Moc07g03940 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc07g03940
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	rRNA N-glycosidase
Location	chr7: 3445045 .. 3445896 (+)
RNA-Seq Expression	Moc07g03940
Synteny	Moc07g03940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCAAATTGTTAGTGTTTTGTTTGCTAATTCTCGCGGTCTTCCTTGCAGCTCCTACGGTCGAGGGCGATGTTAGCTTCAATTTGTCCGGTGTTACCAAAACATCCTATGCAACTTTCCTCAATGCTTATAGAGCCGATCTTCCATACAAAGAGACAGTGTTCGATATAATTCTACTGCCTGTCAATGTTACAGGTTCAAGTCGCTACACACGAATCCATCTTACAAATTCATACAATCAAACCATCCAGCTTGCTATAGATGTAACGAACGTTTATGTTCTGGGATTTTACACCACAGAGTGTAACTTATCCCACTTTTTCAACGACTCTGTTGCCACTGAAGCTAAGAAATTTGTATTCACCGATTCCAAGCAGCAAACACTTTCATTCGATGGCAGTTACGGACAACTTGCAGGAGCTGGAGGAAGACGACAAAGTCTTAAACTTGGACTCGTAGACCTCAACTATGTCTACATTCCAACGTTGTACGGCTACAACTGCCCCGGCTACAGTTACAAGCCGAGCGATGTTGCGAAGGCAATGGTCGTCGTAATTCAGACTATTTCCGAGGCTGCAAGATTTCAAAAGATTCAGAAAGATGTTAAATACAACAGTATTCCAACTGCAGAAGTTCTGTCATTGGAAAATAATTGGAGCAAACTCTCCAAACTAGTTAAGCAAGCAGACCCGACAACTGGAAACTTTAAGGTTAACTTTACACTTGAAAACGCGACAGGTACTCCAGTTGTTGTATCCAACGTTAATTCACCATATGTAAAAGGTAACATCCAGCTGCTGCTGAACAAACAGAATATTTCAGCTACGTCTGAAAATTACGCTACAATCTAA

mRNA sequence

ATGACCAAATTGTTAGTGTTTTGTTTGCTAATTCTCGCGGTCTTCCTTGCAGCTCCTACGGTCGAGGGCGATGTTAGCTTCAATTTGTCCGGTGTTACCAAAACATCCTATGCAACTTTCCTCAATGCTTATAGAGCCGATCTTCCATACAAAGAGACAGTGTTCGATATAATTCTACTGCCTGTCAATGTTACAGGTTCAAGTCGCTACACACGAATCCATCTTACAAATTCATACAATCAAACCATCCAGCTTGCTATAGATGTAACGAACGTTTATGTTCTGGGATTTTACACCACAGAGTGTAACTTATCCCACTTTTTCAACGACTCTGTTGCCACTGAAGCTAAGAAATTTGTATTCACCGATTCCAAGCAGCAAACACTTTCATTCGATGGCAGTTACGGACAACTTGCAGGAGCTGGAGGAAGACGACAAAGTCTTAAACTTGGACTCGTAGACCTCAACTATGTCTACATTCCAACGTTGTACGGCTACAACTGCCCCGGCTACAGTTACAAGCCGAGCGATGTTGCGAAGGCAATGGTCGTCGTAATTCAGACTATTTCCGAGGCTGCAAGATTTCAAAAGATTCAGAAAGATGTTAAATACAACAGTATTCCAACTGCAGAAGTTCTGTCATTGGAAAATAATTGGAGCAAACTCTCCAAACTAGTTAAGCAAGCAGACCCGACAACTGGAAACTTTAAGGTTAACTTTACACTTGAAAACGCGACAGGTACTCCAGTTGTTGTATCCAACGTTAATTCACCATATGTAAAAGGTAACATCCAGCTGCTGCTGAACAAACAGAATATTTCAGCTACGTCTGAAAATTACGCTACAATCTAA

Coding sequence (CDS)

ATGACCAAATTGTTAGTGTTTTGTTTGCTAATTCTCGCGGTCTTCCTTGCAGCTCCTACGGTCGAGGGCGATGTTAGCTTCAATTTGTCCGGTGTTACCAAAACATCCTATGCAACTTTCCTCAATGCTTATAGAGCCGATCTTCCATACAAAGAGACAGTGTTCGATATAATTCTACTGCCTGTCAATGTTACAGGTTCAAGTCGCTACACACGAATCCATCTTACAAATTCATACAATCAAACCATCCAGCTTGCTATAGATGTAACGAACGTTTATGTTCTGGGATTTTACACCACAGAGTGTAACTTATCCCACTTTTTCAACGACTCTGTTGCCACTGAAGCTAAGAAATTTGTATTCACCGATTCCAAGCAGCAAACACTTTCATTCGATGGCAGTTACGGACAACTTGCAGGAGCTGGAGGAAGACGACAAAGTCTTAAACTTGGACTCGTAGACCTCAACTATGTCTACATTCCAACGTTGTACGGCTACAACTGCCCCGGCTACAGTTACAAGCCGAGCGATGTTGCGAAGGCAATGGTCGTCGTAATTCAGACTATTTCCGAGGCTGCAAGATTTCAAAAGATTCAGAAAGATGTTAAATACAACAGTATTCCAACTGCAGAAGTTCTGTCATTGGAAAATAATTGGAGCAAACTCTCCAAACTAGTTAAGCAAGCAGACCCGACAACTGGAAACTTTAAGGTTAACTTTACACTTGAAAACGCGACAGGTACTCCAGTTGTTGTATCCAACGTTAATTCACCATATGTAAAAGGTAACATCCAGCTGCTGCTGAACAAACAGAATATTTCAGCTACGTCTGAAAATTACGCTACAATCTAA

Protein sequence

MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILLPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFVFTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAKAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSENYATI

Homology

[Close]

BLAST of Moc07g03940 vs. NCBI nr
Match: XP_022158190.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 552.4 bits (1422), Expect = 2.3e-153
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL
Sbjct: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
           PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV
Sbjct: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120

Query: 121 FTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 180
           FTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK
Sbjct: 121 FTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 180

Query: 181 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 240
           AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF
Sbjct: 181 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 240

Query: 241 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSENYATI 284
           TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSENYATI
Sbjct: 241 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSENYATI 283

[Close]

BLAST of Moc07g03940 vs. NCBI nr
Match: XP_022156706.1 (ribosome-inactivating protein bryodin I-like, partial [Momordica charantia])

HSP 1 Score: 346.3 bits (887), Expect = 2.5e-91
Identity = 187/287 (65.16%), Postives = 222/287 (77.35%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIIL 60
           M KLLVF LLIL +FLA+PTVEGDVSFNLS  TKTSY  FLNA+RADLP + +  ++I L
Sbjct: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60

Query: 61  LPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKF 120
           L   V G++RYTRIHLT+   + I LAIDVTNVY+LGFY+ +CN+SHFFND V+  AK F
Sbjct: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120

Query: 121 VFTDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           VFT+SK+Q TL FDGSY QLAG G  R SL LGL  LN  +IPTL GYNCP  +Y+P+DV
Sbjct: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNN-FIPTLAGYNCPNTNYQPADV 180

Query: 181 AKAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKV 240
           AKA VV IQT+SEAARFQKIQ  V  N +P AE+LSLENNW KLSK V+ A+   G FK 
Sbjct: 181 AKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKS 240

Query: 241 NFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATS--ENYATI 284
           N TL++ TG  V+VSNVNSPYV+GNI+LLLN+QN+ A S  ENYAT+
Sbjct: 241 NVTLQDPTGATVIVSNVNSPYVRGNIRLLLNQQNVQAMSEHENYATM 286

[Close]

BLAST of Moc07g03940 vs. NCBI nr
Match: XP_022158194.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 319.3 bits (817), Expect = 3.3e-83
Identity = 173/273 (63.37%), Postives = 207/273 (75.82%), Query Frame = 0


Query: 3   KLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIILLP 62
           KL+VFCLLI+A+F AAP+VEGDVSF L GVTK SY  FL  +R D+P + +TVF I LL 
Sbjct: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61

Query: 63  VNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFVF 122
             VTG++RYTRIHL++  + TI LAIDVTNVYVLGFYT +C++SHFFNDS A +AKK+VF
Sbjct: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121

Query: 123 TDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 182
           T+SK Q TL FDGSY +L  AGG R+ L LGLV +N+  IP L+ Y+CP  +   SDVAK
Sbjct: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNF-NIPKLFDYSCPNSTQTLSDVAK 181

Query: 183 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 242
           A VVVIQTISEAARFQ I+  V+ N  PT E+LSLENNW  LSK V+ ADP TG FK   
Sbjct: 182 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 241

Query: 243 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNI 274
            L   TG PV V +VNSPYVKGN+QLLLN+QN+
Sbjct: 242 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 273

[Close]

BLAST of Moc07g03940 vs. NCBI nr
Match: XP_022158438.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 217.2 bits (552), Expect = 1.8e-52
Identity = 137/298 (45.97%), Postives = 179/298 (60.07%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAP--TVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDII 60
           M KL VFCLL LA+FLAAP  TV+G     +S     SY + LN +R  L   + V +I 
Sbjct: 1   MRKLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALRDNKKVCNIP 60

Query: 61  LLPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYT--TECNLSHFFNDSVATEA 120
           LL  NV   +R+  I +  S N TI LAID+ NVYVLG+YT    C+  H+F+D VA  A
Sbjct: 61  LLRANVIDKNRFIEISIA-STNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVRA 120

Query: 121 KKFVFTD-SKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKP 180
            ++VF + ++Q++L FDG Y      G  R+SL LGL  LN++ +P LY Y C G  Y  
Sbjct: 121 TQYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHL-LPALYEYGCTGTKYIQ 180

Query: 181 SDVAKAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGN 240
           +DVAKA+VV +Q +SEAARF+ IQ  V  N  P AEVLSL+N+WS LS+ V++AD   G 
Sbjct: 181 TDVAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGR 240

Query: 241 FKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISAT----------SENYATI 284
           FK   TL +  G PV V N++S YV GN++LLL      A            +NYATI
Sbjct: 241 FKDKVTLNDEKGHPVDVWNISSKYVSGNLKLLLFVNTTFAALPSVVDNNGGHQNYATI 296

[Close]

BLAST of Moc07g03940 vs. NCBI nr
Match: P33185.3 (RecName: Full=Ribosome-inactivating protein bryodin I; AltName: Full=BD1; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica] >AAL34334.1 ribosome-inactivating protein [Bryonia dioica])

HSP 1 Score: 210.3 bits (534), Expect = 2.2e-50
Identity = 127/285 (44.56%), Postives = 182/285 (63.86%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           M KLLV  LLIL +FL +PTVEGDVSF LSG T TSY  F+   R  LPY+  V++I LL
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
             +++GS RYT +HLTN  ++TI +A+DVTNVY++G+   +  +S+FFN++ ATEA KFV
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGD--VSYFFNEASATEAAKFV 120

Query: 121 FTDSKQQ-TLSFDGSYGQLAGAGGR-RQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           F D+K++ TL + G+Y +L  A G+ R+++ LGL  L+   I TLY Y         S  
Sbjct: 121 FKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSA-ITTLYYYTA-------SSA 180

Query: 181 AKAMVVVIQTISEAARF----QKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTG 240
           A A++V+IQ+ +E+AR+    Q+I K V    +P+   +SLENNWS LSK ++ A    G
Sbjct: 181 ASALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNG 240

Query: 241 NFKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSEN 280
            F+    L +     V ++N ++  V  NI LLLN+ NI+A  E+
Sbjct: 241 QFESPVVLIDGNNQRVSITNASARVVTSNIALLLNRNNIAAIGED 275

[Close]

BLAST of Moc07g03940 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 210.3 bits (534), Expect = 2.8e-53
Identity = 127/285 (44.56%), Postives = 182/285 (63.86%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           M KLLV  LLIL +FL +PTVEGDVSF LSG T TSY  F+   R  LPY+  V++I LL
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
             +++GS RYT +HLTN  ++TI +A+DVTNVY++G+   +  +S+FFN++ ATEA KFV
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGD--VSYFFNEASATEAAKFV 120

Query: 121 FTDSKQQ-TLSFDGSYGQLAGAGGR-RQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           F D+K++ TL + G+Y +L  A G+ R+++ LGL  L+   I TLY Y         S  
Sbjct: 121 FKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSA-ITTLYYYTA-------SSA 180

Query: 181 AKAMVVVIQTISEAARF----QKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTG 240
           A A++V+IQ+ +E+AR+    Q+I K V    +P+   +SLENNWS LSK ++ A    G
Sbjct: 181 ASALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNG 240

Query: 241 NFKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSEN 280
            F+    L +     V ++N ++  V  NI LLLN+ NI+A  E+
Sbjct: 241 QFESPVVLIDGNNQRVSITNASARVVTSNIALLLNRNNIAAIGED 275

[Close]

BLAST of Moc07g03940 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 8.3e-53
Identity = 127/285 (44.56%), Postives = 182/285 (63.86%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           M + LVF LLIL +FL AP VEGDVSF LSG T +SY  F++  R  LPY+  ++DI LL
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
              + GS RY  IHLTN  ++TI +AIDVTNVYV+G+   +   S+FFN++ ATEA K+V
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGD--TSYFFNEASATEAAKYV 120

Query: 121 FTDSKQQ-TLSFDGSYGQLAGAGGR-RQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           F D+K++ TL + G+Y +L  A G+ R+++ LGL  L+   I TL+ YN        +  
Sbjct: 121 FKDAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSA-ITTLFYYNA-------NSA 180

Query: 181 AKAMVVVIQTISEAARF----QKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTG 240
           A A++V+IQ+ SEAAR+    Q+I K V    +P+  ++SLEN+WS LSK ++ A    G
Sbjct: 181 ASALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNG 240

Query: 241 NFKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSEN 280
            F+    L NA    V ++NV++  V  NI LLLN+ N++A  ++
Sbjct: 241 QFETPVVLINAQNQRVTITNVDAGVVTSNIALLLNRNNMAAIDDD 275

[Close]

BLAST of Moc07g03940 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 6.5e-50
Identity = 122/285 (42.81%), Postives = 179/285 (62.81%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           M + LV  LLIL +FL  P VEGDVSF LSG T +SY  F++  R  LP +  ++DI LL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
             ++ GS RY  IHLTN  ++TI +AIDVTNVY++G+   +   S+FFN++ ATEA K+V
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGD--TSYFFNEASATEAAKYV 120

Query: 121 FTDS-KQQTLSFDGSYGQLAGAGGR-RQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           F D+ ++ TL + G+Y +L  A G+ R+++ LGL  L+   I TL+ YN        +  
Sbjct: 121 FKDAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSA-ITTLFYYNA-------NSA 180

Query: 181 AKAMVVVIQTISEAARF----QKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTG 240
           A A++V+IQ+ SEAAR+    Q+I K V    +P+  ++SLEN+WS LSK ++ A    G
Sbjct: 181 ASALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNG 240

Query: 241 NFKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSEN 280
            F+    L NA    V ++NV++  V  NI LLLN+ N++A  ++
Sbjct: 241 QFESPVVLINAQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDD 275

[Close]

BLAST of Moc07g03940 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.1e-44
Identity = 114/279 (40.86%), Postives = 169/279 (60.57%), Query Frame = 0


Query: 7   FCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILLPVNVTG 66
           F +LILA+F+AA TVE DV F+LSG + TSY+ F+   R  LP   TV++I LL  + +G
Sbjct: 4   FTVLILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSASG 63

Query: 67  SSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFVFTDSKQ 126
           +SRYT + L+N   + I +A+DVTNVY++G+     + S+FFN+S A  A ++VF  S  
Sbjct: 64  ASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVN--STSYFFNESDAKLASQYVFKGSTI 123

Query: 127 QTLSFDGSYGQLAGAGGR-RQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAKAMVVV 186
            TL + G+Y +L  A G+ R+ + LG   L+   I TL+ Y+        +  A A +V+
Sbjct: 124 VTLPYSGNYEKLQTAAGKIREKIPLGFPALDSA-ITTLFHYD-------STAAAAAFLVI 183

Query: 187 IQTISEAARFQKIQ----KDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNFT 246
           IQT +EA+RF+ I+    + +  N +P+   +SLEN WS LSK ++ A    G FK    
Sbjct: 184 IQTTAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVV 243

Query: 247 LENATGTPVVVSNVNSPYVKGNIQLLLN-KQNISATSEN 280
           + +  G  V ++NV S  V  NIQLLLN KQN++A  E+
Sbjct: 244 ITDDKGQRVEITNVTSKVVTKNIQLLLNYKQNVAAFDED 272

[Close]

BLAST of Moc07g03940 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 174.9 bits (442), Expect = 1.3e-42
Identity = 110/280 (39.29%), Postives = 166/280 (59.29%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           M++  V   LILA+FL    V+GDVSF LSG    SY  F+   R  LP++E V++I LL
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
             +V+G+ RY  +HL N   +TI +A+DVTNVY++G+       S+FFN+  A  A ++V
Sbjct: 61  LPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLAD--TTSYFFNEPAAELASQYV 120

Query: 121 FTDSKQQ-TLSFDGSYGQLAGAGGR-RQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           F D++++ TL + G+Y +L  A G+ R+ + +GL  L+      L+        Y  +  
Sbjct: 121 FRDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLH--------YDSTAA 180

Query: 181 AKAMVVVIQTISEAARF----QKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTG 240
           A A++V+IQT +EAARF    Q+IQ+    + +P+   +SLEN+WS LSK ++ A    G
Sbjct: 181 AGALLVLIQTTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNG 240

Query: 241 NFKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNIS 275
            F+    L +  G  V ++NV S  V  NIQLLLN +NI+
Sbjct: 241 IFRTPIVLVDNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

[Close]

BLAST of Moc07g03940 vs. ExPASy TrEMBL
Match: A0A6J1DV53 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.1e-153
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL
Sbjct: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
           PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV
Sbjct: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120

Query: 121 FTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 180
           FTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK
Sbjct: 121 FTDSKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 180

Query: 181 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 240
           AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF
Sbjct: 181 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 240

Query: 241 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSENYATI 284
           TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSENYATI
Sbjct: 241 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSENYATI 283

[Close]

BLAST of Moc07g03940 vs. ExPASy TrEMBL
Match: A0A6J1DVR9 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.2e-91
Identity = 187/287 (65.16%), Postives = 222/287 (77.35%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIIL 60
           M KLLVF LLIL +FLA+PTVEGDVSFNLS  TKTSY  FLNA+RADLP + +  ++I L
Sbjct: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60

Query: 61  LPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKF 120
           L   V G++RYTRIHLT+   + I LAIDVTNVY+LGFY+ +CN+SHFFND V+  AK F
Sbjct: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120

Query: 121 VFTDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           VFT+SK+Q TL FDGSY QLAG G  R SL LGL  LN  +IPTL GYNCP  +Y+P+DV
Sbjct: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNN-FIPTLAGYNCPNTNYQPADV 180

Query: 181 AKAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKV 240
           AKA VV IQT+SEAARFQKIQ  V  N +P AE+LSLENNW KLSK V+ A+   G FK 
Sbjct: 181 AKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKS 240

Query: 241 NFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATS--ENYATI 284
           N TL++ TG  V+VSNVNSPYV+GNI+LLLN+QN+ A S  ENYAT+
Sbjct: 241 NVTLQDPTGATVIVSNVNSPYVRGNIRLLLNQQNVQAMSEHENYATM 286

[Close]

BLAST of Moc07g03940 vs. ExPASy TrEMBL
Match: A0A6J1DYP1 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.6e-83
Identity = 173/273 (63.37%), Postives = 207/273 (75.82%), Query Frame = 0


Query: 3   KLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIILLP 62
           KL+VFCLLI+A+F AAP+VEGDVSF L GVTK SY  FL  +R D+P + +TVF I LL 
Sbjct: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61

Query: 63  VNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFVF 122
             VTG++RYTRIHL++  + TI LAIDVTNVYVLGFYT +C++SHFFNDS A +AKK+VF
Sbjct: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121

Query: 123 TDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDVAK 182
           T+SK Q TL FDGSY +L  AGG R+ L LGLV +N+  IP L+ Y+CP  +   SDVAK
Sbjct: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNF-NIPKLFDYSCPNSTQTLSDVAK 181

Query: 183 AMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKVNF 242
           A VVVIQTISEAARFQ I+  V+ N  PT E+LSLENNW  LSK V+ ADP TG FK   
Sbjct: 182 ACVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATV 241

Query: 243 TLENATGTPVVVSNVNSPYVKGNIQLLLNKQNI 274
            L   TG PV V +VNSPYVKGN+QLLLN+QN+
Sbjct: 242 QLIGETGNPVNVMDVNSPYVKGNLQLLLNQQNV 273

[Close]

BLAST of Moc07g03940 vs. ExPASy TrEMBL
Match: A0A6J1DW33 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 8.6e-53
Identity = 137/298 (45.97%), Postives = 179/298 (60.07%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAP--TVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDII 60
           M KL VFCLL LA+FLAAP  TV+G     +S     SY + LN +R  L   + V +I 
Sbjct: 1   MRKLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALRDNKKVCNIP 60

Query: 61  LLPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYT--TECNLSHFFNDSVATEA 120
           LL  NV   +R+  I +  S N TI LAID+ NVYVLG+YT    C+  H+F+D VA  A
Sbjct: 61  LLRANVIDKNRFIEISIA-STNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVRA 120

Query: 121 KKFVFTD-SKQQTLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKP 180
            ++VF + ++Q++L FDG Y      G  R+SL LGL  LN++ +P LY Y C G  Y  
Sbjct: 121 TQYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHL-LPALYEYGCTGTKYIQ 180

Query: 181 SDVAKAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGN 240
           +DVAKA+VV +Q +SEAARF+ IQ  V  N  P AEVLSL+N+WS LS+ V++AD   G 
Sbjct: 181 TDVAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGR 240

Query: 241 FKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISAT----------SENYATI 284
           FK   TL +  G PV V N++S YV GN++LLL      A            +NYATI
Sbjct: 241 FKDKVTLNDEKGHPVDVWNISSKYVSGNLKLLLFVNTTFAALPSVVDNNGGHQNYATI 296

[Close]

BLAST of Moc07g03940 vs. ExPASy TrEMBL
Match: Q53X19 (rRNA N-glycosidase OS=Bryonia dioica OX=3652 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.0e-50
Identity = 127/285 (44.56%), Postives = 182/285 (63.86%), Query Frame = 0


Query: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYKETVFDIILL 60
           M KLLV  LLIL +FL +PTVEGDVSF LSG T TSY  F+   R  LPY+  V++I LL
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKFV 120
             +++GS RYT +HLTN  ++TI +A+DVTNVY++G+   +  +S+FFN++ ATEA KFV
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGD--VSYFFNEASATEAAKFV 120

Query: 121 FTDSKQQ-TLSFDGSYGQLAGAGGR-RQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180
           F D+K++ TL + G+Y +L  A G+ R+++ LGL  L+   I TLY Y         S  
Sbjct: 121 FKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSA-ITTLYYYTA-------SSA 180

Query: 181 AKAMVVVIQTISEAARF----QKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTG 240
           A A++V+IQ+ +E+AR+    Q+I K V    +P+   +SLENNWS LSK ++ A    G
Sbjct: 181 ASALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNG 240

Query: 241 NFKVNFTLENATGTPVVVSNVNSPYVKGNIQLLLNKQNISATSEN 280
            F+    L +     V ++N ++  V  NI LLLN+ NI+A  E+
Sbjct: 241 QFESPVVLIDGNNQRVSITNASARVVTSNIALLLNRNNIAAIGED 275

The following BLAST results are available for this feature:

BLAST of Moc07g03940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158190.1	2.3e-153	100.00	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022156706.1	2.5e-91	65.16	ribosome-inactivating protein bryodin I-like, partial [Momordica charantia]	[more]
XP_022158194.1	3.3e-83	63.37	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022158438.1	1.8e-52	45.97	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]
P33185.3	2.2e-50	44.56	RecName: Full=Ribosome-inactivating protein bryodin I; AltName: Full=BD1; AltNam...	[more]

BLAST of Moc07g03940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33185	2.8e-53	44.56	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P24478	8.3e-53	44.56	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	6.5e-50	42.81	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
Q00465	4.1e-44	40.86	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P16094	1.3e-42	39.29	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]

BLAST of Moc07g03940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DV53	1.1e-153	100.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1	[more]
A0A6J1DVR9	1.2e-91	65.16	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1	[more]
A0A6J1DYP1	1.6e-83	63.37	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1	[more]
A0A6J1DW33	8.6e-53	45.97	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1	[more]
Q53X19	1.0e-50	44.56	rRNA N-glycosidase OS=Bryonia dioica OX=3652 PE=2 SV=1	[more]

BLAST of Moc07g03940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 35..48 score: 35.15 coord: 145..159 score: 30.0 coord: 210..227 score: 43.86 coord: 182..202 score: 47.62 coord: 82..97 score: 51.48
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..226 e-value: 1.4E-25 score: 90.1
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 25..273
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 195..274 e-value: 3.7E-19 score: 70.4
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 23..194 e-value: 1.4E-36 score: 127.9
None	No IPR available	PIRSR	PIRSR001924-1	PIRSR001924-1	coord: 9..226 e-value: 4.4E-8 score: 30.6
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 25..273
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 186..202
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..269

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc07g03940.1	Moc07g03940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
cellular_component	GO:0016020	membrane
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity