Moc07g03960 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g03960
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	rRNA N-glycosidase
Location	chr7: 3465914 .. 3466753 (+)
RNA-Seq Expression	Moc07g03960
Synteny	Moc07g03960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTGATAGTCTTTTGTTTGCTAATTATCGCCATCTTCCATGCAGCTCCTAGTGTTGAGGGCGATGTCAGCTTCACTTTGAAGGGTGTCACTAAAAAATCCTATGGAGCTTTCCTCAAATTCTTTAGAGGCGATGTTCCCGTTGAAAGTAAGACTGTGTTCGGTATACCTCTACTGCGAGCCACTGTTACGGGTGCAAATCGCTACACACGAATCCATCTTTCAGACCGTGATGATCATACCATCACCCTGGCTATAGACGTAACAAACGTTTATGTTCTCGGGTTTTACACCCCAAAGTGTGACGTATCCCACTTTTTTAACGATTCTGCTGCCATTCAAGCTAAGAAATATGTATTCACCAATTCAAAGGGGCAAATAACACTTCCGTTCGACGGCAGTTACTCAAAACTTCAAAGGGCTGGTGGCGATCGAGAAAAGCTCGACCTTGGACTCGTAGCCGTCAACTTTAACATTCCCAAGTTGTTTGACTACAGCTGCCCCAACTCCACGCAAACGCTAAGCGACGTTGCAAAGGCATGCGTTGTAGTCATTCAGACCATTTCTGAGGCTGCAAGATTTCAAAATATTGAGAACGCAGTTCAAAACAACATGCCACCAACTCGAGAAATTCTATCTTTGGAGAATAATTGGGGTGCTCTCTCGAAACAAGTTCAGCTGGCAGACCCTAATACTGGACGGTTTAAAGCTACCGTTCAGCTTATTGGCGAGACAGGTAATCCAGTTAATGTAATGGATGTCAATTCACCGTATGTAAAAGGCAACCTCCAGTTGCTGCTGAACCAACAGAATGTTCAACCTATGCCTACACTGTAA

mRNA sequence

ATGAAATTGATAGTCTTTTGTTTGCTAATTATCGCCATCTTCCATGCAGCTCCTAGTGTTGAGGGCGATGTCAGCTTCACTTTGAAGGGTGTCACTAAAAAATCCTATGGAGCTTTCCTCAAATTCTTTAGAGGCGATGTTCCCGTTGAAAGTAAGACTGTGTTCGGTATACCTCTACTGCGAGCCACTGTTACGGGTGCAAATCGCTACACACGAATCCATCTTTCAGACCGTGATGATCATACCATCACCCTGGCTATAGACGTAACAAACGTTTATGTTCTCGGGTTTTACACCCCAAAGTGTGACGTATCCCACTTTTTTAACGATTCTGCTGCCATTCAAGCTAAGAAATATGTATTCACCAATTCAAAGGGGCAAATAACACTTCCGTTCGACGGCAGTTACTCAAAACTTCAAAGGGCTGGTGGCGATCGAGAAAAGCTCGACCTTGGACTCGTAGCCGTCAACTTTAACATTCCCAAGTTGTTTGACTACAGCTGCCCCAACTCCACGCAAACGCTAAGCGACGTTGCAAAGGCATGCGTTGTAGTCATTCAGACCATTTCTGAGGCTGCAAGATTTCAAAATATTGAGAACGCAGTTCAAAACAACATGCCACCAACTCGAGAAATTCTATCTTTGGAGAATAATTGGGGTGCTCTCTCGAAACAAGTTCAGCTGGCAGACCCTAATACTGGACGGTTTAAAGCTACCGTTCAGCTTATTGGCGAGACAGGTAATCCAGTTAATGTAATGGATGTCAATTCACCGTATGTAAAAGGCAACCTCCAGTTGCTGCTGAACCAACAGAATGTTCAACCTATGCCTACACTGTAA

Coding sequence (CDS)

ATGAAATTGATAGTCTTTTGTTTGCTAATTATCGCCATCTTCCATGCAGCTCCTAGTGTTGAGGGCGATGTCAGCTTCACTTTGAAGGGTGTCACTAAAAAATCCTATGGAGCTTTCCTCAAATTCTTTAGAGGCGATGTTCCCGTTGAAAGTAAGACTGTGTTCGGTATACCTCTACTGCGAGCCACTGTTACGGGTGCAAATCGCTACACACGAATCCATCTTTCAGACCGTGATGATCATACCATCACCCTGGCTATAGACGTAACAAACGTTTATGTTCTCGGGTTTTACACCCCAAAGTGTGACGTATCCCACTTTTTTAACGATTCTGCTGCCATTCAAGCTAAGAAATATGTATTCACCAATTCAAAGGGGCAAATAACACTTCCGTTCGACGGCAGTTACTCAAAACTTCAAAGGGCTGGTGGCGATCGAGAAAAGCTCGACCTTGGACTCGTAGCCGTCAACTTTAACATTCCCAAGTTGTTTGACTACAGCTGCCCCAACTCCACGCAAACGCTAAGCGACGTTGCAAAGGCATGCGTTGTAGTCATTCAGACCATTTCTGAGGCTGCAAGATTTCAAAATATTGAGAACGCAGTTCAAAACAACATGCCACCAACTCGAGAAATTCTATCTTTGGAGAATAATTGGGGTGCTCTCTCGAAACAAGTTCAGCTGGCAGACCCTAATACTGGACGGTTTAAAGCTACCGTTCAGCTTATTGGCGAGACAGGTAATCCAGTTAATGTAATGGATGTCAATTCACCGTATGTAAAAGGCAACCTCCAGTTGCTGCTGAACCAACAGAATGTTCAACCTATGCCTACACTGTAA

Protein sequence

MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLRATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVFTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATVQLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPMPTL

Homology

[Close]

BLAST of Moc07g03960 vs. NCBI nr
Match: XP_022158194.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 557.0 bits (1434), Expect = 9.3e-155
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60
           MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL
Sbjct: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60

Query: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120
           RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV
Sbjct: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120

Query: 121 FTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAK 180
           FTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAK
Sbjct: 121 FTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAK 180

Query: 181 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 240
           ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV
Sbjct: 181 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 240

Query: 241 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPMPTL 280
           QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPMPTL
Sbjct: 241 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPMPTL 279

[Close]

BLAST of Moc07g03960 vs. NCBI nr
Match: XP_022156706.1 (ribosome-inactivating protein bryodin I-like, partial [Momordica charantia])

HSP 1 Score: 348.2 bits (892), Expect = 6.6e-92
Identity = 178/275 (64.73%), Postives = 211/275 (76.73%), Query Frame = 0


Query: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61
           KL+VF LLI+ IF A+P+VEGDVSF L   TK SYGAFL  FR D+P+E+K  + I LLR
Sbjct: 3   KLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISLLR 62

Query: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121
           ATV GA RYTRIHL+DR    ITLAIDVTNVY+LGFY+P+C++SHFFND  +I AK +VF
Sbjct: 63  ATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSFVF 122

Query: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKA 181
           TNSK Q TLPFDGSY +L   G +R  L LGL ++N  IP L  Y+CPN+    +DVAKA
Sbjct: 123 TNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKA 182

Query: 182 CVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATVQ 241
           CVV IQT+SEAARFQ I++AV NN+ P  EILSLENNWG LSKQVQLA+ N GRFK+ V 
Sbjct: 183 CVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNVT 242

Query: 242 LIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPM 277
           L   TG  V V +VNSPYV+GN++LLLNQQNVQ M
Sbjct: 243 LQDPTGATVIVSNVNSPYVRGNIRLLLNQQNVQAM 277

[Close]

BLAST of Moc07g03960 vs. NCBI nr
Match: XP_022158190.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 326.2 bits (835), Expect = 2.7e-85
Identity = 173/273 (63.37%), Postives = 207/273 (75.82%), Query Frame = 0


Query: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61
           KL+VFCLLI+A+F AAP+VEGDVSF L GVTK SY  FL  +R D+P + +TVF I LL 
Sbjct: 3   KLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIILLP 62

Query: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121
             VTG++RYTRIHL++  + TI LAIDVTNVYVLGFYT +C++SHFFNDS A +AKK+VF
Sbjct: 63  VNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFVF 122

Query: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNF-NIPKLFDYSCPNSTQTLSDVAK 181
           T+SK Q TL FDGSY +L  AGG R+ L LGLV +N+  IP L+ Y+CP  +   SDVAK
Sbjct: 123 TDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 182

Query: 182 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 241
           A VVVIQTISEAARFQ I+  V+ N  PT E+LSLENNW  LSK V+ ADP TG FK   
Sbjct: 183 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 242

Query: 242 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 274
            L   TG PV V +VNSPYVKGN+QLLLN+QN+
Sbjct: 243 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNI 273

[Close]

BLAST of Moc07g03960 vs. NCBI nr
Match: XP_022158438.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 231.1 bits (588), Expect = 1.2e-56
Identity = 129/271 (47.60%), Postives = 173/271 (63.84%), Query Frame = 0


Query: 2   KLIVFCLLIIAIFHAAP--SVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPL 61
           KL VFCLL +AIF AAP  +V+G    T+      SY + L  FR  +  ++K V  IPL
Sbjct: 3   KLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALR-DNKKVCNIPL 62

Query: 62  LRATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYT--PKCDVSHFFNDSAAIQAK 121
           LRA V   NR+  I ++   + TI LAID+ NVYVLG+YT  P C   H+F+D  A++A 
Sbjct: 63  LRANVIDKNRFIEISIAS-TNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVRAT 122

Query: 122 KYVFTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSD 181
           +YVF N   Q +LPFDG Y   ++ G +R  LDLGL ++N  +P L++Y C  +    +D
Sbjct: 123 QYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQTD 182

Query: 182 VAKACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFK 241
           VAKA VV +Q +SEAARF+ I+ AV NN  P  E+LSL+N+W  LS+ VQ AD   GRFK
Sbjct: 183 VAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGRFK 242

Query: 242 ATVQLIGETGNPVNVMDVNSPYVKGNLQLLL 269
             V L  E G+PV+V +++S YV GNL+LLL
Sbjct: 243 DKVTLNDEKGHPVDVWNISSKYVSGNLKLLL 271

[Close]

BLAST of Moc07g03960 vs. NCBI nr
Match: P33185.3 (RecName: Full=Ribosome-inactivating protein bryodin I; AltName: Full=BD1; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica] >AAL34334.1 ribosome-inactivating protein [Bryonia dioica])

HSP 1 Score: 223.0 bits (567), Expect = 3.2e-54
Identity = 122/278 (43.88%), Postives = 182/278 (65.47%), Query Frame = 0


Query: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60
           +KL+V  LLI+ IF  +P+VEGDVSF L G T  SYG F+K  R  +P E K V+ IPLL
Sbjct: 2   IKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERK-VYNIPLL 61

Query: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120
           R++++G+ RYT +HL++  D TI++A+DVTNVY++G+     DVS+FFN+++A +A K+V
Sbjct: 62  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAG--DVSYFFNEASATEAAKFV 121

Query: 121 FTNSKGQITLPFDGSYSKLQRAGGD-REKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVA 180
           F ++K ++TLP+ G+Y +LQ A G  RE + LGL A++  I  L+ Y       T S  A
Sbjct: 122 FKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYY-------TASSAA 181

Query: 181 KACVVVIQTISEAARF----QNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGR 240
            A +V+IQ+ +E+AR+    Q I   V     P+   +SLENNW ALSKQ+Q+A  N G+
Sbjct: 182 SALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQ 241

Query: 241 FKATVQLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 274
           F++ V LI      V++ + ++  V  N+ LLLN+ N+
Sbjct: 242 FESPVVLIDGNNQRVSITNASARVVTSNIALLLNRNNI 269

[Close]

BLAST of Moc07g03960 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 223.0 bits (567), Expect = 4.2e-57
Identity = 122/278 (43.88%), Postives = 182/278 (65.47%), Query Frame = 0


Query: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60
           +KL+V  LLI+ IF  +P+VEGDVSF L G T  SYG F+K  R  +P E K V+ IPLL
Sbjct: 2   IKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERK-VYNIPLL 61

Query: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120
           R++++G+ RYT +HL++  D TI++A+DVTNVY++G+     DVS+FFN+++A +A K+V
Sbjct: 62  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAG--DVSYFFNEASATEAAKFV 121

Query: 121 FTNSKGQITLPFDGSYSKLQRAGGD-REKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVA 180
           F ++K ++TLP+ G+Y +LQ A G  RE + LGL A++  I  L+ Y       T S  A
Sbjct: 122 FKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYY-------TASSAA 181

Query: 181 KACVVVIQTISEAARF----QNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGR 240
            A +V+IQ+ +E+AR+    Q I   V     P+   +SLENNW ALSKQ+Q+A  N G+
Sbjct: 182 SALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQ 241

Query: 241 FKATVQLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 274
           F++ V LI      V++ + ++  V  N+ LLLN+ N+
Sbjct: 242 FESPVVLIDGNNQRVSITNASARVVTSNIALLLNRNNI 269

[Close]

BLAST of Moc07g03960 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 1.6e-56
Identity = 124/278 (44.60%), Postives = 178/278 (64.03%), Query Frame = 0


Query: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60
           ++ +VF LLI+ +F  AP+VEGDVSF L G T  SYG F+   R  +P E K ++ IPLL
Sbjct: 2   IRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERK-LYDIPLL 61

Query: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120
           R+T+ G+ RY  IHL++  D TI++AIDVTNVYV+G+     D S+FFN+++A +A KYV
Sbjct: 62  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAG--DTSYFFNEASATEAAKYV 121

Query: 121 FTNSKGQITLPFDGSYSKLQRAGGD-REKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVA 180
           F ++K ++TLP+ G+Y +LQ A G  RE + LGL A++  I  LF Y+        +  A
Sbjct: 122 FKDAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNA-------NSAA 181

Query: 181 KACVVVIQTISEAARF----QNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGR 240
            A +V+IQ+ SEAAR+    Q I   V     P+  I+SLEN+W ALSKQ+Q+A  N G+
Sbjct: 182 SALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQ 241

Query: 241 FKATVQLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 274
           F+  V LI      V + +V++  V  N+ LLLN+ N+
Sbjct: 242 FETPVVLINAQNQRVTITNVDAGVVTSNIALLLNRNNM 269

[Close]

BLAST of Moc07g03960 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 1.5e-54
Identity = 120/281 (42.70%), Postives = 177/281 (62.99%), Query Frame = 0


Query: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60
           ++ +V  LLI+ +F   P+VEGDVSF L G T  SYG F+   R  +P E K ++ IPLL
Sbjct: 2   IRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERK-LYDIPLL 61

Query: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120
           R+++ G+ RY  IHL++  D TI++AIDVTNVY++G+     D S+FFN+++A +A KYV
Sbjct: 62  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAG--DTSYFFNEASATEAAKYV 121

Query: 121 FTNSKGQITLPFDGSYSKLQRAGGD-REKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVA 180
           F ++  ++TLP+ G+Y +LQ A G  RE + LGL A++  I  LF Y+        +  A
Sbjct: 122 FKDAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNA-------NSAA 181

Query: 181 KACVVVIQTISEAARF----QNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGR 240
            A +V+IQ+ SEAAR+    Q I   V     P+  I+SLEN+W ALSKQ+Q+A  N G+
Sbjct: 182 SALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQ 241

Query: 241 FKATVQLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPM 277
           F++ V LI      V + +V++  V  N+ LLLN+ N+  M
Sbjct: 242 FESPVVLINAQNQRVTITNVDAGVVTSNIALLLNRNNMAAM 272

[Close]

BLAST of Moc07g03960 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 206.1 bits (523), Expect = 5.3e-52
Identity = 119/274 (43.43%), Postives = 167/274 (60.95%), Query Frame = 0


Query: 5   VFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLRATV 64
           V   LI+AIF     V+GDVSF L G   +SYG F+K  R  +P   K V+ IPLL  +V
Sbjct: 6   VLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREK-VYNIPLLLPSV 65

Query: 65  TGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVFTNS 124
           +GA RY  +HL + D  TIT+A+DVTNVY++G+       S+FFN+ AA  A +YVF ++
Sbjct: 66  SGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLAD--TTSYFFNEPAAELASQYVFRDA 125

Query: 125 KGQITLPFDGSYSKLQ-RAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKACV 184
           + +ITLP+ G+Y +LQ  AG  REK+ +GL A++  I  L  Y         +  A A +
Sbjct: 126 RRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDS-------TAAAGALL 185

Query: 185 VVIQTISEAARFQNIENAVQ----NNMPPTREILSLENNWGALSKQVQLADPNTGRFKAT 244
           V+IQT +EAARF+ IE  +Q     +  P+   +SLEN+W  LSKQ+QLA  N G F+  
Sbjct: 186 VLIQTTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTP 245

Query: 245 VQLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 274
           + L+   GN V + +V S  V  N+QLLLN +N+
Sbjct: 246 IVLVDNKGNRVQITNVTSKVVTSNIQLLLNTRNI 269

[Close]

BLAST of Moc07g03960 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.5e-48
Identity = 120/274 (43.80%), Postives = 165/274 (60.22%), Query Frame = 0


Query: 6   FCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLRATVT 65
           F +LI+AIF AA +VE DV F+L G +  SY  F+   R  +P  + TV+ I LL ++ +
Sbjct: 4   FTVLILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALP-SNGTVYNITLLLSSAS 63

Query: 66  GANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVFTNSK 125
           GA+RYT + LS+ D   IT+A+DVTNVY++G+       S+FFN+S A  A +YVF  S 
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVN--STSYFFNESDAKLASQYVFKGST 123

Query: 126 GQITLPFDGSYSKLQRAGGD-REKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKACVV 185
             +TLP+ G+Y KLQ A G  REK+ LG  A++  I  LF Y         +  A A +V
Sbjct: 124 -IVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDS-------TAAAAAFLV 183

Query: 186 VIQTISEAARFQNIE----NAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 245
           +IQT +EA+RF+ IE      +  N  P+   +SLEN W ALSKQ+QLA  N G FK  V
Sbjct: 184 IIQTTAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPV 243

Query: 246 QLIGETGNPVNVMDVNSPYVKGNLQLLLN-QQNV 274
            +  + G  V + +V S  V  N+QLLLN +QNV
Sbjct: 244 VITDDKGQRVEITNVTSKVVTKNIQLLLNYKQNV 266

[Close]

BLAST of Moc07g03960 vs. ExPASy TrEMBL
Match: A0A6J1DYP1 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.5e-155
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60
           MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL
Sbjct: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60

Query: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120
           RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV
Sbjct: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120

Query: 121 FTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAK 180
           FTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAK
Sbjct: 121 FTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAK 180

Query: 181 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 240
           ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV
Sbjct: 181 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 240

Query: 241 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPMPTL 280
           QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPMPTL
Sbjct: 241 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPMPTL 279

[Close]

BLAST of Moc07g03960 vs. ExPASy TrEMBL
Match: A0A6J1DVR9 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 3.2e-92
Identity = 178/275 (64.73%), Postives = 211/275 (76.73%), Query Frame = 0


Query: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61
           KL+VF LLI+ IF A+P+VEGDVSF L   TK SYGAFL  FR D+P+E+K  + I LLR
Sbjct: 3   KLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISLLR 62

Query: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121
           ATV GA RYTRIHL+DR    ITLAIDVTNVY+LGFY+P+C++SHFFND  +I AK +VF
Sbjct: 63  ATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSFVF 122

Query: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKA 181
           TNSK Q TLPFDGSY +L   G +R  L LGL ++N  IP L  Y+CPN+    +DVAKA
Sbjct: 123 TNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKA 182

Query: 182 CVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATVQ 241
           CVV IQT+SEAARFQ I++AV NN+ P  EILSLENNWG LSKQVQLA+ N GRFK+ V 
Sbjct: 183 CVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNVT 242

Query: 242 LIGETGNPVNVMDVNSPYVKGNLQLLLNQQNVQPM 277
           L   TG  V V +VNSPYV+GN++LLLNQQNVQ M
Sbjct: 243 LQDPTGATVIVSNVNSPYVRGNIRLLLNQQNVQAM 277

[Close]

BLAST of Moc07g03960 vs. ExPASy TrEMBL
Match: A0A6J1DV53 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.3e-85
Identity = 173/273 (63.37%), Postives = 207/273 (75.82%), Query Frame = 0


Query: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61
           KL+VFCLLI+A+F AAP+VEGDVSF L GVTK SY  FL  +R D+P + +TVF I LL 
Sbjct: 3   KLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIILLP 62

Query: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121
             VTG++RYTRIHL++  + TI LAIDVTNVYVLGFYT +C++SHFFNDS A +AKK+VF
Sbjct: 63  VNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFVF 122

Query: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNF-NIPKLFDYSCPNSTQTLSDVAK 181
           T+SK Q TL FDGSY +L  AGG R+ L LGLV +N+  IP L+ Y+CP  +   SDVAK
Sbjct: 123 TDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 182

Query: 182 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 241
           A VVVIQTISEAARFQ I+  V+ N  PT E+LSLENNW  LSK V+ ADP TG FK   
Sbjct: 183 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 242

Query: 242 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 274
            L   TG PV V +VNSPYVKGN+QLLLN+QN+
Sbjct: 243 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNI 273

[Close]

BLAST of Moc07g03960 vs. ExPASy TrEMBL
Match: A0A6J1DW33 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 5.7e-57
Identity = 129/271 (47.60%), Postives = 173/271 (63.84%), Query Frame = 0


Query: 2   KLIVFCLLIIAIFHAAP--SVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPL 61
           KL VFCLL +AIF AAP  +V+G    T+      SY + L  FR  +  ++K V  IPL
Sbjct: 3   KLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALR-DNKKVCNIPL 62

Query: 62  LRATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYT--PKCDVSHFFNDSAAIQAK 121
           LRA V   NR+  I ++   + TI LAID+ NVYVLG+YT  P C   H+F+D  A++A 
Sbjct: 63  LRANVIDKNRFIEISIAS-TNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVRAT 122

Query: 122 KYVFTNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSD 181
           +YVF N   Q +LPFDG Y   ++ G +R  LDLGL ++N  +P L++Y C  +    +D
Sbjct: 123 QYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQTD 182

Query: 182 VAKACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFK 241
           VAKA VV +Q +SEAARF+ I+ AV NN  P  E+LSL+N+W  LS+ VQ AD   GRFK
Sbjct: 183 VAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGRFK 242

Query: 242 ATVQLIGETGNPVNVMDVNSPYVKGNLQLLL 269
             V L  E G+PV+V +++S YV GNL+LLL
Sbjct: 243 DKVTLNDEKGHPVDVWNISSKYVSGNLKLLL 271

[Close]

BLAST of Moc07g03960 vs. ExPASy TrEMBL
Match: Q53X19 (rRNA N-glycosidase OS=Bryonia dioica OX=3652 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.5e-54
Identity = 122/278 (43.88%), Postives = 182/278 (65.47%), Query Frame = 0


Query: 1   MKLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLL 60
           +KL+V  LLI+ IF  +P+VEGDVSF L G T  SYG F+K  R  +P E K V+ IPLL
Sbjct: 2   IKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERK-VYNIPLL 61

Query: 61  RATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYV 120
           R++++G+ RYT +HL++  D TI++A+DVTNVY++G+     DVS+FFN+++A +A K+V
Sbjct: 62  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAG--DVSYFFNEASATEAAKFV 121

Query: 121 FTNSKGQITLPFDGSYSKLQRAGGD-REKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVA 180
           F ++K ++TLP+ G+Y +LQ A G  RE + LGL A++  I  L+ Y       T S  A
Sbjct: 122 FKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYY-------TASSAA 181

Query: 181 KACVVVIQTISEAARF----QNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGR 240
            A +V+IQ+ +E+AR+    Q I   V     P+   +SLENNW ALSKQ+Q+A  N G+
Sbjct: 182 SALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQ 241

Query: 241 FKATVQLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 274
           F++ V LI      V++ + ++  V  N+ LLLN+ N+
Sbjct: 242 FESPVVLIDGNNQRVSITNASARVVTSNIALLLNRNNI 269

The following BLAST results are available for this feature:

BLAST of Moc07g03960 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158194.1	9.3e-155	100.00	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022156706.1	6.6e-92	64.73	ribosome-inactivating protein bryodin I-like, partial [Momordica charantia]	[more]
XP_022158190.1	2.7e-85	63.37	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022158438.1	1.2e-56	47.60	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]
P33185.3	3.2e-54	43.88	RecName: Full=Ribosome-inactivating protein bryodin I; AltName: Full=BD1; AltNam...	[more]

BLAST of Moc07g03960 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33185	4.2e-57	43.88	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P24478	1.6e-56	44.60	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	1.5e-54	42.70	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P16094	5.3e-52	43.43	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
Q00465	3.5e-48	43.80	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of Moc07g03960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYP1	4.5e-155	100.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1	[more]
A0A6J1DVR9	3.2e-92	64.73	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1	[more]
A0A6J1DV53	1.3e-85	63.37	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1	[more]
A0A6J1DW33	5.7e-57	47.60	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1	[more]
Q53X19	1.5e-54	43.88	rRNA N-glycosidase OS=Bryonia dioica OX=3652 PE=2 SV=1	[more]

BLAST of Moc07g03960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 182..202 score: 52.01 coord: 82..97 score: 55.76 coord: 146..160 score: 35.79 coord: 34..47 score: 33.27 coord: 210..227 score: 46.2
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 195..274 e-value: 1.8E-21 score: 77.8
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 32..227 e-value: 3.6E-32 score: 111.6
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 24..272
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 22..194 e-value: 2.8E-39 score: 136.7
None	No IPR available	PIRSR	PIRSR001924-1	PIRSR001924-1	coord: 1..223 e-value: 6.2E-8 score: 30.1
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 24..272
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 186..202
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..270

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g03960.1	Moc07g03960.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
cellular_component	GO:0016020	membrane
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity