGGCCCGTTTGAGGTCAAGGGCATAGTCCGACCTGGGACGTACGTATTGGCCGATCTACAAGGAGACGTCCTCGCGCACCCGTGGAACGCGGAGCACCTAAAGCGTTATTATCCTTGA

Protein sequence

MEIGAPESSWMDPIADFIRGNSPQDPKERRKLARRAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDSAWEGPFEVKGIVRPGTYVLADLQGDVLAHPWNAEHLKRYYP

Homology

[Close]

BLAST of Moc07g04550 vs. NCBI nr
Match: XP_022159003.1 (uncharacterized protein LOC111025448 [Momordica charantia])

HSP 1 Score: 130.6 bits (327), Expect = 8.9e-27
Identity = 59/89 (66.29%), Postives = 69/89 (77.53%), Query Frame = 0


Query: 28  ERRKLARRAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDSAWEGPFEVKGIV 87
           +R     R      RMARHYN R++PR+FQVGHLV  ++Q+HVG LD +WE  FEVKGIV
Sbjct: 541 KRETAQLRLVECQNRMARHYNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIV 600

Query: 88  RPGTYVLADLQGDVLAHPWNAEHLKRYYP 117
           RPGTY+LADL+  VLAHPWNAEHLKRYYP
Sbjct: 601 RPGTYMLADLERSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc07g04550 vs. NCBI nr
Match: XP_022156070.1 (uncharacterized protein K02A2.6-like [Momordica charantia])

HSP 1 Score: 110.5 bits (275), Expect = 9.6e-21
Identity = 51/79 (64.56%), Postives = 67/79 (84.81%), Query Frame = 0


Query: 28  ERRKLAR-RAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDSAWEGPFEVKGI 87
           E+R+ A+ R A +  +MARHYN+RVR R+FQ+G+LVLR+VQ HV  L+ +WEGPFEVKGI
Sbjct: 272 EKREAAQLRLAEYQNQMARHYNSRVRSRSFQIGYLVLRKVQKHVEVLEPSWEGPFEVKGI 331

Query: 88  VRPGTYVLADLQGDVLAHP 106
           VRPGTY+LADL+G +L+HP
Sbjct: 332 VRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc07g04550 vs. NCBI nr
Match: XP_022144032.1 (uncharacterized protein LOC111013824 [Momordica charantia])

HSP 1 Score: 102.8 bits (255), Expect = 2.0e-18
Identity = 42/55 (76.36%), Postives = 51/55 (92.73%), Query Frame = 0


Query: 62  VLRRVQTHVGALDSAWEGPFEVKGIVRPGTYVLADLQGDVLAHPWNAEHLKRYYP 117
           V +++Q+HVG LD +WEGPFEVKGI+R GTY+LADL+G+VLAHPWNAEHLKRYYP
Sbjct: 414 VEQKIQSHVGTLDPSWEGPFEVKGIIRFGTYMLADLEGNVLAHPWNAEHLKRYYP 468

[Close]

BLAST of Moc07g04550 vs. NCBI nr
Match: XP_022144032.1 (uncharacterized protein LOC111013824 [Momordica charantia])

HSP 1 Score: 55.1 bits (131), Expect = 4.8e-04
Identity = 23/42 (54.76%), Postives = 31/42 (73.81%), Query Frame = 0


Query: 1   MEIGAPESSWMDPIADFIRGNSPQDPKERRKLARRAARFVGR 43
           ME+  PE SWMDPI +FI+ N PQD K  +K++R+ ARF+ R
Sbjct: 150 MEVETPEPSWMDPIVEFIKRNPPQDSKAHKKMSRKVARFILR 191

HSP 2 Score: 102.1 bits (253), Expect = 3.4e-18
Identity = 46/81 (56.79%), Postives = 58/81 (71.60%), Query Frame = 0


Query: 35  RAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDSAWEGPFEVKGIVRPGTYVL 94
           R A +  RMAR+YNARV+ R F+   LVL++V  HVGAL+  WEGP+ V  I+RP  Y+L
Sbjct: 764 RLAEYQNRMARYYNARVKVRTFKPSDLVLKKVMQHVGALEPNWEGPYRVLKILRPRAYLL 823

Query: 95  ADLQGDVLAHPWNAEHLKRYY 116
           +DL G  L HPWNAEHL+ YY
Sbjct: 824 SDLNGRQLPHPWNAEHLRVYY 844

[Close]

BLAST of Moc07g04550 vs. NCBI nr
Match: XP_022156561.1 (uncharacterized protein LOC111023436 [Momordica charantia])

HSP 1 Score: 95.1 bits (235), Expect = 4.2e-16
Identity = 46/95 (48.42%), Postives = 63/95 (66.32%), Query Frame = 0


Query: 27  KERRKLAR-RAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHV-----GALDSAWEGP 86
           +E R+ AR R A +  R+AR+YN++V+ R F+ G LVLR++  +      GA    WEGP
Sbjct: 131 EEHRETARLRTAEYKNRIARYYNSKVKKRDFKEGDLVLRKIMPNTKDSTSGAFRPTWEGP 190

Query: 87  FEVKGIVRPGTYVLADLQGDVLAHPWNAEHLKRYY 116
           ++V  +VR GTY L D QG  L HPW+A+HLKRYY
Sbjct: 191 YKVTKVVRTGTYALEDQQGKALLHPWHAQHLKRYY 225

[Close]

BLAST of Moc07g04550 vs. ExPASy TrEMBL
Match: A0A6J1DXL9 (uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025448 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 4.3e-27
Identity = 59/89 (66.29%), Postives = 69/89 (77.53%), Query Frame = 0


Query: 28  ERRKLARRAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDSAWEGPFEVKGIV 87
           +R     R      RMARHYN R++PR+FQVGHLV  ++Q+HVG LD +WE  FEVKGIV
Sbjct: 541 KRETAQLRLVECQNRMARHYNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIV 600

Query: 88  RPGTYVLADLQGDVLAHPWNAEHLKRYYP 117
           RPGTY+LADL+  VLAHPWNAEHLKRYYP
Sbjct: 601 RPGTYMLADLERSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc07g04550 vs. ExPASy TrEMBL
Match: A0A6J1DP99 (uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023037 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 4.6e-21
Identity = 51/79 (64.56%), Postives = 67/79 (84.81%), Query Frame = 0


Query: 28  ERRKLAR-RAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDSAWEGPFEVKGI 87
           E+R+ A+ R A +  +MARHYN+RVR R+FQ+G+LVLR+VQ HV  L+ +WEGPFEVKGI
Sbjct: 272 EKREAAQLRLAEYQNQMARHYNSRVRSRSFQIGYLVLRKVQKHVEVLEPSWEGPFEVKGI 331

Query: 88  VRPGTYVLADLQGDVLAHP 106
           VRPGTY+LADL+G +L+HP
Sbjct: 332 VRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc07g04550 vs. ExPASy TrEMBL
Match: A0A6J1CSJ1 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013824 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 9.7e-19
Identity = 42/55 (76.36%), Postives = 51/55 (92.73%), Query Frame = 0


Query: 62  VLRRVQTHVGALDSAWEGPFEVKGIVRPGTYVLADLQGDVLAHPWNAEHLKRYYP 117
           V +++Q+HVG LD +WEGPFEVKGI+R GTY+LADL+G+VLAHPWNAEHLKRYYP
Sbjct: 414 VEQKIQSHVGTLDPSWEGPFEVKGIIRFGTYMLADLEGNVLAHPWNAEHLKRYYP 468

[Close]

BLAST of Moc07g04550 vs. ExPASy TrEMBL
Match: A0A6J1CSJ1 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013824 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.3e-04
Identity = 23/42 (54.76%), Postives = 31/42 (73.81%), Query Frame = 0


Query: 1   MEIGAPESSWMDPIADFIRGNSPQDPKERRKLARRAARFVGR 43
           ME+  PE SWMDPI +FI+ N PQD K  +K++R+ ARF+ R
Sbjct: 150 MEVETPEPSWMDPIVEFIKRNPPQDSKAHKKMSRKVARFILR 191

HSP 2 Score: 102.1 bits (253), Expect = 1.6e-18
Identity = 46/81 (56.79%), Postives = 58/81 (71.60%), Query Frame = 0


Query: 35  RAARFVGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDSAWEGPFEVKGIVRPGTYVL 94
           R A +  RMAR+YNARV+ R F+   LVL++V  HVGAL+  WEGP+ V  I+RP  Y+L
Sbjct: 764 RLAEYQNRMARYYNARVKVRTFKPSDLVLKKVMQHVGALEPNWEGPYRVLKILRPRAYLL 823

Query: 95  ADLQGDVLAHPWNAEHLKRYY 116
           +DL G  L HPWNAEHL+ YY
Sbjct: 824 SDLNGRQLPHPWNAEHLRVYY 844

[Close]

BLAST of Moc07g04550 vs. ExPASy TrEMBL
Match: A0A2N9F996 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11236 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.4e-17
Identity = 47/93 (50.54%), Postives = 60/93 (64.52%), Query Frame = 0


Query: 29   RRKLARRAARFVGRMARHYNARVRPRAFQVGHLVLRRV-----QTHVGALDSAWEGPFEV 88
            R K   R  R+  +MARHYN++V+PR   +G LVLR+V         G L   WEGP++V
Sbjct: 1848 REKAEARMKRYQEKMARHYNSKVKPRQLSIGDLVLRKVTLATRNPSEGKLGPNWEGPYKV 1907

Query: 89   KGIVRPGTYVLADLQGDVLAHPWNAEHLKRYYP 117
              + RPGTY L D+ G  L+HPWNAEHL+RYYP
Sbjct: 1908 IEVRRPGTYHLEDMNGRRLSHPWNAEHLRRYYP 1940

The following BLAST results are available for this feature:

BLAST of Moc07g04550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159003.1	8.9e-27	66.29	uncharacterized protein LOC111025448 [Momordica charantia]	[more]
XP_022156070.1	9.6e-21	64.56	uncharacterized protein K02A2.6-like [Momordica charantia]	[more]
XP_022144032.1	2.0e-18	76.36	uncharacterized protein LOC111013824 [Momordica charantia]	[more]
XP_022144032.1	4.8e-04	54.76	uncharacterized protein LOC111013824 [Momordica charantia]	[more]
XP_022156561.1	4.2e-16	48.42	uncharacterized protein LOC111023436 [Momordica charantia]	[more]

BLAST of Moc07g04550 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc07g04550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DXL9	4.3e-27	66.29	uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DP99	4.6e-21	64.56	uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CSJ1	9.7e-19	76.36	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013824 PE=4 SV=1	[more]
A0A6J1CSJ1	2.3e-04	54.76	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013824 PE=4 SV=1	[more]
A0A2N9F996	1.4e-17	50.54	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11236 PE=4 SV=1	[more]

BLAST of Moc07g04550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g04550.1	Moc07g04550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity