Moc07g04130 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc07g04130
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionrRNA N-glycosidase
Locationchr7: 3579721 .. 3580647 (+)
RNA-Seq ExpressionMoc07g04130
SyntenyMoc07g04130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGTGTGAGGTCATCTGAACCCTTCATTCAACCCCTTCCCTTCAATGATATTGGCATTCATGGTGTGCATGCTAATGGTGCTGTGAATGCTCCCAATTCTAATGTCAGTTTCTCTCTGTTGGGTGCTACTGGCAAAACTTACCAACAGTTCATACAGAATTTGCGTAACGCTCTCACAATCAACTCCAAAATAGTGTACAACATACCGGTGCTAGCGGCCACGGCCTTGAGTTCGGCGCGCTTCATACTAGTCCATCTCACCAACTACAAAAATGAGACCATCACTGTTGCAATTGACGTAGTTAACGTTTATATCGTCGCATATCAAGCTGGAAACAAAGCCTACTTTCTGAAAGATGCCTCAAAAGAAGCAAGAGATGTGCTATTCAAAGGCATCAAGCAAGAAATACTTCCTTACAAAGGCAATTACGACGGCCTCGAGACCGCTGCAGGAAAGATTTCAAGAGAGAAAATTGATCTTGGATTCTCTGAACTAGGCAGTGCTATCGGCAACATGTACCACTATAACGCTGGTACTTCTGTCCCTAGAGCATTCATTGTGATGATTCAGACGATTTCAGAAGCGGCAAGATTTAAGTACATCGAGACAAAAGTTTCACAGAATGTCGAGACTAAATTTAAGCCAGACCCAGGATTCTTAAGCTTAGAGAATCGTTGGTCTGATCTATCTGAACAAGTACAAATTGCACAGAATCGTAAAGGAGAATTTGCTCGTCCTATCGAAGTTCGAAGTGTTACCAACAAACCAATTCTTGTAACGAATGTGAAGTCCAAAGTTGTGGAAGGCATGGCACTGTTGCTTTACAGCAAGCCGAGGGGCAATGGAGATCTAATGGAACTGATGGAAGAGTTCAATAAAGAATGGGAAAATGGTCGAGGTGAATTTGGAATTGACTTCTGA

mRNA sequence

ATGGAGTGTGTGAGGTCATCTGAACCCTTCATTCAACCCCTTCCCTTCAATGATATTGGCATTCATGGTGTGCATGCTAATGGTGCTGTGAATGCTCCCAATTCTAATGTCAGTTTCTCTCTGTTGGGTGCTACTGGCAAAACTTACCAACAGTTCATACAGAATTTGCGTAACGCTCTCACAATCAACTCCAAAATAGTGTACAACATACCGGTGCTAGCGGCCACGGCCTTGAGTTCGGCGCGCTTCATACTAGTCCATCTCACCAACTACAAAAATGAGACCATCACTGTTGCAATTGACGTAGTTAACGTTTATATCGTCGCATATCAAGCTGGAAACAAAGCCTACTTTCTGAAAGATGCCTCAAAAGAAGCAAGAGATGTGCTATTCAAAGGCATCAAGCAAGAAATACTTCCTTACAAAGGCAATTACGACGGCCTCGAGACCGCTGCAGGAAAGATTTCAAGAGAGAAAATTGATCTTGGATTCTCTGAACTAGGCAGTGCTATCGGCAACATGTACCACTATAACGCTGGTACTTCTGTCCCTAGAGCATTCATTGTGATGATTCAGACGATTTCAGAAGCGGCAAGATTTAAGTACATCGAGACAAAAGTTTCACAGAATGTCGAGACTAAATTTAAGCCAGACCCAGGATTCTTAAGCTTAGAGAATCGTTGGTCTGATCTATCTGAACAAGTACAAATTGCACAGAATCGTAAAGGAGAATTTGCTCGTCCTATCGAAGTTCGAAGTGTTACCAACAAACCAATTCTTGTAACGAATGTGAAGTCCAAAGTTGTGGAAGGCATGGCACTGTTGCTTTACAGCAAGCCGAGGGGCAATGGAGATCTAATGGAACTGATGGAAGAGTTCAATAAAGAATGGGAAAATGGTCGAGGTGAATTTGGAATTGACTTCTGA

Coding sequence (CDS)

ATGGAGTGTGTGAGGTCATCTGAACCCTTCATTCAACCCCTTCCCTTCAATGATATTGGCATTCATGGTGTGCATGCTAATGGTGCTGTGAATGCTCCCAATTCTAATGTCAGTTTCTCTCTGTTGGGTGCTACTGGCAAAACTTACCAACAGTTCATACAGAATTTGCGTAACGCTCTCACAATCAACTCCAAAATAGTGTACAACATACCGGTGCTAGCGGCCACGGCCTTGAGTTCGGCGCGCTTCATACTAGTCCATCTCACCAACTACAAAAATGAGACCATCACTGTTGCAATTGACGTAGTTAACGTTTATATCGTCGCATATCAAGCTGGAAACAAAGCCTACTTTCTGAAAGATGCCTCAAAAGAAGCAAGAGATGTGCTATTCAAAGGCATCAAGCAAGAAATACTTCCTTACAAAGGCAATTACGACGGCCTCGAGACCGCTGCAGGAAAGATTTCAAGAGAGAAAATTGATCTTGGATTCTCTGAACTAGGCAGTGCTATCGGCAACATGTACCACTATAACGCTGGTACTTCTGTCCCTAGAGCATTCATTGTGATGATTCAGACGATTTCAGAAGCGGCAAGATTTAAGTACATCGAGACAAAAGTTTCACAGAATGTCGAGACTAAATTTAAGCCAGACCCAGGATTCTTAAGCTTAGAGAATCGTTGGTCTGATCTATCTGAACAAGTACAAATTGCACAGAATCGTAAAGGAGAATTTGCTCGTCCTATCGAAGTTCGAAGTGTTACCAACAAACCAATTCTTGTAACGAATGTGAAGTCCAAAGTTGTGGAAGGCATGGCACTGTTGCTTTACAGCAAGCCGAGGGGCAATGGAGATCTAATGGAACTGATGGAAGAGTTCAATAAAGAATGGGAAAATGGTCGAGGTGAATTTGGAATTGACTTCTGA

Protein sequence

MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEWENGRGEFGIDF
Homology
BLAST of Moc07g04130 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 607.1 bits (1564), Expect = 8.6e-170
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL 60
           MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL
Sbjct: 1   MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL 60

Query: 61  TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK 120
           TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK
Sbjct: 61  TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK 120

Query: 121 DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG 180
           DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG
Sbjct: 121 DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG 180

Query: 181 TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN 240
           TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN
Sbjct: 181 TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN 240

Query: 241 RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEWENG 300
           RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEWENG
Sbjct: 241 RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEWENG 300

Query: 301 RGEFGIDF 309
           RGEFGIDF
Sbjct: 301 RGEFGIDF 308

BLAST of Moc07g04130 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 392.1 bits (1006), Expect = 4.4e-105
Identity = 212/313 (67.73%), Postives = 248/313 (79.23%), Query Frame = 0

Query: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60
           MEC+ S++  IQ P P N     G H   A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECIGSTKDLIQAPFPLN----CGAHV--AIRAPRPSANANLSFSLLGATAQTYKQFISD 60

Query: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE IDLGFSEL S+IGNM+
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMF 180

Query: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240
           HYNAG SVP++FIVMIQ +SEA+RFKYIE +  +NVE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYNAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300
           Q AQ   G+F  P++VRSV+NKP+LV++V S VV+GMALLL +KP  NG + +LMEEF K
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNG-VSKLMEEFIK 300

Query: 301 EWENGRGEFGIDF 309
            WE  RG FGIDF
Sbjct: 301 -WEAARGHFGIDF 305

BLAST of Moc07g04130 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389.4 bits (999), Expect = 2.8e-104
Identity = 211/313 (67.41%), Postives = 249/313 (79.55%), Query Frame = 0

Query: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60
           MEC+ S++  IQ P P N     G HA  A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECIGSTQDLIQAPFPLN----CGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFIND 60

Query: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE I+LGFSEL S+IGNM+
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180

Query: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240
           HYNAG SVPR+FIVMIQ +SEA+RFKYIET+  + VE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300
           Q AQ   G+F  P++VRSV+NKP+LV++V S VV+G+ALLL +KP  NG + +LMEEF K
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNG-VSKLMEEFIK 300

Query: 301 EWENGRGEFGIDF 309
            WE  RG FGI+F
Sbjct: 301 -WEAARGAFGINF 305

BLAST of Moc07g04130 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 387.1 bits (993), Expect = 1.4e-103
Identity = 210/313 (67.09%), Postives = 247/313 (78.91%), Query Frame = 0

Query: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60
           MEC+ S++  IQ P P N     G HA  A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECIGSTQDLIQAPFPLN----CGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFIND 60

Query: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE I+LGFSEL S+IGNM+
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180

Query: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240
           HY AG SVPR+FIVMIQ +SEA+RFKYIET+  + VE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300
           Q AQ   G+F  P++VRSV+NKP+LV++V S VV+G+ALLL +KP  NG + +LMEEF  
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNG-VSKLMEEF-I 300

Query: 301 EWENGRGEFGIDF 309
            WE  RG FGIDF
Sbjct: 301 HWEAARGHFGIDF 305

BLAST of Moc07g04130 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 386.7 bits (992), Expect = 1.8e-103
Identity = 211/313 (67.41%), Postives = 246/313 (78.59%), Query Frame = 0

Query: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60
           MEC+ S++  IQ P P N     G HA  A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECIGSTQDLIQAPFPLN----CGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFIND 60

Query: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120
           LRNALT+ S  VY IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRNALTVQSHKVYGIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AG ISRE I+LGFSEL S+IGNM+
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMF 180

Query: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240
           HYNAG SVPR+FIVMIQ +SEA+RFKYIET+  + VE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300
           Q AQ   G+F RP++VRSV+NKPI VT+V S VV+G+ALLL +KP  NG + +LME+F  
Sbjct: 241 QKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNG-VSKLMEQF-I 300

Query: 301 EWENGRGEFGIDF 309
            WE  RG FGIDF
Sbjct: 301 HWEAARGAFGIDF 305

BLAST of Moc07g04130 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.6e-89
Identity = 172/261 (65.90%), Postives = 206/261 (78.93%), Query Frame = 0

Query: 19  IGIHGVHA--NGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAAT 78
           IG + V A   GA    + +++FSL+GATG TY+ FI+NLR  LT+ +  VY+IPVL   
Sbjct: 4   IGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNA 63

Query: 79  ALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQ 138
           A   ARF LV LTNY  E++TVA+DVVNVY+VAY+AGN AYFL DAS EA +VLF GI  
Sbjct: 64  AAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINH 123

Query: 139 EILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISE 198
             LPY GNYDGLETAAG+ISRE I+LGFSE+ SAIGNM+ +N GTSVPRAFIV+IQT+SE
Sbjct: 124 VRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSE 183

Query: 199 AARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTN 258
           AARFKYIE +VS+NV TKFKPDP FLSL+N W  LSEQ+QIAQ R GEFARP+E+R+V+N
Sbjct: 184 AARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSN 243

Query: 259 KPILVTNVKSKVVEGMALLLY 278
            P  VTNV S VV+G+ALLLY
Sbjct: 244 TPTFVTNVNSPVVKGIALLLY 264

BLAST of Moc07g04130 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 125/256 (48.83%), Postives = 172/256 (67.19%), Query Frame = 0

Query: 30  VNAPNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLT 89
           V     +V+F L  AT KTY +FI++ R  L  + K VY+IP+L +T   S RFIL++LT
Sbjct: 18  VPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHK-VYDIPLLYSTISDSRRFILLNLT 77

Query: 90  NYKNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLE 149
           +Y  ETI+VAIDV NVY+VAY+  + +YF K++  EA ++LFKG ++  LPY GNY+ L+
Sbjct: 78  SYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQ 137

Query: 150 TAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQ 209
           TAA KI RE IDLG   L SAI  +++YNA  S P A +V+IQT +EAARFKYIE  V++
Sbjct: 138 TAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAK 197

Query: 210 NVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVV 269
            V T FKP+   +SLEN+WS LS+Q+ +AQN+ G+F  P+++   T +   VTNV S VV
Sbjct: 198 YVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVV 257

Query: 270 EGMALLLYSKPRGNGD 286
           +G   LL +      D
Sbjct: 258 KGNIKLLLNSRASTAD 270

BLAST of Moc07g04130 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.1e-57
Identity = 125/256 (48.83%), Postives = 171/256 (66.80%), Query Frame = 0

Query: 30  VNAPNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLT 89
           V     +V+F L  AT KTY +FI++ R  L  + K VY+IP+L +T   S RFIL+ LT
Sbjct: 18  VPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHK-VYDIPLLYSTISDSRRFILLDLT 77

Query: 90  NYKNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLE 149
           +Y  ETI+VAIDV NVY+VAY+  + +YF K++  EA ++LFKG ++  LPY GNY+ L+
Sbjct: 78  SYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQ 137

Query: 150 TAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQ 209
           TAA KI RE IDLG   L SAI  +++YNA  S P A +V+IQT +EAARFKYIE  V++
Sbjct: 138 TAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAK 197

Query: 210 NVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVV 269
            V T FKP+   +SLEN+WS LS+Q+ +AQN+ G+F  P+++   T +   VTNV S VV
Sbjct: 198 YVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVV 257

Query: 270 EGMALLLYSKPRGNGD 286
           +G   LL +      D
Sbjct: 258 KGNIKLLLNSRASTAD 270

BLAST of Moc07g04130 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 224.2 bits (570), Expect = 2.1e-57
Identity = 122/242 (50.41%), Postives = 163/242 (67.36%), Query Frame = 0

Query: 36  NVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNET 95
           NV F L  AT  +Y+ FI+NLR AL  + K VY+IPVL +T + S RFIL+ L NY  ++
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGK-VYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 96  ITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKI 155
           IT AIDV+NVYIVAY  G  +YF +    +A  +LFKG +Q  LPY GNY+ L+TAA K+
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 156 SREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKF 215
            RE I+LG   L SAI  ++HYNA  +   A +V+IQT SEAARF+YIE +++ NV TKF
Sbjct: 121 -RENIELGLPALDSAITTLFHYNA-EAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 216 KPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVV-EGMAL 275
           KP    +SLEN WS LS+Q+QIA+N+ G+F  P+ +       + +TNV S VV + + L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 239

Query: 276 LL 277
           LL
Sbjct: 241 LL 239

BLAST of Moc07g04130 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 206.5 bits (524), Expect = 4.5e-52
Identity = 118/248 (47.58%), Postives = 164/248 (66.13%), Query Frame = 0

Query: 32  APNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNY 91
           A   +VSF L GAT  +Y  FI NLR AL    K+ Y+IP+L +T   S R+ L+HLTNY
Sbjct: 20  AVEGDVSFRLSGATSSSYGVFISNLRKALPYERKL-YDIPLLRSTLPGSQRYALIHLTNY 79

Query: 92  KNETITVAIDVVNVYIVAYQAGNKAYFLKDAS-KEARDVLFKGIKQEI-LPYKGNYDGLE 151
            +ETI+VAIDV NVY++ Y+AG+ +YF  +AS  EA   +FK  K+++ LPY GNY+ L+
Sbjct: 80  ADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAKRKVTLPYSGNYERLQ 139

Query: 152 TAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQ 211
            AAGKI RE I LG   L SAI  +++YNA  S   A +V+IQ+ SEAAR+K+IE ++ +
Sbjct: 140 IAAGKI-RENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQSTSEAARYKFIEQQIGK 199

Query: 212 NVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVV 271
            V+  F P    +SLEN WS LS+Q+QIA    G+F  P+ + +  N+ + +TNV + VV
Sbjct: 200 RVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQNQRVTITNVDAGVV 259

Query: 272 -EGMALLL 277
              +ALLL
Sbjct: 260 TSNIALLL 264

BLAST of Moc07g04130 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.2e-170
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL 60
           MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL
Sbjct: 1   MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL 60

Query: 61  TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK 120
           TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK
Sbjct: 61  TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK 120

Query: 121 DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG 180
           DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG
Sbjct: 121 DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG 180

Query: 181 TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN 240
           TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN
Sbjct: 181 TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN 240

Query: 241 RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEWENG 300
           RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEWENG
Sbjct: 241 RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEWENG 300

Query: 301 RGEFGIDF 309
           RGEFGIDF
Sbjct: 301 RGEFGIDF 308

BLAST of Moc07g04130 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 2.1e-105
Identity = 212/313 (67.73%), Postives = 248/313 (79.23%), Query Frame = 0

Query: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60
           MEC+ S++  IQ P P N     G H   A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECIGSTKDLIQAPFPLN----CGAHV--AIRAPRPSANANLSFSLLGATAQTYKQFISD 60

Query: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE IDLGFSEL S+IGNM+
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMF 180

Query: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240
           HYNAG SVP++FIVMIQ +SEA+RFKYIE +  +NVE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYNAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300
           Q AQ   G+F  P++VRSV+NKP+LV++V S VV+GMALLL +KP  NG + +LMEEF K
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNG-VSKLMEEFIK 300

Query: 301 EWENGRGEFGIDF 309
            WE  RG FGIDF
Sbjct: 301 -WEAARGHFGIDF 305

BLAST of Moc07g04130 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 6.8e-104
Identity = 210/313 (67.09%), Postives = 247/313 (78.91%), Query Frame = 0

Query: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP----NSNVSFSLLGATGKTYQQFIQN 60
           MEC+ S++  IQ P P N     G HA  A+ AP    N+N+SFSLLGAT +TY+QFI +
Sbjct: 1   MECIGSTQDLIQAPFPLN----CGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFIND 60

Query: 61  LRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120
           LR ALT+NS  VY+IPVL ATA   ARFILV+LTNYK E+ITVAID V+VYIVAYQAGNK
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180
           AYFLKD SKEA DVLFKG K E L YKGNYD LE  AGKISRE I+LGFSEL S+IGNM+
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180

Query: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240
           HY AG SVPR+FIVMIQ +SEA+RFKYIET+  + VE+KFKPDPG+LSLENRWS+LSEQV
Sbjct: 181 HYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNK 300
           Q AQ   G+F  P++VRSV+NKP+LV++V S VV+G+ALLL +KP  NG + +LMEEF  
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNG-VSKLMEEF-I 300

Query: 301 EWENGRGEFGIDF 309
            WE  RG FGIDF
Sbjct: 301 HWEAARGHFGIDF 305

BLAST of Moc07g04130 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.3e-99
Identity = 185/250 (74.00%), Postives = 220/250 (88.00%), Query Frame = 0

Query: 32  APNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNY 91
           A  ++VSFS+LGAT +TY+QFI+NLR+ LTI S +VY IPVL +TA+ SARFILVHLTNY
Sbjct: 18  AAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHLTNY 77

Query: 92  KNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETA 151
             E+ITVAIDVVN+Y+VAY+AGN AYFL DAS EA +VLFKGIK   LPYKGNYDGLETA
Sbjct: 78  NTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGLETA 137

Query: 152 AGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNV 211
           AGKISREKIDLGFSE+ S+IGNM+H+N GTS+ RAFIV+IQ++ EAARFKYIE +VS+NV
Sbjct: 138 AGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSENV 197

Query: 212 ETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEG 271
           +TKFKPDP FLSLENRWSDLSEQ+QIAQ R+GEFAR IE+R+V+NKPI+VTNV S +V+G
Sbjct: 198 KTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIVKG 257

Query: 272 MALLLYSKPR 282
           +ALLLY K R
Sbjct: 258 IALLLYYKVR 267

BLAST of Moc07g04130 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 9.9e-95
Identity = 181/248 (72.98%), Postives = 218/248 (87.90%), Query Frame = 0

Query: 32  APNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNY 91
           A  ++VSFS+LG+T +TY+QFI+N+R+ALT+ S+IVY+IPVL ATA  SARF+LVHLTNY
Sbjct: 18  AAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTNY 77

Query: 92  KNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETA 151
            +E+I VAIDVVNVY+VAY+AGN AYFL DAS EA  VLF+GI    L YKGNYDGLETA
Sbjct: 78  NSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLETA 137

Query: 152 AGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNV 211
           A KISREKIDLGFSE+ S+IGNM+H+NAGTSVPRAFIV+IQ++SEAARFKYIE +VS+NV
Sbjct: 138 A-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSENV 197

Query: 212 ETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEG 271
           +T FKPDP FLSL+NRWSDLSEQ+QIAQ R GEFAR +E+R+V+NKPILVT+V S VV+G
Sbjct: 198 KTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVKG 257

Query: 272 MALLLYSK 280
           +ALLLY K
Sbjct: 258 IALLLYYK 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158191.18.6e-170100.00ribosome-inactivating protein bryodin II-like [Momordica charantia][more]
XP_022981562.14.4e-10567.73ribosome-inactivating protein bryodin II-like [Cucurbita maxima][more]
KAG7037232.12.8e-10467.41hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022926255.11.4e-10367.09ribosome-inactivating protein bryodin II-like [Cucurbita moschata][more]
XP_023523582.11.8e-10367.41ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P981841.6e-8965.90Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1[more]
P248171.2e-5748.83Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN... [more]
P293392.1e-5748.83Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV... [more]
P845312.1e-5750.41Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2[more]
P244784.5e-5247.58Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1E0934.2e-170100.00rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1[more]
A0A6J1IUB22.1e-10567.73rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1[more]
A0A6J1EKL06.8e-10467.09rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1[more]
A0A6J1DWK41.3e-9974.00rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1[more]
A0A6J1DVE79.9e-9572.98rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 187..207
score: 57.02
coord: 47..60
score: 43.23
coord: 157..171
score: 49.47
coord: 95..110
score: 56.09
coord: 219..236
score: 43.13
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 36..199
e-value: 2.5E-46
score: 159.4
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 46..236
e-value: 5.6E-35
score: 120.8
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 36..281
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 200..280
e-value: 1.3E-25
score: 91.2
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 36..281
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 191..207
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 37..278

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc07g04130.1Moc07g04130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0035821 modulation of process of other organism
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity